Rorug02G0134700

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005695
Physical Location & Seq
Reverse (-)
11602273 .. 11605790
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0134700.1

Sequence Viewer

Length: 843 bp
ATGTTCATTGTAGTTGTTGAATATACTGGAGCATTGAAGAGACAACAATCTGTTGCTAAAATCAATGGTTTTAAGAAATCCATAACAGTCTATCAACTTTTTAAAGAGTCTGTGGACTTTTCAAAAAGTTTGTTGAGTGGATGGATTGTTGCTGTTCCGGAACTGAAATTACTCAAGATGTTGGATGGATTCTTACTTCTTTTTCCAGAATACCTTGTTACAGTATCCAAGAACCAGATCTTGTATGTCTTTTTCAGTTCCCCTAGACTCCGGGAAACTGTTGGGACCAGGGTTTTGATGCCAATACCAGGAGGACTAATTGAGCTTCTTGTTGCCAAACAAGAAGCAGATCCTGAGGATTTAAGAGAAGGTCAGAAACGGATGATGGCATTGTTCCATGAATACGGAGTCACTCCATTTTCTCAATTGAAAGGTTTATTCATTCAAGGTCCTATTTTCATCAGATTCTTTCTTGCTAAACATGGCGAGAAAGTTCCGTCTTTTCAAAATGGTGGTGCTCTCTGGTTTACTGATCTCACAACTCCAGATAGCATGCTGATCCTTCCAATTTTGACAGCAATGACATTCTGGATCACAGTCGAGGCAGAGACCATTGTGAGGTTGAAGATGGCTTGGATTTACGGCCAGCAGAGGAATGTCTCGGCTCAGTTGCTGCGTCTCTTTTTCCATGACTGCTTCATTGAGAGGATTGGGCAAGTACTCATGCATTGGATACTTGATTCGGAGCCCAGCTCAAGTCTCGGTTCTCTATTCCTTTTTGCTGATACCAATTTACTTTATGCTGATCTCTACTTGTGCAGAGAGCATAATCAAGTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0007154 GO:0008150 GO:0009267 GO:0009605 GO:0009987 GO:0009991 GO:0031667 GO:0031668 GO:0031669 GO:0033554 GO:0042594 GO:0050896 GO:0051716 GO:0071496
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

280

Amino Acids

32.09

Weight (kDa)

