Rorug05G0477100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008584
Physical Location & Seq
Forward (+)
65650962 .. 65653112
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0477100.1

Sequence Viewer

Length: 855 bp
ATGCATGGATTTCTGGCTCTACTGCCTCTAACACCACCACACCCACAGCCACACCTCTATTTCTTCAACCTCTCTTCTTCTCCTTCAAGATCTCTTCTTTACCTGCAAATCAGACCCAAATCCTCGACACCCATTTTGGGATACCCCTCAAAAACCAACCTGAATCTCCTCGTGTTCTCCTCCCACTCTTCGAGTTTCTCGAATTTCAATAGCAAACGACGACGCTTTGTTACGAGAGCGAGTCGCAGAGAGTCCCCGTACCAAGTTCTGGGGGTGTCCCCTTCTGCTACTGATGGGGAGATCAAGAAGGCGTATCGGAAACTTGCGCTCAAGTATCACCCAGATGTTAATAAAGAGCCAAATGCGCAGGAGAAATTTATGAGGATTAAACATGCCTATAATACATTGCTGAATTCAAAATCTCGGGGGAAATATGACTCTAATTACGGATCTGACTATTCCTATTCTGGTTCTCAAAGAAGCCAAAGTAAGAAGGCTCAAGATGAAGAAGAGTTTTATGGATTCGGGGACTTCTTTAAAGATATTCAAGCAGAATTCCAGAACTGGGAAGAAAGTGCTTCTACACAAGGAAAGCCAAGGAGTCTGTGGGAGGAATTGTCGGAAATTGGAGAAGAATTTGTAGAATTTCTCGAGAAAGAACTCAACATAACGGATCCAGAGGTTGGATCAGATAACATCAATGAAGGGCCTGAGAAGGGTCCATTCAGAAATTCTGGGAAAGAGACAACGGAAAATGCAAGTAAAAAGGAAGCCAGTATGGGGGCTAGTATTGAAGAGAATATTGATGAGATAGAAGCTACTCTTGCTCAGTTGAAAAAGGAATTGGGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

32.2

Weight (kDa)

6.35

Isoelectric Point (pI)

