AT3G50770

EF-hand domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005032
Physical Location & Seq
Forward (+)
18873911 .. 18874836
926 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G50770.1

Sequence Viewer

Length: 618 bp
ATGGCAACTCAAAAAGAGAAACCTTCCTCCAATTCTTTCAAATGGTTTTCCACCAAAACCTTAAAGTTAAACCTTAGTTTCCAAAACCGACGAAGATCACCAAAATCAAACTCTTCGTCAACCTTAAACTCTCCAAGAAGCAACAGTGATGACAACAACAACATAAAGAGTCACCAGGCCTCCAAAGAAGAGCTCCGTCAAGTCTTCAGCCATTTCGACAGCGACGGCGACGGTAAGATCTCAGCCTTTGAGCTCAGACATTACTTCGGCTCTGTCGGTGAGTATATATCTCACGAGGCAGCTCAAGAGGCGATAAACGAAGTTGACACTGACGCAGACGGGTCTCTAGGGTTTGAGGACTTTGTTGGATTGATGACAAGAAGAGATCTTTACGGTGACGGTGAAGTTGATGGCGATGGAGAGTTGAAGACAGCGTTTGAGATGTTCGAGGTGGAAAAAGGATCAGGCTGCATAACACCAAAGGGTTTGCAAAAGATGCTTGTGAAGTTAGGGGAATCAAGAACGTACGGAGAGTGTGAAGCCATGATAAAGTTTTACGATATAGATGGTAATGGAATTCTTGATTTTCATGAGTTTCGTCAAATGATGACGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_6 EF-hand_7 EF-hand_8

Protein Analysis

205

Amino Acids

23.04

Weight (kDa)

