Rh2CG285200

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0011011
Physical Location & Seq
Reverse (-)
31985467 .. 31991911
6445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG285200.1

Sequence Viewer

Length: 2070 bp
ATGGAGGCCGAAGCTTCACCGTTTGAGAGCAGTGAGTTGGTGACGAGTTTCCTGGCGTCGACGCCTTTGCTAAGCGAGTCATGGAGGCTGTGCAGCGTCGCCAACGCCTCCGCTTCATTGGGTTTCGTATCGGAGCGAATCGGAGATGTCGGGTACTTTGCTTTCTCGGCCTGCAGTACCCAATTGATCACGGAGGGGTCGGAATACTACTCCACTTGCAGAAACTTGGTGCCATTGGATAGCGCAGGCGCCGGCACCGGTGACCGGCCTTTTGCGGTATTGACCAAAAGTAGTAACAGTAGTATCAATGGAGAAGAAGATGAGGGTGCAGTGATGGTTCATGCATGGCTGCTCAATCTCTTCTTGTTTTTTCGTAGCTCCCGAGCTTTCCAAGACCAGATGTTGCCGCTGATAAATGGAAGCAAGTCCATTGTGATCACAGGCCATTCAATAGGAGGAACAACTGCCTCACTCTGCGCTCTTTGGCTCCTTTGTTACCTGCAATCCGTCTCTTCTACCCTTTCGGTGCTTTGCATTACCTTCGGTTCACCATTACTTGGGAACGAATCGCTTTCCCGAGCAATCCTTAGGGAAAGATGGGGTGGCAACTTTTGCCATGTGGTGTCCAAGTATGACATCATGCCAAGACTCCTCTTCGCTCCTTTAGAGTCCTGCACTCGCCAACTGCATTTCTTGCTGCAATACTGGCAGATGTCTATGTTGGCTTCTTCTCCAGGCAACTTTGGACAGTCTCCAGTACTTCAACTCGGTGAGGAAGAGAAAGCTCAGCTCTTCAGCTTCATATCACATTGTAATTTGCTAGTGTCTTCAGTAGCACAAGCAGAAGCAAGAGAAGAGAAAGCAAATAGTTTGTATTGGCCATTTGGAAACTACGTGTTTTGCTCACAAGAGGGAGCTATTTGTGTGGAGAATGCAGCATCTGTGATCGAGATGATGCATTTGATGTTTATGACTGGTAATCCCAGTTGCTGTATCGATGATCATCTAAAATACGGACACTATGTTGGGAAGATTTCTTCACAGTTTCTGAATAAGAAAAGCTTCTTGCAAGGGGAAGTGATTCCTGAGTCAAGCTATGAAGCAGGGGTTGAACTGGCATTGCAGTCATCAGGAATAAGTAAACAGGAACCAGCTGCCAGACCGGCGGAAGATTGCCTAAAGAAGGCTAGGGGAATGAGAAATATGCGTACACCAAGCCTCAACGCTGCAAACTTAGCCATCAGATTATCCAAGGTTGTACCTTACAGAGCTCAAATAGAGTGGTATATGGCATCCTGTGATAAGGCTGATGAGCAACTAGGTTACTACGACTCCTTCAAGCAACGGGGATCATCCAAAAGGGACCACAAAGTCAACATGAACCGGCTCTTGCTTGCTGGATTTTGGAATGATGTGCTCCGCATGCTGCAGAACAACGAGCTGCCTCATGATTTTCACAAGCGATCAAAATGGGTAAACACTTCCCACTTTTACAAGCTCCTGGTAGAGCCACTGGACATTGCAGAGTATTATAGATCTGGTATGCACCGGATCAAGGGTCATTACTTGAAGCATGGAAGAGAGAGGAGGTTTGAGATTTTCGACAGGTGGTGGGGACAGAGAGAGGTTGGTCCAGAAGAAAACACTACTAGTACGAGAAGCAAATTTGCAGGTTTGACTCAGGACTCTTGCTTCTGGGCTAAGGTGGAGGAAGCTACAGAGTGGCTAGCTAATGTTAGAAGCGAGAGTGATGCAAGCAAGCTGGCTCTGCTGTGGGAAAATATCAATAAATTTGAAAGGTATGCAACCATATTAGTTGATCGCAAGGAGGTCTCTAAAGACGTTGTAGCAAAGAATTCGAGCTTTTCTAAGTGGGTAGAAGAGTTGAGAGAATTGAAATCTCAGATGCAGCAAATCCATCCTCAGTTCCCAAGGTTTAATGGTACGTGCATAATTCAATATTCTAAGGAATTGGATATTAAGAAGGGAGGAGGAAGACAAATTGGTATGAAAAAAATCCACAAGGAATTGGCCGGCTTAATTTCCCCAAATAAAATTTCCCGACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

689

Amino Acids

77.6

Weight (kDa)

8.03

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 109 - 219 1.8e-15 Lipase (class 3)
