MD17G1237000.v1.1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008229
Physical Location & Seq
Forward (+)
28595976 .. 28596350
375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1237000.v1.1.491

Sequence Viewer

Length: 375 bp
ATGACGAATAATATTACAGTTTCAGTAATTATTCTCCTTGTCATGACCTTGACATTGGGGGTGGAGTCAATCGAATACTCAAGCATGAGGCGTAATGCCGGTGAAGTAGAAGTAACTTCACCACTGGATCCTTGCGATGGCCTGTTGGGTGAGGACTGCCTGAATATTAAAGAAGAGGAGGAGGGCAACGAGGAGGGTTACGAGGACACGGAAGAAGCTAAAACCATAAGATATATAAGTTATGGAGCCCTACAGAGGGATAATGTTCCATGCGATCGACGAGGCAGTTCTTATTATAGTTGTGGTACATCTGGACAGGTCAATCCTTACCATCGTGGCTGCAGTGTGATTACATATTGTGCAAGGAACGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RALF

Protein Analysis

125

Amino Acids

13.69

Weight (kDa)

4.43

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 72 - 122 4.8e-21 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 122, 135
AfaI GTAC 1 cut(s) 307
AfiI CCNNNNNNNGG 3 cut(s) 137, 255, 256
AluBI AGCT 1 cut(s) 218
AluI AGCT 1 cut(s) 218
AlwI GGATC 2 cut(s) 122, 135
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 1 cut(s) 339
AsuHPI GGTGA 3 cut(s) 111, 113, 161
BamHI GGATCC 1 cut(s) 127
BanII GRGCYC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 326
BccI CCATC 2 cut(s) 131, 339
BfmI CTRYAG 2 cut(s) 251, 340
BisI GCNGC 1 cut(s) 340
BlsI GCNGC 1 cut(s) 341
BmiI GGNNCC 2 cut(s) 129, 247
BpuEI CTTGAG 1 cut(s) 64
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 255, 256
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 1 cut(s) 129
BseLI CCNNNNNNNGG 3 cut(s) 137, 255, 256
BseNI ACTGG 1 cut(s) 129
BseRI GAGGAG 3 cut(s) 191, 194, 206
BseXI GCAGC 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 1 cut(s) 141
BsiEI CGRYCG 1 cut(s) 277
BsiSI CCGG 1 cut(s) 99
BslI CCNNNNNNNGG 3 cut(s) 137, 255, 256
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 250
Bsp143I GATC 2 cut(s) 127, 274
BspANI GGCC 1 cut(s) 141
BspHI TCATGA 1 cut(s) 42
BspLI GGNNCC 2 cut(s) 129, 247
BspMAI CTGCAG 1 cut(s) 344
BspPI GGATC 2 cut(s) 122, 135
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 98
BssMI GATC 2 cut(s) 127, 274
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 168
BstKTI GATC 2 cut(s) 130, 277
BstMBI GATC 2 cut(s) 127, 274
BstMCI CGRYCG 1 cut(s) 277
BstSFI CTRYAG 2 cut(s) 251, 340
BstV1I GCAGC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BsuRI GGCC 1 cut(s) 141
BtgZI GCGATG 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 349
BtsIMutI CAGTG 2 cut(s) 122, 349
CciI TCATGA 1 cut(s) 42
Cfr10I RCCGGY 1 cut(s) 98
Csp6I GTAC 1 cut(s) 306
CviAII CATG 3 cut(s) 43, 85, 270
CviJI RGCY 4 cut(s) 141, 218, 248, 339
CviKI_1 RGCY 4 cut(s) 141, 218, 248, 339
CviQI GTAC 1 cut(s) 306
DpnI GATC 2 cut(s) 129, 276
DpnII GATC 2 cut(s) 127, 274
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 250
FaeI CATG 3 cut(s) 46, 88, 273
FaiI YATR 9 cut(s) 44, 86, 227, 234, 236, 243, 271, 297, 355
FatI CATG 3 cut(s) 42, 84, 269
Fnu4HI GCNGC 1 cut(s) 340
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 1 cut(s) 340
GluI GCNGC 1 cut(s) 340
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 99
Hin1II CATG 3 cut(s) 46, 88, 273
HinfI GANTC 1 cut(s) 65
HpaII CCGG 1 cut(s) 99
HphI GGTGA 3 cut(s) 111, 113, 161
Hpy188III TCNNGA 2 cut(s) 43, 312
Hpy99I CGWCG 1 cut(s) 282
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 2 cut(s) 342, 362
Hsp92II CATG 3 cut(s) 46, 88, 273
Kzo9I GATC 2 cut(s) 127, 274
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 6 cut(s) 110, 112, 155, 173, 297, 302
Lsp1109I GCAGC 1 cut(s) 326
MaeIII GTNAC 2 cut(s) 112, 197
MalI GATC 2 cut(s) 129, 276
MboI GATC 2 cut(s) 127, 274
MboII GAAGA 2 cut(s) 185, 224
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 1 cut(s) 250
MluCI AATT 1 cut(s) 27
MlyI GAGTC 1 cut(s) 74
MseI TTAA 1 cut(s) 168
MspI CCGG 1 cut(s) 99
NdeII GATC 2 cut(s) 127, 274
NlaIII CATG 3 cut(s) 46, 88, 273
NlaIV GGNNCC 2 cut(s) 129, 247
PagI TCATGA 1 cut(s) 42
PkrI GCNGC 1 cut(s) 341
Ple19I CGATCG 1 cut(s) 277
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PspN4I GGNNCC 2 cut(s) 129, 247
PstI CTGCAG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 127
PvuI CGATCG 1 cut(s) 277
RsaI GTAC 1 cut(s) 307
RsaNI GTAC 1 cut(s) 306
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 1 cut(s) 340
Sau3AI GATC 2 cut(s) 127, 274
SchI GAGTC 1 cut(s) 74
SduI GDGCHC 1 cut(s) 250
SetI ASST 3 cut(s) 50, 220, 321
SfcI CTRYAG 2 cut(s) 251, 340
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
Sse9I AATT 1 cut(s) 27
SspI AATATT 2 cut(s) 13, 166
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 2 cut(s) 72, 277
TasI AATT 1 cut(s) 27
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 2 cut(s) 129, 349
TseI GCWGC 1 cut(s) 339
TspGWI ACGGA 1 cut(s) 224
TspRI CASTG 2 cut(s) 129, 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.