RchiOBHm_Chr2g0122591

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0011011
Physical Location & Seq
Reverse (-)
35605802 .. 35610468
4667 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49497

Sequence Viewer

Length: 1812 bp
ATGGAGGCCGAAGCTTCACCGTTTGAGAGCAGTGAGTTGGTGGCGAGTTTCCTGGCCTCGACGCCTTTGCTAAGCGAGTCATGGAGGCTGTGCAGCGTCGCCAACGCCTCCGCTCCATTGGGTTTCGTATCGGAGCGAATCGGAGATGTCGGGTACTTTGCTTTCTCGGCCTGCAATGAGGGTGCAGTGATGGTTCATGCAGGGCTGCTCAATCTCTTCTTGTTTTTTCGTAGCTCCCGAGCTTTCCAAGACCAGATGTTGGCGCTGATAAATGGAAGCAAGTCCATTGTGATCACAGGCCATTCTATAGGAGGAACAACTGCCTCACTCTGCGCTCTTTGGATCCTTTGCTACCTGCAATCCGTCTCTTCTACCCTTTCAGTGCTTTGCATTACCTTCGGTTCACCATTACTTGGGAACGCATCTCTTTCCCGAGCAATCCTTAGGGAAAGATGGGGTGGCAATTTTTGCCATGTGGTGTCCAAGTATGACATCATGCCAAGACTCCTATTCGCTCCTTTAGAGTCCTGCACTCGCCAACTCCATTTCTTGCTGCAATACTGGCAGATGTCTATGTCGGCTTCTTCTCCAGGCAACTTTGGACAGTCTCCAGTACTTCAACTCGGTGAGGAAGAGAAAGCTCAACTCTTCAGCTTCATATCACATTCTAATTTGCTAGTGTCTTCAGTAGCACAAGCAGAAGCAAGAGAAGAGAAAGCAAATAGTTTGTATTGGCCATTTGGAAACTACGTGTTTTGCTCACAAGAGGGAGCTATTTGTGTGGAGAATGCAGCATCTGTGATCGAGATGATGCATTTGATGTTTATGACTGGTAATCCCAGTTGCTGTATCGATGATCATCTAAAATACGGACACTATGTTGGGAAGATTTCTTCACAGTTTCTGAGTAAGAAAAGCTTCTTGCAAGGGGAAGTGATTCCTGAGTCAAGCTATGAAGCAGGGGTTGAACTGGCATTGCAGTCATCAGGAATAAGTAAACAGGAACCAGCTGCCAGACCGGCGGAAGATTGCCTAAAGAAGGCTAGGGGAATGAGGCATATGCGTACACCAAGCCTCAACGCTGCAAACTTAGCCATCAGATTATCCAAGGTTGTACCTTACAGAGCTCAAATAGAGTGGTATATGGCATCCTGTGATAAGTCTGATGAGCAACTAGGTTACTACGACTCCTTCAAGCAACGGGGATCATCCAAAAGGGACCACAAAGTCAACATGAACCGGCTCTTGCTTGCTGGATTTTGGAATGATGTGCTCCGCATGCTGGAGAACAACGAGCTGCCTCATGATTTTCACAAGCGATCAAAATGGGTAAACACTTCCCACTCTTACAAGCTCCTGGTAGAGCCACTGGACATTGCTGAGTACTATAGATCTGGTACGCACCGGATCAAGGGTCATTACTTGAAGCATGGAAGAGAGAGGAGGTTTGAAATTTTCGACAGGTGGTGGGGACAGAGAGAGGTTGGTCCAGAAGAAAACACTAGTATGAGAAGCAAATTTGCAGGTTTGACTCAGGACTCTTGCTTCTGGGCTAAGGTGGAGGAAGCTACAGAGTGGCTAGCTAATGTTAGAAGCGAGAGTGATGCAAGGAAGCTGGCTCTGCTGTGGGAAAATATCAATAACTTTGAAAGGTATGCAACCAGATTAGTTGATTGCAAGGAAGTCTCTAAAGACGTTGTAGCAAAGAATTCGAGCTATTCTAAGTGGGTAGAAGAGTTGAGAGAATTGAAATCTCAGATGCAGCAAATCCATCCTCAGTTCCCAAGGTTTAATGGTGAGTTATTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

603

Amino Acids

68.17

Weight (kDa)

7.24

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 59 - 171 3.3e-16 Lipase (class 3)
