Rh2DG322000

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011011
Physical Location & Seq
Reverse (-)
40242910 .. 40249166
6257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG322000.1

Sequence Viewer

Length: 1956 bp
ATGGAGGCCGAAGCTTCACCGTTTGAGAGCAGTGAGTTGGTGGCGAGTTTCCTGGCGTCGACGCCTTTGCTAAGCGAGTCATGGAGGCTGTGCAGCGTCGCCAACGCCGCCGCTCCATTGGGTTTCGTATCGGAGCGAATCGGAGATGTCGGGTACTTTGCTTTCTCAGCCTGCAGTACCCAAATGATCACGGAGGGGTCGGAATACTACTCCACTTGCAGAAACTTGGTGCCATTGGATAGCGCCGGCGCCGCCGCCGGTGACCGGCCTTTTTCGGTATTGACCAAAAGTAGTAACAGTAGTATCAATGGAGAAGAAGATGAGGGTGCAGTGATGGTTCATGCAGGGCTGCTCAATCTCTTCTTGTTTTTTCGTAGCTCCCAAGCTTTCAAAGACCAGATGTTGGCGCTGATAAATGGAAGCAAGTCCATTGTGATCACAGGCCATTCTATAGGAGGAACAACTGCCTCACTCTGCGCTCTTTGGCTCCTTTGTCACCTGCAATCCGTCTCTTCTACCCTTTCGGTGCTTTGCATTACCTTCGGTTCACCATTACTTGGGAACGAATCGCTTTCCCGAGCAATCCTTAGGGAAAGATGGGGTGGCAATTTTTGCCATGTGGTGTCCAAGTATGACATCATGCCAAGACTCCTCTTCGCTCCTTTAGAGTCCTGCACTCGCCAACTTCGTTTCTTGCTGCAATACTGGCAGATGTCTATGTCAGCTTCTTCTCCAGGCAACTTTGGACAGTACTTTCCAGTACTTCAACTTGGTGAGGAAGAGAAAGCTCACCTCTTCAGCTTCATATCACATTATAATTTGCTAGTGTCTTCATCAGTAGCACAAGCAGAAGCAAGAGAAGAGAAAGCAAATAGTTTGTATTGGCCATTTGGGAACTACATGTTTTGCTCACAAGAGGGAGCTATTTGTGTAGAGAATGCAGCATCTGTGATCGAGATGATGCATTTGATGTTTATGACTGGTAATCCCAGTTGCTGTATCGATGATCATCTAAAATACGGACACTATGTTGGGAAGATTTCTTCACAGTTTCTGAATAAGAAAAGCTTCTTGCAAGGGGAAGTGATTCCCGAGTCAAGCTATGAAGCAGGGGTTGCACTGGCATTGCAGTCATCAGGAATAAGTAAACAGGAACCGGCTGCCAGACCGGCGGAAGATTGCCTAAAGAAGGCTAGGGGAATGAGACATATGCGTACACCAAGCCTCAACGCTGCAAACTTAGCCATCAGATTATCCAAGGTTGTACCTTACAGAGCTCAAATAGAGTGGTATATGGCATCCTGTGATAAGTCTGATGAGCAACTAGGTTACTACGACTCCTTCAAGCAACGGGGATCATCCAAAAGGGACCACAAAGTCAACATGAACCGGCTCTTGCTTGCTGGATTTTGGAATGATGTGCTCCGCATGCTGGAGAACAACGAGCTGCCTCATGATTTTCACAAGCGATCAAAATGGGTAAACACTTCCCACTTTTACAAGCTCCTGGTAGAGCCACTGGACATTGCAGAGTATTATAGATCTGGTATGCACCGGATCAAGGGTCATTACTTGAAGCATGGAAGAGAGAGGAGGTTTGAAATTTTCGACAGGTGGTGGGGACAGAGAGAGGTTGGTCCAGAAGAAAACACTAGTATGAGAAGCAAATTTGCAGGTTTGACTCAGGACTCTTGCTTCTGGGCTAACGTGGAGGAAGCTACAGAGTGGCTAGCTAATGTTAGAAGCGAGAGTGATGCAAGGAAGCTGGCTCTGCTGTGGGAAAATATCAATAAATTTGAAAGGTATGCAACCAGTTTAGTTGATTGCAAGGAGGTCTCTAAAGACGTTGTAGCAAAGAATTCGAGCTATTCTAAGTGGGTAGAAGAGTTGAGAGAATTGAAATCTCAGATGCAGCAAATCCATCCTCAGTTCCCAAGGTTTAGAGATGGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

651

Amino Acids

73.36

Weight (kDa)

6.67

Isoelectric Point (pI)

56.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 105 - 219 2.2e-17 Lipase (class 3)
EDS1_EP PF18117 426 - 636 1.1e-71 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52430
