MD08G1037100.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0005032
Physical Location & Seq
Forward (+)
2733140 .. 2733715
576 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1037100.v1.1.491

Sequence Viewer

Length: 576 bp
ATGAAAATCCTGCACAGATACTCCAAGCTCTTGAAGTGGTTCTTAAACAGTAAGAATTTCAAACTAACTCTCCTCCCTTGCCTTCTTTCAAAACCCGAATCCAAAACCGAGAGCCCTTGTTCAACTTTCCCTACTGAAAATCCAAACAATGGCATCACAAACAAAGAGCTTGCAAAAGTTTTTGATCATTTTGACACCAACAACGACGGCAAGATCTCCGGCGACGAGCTCTTCGCTTACTTCGTGTCCATCGGGGAAGCCATGTCCAACACCGAGGCTCAGAGCGTGATCGAAGAATTCGATAGCAATGGTGACAACTTGTTGGAGTTTGAAGACTTCGTGAAGTTGATGGAAAGAGAGGGCGATGGCAACGATGATCTCAAAAGGGCTTTTGAGATGTTTGAAGTTGACAAAGGTTGTGGATGCATAACCCCGAAAGGACTGCAGAACATGTTCAATCGATTGGGAGATGCCAAATCGTACGATGAATGTGTGTCCATGATTGGAGTGTTTGATCTTGATGGAAATGGAGTTCTTGATTTCCACGAATTTCGTAAGATGATGGTTTCAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_7 EF-hand_8

Protein Analysis

192

Amino Acids

21.63

Weight (kDa)

4.7

Isoelectric Point (pI)