EDS1_EP PF18117 376 - 586 3.3e-71 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52430
fragaria_vesca FvH4_1g24940 FvH4_1g24940 FvH4_1g24940
malus_domestica MD15G1136300.v1.1
prunus_persica Prupe.4G276500_v2.0.a1 Prupe.4G276500_v2.0.a1
pyrus_communis pycom15g12290
rosa_chinensis RchiOBHm_Chr2g0122591
rosa_laevigata RLG00000018693
rosa_multiflora Rmu_sc0003133.1_g000001 Rmu_sc0010907.1_g000001
rosa_roxburghii Rroxscaffold_2G00121450
rosa_rugosa Rorug02G0239700 Rorug02G0239800
rosa_samantha Rh2AG298400 Rh2BG307100 Rh2CG285200 Rh2DG322000
rosa_wichuraiana Rw2G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1226
Acc36I ACCTGC 2 cut(s) 363, 1514
AccB7I CCANNNNNTGG 2 cut(s) 259, 413
AccBSI CCGCTC 1 cut(s) 113
AciI CCGC 3 cut(s) 111, 1022, 1276
AclWI GGATC 4 cut(s) 337, 350, 1213, 1415
AcoI YGGCCR 1 cut(s) 734
AcsI RAATTY 3 cut(s) 1452, 1517, 1708
AcuI CTGAAG 2 cut(s) 634, 669
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 6 cut(s) 155, 615, 1066, 1116, 1385, 1399
AfiI CCNNNNNNNGG 5 cut(s) 259, 413, 1039, 1282, 1411
AflIII ACRYGT 1 cut(s) 750
AgsI TTSAA 7 cut(s) 620, 968, 1195, 1426, 1451, 1649, 1750
AhlI ACTAGT 1 cut(s) 1502
AjnI CCWGG 3 cut(s) 51, 589, 1356
Alw21I GWGCWC 2 cut(s) 1129, 1275
Alw26I GTCTC 3 cut(s) 370, 612, 1690
AlwI GGATC 4 cut(s) 337, 350, 1213, 1415
AlwNI CAGNNNCTG 3 cut(s) 797, 846, 904
Ama87I CYCGRG 2 cut(s) 237, 432
AoxI GGCC 5 cut(s) 6, 54, 168, 298, 734
ApeKI GCWGC 8 cut(s) 93, 205, 553, 791, 1010, 1082, 1297, 1762
ApoI RAATTY 3 cut(s) 1452, 1517, 1708
Asp700I GAANNNNTTC 2 cut(s) 917, 936
AspLEI GCGC 2 cut(s) 265, 335
AspS9I GGNCC 2 cut(s) 1219, 1487
AsuHPI GGTGA 4 cut(s) 9, 396, 638, 1808
AsuNHI GCTAGC 1 cut(s) 1579
AvaI CYCGRG 2 cut(s) 237, 432
AvaII GGWCC 2 cut(s) 1219, 1487
AxyI CCTNAGG 1 cut(s) 443
BalI TGGCCA 1 cut(s) 736
BamHI GGATCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 1129
BbsI GAAGAC 1 cut(s) 675
Bbv12I GWGCWC 2 cut(s) 1129, 1275
BbvI GCAGC 8 cut(s) 105, 192, 540, 803, 997, 1069, 1284, 1774
BccI CCATC 4 cut(s) 184, 447, 1103, 1779
BcgI CGANNNNNNTGC 8 cut(s) 49, 83, 379, 413, 1586, 1620, 1692, 1726
BciT130I CCWGG 3 cut(s) 53, 591, 1358
BclI TGATCA 2 cut(s) 291, 856
BcoDI GTCTC 3 cut(s) 370, 612, 1690
BcuI ACTAGT 1 cut(s) 1502