63.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 86 - 146 2.4e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 366
Acc36I ACCTGC 1 cut(s) 111
AccB7I CCANNNNNTGG 2 cut(s) 268, 683
AclWI GGATC 4 cut(s) 457, 668, 681, 694
AcsI RAATTY 7 cut(s) 202, 374, 412, 554, 635, 644, 730
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 6 cut(s) 137, 268, 565, 683, 716, 780
AgsI TTSAA 7 cut(s) 67, 87, 208, 417, 548, 794, 835
AjuI GAANNNNNNNTTGG 1 cut(s) 827
AluBI AGCT 1 cut(s) 818
AluI AGCT 1 cut(s) 818
Alw26I GTCTC 1 cut(s) 737
AlwI GGATC 4 cut(s) 457, 668, 681, 694
Ama87I CYCGRG 2 cut(s) 423, 650
AoxI GGCC 1 cut(s) 707
ApoI RAATTY 7 cut(s) 202, 374, 412, 554, 635, 644, 730
ArsI GACNNNNNNTTYG 2 cut(s) 118, 150
AspLEI GCGC 2 cut(s) 328, 367
AspS9I GGNCC 2 cut(s) 707, 719
AsuHPI GGTGA 1 cut(s) 329
AvaI CYCGRG 2 cut(s) 423, 650
AvaII GGWCC 1 cut(s) 719
BamHI GGATCC 1 cut(s) 673
BauI CACGAG 1 cut(s) 170
BccI CCATC 1 cut(s) 287
BciVI GTATCC 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 737
BfaI CTAG 1 cut(s) 786
BfuAI ACCTGC 1 cut(s) 111
BfuI GTATCC 1 cut(s) 134
BglII AGATCT 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 719
BmeT110I CYCGRG 2 cut(s) 423, 650
BmgT120I GGNCC 2 cut(s) 707, 719
BmiI GGNNCC 2 cut(s) 675, 720
BmrI ACTGGG 1 cut(s) 574
BmuI ACTGGG 1 cut(s) 574
BpuEI CTTGAG 2 cut(s) 314, 483
BsaJI CCNNGG 1 cut(s) 596
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 6 cut(s) 137, 268, 565, 683, 716, 780
Bse1I ACTGG 2 cut(s) 569, 774
Bse3DI GCAATG 1 cut(s) 404
BseDI CCNNGG 1 cut(s) 596
BseLI CCNNNNNNNGG 6 cut(s) 137, 268, 565, 683, 716, 780
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 2 cut(s) 702, 842
BseNI ACTGG 2 cut(s) 569, 774
BseRI GAGGAG 2 cut(s) 158, 169
BshFI GGCC 1 cut(s) 709
BsiHKCI CYCGRG 2 cut(s) 423, 650
BslFI GGGAC 3 cut(s) 238, 262, 542
BslI CCNNNNNNNGG 6 cut(s) 137, 268, 565, 683, 716, 780
BsmAI GTCTC 1 cut(s) 737
BsmFI GGGAC 3 cut(s) 238, 262, 542
BsnI GGCC 1 cut(s) 709
BsoBI CYCGRG 2 cut(s) 423, 650
Bsp143I GATC 5 cut(s) 89, 300, 449, 673, 686
BspANI GGCC 1 cut(s) 709
BspCNI CTCAG 2 cut(s) 703, 841
BspLI GGNNCC 2 cut(s) 675, 720
BspMI ACCTGC 1 cut(s) 111
BspPI GGATC 4 cut(s) 457, 668, 681, 694
BsrDI GCAATG 1 cut(s) 404
BsrI ACTGG 2 cut(s) 569, 774
BssECI CCNNGG 1 cut(s) 596
BssMI GATC 5 cut(s) 89, 300, 449, 673, 686
BssSI CACGAG 1 cut(s) 170
BssT1I CCWWGG 1 cut(s) 596
Bst2BI CACGAG 1 cut(s) 170
Bst6I CTCTTC 5 cut(s) 79, 99, 193, 504, 789
BstDEI CTNAG 2 cut(s) 711, 828
BstHHI GCGC 2 cut(s) 328, 367
BstKTI GATC 5 cut(s) 92, 303, 452, 676, 689
BstMAI GTCTC 1 cut(s) 737
BstMBI GATC 5 cut(s) 89, 300, 449, 673, 686
BstMWI GCNNNNNNNGC 2 cut(s) 364, 824
BstNSI RCATGY 1 cut(s) 395
BstX2I RGATCY 3 cut(s) 89, 449, 673
BstYI RGATCY 3 cut(s) 89, 449, 673
BsuI GTATCC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 709
BveI ACCTGC 1 cut(s) 111
CfoI GCGC 2 cut(s) 328, 367
Cfr13I GGNCC 2 cut(s) 707, 719
CseI GACGC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 259
CviAII CATG 2 cut(s) 5, 392
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 2 cut(s) 711, 828
DpnI GATC 5 cut(s) 91, 302, 451, 675, 688
