AT3G32930

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008584
Physical Location & Seq
Forward (+)
13489249 .. 13490906
1658 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G32930.2

Sequence Viewer

Length: 750 bp
ATGCAACTCTCACTGGTTCAAGTCTCTTCAGTGTCCAACTTCAGATCCCAATCAACGATTCCTACTCTCTCCAACTCAAACCCATCTTGTTTACTTCTTCAAAAGTCCATCTTTCCTGGGTCCAAGTTAACCCTCCATCGAATTTTTCGTTATCCGAAGAAAATCTCAAATGGCTCTACTCGTGCTTCACTGCTTGAGACCCCTATCTTATGGGCTGGTCGGATTTGTGTCTTCTACGCTCTCGTGAAAGCTGGCTTTGCTGGATCCAAGTCTAACCCTATCGTTTCTGGTTTGGATACTGGTGGTGTTGATGTTGAATATGATGATGGTGCTGATCTTGGTTTCTCAAAGTGGCTTCAGAACATTAAGGGCAACAAACCAGATAAGGATGCAGCTGATAAGAGGAAGCTAGTGAGCAAATGGCACCCAACGACAAAGGGAACACTTAGAAGGAACTACAGGATACCTTCGAAAGCCGAAGGAAACCGTTTGCTTAAAGCCATTGCGTCTCTTCTCTCAGATGATGATCATTTTAGAGATGCAACATCTCACAAGGGTTGTCAAATACGGAGGGAGAGTGCGCACGGTCAAAGCGTATGTTGCAACAATGTGAGAGCTCTGTTTGATGAGTTACCGACGCCACATTTGGTGGTGGAGATCACACCTTTTCCAGCCGGACCGCTCACAGAGAATGATTACCTTAAGGCTGAGAAGCTGGAGAGGATTCTTAGGTCTGGCGCCAACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.43

Weight (kDa)