BfaI CTAG 5 cut(s) 677, 1044, 1175, 1503, 1580
BfmI CTRYAG 3 cut(s) 306, 1387, 1569
BfoI RGCGCY 1 cut(s) 266
BfuAI ACCTGC 2 cut(s) 363, 1514
BglI GCCNNNNNGGC 1 cut(s) 1019
BglII AGATCT 1 cut(s) 1391
BisI GCNGC 8 cut(s) 94, 206, 554, 792, 1011, 1083, 1298, 1763
BlpI GCTNAGC 1 cut(s) 71
BlsI GCNGC 8 cut(s) 95, 207, 555, 793, 1012, 1084, 1299, 1764
BmcAI AGTACT 2 cut(s) 615, 1385
Bme1390I CCNGG 3 cut(s) 53, 591, 1358
Bme18I GGWCC 2 cut(s) 1219, 1487
BmeT110I CYCGRG 2 cut(s) 237, 432
BmgT120I GGNCC 2 cut(s) 1219, 1487
BmiI GGNNCC 3 cut(s) 344, 1005, 1220
BmrFI CCNGG 3 cut(s) 53, 591, 1358
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 6 cut(s) 431, 801, 803, 1157, 1594, 1749
BmtI GCTAGC 1 cut(s) 1583
BmuI ACTGGG 1 cut(s) 834
BpiI GAAGAC 1 cut(s) 675
BpmI CTGGAG 3 cut(s) 573, 594, 1304
Bpu10I CCTNAGC 1 cut(s) 1554
Bpu1102I GCTNAGC 1 cut(s) 71
Bsa29I ATCGAT 1 cut(s) 852
BsaAI YACGTR 1 cut(s) 751
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 1107, 1784
BsaWI WCCGGW 1 cut(s) 1404
BsaXI ACNNNNNCTCC 2 cut(s) 1172, 1202
Bsc4I CCNNNNNNNGG 5 cut(s) 259, 413, 1039, 1282, 1411
Bse118I RCCGGY 2 cut(s) 1018, 1239
Bse1I ACTGG 6 cut(s) 566, 611, 835, 840, 975, 1374
Bse21I CCTNAGG 1 cut(s) 443
Bse3DI GCAATG 3 cut(s) 181, 974, 1374
Bse8I GATNNNNATC 1 cut(s) 858
BseBI CCWGG 3 cut(s) 53, 591, 1358
BseCI ATCGAT 1 cut(s) 852
BseDI CCNNGG 2 cut(s) 1107, 1784
BseGI GGATG 3 cut(s) 1148, 1208, 1771
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 5 cut(s) 259, 413, 1039, 1282, 1411
BseMI GCAATG 3 cut(s) 181, 974, 1374
BseMII CTCAG 6 cut(s) 896, 933, 1371, 1547, 1769, 1790
BseNI ACTGG 6 cut(s) 566, 611, 835, 840, 975, 1374
BseRI GAGGAG 1 cut(s) 1456
BseXI GCAGC 8 cut(s) 105, 192, 540, 803, 997, 1069, 1284, 1774
BsgI GTGCAG 3 cut(s) 112, 204, 514
BshFI GGCC 5 cut(s) 8, 56, 170, 300, 736
BshVI ATCGAT 1 cut(s) 852
BsiHKAI GWGCWC 2 cut(s) 1129, 1275
BsiHKCI CYCGRG 2 cut(s) 237, 432
BsiSI CCGG 3 cut(s) 1019, 1240, 1405
BslFI GGGAC 2 cut(s) 1232, 1485
BslI CCNNNNNNNGG 5 cut(s) 259, 413, 1039, 1282, 1411
BsmAI GTCTC 3 cut(s) 370, 612, 1690
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 2 cut(s) 1232, 1485
BsmI GAATGC 1 cut(s) 793
BsnI GGCC 5 cut(s) 8, 56, 170, 300, 736
BsoBI CYCGRG 2 cut(s) 237, 432
Bsp1286I GDGCHC 2 cut(s) 1129, 1275
