MD15G1270300.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005032
Physical Location & Seq
Forward (+)
23460140 .. 23460721
582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1270300.v1.1.491

Sequence Viewer

Length: 582 bp
ATGGCAACAACTGATAGGCCAATTTCCAAACCCTCCAAATGGTTCTCTAACAAAACCATCAAACTCAGTTTCCCTGGTCGACGATCATCAAAATCTAGCTTCTCTACTAGTCCAAACTCTCCTTTGTCCCCAACTACTGCTCGAACCGGAATAATTAGTCCGAGATGTACAAGCAAAGAAGATGAGCTTAGAGAAGTTTTTCGCTACTTTGACGGAGATGGTGATGGAAAAATATCAGCCTCGGAGCTCAGAGCCTATTTCGGGTCCATCGGTGAGTACATGTCACACGAAGAGGCCGAGGCCGCGATCAACGATCTTGACGCGGACGGCGACAATCTGTTAGACTTTGAAGATTTTCTGCAGCTGATGAATAGCGGAGGTGGTGACGATGAGGACTTGAAGAAGGCGTTTGAGATGTTTGAGTTAGAGAAGGGAGTCATAACCCCTAAAAGCTTGCAGAGGATGCTGCACAGACTTGGTGACACCAAGTCGTACGATGAGTGTGTTACGATGATTCAAGTTTTCGATACTGATGGTAACGGGGTGCTTGATTTTAATGAGTTTCATCGAATGATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_6 EF-hand_8

Protein Analysis

194

Amino Acids

21.6

Weight (kDa)

