pycom02g12930

Alpha/beta hydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009251
Physical Location & Seq
Reverse (-)
9517937 .. 9520178
2242 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g12930.5

Sequence Viewer

Length: 906 bp
ATGTCAAGTCAGATGTCGGAATACAATATCTCATTTGGAAAGAAGACGCGCCTGCCATCCATATCCTTTGGCGTCTGCTTCTGTTTGTTTCCTTTTCTCCTCATTGTTGTATACATGTTCGCACTGCTTTCTCATAAGCTACTATCAAGGAATATGGAGAATGCAAAGCACTTCGTTTTGGTTCACGGCACTTGCCATGGAGCATGGTGTTGGTACAAGCTTGTTGCCCTACTCCGACACGCCGGCCACCGCGTCACCGCGTTGGACCTCGGTGGTTCTGGGATCCATTCCAACCAGATTCATGAGGTCACTTCCATTTGGGATTATGTCCAGCCATTGATGGAGTTCTTGGGTTCTATTCCTGAAGATGAGAAGGTGATATTGGTAGGTCATAGTTATGGCGGCCTTCCTATGTCTTTAGCCATGGAGATGTTTCCTCAGAAGATTTCGGTTGCAGTTTTTGTCACAGCCTATTTGCCTCATTACACTTTCCCCCCCGGAGTCCTCATACAAGAATTCTTGAAAAGGACCCCAGCGGAGTCGTTATTGGATTGCCAGTTTACATTTGAACAAAATAACCCGGAAAATCAGTTGACTTCAGTCCTGTTTGGTTCACAATATATGGCAGCCAAATTGTACAAGCACTGCAACCCAGAGGATCTCGAATTAGCTAAAATGCTGACAAGGCCATCTGGGATGTTCTTGGAAGACATGAACAAGGAAGGGTTGCTTACAGAAGCGAAATTCGGGTCGGTAACTAGGGTTTTTGTATTGTGTGAAGAAGATGAGATAAAGAAAAAAACGAGGGGAAAATATTCTGCCGAAAATGCCCTTAACGAGATGTTGAAGCGTGTTCTCATTGTCTTTCGATTTATAGGTAAGGCAGTCAACGGAACATCCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016788 GO:0052689 GO:0080032
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

302

Amino Acids

33.99

Weight (kDa)

