MD15G1136300.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011011
Physical Location & Seq
Forward (+)
9950517 .. 9959971
9455 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1136300.v1.1.491

Sequence Viewer

Length: 1899 bp
ATGGATGCTGAAGCTTCACCGTTTGAGAGCAGTGAGACAGTTGCGAGTTTCTTGGCATCGACTCCCTTGCTTATTGAGTCATGGAGGCTATGCAGCGTAGCAAACACCACTCCCGGCACCGCTTTCGAATCCCAACAAATCGGAGACGTCGGATATTTTGCTTTATCCGGAGGAGAAGAATGTTTGAGTTTCAGAAATTTGGTAGCGTTGGAGACCGCCGGCCACGGCCTTTTCCCACCATTATCCAACAACAACAATAATAATCATAATAATATTATTAATGGAGAGGAAGAGGCTGTCATGGTGCATGCAGGGCTGCTCAAGCTCTTCGTGTCCCTACGCTGCTCCCAAACGTTCCAAGACCAGGTGTCGGCCCTAATAGAGAAAAGCAAGTCCATTGTGATCACAGGCCATTCTATAGGAGGAACAACTGCCTCACTCTGTGCTCTCTGGCTACTTTCTTACCTCCGATCCATCTCTTCCACCCTTCCAGTCCTTTGCATTACCTTCGGATCACCATTGCTCGGCAATACCTCCCTTTCGCGAGCAATCCTCCGAGAAAGATGGGGTGGCAATTTTTTCCACGTAGTTTCCAAGTACGACATCATGCCAAGACTACTCCTTGCTCCAATTGAATCATGCAGCACTCAAGTACTGCATTTCTTACTACAATGCTTTCACCGCTTTCGAAATATCGGTGTTCATGTTCAACTGCGCGACGAAGAGAAAGCTGCACTATCCAGCTTCGTGTCAGCTTATTTGCGTGCGTCATCATCGCAAGAAGGAGAAGAGAGAGAAAGCAGCAGCATGTCGTATTGTCCATTTGGGAGCTACTTGTTTTGCTCACAAGAGGGAGCTGTGTGTGTGGAGAATGCAGAGTCTGTGATGACAATGATGCATTTGATGTTTATGCCAGCTGAAGCTAACCCAATGTGCTGTATCCAGGATCATCTCAACTACGGAGATTATGTTGACAAGATTTCTTCACAGTTTCTGAAGACGAGCTTCATGCAGCAGGATGAGCTTCCAGAATCAAGCTACGAAGCAGGGGTTGCATTGGCATTACAGTCCTTGGGAATAAATAGCCACGAACCGGCTGCCGCACCTGCGAAGGAGTGCCTAAAGATAGCAAGGCGATTAGGACGTACCCAAAGTTTGAATTGTGCTAATTTAGCTGTTCGGCTCTCCAAGTACACGCCTCACAGAGCTGAAATCGAGTGGTACATGGCATCCTGCAACGAGTCTGGTGAGCAACTGGGGTACTATGACGCCTTCAAGCAAAGGGACTCCTCAAAAAAGGGTAACAGGGTCAACATGAACCGCCACATGCTTGCTGCATTTTGGGATGGGGTGATAAAAATGATGGAGACAAATGAGTTGCCTCACGATTTTCACAGGCGAGCAAAATGGGTAAATGCTTCCCAGTTTTACAAACTCCTCGTCGAGCCATTGGACATCGCGGAGTACTATAGAACTGGTATGCACCGTGTCAAGGGTCATTACTTGGCGCATGGAAGAGAGAGGAGGTTTGAGATATTCGACAGATGGTGGAAGGAGATAAGCATCAACGATCAGAAGCAAAGTAGAAGCAGAAGCAAGTTTGCAGGTTTGACTCAGGATTCGTGCTTCTGGGCAAAGGTGGAGGAAGCTAGGGAATGGCTTACTAATTTTAGAAGTGAGACTGATACAAGCAACCTACATTTTCTGTGGGAAAATATCAATAAATTTGACAGCCATGCAACCAGTTTGGTCCAGAGAAAGGAGGTCTCTAAAGACGTTCTGGCAAAGAACTCAAGCTACATGAAATGGCAGCAAGAGTTTGCAGATCTGAGATCGCAGGTACGCCACGTCCAGCTTCAAATTCCAAGTCTTAGAGATGGGTCTTATTTACTTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

633

Amino Acids

71.52

Weight (kDa)

6.41

Isoelectric Point (pI)

60.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 93 - 207 2.4e-19 Lipase (class 3)
EDS1_EP PF18117 403 - 614 1.6e-70 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52430
fragaria_vesca FvH4_1g24940 FvH4_1g24940 FvH4_1g24940