7.75

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 120 - 204 5.5e-09 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 157
AclWI GGATC 3 cut(s) 344, 553, 599
AcoI YGGCCR 1 cut(s) 643
AfaI GTAC 1 cut(s) 720
AfiI CCNNNNNNNGG 2 cut(s) 308, 618
AgsI TTSAA 7 cut(s) 20, 37, 123, 430, 446, 506, 625
AjnI CCWGG 2 cut(s) 287, 307
AluBI AGCT 2 cut(s) 325, 753
AluI AGCT 2 cut(s) 325, 753
Alw21I GWGCWC 1 cut(s) 520
Alw26I GTCTC 5 cut(s) 34, 602, 664, 683, 764
AlwI GGATC 3 cut(s) 344, 553, 599
AlwNI CAGNNNCTG 2 cut(s) 353, 673
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 1 cut(s) 643
ApeKI GCWGC 1 cut(s) 673
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AspS9I GGNCC 2 cut(s) 285, 449
AsuC2I CCSGG 1 cut(s) 272
AvaII GGWCC 2 cut(s) 285, 449
AxyI CCTNAGG 1 cut(s) 354
BanII GRGCYC 1 cut(s) 750
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 1 cut(s) 660
BccI CCATC 4 cut(s) 135, 179, 379, 622
BceAI ACGGC 1 cut(s) 658
BciT130I CCWGG 2 cut(s) 289, 309
BciVI GTATCC 2 cut(s) 235, 726
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 5 cut(s) 34, 602, 664, 683, 764
BfaI CTAG 1 cut(s) 264
BfuI GTATCC 2 cut(s) 235, 726
BglII AGATCT 1 cut(s) 237
BisI GCNGC 1 cut(s) 674
BlsI GCNGC 1 cut(s) 675
BmcAI AGTACT 1 cut(s) 720
Bme1390I CCNGG 3 cut(s) 272, 289, 309
Bme18I GGWCC 2 cut(s) 285, 449
BmgT120I GGNCC 2 cut(s) 285, 449
BmiI GGNNCC 2 cut(s) 286, 747
BmrFI CCNGG 3 cut(s) 272, 289, 309
BmsI GCATC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BpmI CTGGAG 2 cut(s) 48, 528
BpuEI CTTGAG 2 cut(s) 158, 739
BpuMI CCSGG 1 cut(s) 272
BsaI GGTCTC 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 618
Bse1I ACTGG 1 cut(s) 31
Bse21I CCTNAGG 1 cut(s) 354
Bse3DI GCAATG 1 cut(s) 585
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 2 cut(s) 289, 309
BseDI CCNNGG 1 cut(s) 288
BseGI GGATG 3 cut(s) 146, 190, 387
BseLI CCNNNNNNNGG 2 cut(s) 308, 618
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 2 cut(s) 345, 680
BseNI ACTGG 1 cut(s) 31
BseXI GCAGC 1 cut(s) 660
BseYI CCCAGC 1 cut(s) 749
BsgI GTGCAG 1 cut(s) 838
BshFI GGCC 1 cut(s) 645
BsiHKAI GWGCWC 1 cut(s) 520
BsiSI CCGG 2 cut(s) 158, 271
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 2 cut(s) 308, 618
BsmAI GTCTC 5 cut(s) 34, 602, 664, 683, 764
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 1 cut(s) 298
BsnI GGCC 1 cut(s) 645
Bso31I GGTCTC 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 520, 750
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 6 cut(s) 237, 349, 532, 558, 591, 805
BspANI GGCC 1 cut(s) 645
BspCNI CTCAG 2 cut(s) 346, 679
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 286, 747
BspPI GGATC 3 cut(s) 344, 553, 599
BspTNI GGTCTC 1 cut(s) 602
BsrDI GCAATG 1 cut(s) 585
BsrI ACTGG 1 cut(s) 31
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 6 cut(s) 237, 349, 532, 558, 591, 805
Bst2UI CCWGG 2 cut(s) 289, 309
Bst4CI ACNGT 4 cut(s) 88, 223, 280, 598
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 2 cut(s) 554, 647
BstDEI CTNAG 2 cut(s) 354, 666
BstF5I GGATG 3 cut(s) 146, 190, 387
BstKTI GATC 6 cut(s) 240, 352, 535, 561, 594, 808
BstMAI GTCTC 5 cut(s) 34, 602, 664, 683, 764
BstMBI GATC 6 cut(s) 237, 349, 532, 558, 591, 805
BstNI CCWGG 2 cut(s) 289, 309
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 3 cut(s) 270, 287, 307
BstV1I GCAGC 1 cut(s) 660
BstX2I RGATCY 2 cut(s) 237, 349
BstYI RGATCY 2 cut(s) 237, 349
Bsu36I CCTNAGG 1 cut(s) 354
BsuI GTATCC 2 cut(s) 235, 726
BsuRI GGCC 1 cut(s) 645
BtsCI GGATG 3 cut(s) 146, 190, 387
Cac8I GCNNGC 2 cut(s) 554, 647
CaiI CAGNNNCTG 2 cut(s) 353, 673
Cfr13I GGNCC 2 cut(s) 285, 449
CseI GACGC 1 cut(s) 665
Csp6I GTAC 1 cut(s) 719
CviAII CATG 5 cut(s) 398, 482, 553, 689, 724
CviJI RGCY 6 cut(s) 325, 632, 645, 665, 748, 753
CviKI_1 RGCY 6 cut(s) 325, 632, 645, 665, 748, 753
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 2 cut(s) 354, 666
DpnI GATC 6 cut(s) 239, 351, 534, 560, 593, 807
DpnII GATC 6 cut(s) 237, 349, 532, 558, 591, 805
DraI TTTAAA 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 643