EDS1_EP PF18117 424 - 635 3.5e-72 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52430
fragaria_vesca FvH4_1g24940 FvH4_1g24940 FvH4_1g24940
malus_domestica MD15G1136300.v1.1
prunus_persica Prupe.4G276500_v2.0.a1 Prupe.4G276500_v2.0.a1
pyrus_communis pycom15g12290
rosa_chinensis RchiOBHm_Chr2g0122591
rosa_laevigata RLG00000018693
rosa_multiflora Rmu_sc0003133.1_g000001 Rmu_sc0010907.1_g000001
rosa_roxburghii Rroxscaffold_2G00121450
rosa_rugosa Rorug02G0239700 Rorug02G0239800
rosa_samantha Rh2AG298400 Rh2BG307100 Rh2CG285200 Rh2DG322000
rosa_wichuraiana Rw2G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1370
Acc36I ACCTGC 2 cut(s) 507, 1661
AccB1I GGYRCC 3 cut(s) 229, 248, 254
AccB7I CCANNNNNTGG 1 cut(s) 557
AccI GTMKAC 1 cut(s) 59
AciI CCGC 5 cut(s) 111, 275, 407, 1166, 1420
AclWI GGATC 2 cut(s) 1357, 1559
AcoI YGGCCR 2 cut(s) 878, 2031
AcsI RAATTY 4 cut(s) 1664, 1790, 1855, 2055
AcuI CTGAAG 2 cut(s) 778, 813
AcyI GRCGYC 3 cut(s) 56, 62, 249
AfaI GTAC 7 cut(s) 155, 178, 759, 1210, 1260, 1654, 1945
AfiI CCNNNNNNNGG 4 cut(s) 264, 557, 1183, 1555
AflIII ACRYGT 1 cut(s) 894
AgeI ACCGGT 1 cut(s) 257
AgsI TTSAA 8 cut(s) 450, 764, 1112, 1339, 1570, 1796, 1897, 1958
AhlI ACTAGT 1 cut(s) 1649
AjnI CCWGG 3 cut(s) 51, 733, 1500
AjuI GAANNNNNNNTTGG 1 cut(s) 2041
Alw21I GWGCWC 2 cut(s) 1273, 1419
Alw26I GTCTC 3 cut(s) 514, 756, 1837
AlwI GGATC 2 cut(s) 1357, 1559
AlwNI CAGNNNCTG 3 cut(s) 941, 990, 1048
Ama87I CYCGRG 2 cut(s) 381, 576
AoxI GGCC 6 cut(s) 6, 168, 266, 442, 878, 2031
ApeKI GCWGC 9 cut(s) 93, 349, 697, 935, 1154, 1226, 1426, 1441, 1909
ApoI RAATTY 4 cut(s) 1664, 1790, 1855, 2055
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AsiGI ACCGGT 1 cut(s) 257
Asp700I GAANNNNTTC 2 cut(s) 1061, 1080
AspLEI GCGC 3 cut(s) 245, 251, 479
AspS9I GGNCC 2 cut(s) 1363, 1631
AsuHPI GGTGA 5 cut(s) 9, 52, 272, 540, 782
AsuNHI GCTAGC 1 cut(s) 1726
AvaI CYCGRG 2 cut(s) 381, 576
AvaII GGWCC 2 cut(s) 1363, 1631
AxyI CCTNAGG 1 cut(s) 587
BaeI ACNNNNGTAYC 2 cut(s) 286, 319
BalI TGGCCA 1 cut(s) 880
BanI GGYRCC 3 cut(s) 229, 248, 254
BanII GRGCYC 1 cut(s) 1273
BbsI GAAGAC 2 cut(s) 819, 2002
Bbv12I GWGCWC 2 cut(s) 1273, 1419
BbvI GCAGC 9 cut(s) 105, 336, 684, 947, 1141, 1213, 1413, 1428, 1921
BccI CCATC 4 cut(s) 328, 591, 1247, 1926
BcgI CGANNNNNNTGC 8 cut(s) 49, 83, 523, 557, 1733, 1767, 1839, 1873
BciT130I CCWGG 3 cut(s) 53, 735, 1502
BclI TGATCA 3 cut(s) 186, 435, 1000
BcoDI GTCTC 3 cut(s) 514, 756, 1837
BcuI ACTAGT 1 cut(s) 1649