fragaria_vesca FvH4_1g24940 FvH4_1g24940 FvH4_1g24940
malus_domestica MD15G1136300.v1.1
prunus_persica Prupe.4G276500_v2.0.a1 Prupe.4G276500_v2.0.a1
pyrus_communis pycom15g12290
rosa_chinensis RchiOBHm_Chr2g0122591
rosa_laevigata RLG00000018693
rosa_multiflora Rmu_sc0003133.1_g000001 Rmu_sc0010907.1_g000001
rosa_roxburghii Rroxscaffold_2G00121450
rosa_rugosa Rorug02G0239700 Rorug02G0239800
rosa_samantha Rh2AG298400 Rh2BG307100 Rh2CG285200 Rh2DG322000
rosa_wichuraiana Rw2G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 816
AarI CACCTGC 1 cut(s) 507
AasI GACNNNNNNGTC 1 cut(s) 1376
Acc36I ACCTGC 2 cut(s) 507, 1664
AccB1I GGYRCC 2 cut(s) 229, 248
AccB7I CCANNNNNTGG 2 cut(s) 403, 557
AccBSI CCGCTC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 59
AciI CCGC 6 cut(s) 108, 111, 252, 255, 1172, 1426
AclWI GGATC 2 cut(s) 1363, 1565
AcoI YGGCCR 1 cut(s) 884
AcsI RAATTY 4 cut(s) 1602, 1667, 1793, 1858
AcuI CTGAAG 1 cut(s) 781
AcyI GRCGYC 3 cut(s) 56, 62, 249
AfaI GTAC 6 cut(s) 155, 178, 752, 762, 1216, 1266
AfiI CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1189, 1432, 1561
AflIII ACRYGT 1 cut(s) 900
AgsI TTSAA 7 cut(s) 391, 767, 1345, 1576, 1601, 1799, 1900
AhlI ACTAGT 1 cut(s) 1652
AjnI CCWGG 3 cut(s) 51, 733, 1506
Alw21I GWGCWC 2 cut(s) 1279, 1425
Alw26I GTCTC 3 cut(s) 514, 1198, 1840
AlwI GGATC 2 cut(s) 1363, 1565
AlwNI CAGNNNCTG 3 cut(s) 947, 996, 1054
Ama87I CYCGRG 2 cut(s) 576, 1091
AoxI GGCC 4 cut(s) 6, 266, 442, 884
ApeKI GCWGC 8 cut(s) 93, 349, 697, 941, 1160, 1232, 1447, 1912
ApoI RAATTY 4 cut(s) 1602, 1667, 1793, 1858
Asp700I GAANNNNTTC 2 cut(s) 1067, 1086
AspLEI GCGC 4 cut(s) 245, 251, 409, 479
AspS9I GGNCC 2 cut(s) 1369, 1637
AsuHPI GGTGA 6 cut(s) 9, 272, 488, 540, 782, 785
AsuNHI GCTAGC 1 cut(s) 1729
AvaI CYCGRG 2 cut(s) 576, 1091
AvaII GGWCC 2 cut(s) 1369, 1637
AxyI CCTNAGG 1 cut(s) 587
BaeI ACNNNNGTAYC 2 cut(s) 286, 319
BalI TGGCCA 1 cut(s) 886
BanI GGYRCC 2 cut(s) 229, 248
BanII GRGCYC 1 cut(s) 1279
BbsI GAAGAC 1 cut(s) 822
Bbv12I GWGCWC 2 cut(s) 1279, 1425
BbvI GCAGC 8 cut(s) 105, 336, 684, 953, 1147, 1219, 1434, 1924
BccI CCATC 5 cut(s) 328, 591, 1253, 1929, 1940
BcgI CGANNNNNNTGC 8 cut(s) 49, 83, 523, 557, 1736, 1770, 1842, 1876
BciT130I CCWGG 3 cut(s) 53, 735, 1508
BclI TGATCA 3 cut(s) 186, 435, 1006
BcoDI GTCTC 3 cut(s) 514, 1198, 1840
BcuI ACTAGT 1 cut(s) 1652
BfaI CTAG 5 cut(s) 824, 1194, 1325, 1653, 1730
BfmI CTRYAG 3 cut(s) 172, 450, 1719
