FvH4_1g14170

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005032
Physical Location & Seq
Forward (+)
7843700 .. 7845194
1495 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14170.t1

Sequence Viewer

Length: 648 bp
ATGCAGCTTCCTCCCCACCTAAAAAGTCTCTCTGAGCCAACTGATTTCTTGTACCAAACACACTGTTTCACAATGGCAGCAATAGCCGATGATGTTCAGAGAAGCTCCAAATCTTCCAAATGGTTTGCTAGCAAGAGTCTCAAGCTCAGCTTCCCACGTCGTCGATCATCGTCCAAGTCCACCTCATCGCCATCAACCTCCCCCACCTCCCCTTTCCCTACCACTCCAAGACATACCAACAGGGAAGATGAGTTCAGAGAAGTGTTTCGCTATTTCGACGGAGATGGAGATGGGAAAATCTCGGCCACGGAGCTGAGGGCTTACTTTGGTTCTATAGGGGAGTACATGTCGTATGAAGAGGCTGAGGCTGTGATCAACGAGCTCGATTCCGACGGGGACAGTCTGTTAACCTTTGGGGATTTTCTTCAGCTGATGAAGAGGGAAGGCGGTGGCGACGAGGATCTGAAGAGGGCTTTTGAGATGTTTGAACTGGAGAAAGGGTTCATAACACCAAGAAGCTTGCAGAGGATGTTGCACAGACTCGGAGACACTAAGTCCTATGATGAGTGTGCGACTATGATCAAAGTTTATGATACTGATGGGAATGGGGTGCTTGATTTTAACGAGTTTCATCGAATGATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005509 GO:0043167 GO:0043169 GO:0046872
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_6 EF-hand_8

Protein Analysis

216

Amino Acids

24.48

Weight (kDa)