7.65

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 111
AccII CGCG 3 cut(s) 49, 252, 260
AciI CCGC 4 cut(s) 250, 258, 402, 536
AclWI GGATC 3 cut(s) 277, 290, 666
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 2 cut(s) 515, 743
AcuI CTGAAG 2 cut(s) 384, 582
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 215, 638
AflIII ACRYGT 1 cut(s) 114
AgsI TTSAA 3 cut(s) 523, 569, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 4 cut(s) 139, 220, 671, 903
AluI AGCT 4 cut(s) 139, 220, 671, 903
AlwI GGATC 3 cut(s) 277, 290, 666
AoxI GGCC 3 cut(s) 244, 403, 686
ApeKI GCWGC 1 cut(s) 626
ApoI RAATTY 2 cut(s) 515, 743
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
Asp700I GAANNNNTTC 1 cut(s) 814
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 265, 528
AsuC2I CCSGG 2 cut(s) 498, 581
AsuHPI GGTGA 2 cut(s) 247, 388
AvaII GGWCC 2 cut(s) 265, 528
BamHI GGATCC 1 cut(s) 282
BbsI GAAGAC 2 cut(s) 50, 714
BbvI GCAGC 1 cut(s) 638
BccI CCATC 3 cut(s) 64, 334, 697
BceAI ACGGC 1 cut(s) 202
BcnI CCSGG 2 cut(s) 498, 581
BfaI CTAG 1 cut(s) 759
BisI GCNGC 2 cut(s) 403, 627
BlsI GCNGC 2 cut(s) 404, 628
Bme1390I CCNGG 2 cut(s) 498, 581
Bme18I GGWCC 2 cut(s) 265, 528
BmgT120I GGNCC 2 cut(s) 265, 528
BmiI GGNNCC 2 cut(s) 284, 530
BmrFI CCNGG 2 cut(s) 498, 581
BoxI GACNNNNGTC 1 cut(s) 599
BpiI GAAGAC 2 cut(s) 50, 714
BpuMI CCSGG 2 cut(s) 498, 581
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 4 cut(s) 196, 268, 423, 496
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 1 cut(s) 556
BseDI CCNNGG 4 cut(s) 196, 268, 423, 496
BseGI GGATG 3 cut(s) 56, 702, 896
BseMII CTCAG 1 cut(s) 452
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 1 cut(s) 89
BseXI GCAGC 1 cut(s) 638
BseYI CCCAGC 1 cut(s) 532
Bsh1236I CGCG 3 cut(s) 49, 252, 260
BshFI GGCC 3 cut(s) 246, 405, 688
BsiSI CCGG 3 cut(s) 243, 498, 581
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 3 cut(s) 246, 405, 688
Bsp1407I TGTACA 1 cut(s) 636
Bsp143I GATC 2 cut(s) 282, 658
Bsp19I CCATGG 2 cut(s) 196, 423
BspACI CCGC 4 cut(s) 250, 258, 402, 536
BspANI GGCC 3 cut(s) 246, 405, 688
BspCNI CTCAG 1 cut(s) 451
BspFNI CGCG 3 cut(s) 49, 252, 260
BspHI TCATGA 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 284, 530
BspPI GGATC 3 cut(s) 277, 290, 666
BsrFI RCCGGY 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 636
BsrI ACTGG 1 cut(s) 556
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 4 cut(s) 196, 268, 423, 496
BssMI GATC 2 cut(s) 282, 658
BssNAI GTATAC 1 cut(s) 112
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 2 cut(s) 196, 423
Bst1107I GTATAC 1 cut(s) 112
BstACI GRCGYC 1 cut(s) 72
BstAUI TGTACA 1 cut(s) 636
BstC8I GCNNGC 2 cut(s) 53, 244
BstDEI CTNAG 1 cut(s) 438
BstDSI CCRYGG 2 cut(s) 196, 423
BstF5I GGATG 3 cut(s) 56, 702, 896
BstFNI CGCG 3 cut(s) 49, 252, 260
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 2 cut(s) 285, 661
BstMBI GATC 2 cut(s) 282, 658
BstMWI GCNNNNNNNGC 2 cut(s) 685, 827
BstNSI RCATGY 1 cut(s) 118
BstPAI GACNNNNGTC 1 cut(s) 599
BstSCI CCNGG 2 cut(s) 496, 579
BstUI CGCG 3 cut(s) 49, 252, 260
BstV1I GCAGC 1 cut(s) 638
BstV2I GAAGAC 2 cut(s) 50, 714
BstX2I RGATCY 2 cut(s) 282, 658
BstYI RGATCY 2 cut(s) 282, 658
BstZ17I GTATAC 1 cut(s) 112
BsuRI GGCC 3 cut(s) 246, 405, 688
BtgI CCRYGG 2 cut(s) 196, 423
BtsCI GGATG 3 cut(s) 56, 702, 896
BtsI GCAGTG 2 cut(s) 122, 643
BtsIMutI CAGTG 2 cut(s) 122, 643
Cac8I GCNNGC 2 cut(s) 53, 244
CciI TCATGA 1 cut(s) 301
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 2 cut(s) 265, 528
CseI GACGC 3 cut(s) 55, 61, 241
Csp6I GTAC 2 cut(s) 214, 637
CviAII CATG 6 cut(s) 115, 197, 204, 302, 424, 712
CviQI GTAC 2 cut(s) 214, 637
DdeI CTNAG 1 cut(s) 438
DpnI GATC 2 cut(s) 284, 660
DpnII GATC 2 cut(s) 282, 658
EaeI YGGCCR 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 196, 423
Eco47I GGWCC 2 cut(s) 265, 528
Eco57I CTGAAG 2 cut(s) 384, 582
EcoO109I RGGNCCY 1 cut(s) 528
EcoRI GAATTC 1 cut(s) 515
EcoT14I CCWWGG 2 cut(s) 196, 423
ErhI CCWWGG 2 cut(s) 196, 423
FaeI CATG 6 cut(s) 118, 200, 207, 305, 427, 715
