MD02G1177200.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005695
Physical Location & Seq
Forward (+)
15637845 .. 15640944
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1177200.v1.1.491

Sequence Viewer

Length: 990 bp
ATGGCCACCGACTTCAAGTCAATACCCATCATCGATATTGCTCCTCTTTTGGCCAAATCCGATGATCCAAAAATGGGCCAGGACCCGGATGTCGCTCAAGTTGTCAGACAATTGGATCGAGCTTGTAAAGAAGCTGGCTTTTTCTATGTGAAAGGACATGGTATTCCCAAGACTCTGCTGAAACAAGTCAAGAATCTGACACGCAAGTTTTTTGAGCTTCCATACGAAGAAAAACTCAACATCACAATGTCTGCTGATAGCGGATTCAGAGGATATCAGAGAATTGGAGATAATATTACCAAAGGTGTACCTGACATGCATGAAGCTATTGATTGCTATAAAGAATTGAAACCAGGGATGTATGGAGATCTTGGAAAACCAATGGAAGGACCTAACCAATGGCCAGATAATCCCCCAGAATTTAAGGTACTAATGGAGGAATATATCAGCCTTTGCACATGCCTTTCAAAAAAGATCATGCGTGGAATTGCTTTAGCACTGGGTGGATCACCATATGAATTTGAAGGTGATAGAGCTGGAGATGCATTTTGGGTGATGCGTCTTATTGGTTACCCAGCTGCACGCTCTCCAAATGCCTCGGACATTCCAAAAAATGACGTCGGATGTGGAGCTCACACTGATTATGGTTTGTTGACATTGGTTAATCAGGATGACGATATAACTGCACTTCAGGTGAGAAACCATTCCGGTGAGTGGATATCAGCTCCTCCTGTTCCTGGGACATTTGTCTGCAATATCGGAGACATGCTAAAGATTTACTCGAATGGTTTATACGATTCCACTTTGCATCAAGTTATCAACACCGCTCCAAAATACCGGGTCTGTGTAGCGTATTTCTATGAGACAAATTTTGATACAGCAGTGGAGCCTTTGGATTTTTGTGTGGATAAGACAGGTGGATCTAAGAAATTCGAAAGAGCTGTTTATGGAGAACATTTGGTTAACAAGGTCCAAACAAACTTCGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

330

Amino Acids

36.57

Weight (kDa)

5.47

Isoelectric Point (pI)