BfaI CTAG 5 cut(s) 821, 1188, 1319, 1650, 1727
BfmI CTRYAG 3 cut(s) 172, 1427, 1716
BfoI RGCGCY 1 cut(s) 252
BfuAI ACCTGC 2 cut(s) 507, 1661
BglI GCCNNNNNGGC 1 cut(s) 1163
BglII AGATCT 1 cut(s) 1535
BlpI GCTNAGC 2 cut(s) 71, 786
BmcAI AGTACT 1 cut(s) 759
Bme1390I CCNGG 3 cut(s) 53, 735, 1502
Bme18I GGWCC 2 cut(s) 1363, 1631
BmeT110I CYCGRG 2 cut(s) 381, 576
BmgT120I GGNCC 2 cut(s) 1363, 1631
BmiI GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
BmrFI CCNGG 3 cut(s) 53, 735, 1502
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 5 cut(s) 945, 947, 1301, 1741, 1896
BmtI GCTAGC 1 cut(s) 1730
BmuI ACTGGG 1 cut(s) 978
BpiI GAAGAC 2 cut(s) 819, 2002
BpmI CTGGAG 2 cut(s) 717, 738
Bpu10I CCTNAGC 1 cut(s) 1701
Bpu1102I GCTNAGC 2 cut(s) 71, 786
Bsa29I ATCGAT 1 cut(s) 996
BsaAI YACGTR 2 cut(s) 895, 1947
BsaBI GATNNNNATC 1 cut(s) 1002
BsaHI GRCGYC 3 cut(s) 56, 62, 249
BsaI GGTCTC 1 cut(s) 1837
BsaJI CCNNGG 2 cut(s) 1251, 1931
BsaWI WCCGGW 2 cut(s) 257, 1548
BsaXI ACNNNNNCTCC 2 cut(s) 1316, 1346
Bsc4I CCNNNNNNNGG 4 cut(s) 264, 557, 1183, 1555
Bse118I RCCGGY 6 cut(s) 251, 257, 264, 1162, 1383, 2033
Bse1I ACTGG 6 cut(s) 710, 755, 979, 984, 1119, 1518
Bse21I CCTNAGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 1118, 1518
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 3 cut(s) 53, 735, 1502
BseCI ATCGAT 1 cut(s) 996
BseDI CCNNGG 2 cut(s) 1251, 1931
BseGI GGATG 3 cut(s) 1292, 1352, 1918
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 4 cut(s) 264, 557, 1183, 1555
BseMI GCAATG 2 cut(s) 1118, 1518
BseMII CTCAG 5 cut(s) 800, 1077, 1694, 1916, 1937
BseNI ACTGG 6 cut(s) 710, 755, 979, 984, 1119, 1518
BseRI GAGGAG 3 cut(s) 641, 1600, 2004
BseXI GCAGC 9 cut(s) 105, 336, 684, 947, 1141, 1213, 1413, 1428, 1921
BsgI GTGCAG 3 cut(s) 112, 348, 658
BshFI GGCC 6 cut(s) 8, 170, 268, 444, 880, 2033
BshNI GGYRCC 3 cut(s) 229, 248, 254
BshTI ACCGGT 1 cut(s) 257
BshVI ATCGAT 1 cut(s) 996
BsiHKAI GWGCWC 2 cut(s) 1273, 1419
BsiHKCI CYCGRG 2 cut(s) 381, 576
BsiSI CCGG 7 cut(s) 252, 258, 265, 1163, 1384, 1549, 2034
BslFI GGGAC 2 cut(s) 1376, 1629
BslI CCNNNNNNNGG 4 cut(s) 264, 557, 1183, 1555
BsmAI GTCTC 3 cut(s) 514, 756, 1837
BsmBI CGTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 1376, 1629
BsmI GAATGC 1 cut(s) 937
BsnI GGCC 6 cut(s) 8, 170, 268, 444, 880, 2033
Bso31I GGTCTC 1 cut(s) 1837
BsoBI CYCGRG 2 cut(s) 381, 576
Bsp1286I GDGCHC 2 cut(s) 1273, 1419
Bsp143I GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
Bsp1720I GCTNAGC 2 cut(s) 71, 786