5.1

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 62 - 126 1.7e-14 EF-hand domain pair
EF-hand_1 PF00036 64 - 89 9e-07 EF hand domain
EF-hand_6 PF13405 64 - 93 1.4e-07 EF-hand domain
EF-hand_8 PF13833 155 - 204 4.4e-08 EF-hand domain pair
EF-hand_1 PF00036 178 - 204 5.2e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 469
AcsI RAATTY 1 cut(s) 576
AcuI CTGAAG 1 cut(s) 190
AfaI GTAC 1 cut(s) 527
AgsI TTSAA 2 cut(s) 40, 427
AjnI CCWGG 1 cut(s) 174
AluBI AGCT 3 cut(s) 193, 253, 302
AluI AGCT 3 cut(s) 193, 253, 302
Alw21I GWGCWC 2 cut(s) 195, 255
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 1 cut(s) 469
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 299, 468
ApoI RAATTY 1 cut(s) 576
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
AsuHPI GGTGA 5 cut(s) 90, 164, 290, 407, 413
BanII GRGCYC 2 cut(s) 195, 255
BauI CACGAG 1 cut(s) 293
BbsI GAAGAC 2 cut(s) 196, 434
Bbv12I GWGCWC 2 cut(s) 195, 255
BbvI GCAGC 2 cut(s) 311, 455
BccI CCATC 3 cut(s) 404, 410, 560
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 1 cut(s) 176
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 1 cut(s) 347
BglII AGATCT 2 cut(s) 237, 385
BisI GCNGC 2 cut(s) 300, 469
BlsI GCNGC 2 cut(s) 301, 470
Bme1390I CCNGG 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 1 cut(s) 486
BpiI GAAGAC 2 cut(s) 196, 434
BpuEI CTTGAG 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 348
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
BseBI CCWGG 1 cut(s) 176
BseMII CTCAG 2 cut(s) 255, 268
BseXI GCAGC 2 cut(s) 311, 455
BshFI GGCC 1 cut(s) 179
BsiHKAI GWGCWC 2 cut(s) 195, 255
BsiWI CGTACG 1 cut(s) 525
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 1 cut(s) 179
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 195, 255
Bsp143I GATC 4 cut(s) 95, 237, 385, 461
BspANI GGCC 1 cut(s) 179
BspCNI CTCAG 2 cut(s) 254, 267
BspHI TCATGA 1 cut(s) 589
BspPI GGATC 1 cut(s) 469
BspQI GCTCTTC 1 cut(s) 183
BspTNI GGTCTC 1 cut(s) 348
BssMI GATC 4 cut(s) 95, 237, 385, 461
BssSI CACGAG 1 cut(s) 293
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 5 cut(s) 146, 233, 395, 401, 613
Bst6I CTCTTC 3 cut(s) 118, 183, 376
BstAPI GCANNNNNTGC 1 cut(s) 496
BstDEI CTNAG 3 cut(s) 74, 241, 254
BstKTI GATC 4 cut(s) 98, 240, 388, 464
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 4 cut(s) 95, 237, 385, 461
BstMWI GCNNNNNNNGC 2 cut(s) 308, 496
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 1 cut(s) 174
BstV1I GCAGC 2 cut(s) 311, 455
BstV2I GAAGAC 2 cut(s) 196, 434
BstX2I RGATCY 2 cut(s) 237, 385
BstYI RGATCY 2 cut(s) 237, 385
BsuRI GGCC 1 cut(s) 179
BtgZI GCGATG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 151, 327
CciI TCATGA 1 cut(s) 589
CseI GACGC 1 cut(s) 341
Csp6I GTAC 1 cut(s) 526
CviAII CATG 2 cut(s) 544, 590
CviJI RGCY 9 cut(s) 179, 193, 210, 245, 253, 270, 302, 468, 542
CviKI_1 RGCY 9 cut(s) 179, 193, 210, 245, 253, 270, 302, 468, 542
CviQI GTAC 1 cut(s) 526
DdeI CTNAG 3 cut(s) 74, 241, 254
DpnI GATC 4 cut(s) 97, 239, 387, 463
DpnII GATC 4 cut(s) 95, 237, 385, 461
Eam1104I CTCTTC 3 cut(s) 118, 183, 376
EarI CTCTTC 3 cut(s) 118, 183, 376
Ecl136II GAGCTC 2 cut(s) 193, 253
Eco147I AGGCCT 1 cut(s) 179
Eco24I GRGCYC 2 cut(s) 195, 255
Eco31I GGTCTC 1 cut(s) 348
Eco53kI GAGCTC 2 cut(s) 193, 253
Eco57I CTGAAG 1 cut(s) 190
EcoICRI GAGCTC 2 cut(s) 193, 253
EcoRI GAATTC 1 cut(s) 576
EcoRII CCWGG 1 cut(s) 174
EcoT38I GRGCYC 2 cut(s) 195, 255
FaeI CATG 2 cut(s) 547, 593
FaiI YATR 7 cut(s) 164, 285, 287, 473, 545, 563, 591
FatI CATG 2 cut(s) 543, 589
Fnu4HI GCNGC 2 cut(s) 300, 469
FriOI GRGCYC 2 cut(s) 195, 255
Fsp4HI GCNGC 2 cut(s) 300, 469
FspBI CTAG 1 cut(s) 347
GluI GCNGC 2 cut(s) 300, 469
HaeIII GGCC 1 cut(s) 179
HgaI GACGC 1 cut(s) 341
Hin1II CATG 2 cut(s) 547, 593
HincII GTYRAC 2 cut(s) 120, 325
HindII GTYRAC 2 cut(s) 120, 325
HinfI GANTC 2 cut(s) 169, 515
HphI GGTGA 5 cut(s) 90, 164, 290, 407, 413
Hpy166II GTNNAC 2 cut(s) 120, 325
Hpy188I TCNGA 1 cut(s) 257
Hpy188III TCNNGA 5 cut(s) 293, 305, 519, 581, 590
Hpy8I GTNNAC 2 cut(s) 120, 325
Hpy99I CGWCG 3 cut(s) 93, 227, 233
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 5 cut(s) 146, 233, 395, 401, 613
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 2 cut(s) 471, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 496
HpyF3I CTNAG 3 cut(s) 74, 241, 254
HpySE526I ACGT 1 cut(s) 524
Hsp92II CATG 2 cut(s) 547, 593
Kzo9I GATC 4 cut(s) 95, 237, 385, 461
LguI GCTCTTC 1 cut(s) 183
LmnI GCTCC 1 cut(s) 198
LpnPI CCDG 3 cut(s) 161, 188, 450
Lsp1109I GCAGC 2 cut(s) 311, 455
LweI GCATC 1 cut(s) 486
MaeI CTAG 1 cut(s) 347
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 2 cut(s) 170, 395
MalI GATC 4 cut(s) 97, 239, 387, 463
MboI GATC 4 cut(s) 95, 237, 385, 461
MboII GAAGA 6 cut(s) 105, 105, 196, 200, 393, 439
MflI RGATCY 2 cut(s) 237, 385
MhlI GDGCHC 2 cut(s) 195, 255
MluCI AATT 2 cut(s) 31, 576
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 6 cut(s) 37, 190, 289, 301, 349, 442
MseI TTAA 3 cut(s) 62, 68, 125
MspR9I CCNGG 1 cut(s) 176
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 308, 496
NdeII GATC 4 cut(s) 95, 237, 385, 461
NlaIII CATG 2 cut(s) 547, 593
NmuCI GTSAC 2 cut(s) 170, 395
PagI TCATGA 1 cut(s) 589
PceI AGGCCT 1 cut(s) 179
PciSI GCTCTTC 1 cut(s) 183
PfeI GAWTC 1 cut(s) 515
Pfl23II CGTACG 1 cut(s) 525
PkrI GCNGC 2 cut(s) 301, 470
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
Psp124BI GAGCTC 2 cut(s) 195, 255
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspLI CGTACG 1 cut(s) 525
PsuI RGATCY 2 cut(s) 237, 385
RsaI GTAC 1 cut(s) 527
RsaNI GTAC 1 cut(s) 526
SacI GAGCTC 2 cut(s) 195, 255
SapI GCTCTTC 1 cut(s) 183
SaqAI TTAA 3 cut(s) 62, 68, 125
SatI GCNGC 2 cut(s) 300, 469
Sau3AI GATC 4 cut(s) 95, 237, 385, 461
SchI GAGTC 1 cut(s) 178
ScrFI CCNGG 1 cut(s) 176
SduI GDGCHC 2 cut(s) 195, 255
SetI ASST 9 cut(s) 25, 62, 75, 125, 195, 255, 304, 453, 527
SfaNI GCATC 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
Sse9I AATT 2 cut(s) 31, 576
SseBI AGGCCT 1 cut(s) 179
SspMI CTAG 1 cut(s) 347
SstI GAGCTC 2 cut(s) 195, 255
StuI AGGCCT 1 cut(s) 179
StyD4I CCNGG 1 cut(s) 174
TaaI ACNGT 5 cut(s) 146, 233, 395, 401, 613
TaiI ACGT 1 cut(s) 527
TaqI TCGA 2 cut(s) 216, 447
TasI AATT 2 cut(s) 31, 576
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 3 cut(s) 62, 68, 125
Tru9I TTAA 3 cut(s) 62, 68, 125
TscAI CASTG 2 cut(s) 151, 334
TseFI GTSAC 2 cut(s) 170, 395
TseI GCWGC 2 cut(s) 299, 468
Tsp45I GTSAC 2 cut(s) 170, 395
TspDTI ATGAA 1 cut(s) 578
TspGWI ACGGA 2 cut(s) 185, 543
TspRI CASTG 2 cut(s) 151, 334
XapI RAATTY 1 cut(s) 576
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.