malus_domestica MD15G1136300.v1.1
prunus_persica Prupe.4G276500_v2.0.a1 Prupe.4G276500_v2.0.a1
pyrus_communis pycom15g12290
rosa_chinensis RchiOBHm_Chr2g0122591
rosa_laevigata RLG00000018693
rosa_multiflora Rmu_sc0003133.1_g000001 Rmu_sc0010907.1_g000001
rosa_roxburghii Rroxscaffold_2G00121450
rosa_rugosa Rorug02G0239700 Rorug02G0239800
rosa_samantha Rh2AG298400 Rh2BG307100 Rh2CG285200 Rh2DG322000
rosa_wichuraiana Rw2G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1114
AatII GACGTC 1 cut(s) 150
Acc36I ACCTGC 3 cut(s) 1114, 1595, 1828
AccB1I GGYRCC 1 cut(s) 116
AccII CGCG 3 cut(s) 544, 717, 1460
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 6 cut(s) 120, 216, 682, 1101, 1321, 1460
AclI AACGTT 1 cut(s) 353
AclWI GGATC 3 cut(s) 465, 520, 954
AcoI YGGCCR 1 cut(s) 220
AcsI RAATTY 3 cut(s) 196, 1724, 1860
AcuI CTGAAG 3 cut(s) 30, 939, 1016
AcyI GRCGYC 2 cut(s) 147, 1269
AdeI CACNNNGTG 1 cut(s) 443
AfaI GTAC 8 cut(s) 599, 654, 1147, 1193, 1223, 1262, 1466, 1842
AfiI CCNNNNNNNGG 6 cut(s) 364, 370, 524, 1093, 1492, 1759
AgsI TTSAA 5 cut(s) 635, 710, 1159, 1276, 1859
AhdI GACNNNNNGTC 1 cut(s) 367
AjiI CACGTC 1 cut(s) 1849
AjnI CCWGG 2 cut(s) 363, 942
Alw21I GWGCWC 1 cut(s) 448
Alw26I GTCTC 6 cut(s) 29, 138, 206, 1361, 1673, 1771
AlwI GGATC 3 cut(s) 465, 520, 954
AlwNI CAGNNNCTG 2 cut(s) 881, 994
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 4 cut(s) 220, 226, 372, 409
ApoI RAATTY 3 cut(s) 196, 1724, 1860
ArsI GACNNNNNNTTYG 6 cut(s) 1425, 1457, 1603, 1635, 1852, 1884
AseI ATTAAT 1 cut(s) 279
AspLEI GCGC 2 cut(s) 717, 1510
AspS9I GGNCC 2 cut(s) 373, 1750
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 5 cut(s) 9, 507, 671, 1259, 1363
AsuII TTCGAA 2 cut(s) 126, 688
AvaII GGWCC 1 cut(s) 1750
BanI GGYRCC 1 cut(s) 116
BbsI GAAGAC 1 cut(s) 1004
Bbv12I GWGCWC 1 cut(s) 448
BccI CCATC 6 cut(s) 482, 558, 1340, 1357, 1539, 1871
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 2 cut(s) 365, 944
BciVI GTATCC 1 cut(s) 950
BclI TGATCA 1 cut(s) 402
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 6 cut(s) 29, 138, 206, 1361, 1673, 1771
BfaI CTAG 2 cut(s) 1650, 1897
BfmI CTRYAG 2 cut(s) 417, 1468
BfuAI ACCTGC 3 cut(s) 1114, 1595, 1828
BfuI GTATCC 1 cut(s) 950
BglII AGATCT 1 cut(s) 1825
BmcAI AGTACT 2 cut(s) 654, 1466
Bme1390I CCNGG 3 cut(s) 114, 365, 944
Bme18I GGWCC 1 cut(s) 1750
BmeRI GACNNNNNGTC 1 cut(s) 367
BmgBI CACGTC 1 cut(s) 1849
BmgT120I GGNCC 2 cut(s) 373, 1750