BspACI CCGC 5 cut(s) 111, 275, 407, 1166, 1420
BspANI GGCC 6 cut(s) 8, 170, 268, 444, 880, 2033
BspCNI CTCAG 5 cut(s) 799, 1078, 1693, 1915, 1936
BspDI ATCGAT 1 cut(s) 996
BspHI TCATGA 1 cut(s) 1447
BspLI GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
BspMAI CTGCAG 2 cut(s) 176, 1431
BspMI ACCTGC 2 cut(s) 507, 1661
BspOI GCTAGC 1 cut(s) 1730
BspPI GGATC 2 cut(s) 1357, 1559
BspQI GCTCTTC 1 cut(s) 797
BspT107I GGYRCC 3 cut(s) 229, 248, 254
BspTNI GGTCTC 1 cut(s) 1837
BsrDI GCAATG 2 cut(s) 1118, 1518
BsrFI RCCGGY 6 cut(s) 251, 257, 264, 1162, 1383, 2033
BsrI ACTGG 6 cut(s) 710, 755, 979, 984, 1119, 1518
BssAI RCCGGY 6 cut(s) 251, 257, 264, 1162, 1383, 2033
BssECI CCNNGG 2 cut(s) 1251, 1931
BssMI GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
BssNI GRCGYC 3 cut(s) 56, 62, 249
BssT1I CCWWGG 2 cut(s) 1251, 1931
Bst2UI CCWGG 3 cut(s) 53, 735, 1502
Bst4CI ACNGT 4 cut(s) 21, 299, 750, 1044
Bst6I CTCTTC 8 cut(s) 365, 517, 659, 771, 797, 849, 1573, 1875
BstACI GRCGYC 3 cut(s) 56, 62, 249
BstAPI GCANNNNNTGC 2 cut(s) 612, 694
BstBAI YACGTR 2 cut(s) 895, 1947
BstEII GGTNACC 1 cut(s) 260
BstENI CCTNNNNNAGG 1 cut(s) 1181
BstF5I GGATG 3 cut(s) 1292, 1352, 1918
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 3 cut(s) 245, 251, 479
BstKTI GATC 9 cut(s) 189, 438, 948, 1003, 1352, 1466, 1538, 1554, 1822
BstMAI GTCTC 3 cut(s) 514, 756, 1837
BstMBI GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
BstMWI GCNNNNNNNGC 8 cut(s) 167, 612, 694, 706, 1163, 1235, 1423, 1768
BstNI CCWGG 3 cut(s) 53, 735, 1502
BstNSI RCATGY 1 cut(s) 1426
BstPI GGTNACC 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 51, 733, 1500
BstSFI CTRYAG 3 cut(s) 172, 1427, 1716
BstV1I GCAGC 9 cut(s) 105, 336, 684, 947, 1141, 1213, 1413, 1428, 1921
BstV2I GAAGAC 2 cut(s) 819, 2002
BstX2I RGATCY 1 cut(s) 1535
BstYI RGATCY 1 cut(s) 1535
Bsu15I ATCGAT 1 cut(s) 996
Bsu36I CCTNAGG 1 cut(s) 587
BsuRI GGCC 6 cut(s) 8, 170, 268, 444, 880, 2033
BsuTUI ATCGAT 1 cut(s) 996
BtsCI GGATG 3 cut(s) 1292, 1352, 1918
BtsI GCAGTG 2 cut(s) 37, 336
BtsIMutI CAGTG 3 cut(s) 37, 336, 1511
BveI ACCTGC 2 cut(s) 507, 1661
CaiI CAGNNNCTG 3 cut(s) 941, 990, 1048
CciI TCATGA 1 cut(s) 1447
CfoI GCGC 3 cut(s) 245, 251, 479
Cfr10I RCCGGY 6 cut(s) 251, 257, 264, 1162, 1383, 2033
Cfr13I GGNCC 2 cut(s) 1363, 1631
ClaI ATCGAT 1 cut(s) 996
CseI GACGC 3 cut(s) 45, 70, 85
Csp6I GTAC 7 cut(s) 154, 177, 758, 1209, 1259, 1653, 1944
CspAI ACCGGT 1 cut(s) 257
CspCI CAANNNNNGTGG 2 cut(s) 1262, 1297
