AT3G49630

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005695
Physical Location & Seq
Forward (+)
18397725 .. 18400640
2916 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G49630.2

Sequence Viewer

Length: 999 bp
ATGGCGACAAACTTCAAATCCCTATTACCAGTTATCGACATCAGTCCTTTAGTGGTCAAATGTGATGATGCCAACATGGCGGAGGATGCCGGCGTGGCGGAGGTTGTGGGAAAGCTGGACAGGGCCTGTCGGGACGCAGGATTCTTCTACGTAATTGGTCATGGAATATCGGAGGATTTTATAAGGAAGGTGAGAGTGATGTCGCATCAATTCTTCGAGCTTCCTTATGAAGAGAAACTTAAGATCAAGATTACTCCAGCTGCTGGATACAGAGGATATCAGAGAATCGGACTAAACTTAACGAATGGGAAACAAGATATGCACGAAGCCATTGATTGTTACAAAGAGTTTAAGCAAGGGAAGCATGGGGACATTGGAAAGGTTATGGAAGGGGCAAACCAGTGGCCAGAGAATCCTCAAGAATACAAAGAGTTGATGGAGGAGTATATTAAGCTCTGCATAGATCTTTCTAGAAATATCTTAAGGGGAATCTCATTAGCCCTTGGTGGATCACCTTATGAGTTTGAGGGAAAGATGCTTACAGATCCGTTTTGGATAATGCGTATTCTTGGTTATCCAGGTGTAAACCAAGAAAATGTTATCGGATGTGGAGCTCACACTGACTATGGCTTGTTGTCACTTATAAATCAAGATGATGACAAAACTGCTCTTCAGGTGAGAGACTTGGCCGGCGATTGGATACCAGTTATTCCGATCCCTGGATCATTTGTCTGCAACATCGGTGACATGCTAAAGATTCTTTCTAACGGAGTTTACGAATCCACACTTCATAGAGTGATCAATAACTCTCCCCGATACCGTGTTTGCGTCGGATTCTTTTACGAGACGAACTTCGACGCAGCGGTGGAGCCGTTGGATATCTTCAAAGAGAAGTACCCAGGAAAAGGAACATCCCAAGTTTTCAAAAGAGTTGTCTATGGGGAGCATCTGGTTCACAAACTCCAGACCACTTTTCAAAATTTAGTGGAACACAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

332

Amino Acids

37.46

Weight (kDa)

5.65

Isoelectric Point (pI)