BfoI RGCGCY 3 cut(s) 246, 252, 410
BfuAI ACCTGC 2 cut(s) 507, 1664
BglI GCCNNNNNGGC 1 cut(s) 1169
BglII AGATCT 1 cut(s) 1541
BlpI GCTNAGC 1 cut(s) 71
BmcAI AGTACT 2 cut(s) 752, 762
Bme1390I CCNGG 3 cut(s) 53, 735, 1508
Bme18I GGWCC 2 cut(s) 1369, 1637
BmeT110I CYCGRG 2 cut(s) 576, 1091
BmgT120I GGNCC 2 cut(s) 1369, 1637
BmiI GGNNCC 5 cut(s) 231, 250, 488, 1155, 1370
BmrFI CCNGG 3 cut(s) 53, 735, 1508
BmrI ACTGGG 1 cut(s) 984
BmsI GCATC 5 cut(s) 951, 953, 1307, 1744, 1899
BmtI GCTAGC 1 cut(s) 1733
BmuI ACTGGG 1 cut(s) 984
BpiI GAAGAC 1 cut(s) 822
BpmI CTGGAG 2 cut(s) 717, 1454
Bpu1102I GCTNAGC 1 cut(s) 71
Bsa29I ATCGAT 1 cut(s) 1002
BsaBI GATNNNNATC 1 cut(s) 1008
BsaHI GRCGYC 3 cut(s) 56, 62, 249
BsaI GGTCTC 1 cut(s) 1840
BsaJI CCNNGG 2 cut(s) 1257, 1934
BsaWI WCCGGW 1 cut(s) 1554
BsaXI ACNNNNNCTCC 2 cut(s) 1322, 1352
Bsc4I CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1189, 1432, 1561
Bse118I RCCGGY 6 cut(s) 245, 257, 264, 1156, 1168, 1389
Bse1I ACTGG 7 cut(s) 710, 758, 985, 990, 1125, 1524, 1812
Bse21I CCTNAGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 1124, 1524
Bse8I GATNNNNATC 1 cut(s) 1008
BseBI CCWGG 3 cut(s) 53, 735, 1508
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 2 cut(s) 1257, 1934
BseGI GGATG 3 cut(s) 1298, 1358, 1921
BseJI GATNNNNATC 1 cut(s) 1008
BseLI CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1189, 1432, 1561
BseMI GCAATG 2 cut(s) 1124, 1524
BseMII CTCAG 4 cut(s) 180, 1697, 1919, 1940
BseNI ACTGG 7 cut(s) 710, 758, 985, 990, 1125, 1524, 1812
BseRI GAGGAG 2 cut(s) 641, 1606
BseXI GCAGC 8 cut(s) 105, 336, 684, 953, 1147, 1219, 1434, 1924
BsgI GTGCAG 3 cut(s) 112, 348, 658
BshFI GGCC 4 cut(s) 8, 268, 444, 886
BshNI GGYRCC 2 cut(s) 229, 248
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 2 cut(s) 1279, 1425
BsiHKCI CYCGRG 2 cut(s) 576, 1091
BsiSI CCGG 7 cut(s) 246, 258, 265, 1157, 1169, 1390, 1555
BslFI GGGAC 2 cut(s) 1382, 1635
BslI CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1189, 1432, 1561
BsmAI GTCTC 3 cut(s) 514, 1198, 1840
BsmBI CGTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 1382, 1635
BsmI GAATGC 1 cut(s) 943
BsnI GGCC 4 cut(s) 8, 268, 444, 886
Bso31I GGTCTC 1 cut(s) 1840
BsoBI CYCGRG 2 cut(s) 576, 1091
Bsp1286I GDGCHC 2 cut(s) 1279, 1425
Bsp143I GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
Bsp1720I GCTNAGC 1 cut(s) 71
BspACI CCGC 6 cut(s) 108, 111, 252, 255, 1172, 1426
BspANI GGCC 4 cut(s) 8, 268, 444, 886