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FatI CATG 6 cut(s) 114, 196, 203, 301, 423, 711
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 2 cut(s) 403, 627
FokI GGATG 3 cut(s) 43, 709, 883
Fsp4HI GCNGC 2 cut(s) 403, 627
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 50
GluI GCNGC 2 cut(s) 403, 627
GsaI CCCAGC 1 cut(s) 536
HaeIII GGCC 3 cut(s) 246, 405, 688
HapII CCGG 3 cut(s) 243, 498, 581
HgaI GACGC 3 cut(s) 55, 61, 241
HhaI GCGC 1 cut(s) 51
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 6 cut(s) 118, 200, 207, 305, 427, 715
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HincII GTYRAC 2 cut(s) 594, 889
HindII GTYRAC 2 cut(s) 594, 889
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 3 cut(s) 298, 501, 539
HpaII CCGG 3 cut(s) 243, 498, 581
HphI GGTGA 2 cut(s) 247, 388
Hpy166II GTNNAC 6 cut(s) 112, 184, 561, 594, 614, 889
Hpy188I TCNGA 4 cut(s) 12, 19, 236, 441
Hpy188III TCNNGA 4 cut(s) 302, 362, 520, 662
Hpy8I GTNNAC 6 cut(s) 112, 184, 561, 594, 614, 889
HpyAV CCTTC 3 cut(s) 367, 416, 716
HpyCH4V TGCA 3 cut(s) 164, 455, 648
HpyF10VI GCNNNNNNNGC 2 cut(s) 685, 827
HpyF3I CTNAG 1 cut(s) 438
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 6 cut(s) 118, 200, 207, 305, 427, 715
HspAI GCGC 1 cut(s) 49
KroI GCCGGC 1 cut(s) 242
KroNI GCCGGC 1 cut(s) 244
Kzo9I GATC 2 cut(s) 282, 658
LmnI GCTCC 1 cut(s) 200
Lsp1109I GCAGC 1 cut(s) 638
MaeI CTAG 1 cut(s) 759
MaeIII GTNAC 4 cut(s) 253, 307, 463, 754
MalI GATC 2 cut(s) 284, 660
MboI GATC 2 cut(s) 282, 658
MboII GAAGA 6 cut(s) 55, 377, 454, 719, 791, 794
MflI RGATCY 2 cut(s) 282, 658
MluCI AATT 4 cut(s) 515, 632, 665, 743
MlyI GAGTC 2 cut(s) 510, 548
MmeI TCCRAC 3 cut(s) 243, 259, 315
MnlI CCTC 8 cut(s) 110, 278, 298, 447, 489, 515, 649, 798
MroNI GCCGGC 1 cut(s) 242
MroXI GAANNNNTTC 1 cut(s) 814
MseI TTAA 1 cut(s) 834
MslI CAYNNNNRTG 2 cut(s) 396, 428
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 3 cut(s) 243, 498, 581
MspR9I CCNGG 2 cut(s) 498, 581
Mva1269I GAATGC 1 cut(s) 166
MvnI CGCG 3 cut(s) 49, 252, 260
MwoI GCNNNNNNNGC 2 cut(s) 685, 827
NaeI GCCGGC 1 cut(s) 244
NciI CCSGG 2 cut(s) 498, 581
NcoI CCATGG 2 cut(s) 196, 423
NdeII GATC 2 cut(s) 282, 658
NgoMIV GCCGGC 1 cut(s) 242
NlaIII CATG 6 cut(s) 118, 200, 207, 305, 427, 715
NlaIV GGNNCC 2 cut(s) 284, 530
NmuCI GTSAC 3 cut(s) 253, 307, 463
NspI RCATGY 1 cut(s) 118
PagI TCATGA 1 cut(s) 301
PciI ACATGT 1 cut(s) 114
PctI GAATGC 1 cut(s) 166
PdiI GCCGGC 1 cut(s) 244
PdmI GAANNNNTTC 1 cut(s) 814
PfeI GAWTC 1 cut(s) 298
PkrI GCNGC 2 cut(s) 404, 628
PleI GAGTC 2 cut(s) 509, 547
PpsI GAGTC 2 cut(s) 509, 547
PpuMI RGGWCCY 1 cut(s) 528
PscI ACATGT 1 cut(s) 114
PshAI GACNNNNGTC 1 cut(s) 599
Psp5II RGGWCCY 1 cut(s) 528
PspFI CCCAGC 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 284, 530
PspPI GGNCC 2 cut(s) 265, 528
PspPPI RGGWCCY 1 cut(s) 528
PsuI RGATCY 2 cut(s) 282, 658
RsaI GTAC 2 cut(s) 215, 638
RsaNI GTAC 2 cut(s) 214, 637
RseI CAYNNNNRTG 2 cut(s) 396, 428
SaqAI TTAA 1 cut(s) 834
SatI GCNGC 2 cut(s) 403, 627
Sau3AI GATC 2 cut(s) 282, 658
Sau96I GGNCC 2 cut(s) 265, 528
SchI GAGTC 2 cut(s) 510, 548
ScrFI CCNGG 2 cut(s) 498, 581
SetI ASST 9 cut(s) 141, 222, 270, 309, 378, 391, 673, 880, 905
SinI GGWCC 2 cut(s) 265, 528
SmiMI CAYNNNNRTG 2 cut(s) 396, 428
Sse9I AATT 4 cut(s) 515, 632, 665, 743
SsiI CCGC 4 cut(s) 250, 258, 402, 536
SspI AATATT 1 cut(s) 815
SspMI CTAG 1 cut(s) 759
StyD4I CCNGG 2 cut(s) 496, 579
StyI CCWWGG 2 cut(s) 196, 423
TaqI TCGA 2 cut(s) 663, 868
TasI AATT 4 cut(s) 515, 632, 665, 743
TatI WGTACW 1 cut(s) 636
TauI GCSGC 1 cut(s) 405
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 1 cut(s) 834
Tru9I TTAA 1 cut(s) 834
TscAI CASTG 2 cut(s) 129, 650
TseFI GTSAC 3 cut(s) 253, 307, 463
TseI GCWGC 1 cut(s) 626
Tsp45I GTSAC 3 cut(s) 253, 307, 463
TspDTI ATGAA 2 cut(s) 290, 728
TspGWI ACGGA 1 cut(s) 906
TspRI CASTG 2 cut(s) 129, 650
VpaK11BI GGWCC 2 cut(s) 265, 528
XapI RAATTY 2 cut(s) 515, 743
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 111
XmnI GAANNNNTTC 1 cut(s) 814
XspI CTAG 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.