AT3G50440

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009251
Physical Location & Seq
Reverse (-)
18717392 .. 18718396
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50440.1

Sequence Viewer

Length: 828 bp
ATGACATACCAAAAGCAATACCAAATGCAAACACACCACATGCAGCAACAACAACTCCATCACTTCGTCTTTGTCCACGGCTCATGTCATGGAGCATGGTGCTGGTTCAAACTAGCTGCAAAGCTCAAGCTCGATGGTCACCGTGTGACAGCTATCGACCTTGGTGGCTCCGGTGTCGACACGAGGCAGCTCCATGAGGTTCGTTTGGTGTCGGCGTATCTAGAGCCTTTGATGAGTTTCATGGAGTCTCTCCCTGAAAACGAGAAAGTGGTTCTTGTTGGTCATAGCTATGGTGGCATTGGAACTTCTCTTGCTATGGAAAGGTTTCCGACCAAAGTCTCTGTCGGAATCTTCCTCTCTGCTTACATGCCGCACCATGACTCTCCTCCGGCGGTTTTGATTCAAGAGTATTTTACGAGACTACCAGAGGGTTTCGCTATGGACTGTGAGTTTACATTCGAGGAAGGATTAGAACACCCACCAAGTTCAGTTTTGTTCGGTACTAGTTTCTTGAAGGAGAAGGCATATAGTAATTGCCAGTTAGAGGATCTAGAATTAGCCATGGCGTTGATGAAACCAAGCTGGTTATACACTAAGGAAATGGGAGGTGAAGATCTGATAACAAAGGAGAGGTATGGATCTGGGAAGAGAGTGTTTATTGTCTGTGAAGGCGACAATGTGGTTCCAGAGGAGATTCAGAAGTGGATGATTAGTAACTACGAGCCTCACGAAGTTAAGCGGATTGAAGAAGCTGGTCACATGGCTATGCTCACTAAGCCTCATGAACTTTCTCAACTGCTACAAGAAATAGCAGCGAAATATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016788 GO:0052689 GO:0080032
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

275

Amino Acids

31.36

Weight (kDa)