BspCNI CTCAG 4 cut(s) 179, 1696, 1918, 1939
BspDI ATCGAT 1 cut(s) 1002
BspHI TCATGA 1 cut(s) 1453
BspLI GGNNCC 5 cut(s) 231, 250, 488, 1155, 1370
BspMAI CTGCAG 1 cut(s) 176
BspMI ACCTGC 2 cut(s) 507, 1664
BspOI GCTAGC 1 cut(s) 1733
BspPI GGATC 2 cut(s) 1363, 1565
BspT107I GGYRCC 2 cut(s) 229, 248
BspTNI GGTCTC 1 cut(s) 1840
BsrBI CCGCTC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 1124, 1524
BsrFI RCCGGY 6 cut(s) 245, 257, 264, 1156, 1168, 1389
BsrI ACTGG 7 cut(s) 710, 758, 985, 990, 1125, 1524, 1812
BssAI RCCGGY 6 cut(s) 245, 257, 264, 1156, 1168, 1389
BssECI CCNNGG 2 cut(s) 1257, 1934
BssMI GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
BssNI GRCGYC 3 cut(s) 56, 62, 249
BssT1I CCWWGG 2 cut(s) 1257, 1934
Bst2UI CCWGG 3 cut(s) 53, 735, 1508
Bst4CI ACNGT 4 cut(s) 21, 299, 750, 1050
Bst6I CTCTTC 8 cut(s) 365, 517, 659, 774, 800, 855, 1579, 1878
BstACI GRCGYC 3 cut(s) 56, 62, 249
BstAPI GCANNNNNTGC 2 cut(s) 612, 1115
BstC8I GCNNGC 6 cut(s) 172, 247, 1401, 1430, 1731, 1767
BstDEI CTNAG 8 cut(s) 71, 166, 587, 1240, 1683, 1872, 1905, 1926
BstEII GGTNACC 1 cut(s) 260
BstENI CCTNNNNNAGG 1 cut(s) 1187
BstF5I GGATG 3 cut(s) 1298, 1358, 1921
BstH2I RGCGCY 3 cut(s) 246, 252, 410
BstHHI GCGC 4 cut(s) 245, 251, 409, 479
BstKTI GATC 8 cut(s) 189, 438, 954, 1009, 1358, 1472, 1544, 1560
BstMAI GTCTC 3 cut(s) 514, 1198, 1840
BstMBI GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
BstNI CCWGG 3 cut(s) 53, 735, 1508
BstNSI RCATGY 2 cut(s) 904, 1432
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 51, 733, 1506
BstSFI CTRYAG 3 cut(s) 172, 450, 1719
BstV1I GCAGC 8 cut(s) 105, 336, 684, 953, 1147, 1219, 1434, 1924
BstV2I GAAGAC 1 cut(s) 822
BstX2I RGATCY 1 cut(s) 1541
BstYI RGATCY 1 cut(s) 1541
Bsu15I ATCGAT 1 cut(s) 1002
Bsu36I CCTNAGG 1 cut(s) 587
BsuRI GGCC 4 cut(s) 8, 268, 444, 886
BsuTUI ATCGAT 1 cut(s) 1002
BtsCI GGATG 3 cut(s) 1298, 1358, 1921
BtsI GCAGTG 2 cut(s) 37, 336
BtsIMutI CAGTG 4 cut(s) 37, 336, 1118, 1517
BveI ACCTGC 2 cut(s) 507, 1664
Cac8I GCNNGC 6 cut(s) 172, 247, 1401, 1430, 1731, 1767
CaiI CAGNNNCTG 3 cut(s) 947, 996, 1054
CciI TCATGA 1 cut(s) 1453
CfoI GCGC 4 cut(s) 245, 251, 409, 479
Cfr10I RCCGGY 6 cut(s) 245, 257, 264, 1156, 1168, 1389
Cfr13I GGNCC 2 cut(s) 1369, 1637
ClaI ATCGAT 1 cut(s) 1002
CseI GACGC 3 cut(s) 45, 70, 85
Csp6I GTAC 6 cut(s) 154, 177, 751, 761, 1215, 1265
CspCI CAANNNNNGTGG 2 cut(s) 1268, 1303
CviAII CATG 9 cut(s) 81, 341, 617, 640, 901, 1384, 1429, 1454, 1580