26.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 117 - 209 3.1e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 182, 644
AccB7I CCANNNNNTGG 1 cut(s) 263
AciI CCGC 3 cut(s) 80, 98, 863
AclWI GGATC 4 cut(s) 517, 539, 709, 730
AcoI YGGCCR 2 cut(s) 404, 687
AcsI RAATTY 1 cut(s) 979
AcuI CTGAAG 1 cut(s) 656
AfaI GTAC 1 cut(s) 896
AfiI CCNNNNNNNGG 4 cut(s) 263, 696, 719, 905
AflII CTTAAG 2 cut(s) 239, 481
AgsI TTSAA 4 cut(s) 16, 886, 925, 977
AjnI CCWGG 3 cut(s) 577, 718, 898
AluBI AGCT 5 cut(s) 115, 220, 260, 454, 614
AluI AGCT 5 cut(s) 115, 220, 260, 454, 614
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 2 cut(s) 675, 839
AlwI GGATC 4 cut(s) 517, 539, 709, 730
AlwNI CAGNNNCTG 2 cut(s) 126, 263
AoxI GGCC 3 cut(s) 123, 404, 687
ApeKI GCWGC 2 cut(s) 260, 860
ApoI RAATTY 1 cut(s) 979
ArsI GACNNNNNNTTYG 2 cut(s) 918, 950
AspS9I GGNCC 1 cut(s) 123
AsuHPI GGTGA 4 cut(s) 202, 504, 688, 755
BalI TGGCCA 1 cut(s) 406
BanII GRGCYC 1 cut(s) 616
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 2 cut(s) 247, 872
BccI CCATC 1 cut(s) 430
BceAI ACGGC 1 cut(s) 856
BciT130I CCWGG 3 cut(s) 579, 720, 900
BciVI GTATCC 2 cut(s) 260, 693
BclI TGATCA 1 cut(s) 798
BcoDI GTCTC 2 cut(s) 675, 839
BfaI CTAG 1 cut(s) 471
BfrI CTTAAG 2 cut(s) 239, 481
BfuI GTATCC 2 cut(s) 260, 693
BglI GCCNNNNNGGC 2 cut(s) 77, 95
BglII AGATCT 1 cut(s) 463
BisI GCNGC 2 cut(s) 261, 861
BlsI GCNGC 2 cut(s) 262, 862
Bme1390I CCNGG 3 cut(s) 579, 720, 900
BmgT120I GGNCC 1 cut(s) 123
BmiI GGNNCC 1 cut(s) 870
BmrFI CCNGG 3 cut(s) 579, 720, 900
BmsI GCATC 5 cut(s) 58, 76, 214, 525, 955
BoxI GACNNNNGTC 1 cut(s) 42
BpmI CTGGAG 2 cut(s) 240, 947
BpuEI CTTGAG 1 cut(s) 402
BsaAI YACGTR 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 502, 718, 898
Bsc4I CCNNNNNNNGG 4 cut(s) 263, 696, 719, 905
Bse118I RCCGGY 2 cut(s) 89, 689
Bse1I ACTGG 3 cut(s) 29, 400, 704
BseBI CCWGG 3 cut(s) 579, 720, 900
BseDI CCNNGG 3 cut(s) 502, 718, 898
BseGI GGATG 3 cut(s) 91, 611, 911
BseLI CCNNNNNNNGG 4 cut(s) 263, 696, 719, 905
BseNI ACTGG 3 cut(s) 29, 400, 704
BseRI GAGGAG 1 cut(s) 455
BseXI GCAGC 2 cut(s) 247, 872
BshFI GGCC 3 cut(s) 125, 406, 689
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 2 cut(s) 90, 690
BslFI GGGAC 2 cut(s) 146, 383
BslI CCNNNNNNNGG 4 cut(s) 263, 696, 719, 905
BsmAI GTCTC 2 cut(s) 675, 839
BsmBI CGTCTC 1 cut(s) 839
BsmFI GGGAC 2 cut(s) 146, 383
BsnI GGCC 3 cut(s) 125, 406, 689
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
BspACI CCGC 3 cut(s) 80, 98, 863
BspANI GGCC 3 cut(s) 125, 406, 689
BspLI GGNNCC 1 cut(s) 870
BspPI GGATC 4 cut(s) 517, 539, 709, 730
BspQI GCTCTTC 1 cut(s) 675
BspTI CTTAAG 2 cut(s) 239, 481
BsrFI RCCGGY 2 cut(s) 89, 689
BsrI ACTGG 3 cut(s) 29, 400, 704
BssAI RCCGGY 2 cut(s) 89, 689
BssECI CCNNGG 3 cut(s) 502, 718, 898
BssMI GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
BssT1I CCWWGG 1 cut(s) 502
Bst2UI CCWGG 3 cut(s) 579, 720, 900
Bst4CI ACNGT 2 cut(s) 821, 995
Bst6I CTCTTC 2 cut(s) 225, 675
BstAFI CTTAAG 2 cut(s) 239, 481