5.74

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 21 - 260 2.5e-19 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 22 - 264 6.5e-21 Alpha/beta hydrolase family
Hydrolase_4 PF12146 22 - 146 2.6e-11 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 177
AciI CCGC 3 cut(s) 371, 392, 739
AclWI GGATC 2 cut(s) 555, 646
AdeI CACNNNGTG 1 cut(s) 145
AfaI GTAC 1 cut(s) 502
AfiI CCNNNNNNNGG 1 cut(s) 544
AgsI TTSAA 4 cut(s) 109, 404, 514, 746
AhlI ACTAGT 1 cut(s) 503
AluBI AGCT 8 cut(s) 116, 124, 130, 152, 190, 288, 582, 752
AluI AGCT 8 cut(s) 116, 124, 130, 152, 190, 288, 582, 752
Alw26I GTCTC 3 cut(s) 252, 343, 412
AlwI GGATC 2 cut(s) 555, 646
ApeKI GCWGC 4 cut(s) 43, 116, 187, 812
ArsI GACNNNNNNTTYG 2 cut(s) 327, 359
Asp700I GAANNNNTTC 1 cut(s) 324
AsuHPI GGTGA 2 cut(s) 131, 620
BauI CACGAG 1 cut(s) 181
BbvI GCAGC 4 cut(s) 55, 103, 199, 824
BccI CCATC 2 cut(s) 66, 128
BceAI ACGGC 1 cut(s) 94
BcoDI GTCTC 3 cut(s) 252, 343, 412
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 4 cut(s) 113, 221, 504, 551
BglII AGATCT 1 cut(s) 613
BisI GCNGC 5 cut(s) 44, 117, 188, 371, 813
BlsI GCNGC 5 cut(s) 45, 118, 189, 372, 814
BmiI GGNNCC 2 cut(s) 169, 684
BoxI GACNNNNGTC 1 cut(s) 335
BpuEI CTTGAG 1 cut(s) 110
BsaJI CCNNGG 3 cut(s) 76, 160, 561
BsaWI WCCGGW 1 cut(s) 170
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse1I ACTGG 1 cut(s) 538
BseDI CCNNGG 3 cut(s) 76, 160, 561
BseGI GGATG 1 cut(s) 711
BseLI CCNNNNNNNGG 1 cut(s) 544
BseNI ACTGG 1 cut(s) 538
BseRI GAGGAG 2 cut(s) 375, 704
BseXI GCAGC 4 cut(s) 55, 103, 199, 824
BsiSI CCGG 2 cut(s) 171, 389
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 3 cut(s) 252, 343, 412
Bsp143I GATC 3 cut(s) 547, 613, 638
Bsp19I CCATGG 1 cut(s) 561
BspACI CCGC 3 cut(s) 371, 392, 739
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 2 cut(s) 169, 684
BspPI GGATC 2 cut(s) 555, 646
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 3 cut(s) 76, 160, 561
BssMI GATC 3 cut(s) 547, 613, 638
BssSI CACGAG 1 cut(s) 181
BssT1I CCWWGG 2 cut(s) 160, 561
Bst2BI CACGAG 1 cut(s) 181
Bst4CI ACNGT 2 cut(s) 143, 446
Bst6I CTCTTC 1 cut(s) 641
BstDEI CTNAG 2 cut(s) 594, 774
BstDSI CCRYGG 2 cut(s) 76, 561
BstEII GGTNACC 1 cut(s) 137
BstF5I GGATG 1 cut(s) 711
BstKTI GATC 3 cut(s) 550, 616, 641
BstMAI GTCTC 3 cut(s) 252, 343, 412
BstMBI GATC 3 cut(s) 547, 613, 638
BstMWI GCNNNNNNNGC 2 cut(s) 294, 775
BstNSI RCATGY 2 cut(s) 43, 370
BstPAI GACNNNNGTC 1 cut(s) 335
BstPI GGTNACC 1 cut(s) 137
BstV1I GCAGC 4 cut(s) 55, 103, 199, 824
BstX2I RGATCY 3 cut(s) 547, 613, 638
BstYI RGATCY 3 cut(s) 547, 613, 638
BtgI CCRYGG 2 cut(s) 76, 561
BtsCI GGATG 1 cut(s) 711
CciI TCATGA 1 cut(s) 781
Csp6I GTAC 1 cut(s) 501
CviQI GTAC 1 cut(s) 501
DdeI CTNAG 2 cut(s) 594, 774
DpnI GATC 3 cut(s) 549, 615, 640
DpnII GATC 3 cut(s) 547, 613, 638
DraIII CACNNNGTG 1 cut(s) 145
Eam1104I CTCTTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 641
Eco130I CCWWGG 2 cut(s) 160, 561
Eco91I GGTNACC 1 cut(s) 137