9.61

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 582
AccB1I GGYRCC 2 cut(s) 423, 737
AccBSI CCGCTC 1 cut(s) 682
AciI CCGC 1 cut(s) 680
AclWI GGATC 3 cut(s) 39, 258, 271
AcsI RAATTY 1 cut(s) 141
AcuI CTGAAG 3 cut(s) 12, 25, 341
AcyI GRCGYC 2 cut(s) 638, 738
AflII CTTAAG 1 cut(s) 701
AgsI TTSAA 3 cut(s) 20, 101, 317
AjnI CCWGG 1 cut(s) 115
AjuI GAANNNNNNNTTGG 2 cut(s) 42, 74
AluBI AGCT 5 cut(s) 251, 395, 409, 617, 715
AluI AGCT 5 cut(s) 251, 395, 409, 617, 715
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 3 cut(s) 28, 191, 513
AlwI GGATC 3 cut(s) 39, 258, 271
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 1 cut(s) 141
ArsI GACNNNNNNTTYG 2 cut(s) 628, 660
AspLEI GCGC 2 cut(s) 583, 740
AspS9I GGNCC 2 cut(s) 120, 677
AsuII TTCGAA 1 cut(s) 470
AvaII GGWCC 2 cut(s) 120, 677
BamHI GGATCC 1 cut(s) 263
BanI GGYRCC 2 cut(s) 423, 737
BanII GRGCYC 1 cut(s) 619
BauI CACGAG 2 cut(s) 180, 242
BbsI GAAGAC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 1 cut(s) 404
BccI CCATC 4 cut(s) 91, 116, 144, 320
BciT130I CCWGG 1 cut(s) 117
BciVI GTATCC 2 cut(s) 289, 456
BclI TGATCA 1 cut(s) 526
BcoDI GTCTC 3 cut(s) 28, 191, 513
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 1 cut(s) 457
BfoI RGCGCY 1 cut(s) 741
BfrI CTTAAG 1 cut(s) 701
BfuI GTATCC 2 cut(s) 289, 456
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 2 cut(s) 120, 677
BmgT120I GGNCC 2 cut(s) 120, 677
BmiI GGNNCC 4 cut(s) 121, 265, 425, 739
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 2 cut(s) 379, 529
BpiI GAAGAC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 737
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 215
BsaBI GATNNNNATC 2 cut(s) 49, 525
BsaHI GRCGYC 2 cut(s) 638, 738
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 116
BsaXI ACNNNNNCTCC 2 cut(s) 562, 592
Bse1I ACTGG 2 cut(s) 18, 304
Bse3DI GCAATG 1 cut(s) 501
Bse8I GATNNNNATC 2 cut(s) 49, 525
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 1 cut(s) 116
BseGI GGATG 1 cut(s) 394
BseJI GATNNNNATC 2 cut(s) 49, 525
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 2 cut(s) 531, 699
BseNI ACTGG 2 cut(s) 18, 304
BseXI GCAGC 1 cut(s) 404
BshNI GGYRCC 2 cut(s) 423, 737
BsiHKAI GWGCWC 1 cut(s) 619
BsiSI CCGG 1 cut(s) 675
BsmAI GTCTC 3 cut(s) 28, 191, 513
BsmBI CGTCTC 1 cut(s) 513
Bso31I GGTCTC 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 619
Bsp143I GATC 5 cut(s) 44, 263, 334, 526, 657
BspACI CCGC 1 cut(s) 680
BspCNI CTCAG 2 cut(s) 530, 700
BspLI GGNNCC 4 cut(s) 121, 265, 425, 739
BspPI GGATC 3 cut(s) 39, 258, 271
BspT104I TTCGAA 1 cut(s) 470
BspT107I GGYRCC 2 cut(s) 423, 737
BspTI CTTAAG 1 cut(s) 701
BspTNI GGTCTC 1 cut(s) 191
BsrBI CCGCTC 1 cut(s) 682
BsrDI GCAATG 1 cut(s) 501
BsrI ACTGG 2 cut(s) 18, 304
BssECI CCNNGG 1 cut(s) 116
BssMI GATC 5 cut(s) 44, 263, 334, 526, 657
BssNI GRCGYC 2 cut(s) 638, 738
BssSI CACGAG 2 cut(s) 180, 242
Bst2BI CACGAG 2 cut(s) 180, 242
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 2 cut(s) 488, 587
Bst6I CTCTTC 2 cut(s) 31, 516
BstACI GRCGYC 2 cut(s) 638, 738
BstAFI CTTAAG 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 470
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 446, 517, 708, 728
BstF5I GGATG 1 cut(s) 394
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 2 cut(s) 583, 740
BstKTI GATC 5 cut(s) 47, 266, 337, 529, 660
BstMAI GTCTC 3 cut(s) 28, 191, 513
BstMBI GATC 5 cut(s) 44, 263, 334, 526, 657
BstMWI GCNNNNNNNGC 2 cut(s) 257, 600
BstNI CCWGG 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 457
BstV1I GCAGC 1 cut(s) 404
BstV2I GAAGAC 1 cut(s) 223
BstX2I RGATCY 2 cut(s) 44, 263
BstYI RGATCY 2 cut(s) 44, 263
BsuI GTATCC 2 cut(s) 289, 456
BtsCI GGATG 1 cut(s) 394
BtsI GCAGTG 1 cut(s) 188
BtsIMutI CAGTG 3 cut(s) 11, 36, 188
Cac8I GCNNGC 1 cut(s) 253
CfoI GCGC 2 cut(s) 583, 740
Cfr13I GGNCC 2 cut(s) 120, 677
CpoI CGGWCCG 1 cut(s) 677
CseI GACGC 2 cut(s) 495, 646
CspI CGGWCCG 1 cut(s) 677
DdeI CTNAG 4 cut(s) 446, 517, 708, 728
DinI GGCGCC 1 cut(s) 739
DpnI GATC 5 cut(s) 46, 265, 336, 528, 659