25.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 8 - 137 8e-33 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 203 - 288 2.3e-20 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AatII GACGTC 1 cut(s) 621
AccBSI CCGCTC 1 cut(s) 827
AciI CCGC 2 cut(s) 261, 825
AclWI GGATC 4 cut(s) 59, 123, 514, 928
AcoI YGGCCR 3 cut(s) 3, 51, 401
AcsI RAATTY 4 cut(s) 419, 518, 868, 929
AcuI CTGAAG 1 cut(s) 674
AcyI GRCGYC 1 cut(s) 618
AdeI CACNNNGTG 1 cut(s) 503
AfaI GTAC 2 cut(s) 309, 429
AfiI CCNNNNNNNGG 5 cut(s) 74, 85, 386, 714, 737
AgsI TTSAA 4 cut(s) 16, 349, 468, 524
AhdI GACNNNNNGTC 1 cut(s) 16
AjnI CCWGG 3 cut(s) 78, 352, 736
AluBI AGCT 9 cut(s) 122, 134, 217, 326, 536, 578, 632, 725, 941
AluI AGCT 9 cut(s) 122, 134, 217, 326, 536, 578, 632, 725, 941
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 2 cut(s) 756, 857
AlwI GGATC 4 cut(s) 59, 123, 514, 928
AoxI GGCC 4 cut(s) 3, 51, 76, 401
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 4 cut(s) 419, 518, 868, 929
Asp700I GAANNNNTTC 1 cut(s) 703
AspS9I GGNCC 4 cut(s) 76, 82, 389, 970
AsuC2I CCSGG 2 cut(s) 86, 839
AsuHPI GGTGA 5 cut(s) 501, 539, 565, 706, 722
AsuII TTCGAA 1 cut(s) 933
AvaII GGWCC 3 cut(s) 82, 389, 970
BaeI ACNNNNGTAYC 2 cut(s) 867, 900
BalI TGGCCA 3 cut(s) 5, 53, 403
BanII GRGCYC 1 cut(s) 634
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 1 cut(s) 565
BccI CCATC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 665, 699
BciT130I CCWGG 3 cut(s) 80, 354, 738
BcnI CCSGG 2 cut(s) 86, 839
BcoDI GTCTC 2 cut(s) 756, 857
BglII AGATCT 1 cut(s) 367
BisI GCNGC 1 cut(s) 579
BlsI GCNGC 1 cut(s) 580
Bme1390I CCNGG 5 cut(s) 80, 86, 354, 738, 839
Bme18I GGWCC 3 cut(s) 82, 389, 970
BmeRI GACNNNNNGTC 1 cut(s) 16
BmgT120I GGNCC 4 cut(s) 76, 82, 389, 970
BmiI GGNNCC 2 cut(s) 84, 888
BmrFI CCNGG 5 cut(s) 80, 86, 354, 738, 839
BmrI ACTGGG 1 cut(s) 509
BmsI GCATC 3 cut(s) 532, 546, 817
BmuI ACTGGG 1 cut(s) 509
BoxI GACNNNNGTC 1 cut(s) 746
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 81
BpuMI CCSGG 2 cut(s) 86, 839
Bsa29I ATCGAT 1 cut(s) 33
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 3 cut(s) 353, 597, 737
BsaWI WCCGGW 1 cut(s) 707
Bsc4I CCNNNNNNNGG 5 cut(s) 74, 85, 386, 714, 737
Bse1I ACTGG 1 cut(s) 504
BseBI CCWGG 3 cut(s) 80, 354, 738
BseCI ATCGAT 1 cut(s) 33
BseDI CCNNGG 3 cut(s) 353, 597, 737
BseGI GGATG 4 cut(s) 94, 363, 629, 676
BseLI CCNNNNNNNGG 5 cut(s) 74, 85, 386, 714, 737
BseNI ACTGG 1 cut(s) 504
BseRI GAGGAG 2 cut(s) 33, 717
BseXI GCAGC 1 cut(s) 565
BseYI CCCAGC 1 cut(s) 574
BsgI GTGCAG 2 cut(s) 564, 669
BshFI GGCC 4 cut(s) 5, 53, 78, 403
BshVI ATCGAT 1 cut(s) 33
BsiHKAI GWGCWC 1 cut(s) 634
BsiSI CCGG 3 cut(s) 86, 708, 838
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 5 cut(s) 74, 85, 386, 714, 737
BsmAI GTCTC 2 cut(s) 756, 857
BsmFI GGGAC 1 cut(s) 754
BsnI GGCC 4 cut(s) 5, 53, 78, 403
Bsp119I TTCGAA 1 cut(s) 933
Bsp1286I GDGCHC 1 cut(s) 634
Bsp143I GATC 6 cut(s) 64, 115, 367, 474, 506, 920
BspACI CCGC 2 cut(s) 261, 825
BspANI GGCC 4 cut(s) 5, 53, 78, 403
BspDI ATCGAT 1 cut(s) 33
BspLI GGNNCC 2 cut(s) 84, 888
BspPI GGATC 4 cut(s) 59, 123, 514, 928
BspT104I TTCGAA 1 cut(s) 933
BsrBI CCGCTC 1 cut(s) 827
BsrI ACTGG 1 cut(s) 504
BssECI CCNNGG 3 cut(s) 353, 597, 737