BstBAI YACGTR 1 cut(s) 151
BstC8I GCNNGC 2 cut(s) 91, 691
BstF5I GGATG 3 cut(s) 91, 611, 911
BstKTI GATC 7 cut(s) 246, 466, 512, 547, 717, 725, 801
BstMAI GTCTC 2 cut(s) 675, 839
BstMBI GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
BstMWI GCNNNNNNNGC 4 cut(s) 77, 86, 95, 361
BstNI CCWGG 3 cut(s) 579, 720, 900
BstNSI RCATGY 1 cut(s) 751
BstPAI GACNNNNGTC 1 cut(s) 42
BstSCI CCNGG 3 cut(s) 577, 718, 898
BstSNI TACGTA 1 cut(s) 151
BstV1I GCAGC 2 cut(s) 247, 872
BstX2I RGATCY 2 cut(s) 463, 544
BstYI RGATCY 2 cut(s) 463, 544
BsuI GTATCC 2 cut(s) 260, 693
BsuRI GGCC 3 cut(s) 125, 406, 689
BtsCI GGATG 3 cut(s) 91, 611, 911
BtsIMutI CAGTG 2 cut(s) 407, 618
Cac8I GCNNGC 2 cut(s) 91, 691
CaiI CAGNNNCTG 2 cut(s) 126, 263
Cfr10I RCCGGY 2 cut(s) 89, 689
Cfr13I GGNCC 1 cut(s) 123
CseI GACGC 3 cut(s) 143, 817, 866
Csp6I GTAC 1 cut(s) 895
CviAII CATG 4 cut(s) 76, 161, 365, 748
CviQI GTAC 1 cut(s) 895
DpnI GATC 7 cut(s) 245, 465, 511, 546, 716, 724, 800
DpnII GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
EaeI YGGCCR 2 cut(s) 404, 687
Eam1104I CTCTTC 2 cut(s) 225, 675
EarI CTCTTC 2 cut(s) 225, 675
EciI GGCGGA 2 cut(s) 95, 113
Ecl136II GAGCTC 1 cut(s) 614
Eco105I TACGTA 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 616
Eco32I GATATC 2 cut(s) 278, 880
Eco53kI GAGCTC 1 cut(s) 614
Eco57I CTGAAG 1 cut(s) 656
EcoICRI GAGCTC 1 cut(s) 614
EcoO109I RGGNCCY 1 cut(s) 123
EcoRII CCWGG 3 cut(s) 577, 718, 898
EcoRV GATATC 2 cut(s) 278, 880
EcoT14I CCWWGG 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 616
ErhI CCWWGG 1 cut(s) 502
Esp3I CGTCTC 1 cut(s) 839
FaeI CATG 4 cut(s) 79, 164, 368, 751
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FaqI GGGAC 2 cut(s) 146, 383
FatI CATG 4 cut(s) 75, 160, 364, 747
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 2 cut(s) 261, 861
FokI GGATG 3 cut(s) 98, 618, 898
FriOI GRGCYC 1 cut(s) 616
Fsp4HI GCNGC 2 cut(s) 261, 861
FspBI CTAG 1 cut(s) 471
GluI GCNGC 2 cut(s) 261, 861
GsuI CTGGAG 2 cut(s) 240, 947
HaeIII GGCC 3 cut(s) 125, 406, 689
HapII CCGG 2 cut(s) 90, 690
HgaI GACGC 3 cut(s) 143, 817, 866
Hin1II CATG 4 cut(s) 79, 164, 368, 751
HinfI GANTC 7 cut(s) 141, 285, 412, 489, 757, 779, 834
HpaII CCGG 2 cut(s) 90, 690
HphI GGTGA 4 cut(s) 202, 504, 688, 755
Hpy166II GTNNAC 3 cut(s) 586, 775, 955
Hpy188I TCNGA 6 cut(s) 172, 282, 290, 605, 714, 833
Hpy188III TCNNGA 6 cut(s) 131, 247, 419, 471, 650, 964
Hpy8I GTNNAC 3 cut(s) 586, 775, 955
Hpy99I CGWCG 2 cut(s) 833, 860
HpyAV CCTTC 2 cut(s) 181, 383
HpyCH4III ACNGT 2 cut(s) 821, 995
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 3 cut(s) 322, 459, 735
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 86, 95, 361
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 4 cut(s) 79, 164, 368, 751
KroI GCCGGC 2 cut(s) 89, 689
KroNI GCCGGC 2 cut(s) 91, 691
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
LguI GCTCTTC 1 cut(s) 675
LmnI GCTCC 3 cut(s) 611, 868, 943
Lsp1109I GCAGC 2 cut(s) 247, 872
LweI GCATC 5 cut(s) 58, 76, 214, 525, 955