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 3 cut(s) 114, 365, 944
BmrI ACTGGG 2 cut(s) 1265, 1417
BmsI GCATC 4 cut(s) 65, 885, 1238, 1572
BmuI ACTGGG 2 cut(s) 1265, 1417
BpiI GAAGAC 1 cut(s) 1004
BplI GAGNNNNNCTC 2 cut(s) 537, 569
Bpu14I TTCGAA 2 cut(s) 126, 688
BpuEI CTTGAG 3 cut(s) 305, 633, 1777
BpuMI CCSGG 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 586
BsaBI GATNNNNATC 1 cut(s) 1562
BsaHI GRCGYC 2 cut(s) 147, 1269
BsaI GGTCTC 2 cut(s) 206, 1771
BsaJI CCNNGG 2 cut(s) 223, 1071
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 954, 984
Bsc4I CCNNNNNNNGG 6 cut(s) 364, 370, 524, 1093, 1492, 1759
Bse118I RCCGGY 2 cut(s) 218, 1093
Bse1I ACTGG 5 cut(s) 491, 1260, 1423, 1480, 1743
Bse3DI GCAATG 1 cut(s) 518
Bse8I GATNNNNATC 1 cut(s) 1562
BseAI TCCGGA 1 cut(s) 167
BseBI CCWGG 2 cut(s) 365, 944
BseDI CCNNGG 2 cut(s) 223, 1071
BseGI GGATG 4 cut(s) 10, 1024, 1229, 1351
BseJI GATNNNNATC 1 cut(s) 1562
BseLI CCNNNNNNNGG 6 cut(s) 364, 370, 524, 1093, 1492, 1759
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 2 cut(s) 1628, 1820
BseNI ACTGG 5 cut(s) 491, 1260, 1423, 1480, 1743
BseRI GAGGAG 4 cut(s) 186, 1279, 1427, 1537
BsgI GTGCAG 1 cut(s) 717
Bsh1236I CGCG 3 cut(s) 544, 717, 1460
BshFI GGCC 4 cut(s) 222, 228, 374, 411
BshNI GGYRCC 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 448
BsiSI CCGG 4 cut(s) 114, 168, 219, 1094
BslFI GGGAC 2 cut(s) 319, 1298
BslI CCNNNNNNNGG 6 cut(s) 364, 370, 524, 1093, 1492, 1759
BsmAI GTCTC 6 cut(s) 29, 138, 206, 1361, 1673, 1771
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 319, 1298
BsmI GAATGC 1 cut(s) 877
BsnI GGCC 4 cut(s) 222, 228, 374, 411
Bso31I GGTCTC 2 cut(s) 206, 1771
Bsp119I TTCGAA 2 cut(s) 126, 688
Bsp1286I GDGCHC 1 cut(s) 448
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
Bsp68I TCGCGA 1 cut(s) 544
BspACI CCGC 6 cut(s) 120, 216, 682, 1101, 1321, 1460
BspANI GGCC 4 cut(s) 222, 228, 374, 411
BspCNI CTCAG 2 cut(s) 1627, 1821
BspEI TCCGGA 1 cut(s) 167
BspFNI CGCG 3 cut(s) 544, 717, 1460
BspLI GGNNCC 1 cut(s) 118
BspMI ACCTGC 3 cut(s) 1114, 1595, 1828
BspPI GGATC 3 cut(s) 465, 520, 954
BspQI GCTCTTC 1 cut(s) 332
BspT104I TTCGAA 2 cut(s) 126, 688
BspT107I GGYRCC 1 cut(s) 116
BspTNI GGTCTC 2 cut(s) 206, 1771
BsrDI GCAATG 1 cut(s) 518
BsrFI RCCGGY 2 cut(s) 218, 1093
BsrI ACTGG 5 cut(s) 491, 1260, 1423, 1480, 1743
BssAI RCCGGY 2 cut(s) 218, 1093
BssECI CCNNGG 2 cut(s) 223, 1071
BssMI GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
BssNI GRCGYC 2 cut(s) 147, 1269
BssT1I CCWWGG 1 cut(s) 1071
