AT3G28480

prolyl 4-hydroxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009061
Physical Location & Seq
Reverse (-)
10675986 .. 10678388
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G28480.1

Sequence Viewer

Length: 951 bp
ATGGATTCTCGGATTTTTCTCGCATTTTCCCTCTGTTTTCTCTTCACTCTCCCTCTAATTTCATCTGCACCTAACCGCTTCCTCACTAGATCGAGTAACACTAGAGATGGGTCAGTGATAAAGATGAAAACAAGTGCTTCTTCTTTTGGGTTTGATCCAACTCGTGTCACTCAACTCTCTTGGACTCCTAGAGTTTTTTTGTATGAAGGGTTTCTTTCTGATGAGGAATGTGATCATTTTATCAAATTGGCGAAAGGGAAGCTTGAGAAGTCAATGGTGGCTGATAATGATTCTGGTGAGAGTGTTGAGAGTGAAGTACGCACTAGTTCTGGAATGTTTCTCTCCAAACGACAGGATGATATAGTAAGTAATGTGGAGGCAAAACTTGCTGCGTGGACCTTTCTTCCCGAAGAGAATGGTGAATCCATGCAAATATTGCATTATGAGAATGGTCAAAAGTATGAACCGCATTTTGACTACTTTCACGACCAGGCTAATCTAGAGCTCGGTGGTCATCGGATTGCCACAGTATTGATGTACTTGTCCAATGTTGAAAAGGGAGGAGAAACAGTGTTTCCCATGTGGAAGGGTAAAGCAACTCAGTTGAAAGACGATAGCTGGACCGAATGTGCAAAACAAGGCTATGCAGTGAAACCGAGGAAAGGGGATGCATTACTGTTCTTCAATCTTCACCCAAATGCAACCACAGATTCAAATAGCTTACATGGAAGCTGTCCAGTGGTTGAGGGAGAGAAATGGTCAGCAACCAGATGGATTCACGTTAAGTCATTCGAAAGAGCGTTTAATAAGCAATCTGGATGTATGGACGAGAATGTGAGCTGCGAAAAATGGGCAAAGGCAGGGGAATGCCAGAAGAATCCAACATACATGGTTGGTTCAGACAAAGACCATGGATATTGCAGAAAGAGTTGCAAAGCTTGCTCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 map00330 map01100
Pfam Domains
Protein Families
2OG-FeII_Oxy_3 ShK

Protein Analysis

316

Amino Acids

35.57

Weight (kDa)

6.32

Isoelectric Point (pI)