DpnII GATC 5 cut(s) 89, 300, 449, 673, 686
DraI TTTAAA 1 cut(s) 538
Eam1104I CTCTTC 5 cut(s) 79, 99, 193, 504, 789
EarI CTCTTC 5 cut(s) 79, 99, 193, 504, 789
Eco130I CCWWGG 1 cut(s) 596
Eco47I GGWCC 1 cut(s) 719
Eco88I CYCGRG 2 cut(s) 423, 650
EcoO109I RGGNCCY 1 cut(s) 707
EcoRI GAATTC 2 cut(s) 412, 554
EcoT14I CCWWGG 1 cut(s) 596
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 596
FaeI CATG 2 cut(s) 8, 395
FaiI YATR 8 cut(s) 6, 380, 393, 399, 435, 519, 668, 779
FalI AAGNNNNNCTT 2 cut(s) 807, 839
FaqI GGGAC 3 cut(s) 238, 262, 542
FatI CATG 2 cut(s) 4, 391
FspBI CTAG 1 cut(s) 786
FspI TGCGCA 1 cut(s) 366
GlaI GCGC 2 cut(s) 327, 366
HaeIII GGCC 1 cut(s) 709
HgaI GACGC 1 cut(s) 231
HhaI GCGC 2 cut(s) 328, 367
Hin1II CATG 2 cut(s) 8, 395
Hin6I GCGC 2 cut(s) 326, 365
HinP1I GCGC 2 cut(s) 326, 365
HinfI GANTC 6 cut(s) 163, 241, 251, 437, 522, 601
HphI GGTGA 1 cut(s) 329
Hpy188I TCNGA 6 cut(s) 113, 318, 454, 622, 691, 728
Hpy188III TCNNGA 8 cut(s) 87, 199, 304, 500, 559, 650, 652, 677
Hpy99I CGWCG 2 cut(s) 222, 225
HpyAV CCTTC 6 cut(s) 93, 291, 301, 487, 698, 709
HpyCH4V TGCA 3 cut(s) 4, 106, 758
HpyF10VI GCNNNNNNNGC 2 cut(s) 364, 824
HpyF3I CTNAG 2 cut(s) 711, 828
Hsp92II CATG 2 cut(s) 8, 395
HspAI GCGC 2 cut(s) 326, 365
Kzo9I GATC 5 cut(s) 89, 300, 449, 673, 686
MaeI CTAG 1 cut(s) 786
MaeIII GTNAC 1 cut(s) 229
MalI GATC 5 cut(s) 91, 302, 451, 675, 688
MboI GATC 5 cut(s) 89, 300, 449, 673, 686
MflI RGATCY 3 cut(s) 89, 449, 673
MlyI GAGTC 4 cut(s) 250, 260, 431, 610
MmeI TCCRAC 2 cut(s) 600, 664
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 348, 387, 537
MslI CAYNNNNRTG 1 cut(s) 342
MwoI GCNNNNNNNGC 2 cut(s) 364, 824
NdeII GATC 5 cut(s) 89, 300, 449, 673, 686
NlaIII CATG 2 cut(s) 8, 395
NlaIV GGNNCC 2 cut(s) 675, 720
NsbI TGCGCA 1 cut(s) 366
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 395
PaeR7I CTCGAG 1 cut(s) 650
PcsI WCGNNNNNNNCGW 1 cut(s) 197
PfeI GAWTC 2 cut(s) 163, 522
PflMI CCANNNNNTGG 2 cut(s) 268, 683
PleI GAGTC 4 cut(s) 249, 259, 431, 609
PpsI GAGTC 4 cut(s) 249, 259, 431, 609
PspN4I GGNNCC 2 cut(s) 675, 720
PspPI GGNCC 2 cut(s) 707, 719
PsuI RGATCY 3 cut(s) 89, 449, 673
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 3 cut(s) 348, 387, 537
Sau3AI GATC 5 cut(s) 89, 300, 449, 673, 686
Sau96I GGNCC 2 cut(s) 707, 719
SchI GAGTC 4 cut(s) 250, 260, 431, 610
SetI ASST 6 cut(s) 57, 72, 105, 162, 684, 820
Sfr274I CTCGAG 1 cut(s) 650
SinI GGWCC 1 cut(s) 719
SlaI CTCGAG 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 3 cut(s) 329, 498, 650
SmoI CTYRAG 3 cut(s) 329, 498, 650
SspI AATATT 1 cut(s) 802
SspMI CTAG 1 cut(s) 786
StyI CCWWGG 1 cut(s) 596
TaqI TCGA 4 cut(s) 125, 191, 200, 651
TfiI GAWTC 2 cut(s) 163, 522
Tru1I TTAA 3 cut(s) 348, 387, 537
Tru9I TTAA 3 cut(s) 348, 387, 537
TspDTI ATGAA 2 cut(s) 519, 717
TspGWI ACGGA 3 cut(s) 462, 686, 764
Van91I CCANNNNNTGG 2 cut(s) 268, 683
VpaK11BI GGWCC 1 cut(s) 719
XapI RAATTY 7 cut(s) 202, 374, 412, 554, 635, 644, 730
XceI RCATGY 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 603
XhoI CTCGAG 1 cut(s) 650
XspI CTAG 1 cut(s) 786
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.