Bsp143I GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
Bsp1720I GCTNAGC 1 cut(s) 71
BspACI CCGC 3 cut(s) 111, 1022, 1276
BspANI GGCC 5 cut(s) 8, 56, 170, 300, 736
BspCNI CTCAG 6 cut(s) 897, 934, 1372, 1546, 1768, 1789
BspDI ATCGAT 1 cut(s) 852
BspHI TCATGA 1 cut(s) 1303
BspLI GGNNCC 3 cut(s) 344, 1005, 1220
BspMI ACCTGC 2 cut(s) 363, 1514
BspOI GCTAGC 1 cut(s) 1583
BspPI GGATC 4 cut(s) 337, 350, 1213, 1415
BsrBI CCGCTC 1 cut(s) 113
BsrDI GCAATG 3 cut(s) 181, 974, 1374
BsrFI RCCGGY 2 cut(s) 1018, 1239
BsrI ACTGG 6 cut(s) 566, 611, 835, 840, 975, 1374
BssAI RCCGGY 2 cut(s) 1018, 1239
BssECI CCNNGG 2 cut(s) 1107, 1784
BssMI GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 2 cut(s) 1107, 1784
Bst2UI CCWGG 3 cut(s) 53, 591, 1358
Bst4CI ACNGT 3 cut(s) 21, 606, 900
Bst6I CTCTTC 7 cut(s) 221, 373, 627, 653, 705, 1429, 1728
BstACI GRCGYC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 468
BstBAI YACGTR 1 cut(s) 751
BstC8I GCNNGC 5 cut(s) 172, 1251, 1280, 1581, 1617
BstENI CCTNNNNNAGG 1 cut(s) 1037
BstF5I GGATG 3 cut(s) 1148, 1208, 1771
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 2 cut(s) 265, 335
BstKTI GATC 8 cut(s) 294, 345, 804, 859, 1208, 1322, 1394, 1410
BstMAI GTCTC 3 cut(s) 370, 612, 1690
BstMBI GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
BstMWI GCNNNNNNNGC 7 cut(s) 167, 468, 562, 1019, 1091, 1279, 1621
BstNI CCWGG 3 cut(s) 53, 591, 1358
BstNSI RCATGY 1 cut(s) 1282
BstSCI CCNGG 3 cut(s) 51, 589, 1356
BstSFI CTRYAG 3 cut(s) 306, 1387, 1569
BstV1I GCAGC 8 cut(s) 105, 192, 540, 803, 997, 1069, 1284, 1774
BstV2I GAAGAC 1 cut(s) 675
BstX2I RGATCY 2 cut(s) 342, 1391
BstYI RGATCY 2 cut(s) 342, 1391
Bsu15I ATCGAT 1 cut(s) 852
Bsu36I CCTNAGG 1 cut(s) 443
BsuRI GGCC 5 cut(s) 8, 56, 170, 300, 736
BsuTUI ATCGAT 1 cut(s) 852
BtsCI GGATG 3 cut(s) 1148, 1208, 1771
BtsI GCAGTG 2 cut(s) 37, 192
BtsIMutI CAGTG 4 cut(s) 37, 192, 387, 1367
BveI ACCTGC 2 cut(s) 363, 1514
Cac8I GCNNGC 5 cut(s) 172, 1251, 1280, 1581, 1617
CaiI CAGNNNCTG 3 cut(s) 797, 846, 904
CciI TCATGA 1 cut(s) 1303
CfoI GCGC 2 cut(s) 265, 335
Cfr10I RCCGGY 2 cut(s) 1018, 1239
Cfr13I GGNCC 2 cut(s) 1219, 1487
ClaI ATCGAT 1 cut(s) 852
CseI GACGC 2 cut(s) 70, 85
Csp6I GTAC 6 cut(s) 154, 614, 1065, 1115, 1384, 1398