39.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 141 - 260 3.9e-11 2OG-Fe(II) oxygenase superfamily
2OG-FeII_Oxy_3 PF13640 143 - 260 1.3e-18 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 76, 467
AclWI GGATC 1 cut(s) 149
AfaI GTAC 2 cut(s) 318, 539
AfiI CCNNNNNNNGG 1 cut(s) 662
AgsI TTSAA 4 cut(s) 554, 607, 685, 714
AhlI ACTAGT 1 cut(s) 323
AjnI CCWGG 1 cut(s) 489
AluBI AGCT 7 cut(s) 262, 505, 618, 720, 732, 840, 938
AluI AGCT 7 cut(s) 262, 505, 618, 720, 732, 840, 938
Alw21I GWGCWC 1 cut(s) 507
AlwI GGATC 1 cut(s) 149
ApeKI GCWGC 2 cut(s) 389, 840
Asp700I GAANNNNTTC 1 cut(s) 210
AspS9I GGNCC 2 cut(s) 396, 621
AsuHPI GGTGA 3 cut(s) 308, 431, 683
AsuII TTCGAA 1 cut(s) 792
AvaII GGWCC 2 cut(s) 396, 621
BanII GRGCYC 1 cut(s) 507
BauI CACGAG 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 507
BbvI GCAGC 2 cut(s) 376, 827
BccI CCATC 2 cut(s) 101, 765
BciT130I CCWGG 1 cut(s) 491
BclI TGATCA 1 cut(s) 232
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 5 cut(s) 87, 102, 189, 324, 500
BisI GCNGC 2 cut(s) 390, 841
BlsI GCNGC 2 cut(s) 391, 842
Bme1390I CCNGG 1 cut(s) 491
Bme18I GGWCC 2 cut(s) 396, 621
BmgT120I GGNCC 2 cut(s) 396, 621
BmrFI CCNGG 1 cut(s) 491
BmsI GCATC 1 cut(s) 658
Bpu14I TTCGAA 1 cut(s) 792
BpuEI CTTGAG 1 cut(s) 284
BsaJI CCNNGG 2 cut(s) 656, 910
BsaXI ACNNNNNCTCC 2 cut(s) 555, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 662
Bse1I ACTGG 1 cut(s) 737
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 2 cut(s) 656, 910
BseGI GGATG 3 cut(s) 361, 673, 824
BseLI CCNNNNNNNGG 1 cut(s) 662
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 1 cut(s) 737
BseRI GAGGAG 1 cut(s) 576
BseXI GCAGC 2 cut(s) 376, 827
BsgI GTGCAG 1 cut(s) 51
BsiHKAI GWGCWC 1 cut(s) 507
BslI CCNNNNNNNGG 1 cut(s) 662
BsmI GAATGC 1 cut(s) 872
Bsp119I TTCGAA 1 cut(s) 792
Bsp1286I GDGCHC 1 cut(s) 507
Bsp143I GATC 3 cut(s) 89, 154, 232
Bsp19I CCATGG 1 cut(s) 910
BspACI CCGC 2 cut(s) 76, 467
BspCNI CTCAG 1 cut(s) 613
BspPI GGATC 1 cut(s) 149
BspT104I TTCGAA 1 cut(s) 792
BsrI ACTGG 1 cut(s) 737
BssECI CCNNGG 2 cut(s) 656, 910
BssMI GATC 3 cut(s) 89, 154, 232
BssSI CACGAG 1 cut(s) 162
BssT1I CCWWGG 1 cut(s) 910
Bst2BI CACGAG 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 491
Bst4CI ACNGT 3 cut(s) 529, 571, 678
Bst6I CTCTTC 2 cut(s) 47, 405
BstAPI GCANNNNNTGC 3 cut(s) 386, 436, 939
BstBI TTCGAA 1 cut(s) 792
BstC8I GCNNGC 1 cut(s) 940
BstDEI CTNAG 1 cut(s) 600
BstDSI CCRYGG 1 cut(s) 910
BstF5I GGATG 3 cut(s) 361, 673, 824
BstKTI GATC 3 cut(s) 92, 157, 235
BstMBI GATC 3 cut(s) 89, 154, 232
BstMWI GCNNNNNNNGC 3 cut(s) 386, 436, 939
BstNI CCWGG 1 cut(s) 491
BstSCI CCNGG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 376, 827
BtgI CCRYGG 1 cut(s) 910
BtsCI GGATG 3 cut(s) 361, 673, 824
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 4 cut(s) 120, 576, 654, 744
Cac8I GCNNGC 1 cut(s) 940
Cfr13I GGNCC 2 cut(s) 396, 621
Csp6I GTAC 2 cut(s) 317, 538
CviAII CATG 5 cut(s) 427, 580, 725, 889, 911
CviQI GTAC 2 cut(s) 317, 538
DdeI CTNAG 1 cut(s) 600
DpnI GATC 3 cut(s) 91, 156, 234
DpnII GATC 3 cut(s) 89, 154, 232
Eam1104I CTCTTC 2 cut(s) 47, 405
EarI CTCTTC 2 cut(s) 47, 405
Ecl136II GAGCTC 1 cut(s) 505
Eco130I CCWWGG 1 cut(s) 910
Eco24I GRGCYC 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 396, 621
Eco53kI GAGCTC 1 cut(s) 505
EcoICRI GAGCTC 1 cut(s) 505
EcoRII CCWGG 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 910
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 910
FaeI CATG 5 cut(s) 430, 583, 728, 892, 914
FalI AAGNNNNNCTT 6 cut(s) 124, 156, 198, 230, 246, 278