27.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 55 - 117 1.4e-14 EF-hand domain pair
EF-hand_6 PF13405 56 - 85 1.2e-06 EF-hand domain
EF-hand_1 PF00036 56 - 81 1.7e-06 EF hand domain
EF-hand_8 PF13833 82 - 120 7.6e-06 EF-hand domain pair
EF-hand_7 PF13499 125 - 188 5.1e-06 EF-hand domain pair
EF-hand_8 PF13833 141 - 189 9.4e-06 EF-hand domain pair
EF-hand_1 PF00036 164 - 190 2.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 149
AcsI RAATTY 3 cut(s) 55, 296, 548
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 1 cut(s) 149
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 8 cut(s) 34, 61, 90, 123, 332, 404, 457, 570
AloI GAACNNNNNNTCC 2 cut(s) 516, 548
AluBI AGCT 3 cut(s) 28, 169, 229
AluI AGCT 3 cut(s) 28, 169, 229
Alw21I GWGCWC 1 cut(s) 231
ApoI RAATTY 3 cut(s) 55, 296, 548
ArsI GACNNNNNNTTYG 2 cut(s) 215, 247
Asp700I GAANNNNTTC 2 cut(s) 38, 452
AsuHPI GGTGA 1 cut(s) 323
BanII GRGCYC 2 cut(s) 116, 231
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 5 cut(s) 257, 343, 359, 515, 556
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 424, 458
BclI TGATCA 1 cut(s) 184
BfmI CTRYAG 1 cut(s) 443
BglII AGATCT 1 cut(s) 213
BmsI GCATC 3 cut(s) 162, 413, 460
BpiI GAAGAC 1 cut(s) 339
Bsa29I ATCGAT 1 cut(s) 460
BsaJI CCNNGG 1 cut(s) 273
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 54, 84
Bsc4I CCNNNNNNNGG 1 cut(s) 149
Bse3DI GCAATG 1 cut(s) 313
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 1 cut(s) 428
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMI GCAATG 1 cut(s) 313
BseMII CTCAG 1 cut(s) 293
BseRI GAGGAG 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 219
BsiWI CGTACG 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 116, 231
Bsp143I GATC 5 cut(s) 184, 213, 288, 376, 514
BspCNI CTCAG 1 cut(s) 292
BspDI ATCGAT 1 cut(s) 460
BspMAI CTGCAG 1 cut(s) 447
BspQI GCTCTTC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 5 cut(s) 184, 213, 288, 376, 514
Bst4CI ACNGT 1 cut(s) 50
Bst6I CTCTTC 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 428
BstKTI GATC 5 cut(s) 187, 216, 291, 379, 517
BstMBI GATC 5 cut(s) 184, 213, 288, 376, 514
BstNSI RCATGY 1 cut(s) 454
BstSFI CTRYAG 1 cut(s) 443
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
Bsu15I ATCGAT 1 cut(s) 460
BsuTUI ATCGAT 1 cut(s) 460
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 428
Cac8I GCNNGC 1 cut(s) 171
ClaI ATCGAT 1 cut(s) 460
Csp6I GTAC 1 cut(s) 481
CspCI CAANNNNNGTGG 2 cut(s) 400, 435
CviAII CATG 3 cut(s) 262, 451, 499
CviJI RGCY 7 cut(s) 28, 114, 169, 229, 260, 278, 389
CviKI_1 RGCY 7 cut(s) 28, 114, 169, 229, 260, 278, 389
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 1 cut(s) 279
DpnI GATC 5 cut(s) 186, 215, 290, 378, 516
DpnII GATC 5 cut(s) 184, 213, 288, 376, 514
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
Ecl136II GAGCTC 1 cut(s) 229
Eco24I GRGCYC 2 cut(s) 116, 231
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 296
EcoT22I ATGCAT 1 cut(s) 428
EcoT38I GRGCYC 2 cut(s) 116, 231
FaeI CATG 3 cut(s) 265, 454, 502
FaiI YATR 4 cut(s) 263, 428, 452, 500
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FatI CATG 3 cut(s) 261, 450, 498
FbaI TGATCA 1 cut(s) 184
FokI GGATG 1 cut(s) 435
FriOI GRGCYC 2 cut(s) 116, 231
HapII CCGG 1 cut(s) 219
Hin1II CATG 3 cut(s) 265, 454, 502
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 1 cut(s) 98
HpaII CCGG 1 cut(s) 219
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 1 cut(s) 409
Hpy188I TCNGA 1 cut(s) 282
Hpy188III TCNNGA 4 cut(s) 31, 340, 518, 536
Hpy8I GTNNAC 1 cut(s) 409
Hpy99I CGWCG 2 cut(s) 209, 227
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4V TGCA 4 cut(s) 13, 173, 426, 445
HpyF3I CTNAG 1 cut(s) 279
Hsp92II CATG 3 cut(s) 265, 454, 502
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 5 cut(s) 184, 213, 288, 376, 514
LguI GCTCTTC 1 cut(s) 236
LpnPI CCDG 2 cut(s) 23, 232
LweI GCATC 3 cut(s) 162, 413, 460
MaeIII GTNAC 1 cut(s) 311
MalI GATC 5 cut(s) 186, 215, 290, 378, 516
MboI GATC 5 cut(s) 184, 213, 288, 376, 514
MboII GAAGA 3 cut(s) 223, 305, 344
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 2 cut(s) 116, 231
MluCI AATT 3 cut(s) 55, 296, 548
MmeI TCCRAC 2 cut(s) 291, 303
MnlI CCTC 3 cut(s) 83, 268, 352
Mph1103I ATGCAT 1 cut(s) 428
MroXI GAANNNNTTC 2 cut(s) 38, 452
MseI TTAA 1 cut(s) 44
MspI CCGG 1 cut(s) 219
NdeII GATC 5 cut(s) 184, 213, 288, 376, 514
NlaIII CATG 3 cut(s) 265, 454, 502
NmuCI GTSAC 1 cut(s) 311
NsiI ATGCAT 1 cut(s) 428
NspI RCATGY 1 cut(s) 454
PciI ACATGT 1 cut(s) 450
PciSI GCTCTTC 1 cut(s) 236
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 297
PdmI GAANNNNTTC 2 cut(s) 38, 452
PfeI GAWTC 1 cut(s) 98
Pfl23II CGTACG 1 cut(s) 480
PflMI CCANNNNNTGG 1 cut(s) 149
PscI ACATGT 1 cut(s) 450
Psp124BI GAGCTC 1 cut(s) 231
PspLI CGTACG 1 cut(s) 480
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SacI GAGCTC 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 236
SaqAI TTAA 1 cut(s) 44
Sau3AI GATC 5 cut(s) 184, 213, 288, 376, 514
SduI GDGCHC 2 cut(s) 116, 231
SetI ASST 4 cut(s) 30, 171, 231, 418
SfaNI GCATC 3 cut(s) 162, 413, 460
SfcI CTRYAG 1 cut(s) 443
Sse9I AATT 3 cut(s) 55, 296, 548
SstI GAGCTC 1 cut(s) 231
TaaI ACNGT 1 cut(s) 50
TaqI TCGA 3 cut(s) 291, 300, 460
TasI AATT 3 cut(s) 55, 296, 548
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 1 cut(s) 44
Tru9I TTAA 1 cut(s) 44
TseFI GTSAC 1 cut(s) 311
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 17, 501
Van91I CCANNNNNTGG 1 cut(s) 149
XapI RAATTY 3 cut(s) 55, 296, 548
XceI RCATGY 1 cut(s) 454
XmnI GAANNNNTTC 2 cut(s) 38, 452
Zsp2I ATGCAT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.