CviAII CATG 9 cut(s) 81, 341, 345, 617, 640, 1378, 1423, 1448, 1574
CviQI GTAC 7 cut(s) 154, 177, 758, 1209, 1259, 1653, 1944
DinI GGCGCC 1 cut(s) 250
DpnI GATC 9 cut(s) 188, 437, 947, 1002, 1351, 1465, 1537, 1553, 1821
DpnII GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
DrdI GACNNNNNNGTC 1 cut(s) 1370
DseDI GACNNNNNNGTC 1 cut(s) 1370
EaeI YGGCCR 2 cut(s) 878, 2031
Eam1104I CTCTTC 8 cut(s) 365, 517, 659, 771, 797, 849, 1573, 1875
EarI CTCTTC 8 cut(s) 365, 517, 659, 771, 797, 849, 1573, 1875
EciI GGCGGA 1 cut(s) 1181
Ecl136II GAGCTC 1 cut(s) 1271
Eco130I CCWWGG 2 cut(s) 1251, 1931
Eco24I GRGCYC 1 cut(s) 1273
Eco31I GGTCTC 1 cut(s) 1837
Eco47I GGWCC 2 cut(s) 1363, 1631
Eco53kI GAGCTC 1 cut(s) 1271
Eco57I CTGAAG 2 cut(s) 778, 813
Eco81I CCTNAGG 1 cut(s) 587
Eco88I CYCGRG 2 cut(s) 381, 576
Eco91I GGTNACC 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 1271
EcoNI CCTNNNNNAGG 1 cut(s) 1181
EcoO65I GGTNACC 1 cut(s) 260
EcoRI GAATTC 1 cut(s) 1855
EcoRII CCWGG 3 cut(s) 51, 733, 1500
EcoT14I CCWWGG 2 cut(s) 1251, 1931
EcoT22I ATGCAT 2 cut(s) 346, 960
EcoT38I GRGCYC 1 cut(s) 1273
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
ErhI CCWWGG 2 cut(s) 1251, 1931
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 9 cut(s) 84, 344, 348, 620, 643, 1381, 1426, 1451, 1577
FalI AAGNNNNNCTT 2 cut(s) 1046, 1078
FaqI GGGAC 2 cut(s) 1376, 1629
FatI CATG 9 cut(s) 80, 340, 344, 616, 639, 1377, 1422, 1447, 1573
FbaI TGATCA 3 cut(s) 186, 435, 1000
FblI GTMKAC 1 cut(s) 59
FokI GGATG 3 cut(s) 1279, 1339, 1905
FriOI GRGCYC 1 cut(s) 1273
FspBI CTAG 5 cut(s) 821, 1188, 1319, 1650, 1727
GlaI GCGC 3 cut(s) 244, 250, 478
GsuI CTGGAG 2 cut(s) 717, 738
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 6 cut(s) 8, 170, 268, 444, 880, 2033
HapII CCGG 7 cut(s) 252, 258, 265, 1163, 1384, 1549, 2034
HgaI GACGC 3 cut(s) 45, 70, 85
HhaI GCGC 3 cut(s) 245, 251, 479
Hin1I GRCGYC 3 cut(s) 56, 62, 249
Hin1II CATG 9 cut(s) 84, 344, 348, 620, 643, 1381, 1426, 1451, 1577
Hin6I GCGC 3 cut(s) 243, 249, 477
HinP1I GCGC 3 cut(s) 243, 249, 477
HincII GTYRAC 2 cut(s) 60, 1375
HindII GTYRAC 2 cut(s) 60, 1375
HindIII AAGCTT 2 cut(s) 12, 1060
HpaII CCGG 7 cut(s) 252, 258, 265, 1163, 1384, 1549, 2034
HphI GGTGA 5 cut(s) 9, 52, 272, 540, 782
Hpy166II GTNNAC 6 cut(s) 60, 548, 1142, 1211, 1375, 1477
Hpy188I TCNGA 6 cut(s) 133, 143, 202, 1050, 1244, 1905
Hpy188III TCNNGA 9 cut(s) 381, 576, 949, 1085, 1131, 1448, 1634, 1682, 2061
Hpy8I GTNNAC 6 cut(s) 60, 548, 1142, 1211, 1375, 1477