5.21

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 83 - 146 2e-12 EF-hand domain pair
EF-hand_1 PF00036 84 - 109 5.5e-07 EF hand domain
EF-hand_6 PF13405 84 - 113 2.3e-07 EF-hand domain
EF-hand_5 PF13202 86 - 107 1.4e-06 EF hand
EF-hand_7 PF13499 153 - 214 1.4e-08 EF-hand domain pair
EF-hand_8 PF13833 165 - 214 1.3e-08 EF-hand domain pair
EF-hand_1 PF00036 190 - 214 9.2e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 447
AclWI GGATC 1 cut(s) 468
AcoI YGGCCR 1 cut(s) 303
AcuI CTGAAG 2 cut(s) 410, 485
AfaI GTAC 2 cut(s) 53, 344
AflIII ACRYGT 1 cut(s) 345
AgsI TTSAA 1 cut(s) 488
AhdI GACNNNNNGTC 1 cut(s) 553
AjiI CACGTC 1 cut(s) 158
AloI GAACNNNNNNTCC 4 cut(s) 236, 268, 485, 517
AluBI AGCT 8 cut(s) 7, 105, 145, 150, 313, 382, 430, 519
AluI AGCT 8 cut(s) 7, 105, 145, 150, 313, 382, 430, 519
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 3 cut(s) 32, 143, 540
AlwI GGATC 1 cut(s) 468
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 4, 77
Asp700I GAANNNNTTC 2 cut(s) 264, 500
AsuNHI GCTAGC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 384
Bbv12I GWGCWC 1 cut(s) 384
BbvCI CCTCAGC 2 cut(s) 314, 363
BbvI GCAGC 2 cut(s) 16, 89
BccI CCATC 5 cut(s) 199, 278, 284, 593, 634
BclI TGATCA 2 cut(s) 372, 579
BcoDI GTCTC 3 cut(s) 32, 143, 540
BfaI CTAG 1 cut(s) 129
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 2 cut(s) 5, 78
BlpI GCTNAGC 1 cut(s) 146
BlsI GCNGC 2 cut(s) 6, 79
BmeRI GACNNNNNGTC 1 cut(s) 553
BmgBI CACGTC 1 cut(s) 158
BmtI GCTAGC 1 cut(s) 132
BpmI CTGGAG 1 cut(s) 512
Bpu10I CCTNAGC 2 cut(s) 314, 363
Bpu1102I GCTNAGC 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 306
BsaXI ACNNNNNCTCC 4 cut(s) 332, 362, 485, 515
Bse1I ACTGG 1 cut(s) 495
BseDI CCNNGG 1 cut(s) 306
BseGI GGATG 1 cut(s) 534
BseMII CTCAG 4 cut(s) 24, 160, 305, 354
BseNI ACTGG 1 cut(s) 495
BseXI GCAGC 2 cut(s) 16, 89
BshFI GGCC 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 384
BslFI GGGAC 1 cut(s) 410
BsmAI GTCTC 3 cut(s) 32, 143, 540
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 4 cut(s) 164, 372, 460, 579
Bsp1720I GCTNAGC 1 cut(s) 146
BspACI CCGC 1 cut(s) 447
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 4 cut(s) 25, 159, 306, 355
BspOI GCTAGC 1 cut(s) 132
BspPI GGATC 1 cut(s) 468
BsrI ACTGG 1 cut(s) 495
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 4 cut(s) 164, 372, 460, 579
Bst4CI ACNGT 2 cut(s) 65, 401
Bst6I CTCTTC 3 cut(s) 351, 431, 461
BstC8I GCNNGC 2 cut(s) 130, 521
BstDEI CTNAG 5 cut(s) 33, 146, 314, 363, 552
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 534
BstKTI GATC 4 cut(s) 167, 375, 463, 582
BstMAI GTCTC 3 cut(s) 32, 143, 540
BstMBI GATC 4 cut(s) 164, 372, 460, 579
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNSI RCATGY 1 cut(s) 349
BstSFI CTRYAG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 16, 89
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 306
BtgZI GCGATG 1 cut(s) 171
BtrI CACGTC 1 cut(s) 158
BtsCI GGATG 1 cut(s) 534
BtsIMutI CAGTG 1 cut(s) 61
Cac8I GCNNGC 2 cut(s) 130, 521
Csp6I GTAC 2 cut(s) 52, 343
CviAII CATG 1 cut(s) 346
CviQI GTAC 2 cut(s) 52, 343
DdeI CTNAG 5 cut(s) 33, 146, 314, 363, 552
DpnI GATC 4 cut(s) 166, 374, 462, 581
DpnII GATC 4 cut(s) 164, 372, 460, 579
DriI GACNNNNNGTC 1 cut(s) 553
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 3 cut(s) 351, 431, 461
Eam1105I GACNNNNNGTC 1 cut(s) 553
EarI CTCTTC 3 cut(s) 351, 431, 461
Ecl136II GAGCTC 1 cut(s) 382
Eco24I GRGCYC 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 2 cut(s) 410, 485
EcoICRI GAGCTC 1 cut(s) 382
EcoT38I GRGCYC 1 cut(s) 384
FaeI CATG 1 cut(s) 349
FaiI YATR 8 cut(s) 234, 335, 347, 354, 506, 561, 578, 591
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 1 cut(s) 410
FatI CATG 1 cut(s) 345
FbaI TGATCA 2 cut(s) 372, 579
Fnu4HI GCNGC 2 cut(s) 5, 78
FokI GGATG 1 cut(s) 541
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 2 cut(s) 5, 78
FspBI CTAG 1 cut(s) 129
GluI GCNGC 2 cut(s) 5, 78
GsuI CTGGAG 1 cut(s) 512
HaeIII GGCC 1 cut(s) 305
Hin1II CATG 1 cut(s) 349
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 3 cut(s) 136, 386, 540
HpaI GTTAAC 1 cut(s) 408
Hpy166II GTNNAC 2 cut(s) 180, 408
Hpy188I TCNGA 6 cut(s) 34, 99, 257, 391, 465, 545
Hpy8I GTNNAC 2 cut(s) 180, 408
Hpy99I CGWCG 5 cut(s) 162, 165, 281, 395, 458
HpyAV CCTTC 1 cut(s) 437
HpyCH4III ACNGT 2 cut(s) 65, 401
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 3 cut(s) 4, 523, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 5 cut(s) 33, 146, 314, 363, 552
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 1 cut(s) 349
Ksp22I TGATCA 2 cut(s) 372, 579
KspAI GTTAAC 1 cut(s) 408
Kzo9I GATC 4 cut(s) 164, 372, 460, 579
LmnI GCTCC 2 cut(s) 110, 310
LpnPI CCDG 2 cut(s) 226, 476
Lsp1109I GCAGC 2 cut(s) 16, 89
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 157
MalI GATC 4 cut(s) 166, 374, 462, 581
MboI GATC 4 cut(s) 164, 372, 460, 579
MboII GAAGA 6 cut(s) 105, 257, 368, 416, 448, 478
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 384
MlyI GAGTC 2 cut(s) 145, 534
MmeI TCCRAC 1 cut(s) 414
MroXI GAANNNNTTC 2 cut(s) 264, 500
MseI TTAA 3 cut(s) 407, 621, 646
MspA1I CMGCKG 1 cut(s) 430
MwoI GCNNNNNNNGC 1 cut(s) 83
NdeII GATC 4 cut(s) 164, 372, 460, 579
NheI GCTAGC 1 cut(s) 128
NlaIII CATG 1 cut(s) 349
NmeAIII GCCGAG 1 cut(s) 281
NspI RCATGY 1 cut(s) 349
PciI ACATGT 1 cut(s) 345
PdmI GAANNNNTTC 2 cut(s) 264, 500
PfeI GAWTC 1 cut(s) 386
PkrI GCNGC 2 cut(s) 6, 79
PleI GAGTC 2 cut(s) 144, 534
PpsI GAGTC 2 cut(s) 144, 534
PscI ACATGT 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 384
PsuI RGATCY 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 430
RsaI GTAC 2 cut(s) 53, 344
RsaNI GTAC 2 cut(s) 52, 343
SacI GAGCTC 1 cut(s) 384
SaqAI TTAA 3 cut(s) 407, 621, 646
SatI GCNGC 2 cut(s) 5, 78
Sau3AI GATC 4 cut(s) 164, 372, 460, 579
SchI GAGTC 2 cut(s) 145, 534
SduI GDGCHC 1 cut(s) 384
SfcI CTRYAG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
SsiI CCGC 1 cut(s) 447
SspMI CTAG 1 cut(s) 129
SstI GAGCTC 1 cut(s) 384
TaaI ACNGT 2 cut(s) 65, 401
TaiI ACGT 1 cut(s) 160
TaqI TCGA 4 cut(s) 163, 276, 384, 634
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 3 cut(s) 407, 621, 646
Tru9I TTAA 3 cut(s) 407, 621, 646
TscAI CASTG 1 cut(s) 68
TseI GCWGC 2 cut(s) 4, 77
TspDTI ATGAA 4 cut(s) 369, 449, 493, 620
TspGWI ACGGA 2 cut(s) 294, 323
TspRI CASTG 1 cut(s) 68
XceI RCATGY 1 cut(s) 349
XmnI GAANNNNTTC 2 cut(s) 264, 500
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.