Rh6DG198600

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008229
Physical Location & Seq
Forward (+)
35461600 .. 35462004
405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG198600.1

Sequence Viewer

Length: 405 bp
ATGGAGATCAATACATTTAGATTAGTAAATTTCCTTTGTGTGACATCTTTGGCAATGGGAATGGTGGTTGACTCAATATCATCAACCCCACAACACTTCTCTGTTAGAACCAGCAATCATGGAAGGGCAATGTCAACTGCACCACCGCGTCCAGATATTAAGAATATCGATTCTTGCAGTGATGGCTTTTCTGGTGACTGCCTCAGTTATTCTGTTGAAGATGAAGAAGATGGTTCTAATGATGATAGGCCTACAAGAACTATAAGCTATGAAGCCCTAAAAAGAAATATGGTTCCATGTGGTCAACGTGGACATTCGTATTACTATTGTGGTATATCTGGGCAGGCTAATCCTTATAGGCGTGGCTGCAGTATCATCACACGTTGTGCTAGATCTACTGATTAA

Protein Analysis

134

Amino Acids

14.8

Weight (kDa)

6.27

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 86 - 131 1.2e-18 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 148
AciI CCGC 1 cut(s) 146
AcsI RAATTY 1 cut(s) 28
AdeI CACNNNGTG 1 cut(s) 386
AflIII ACRYGT 1 cut(s) 380
AgsI TTSAA 1 cut(s) 218
AluBI AGCT 1 cut(s) 267
AluI AGCT 1 cut(s) 267
AoxI GGCC 1 cut(s) 248
ApeKI GCWGC 1 cut(s) 366
ApoI RAATTY 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 206
BbvI GCAGC 1 cut(s) 353
BccI CCATC 2 cut(s) 176, 224
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 1 cut(s) 367
BglII AGATCT 1 cut(s) 392
BisI GCNGC 1 cut(s) 367
BlsI GCNGC 1 cut(s) 368
BmiI GGNNCC 1 cut(s) 294
Bsa29I ATCGAT 1 cut(s) 168
Bse3DI GCAATG 2 cut(s) 60, 135
BseCI ATCGAT 1 cut(s) 168
BseMI GCAATG 2 cut(s) 60, 135
BseMII CTCAG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 148
BshFI GGCC 1 cut(s) 250
BshVI ATCGAT 1 cut(s) 168
BsnI GGCC 1 cut(s) 250
Bsp143I GATC 2 cut(s) 6, 392
BspACI CCGC 1 cut(s) 146
BspANI GGCC 1 cut(s) 250
BspCNI CTCAG 1 cut(s) 216
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 148
BspLI GGNNCC 1 cut(s) 294
BspMAI CTGCAG 1 cut(s) 371
BsrDI GCAATG 2 cut(s) 60, 135
BssMI GATC 2 cut(s) 6, 392
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 1 cut(s) 203
BstFNI CGCG 1 cut(s) 148
BstKTI GATC 2 cut(s) 9, 395
BstMBI GATC 2 cut(s) 6, 392
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstSFI CTRYAG 1 cut(s) 367
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 1 cut(s) 353
BstX2I RGATCY 1 cut(s) 392
BstYI RGATCY 1 cut(s) 392
Bsu15I ATCGAT 1 cut(s) 168
BsuRI GGCC 1 cut(s) 250
BsuTUI ATCGAT 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 184
BtsIMutI CAGTG 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 1 cut(s) 137
CviAII CATG 2 cut(s) 119, 297
CviJI RGCY 6 cut(s) 186, 250, 267, 275, 347, 366
CviKI_1 RGCY 6 cut(s) 186, 250, 267, 275, 347, 366
DdeI CTNAG 1 cut(s) 203
DpnI GATC 2 cut(s) 8, 394
DpnII GATC 2 cut(s) 6, 392
DraIII CACNNNGTG 1 cut(s) 386
Eco147I AGGCCT 1 cut(s) 250
FaeI CATG 2 cut(s) 122, 300
FaiI YATR 7 cut(s) 120, 263, 270, 290, 298, 335, 357
FatI CATG 2 cut(s) 118, 296
Fnu4HI GCNGC 1 cut(s) 367
Fsp4HI GCNGC 1 cut(s) 367
FspBI CTAG 1 cut(s) 390
GluI GCNGC 1 cut(s) 367
HaeIII GGCC 1 cut(s) 250
HgaI GACGC 1 cut(s) 137
Hin1II CATG 2 cut(s) 122, 300
HincII GTYRAC 3 cut(s) 70, 135, 305
HindII GTYRAC 3 cut(s) 70, 135, 305
HinfI GANTC 2 cut(s) 71, 170
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 4 cut(s) 70, 135, 305, 311
Hpy188III TCNNGA 1 cut(s) 152
Hpy8I GTNNAC 4 cut(s) 70, 135, 305, 311
HpyAV CCTTC 1 cut(s) 117
HpyCH4IV ACGT 2 cut(s) 307, 382
HpyCH4V TGCA 3 cut(s) 140, 177, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 1 cut(s) 203
HpySE526I ACGT 2 cut(s) 307, 382
Hsp92II CATG 2 cut(s) 122, 300
Kzo9I GATC 2 cut(s) 6, 392
LpnPI CCDG 5 cut(s) 124, 165, 177, 324, 329
Lsp1109I GCAGC 1 cut(s) 353
MaeI CTAG 1 cut(s) 390
MaeII ACGT 2 cut(s) 307, 382
MaeIII GTNAC 2 cut(s) 40, 194
MalI GATC 2 cut(s) 8, 394
MboI GATC 2 cut(s) 6, 392
MboII GAAGA 3 cut(s) 230, 236, 239
MflI RGATCY 1 cut(s) 392
MluCI AATT 1 cut(s) 28
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 1 cut(s) 212
MseI TTAA 2 cut(s) 159, 403
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 1 cut(s) 183
NdeII GATC 2 cut(s) 6, 392
NlaIII CATG 2 cut(s) 122, 300
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 2 cut(s) 40, 194
PceI AGGCCT 1 cut(s) 250
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 368
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
PspN4I GGNNCC 1 cut(s) 294
PstI CTGCAG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 392
SaqAI TTAA 2 cut(s) 159, 403
SatI GCNGC 1 cut(s) 367
Sau3AI GATC 2 cut(s) 6, 392
SchI GAGTC 1 cut(s) 65
SetI ASST 3 cut(s) 269, 310, 385
SfcI CTRYAG 1 cut(s) 367
Sse9I AATT 1 cut(s) 28
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 1 cut(s) 146
SspMI CTAG 1 cut(s) 390
StuI AGGCCT 1 cut(s) 250
TaiI ACGT 2 cut(s) 310, 385
TaqI TCGA 1 cut(s) 168
TasI AATT 1 cut(s) 28
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 159, 403
Tru9I TTAA 2 cut(s) 159, 403
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 2 cut(s) 40, 194
TseI GCWGC 1 cut(s) 366
Tsp45I GTSAC 2 cut(s) 40, 194
TspDTI ATGAA 2 cut(s) 237, 285
TspRI CASTG 1 cut(s) 184
XapI RAATTY 1 cut(s) 28
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.