CviQI GTAC 6 cut(s) 154, 177, 751, 761, 1215, 1265
DdeI CTNAG 8 cut(s) 71, 166, 587, 1240, 1683, 1872, 1905, 1926
DinI GGCGCC 1 cut(s) 250
DpnI GATC 8 cut(s) 188, 437, 953, 1008, 1357, 1471, 1543, 1559
DpnII GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
DrdI GACNNNNNNGTC 1 cut(s) 1376
DseDI GACNNNNNNGTC 1 cut(s) 1376
EaeI YGGCCR 1 cut(s) 884
Eam1104I CTCTTC 8 cut(s) 365, 517, 659, 774, 800, 855, 1579, 1878
EarI CTCTTC 8 cut(s) 365, 517, 659, 774, 800, 855, 1579, 1878
EciI GGCGGA 1 cut(s) 1187
Ecl136II GAGCTC 1 cut(s) 1277
Eco130I CCWWGG 2 cut(s) 1257, 1934
Eco24I GRGCYC 1 cut(s) 1279
Eco31I GGTCTC 1 cut(s) 1840
Eco47I GGWCC 2 cut(s) 1369, 1637
Eco53kI GAGCTC 1 cut(s) 1277
Eco57I CTGAAG 1 cut(s) 781
Eco81I CCTNAGG 1 cut(s) 587
Eco88I CYCGRG 2 cut(s) 576, 1091
Eco91I GGTNACC 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 1277
EcoNI CCTNNNNNAGG 1 cut(s) 1187
EcoO65I GGTNACC 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 1858
EcoRII CCWGG 3 cut(s) 51, 733, 1506
EcoT14I CCWWGG 2 cut(s) 1257, 1934
EcoT22I ATGCAT 1 cut(s) 966
EcoT38I GRGCYC 1 cut(s) 1279
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
ErhI CCWWGG 2 cut(s) 1257, 1934
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 9 cut(s) 84, 344, 620, 643, 904, 1387, 1432, 1457, 1583
FalI AAGNNNNNCTT 2 cut(s) 1052, 1084
FaqI GGGAC 2 cut(s) 1382, 1635
FatI CATG 9 cut(s) 80, 340, 616, 639, 900, 1383, 1428, 1453, 1579
FauNDI CATATG 1 cut(s) 1209
FbaI TGATCA 3 cut(s) 186, 435, 1006
FblI GTMKAC 1 cut(s) 59
FokI GGATG 3 cut(s) 1285, 1345, 1908
FriOI GRGCYC 1 cut(s) 1279
FspBI CTAG 5 cut(s) 824, 1194, 1325, 1653, 1730
GlaI GCGC 4 cut(s) 244, 250, 408, 478
GsuI CTGGAG 2 cut(s) 717, 1454
HaeII RGCGCY 3 cut(s) 246, 252, 410
HaeIII GGCC 4 cut(s) 8, 268, 444, 886
HapII CCGG 7 cut(s) 246, 258, 265, 1157, 1169, 1390, 1555
HgaI GACGC 3 cut(s) 45, 70, 85
HhaI GCGC 4 cut(s) 245, 251, 409, 479
Hin1I GRCGYC 3 cut(s) 56, 62, 249
Hin1II CATG 9 cut(s) 84, 344, 620, 643, 904, 1387, 1432, 1457, 1583
Hin6I GCGC 4 cut(s) 243, 249, 407, 477
HinP1I GCGC 4 cut(s) 243, 249, 407, 477
HincII GTYRAC 2 cut(s) 60, 1381
HindII GTYRAC 2 cut(s) 60, 1381
HindIII AAGCTT 3 cut(s) 12, 384, 1066
HpaII CCGG 7 cut(s) 246, 258, 265, 1157, 1169, 1390, 1555
HphI GGTGA 6 cut(s) 9, 272, 488, 540, 782, 785
Hpy166II GTNNAC 6 cut(s) 60, 548, 1148, 1217, 1381, 1483
Hpy188I TCNGA 7 cut(s) 133, 143, 202, 1056, 1250, 1315, 1908
Hpy188III TCNNGA 7 cut(s) 576, 955, 1091, 1137, 1454, 1640, 1685
