AT3G52430

pad4, atpad4 pad4 (phytoalexin deficient 4)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011011
Physical Location & Seq
Forward (+)
19431095 .. 19434450
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G52430.1

Sequence Viewer

Length: 1626 bp
ATGGACGATTGTCGATTCGAGACGAGTGAGTTGCAAGCTTCGGTAATGATATCGACTCCTTTATTTACCGATTCTTGGAGTTCATGCAACACCGCAAATTGCAACGGGAGTATAAAGATCCATGACATCGCCGGGATTACATACGTTGCTATACCGGCGGTATCGATGATTCAGTTGGGGAATCTTGTGGGCTTGCCAGTCACCGGAGATGTTCTTTTCCCCGGCTTATCCTCCGATGAACCTCTACCTATGGTCGACGCTGCCATACTCAAACTCTTTCTTCAGTTAAAGATCAAGGAAGGATTGGAATTGGAATTGTTAGGTAAAAAGCTGGTGGTGATAACCGGCCATTCAACCGGCGGCGCATTGGCCGCTTTCACCGCACTTTGGCTTCTATCTCAATCTTCTCCGCCGTCATTCCGCGTCTTTTGTATCACCTTTGGCTCTCCTCTGCTCGGAAACCAATCTCTCTCCACCTCAATTTCACGATCACGTTTAGCACACAACTTCTGCCACGTGGTCTCCATCCACGACCTCGTTCCTAGAAGCAGCAATGAACAATTCTGGCCCTTTGGAACTTACTTGTTCTGTTCCGACAAAGGAGGTGTCTGTCTAGACAACGCTGGTTCTGTTCGTCTGATGTTTAATATCCTCAACACCACAGCAACTCAAAACACCGAGGAACATCAGAGGTACGGACACTATGTGTTCACACTTTCACACATGTTTCTTAAATCTAGAAGCTTTCTTGGTGGGAGTATCCCCGACAATAGCTACCAAGCTGGTGTTGCGTTAGCCGTTGAAGCTCTAGGTTTCTCTAACGATGACACAAGTGGCGTTTTAGTCAAAGAATGTATAGAAACAGCTACAAGAATTGTTCGGGCTCCTATTCTGAGGTCAGCTGAGTTAGCCAATGAGCTTGCTAGTGTCTTGCCAGCAAGACTCGAGATTCAATGGTACAAAGATCGTTGCGATGCATCAGAAGAGCAGCTAGGTTACTACGATTTCTTCAAACGATATTCGTTGAAGAGAGACTTTAAAGTGAACATGAGTCGCATAAGACTAGCTAAGTTTTGGGACACAGTGATTAAAATGGTGGAGACGAATGAGTTACCTTTTGATTTTCATTTAGGAAAGAAATGGATTTACGCATCTCAATTTTATCAACTCTTAGCCGAGCCACTCGACATTGCGAATTTCTACAAAAACAGAGATATAAAGACTGGCGGGCATTACTTGGAGGGGAATAGACCTAAAAGGTATGAGGTGATTGATAAATGGCAGAAAGGAGTTAAAGTGCCTGAGGAGTGTGTGAGAAGCAGATACGCGAGCACAACGCAAGATACTTGCTTTTGGGCTAAGCTTGAGCAAGCAAAAGAGTGGTTGGATGAGGCGAGAAAAGAGAGTAGTGATCCCCAGAGGAGATCTTTGTTACGGGAAAAGATTGTTCCATTCGAGAGTTATGCGAATACATTGGTGACGAAGAAGGAGGTTTCTTTGGATGTTAAAGCGAAGAACTCGAGTTATAGTGTGTGGGAGGCGAATCTGAAAGAGTTCAAGTGCAAAATGGGTTATGAAAATGAAATTGAGATGGTTGTTGATGAGAGTGACGCAATGGAGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001666 GO:0002213 GO:0002376 GO:0002831 GO:0002833 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0009314 GO:0009411 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009625 GO:0009627 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009743 GO:0009751 GO:0009814 GO:0009816 GO:0009862 GO:0009863 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009967 GO:0009968 GO:0009987 GO:0010033 GO:0010104 GO:0010105 GO:0010150 GO:0010225 GO:0010310 GO:0010337 GO:0010353 GO:0010565 GO:0010618 GO:0010646 GO:0010647 GO:0010648 GO:0010817 GO:0010941 GO:0010942 GO:0014070 