4.75

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 60 - 123 1.7e-14 EF-hand domain pair
EF-hand_1 PF00036 62 - 87 2.4e-07 EF hand domain
EF-hand_6 PF13405 62 - 91 9.4e-08 EF-hand domain
EF-hand_5 PF13202 63 - 85 3e-07 EF hand
EF-hand_7 PF13499 131 - 192 4.1e-08 EF-hand domain pair
EF-hand_8 PF13833 143 - 192 2.1e-11 EF-hand domain pair
EF-hand_1 PF00036 168 - 192 6.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 79
AccII CGCG 2 cut(s) 305, 323
AciI CCGC 3 cut(s) 303, 323, 375
AfaI GTAC 3 cut(s) 169, 278, 494
AfiI CCNNNNNNNGG 2 cut(s) 39, 261
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 3 cut(s) 350, 400, 518
AhdI GACNNNNNGTC 1 cut(s) 487
AhlI ACTAGT 1 cut(s) 107
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 5 cut(s) 99, 187, 247, 364, 453
AluI AGCT 5 cut(s) 99, 187, 247, 364, 453
Alw21I GWGCWC 1 cut(s) 249
AoxI GGCC 3 cut(s) 17, 294, 300
ApeKI GCWGC 2 cut(s) 361, 466
Asp700I GAANNNNTTC 2 cut(s) 198, 354
AspS9I GGNCC 1 cut(s) 264
AsuHPI GGTGA 4 cut(s) 233, 284, 395, 491
AvaII GGWCC 1 cut(s) 264
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 2 cut(s) 373, 453
BccI CCATC 6 cut(s) 65, 212, 218, 275, 527, 568
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 75
BcuI ACTAGT 1 cut(s) 107
BfaI CTAG 2 cut(s) 96, 108
BfmI CTRYAG 1 cut(s) 359
BisI GCNGC 3 cut(s) 303, 362, 467
BlsI GCNGC 3 cut(s) 304, 363, 468
Bme1390I CCNGG 1 cut(s) 75
Bme18I GGWCC 1 cut(s) 264
BmeRI GACNNNNNGTC 1 cut(s) 487
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 75
BmsI GCATC 1 cut(s) 453
BsaJI CCNNGG 3 cut(s) 73, 240, 297
BsaWI WCCGGW 1 cut(s) 146
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 261
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 3 cut(s) 73, 240, 297
BseGI GGATG 1 cut(s) 468
BseLI CCNNNNNNNGG 2 cut(s) 39, 261
BseMII CTCAG 2 cut(s) 79, 262
BseXI GCAGC 2 cut(s) 373, 453
BsgI GTGCAG 1 cut(s) 452
Bsh1236I CGCG 2 cut(s) 305, 323
BshFI GGCC 3 cut(s) 19, 296, 302
BsiHKAI GWGCWC 1 cut(s) 249
BsiSI CCGG 1 cut(s) 147
BsiWI CGTACG 1 cut(s) 492
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 2 cut(s) 39, 261
BsmFI GGGAC 1 cut(s) 112
BsnI GGCC 3 cut(s) 19, 296, 302
Bsp1286I GDGCHC 1 cut(s) 249
Bsp1407I TGTACA 1 cut(s) 167
Bsp143I GATC 3 cut(s) 83, 306, 313
BspACI CCGC 3 cut(s) 303, 323, 375
BspANI GGCC 3 cut(s) 19, 296, 302
BspCNI CTCAG 2 cut(s) 78, 261
BspFNI CGCG 2 cut(s) 305, 323
BspLI GGNNCC 1 cut(s) 265
BspMAI CTGCAG 1 cut(s) 363
BsrGI TGTACA 1 cut(s) 167
BssECI CCNNGG 3 cut(s) 73, 240, 297
BssMI GATC 3 cut(s) 83, 306, 313
Bst2UI CCWGG 1 cut(s) 75
Bst6I CTCTTC 1 cut(s) 285
BstAPI GCANNNNNTGC 1 cut(s) 463
BstAUI TGTACA 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 455
BstDEI CTNAG 3 cut(s) 65, 188, 248
BstF5I GGATG 1 cut(s) 468
BstFNI CGCG 2 cut(s) 305, 323
BstKTI GATC 3 cut(s) 86, 309, 316
BstMBI GATC 3 cut(s) 83, 306, 313
BstMWI GCNNNNNNNGC 2 cut(s) 302, 463
BstNI CCWGG 1 cut(s) 75
BstNSI RCATGY 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 73
BstSFI CTRYAG 1 cut(s) 359
BstUI CGCG 2 cut(s) 305, 323
BstV1I GCAGC 2 cut(s) 373, 453
BsuRI GGCC 3 cut(s) 19, 296, 302
BtsCI GGATG 1 cut(s) 468
Cac8I GCNNGC 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 264
CseI GACGC 1 cut(s) 329
Csp6I GTAC 3 cut(s) 168, 277, 493
CviAII CATG 1 cut(s) 280
CviQI GTAC 3 cut(s) 168, 277, 493
DdeI CTNAG 3 cut(s) 65, 188, 248
DpnI GATC 3 cut(s) 85, 308, 315
DpnII GATC 3 cut(s) 83, 306, 313
DriI GACNNNNNGTC 1 cut(s) 487
Eam1104I CTCTTC 1 cut(s) 285
Eam1105I GACNNNNNGTC 1 cut(s) 487
EarI CTCTTC 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 247
EcoICRI GAGCTC 1 cut(s) 247