CspCI CAANNNNNGTGG 2 cut(s) 1118, 1153
CviAII CATG 8 cut(s) 81, 197, 473, 496, 1234, 1279, 1304, 1430
CviQI GTAC 6 cut(s) 154, 614, 1065, 1115, 1384, 1398
DpnI GATC 8 cut(s) 293, 344, 803, 858, 1207, 1321, 1393, 1409
DpnII GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
DrdI GACNNNNNNGTC 1 cut(s) 1226
DseDI GACNNNNNNGTC 1 cut(s) 1226
EaeI YGGCCR 1 cut(s) 734
Eam1104I CTCTTC 7 cut(s) 221, 373, 627, 653, 705, 1429, 1728
EarI CTCTTC 7 cut(s) 221, 373, 627, 653, 705, 1429, 1728
EciI GGCGGA 1 cut(s) 1037
Ecl136II GAGCTC 1 cut(s) 1127
Eco130I CCWWGG 2 cut(s) 1107, 1784
Eco24I GRGCYC 1 cut(s) 1129
Eco47I GGWCC 2 cut(s) 1219, 1487
Eco53kI GAGCTC 1 cut(s) 1127
Eco57I CTGAAG 2 cut(s) 634, 669
Eco81I CCTNAGG 1 cut(s) 443
Eco88I CYCGRG 2 cut(s) 237, 432
EcoICRI GAGCTC 1 cut(s) 1127
EcoNI CCTNNNNNAGG 1 cut(s) 1037
EcoRI GAATTC 1 cut(s) 1708
EcoRII CCWGG 3 cut(s) 51, 589, 1356
EcoT14I CCWWGG 2 cut(s) 1107, 1784
EcoT22I ATGCAT 1 cut(s) 816
EcoT38I GRGCYC 1 cut(s) 1129
ErhI CCWWGG 2 cut(s) 1107, 1784
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 8 cut(s) 84, 200, 476, 499, 1237, 1282, 1307, 1433
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 2 cut(s) 1232, 1485
FatI CATG 8 cut(s) 80, 196, 472, 495, 1233, 1278, 1303, 1429
FauNDI CATATG 1 cut(s) 1059
FbaI TGATCA 2 cut(s) 291, 856
Fnu4HI GCNGC 8 cut(s) 94, 206, 554, 792, 1011, 1083, 1298, 1763
FokI GGATG 3 cut(s) 1135, 1195, 1758
FriOI GRGCYC 1 cut(s) 1129
Fsp4HI GCNGC 8 cut(s) 94, 206, 554, 792, 1011, 1083, 1298, 1763
FspBI CTAG 5 cut(s) 677, 1044, 1175, 1503, 1580
GlaI GCGC 2 cut(s) 264, 334
GluI GCNGC 8 cut(s) 94, 206, 554, 792, 1011, 1083, 1298, 1763
GsuI CTGGAG 3 cut(s) 573, 594, 1304
HaeII RGCGCY 1 cut(s) 266
HaeIII GGCC 5 cut(s) 8, 56, 170, 300, 736
HapII CCGG 3 cut(s) 1019, 1240, 1405
HgaI GACGC 2 cut(s) 70, 85
HhaI GCGC 2 cut(s) 265, 335
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 8 cut(s) 84, 200, 476, 499, 1237, 1282, 1307, 1433
Hin6I GCGC 2 cut(s) 263, 333
HinP1I GCGC 2 cut(s) 263, 333
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 2 cut(s) 12, 916
HinfI GANTC 9 cut(s) 77, 138, 504, 524, 937, 944, 1187, 1531, 1538
HpaII CCGG 3 cut(s) 1019, 1240, 1405
HphI GGTGA 4 cut(s) 9, 396, 638, 1808
Hpy166II GTNNAC 5 cut(s) 404, 998, 1067, 1231, 1333