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0023051 GO:0023052 GO:0023056 GO:0023057 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031348 GO:0031349 GO:0032101 GO:0032103 GO:0032350 GO:0032501 GO:0032502 GO:0032870 GO:0034285 GO:0035690 GO:0036293 GO:0042221 GO:0042493 GO:0042742 GO:0042762 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043900 GO:0043902 GO:0044424 GO:0044464 GO:0045087 GO:0046677 GO:0046885 GO:0048366 GO:0048367 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048646 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051176 GO:0051193 GO:0051704 GO:0051707 GO:0051716 GO:0052318 GO:0052319 GO:0052320 GO:0052322 GO:0062012 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071322 GO:0071324 GO:0071327 GO:0071407 GO:0071446 GO:0071495 GO:0080134 GO:0080142 GO:0080151 GO:0090693 GO:0098542 GO:0099402 GO:1900367 GO:1900376 GO:1900378 GO:1900424 GO:1900426 GO:1901182 GO:1901183 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000022 GO:2000031 GO:2000068 GO:2000377 GO:2001023 GO:2001025 GO:2001038 GO:2001040
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

541

Amino Acids

60.98

Weight (kDa)

6.01

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 97 - 186 1.5e-19 Lipase (class 3)
EDS1_EP PF18117 316 - 527 2.4e-61 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52430
fragaria_vesca FvH4_1g24940 FvH4_1g24940 FvH4_1g24940
malus_domestica MD15G1136300.v1.1
prunus_persica Prupe.4G276500_v2.0.a1 Prupe.4G276500_v2.0.a1
pyrus_communis pycom15g12290
rosa_chinensis RchiOBHm_Chr2g0122591
rosa_laevigata RLG00000018693
rosa_multiflora Rmu_sc0003133.1_g000001 Rmu_sc0010907.1_g000001
rosa_roxburghii Rroxscaffold_2G00121450
rosa_rugosa Rorug02G0239700 Rorug02G0239800
rosa_samantha Rh2AG298400 Rh2BG307100 Rh2CG285200 Rh2DG322000
rosa_wichuraiana Rw2G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 255
AccII CGCG 2 cut(s) 423, 1328
AciI CCGC 8 cut(s) 93, 158, 360, 372, 381, 410, 421, 1227
AclWI GGATC 2 cut(s) 112, 1406
AcoI YGGCCR 2 cut(s) 346, 369
AcsI RAATTY 1 cut(s) 1195
AcuI CTGAAG 1 cut(s) 266
AcvI CACGTG 1 cut(s) 517
AdeI CACNNNGTG 1 cut(s) 706
AfaI GTAC 2 cut(s) 695, 959
AfiI CCNNNNNNNGG 4 cut(s) 75, 203, 387, 455
AflIII ACRYGT 1 cut(s) 723
AgsI TTSAA 6 cut(s) 354, 803, 953, 1012, 1027, 1558
Alw21I GWGCWC 1 cut(s) 1334
Alw26I GTCTC 5 cut(s) 14, 526, 1026, 1094, 1613
AlwI GGATC 2 cut(s) 112, 1406
Ama87I CYCGRG 2 cut(s) 944, 1519
AoxI GGCC 3 cut(s) 346, 369, 566
ApeKI GCWGC 3 cut(s) 260, 549, 988
ApoI RAATTY 1 cut(s) 1195
