RLG00000013488

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008229
Physical Location & Seq
Reverse (-)
32278935 .. 32279282
348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013488

Sequence Viewer

Length: 348 bp
ATGGGAATGGTGGTTGACTCAATATCATCAACCCCACAACATTTCTCAGTTAGAACCAGCAATCATGGAAGGGCAATGTCAACTGCACCACCGCGTCCAGATATTAAGAATATCGATTCTTGCAGCGATGGCTTTTCTGGTGACTGCCTCAGTTATTCTGTTGAAGATGAAGAAGATGGTTCTAATGATGATAGGCCTAGATATATAAGCTATGAAGCCCTAAAAAAAAATATGGTTCCATGTGGTCAACCCGGACATTCGTATTACTCTTGCGGTAGATCTGGGCAGGCTAATCCTTATAGGCGTGGCTGCAGTGTCATCACACGTTGTGCTAGATTTATTGATTGA

Protein Analysis

116

Amino Acids

12.65

Weight (kDa)

6.26

Isoelectric Point (pI)

46.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 65 - 112 1.6e-21 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 94
AciI CCGC 2 cut(s) 92, 273
AdeI CACNNNGTG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 323
AgsI TTSAA 1 cut(s) 164
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 2 cut(s) 123, 309
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 152
BbvI GCAGC 2 cut(s) 135, 296
BccI CCATC 2 cut(s) 122, 170
BcnI CCSGG 1 cut(s) 252
BfaI CTAG 2 cut(s) 198, 333
BfmI CTRYAG 1 cut(s) 310
BglII AGATCT 1 cut(s) 278
BisI GCNGC 2 cut(s) 124, 310
BlsI GCNGC 2 cut(s) 125, 311
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 1 cut(s) 252
BpuMI CCSGG 1 cut(s) 252
Bsa29I ATCGAT 1 cut(s) 114
Bse3DI GCAATG 1 cut(s) 81
BseCI ATCGAT 1 cut(s) 114
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 2 cut(s) 60, 163
BseXI GCAGC 2 cut(s) 135, 296
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 196
BshVI ATCGAT 1 cut(s) 114
BsiSI CCGG 1 cut(s) 252
BsnI GGCC 1 cut(s) 196
Bsp143I GATC 1 cut(s) 278
BspACI CCGC 2 cut(s) 92, 273
BspANI GGCC 1 cut(s) 196
BspCNI CTCAG 2 cut(s) 59, 162
BspDI ATCGAT 1 cut(s) 114
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 237
BspMAI CTGCAG 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 81
BssMI GATC 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 288
BstDEI CTNAG 2 cut(s) 46, 149
BstFNI CGCG 1 cut(s) 94
BstKTI GATC 1 cut(s) 281
BstMBI GATC 1 cut(s) 278
BstMWI GCNNNNNNNGC 1 cut(s) 129
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 1 cut(s) 310
BstUI CGCG 1 cut(s) 94
BstV1I GCAGC 2 cut(s) 135, 296
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
Bsu15I ATCGAT 1 cut(s) 114
BsuRI GGCC 1 cut(s) 196
BsuTUI ATCGAT 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 141
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 319
Cac8I GCNNGC 1 cut(s) 288
ClaI ATCGAT 1 cut(s) 114
CseI GACGC 1 cut(s) 83
CviAII CATG 2 cut(s) 65, 240
CviJI RGCY 6 cut(s) 132, 196, 210, 218, 290, 309
CviKI_1 RGCY 6 cut(s) 132, 196, 210, 218, 290, 309
DdeI CTNAG 2 cut(s) 46, 149
DpnI GATC 1 cut(s) 280
DpnII GATC 1 cut(s) 278
DraIII CACNNNGTG 1 cut(s) 329
Eco147I AGGCCT 1 cut(s) 196
FaeI CATG 2 cut(s) 68, 243
FaiI YATR 7 cut(s) 66, 204, 206, 213, 233, 241, 300
FatI CATG 2 cut(s) 64, 239
Fnu4HI GCNGC 2 cut(s) 124, 310
Fsp4HI GCNGC 2 cut(s) 124, 310
FspBI CTAG 2 cut(s) 198, 333
GluI GCNGC 2 cut(s) 124, 310
HaeIII GGCC 1 cut(s) 196
HapII CCGG 1 cut(s) 252
HgaI GACGC 1 cut(s) 83
Hin1II CATG 2 cut(s) 68, 243
HincII GTYRAC 3 cut(s) 16, 81, 248
HindII GTYRAC 3 cut(s) 16, 81, 248
HinfI GANTC 2 cut(s) 17, 116
HpaII CCGG 1 cut(s) 252
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 3 cut(s) 16, 81, 248
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 3 cut(s) 16, 81, 248
HpyAV CCTTC 1 cut(s) 63
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 3 cut(s) 86, 123, 312
HpyF10VI GCNNNNNNNGC 1 cut(s) 129
HpyF3I CTNAG 2 cut(s) 46, 149
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 2 cut(s) 68, 243
Kzo9I GATC 1 cut(s) 278
LpnPI CCDG 6 cut(s) 70, 111, 123, 265, 267, 272
Lsp1109I GCAGC 2 cut(s) 135, 296
MaeI CTAG 2 cut(s) 198, 333
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 140
MalI GATC 1 cut(s) 280
MboI GATC 1 cut(s) 278
MboII GAAGA 3 cut(s) 176, 182, 185
MflI RGATCY 1 cut(s) 278
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 1 cut(s) 158
MseI TTAA 1 cut(s) 105
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 129
NciI CCSGG 1 cut(s) 252
NdeII GATC 1 cut(s) 278
NlaIII CATG 2 cut(s) 68, 243
NlaIV GGNNCC 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 140
PceI AGGCCT 1 cut(s) 196
PfeI GAWTC 1 cut(s) 116
PkrI GCNGC 2 cut(s) 125, 311
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 237
PstI CTGCAG 1 cut(s) 314
PsuI RGATCY 1 cut(s) 278
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 124, 310
Sau3AI GATC 1 cut(s) 278
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 252
SetI ASST 2 cut(s) 212, 328
SfcI CTRYAG 1 cut(s) 310
SseBI AGGCCT 1 cut(s) 196
SsiI CCGC 2 cut(s) 92, 273
SspMI CTAG 2 cut(s) 198, 333
StuI AGGCCT 1 cut(s) 196
StyD4I CCNGG 1 cut(s) 250
TaiI ACGT 1 cut(s) 328
TaqI TCGA 1 cut(s) 114
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 2 cut(s) 123, 309
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 183, 228
TspRI CASTG 1 cut(s) 319
XspI CTAG 2 cut(s) 198, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.