MaeI CTAG 1 cut(s) 471
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 3 cut(s) 338, 636, 743
MalI GATC 7 cut(s) 245, 465, 511, 546, 716, 724, 800
MboI GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
MboII GAAGA 5 cut(s) 136, 205, 242, 662, 874
MflI RGATCY 2 cut(s) 463, 544
MhlI GDGCHC 1 cut(s) 616
MlsI TGGCCA 1 cut(s) 406
MluCI AATT 3 cut(s) 153, 209, 979
MluNI TGGCCA 1 cut(s) 406
MmeI TCCRAC 2 cut(s) 811, 855
MnlI CCTC 7 cut(s) 76, 94, 166, 266, 426, 433, 520
Mox20I TGGCCA 1 cut(s) 406
MroNI GCCGGC 2 cut(s) 89, 689
MscI TGGCCA 1 cut(s) 406
MseI TTAA 6 cut(s) 240, 299, 351, 450, 482, 997
Msp20I TGGCCA 1 cut(s) 406
MspA1I CMGCKG 2 cut(s) 260, 863
MspCI CTTAAG 2 cut(s) 239, 481
MspI CCGG 2 cut(s) 90, 690
MspR9I CCNGG 3 cut(s) 579, 720, 900
MvaI CCWGG 3 cut(s) 579, 720, 900
MwoI GCNNNNNNNGC 4 cut(s) 77, 86, 95, 361
NaeI GCCGGC 2 cut(s) 91, 691
NdeII GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
NgoMIV GCCGGC 2 cut(s) 89, 689
NlaIII CATG 4 cut(s) 79, 164, 368, 751
NlaIV GGNNCC 1 cut(s) 870
NmuCI GTSAC 2 cut(s) 636, 743
NspI RCATGY 1 cut(s) 751
PciSI GCTCTTC 1 cut(s) 675
PcsI WCGNNNNNNNCGW 1 cut(s) 774
PdiI GCCGGC 2 cut(s) 91, 691
PfeI GAWTC 7 cut(s) 141, 285, 412, 489, 757, 779, 834
PflMI CCANNNNNTGG 1 cut(s) 263
PkrI GCNGC 2 cut(s) 262, 862
Ppu21I YACGTR 1 cut(s) 151
PshAI GACNNNNGTC 1 cut(s) 42
PsiI TTATAA 2 cut(s) 182, 644
Psp124BI GAGCTC 1 cut(s) 616
Psp6I CCWGG 3 cut(s) 577, 718, 898
PspGI CCWGG 3 cut(s) 577, 718, 898
PspN4I GGNNCC 1 cut(s) 870
PspPI GGNCC 1 cut(s) 123
PstNI CAGNNNCTG 2 cut(s) 126, 263
PsuI RGATCY 2 cut(s) 463, 544
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
SacI GAGCTC 1 cut(s) 616
SapI GCTCTTC 1 cut(s) 675
SaqAI TTAA 6 cut(s) 240, 299, 351, 450, 482, 997
SatI GCNGC 2 cut(s) 261, 861
Sau3AI GATC 7 cut(s) 243, 463, 509, 544, 714, 722, 798
Sau96I GGNCC 1 cut(s) 123
ScrFI CCNGG 3 cut(s) 579, 720, 900
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 5 cut(s) 58, 76, 214, 525, 955
SmlI CTYRAG 3 cut(s) 239, 417, 481
SmoI CTYRAG 3 cut(s) 239, 417, 481
SnaBI TACGTA 1 cut(s) 151
Sse9I AATT 3 cut(s) 153, 209, 979
SsiI CCGC 3 cut(s) 80, 98, 863
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 616
StyD4I CCNGG 3 cut(s) 577, 718, 898
StyI CCWWGG 1 cut(s) 502
TaaI ACNGT 2 cut(s) 821, 995
TaiI ACGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 36, 216, 855
TasI AATT 3 cut(s) 153, 209, 979
TfiI GAWTC 7 cut(s) 141, 285, 412, 489, 757, 779, 834
Tru1I TTAA 6 cut(s) 240, 299, 351, 450, 482, 997
Tru9I TTAA 6 cut(s) 240, 299, 351, 450, 482, 997
TscAI CASTG 2 cut(s) 407, 625
TseFI GTSAC 2 cut(s) 636, 743
TseI GCWGC 2 cut(s) 260, 860
Tsp45I GTSAC 2 cut(s) 636, 743
TspDTI ATGAA 2 cut(s) 243, 779
TspGWI ACGGA 2 cut(s) 537, 783
TspRI CASTG 2 cut(s) 407, 625
Van91I CCANNNNNTGG 1 cut(s) 263
Vha464I CTTAAG 2 cut(s) 239, 481
XapI RAATTY 1 cut(s) 979
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 1 cut(s) 751
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.