ArsI GACNNNNNNTTYG 2 cut(s) 591, 623
Asp700I GAANNNNTTC 1 cut(s) 1553
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 567
AsuC2I CCSGG 2 cut(s) 133, 222
AsuHPI GGTGA 6 cut(s) 193, 349, 370, 427, 1279, 1489
AvaI CYCGRG 2 cut(s) 944, 1519
AxyI CCTNAGG 1 cut(s) 1302
BanII GRGCYC 1 cut(s) 886
BbrPI CACGTG 1 cut(s) 517
Bbv12I GWGCWC 1 cut(s) 1334
BbvI GCAGC 3 cut(s) 247, 561, 1000
BccI CCATC 2 cut(s) 533, 1585
BceAI ACGGC 2 cut(s) 397, 782
BcgI CGANNNNNNTGC 4 cut(s) 13, 47, 1445, 1479
BciVI GTATCC 1 cut(s) 770
BcnI CCSGG 2 cut(s) 133, 222
BcoDI GTCTC 5 cut(s) 14, 526, 1026, 1094, 1613
BfaI CTAG 7 cut(s) 543, 614, 738, 809, 924, 992, 1064
BfuI GTATCC 1 cut(s) 770
BglII AGATCT 1 cut(s) 1424
BisI GCNGC 5 cut(s) 261, 361, 372, 550, 989
BlpI GCTNAGC 1 cut(s) 1359
BlsI GCNGC 5 cut(s) 262, 362, 373, 551, 990
Bme1390I CCNGG 2 cut(s) 133, 222
BmeT110I CYCGRG 2 cut(s) 944, 1519
BmgT120I GGNCC 1 cut(s) 567
BmiI GGNNCC 1 cut(s) 885
BmrFI CCNGG 2 cut(s) 133, 222
BmsI GCATC 3 cut(s) 964, 986, 1160
BoxI GACNNNNGTC 1 cut(s) 9
Bpu1102I GCTNAGC 1 cut(s) 1359
BpuEI CTTGAG 1 cut(s) 1385
BpuMI CCSGG 2 cut(s) 133, 222
Bsa29I ATCGAT 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 517
BsaI GGTCTC 1 cut(s) 526
BsaJI CCNNGG 2 cut(s) 220, 678
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 4 cut(s) 506, 536, 1281, 1311
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 203, 387, 455
Bse118I RCCGGY 3 cut(s) 154, 344, 356
Bse1I ACTGG 2 cut(s) 197, 1228
Bse21I CCTNAGG 1 cut(s) 1302
Bse3DI GCAATG 3 cut(s) 559, 1188, 1620
BseCI ATCGAT 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 220, 678
BseGI GGATG 3 cut(s) 525, 1393, 1507
BseLI CCNNNNNNNGG 4 cut(s) 75, 203, 387, 455
BseMI GCAATG 3 cut(s) 559, 1188, 1620
BseMII CTCAG 3 cut(s) 884, 894, 1293
BseNI ACTGG 2 cut(s) 197, 1228
BseRI GAGGAG 3 cut(s) 438, 1319, 1435
BseXI GCAGC 3 cut(s) 247, 561, 1000
Bsh1236I CGCG 2 cut(s) 423, 1328
BshFI GGCC 3 cut(s) 348, 371, 568
BshVI ATCGAT 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 1334
BsiHKCI CYCGRG 2 cut(s) 944, 1519
BsiSI CCGG 6 cut(s) 132, 155, 204, 222, 345, 357
BslFI GGGAC 1 cut(s) 1091
BslI CCNNNNNNNGG 4 cut(s) 75, 203, 387, 455
BsmAI GTCTC 5 cut(s) 14, 526, 1026, 1094, 1613
BsmBI CGTCTC 2 cut(s) 14, 1094
BsmFI GGGAC 1 cut(s) 1091
BsnI GGCC 3 cut(s) 348, 371, 568
Bso31I GGTCTC 1 cut(s) 526
BsoBI CYCGRG 2 cut(s) 944, 1519
Bsp1286I GDGCHC 2 cut(s) 886, 1334
Bsp143I GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
Bsp1720I GCTNAGC 1 cut(s) 1359
BspACI CCGC 8 cut(s) 93, 158, 360, 372, 381, 410, 421, 1227