EcoO65I GGTNACC 1 cut(s) 137
EcoT14I CCWWGG 2 cut(s) 160, 561
ErhI CCWWGG 2 cut(s) 160, 561
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 5 cut(s) 44, 117, 188, 371, 813
FokI GGATG 1 cut(s) 718
Fsp4HI GCNGC 5 cut(s) 44, 117, 188, 371, 813
FspBI CTAG 4 cut(s) 113, 221, 504, 551
GluI GCNGC 5 cut(s) 44, 117, 188, 371, 813
HapII CCGG 2 cut(s) 171, 389
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 5 cut(s) 245, 348, 380, 400, 694
HpaII CCGG 2 cut(s) 171, 389
HphI GGTGA 2 cut(s) 131, 620
Hpy166II GTNNAC 3 cut(s) 76, 178, 453
Hpy188I TCNGA 4 cut(s) 330, 347, 618, 699
Hpy188III TCNNGA 7 cut(s) 221, 404, 511, 551, 686, 728, 782
Hpy8I GTNNAC 3 cut(s) 76, 178, 453
HpyAV CCTTC 4 cut(s) 458, 508, 514, 662
HpyCH4III ACNGT 2 cut(s) 143, 446
HpyCH4V TGCA 3 cut(s) 28, 43, 119
HpyF10VI GCNNNNNNNGC 2 cut(s) 294, 775
HpyF3I CTNAG 2 cut(s) 594, 774
Kzo9I GATC 3 cut(s) 547, 613, 638
LmnI GCTCC 3 cut(s) 92, 173, 195
Lsp1109I GCAGC 4 cut(s) 55, 103, 199, 824
MaeI CTAG 4 cut(s) 113, 221, 504, 551
MaeIII GTNAC 4 cut(s) 137, 145, 713, 755
MalI GATC 3 cut(s) 549, 615, 640
MboI GATC 3 cut(s) 547, 613, 638
MboII GAAGA 4 cut(s) 343, 623, 658, 758
MflI RGATCY 3 cut(s) 547, 613, 638
MluCI AATT 2 cut(s) 532, 554
MlyI GAGTC 2 cut(s) 254, 374
MmeI TCCRAC 2 cut(s) 325, 353
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 1 cut(s) 735
MslI CAYNNNNRTG 2 cut(s) 288, 764
MspI CCGG 2 cut(s) 171, 389
MwoI GCNNNNNNNGC 2 cut(s) 294, 775
NcoI CCATGG 1 cut(s) 561
NdeII GATC 3 cut(s) 547, 613, 638
NlaIV GGNNCC 2 cut(s) 169, 684
NmuCI GTSAC 3 cut(s) 137, 145, 755
NspI RCATGY 2 cut(s) 43, 370
PagI TCATGA 1 cut(s) 781
PcsI WCGNNNNNNNCGW 1 cut(s) 726
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 3 cut(s) 348, 400, 694
PkrI GCNGC 5 cut(s) 45, 118, 189, 372, 814
PleI GAGTC 2 cut(s) 253, 374
PpsI GAGTC 2 cut(s) 253, 374
PshAI GACNNNNGTC 1 cut(s) 335
PspEI GGTNACC 1 cut(s) 137
PspN4I GGNNCC 2 cut(s) 169, 684
PsuI RGATCY 3 cut(s) 547, 613, 638
RsaI GTAC 1 cut(s) 502
RsaNI GTAC 1 cut(s) 501
RseI CAYNNNNRTG 2 cut(s) 288, 764
SalI GTCGAC 1 cut(s) 176
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 5 cut(s) 44, 117, 188, 371, 813
Sau3AI GATC 3 cut(s) 547, 613, 638
SchI GAGTC 2 cut(s) 254, 374
SmiMI CAYNNNNRTG 2 cut(s) 288, 764
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 503
Sse9I AATT 2 cut(s) 532, 554
SsiI CCGC 3 cut(s) 371, 392, 739
SspMI CTAG 4 cut(s) 113, 221, 504, 551
StyI CCWWGG 2 cut(s) 160, 561
TaaI ACNGT 2 cut(s) 143, 446
TaqI TCGA 4 cut(s) 132, 156, 177, 459
TasI AATT 2 cut(s) 532, 554
TauI GCSGC 1 cut(s) 373
TfiI GAWTC 3 cut(s) 348, 400, 694
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TseFI GTSAC 3 cut(s) 137, 145, 755
TseI GCWGC 4 cut(s) 43, 116, 187, 812
Tsp45I GTSAC 3 cut(s) 137, 145, 755
TspDTI ATGAA 3 cut(s) 229, 587, 798
XbaI TCTAGA 2 cut(s) 220, 550
XceI RCATGY 2 cut(s) 43, 370
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 324
XspI CTAG 4 cut(s) 113, 221, 504, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.