Hpy99I CGWCG 3 cut(s) 61, 64, 101
HpyAV CCTTC 4 cut(s) 550, 1177, 1345, 1978
HpyCH4III ACNGT 4 cut(s) 21, 299, 750, 1044
HpyCH4IV ACGT 3 cut(s) 894, 1842, 1946
HpyF10VI GCNNNNNNNGC 8 cut(s) 167, 612, 694, 706, 1163, 1235, 1423, 1768
HpySE526I ACGT 3 cut(s) 894, 1842, 1946
Hsp92I GRCGYC 3 cut(s) 56, 62, 249
Hsp92II CATG 9 cut(s) 84, 344, 348, 620, 643, 1381, 1426, 1451, 1577
HspAI GCGC 3 cut(s) 243, 249, 477
KasI GGCGCC 1 cut(s) 248
KroI GCCGGC 2 cut(s) 251, 2033
KroNI GCCGGC 2 cut(s) 253, 2035
Ksp22I TGATCA 3 cut(s) 186, 435, 1000
Kzo9I GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
LguI GCTCTTC 1 cut(s) 797
LmnI GCTCC 7 cut(s) 133, 383, 492, 664, 914, 1422, 1503
Lsp1109I GCAGC 9 cut(s) 105, 336, 684, 947, 1141, 1213, 1413, 1428, 1921
LweI GCATC 5 cut(s) 945, 947, 1301, 1741, 1896
MaeI CTAG 5 cut(s) 821, 1188, 1319, 1650, 1727
MaeII ACGT 3 cut(s) 894, 1842, 1946
MaeIII GTNAC 5 cut(s) 40, 260, 293, 494, 1322
MalI GATC 9 cut(s) 188, 437, 947, 1002, 1351, 1465, 1537, 1553, 1821
MboI GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
MfeI CAATTG 1 cut(s) 182
MflI RGATCY 1 cut(s) 1535
MhlI GDGCHC 2 cut(s) 1273, 1419
MlsI TGGCCA 1 cut(s) 880
MluNI TGGCCA 1 cut(s) 880
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 7 cut(s) 86, 642, 677, 1097, 1325, 1672, 1679
MmeI TCCRAC 1 cut(s) 180
Mox20I TGGCCA 1 cut(s) 880
Mph1103I ATGCAT 2 cut(s) 346, 960
MroNI GCCGGC 2 cut(s) 251, 2033
MroXI GAANNNNTTC 2 cut(s) 1061, 1080
MscI TGGCCA 1 cut(s) 880
MseI TTAA 3 cut(s) 1938, 1980, 2039
Msp20I TGGCCA 1 cut(s) 880
MspA1I CMGCKG 2 cut(s) 409, 1154
MspI CCGG 7 cut(s) 252, 258, 265, 1163, 1384, 1549, 2034
MspR9I CCNGG 3 cut(s) 53, 735, 1502
MunI CAATTG 1 cut(s) 182
Mva1269I GAATGC 1 cut(s) 937
MvaI CCWGG 3 cut(s) 53, 735, 1502
MwoI GCNNNNNNNGC 8 cut(s) 167, 612, 694, 706, 1163, 1235, 1423, 1768
NaeI GCCGGC 2 cut(s) 253, 2035
NarI GGCGCC 1 cut(s) 249
NdeII GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
NgoMIV GCCGGC 2 cut(s) 251, 2033
NheI GCTAGC 1 cut(s) 1726
NlaIII CATG 9 cut(s) 84, 344, 348, 620, 643, 1381, 1426, 1451, 1577
NlaIV GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 2 cut(s) 40, 260
NsiI ATGCAT 2 cut(s) 346, 960
NspI RCATGY 1 cut(s) 1426
PaeI GCATGC 1 cut(s) 1426
PagI TCATGA 1 cut(s) 1447
PciSI GCTCTTC 1 cut(s) 797
PcsI WCGNNNNNNNCGW 1 cut(s) 379
PctI GAATGC 1 cut(s) 937
PdiI GCCGGC 2 cut(s) 253, 2035
PdmI GAANNNNTTC 2 cut(s) 1061, 1080
PfeI GAWTC 3 cut(s) 138, 566, 1081
PflMI CCANNNNNTGG 1 cut(s) 557