BspANI GGCC 3 cut(s) 348, 371, 568
BspCNI CTCAG 3 cut(s) 885, 895, 1294
BspDI ATCGAT 1 cut(s) 164
BspFNI CGCG 2 cut(s) 423, 1328
BspLI GGNNCC 1 cut(s) 885
BspPI GGATC 2 cut(s) 112, 1406
BspQI GCTCTTC 1 cut(s) 978
BspTNI GGTCTC 1 cut(s) 526
BsrDI GCAATG 3 cut(s) 559, 1188, 1620
BsrFI RCCGGY 3 cut(s) 154, 344, 356
BsrI ACTGG 2 cut(s) 197, 1228
BssAI RCCGGY 3 cut(s) 154, 344, 356
BssECI CCNNGG 2 cut(s) 220, 678
BssMI GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
Bst4CI ACNGT 1 cut(s) 1084
Bst6I CTCTTC 2 cut(s) 978, 1022
BstBAI YACGTR 1 cut(s) 517
BstC8I GCNNGC 7 cut(s) 36, 194, 921, 936, 1229, 1330, 1371
BstDEI CTNAG 7 cut(s) 893, 903, 1068, 1171, 1302, 1359, 1623
BstF5I GGATG 3 cut(s) 525, 1393, 1507
BstFNI CGCG 2 cut(s) 423, 1328
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 6 cut(s) 120, 294, 491, 967, 1414, 1427
BstMAI GTCTC 5 cut(s) 14, 526, 1026, 1094, 1613
BstMBI GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
BstMWI GCNNNNNNNGC 6 cut(s) 155, 371, 380, 788, 803, 908
BstNSI RCATGY 1 cut(s) 727
BstPAI GACNNNNGTC 1 cut(s) 9
BstSCI CCNGG 2 cut(s) 131, 220
BstUI CGCG 2 cut(s) 423, 1328
BstV1I GCAGC 3 cut(s) 247, 561, 1000
BstX2I RGATCY 2 cut(s) 117, 1424
BstYI RGATCY 2 cut(s) 117, 1424
Bsu15I ATCGAT 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 1302
BsuI GTATCC 1 cut(s) 770
BsuRI GGCC 3 cut(s) 348, 371, 568
BsuTUI ATCGAT 1 cut(s) 164
BtgZI GCGATG 2 cut(s) 112, 987
BtsCI GGATG 3 cut(s) 525, 1393, 1507
BtsIMutI CAGTG 1 cut(s) 1089
Cac8I GCNNGC 7 cut(s) 36, 194, 921, 936, 1229, 1330, 1371
CfoI GCGC 1 cut(s) 365
Cfr10I RCCGGY 3 cut(s) 154, 344, 356
Cfr13I GGNCC 1 cut(s) 567
ClaI ATCGAT 1 cut(s) 164
CseI GACGC 3 cut(s) 266, 412, 1619
Csp6I GTAC 2 cut(s) 694, 958
CviAII CATG 4 cut(s) 84, 122, 724, 1048
CviQI GTAC 2 cut(s) 694, 958
DdeI CTNAG 7 cut(s) 893, 903, 1068, 1171, 1302, 1359, 1623
DpnI GATC 6 cut(s) 119, 293, 490, 966, 1413, 1426
DpnII GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
DraI TTTAAA 1 cut(s) 1039
DraIII CACNNNGTG 1 cut(s) 706
EaeI YGGCCR 2 cut(s) 346, 369
Eam1104I CTCTTC 2 cut(s) 978, 1022
EarI CTCTTC 2 cut(s) 978, 1022
EciI GGCGGA 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 886
Eco31I GGTCTC 1 cut(s) 526
Eco32I GATATC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 266
Eco72I CACGTG 1 cut(s) 517
Eco81I CCTNAGG 1 cut(s) 1302
Eco88I CYCGRG 2 cut(s) 944, 1519
EcoRV GATATC 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 979
EcoT38I GRGCYC 1 cut(s) 886
Esp3I CGTCTC 2 cut(s) 14, 1094
FaeI CATG 4 cut(s) 87, 125, 727, 1051