Hpy8I GTNNAC 6 cut(s) 60, 548, 1148, 1217, 1381, 1483
Hpy99I CGWCG 3 cut(s) 61, 64, 101
HpyAV CCTTC 3 cut(s) 550, 1183, 1351
HpyCH4III ACNGT 4 cut(s) 21, 299, 750, 1050
HpyCH4IV ACGT 2 cut(s) 1707, 1845
HpyF3I CTNAG 8 cut(s) 71, 166, 587, 1240, 1683, 1872, 1905, 1926
HpySE526I ACGT 2 cut(s) 1707, 1845
Hsp92I GRCGYC 3 cut(s) 56, 62, 249
Hsp92II CATG 9 cut(s) 84, 344, 620, 643, 904, 1387, 1432, 1457, 1583
HspAI GCGC 4 cut(s) 243, 249, 407, 477
KasI GGCGCC 1 cut(s) 248
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
Ksp22I TGATCA 3 cut(s) 186, 435, 1006
Kzo9I GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
LmnI GCTCC 8 cut(s) 118, 133, 383, 492, 664, 920, 1428, 1509
Lsp1109I GCAGC 8 cut(s) 105, 336, 684, 953, 1147, 1219, 1434, 1924
LweI GCATC 5 cut(s) 951, 953, 1307, 1744, 1899
MaeI CTAG 5 cut(s) 824, 1194, 1325, 1653, 1730
MaeII ACGT 2 cut(s) 1707, 1845
MaeIII GTNAC 4 cut(s) 260, 293, 494, 1328
MalI GATC 8 cut(s) 188, 437, 953, 1008, 1357, 1471, 1543, 1559
MbiI CCGCTC 1 cut(s) 113
MboI GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
MflI RGATCY 1 cut(s) 1541
MhlI GDGCHC 2 cut(s) 1279, 1425
MlsI TGGCCA 1 cut(s) 886
MluCI AATT 7 cut(s) 607, 817, 1602, 1667, 1793, 1858, 1895
MluNI TGGCCA 1 cut(s) 886
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 7 cut(s) 86, 642, 677, 1103, 1331, 1675, 1682
MmeI TCCRAC 1 cut(s) 180
Mox20I TGGCCA 1 cut(s) 886
Mph1103I ATGCAT 1 cut(s) 966
MreI CGCCGGCG 1 cut(s) 245
MroNI GCCGGC 1 cut(s) 245
MroXI GAANNNNTTC 2 cut(s) 1067, 1086
MscI TGGCCA 1 cut(s) 886
MslI CAYNNNNRTG 1 cut(s) 1655
Msp20I TGGCCA 1 cut(s) 886
MspI CCGG 7 cut(s) 246, 258, 265, 1157, 1169, 1390, 1555
MspR9I CCNGG 3 cut(s) 53, 735, 1508
Mva1269I GAATGC 1 cut(s) 943
MvaI CCWGG 3 cut(s) 53, 735, 1508
NaeI GCCGGC 1 cut(s) 247
NarI GGCGCC 1 cut(s) 249
NdeI CATATG 1 cut(s) 1209
NdeII GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
NgoMIV GCCGGC 1 cut(s) 245
NheI GCTAGC 1 cut(s) 1729
NlaIII CATG 9 cut(s) 84, 344, 620, 643, 904, 1387, 1432, 1457, 1583
NlaIV GGNNCC 5 cut(s) 231, 250, 488, 1155, 1370
NmuCI GTSAC 2 cut(s) 260, 494
NsiI ATGCAT 1 cut(s) 966
NspI RCATGY 2 cut(s) 904, 1432
PaeI GCATGC 1 cut(s) 1432
PagI TCATGA 1 cut(s) 1453
PaqCI CACCTGC 1 cut(s) 507
PciI ACATGT 1 cut(s) 900
PcsI WCGNNNNNNNCGW 1 cut(s) 685
PctI GAATGC 1 cut(s) 943
PdiI GCCGGC 1 cut(s) 247
PdmI GAANNNNTTC 2 cut(s) 1067, 1086
PfeI GAWTC 3 cut(s) 138, 566, 1087
PflMI CCANNNNNTGG 2 cut(s) 403, 557
PleI GAGTC 7 cut(s) 85, 642, 676, 1102, 1331, 1675, 1682