Bst2UI CCWGG 2 cut(s) 365, 944
Bst4CI ACNGT 5 cut(s) 21, 40, 990, 1068, 1487
Bst6I CTCTTC 6 cut(s) 285, 332, 484, 717, 783, 1510
BstACI GRCGYC 2 cut(s) 147, 1269
BstAPI GCANNNNNTGC 1 cut(s) 1052
BstBAI YACGTR 1 cut(s) 586
BstBI TTCGAA 2 cut(s) 126, 688
BstC8I GCNNGC 7 cut(s) 220, 309, 546, 765, 915, 1332, 1401
BstDEI CTNAG 3 cut(s) 1614, 1829, 1871
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 4 cut(s) 10, 1024, 1229, 1351
BstFNI CGCG 3 cut(s) 544, 717, 1460
BstHHI GCGC 2 cut(s) 717, 1510
BstKTI GATC 7 cut(s) 405, 473, 515, 949, 1573, 1828, 1835
BstMAI GTCTC 6 cut(s) 29, 138, 206, 1361, 1673, 1771
BstMBI GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
BstMWI GCNNNNNNNGC 7 cut(s) 313, 322, 681, 1021, 1052, 1106, 1172
BstNI CCWGG 2 cut(s) 365, 944
BstNSI RCATGY 3 cut(s) 311, 811, 1330
BstSCI CCNGG 3 cut(s) 112, 363, 942
BstSFI CTRYAG 2 cut(s) 417, 1468
BstUI CGCG 3 cut(s) 544, 717, 1460
BstV2I GAAGAC 1 cut(s) 1004
BstX2I RGATCY 1 cut(s) 1825
BstYI RGATCY 1 cut(s) 1825
BsuI GTATCC 1 cut(s) 950
BsuRI GGCC 4 cut(s) 222, 228, 374, 411
BtgI CCRYGG 1 cut(s) 223
BtgZI GCGATG 2 cut(s) 759, 1441
BtrI CACGTC 1 cut(s) 1849
BtsCI GGATG 4 cut(s) 10, 1024, 1229, 1351
BtsI GCAGTG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 37
BtuMI TCGCGA 1 cut(s) 544
BveI ACCTGC 3 cut(s) 1114, 1595, 1828
Cac8I GCNNGC 7 cut(s) 220, 309, 546, 765, 915, 1332, 1401
CaiI CAGNNNCTG 2 cut(s) 881, 994
CfoI GCGC 2 cut(s) 717, 1510
Cfr10I RCCGGY 2 cut(s) 218, 1093
Cfr13I GGNCC 2 cut(s) 373, 1750
CseI GACGC 2 cut(s) 756, 1277
CsiI ACCWGGT 1 cut(s) 363
Csp6I GTAC 8 cut(s) 598, 653, 1146, 1192, 1222, 1261, 1465, 1841
CviQI GTAC 8 cut(s) 598, 653, 1146, 1192, 1222, 1261, 1465, 1841
DdeI CTNAG 3 cut(s) 1614, 1829, 1871
DpnI GATC 7 cut(s) 404, 472, 514, 948, 1572, 1827, 1834
DpnII GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
DraIII CACNNNGTG 1 cut(s) 443
DriI GACNNNNNGTC 1 cut(s) 367
EaeI YGGCCR 1 cut(s) 220
Eam1104I CTCTTC 6 cut(s) 285, 332, 484, 717, 783, 1510
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 6 cut(s) 285, 332, 484, 717, 783, 1510
Eco130I CCWWGG 1 cut(s) 1071
Eco31I GGTCTC 2 cut(s) 206, 1771
Eco47I GGWCC 1 cut(s) 1750
Eco57I CTGAAG 3 cut(s) 30, 939, 1016
EcoRII CCWGG 2 cut(s) 363, 942
EcoT14I CCWWGG 1 cut(s) 1071
EcoT22I ATGCAT 1 cut(s) 900
ErhI CCWWGG 1 cut(s) 1071
Esp3I CGTCTC 1 cut(s) 138
FalI AAGNNNNNCTT 2 cut(s) 989, 1021
FaqI GGGAC 2 cut(s) 319, 1298
FbaI TGATCA 1 cut(s) 402
FokI GGATG 4 cut(s) 17, 1031, 1216, 1358