DpnII GATC 5 cut(s) 44, 263, 334, 526, 657
Eam1104I CTCTTC 2 cut(s) 31, 516
EarI CTCTTC 2 cut(s) 31, 516
Ecl136II GAGCTC 1 cut(s) 617
Eco24I GRGCYC 1 cut(s) 619
Eco31I GGTCTC 1 cut(s) 191
Eco47I GGWCC 2 cut(s) 120, 677
Eco53kI GAGCTC 1 cut(s) 617
Eco57I CTGAAG 3 cut(s) 12, 25, 341
EcoICRI GAGCTC 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 619
EgeI GGCGCC 1 cut(s) 739
EheI GGCGCC 1 cut(s) 739
Esp3I CGTCTC 1 cut(s) 513
FaiI YATR 3 cut(s) 211, 321, 598
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FbaI TGATCA 1 cut(s) 526
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 1 cut(s) 401
FriOI GRGCYC 1 cut(s) 619
Fsp4HI GCNGC 1 cut(s) 393
FspAI RTGCGCAY 1 cut(s) 582
FspBI CTAG 1 cut(s) 410
FspI TGCGCA 1 cut(s) 582
GlaI GCGC 2 cut(s) 582, 739
GluI GCNGC 1 cut(s) 393
GsuI CTGGAG 1 cut(s) 737
HaeII RGCGCY 1 cut(s) 741
HapII CCGG 1 cut(s) 675
HgaI GACGC 2 cut(s) 495, 646
HhaI GCGC 2 cut(s) 583, 740
Hin1I GRCGYC 2 cut(s) 638, 738
Hin6I GCGC 2 cut(s) 581, 738
HinP1I GCGC 2 cut(s) 581, 738
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HinfI GANTC 2 cut(s) 58, 724
HpaI GTTAAC 1 cut(s) 129
HpaII CCGG 1 cut(s) 675
Hpy166II GTNNAC 2 cut(s) 92, 129
Hpy188I TCNGA 5 cut(s) 44, 156, 222, 360, 520
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 2 cut(s) 92, 129
Hpy99I CGWCG 1 cut(s) 640
HpyAV CCTTC 3 cut(s) 444, 473, 477
HpyCH4III ACNGT 2 cut(s) 488, 587
HpyCH4V TGCA 4 cut(s) 4, 392, 542, 603
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 600
HpyF3I CTNAG 4 cut(s) 446, 517, 708, 728
Hsp92I GRCGYC 2 cut(s) 638, 738
HspAI GCGC 2 cut(s) 581, 738
KasI GGCGCC 1 cut(s) 737
Ksp22I TGATCA 1 cut(s) 526
KspAI GTTAAC 1 cut(s) 129
Kzo9I GATC 5 cut(s) 44, 263, 334, 526, 657
Lsp1109I GCAGC 1 cut(s) 404
LweI GCATC 2 cut(s) 379, 529
MaeI CTAG 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 630
MalI GATC 5 cut(s) 46, 265, 336, 528, 659
MbiI CCGCTC 1 cut(s) 682
MboI GATC 5 cut(s) 44, 263, 334, 526, 657
MboII GAAGA 5 cut(s) 18, 89, 169, 223, 503
MflI RGATCY 2 cut(s) 44, 263
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 1 cut(s) 141
Mly113I GGCGCC 1 cut(s) 738
MmeI TCCRAC 3 cut(s) 60, 96, 200
MnlI CCTC 4 cut(s) 143, 396, 564, 714
MseI TTAA 4 cut(s) 128, 366, 495, 702
MspA1I CMGCKG 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 701
MspI CCGG 1 cut(s) 675
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 257, 600
NarI GGCGCC 1 cut(s) 738
NdeII GATC 5 cut(s) 44, 263, 334, 526, 657
NlaIV GGNNCC 4 cut(s) 121, 265, 425, 739
NsbI TGCGCA 1 cut(s) 582
NspV TTCGAA 1 cut(s) 470
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 591
PfeI GAWTC 2 cut(s) 58, 724
PkrI GCNGC 1 cut(s) 394
PluTI GGCGCC 1 cut(s) 741
Psp124BI GAGCTC 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 4 cut(s) 121, 265, 425, 739
PspPI GGNCC 2 cut(s) 120, 677
PsuI RGATCY 2 cut(s) 44, 263
PvuII CAGCTG 1 cut(s) 395
Rsr2I CGGWCCG 1 cut(s) 677
RsrII CGGWCCG 1 cut(s) 677
SacI GAGCTC 1 cut(s) 619
SaqAI TTAA 4 cut(s) 128, 366, 495, 702
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 5 cut(s) 44, 263, 334, 526, 657
Sau96I GGNCC 2 cut(s) 120, 677
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 619
SetI ASST 9 cut(s) 253, 397, 411, 469, 619, 667, 702, 717, 734
SfaNI GCATC 2 cut(s) 379, 529
SfcI CTRYAG 1 cut(s) 457
SfoI GGCGCC 1 cut(s) 739
SfuI TTCGAA 1 cut(s) 470
SinI GGWCC 2 cut(s) 120, 677
SmlI CTYRAG 2 cut(s) 194, 701
SmoI CTYRAG 2 cut(s) 194, 701
Sse9I AATT 1 cut(s) 141
SsiI CCGC 1 cut(s) 680
SspDI GGCGCC 1 cut(s) 737
SspMI CTAG 1 cut(s) 410
SstI GAGCTC 1 cut(s) 619
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 488, 587
TaqI TCGA 2 cut(s) 139, 470
TasI AATT 1 cut(s) 141
TfiI GAWTC 2 cut(s) 58, 724
Tru1I TTAA 4 cut(s) 128, 366, 495, 702
Tru9I TTAA 4 cut(s) 128, 366, 495, 702
TscAI CASTG 3 cut(s) 18, 36, 195
TseI GCWGC 1 cut(s) 392
TspGWI ACGGA 1 cut(s) 583
TspRI CASTG 3 cut(s) 18, 36, 195
Vha464I CTTAAG 1 cut(s) 701
VpaK11BI GGWCC 2 cut(s) 120, 677
XapI RAATTY 1 cut(s) 141
XspI CTAG 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.