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 1 cut(s) 1091
FatI CATG 4 cut(s) 83, 121, 723, 1047
FauI CCCGC 1 cut(s) 1220
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 5 cut(s) 261, 361, 372, 550, 989
FokI GGATG 3 cut(s) 512, 1400, 1514
FriOI GRGCYC 1 cut(s) 886
Fsp4HI GCNGC 5 cut(s) 261, 361, 372, 550, 989
FspBI CTAG 7 cut(s) 543, 614, 738, 809, 924, 992, 1064
GlaI GCGC 1 cut(s) 364
GluI GCNGC 5 cut(s) 261, 361, 372, 550, 989
HaeIII GGCC 3 cut(s) 348, 371, 568
HapII CCGG 6 cut(s) 132, 155, 204, 222, 345, 357
HgaI GACGC 3 cut(s) 266, 412, 1619
HhaI GCGC 1 cut(s) 365
Hin1II CATG 4 cut(s) 87, 125, 727, 1051
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 3 cut(s) 36, 742, 1361
HinfI GANTC 9 cut(s) 15, 55, 71, 169, 181, 942, 949, 1051, 1543
HpaII CCGG 6 cut(s) 132, 155, 204, 222, 345, 357
HphI GGTGA 6 cut(s) 193, 349, 370, 427, 1279, 1489
Hpy166II GTNNAC 3 cut(s) 256, 711, 1045
Hpy188I TCNGA 8 cut(s) 235, 458, 595, 639, 690, 894, 982, 1548
Hpy188III TCNNGA 6 cut(s) 19, 486, 614, 738, 946, 1456
Hpy8I GTNNAC 3 cut(s) 256, 711, 1045
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 2 cut(s) 293, 1480
HpyCH4III ACNGT 1 cut(s) 1084
HpyCH4IV ACGT 3 cut(s) 144, 493, 516
HpyCH4V TGCA 5 cut(s) 34, 87, 102, 977, 1563
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 371, 380, 788, 803, 908
HpyF3I CTNAG 7 cut(s) 893, 903, 1068, 1171, 1302, 1359, 1623
HpySE526I ACGT 3 cut(s) 144, 493, 516
Hsp92II CATG 4 cut(s) 87, 125, 727, 1051
HspAI GCGC 1 cut(s) 363
Kzo9I GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
LguI GCTCTTC 1 cut(s) 978
LmnI GCTCC 1 cut(s) 889
Lsp1109I GCAGC 3 cut(s) 247, 561, 1000
LweI GCATC 3 cut(s) 964, 986, 1160
MaeI CTAG 7 cut(s) 543, 614, 738, 809, 924, 992, 1064
MaeII ACGT 3 cut(s) 144, 493, 516
MaeIII GTNAC 6 cut(s) 199, 995, 1110, 1431, 1477, 1607
MalI GATC 6 cut(s) 119, 293, 490, 966, 1413, 1426
MboI GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
MboII GAAGA 7 cut(s) 272, 396, 995, 1000, 1039, 1495, 1525
MflI RGATCY 2 cut(s) 117, 1424
MhlI GDGCHC 2 cut(s) 886, 1334
MluCI AATT 9 cut(s) 97, 308, 314, 480, 560, 873, 1157, 1195, 1584
MlyI GAGTC 3 cut(s) 49, 936, 1060
MmeI TCCRAC 2 cut(s) 618, 1365
Mph1103I ATGCAT 1 cut(s) 979
MroXI GAANNNNTTC 1 cut(s) 1553
MseI TTAA 7 cut(s) 287, 645, 732, 1038, 1089, 1293, 1506
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 6 cut(s) 132, 155, 204, 222, 345, 357
MspR9I CCNGG 2 cut(s) 133, 222
MvnI CGCG 2 cut(s) 423, 1328
MwoI GCNNNNNNNGC 6 cut(s) 155, 371, 380, 788, 803, 908
NciI CCSGG 2 cut(s) 133, 222