Hpy188I TCNGA 6 cut(s) 133, 143, 906, 1100, 1165, 1758
Hpy188III TCNNGA 8 cut(s) 237, 432, 805, 941, 987, 1304, 1490, 1535
Hpy8I GTNNAC 5 cut(s) 404, 998, 1067, 1231, 1333
Hpy99I CGWCG 2 cut(s) 64, 101
HpyAV CCTTC 3 cut(s) 406, 1033, 1201
HpyCH4III ACNGT 3 cut(s) 21, 606, 900
HpyCH4IV ACGT 2 cut(s) 750, 1695
HpyF10VI GCNNNNNNNGC 7 cut(s) 167, 468, 562, 1019, 1091, 1279, 1621
HpySE526I ACGT 2 cut(s) 750, 1695
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 8 cut(s) 84, 200, 476, 499, 1237, 1282, 1307, 1433
HspAI GCGC 2 cut(s) 263, 333
Ksp22I TGATCA 2 cut(s) 291, 856
Kzo9I GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
LmnI GCTCC 7 cut(s) 118, 133, 239, 520, 770, 1278, 1359
Lsp1109I GCAGC 8 cut(s) 105, 192, 540, 803, 997, 1069, 1284, 1774
LweI GCATC 6 cut(s) 431, 801, 803, 1157, 1594, 1749
MaeI CTAG 5 cut(s) 677, 1044, 1175, 1503, 1580
MaeII ACGT 2 cut(s) 750, 1695
MaeIII GTNAC 1 cut(s) 1178
MalI GATC 8 cut(s) 293, 344, 803, 858, 1207, 1321, 1393, 1409
MbiI CCGCTC 1 cut(s) 113
MboI GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
MflI RGATCY 2 cut(s) 342, 1391
MhlI GDGCHC 2 cut(s) 1129, 1275
MlsI TGGCCA 1 cut(s) 736
MluCI AATT 6 cut(s) 463, 670, 1452, 1517, 1708, 1745
MluNI TGGCCA 1 cut(s) 736
MlyI GAGTC 7 cut(s) 86, 498, 533, 953, 1181, 1525, 1532
Mox20I TGGCCA 1 cut(s) 736
Mph1103I ATGCAT 1 cut(s) 816
MroXI GAANNNNTTC 2 cut(s) 917, 936
MscI TGGCCA 1 cut(s) 736
MseI TTAA 1 cut(s) 1791
MslI CAYNNNNRTG 1 cut(s) 1505
Msp20I TGGCCA 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 1010
MspI CCGG 3 cut(s) 1019, 1240, 1405
MspR9I CCNGG 3 cut(s) 53, 591, 1358
Mva1269I GAATGC 1 cut(s) 793
MvaI CCWGG 3 cut(s) 53, 591, 1358
MwoI GCNNNNNNNGC 7 cut(s) 167, 468, 562, 1019, 1091, 1279, 1621
NdeI CATATG 1 cut(s) 1059
NdeII GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
NheI GCTAGC 1 cut(s) 1579
NlaIII CATG 8 cut(s) 84, 200, 476, 499, 1237, 1282, 1307, 1433
NlaIV GGNNCC 3 cut(s) 344, 1005, 1220
NmeAIII GCCGAG 1 cut(s) 146
NsiI ATGCAT 1 cut(s) 816
NspI RCATGY 1 cut(s) 1282
PaeI GCATGC 1 cut(s) 1282
PagI TCATGA 1 cut(s) 1303
PcsI WCGNNNNNNNCGW 1 cut(s) 235
PctI GAATGC 1 cut(s) 793
PdmI GAANNNNTTC 2 cut(s) 917, 936
PfeI GAWTC 2 cut(s) 138, 937
PflMI CCANNNNNTGG 2 cut(s) 259, 413