EcoRII CCWGG 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 1 cut(s) 283
FaiI YATR 2 cut(s) 281, 440
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 1 cut(s) 112
FatI CATG 1 cut(s) 279
FblI GTMKAC 1 cut(s) 79
Fnu4HI GCNGC 3 cut(s) 303, 362, 467
FokI GGATG 1 cut(s) 475
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 3 cut(s) 303, 362, 467
FspBI CTAG 2 cut(s) 96, 108
GluI GCNGC 3 cut(s) 303, 362, 467
HaeIII GGCC 3 cut(s) 19, 296, 302
HapII CCGG 1 cut(s) 147
HgaI GACGC 1 cut(s) 329
Hin1II CATG 1 cut(s) 283
HincII GTYRAC 1 cut(s) 80
HindII GTYRAC 1 cut(s) 80
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 2 cut(s) 435, 514
HpaII CCGG 1 cut(s) 147
HphI GGTGA 4 cut(s) 233, 284, 395, 491
Hpy166II GTNNAC 1 cut(s) 80
Hpy188I TCNGA 3 cut(s) 162, 244, 251
Hpy188III TCNNGA 1 cut(s) 317
Hpy8I GTNNAC 1 cut(s) 80
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 2 cut(s) 397, 424
HpyCH4V TGCA 3 cut(s) 361, 457, 469
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 463
HpyF3I CTNAG 3 cut(s) 65, 188, 248
Hsp92II CATG 1 cut(s) 283
Kzo9I GATC 3 cut(s) 83, 306, 313
LmnI GCTCC 1 cut(s) 244
LpnPI CCDG 3 cut(s) 60, 87, 160
Lsp1109I GCAGC 2 cut(s) 373, 453
LweI GCATC 1 cut(s) 453
MaeI CTAG 2 cut(s) 96, 108
MaeIII GTNAC 5 cut(s) 282, 383, 479, 505, 536
MalI GATC 3 cut(s) 85, 308, 315
MboI GATC 3 cut(s) 83, 306, 313
MboII GAAGA 4 cut(s) 191, 302, 362, 412
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 2 cut(s) 21, 153
MlyI GAGTC 1 cut(s) 444
MnlI CCTC 7 cut(s) 43, 250, 286, 292, 371, 385, 453
MroXI GAANNNNTTC 2 cut(s) 198, 354
MseI TTAA 2 cut(s) 555, 580
MspA1I CMGCKG 1 cut(s) 364
MspI CCGG 1 cut(s) 147
MspR9I CCNGG 1 cut(s) 75
MvaI CCWGG 1 cut(s) 75
MvnI CGCG 2 cut(s) 305, 323
MwoI GCNNNNNNNGC 2 cut(s) 302, 463
NdeII GATC 3 cut(s) 83, 306, 313
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 1 cut(s) 265
NmeAIII GCCGAG 1 cut(s) 322
NmuCI GTSAC 3 cut(s) 282, 383, 479
NspI RCATGY 1 cut(s) 283
PciI ACATGT 1 cut(s) 279
PcsI WCGNNNNNNNCGW 2 cut(s) 294, 327
PdmI GAANNNNTTC 2 cut(s) 198, 354
PfeI GAWTC 1 cut(s) 514
Pfl23II CGTACG 1 cut(s) 492
PkrI GCNGC 3 cut(s) 304, 363, 468
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
PscI ACATGT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspLI CGTACG 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 265
PspPI GGNCC 1 cut(s) 264
PstI CTGCAG 1 cut(s) 363
PvuII CAGCTG 1 cut(s) 364
RsaI GTAC 3 cut(s) 169, 278, 494
RsaNI GTAC 3 cut(s) 168, 277, 493
SacI GAGCTC 1 cut(s) 249
SalI GTCGAC 1 cut(s) 78
SaqAI TTAA 2 cut(s) 555, 580
SatI GCNGC 3 cut(s) 303, 362, 467
Sau3AI GATC 3 cut(s) 83, 306, 313
Sau96I GGNCC 1 cut(s) 264
SchI GAGTC 1 cut(s) 444
ScrFI CCNGG 1 cut(s) 75
SduI GDGCHC 1 cut(s) 249
SetI ASST 6 cut(s) 101, 189, 249, 366, 382, 455
SfaNI GCATC 1 cut(s) 453
SfcI CTRYAG 1 cut(s) 359
SinI GGWCC 1 cut(s) 264
SpeI ACTAGT 1 cut(s) 107
Sse9I AATT 2 cut(s) 21, 153
SsiI CCGC 3 cut(s) 303, 323, 375
SspMI CTAG 2 cut(s) 96, 108
SstI GAGCTC 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 73
TaqI TCGA 4 cut(s) 79, 142, 525, 568
TasI AATT 2 cut(s) 21, 153
TatI WGTACW 2 cut(s) 167, 276
TauI GCSGC 1 cut(s) 305
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 2 cut(s) 555, 580
Tru9I TTAA 2 cut(s) 555, 580
TseFI GTSAC 3 cut(s) 282, 383, 479
TseI GCWGC 2 cut(s) 361, 466
Tsp45I GTSAC 3 cut(s) 282, 383, 479
TspDTI ATGAA 2 cut(s) 383, 554
TspGWI ACGGA 1 cut(s) 228
VpaK11BI GGWCC 1 cut(s) 264
XceI RCATGY 1 cut(s) 283
XmiI GTMKAC 1 cut(s) 79
XmnI GAANNNNTTC 2 cut(s) 198, 354
XspI CTAG 2 cut(s) 96, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.