NdeII GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
NlaIII CATG 4 cut(s) 87, 125, 727, 1051
NlaIV GGNNCC 1 cut(s) 885
NmeAIII GCCGAG 1 cut(s) 1201
NmuCI GTSAC 3 cut(s) 199, 1477, 1607
NsiI ATGCAT 1 cut(s) 979
NspI RCATGY 1 cut(s) 727
PaeR7I CTCGAG 2 cut(s) 944, 1519
PciI ACATGT 1 cut(s) 723
PciSI GCTCTTC 1 cut(s) 978
PdmI GAANNNNTTC 1 cut(s) 1553
PfeI GAWTC 6 cut(s) 15, 71, 169, 181, 949, 1543
PkrI GCNGC 5 cut(s) 262, 362, 373, 551, 990
PleI GAGTC 3 cut(s) 49, 936, 1059
PmaCI CACGTG 1 cut(s) 517
PmlI CACGTG 1 cut(s) 517
PpsI GAGTC 3 cut(s) 49, 936, 1059
Ppu21I YACGTR 1 cut(s) 517
PscI ACATGT 1 cut(s) 723
PshAI GACNNNNGTC 1 cut(s) 9
PspCI CACGTG 1 cut(s) 517
PspN4I GGNNCC 1 cut(s) 885
PspPI GGNCC 1 cut(s) 567
PspXI VCTCGAGB 1 cut(s) 1519
PsuI RGATCY 2 cut(s) 117, 1424
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 2 cut(s) 695, 959
RsaNI GTAC 2 cut(s) 694, 958
SalI GTCGAC 1 cut(s) 254
SapI GCTCTTC 1 cut(s) 978
SaqAI TTAA 7 cut(s) 287, 645, 732, 1038, 1089, 1293, 1506
SatI GCNGC 5 cut(s) 261, 361, 372, 550, 989
Sau3AI GATC 6 cut(s) 117, 291, 488, 964, 1411, 1424
Sau96I GGNCC 1 cut(s) 567
SchI GAGTC 3 cut(s) 49, 936, 1060
ScrFI CCNGG 2 cut(s) 133, 222
SduI GDGCHC 2 cut(s) 886, 1334
SfaNI GCATC 3 cut(s) 964, 986, 1160
Sfr274I CTCGAG 2 cut(s) 944, 1519
SlaI CTCGAG 2 cut(s) 944, 1519
SmlI CTYRAG 3 cut(s) 944, 1364, 1519
SmoI CTYRAG 3 cut(s) 944, 1364, 1519
Sse9I AATT 9 cut(s) 97, 308, 314, 480, 560, 873, 1157, 1195, 1584
SsiI CCGC 8 cut(s) 93, 158, 360, 372, 381, 410, 421, 1227
SspMI CTAG 7 cut(s) 543, 614, 738, 809, 924, 992, 1064
StyD4I CCNGG 2 cut(s) 131, 220
TaaI ACNGT 1 cut(s) 1084
TaiI ACGT 3 cut(s) 147, 496, 519
TaqI TCGA 9 cut(s) 13, 18, 53, 164, 255, 945, 1185, 1455, 1520
TasI AATT 9 cut(s) 97, 308, 314, 480, 560, 873, 1157, 1195, 1584
TauI GCSGC 2 cut(s) 363, 374
TfiI GAWTC 6 cut(s) 15, 71, 169, 181, 949, 1543
Tru1I TTAA 7 cut(s) 287, 645, 732, 1038, 1089, 1293, 1506
Tru9I TTAA 7 cut(s) 287, 645, 732, 1038, 1089, 1293, 1506
TscAI CASTG 1 cut(s) 1089
TseFI GTSAC 3 cut(s) 199, 1477, 1607
TseI GCWGC 3 cut(s) 260, 549, 988
Tsp45I GTSAC 3 cut(s) 199, 1477, 1607
TspDTI ATGAA 6 cut(s) 72, 252, 570, 1115, 1590, 1596
TspGWI ACGGA 1 cut(s) 711
TspRI CASTG 1 cut(s) 1089
XapI RAATTY 1 cut(s) 1195
XbaI TCTAGA 2 cut(s) 613, 737
XceI RCATGY 1 cut(s) 727
XhoI CTCGAG 2 cut(s) 944, 1519
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 1553
XspI CTAG 7 cut(s) 543, 614, 738, 809, 924, 992, 1064
Zsp2I ATGCAT 1 cut(s) 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.