PkrI GCNGC 8 cut(s) 95, 207, 555, 793, 1012, 1084, 1299, 1764
PleI GAGTC 7 cut(s) 85, 498, 532, 952, 1181, 1525, 1532
PpsI GAGTC 7 cut(s) 85, 498, 532, 952, 1181, 1525, 1532
Ppu21I YACGTR 1 cut(s) 751
Psp124BI GAGCTC 1 cut(s) 1129
Psp6I CCWGG 3 cut(s) 51, 589, 1356
PspGI CCWGG 3 cut(s) 51, 589, 1356
PspN4I GGNNCC 3 cut(s) 344, 1005, 1220
PspPI GGNCC 2 cut(s) 1219, 1487
PstNI CAGNNNCTG 3 cut(s) 797, 846, 904
PsuI RGATCY 2 cut(s) 342, 1391
PvuII CAGCTG 1 cut(s) 1010
RsaI GTAC 6 cut(s) 155, 615, 1066, 1116, 1385, 1399
RsaNI GTAC 6 cut(s) 154, 614, 1065, 1115, 1384, 1398
RseI CAYNNNNRTG 1 cut(s) 1505
SacI GAGCTC 1 cut(s) 1129
SaqAI TTAA 1 cut(s) 1791
SatI GCNGC 8 cut(s) 94, 206, 554, 792, 1011, 1083, 1298, 1763
Sau3AI GATC 8 cut(s) 291, 342, 801, 856, 1205, 1319, 1391, 1407
Sau96I GGNCC 2 cut(s) 1219, 1487
ScaI AGTACT 2 cut(s) 615, 1385
SchI GAGTC 7 cut(s) 86, 498, 533, 953, 1181, 1525, 1532
ScrFI CCNGG 3 cut(s) 53, 591, 1358
SduI GDGCHC 2 cut(s) 1129, 1275
SfaNI GCATC 6 cut(s) 431, 801, 803, 1157, 1594, 1749
SfcI CTRYAG 3 cut(s) 306, 1387, 1569
SinI GGWCC 2 cut(s) 1219, 1487
SmiMI CAYNNNNRTG 1 cut(s) 1505
SpeI ACTAGT 1 cut(s) 1502
SphI GCATGC 1 cut(s) 1282
Sse9I AATT 6 cut(s) 463, 670, 1452, 1517, 1708, 1745
SsiI CCGC 3 cut(s) 111, 1022, 1276
SspMI CTAG 5 cut(s) 677, 1044, 1175, 1503, 1580
SstI GAGCTC 1 cut(s) 1129
StyD4I CCNGG 3 cut(s) 51, 589, 1356
StyI CCWWGG 2 cut(s) 1107, 1784
TaaI ACNGT 3 cut(s) 21, 606, 900
TaiI ACGT 2 cut(s) 753, 1698
TaqI TCGA 5 cut(s) 59, 804, 852, 1458, 1712
TasI AATT 6 cut(s) 463, 670, 1452, 1517, 1708, 1745
TatI WGTACW 2 cut(s) 613, 1383
TfiI GAWTC 2 cut(s) 138, 937
Tru1I TTAA 1 cut(s) 1791
Tru9I TTAA 1 cut(s) 1791
TscAI CASTG 4 cut(s) 37, 192, 387, 1374
TseI GCWGC 8 cut(s) 93, 205, 553, 791, 1010, 1082, 1297, 1762
TspDTI ATGAA 4 cut(s) 185, 646, 969, 1250
TspGWI ACGGA 2 cut(s) 352, 885
TspRI CASTG 4 cut(s) 37, 192, 387, 1374
Van91I CCANNNNNTGG 2 cut(s) 259, 413
VpaK11BI GGWCC 2 cut(s) 1219, 1487
XagI CCTNNNNNAGG 1 cut(s) 1037
XapI RAATTY 3 cut(s) 1452, 1517, 1708
XceI RCATGY 1 cut(s) 1282
XmnI GAANNNNTTC 2 cut(s) 917, 936
XspI CTAG 5 cut(s) 677, 1044, 1175, 1503, 1580
ZrmI AGTACT 2 cut(s) 615, 1385
Zsp2I ATGCAT 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.