MD09G1245200.v1.1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008229
Physical Location & Seq
Forward (+)
31275564 .. 31275932
369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1245200.v1.1.491

Sequence Viewer

Length: 369 bp
ATGACGAATAATATCATTATAATTTCAGTAATTATTCTCCTTGTCATGACCTTAGCAATGGTGGTGGAGTCAACACAATACTCGAGCATGAGGCGTAACGCCGGTGAAGTAGAAGTAGCTTCACTACCGGATCGGTGTGATGGCTTGTCGGGTGAGGATTGCCTGAACATTAAGGAAGAGGAGGAGGAGGGTGACGAGGACACGGAAGAAGCTGAAACCACAAGATGCATAAGCTATGGAGCCCTAGAGAGGGATAAAGTTCCATACGATCGACGAGGCAGTTCTTATTATAATTGTGGTACATCTGGACAGGTCAATCCTTACCATCGTGGCTGCAGTGTCATTACATATTGTGCAAGGGAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

123

Amino Acids

13.54

Weight (kDa)

4.42

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 70 - 120 4.8e-17 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 20, 291
AclWI GGATC 1 cut(s) 138
AfaI GTAC 1 cut(s) 301
AfiI CCNNNNNNNGG 2 cut(s) 249, 250
AluBI AGCT 3 cut(s) 119, 212, 234
AluI AGCT 3 cut(s) 119, 212, 234
AlwI GGATC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 82
ApeKI GCWGC 1 cut(s) 333
AsuHPI GGTGA 3 cut(s) 116, 164, 203
AvaI CYCGRG 1 cut(s) 82
BanII GRGCYC 1 cut(s) 244
BbvI GCAGC 1 cut(s) 320
BccI CCATC 2 cut(s) 134, 333
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 1 cut(s) 334
BisI GCNGC 1 cut(s) 334
BlsI GCNGC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 82
BmiI GGNNCC 1 cut(s) 241
BmsI GCATC 1 cut(s) 215
Bpu10I CCTNAGC 1 cut(s) 52
BsaWI WCCGGW 1 cut(s) 127
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 250
Bse118I RCCGGY 1 cut(s) 101
Bse3DI GCAATG 1 cut(s) 63
BseLI CCNNNNNNNGG 2 cut(s) 249, 250
BseMI GCAATG 1 cut(s) 63
BseRI GAGGAG 3 cut(s) 194, 197, 200
BseXI GCAGC 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 271
BsiEI CGRYCG 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 82
BsiSI CCGG 2 cut(s) 102, 128
BslI CCNNNNNNNGG 2 cut(s) 249, 250
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 2 cut(s) 130, 268
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 1 cut(s) 241
BspMAI CTGCAG 1 cut(s) 338
BspPI GGATC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 63
BsrFI RCCGGY 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 101
BssMI GATC 2 cut(s) 130, 268
Bst6I CTCTTC 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 52
BstKTI GATC 2 cut(s) 133, 271
BstMBI GATC 2 cut(s) 130, 268
BstMCI CGRYCG 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 334
BstV1I GCAGC 1 cut(s) 320
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 343
CciI TCATGA 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 101
Csp6I GTAC 1 cut(s) 300
CspCI CAANNNNNGTGG 2 cut(s) 45, 80
CviAII CATG 2 cut(s) 46, 88
CviJI RGCY 6 cut(s) 119, 144, 212, 234, 242, 333
CviKI_1 RGCY 6 cut(s) 119, 144, 212, 234, 242, 333
CviQI GTAC 1 cut(s) 300
DdeI CTNAG 1 cut(s) 52
DpnI GATC 2 cut(s) 132, 270
DpnII GATC 2 cut(s) 130, 268
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 244
Eco88I CYCGRG 1 cut(s) 82
EcoT22I ATGCAT 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 244
FaeI CATG 2 cut(s) 49, 91
FaiI YATR 8 cut(s) 20, 47, 89, 230, 237, 265, 291, 349
FatI CATG 2 cut(s) 45, 87
Fnu4HI GCNGC 1 cut(s) 334
FriOI GRGCYC 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 334
FspBI CTAG 1 cut(s) 245
GluI GCNGC 1 cut(s) 334
HapII CCGG 2 cut(s) 102, 128
Hin1II CATG 2 cut(s) 49, 91
HincII GTYRAC 1 cut(s) 72
HindII GTYRAC 1 cut(s) 72
HinfI GANTC 1 cut(s) 68
HpaII CCGG 2 cut(s) 102, 128
HphI GGTGA 3 cut(s) 116, 164, 203
Hpy166II GTNNAC 1 cut(s) 72
Hpy188III TCNNGA 2 cut(s) 46, 306
Hpy8I GTNNAC 1 cut(s) 72
Hpy99I CGWCG 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 228, 336, 356
HpyF3I CTNAG 1 cut(s) 52
Hsp92II CATG 2 cut(s) 49, 91
Kzo9I GATC 2 cut(s) 130, 268
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 5 cut(s) 115, 141, 176, 291, 296
Lsp1109I GCAGC 1 cut(s) 320
LweI GCATC 1 cut(s) 215
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 2 cut(s) 95, 191
MalI GATC 2 cut(s) 132, 270
MboI GATC 2 cut(s) 130, 268
MboII GAAGA 2 cut(s) 188, 218
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 3 cut(s) 21, 30, 292
MlyI GAGTC 1 cut(s) 77
Mph1103I ATGCAT 1 cut(s) 230
MseI TTAA 1 cut(s) 171
MspI CCGG 2 cut(s) 102, 128
NdeII GATC 2 cut(s) 130, 268
NlaIII CATG 2 cut(s) 49, 91
NlaIV GGNNCC 1 cut(s) 241
NmuCI GTSAC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 230
PaeR7I CTCGAG 1 cut(s) 82
PagI TCATGA 1 cut(s) 45
PkrI GCNGC 1 cut(s) 335
Ple19I CGATCG 1 cut(s) 271
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
PsiI TTATAA 2 cut(s) 20, 291
PspN4I GGNNCC 1 cut(s) 241
PspXI VCTCGAGB 1 cut(s) 82
PstI CTGCAG 1 cut(s) 338
PvuI CGATCG 1 cut(s) 271
RsaI GTAC 1 cut(s) 301
RsaNI GTAC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 1 cut(s) 334
Sau3AI GATC 2 cut(s) 130, 268
SchI GAGTC 1 cut(s) 77
SduI GDGCHC 1 cut(s) 244
SetI ASST 5 cut(s) 53, 121, 214, 236, 315
SfaNI GCATC 1 cut(s) 215
SfcI CTRYAG 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 82
SgrAI CRCCGGYG 1 cut(s) 101
SlaI CTCGAG 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 3 cut(s) 21, 30, 292
SspMI CTAG 1 cut(s) 245
TaqI TCGA 2 cut(s) 83, 271
TasI AATT 3 cut(s) 21, 30, 292
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 1 cut(s) 343
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 1 cut(s) 333
Tsp45I GTSAC 1 cut(s) 191
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 82
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.