BssMI GATC 6 cut(s) 64, 115, 367, 474, 506, 920
BssNI GRCGYC 1 cut(s) 618
Bst2UI CCWGG 3 cut(s) 80, 354, 738
BstACI GRCGYC 1 cut(s) 618
BstBI TTCGAA 1 cut(s) 933
BstC8I GCNNGC 2 cut(s) 136, 583
BstDEI CTNAG 1 cut(s) 924
BstEII GGTNACC 1 cut(s) 569
BstF5I GGATG 4 cut(s) 94, 363, 629, 676
BstKTI GATC 6 cut(s) 67, 118, 370, 477, 509, 923
BstMAI GTCTC 2 cut(s) 756, 857
BstMBI GATC 6 cut(s) 64, 115, 367, 474, 506, 920
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 3 cut(s) 80, 354, 738
BstNSI RCATGY 3 cut(s) 319, 462, 769
BstPAI GACNNNNGTC 1 cut(s) 746
BstPI GGTNACC 1 cut(s) 569
BstSCI CCNGG 5 cut(s) 78, 84, 352, 736, 837
BstV1I GCAGC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 367, 920
BstYI RGATCY 2 cut(s) 367, 920
Bsu15I ATCGAT 1 cut(s) 33
BsuRI GGCC 4 cut(s) 5, 53, 78, 403
BsuTUI ATCGAT 1 cut(s) 33
BtsCI GGATG 4 cut(s) 94, 363, 629, 676
BtsI GCAGTG 1 cut(s) 888
BtsIMutI CAGTG 3 cut(s) 497, 636, 888
Cac8I GCNNGC 2 cut(s) 136, 583
Cfr13I GGNCC 4 cut(s) 76, 82, 389, 970
ClaI ATCGAT 1 cut(s) 33
CseI GACGC 1 cut(s) 548
Csp6I GTAC 2 cut(s) 308, 428
CviAII CATG 6 cut(s) 158, 316, 320, 459, 478, 766
CviQI GTAC 2 cut(s) 308, 428
DdeI CTNAG 1 cut(s) 924
DpnI GATC 6 cut(s) 66, 117, 369, 476, 508, 922
DpnII GATC 6 cut(s) 64, 115, 367, 474, 506, 920
DraIII CACNNNGTG 1 cut(s) 503
DrdI GACNNNNNNGTC 1 cut(s) 89
DriI GACNNNNNGTC 1 cut(s) 16
DseDI GACNNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 3 cut(s) 3, 51, 401
Eam1105I GACNNNNNGTC 1 cut(s) 16
Ecl136II GAGCTC 1 cut(s) 632
Eco24I GRGCYC 1 cut(s) 634
Eco32I GATATC 2 cut(s) 275, 720
Eco47I GGWCC 3 cut(s) 82, 389, 970
Eco53kI GAGCTC 1 cut(s) 632
Eco57I CTGAAG 1 cut(s) 674
Eco91I GGTNACC 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 632
EcoO109I RGGNCCY 2 cut(s) 82, 389
EcoO65I GGTNACC 1 cut(s) 569
EcoRII CCWGG 3 cut(s) 78, 352, 736
EcoRV GATATC 2 cut(s) 275, 720
EcoT22I ATGCAT 2 cut(s) 321, 547
EcoT38I GRGCYC 1 cut(s) 634
FaeI CATG 6 cut(s) 161, 319, 323, 462, 481, 769
FaqI GGGAC 1 cut(s) 754
FatI CATG 6 cut(s) 157, 315, 319, 458, 477, 765
FauNDI CATATG 1 cut(s) 514
Fnu4HI GCNGC 1 cut(s) 579
FokI GGATG 4 cut(s) 101, 370, 636, 683
FriOI GRGCYC 1 cut(s) 634
Fsp4HI GCNGC 1 cut(s) 579
GluI GCNGC 1 cut(s) 579
GsaI CCCAGC 1 cut(s) 578
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 4 cut(s) 5, 53, 78, 403
HapII CCGG 3 cut(s) 86, 708, 838
HgaI GACGC 1 cut(s) 548
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 6 cut(s) 161, 319, 323, 462, 481, 769
HincII GTYRAC 2 cut(s) 654, 964
HindII GTYRAC 2 cut(s) 654, 964
HinfI GANTC 4 cut(s) 172, 193, 264, 797
HpaI GTTAAC 1 cut(s) 964
HpaII CCGG 3 cut(s) 86, 708, 838
HphI GGTGA 5 cut(s) 501, 539, 565, 706, 722
Hpy166II GTNNAC 3 cut(s) 308, 654, 964
Hpy188I TCNGA 8 cut(s) 61, 107, 198, 269, 279, 601, 623, 761
Hpy188III TCNNGA 2 cut(s) 190, 668
Hpy8I GTNNAC 3 cut(s) 308, 654, 964
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 2 cut(s) 380, 518
HpyCH4IV ACGT 1 cut(s) 618
HpyCH4V TGCA 7 cut(s) 319, 456, 545, 581, 686, 753, 808
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 1 cut(s) 924
HpySE526I ACGT 1 cut(s) 618
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 6 cut(s) 161, 319, 323, 462, 481, 769
KspAI GTTAAC 1 cut(s) 964
Kzo9I GATC 6 cut(s) 64, 115, 367, 474, 506, 920