FspBI CTAG 2 cut(s) 1650, 1897
GlaI GCGC 2 cut(s) 716, 1509
HaeIII GGCC 4 cut(s) 222, 228, 374, 411
HapII CCGG 4 cut(s) 114, 168, 219, 1094
HgaI GACGC 2 cut(s) 756, 1277
HhaI GCGC 2 cut(s) 717, 1510
Hin1I GRCGYC 2 cut(s) 147, 1269
Hin6I GCGC 2 cut(s) 715, 1508
HinP1I GCGC 2 cut(s) 715, 1508
HincII GTYRAC 2 cut(s) 973, 1312
HindII GTYRAC 2 cut(s) 973, 1312
HindIII AAGCTT 1 cut(s) 12
HpaII CCGG 4 cut(s) 114, 168, 219, 1094
HphI GGTGA 5 cut(s) 9, 507, 671, 1259, 1363
Hpy166II GTNNAC 3 cut(s) 973, 1194, 1312
Hpy188I TCNGA 9 cut(s) 143, 152, 194, 470, 512, 557, 996, 1575, 1830
Hpy188III TCNNGA 6 cut(s) 168, 543, 1028, 1385, 1616, 1753
Hpy8I GTNNAC 3 cut(s) 973, 1194, 1312
Hpy99I CGWCG 3 cut(s) 152, 722, 1445
HpyAV CCTTC 6 cut(s) 497, 517, 776, 1105, 1282, 1546
HpyCH4III ACNGT 5 cut(s) 21, 40, 990, 1068, 1487
HpyCH4IV ACGT 6 cut(s) 147, 353, 585, 1144, 1776, 1848
HpyF10VI GCNNNNNNNGC 7 cut(s) 313, 322, 681, 1021, 1052, 1106, 1172
HpyF3I CTNAG 3 cut(s) 1614, 1829, 1871
HpySE526I ACGT 6 cut(s) 147, 353, 585, 1144, 1776, 1848
Hsp92I GRCGYC 2 cut(s) 147, 1269
HspAI GCGC 2 cut(s) 715, 1508
Kpn2I TCCGGA 1 cut(s) 167
KroI GCCGGC 1 cut(s) 218
KroNI GCCGGC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 402
Kzo9I GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
LguI GCTCTTC 1 cut(s) 332
LmnI GCTCC 4 cut(s) 350, 631, 828, 854
LweI GCATC 4 cut(s) 65, 885, 1238, 1572
MabI ACCWGGT 1 cut(s) 363
MaeI CTAG 2 cut(s) 1650, 1897
MaeII ACGT 6 cut(s) 147, 353, 585, 1144, 1776, 1848
MaeIII GTNAC 1 cut(s) 1301
MalI GATC 7 cut(s) 404, 472, 514, 948, 1572, 1827, 1834
MboI GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
MboII GAAGA 9 cut(s) 188, 302, 319, 471, 734, 800, 975, 1009, 1527
MfeI CAATTG 1 cut(s) 630
MflI RGATCY 1 cut(s) 1825
MhlI GDGCHC 1 cut(s) 448
MluCI AATT 8 cut(s) 196, 574, 630, 1159, 1168, 1666, 1724, 1860
MlyI GAGTC 6 cut(s) 55, 86, 887, 1250, 1280, 1606
MmeI TCCRAC 3 cut(s) 130, 189, 270
Mph1103I ATGCAT 1 cut(s) 900
MroI TCCGGA 1 cut(s) 167
MroNI GCCGGC 1 cut(s) 218
MseI TTAA 1 cut(s) 279
MspA1I CMGCKG 1 cut(s) 917
MspI CCGG 4 cut(s) 114, 168, 219, 1094
MspR9I CCNGG 3 cut(s) 114, 365, 944
MunI CAATTG 1 cut(s) 630
Mva1269I GAATGC 1 cut(s) 877
MvaI CCWGG 2 cut(s) 365, 944
MvnI CGCG 3 cut(s) 544, 717, 1460
MwoI GCNNNNNNNGC 7 cut(s) 313, 322, 681, 1021, 1052, 1106, 1172
NaeI GCCGGC 1 cut(s) 220
NciI CCSGG 1 cut(s) 114
NdeII GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
NgoMIV GCCGGC 1 cut(s) 218
NlaIV GGNNCC 1 cut(s) 118