PluTI GGCGCC 1 cut(s) 252
PpsI GAGTC 7 cut(s) 85, 642, 676, 1102, 1331, 1675, 1682
PscI ACATGT 1 cut(s) 900
PsiI TTATAA 1 cut(s) 816
Psp124BI GAGCTC 1 cut(s) 1279
Psp6I CCWGG 3 cut(s) 51, 733, 1506
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 3 cut(s) 51, 733, 1506
PspN4I GGNNCC 5 cut(s) 231, 250, 488, 1155, 1370
PspPI GGNCC 2 cut(s) 1369, 1637
PstI CTGCAG 1 cut(s) 176
PstNI CAGNNNCTG 3 cut(s) 947, 996, 1054
PsuI RGATCY 1 cut(s) 1541
RsaI GTAC 6 cut(s) 155, 178, 752, 762, 1216, 1266
RsaNI GTAC 6 cut(s) 154, 177, 751, 761, 1215, 1265
RseI CAYNNNNRTG 1 cut(s) 1655
SacI GAGCTC 1 cut(s) 1279
SalI GTCGAC 1 cut(s) 58
Sau3AI GATC 8 cut(s) 186, 435, 951, 1006, 1355, 1469, 1541, 1557
Sau96I GGNCC 2 cut(s) 1369, 1637
ScaI AGTACT 2 cut(s) 752, 762
SchI GAGTC 7 cut(s) 86, 642, 677, 1103, 1331, 1675, 1682
ScrFI CCNGG 3 cut(s) 53, 735, 1508
SduI GDGCHC 2 cut(s) 1279, 1425
SfaNI GCATC 5 cut(s) 951, 953, 1307, 1744, 1899
SfcI CTRYAG 3 cut(s) 172, 450, 1719
SfoI GGCGCC 1 cut(s) 250
SgrAI CRCCGGYG 2 cut(s) 245, 257
SgrDI CGTCGACG 1 cut(s) 58
SinI GGWCC 2 cut(s) 1369, 1637
SmiMI CAYNNNNRTG 1 cut(s) 1655
SpeI ACTAGT 1 cut(s) 1652
SphI GCATGC 1 cut(s) 1432
Sse9I AATT 7 cut(s) 607, 817, 1602, 1667, 1793, 1858, 1895
SsiI CCGC 6 cut(s) 108, 111, 252, 255, 1172, 1426
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 5 cut(s) 824, 1194, 1325, 1653, 1730
SstI GAGCTC 1 cut(s) 1279
StyD4I CCNGG 3 cut(s) 51, 733, 1506
StyI CCWWGG 2 cut(s) 1257, 1934
TaaI ACNGT 4 cut(s) 21, 299, 750, 1050
TaiI ACGT 2 cut(s) 1710, 1848
TaqI TCGA 5 cut(s) 59, 954, 1002, 1608, 1862
TasI AATT 7 cut(s) 607, 817, 1602, 1667, 1793, 1858, 1895
TatI WGTACW 2 cut(s) 750, 760
TauI GCSGC 4 cut(s) 110, 113, 254, 257
TfiI GAWTC 3 cut(s) 138, 566, 1087
TscAI CASTG 4 cut(s) 37, 336, 1125, 1524
TseFI GTSAC 2 cut(s) 260, 494
TseI GCWGC 8 cut(s) 93, 349, 697, 941, 1160, 1232, 1447, 1912
Tsp45I GTSAC 2 cut(s) 260, 494
TspDTI ATGAA 5 cut(s) 329, 793, 822, 1119, 1400
TspGWI ACGGA 3 cut(s) 206, 496, 1035
TspRI CASTG 4 cut(s) 37, 336, 1125, 1524
Van91I CCANNNNNTGG 2 cut(s) 403, 557
VpaK11BI GGWCC 2 cut(s) 1369, 1637
XagI CCTNNNNNAGG 1 cut(s) 1187
XapI RAATTY 4 cut(s) 1602, 1667, 1793, 1858
XceI RCATGY 2 cut(s) 904, 1432
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 2 cut(s) 1067, 1086
XspI CTAG 5 cut(s) 824, 1194, 1325, 1653, 1730
ZrmI AGTACT 2 cut(s) 752, 762
Zsp2I ATGCAT 1 cut(s) 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.