LmnI GCTCC 5 cut(s) 46, 629, 730, 832, 886
Lsp1109I GCAGC 1 cut(s) 565
LweI GCATC 3 cut(s) 532, 546, 817
MaeII ACGT 1 cut(s) 618
MaeIII GTNAC 1 cut(s) 569
MalI GATC 6 cut(s) 66, 117, 369, 476, 508, 922
MbiI CCGCTC 1 cut(s) 827
MboI GATC 6 cut(s) 64, 115, 367, 474, 506, 920
MboII GAAGA 1 cut(s) 239
MfeI CAATTG 1 cut(s) 110
MflI RGATCY 2 cut(s) 367, 920
MhlI GDGCHC 1 cut(s) 634
MlsI TGGCCA 3 cut(s) 5, 53, 403
MluCI AATT 8 cut(s) 110, 282, 344, 419, 486, 518, 868, 929
MluNI TGGCCA 3 cut(s) 5, 53, 403
MlyI GAGTC 1 cut(s) 166
MmeI TCCRAC 1 cut(s) 601
MnlI CCTC 5 cut(s) 54, 263, 430, 607, 738
Mox20I TGGCCA 3 cut(s) 5, 53, 403
Mph1103I ATGCAT 2 cut(s) 321, 547
MroXI GAANNNNTTC 1 cut(s) 703
MscI TGGCCA 3 cut(s) 5, 53, 403
MseI TTAA 4 cut(s) 423, 663, 963, 988
MslI CAYNNNNRTG 2 cut(s) 245, 708
Msp20I TGGCCA 3 cut(s) 5, 53, 403
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 3 cut(s) 86, 708, 838
MspR9I CCNGG 5 cut(s) 80, 86, 354, 738, 839
MunI CAATTG 1 cut(s) 110
MvaI CCWGG 3 cut(s) 80, 354, 738
MwoI GCNNNNNNNGC 1 cut(s) 542
NciI CCSGG 2 cut(s) 86, 839
NdeI CATATG 1 cut(s) 514
NdeII GATC 6 cut(s) 64, 115, 367, 474, 506, 920
NlaIII CATG 6 cut(s) 161, 319, 323, 462, 481, 769
NlaIV GGNNCC 2 cut(s) 84, 888
NsiI ATGCAT 2 cut(s) 321, 547
NspI RCATGY 3 cut(s) 319, 462, 769
NspV TTCGAA 1 cut(s) 933
PdmI GAANNNNTTC 1 cut(s) 703
PfeI GAWTC 3 cut(s) 193, 264, 797
PkrI GCNGC 1 cut(s) 580
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PpuMI RGGWCCY 2 cut(s) 82, 389
PshAI GACNNNNGTC 1 cut(s) 746
Psp124BI GAGCTC 1 cut(s) 634
Psp5II RGGWCCY 2 cut(s) 82, 389
Psp6I CCWGG 3 cut(s) 78, 352, 736
PspEI GGTNACC 1 cut(s) 569
PspFI CCCAGC 1 cut(s) 574
PspGI CCWGG 3 cut(s) 78, 352, 736
PspN4I GGNNCC 2 cut(s) 84, 888
PspPI GGNCC 4 cut(s) 76, 82, 389, 970
PspPPI RGGWCCY 2 cut(s) 82, 389
PsuI RGATCY 2 cut(s) 367, 920
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 2 cut(s) 309, 429
RsaNI GTAC 2 cut(s) 308, 428
RseI CAYNNNNRTG 2 cut(s) 245, 708
SacI GAGCTC 1 cut(s) 634
SaqAI TTAA 4 cut(s) 423, 663, 963, 988
SatI GCNGC 1 cut(s) 579
Sau3AI GATC 6 cut(s) 64, 115, 367, 474, 506, 920
Sau96I GGNCC 4 cut(s) 76, 82, 389, 970
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 5 cut(s) 80, 86, 354, 738, 839
SduI GDGCHC 1 cut(s) 634
SfaNI GCATC 3 cut(s) 532, 546, 817
SfuI TTCGAA 1 cut(s) 933
SinI GGWCC 3 cut(s) 82, 389, 970
SmiMI CAYNNNNRTG 2 cut(s) 245, 708
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
Sse9I AATT 8 cut(s) 110, 282, 344, 419, 486, 518, 868, 929
SsiI CCGC 2 cut(s) 261, 825
SspI AATATT 1 cut(s) 295
SstI GAGCTC 1 cut(s) 634
StyD4I CCNGG 5 cut(s) 78, 84, 352, 736, 837
TaiI ACGT 1 cut(s) 621
TaqI TCGA 4 cut(s) 33, 118, 782, 933
TasI AATT 8 cut(s) 110, 282, 344, 419, 486, 518, 868, 929
TfiI GAWTC 3 cut(s) 193, 264, 797
Tru1I TTAA 4 cut(s) 423, 663, 963, 988
Tru9I TTAA 4 cut(s) 423, 663, 963, 988
TscAI CASTG 3 cut(s) 504, 643, 888
TseI GCWGC 1 cut(s) 578
TspDTI ATGAA 2 cut(s) 336, 531
TspRI CASTG 3 cut(s) 504, 643, 888
VpaK11BI GGWCC 3 cut(s) 82, 389, 970
XapI RAATTY 4 cut(s) 419, 518, 868, 929
XceI RCATGY 3 cut(s) 319, 462, 769
XmnI GAANNNNTTC 1 cut(s) 703
ZraI GACGTC 1 cut(s) 619
Zsp2I ATGCAT 2 cut(s) 321, 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.