FvH4_2g13590

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008229
Physical Location & Seq
Forward (+)
11869901 .. 11870362
462 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13590.t1

Sequence Viewer

Length: 462 bp
ATGGAGATCAATACATTTACACTAGTAAAATTTCTCTGCGTGATATCTTTGGCAGTTACAGTAATGGTCGTAGACTCATTATCAACCCCACAACATTACTCAGTTAGAACCAGCAATCATGGAGGCGCAATGTCAAGTACTACTGCACCAGAACTTCTAGATATTAAAAATATCGCATCTTGCAGCAAAGGCTTCTCCGGTGACTGCCAGAATTATTCTGTTGAAAATGAAGAAGATGGTGTTAATGGTTTCTTTGATGACTTCAGTGACTATTCTGATGAGGATATTGTGACTAGCCAAAGAAGAAGAACCAAAAGGTATATAAGCTATGATGCCCTCCTGAAAAATCGTGTTCCTTGTGGTCAACGAGGCAGTTCGTATTACTATTGTGGTATTCCTGGGCAGGCTAATCCTTATAGGCGTGGTTGTAGTGTCATCACACGTTGTGCTAGATTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

154

Amino Acids

17.05

Weight (kDa)

6.7

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 102 - 151 4.6e-21 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 72
AcsI RAATTY 1 cut(s) 29
AcuI CTGAAG 1 cut(s) 247
AdeI CACNNNGTG 1 cut(s) 446
AfaI GTAC 1 cut(s) 139
AflIII ACRYGT 1 cut(s) 440
AgsI TTSAA 1 cut(s) 224
AhlI ACTAGT 1 cut(s) 22
AjnI CCWGG 1 cut(s) 397
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 183
ApoI RAATTY 1 cut(s) 29
AspLEI GCGC 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 212
BbvI GCAGC 1 cut(s) 195
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 399
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 4 cut(s) 23, 158, 294, 450
BisI GCNGC 1 cut(s) 184
BlsI GCNGC 1 cut(s) 185
BmcAI AGTACT 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 399
BmrFI CCNGG 1 cut(s) 399
BmsI GCATC 2 cut(s) 185, 322
BsaJI CCNNGG 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 197
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 399
BseDI CCNNGG 1 cut(s) 398
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 1 cut(s) 114
BseXI GCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 129
BsiSI CCGG 1 cut(s) 198
Bsp143I GATC 1 cut(s) 6
BspCNI CTCAG 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 135
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 399
Bst4CI ACNGT 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 1 cut(s) 100
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 1 cut(s) 399
BstSCI CCNGG 1 cut(s) 397
BstV1I GCAGC 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 405
CfoI GCGC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 138
CviAII CATG 1 cut(s) 119
CviJI RGCY 4 cut(s) 192, 297, 327, 407
CviKI_1 RGCY 4 cut(s) 192, 297, 327, 407
CviQI GTAC 1 cut(s) 138
DdeI CTNAG 1 cut(s) 100
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
DraIII CACNNNGTG 1 cut(s) 446
Eco32I GATATC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 397
EcoRV GATATC 1 cut(s) 45
FaeI CATG 1 cut(s) 122
FaiI YATR 5 cut(s) 120, 321, 323, 330, 417
FatI CATG 1 cut(s) 118
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 184
FspBI CTAG 4 cut(s) 23, 158, 294, 450
GlaI GCGC 1 cut(s) 127
GluI GCNGC 1 cut(s) 184
HapII CCGG 1 cut(s) 198
HhaI GCGC 1 cut(s) 128
Hin1II CATG 1 cut(s) 122
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 365
HindII GTYRAC 1 cut(s) 365
HinfI GANTC 1 cut(s) 74
HpaII CCGG 1 cut(s) 198
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 2 cut(s) 73, 365
Hpy188I TCNGA 1 cut(s) 277
Hpy188III TCNNGA 2 cut(s) 158, 340
Hpy8I GTNNAC 2 cut(s) 73, 365
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4IV ACGT 1 cut(s) 442
HpyCH4V TGCA 2 cut(s) 146, 183
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpyF3I CTNAG 1 cut(s) 100
HpySE526I ACGT 1 cut(s) 442
Hsp92II CATG 1 cut(s) 122
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 1 cut(s) 6
LpnPI CCDG 8 cut(s) 124, 162, 211, 221, 353, 384, 389, 411
Lsp1109I GCAGC 1 cut(s) 195
LweI GCATC 2 cut(s) 185, 322
MaeI CTAG 4 cut(s) 23, 158, 294, 450
MaeII ACGT 1 cut(s) 442
MaeIII GTNAC 4 cut(s) 55, 200, 266, 289
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 4 cut(s) 242, 245, 315, 318
MluCI AATT 3 cut(s) 29, 211, 457
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 4 cut(s) 116, 274, 347, 362
MseI TTAA 4 cut(s) 165, 243, 456, 460
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 1 cut(s) 399
MvaI CCWGG 1 cut(s) 399
MwoI GCNNNNNNNGC 1 cut(s) 189
NdeII GATC 1 cut(s) 6
NlaIII CATG 1 cut(s) 122
NmuCI GTSAC 3 cut(s) 200, 266, 289
PacI TTAATTAA 1 cut(s) 460
PkrI GCNGC 1 cut(s) 185
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
Psp6I CCWGG 1 cut(s) 397
PspGI CCWGG 1 cut(s) 397
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
SaqAI TTAA 4 cut(s) 165, 243, 456, 460
SatI GCNGC 1 cut(s) 184
Sau3AI GATC 1 cut(s) 6
ScaI AGTACT 1 cut(s) 139
SchI GAGTC 1 cut(s) 68
ScrFI CCNGG 1 cut(s) 399
SetI ASST 3 cut(s) 320, 329, 445
SfaNI GCATC 2 cut(s) 185, 322
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 3 cut(s) 29, 211, 457
SspMI CTAG 4 cut(s) 23, 158, 294, 450
StyD4I CCNGG 1 cut(s) 397
TaaI ACNGT 1 cut(s) 61
TaiI ACGT 1 cut(s) 445
TasI AATT 3 cut(s) 29, 211, 457
TatI WGTACW 1 cut(s) 137
Tru1I TTAA 4 cut(s) 165, 243, 456, 460
Tru9I TTAA 4 cut(s) 165, 243, 456, 460
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 3 cut(s) 200, 266, 289
TseI GCWGC 1 cut(s) 183
Tsp45I GTSAC 3 cut(s) 200, 266, 289
TspDTI ATGAA 1 cut(s) 243
TspRI CASTG 1 cut(s) 271
XapI RAATTY 1 cut(s) 29
XbaI TCTAGA 1 cut(s) 157
XmiI GTMKAC 1 cut(s) 72
XspI CTAG 4 cut(s) 23, 158, 294, 450
ZrmI AGTACT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.