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco24I GRGCYC 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 602
Eco47I GGWCC 2 cut(s) 285, 449
Eco81I CCTNAGG 1 cut(s) 354
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 2 cut(s) 287, 307
EcoT22I ATGCAT 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 750
Esp3I CGTCTC 1 cut(s) 683
FaeI CATG 5 cut(s) 401, 485, 556, 692, 727
FaqI GGGAC 1 cut(s) 298
FatI CATG 5 cut(s) 397, 481, 552, 688, 723
Fnu4HI GCNGC 1 cut(s) 674
FokI GGATG 3 cut(s) 153, 197, 394
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 1 cut(s) 674
FspBI CTAG 1 cut(s) 264
GluI GCNGC 1 cut(s) 674
GsaI CCCAGC 1 cut(s) 753
GsuI CTGGAG 2 cut(s) 48, 528
HaeIII GGCC 1 cut(s) 645
HapII CCGG 2 cut(s) 158, 271
HgaI GACGC 1 cut(s) 665
Hin1II CATG 5 cut(s) 401, 485, 556, 692, 727
HinfI GANTC 6 cut(s) 107, 189, 267, 408, 465, 740
HpaII CCGG 2 cut(s) 158, 271
Hpy166II GTNNAC 3 cut(s) 115, 528, 838
Hpy188I TCNGA 3 cut(s) 375, 464, 745
Hpy188III TCNNGA 6 cut(s) 158, 175, 206, 353, 545, 589
Hpy8I GTNNAC 3 cut(s) 115, 528, 838
HpyAV CCTTC 2 cut(s) 362, 572
HpyCH4III ACNGT 4 cut(s) 88, 223, 280, 598
HpyCH4V TGCA 2 cut(s) 727, 819
HpyF3I CTNAG 2 cut(s) 354, 666
Hsp92II CATG 5 cut(s) 401, 485, 556, 692, 727
Kpn2I TCCGGA 1 cut(s) 157
Kzo9I GATC 6 cut(s) 237, 349, 532, 558, 591, 805
LmnI GCTCC 2 cut(s) 29, 745
Lsp1109I GCAGC 1 cut(s) 660
LweI GCATC 1 cut(s) 288
MaeI CTAG 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 217, 409
MalI GATC 6 cut(s) 239, 351, 534, 560, 593, 807
MboI GATC 6 cut(s) 237, 349, 532, 558, 591, 805
MboII GAAGA 2 cut(s) 49, 637
MfeI CAATTG 1 cut(s) 425
MflI RGATCY 2 cut(s) 237, 349
MhlI GDGCHC 2 cut(s) 520, 750
MluCI AATT 5 cut(s) 167, 318, 425, 567, 790
MlyI GAGTC 3 cut(s) 116, 261, 417
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 6 cut(s) 305, 349, 595, 612, 645, 699
Mph1103I ATGCAT 1 cut(s) 729
MroI TCCGGA 1 cut(s) 157
MseI TTAA 3 cut(s) 72, 102, 362
MspI CCGG 2 cut(s) 158, 271
MspR9I CCNGG 3 cut(s) 272, 289, 309
MunI CAATTG 1 cut(s) 425
MvaI CCWGG 2 cut(s) 289, 309
NciI CCSGG 1 cut(s) 272
NdeII GATC 6 cut(s) 237, 349, 532, 558, 591, 805
NlaIII CATG 5 cut(s) 401, 485, 556, 692, 727
NlaIV GGNNCC 2 cut(s) 286, 747
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 1 cut(s) 409
NsiI ATGCAT 1 cut(s) 729
NspI RCATGY 1 cut(s) 556
PaeI GCATGC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 189, 465, 740
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 1 cut(s) 675
PleI GAGTC 3 cut(s) 115, 261, 416
PpsI GAGTC 3 cut(s) 115, 261, 416
PpuMI RGGWCCY 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 287, 307
PspFI CCCAGC 1 cut(s) 749
PspGI CCWGG 2 cut(s) 287, 307
PspN4I GGNNCC 2 cut(s) 286, 747
PspPI GGNCC 2 cut(s) 285, 449
PspPPI RGGWCCY 1 cut(s) 449
PsrI GAACNNNNNNTAC 2 cut(s) 153, 185
PstNI CAGNNNCTG 2 cut(s) 353, 673
PsuI RGATCY 2 cut(s) 237, 349
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
SaqAI TTAA 3 cut(s) 72, 102, 362
SatI GCNGC 1 cut(s) 674
Sau3AI GATC 6 cut(s) 237, 349, 532, 558, 591, 805
Sau96I GGNCC 2 cut(s) 285, 449
ScaI AGTACT 1 cut(s) 720
SchI GAGTC 3 cut(s) 116, 261, 417
ScrFI CCNGG 3 cut(s) 272, 289, 309
SduI GDGCHC 2 cut(s) 520, 750
SetI ASST 7 cut(s) 216, 327, 373, 436, 451, 623, 755
SfaNI GCATC 1 cut(s) 288
SinI GGWCC 2 cut(s) 285, 449
SmlI CTYRAG 2 cut(s) 173, 754
SmoI CTYRAG 2 cut(s) 173, 754
SphI GCATGC 1 cut(s) 556
Sse9I AATT 5 cut(s) 167, 318, 425, 567, 790
SspMI CTAG 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 270, 287, 307
TaaI ACNGT 4 cut(s) 88, 223, 280, 598
TaqI TCGA 1 cut(s) 600
TasI AATT 5 cut(s) 167, 318, 425, 567, 790
TatI WGTACW 1 cut(s) 718
TfiI GAWTC 3 cut(s) 189, 465, 740
Tru1I TTAA 3 cut(s) 72, 102, 362
Tru9I TTAA 3 cut(s) 72, 102, 362
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 673
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 4 cut(s) 414, 430, 448, 688
TspGWI ACGGA 3 cut(s) 394, 420, 486
VpaK11BI GGWCC 2 cut(s) 285, 449
XceI RCATGY 1 cut(s) 556
XspI CTAG 1 cut(s) 264
ZrmI AGTACT 1 cut(s) 720
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.