Rw6G017640

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008229
Physical Location & Seq
Forward (+)
34963093 .. 34963509
417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017640.1

Sequence Viewer

Length: 417 bp
ATGATGGACATGAATACATTTACGGCAATACATTTTTTGCTTGTGATGGCCTTGGCAATGACAATGATAGCTGACTCGTCATCAAACCCAAATCATTTCTCAACCGACATGCGGAAAGTTGGAGCAGTGCCTGCACCAAAACGTCGAGACAATAACAATTTCTCATCATGTAACGGCTTCTTTGGCGACTGCCACAGTTATTCTGATGAGGATTTTATGACTAGTGAAAGAAGAAGGACAAGAAGAAGACATCTTTATGAAGCCAGAAGGTATATAAGTTATAGAGCCCTACTGAAAGATCGTGTTCCCTGTGGTCAACGAGGCAGCTCGTATTACAATTGTGGTATTTCTGGGCAAGCTAATCCTTATAGGCGTGGTTGTAGTGTCATCACACATTGTGCCAGAAGTAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

138

Amino Acids

15.78

Weight (kDa)

9.5

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 80 - 135 3.7e-22 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 112
AdeI CACNNNGTG 1 cut(s) 398
AfiI CCNNNNNNNGG 1 cut(s) 111
AhlI ACTAGT 1 cut(s) 221
AluBI AGCT 3 cut(s) 71, 327, 359
AluI AGCT 3 cut(s) 71, 327, 359
Alw26I GTCTC 1 cut(s) 141
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 324
BanII GRGCYC 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 253
BbvI GCAGC 1 cut(s) 336
BccI CCATC 1 cut(s) 40
BceAI ACGGC 2 cut(s) 39, 190
BcoDI GTCTC 1 cut(s) 141
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 1 cut(s) 222
BisI GCNGC 1 cut(s) 325
BlsI GCNGC 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 51
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse3DI GCAATG 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 63
BseXI GCAGC 1 cut(s) 336
BsgI GTGCAG 1 cut(s) 117
BshFI GGCC 1 cut(s) 50
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 141
BsnI GGCC 1 cut(s) 50
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 1 cut(s) 298
BspACI CCGC 1 cut(s) 112
BspANI GGCC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 63
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 197
BstAPI GCANNNNNTGC 1 cut(s) 131
BstC8I GCNNGC 2 cut(s) 132, 357
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 1 cut(s) 298
BstMWI GCNNNNNNNGC 2 cut(s) 131, 183
BstNSI RCATGY 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 336
BstV2I GAAGAC 1 cut(s) 253
BsuRI GGCC 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 2 cut(s) 132, 357
CaiI CAGNNNCTG 1 cut(s) 131
CviAII CATG 3 cut(s) 10, 109, 168
CviJI RGCY 7 cut(s) 50, 71, 177, 263, 287, 327, 359
CviKI_1 RGCY 7 cut(s) 50, 71, 177, 263, 287, 327, 359
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
DraIII CACNNNGTG 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 3 cut(s) 13, 112, 171
FaiI YATR 9 cut(s) 11, 110, 169, 218, 258, 273, 275, 282, 369
FatI CATG 3 cut(s) 9, 108, 167
Fnu4HI GCNGC 1 cut(s) 325
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 1 cut(s) 325
FspBI CTAG 1 cut(s) 222
GluI GCNGC 1 cut(s) 325
HaeIII GGCC 1 cut(s) 50
Hin1II CATG 3 cut(s) 13, 112, 171
HincII GTYRAC 1 cut(s) 317
HindII GTYRAC 1 cut(s) 317
HinfI GANTC 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 1 cut(s) 146
Hpy8I GTNNAC 1 cut(s) 317
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 2 cut(s) 228, 261
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4IV ACGT 1 cut(s) 142
HpyCH4V TGCA 1 cut(s) 134
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 183
HpySE526I ACGT 1 cut(s) 142
Hsp92II CATG 3 cut(s) 13, 112, 171
Kzo9I GATC 1 cut(s) 298
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 4 cut(s) 144, 277, 322, 336
Lsp1109I GCAGC 1 cut(s) 336
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 142
MaeIII GTNAC 1 cut(s) 170
MalI GATC 1 cut(s) 300
MboI GATC 1 cut(s) 298
MboII GAAGA 3 cut(s) 243, 255, 258
MfeI CAATTG 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 2 cut(s) 157, 337
MlyI GAGTC 1 cut(s) 68
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 2 cut(s) 202, 314
MslI CAYNNNNRTG 1 cut(s) 255
MunI CAATTG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 131, 183
NdeII GATC 1 cut(s) 298
NlaIII CATG 3 cut(s) 13, 112, 171
NspI RCATGY 1 cut(s) 112
PkrI GCNGC 1 cut(s) 326
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PstNI CAGNNNCTG 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 255
SatI GCNGC 1 cut(s) 325
Sau3AI GATC 1 cut(s) 298
SchI GAGTC 1 cut(s) 68
SduI GDGCHC 1 cut(s) 289
SetI ASST 5 cut(s) 73, 145, 272, 329, 361
SmiMI CAYNNNNRTG 1 cut(s) 255
SpeI ACTAGT 1 cut(s) 221
Sse9I AATT 2 cut(s) 157, 337
SsiI CCGC 1 cut(s) 112
SspMI CTAG 1 cut(s) 222
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 197
TaiI ACGT 1 cut(s) 145
TaqI TCGA 1 cut(s) 145
TasI AATT 2 cut(s) 157, 337
TscAI CASTG 1 cut(s) 132
TseI GCWGC 1 cut(s) 324
TspDTI ATGAA 2 cut(s) 26, 273
TspRI CASTG 1 cut(s) 132
XceI RCATGY 1 cut(s) 112
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.