PinAI ACCGGT 1 cut(s) 257
PleI GAGTC 7 cut(s) 85, 642, 676, 1096, 1325, 1672, 1679
PluTI GGCGCC 1 cut(s) 252
PpsI GAGTC 7 cut(s) 85, 642, 676, 1096, 1325, 1672, 1679
Ppu21I YACGTR 2 cut(s) 895, 1947
Psp124BI GAGCTC 1 cut(s) 1273
Psp6I CCWGG 3 cut(s) 51, 733, 1500
PspEI GGTNACC 1 cut(s) 260
PspGI CCWGG 3 cut(s) 51, 733, 1500
PspN4I GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
PspPI GGNCC 2 cut(s) 1363, 1631
PstI CTGCAG 2 cut(s) 176, 1431
PstNI CAGNNNCTG 3 cut(s) 941, 990, 1048
PsuI RGATCY 1 cut(s) 1535
PvuII CAGCTG 1 cut(s) 1154
RsaI GTAC 7 cut(s) 155, 178, 759, 1210, 1260, 1654, 1945
RsaNI GTAC 7 cut(s) 154, 177, 758, 1209, 1259, 1653, 1944
SacI GAGCTC 1 cut(s) 1273
SalI GTCGAC 1 cut(s) 58
SapI GCTCTTC 1 cut(s) 797
SaqAI TTAA 3 cut(s) 1938, 1980, 2039
Sau3AI GATC 9 cut(s) 186, 435, 945, 1000, 1349, 1463, 1535, 1551, 1819
Sau96I GGNCC 2 cut(s) 1363, 1631
ScaI AGTACT 1 cut(s) 759
SchI GAGTC 7 cut(s) 86, 642, 677, 1097, 1325, 1672, 1679
ScrFI CCNGG 3 cut(s) 53, 735, 1502
SduI GDGCHC 2 cut(s) 1273, 1419
SfaNI GCATC 5 cut(s) 945, 947, 1301, 1741, 1896
SfcI CTRYAG 3 cut(s) 172, 1427, 1716
SfoI GGCGCC 1 cut(s) 250
SgrAI CRCCGGYG 1 cut(s) 257
SgrDI CGTCGACG 1 cut(s) 58
SinI GGWCC 2 cut(s) 1363, 1631
SpeI ACTAGT 1 cut(s) 1649
SphI GCATGC 1 cut(s) 1426
SsiI CCGC 5 cut(s) 111, 275, 407, 1166, 1420
SspDI GGCGCC 1 cut(s) 248
SspI AATATT 1 cut(s) 1961
SspMI CTAG 5 cut(s) 821, 1188, 1319, 1650, 1727
SstI GAGCTC 1 cut(s) 1273
StyD4I CCNGG 3 cut(s) 51, 733, 1500
StyI CCWWGG 2 cut(s) 1251, 1931
TaaI ACNGT 4 cut(s) 21, 299, 750, 1044
TaiI ACGT 3 cut(s) 897, 1845, 1949
TaqI TCGA 5 cut(s) 59, 948, 996, 1602, 1859
TatI WGTACW 1 cut(s) 757
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 3 cut(s) 138, 566, 1081
Tru1I TTAA 3 cut(s) 1938, 1980, 2039
Tru9I TTAA 3 cut(s) 1938, 1980, 2039
TscAI CASTG 3 cut(s) 37, 336, 1518
TseFI GTSAC 2 cut(s) 40, 260
TseI GCWGC 9 cut(s) 93, 349, 697, 935, 1154, 1226, 1426, 1441, 1909
Tsp45I GTSAC 2 cut(s) 40, 260
TspDTI ATGAA 6 cut(s) 105, 329, 790, 1113, 1394, 2024
TspGWI ACGGA 3 cut(s) 206, 496, 1029
TspRI CASTG 3 cut(s) 37, 336, 1518
Van91I CCANNNNNTGG 1 cut(s) 557
VpaK11BI GGWCC 2 cut(s) 1363, 1631
XagI CCTNNNNNAGG 1 cut(s) 1181
XapI RAATTY 4 cut(s) 1664, 1790, 1855, 2055
XceI RCATGY 1 cut(s) 1426
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 2 cut(s) 1061, 1080
XspI CTAG 5 cut(s) 821, 1188, 1319, 1650, 1727
ZrmI AGTACT 1 cut(s) 759
Zsp2I ATGCAT 2 cut(s) 346, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.