RchiOBHm_Chr6g0275191

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008229
Physical Location & Seq
Forward (+)
36830349 .. 36830815
467 bp
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UTR
Exon/CDS
Intron
PRQ24688

Sequence Viewer

Length: 423 bp
ATGGTGATGATAGACATGAATACATTTACAGCAATAAATTTTTTGCTTGTGATGACCTTGGCAATGACAATGATAGCTGACTCGTCATCAAACCCAAATCATTTCTCAACCGACATGCGGAAAGTTGGAGCAGTGCCTGCACCAAAACGTCGAGATAATAACAATTTCTCATCATGTAACGGCTTCTTTGGCGACTGCCACAGTTATTCTGATGAGGATTTTATGACTAGTGAAAGAAGAAGGACAAGAAGAAGACATCTTTATGAAGCCAGAAGGTATATAAGTTATAGAGCCCTACTGAAAGATCGTGTTCCCTGTGGTCAACGAGGCAGCTCATATTACAATTGTGGTATTTCTAGGCAAGCTAATCCTTACAGGCGTGGTTGTAGTGTCATCACACATTGTGCCAGAAGTAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

140

Amino Acids

16.1

Weight (kDa)

9.64

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 82 - 137 1.2e-21 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 118
AcsI RAATTY 1 cut(s) 37
AdeI CACNNNGTG 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 117
AhlI ACTAGT 1 cut(s) 227
AluBI AGCT 3 cut(s) 77, 333, 365
AluI AGCT 3 cut(s) 77, 333, 365
AlwNI CAGNNNCTG 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 330
ApoI RAATTY 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 16
BanII GRGCYC 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 342
BceAI ACGGC 1 cut(s) 196
BcuI ACTAGT 1 cut(s) 227
BfaI CTAG 2 cut(s) 228, 357
BisI GCNGC 1 cut(s) 331
BlsI GCNGC 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 259
BsaJI CCNNGG 1 cut(s) 57
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 69
BseDI CCNNGG 1 cut(s) 57
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMI GCAATG 1 cut(s) 69
BseXI GCAGC 1 cut(s) 342
BsgI GTGCAG 1 cut(s) 123
BslI CCNNNNNNNGG 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 1 cut(s) 304
BspACI CCGC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 69
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 1 cut(s) 304
BssT1I CCWWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 203
BstAPI GCANNNNNTGC 1 cut(s) 137
BstC8I GCNNGC 2 cut(s) 138, 363
BstKTI GATC 1 cut(s) 307
BstMBI GATC 1 cut(s) 304
BstMWI GCNNNNNNNGC 2 cut(s) 137, 189
BstNSI RCATGY 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 342
BstV2I GAAGAC 1 cut(s) 259
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 2 cut(s) 138, 363
CaiI CAGNNNCTG 1 cut(s) 137
CviAII CATG 3 cut(s) 16, 115, 174
CviJI RGCY 6 cut(s) 77, 183, 269, 293, 333, 365
CviKI_1 RGCY 6 cut(s) 77, 183, 269, 293, 333, 365
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
DraIII CACNNNGTG 1 cut(s) 404
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 1 cut(s) 295
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 3 cut(s) 19, 118, 177
FaiI YATR 9 cut(s) 17, 116, 175, 224, 264, 279, 281, 288, 337
FatI CATG 3 cut(s) 15, 114, 173
Fnu4HI GCNGC 1 cut(s) 331
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 1 cut(s) 331
FspBI CTAG 2 cut(s) 228, 357
GluI GCNGC 1 cut(s) 331
Hin1II CATG 3 cut(s) 19, 118, 177
HincII GTYRAC 1 cut(s) 323
HindII GTYRAC 1 cut(s) 323
HinfI GANTC 1 cut(s) 80
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 1 cut(s) 323
Hpy99I CGWCG 1 cut(s) 153
HpyAV CCTTC 2 cut(s) 234, 267
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 1 cut(s) 140
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 189
HpySE526I ACGT 1 cut(s) 148
Hsp92II CATG 3 cut(s) 19, 118, 177
Kzo9I GATC 1 cut(s) 304
LmnI GCTCC 1 cut(s) 128
LpnPI CCDG 4 cut(s) 150, 283, 328, 361
Lsp1109I GCAGC 1 cut(s) 342
MaeI CTAG 2 cut(s) 228, 357
MaeII ACGT 1 cut(s) 148
MaeIII GTNAC 1 cut(s) 176
MalI GATC 1 cut(s) 306
MboI GATC 1 cut(s) 304
MboII GAAGA 3 cut(s) 249, 261, 264
MfeI CAATTG 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 3 cut(s) 37, 163, 343
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 1 cut(s) 106
MnlI CCTC 2 cut(s) 208, 320
MslI CAYNNNNRTG 1 cut(s) 261
MunI CAATTG 1 cut(s) 343
MwoI GCNNNNNNNGC 2 cut(s) 137, 189
NdeII GATC 1 cut(s) 304
NlaIII CATG 3 cut(s) 19, 118, 177
NspI RCATGY 1 cut(s) 118
PkrI GCNGC 1 cut(s) 332
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
PstNI CAGNNNCTG 1 cut(s) 137
RseI CAYNNNNRTG 1 cut(s) 261
SatI GCNGC 1 cut(s) 331
Sau3AI GATC 1 cut(s) 304
SchI GAGTC 1 cut(s) 74
SduI GDGCHC 1 cut(s) 295
SetI ASST 6 cut(s) 59, 79, 151, 278, 335, 367
SmiMI CAYNNNNRTG 1 cut(s) 261
SpeI ACTAGT 1 cut(s) 227
Sse9I AATT 3 cut(s) 37, 163, 343
SsiI CCGC 1 cut(s) 118
SspMI CTAG 2 cut(s) 228, 357
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 1 cut(s) 151
TaqI TCGA 1 cut(s) 151
TasI AATT 3 cut(s) 37, 163, 343
TscAI CASTG 1 cut(s) 138
TseI GCWGC 1 cut(s) 330
TspDTI ATGAA 2 cut(s) 32, 279
TspRI CASTG 1 cut(s) 138
XapI RAATTY 1 cut(s) 37
XceI RCATGY 1 cut(s) 118
XspI CTAG 2 cut(s) 228, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.