NmeAIII GCCGAG 1 cut(s) 504
NruI TCGCGA 1 cut(s) 544
NsiI ATGCAT 1 cut(s) 900
NspI RCATGY 3 cut(s) 311, 811, 1330
NspV TTCGAA 2 cut(s) 126, 688
PaeI GCATGC 1 cut(s) 311
PaqCI CACCTGC 1 cut(s) 1114
PciSI GCTCTTC 1 cut(s) 332
PctI GAATGC 1 cut(s) 877
PdiI GCCGGC 1 cut(s) 220
PfeI GAWTC 4 cut(s) 128, 635, 1031, 1619
PfoI TCCNGGA 1 cut(s) 942
PleI GAGTC 6 cut(s) 55, 85, 886, 1249, 1280, 1606
PpsI GAGTC 6 cut(s) 55, 85, 886, 1249, 1280, 1606
Ppu21I YACGTR 1 cut(s) 586
PshBI ATTAAT 1 cut(s) 279
Psp1406I AACGTT 1 cut(s) 353
Psp6I CCWGG 2 cut(s) 363, 942
PspGI CCWGG 2 cut(s) 363, 942
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 2 cut(s) 373, 1750
PsrI GAACNNNNNNTAC 2 cut(s) 1781, 1813
PstNI CAGNNNCTG 2 cut(s) 881, 994
PsuI RGATCY 1 cut(s) 1825
PvuII CAGCTG 1 cut(s) 917
RruI TCGCGA 1 cut(s) 544
RsaI GTAC 8 cut(s) 599, 654, 1147, 1193, 1223, 1262, 1466, 1842
RsaNI GTAC 8 cut(s) 598, 653, 1146, 1192, 1222, 1261, 1465, 1841
SapI GCTCTTC 1 cut(s) 332
SaqAI TTAA 1 cut(s) 279
Sau3AI GATC 7 cut(s) 402, 470, 512, 946, 1570, 1825, 1832
Sau96I GGNCC 2 cut(s) 373, 1750
ScaI AGTACT 2 cut(s) 654, 1466
SchI GAGTC 6 cut(s) 55, 86, 887, 1250, 1280, 1606
ScrFI CCNGG 3 cut(s) 114, 365, 944
SduI GDGCHC 1 cut(s) 448
SexAI ACCWGGT 1 cut(s) 363
SfaNI GCATC 4 cut(s) 65, 885, 1238, 1572
SfcI CTRYAG 2 cut(s) 417, 1468
SfuI TTCGAA 2 cut(s) 126, 688
SinI GGWCC 1 cut(s) 1750
SmlI CTYRAG 3 cut(s) 320, 648, 1792
SmoI CTYRAG 3 cut(s) 320, 648, 1792
SphI GCATGC 1 cut(s) 311
Sse9I AATT 8 cut(s) 196, 574, 630, 1159, 1168, 1666, 1724, 1860
SsiI CCGC 6 cut(s) 120, 216, 682, 1101, 1321, 1460
SspI AATATT 1 cut(s) 274
SspMI CTAG 2 cut(s) 1650, 1897
StyD4I CCNGG 3 cut(s) 112, 363, 942
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 5 cut(s) 21, 40, 990, 1068, 1487
TaiI ACGT 6 cut(s) 150, 356, 588, 1147, 1779, 1851
TaqI TCGA 6 cut(s) 59, 126, 688, 1215, 1443, 1539
TasI AATT 8 cut(s) 196, 574, 630, 1159, 1168, 1666, 1724, 1860
TatI WGTACW 3 cut(s) 652, 1191, 1464
TauI GCSGC 1 cut(s) 1103
TfiI GAWTC 4 cut(s) 128, 635, 1031, 1619
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 1 cut(s) 37
TspDTI ATGAA 4 cut(s) 692, 997, 1331, 1817
TspGWI ACGGA 1 cut(s) 975
TspRI CASTG 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 1750
VspI ATTAAT 1 cut(s) 279
XapI RAATTY 3 cut(s) 196, 1724, 1860
XceI RCATGY 3 cut(s) 311, 811, 1330
XspI CTAG 2 cut(s) 1650, 1897
ZraI GACGTC 1 cut(s) 148
ZrmI AGTACT 2 cut(s) 654, 1466
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.