FatI CATG 5 cut(s) 426, 579, 724, 888, 910
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 2 cut(s) 390, 841
FokI GGATG 3 cut(s) 368, 680, 831
FriOI GRGCYC 1 cut(s) 507
Fsp4HI GCNGC 2 cut(s) 390, 841
FspBI CTAG 5 cut(s) 87, 102, 189, 324, 500
GluI GCNGC 2 cut(s) 390, 841
Hin1II CATG 5 cut(s) 430, 583, 728, 892, 914
HindIII AAGCTT 2 cut(s) 260, 936
HinfI GANTC 7 cut(s) 5, 184, 290, 422, 710, 775, 877
HphI GGTGA 3 cut(s) 308, 431, 683
Hpy166II GTNNAC 1 cut(s) 396
Hpy188I TCNGA 4 cut(s) 12, 220, 519, 901
Hpy188III TCNNGA 5 cut(s) 330, 407, 485, 500, 816
Hpy8I GTNNAC 1 cut(s) 396
HpyAV CCTTC 2 cut(s) 200, 580
HpyCH4III ACNGT 3 cut(s) 529, 571, 678
HpyCH4IV ACGT 1 cut(s) 780
HpyCH4V TGCA 9 cut(s) 68, 430, 439, 632, 647, 671, 701, 921, 933
HpyF10VI GCNNNNNNNGC 3 cut(s) 386, 436, 939
HpyF3I CTNAG 1 cut(s) 600
HpySE526I ACGT 1 cut(s) 780
Hsp92II CATG 5 cut(s) 430, 583, 728, 892, 914
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 3 cut(s) 89, 154, 232
Lsp1109I GCAGC 2 cut(s) 376, 827
LweI GCATC 1 cut(s) 658
MaeI CTAG 5 cut(s) 87, 102, 189, 324, 500
MaeII ACGT 1 cut(s) 780
MaeIII GTNAC 2 cut(s) 95, 166
MalI GATC 3 cut(s) 91, 156, 234
MboI GATC 3 cut(s) 89, 154, 232
MboII GAAGA 7 cut(s) 34, 132, 395, 422, 673, 680, 886
MhlI GDGCHC 1 cut(s) 507
MluCI AATT 2 cut(s) 57, 245
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 2 cut(s) 182, 905
MnlI CCTC 8 cut(s) 41, 63, 92, 217, 370, 554, 651, 739
Mph1103I ATGCAT 1 cut(s) 673
MroXI GAANNNNTTC 1 cut(s) 210
MseI TTAA 3 cut(s) 783, 804, 949
MslI CAYNNNNRTG 1 cut(s) 696
MspR9I CCNGG 1 cut(s) 491
Mva1269I GAATGC 1 cut(s) 872
MvaI CCWGG 1 cut(s) 491
MwoI GCNNNNNNNGC 3 cut(s) 386, 436, 939
NcoI CCATGG 1 cut(s) 910
NdeII GATC 3 cut(s) 89, 154, 232
NlaIII CATG 5 cut(s) 430, 583, 728, 892, 914
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 1 cut(s) 673
NspV TTCGAA 1 cut(s) 792
PctI GAATGC 1 cut(s) 872
PdmI GAANNNNTTC 1 cut(s) 210
PfeI GAWTC 6 cut(s) 5, 290, 422, 710, 775, 877
PkrI GCNGC 2 cut(s) 391, 842
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 507
Psp6I CCWGG 1 cut(s) 489
PspGI CCWGG 1 cut(s) 489
PspPI GGNCC 2 cut(s) 396, 621
RsaI GTAC 2 cut(s) 318, 539
RsaNI GTAC 2 cut(s) 317, 538
RseI CAYNNNNRTG 1 cut(s) 696
SacI GAGCTC 1 cut(s) 507
SaqAI TTAA 3 cut(s) 783, 804, 949
SatI GCNGC 2 cut(s) 390, 841
Sau3AI GATC 3 cut(s) 89, 154, 232
Sau96I GGNCC 2 cut(s) 396, 621
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 1 cut(s) 491
SduI GDGCHC 1 cut(s) 507
SfaNI GCATC 1 cut(s) 658
SfuI TTCGAA 1 cut(s) 792
SinI GGWCC 2 cut(s) 396, 621
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 263
SmoI CTYRAG 1 cut(s) 263
SpeI ACTAGT 1 cut(s) 323
Sse9I AATT 2 cut(s) 57, 245
SsiI CCGC 2 cut(s) 76, 467
SspI AATATT 1 cut(s) 435
SspMI CTAG 5 cut(s) 87, 102, 189, 324, 500
SstI GAGCTC 1 cut(s) 507
StyD4I CCNGG 1 cut(s) 489
StyI CCWWGG 1 cut(s) 910
TaaI ACNGT 3 cut(s) 529, 571, 678
TaiI ACGT 1 cut(s) 783
TaqI TCGA 2 cut(s) 92, 792
TaqII GACCGA 1 cut(s) 638
TasI AATT 2 cut(s) 57, 245
TatI WGTACW 1 cut(s) 537
TfiI GAWTC 6 cut(s) 5, 290, 422, 710, 775, 877
Tru1I TTAA 3 cut(s) 783, 804, 949
Tru9I TTAA 3 cut(s) 783, 804, 949
TscAI CASTG 4 cut(s) 120, 576, 654, 744
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 389, 840
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 4 cut(s) 51, 140, 219, 477
TspRI CASTG 4 cut(s) 120, 576, 654, 744
VpaK11BI GGWCC 2 cut(s) 396, 621
XbaI TCTAGA 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 210
XspI CTAG 5 cut(s) 87, 102, 189, 324, 500
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.