AT3G29780

ralf-like 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008229
Physical Location & Seq
Forward (+)
11671637 .. 11672239
603 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G29780.1

Sequence Viewer

Length: 354 bp
ATGACCAAAACATTTTTCTCCTTCTCCTTCTTCTTCACCAGCTCTCTTCTCCTTCTCCTCGCGGCAACTTCCGCAACCGCATCTACAGGGAATGTTACTAGTGGGTTGAGATATGACGGATGTGCTCCTGGAGACACCGTTGGAGAATGTATAACCGCAACGGTTGAGGAGGAGGATGAGGAAGGAGTTGAGGCGGTAGTCCGGCGGATTCTACAACAAAGAAAGTACTTAAGCTACAAAACTTTGCAGAAGCAGCCAACTTGTGATGGTAGAATTGCTGGTAATTGCATAGGGACTGTTAATCCAAAAGGTGCAACTTGTACTTACTACCAGAGATGCAAACGTGCCGCATAG

Protein Analysis

117

Amino Acids

12.7

Weight (kDa)

8.23

Isoelectric Point (pI)

48.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 61 - 115 3.3e-12 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 62
AciI CCGC 7 cut(s) 62, 72, 78, 156, 194, 205, 348
AfaI GTAC 2 cut(s) 227, 322
AflII CTTAAG 1 cut(s) 229
AhlI ACTAGT 1 cut(s) 98
AjnI CCWGG 1 cut(s) 127
AjuI GAANNNNNNNTTGG 1 cut(s) 31
AluBI AGCT 2 cut(s) 42, 234
AluI AGCT 2 cut(s) 42, 234
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 126
ApeKI GCWGC 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 265
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 126
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 229
BisI GCNGC 3 cut(s) 63, 254, 348
BlsI GCNGC 3 cut(s) 64, 255, 349
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 129
BmrFI CCNGG 1 cut(s) 129
BmsI GCATC 2 cut(s) 89, 326
BpmI CTGGAG 1 cut(s) 150
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
BseBI CCWGG 1 cut(s) 129
BseGI GGATG 2 cut(s) 125, 181
BseRI GAGGAG 3 cut(s) 47, 182, 185
BseXI GCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 62
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 127
BspACI CCGC 7 cut(s) 62, 72, 78, 156, 194, 205, 348
BspFNI CGCG 1 cut(s) 62
BspTI CTTAAG 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 129
Bst4CI ACNGT 3 cut(s) 139, 163, 298
Bst6I CTCTTC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 229
BstF5I GGATG 2 cut(s) 125, 181
BstFNI CGCG 1 cut(s) 62
BstMAI GTCTC 1 cut(s) 126
BstMWI GCNNNNNNNGC 2 cut(s) 71, 253
BstNI CCWGG 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 127
BstSFI CTRYAG 1 cut(s) 84
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 265
BtsCI GGATG 2 cut(s) 125, 181
Csp6I GTAC 2 cut(s) 226, 321
CviJI RGCY 3 cut(s) 42, 234, 256
CviKI_1 RGCY 3 cut(s) 42, 234, 256
CviQI GTAC 2 cut(s) 226, 321
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
EciI GGCGGA 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 127
FaiI YATR 4 cut(s) 114, 152, 290, 352
FaqI GGGAC 1 cut(s) 307
Fnu4HI GCNGC 3 cut(s) 63, 254, 348
FokI GGATG 2 cut(s) 132, 188
Fsp4HI GCNGC 3 cut(s) 63, 254, 348
FspBI CTAG 1 cut(s) 99
GluI GCNGC 3 cut(s) 63, 254, 348
GsuI CTGGAG 1 cut(s) 150
HapII CCGG 1 cut(s) 202
HinfI GANTC 1 cut(s) 208
HpaII CCGG 1 cut(s) 202
HphI GGTGA 1 cut(s) 28
HpyAV CCTTC 4 cut(s) 31, 37, 62, 176
HpyCH4III ACNGT 3 cut(s) 139, 163, 298
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 4 cut(s) 247, 288, 314, 339
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 253
HpySE526I ACGT 1 cut(s) 343
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 7 cut(s) 52, 72, 114, 141, 215, 264, 344
Lsp1109I GCAGC 1 cut(s) 265
LweI GCATC 2 cut(s) 89, 326
MaeI CTAG 1 cut(s) 99
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 1 cut(s) 94
MboII GAAGA 3 cut(s) 22, 25, 38
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 2 cut(s) 273, 283
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 6 cut(s) 68, 160, 163, 166, 172, 184
MseI TTAA 2 cut(s) 230, 300
MspCI CTTAAG 1 cut(s) 229
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 1 cut(s) 129
MvaI CCWGG 1 cut(s) 129
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 2 cut(s) 71, 253
PfeI GAWTC 1 cut(s) 208
PfoI TCCNGGA 1 cut(s) 127
PkrI GCNGC 3 cut(s) 64, 255, 349
Psp6I CCWGG 1 cut(s) 127
PspGI CCWGG 1 cut(s) 127
RsaI GTAC 2 cut(s) 227, 322
RsaNI GTAC 2 cut(s) 226, 321
SaqAI TTAA 2 cut(s) 230, 300
SatI GCNGC 3 cut(s) 63, 254, 348
ScaI AGTACT 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 129
SduI GDGCHC 1 cut(s) 127
SetI ASST 4 cut(s) 44, 236, 313, 346
SfaNI GCATC 2 cut(s) 89, 326
SfcI CTRYAG 1 cut(s) 84
SmlI CTYRAG 1 cut(s) 229
SmoI CTYRAG 1 cut(s) 229
SpeI ACTAGT 1 cut(s) 98
Sse9I AATT 2 cut(s) 273, 283
SsiI CCGC 7 cut(s) 62, 72, 78, 156, 194, 205, 348
SspMI CTAG 1 cut(s) 99
StyD4I CCNGG 1 cut(s) 127
TaaI ACNGT 3 cut(s) 139, 163, 298
TaiI ACGT 1 cut(s) 346
TasI AATT 2 cut(s) 273, 283
TatI WGTACW 2 cut(s) 225, 320
TauI GCSGC 2 cut(s) 65, 350
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 2 cut(s) 230, 300
Tru9I TTAA 2 cut(s) 230, 300
TseI GCWGC 1 cut(s) 253
TspGWI ACGGA 1 cut(s) 132
Vha464I CTTAAG 1 cut(s) 229
XspI CTAG 1 cut(s) 99
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.