pycom15g12290

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0011011
Physical Location & Seq
Forward (+)
8400688 .. 8408249
7562 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g12290.4

Sequence Viewer

Length: 1986 bp
ATGTATGTATATATGTATTCAAATTCCATTCAAACTCTGGCCGGTCACTTTAAAGACCCCAAAAGAAGAAAAATAAGAAAAGAAGAACTTCTTGCTAGTCAGTCCATGGATGCCGAAGCTTCACCGTTTGAGAGCAGTGAGACAGTTGCGAGTTTCTTGGCATCGACTCCCTTGCTGATTGAGTCATGGAGGCTATGCAGCGTAGCAAACACCACTCCCGGCGCTGCTTTCGTATCCCATCAAATCGGAGACGTCGGATATTTTGCTTTATCCGGAGAAGAAGATCAATGTTTGAGTTTCAGAAATTTGGTAGCGTTGGAGAACGCCGGCCACGGCCTTTTTCCACCATTATCCAACAACAACAATAATAATCATAATAATATTATTAATGGAGAGGAAGAGGCCGTCATGGTGCATGCAGGGCTGCTCAAGCTCTTCGTGTCTCTGCGCTTCTCCCAAACTTTCCAAGACCAGATGTCGGCCCTAACAGAGAAAAGCAAGTCCATTGTGATCACAGGCCATTCTATAGGAGGAACAACTGCCTCACTCTGTGCTCTCTGGCTCCTTTCTTCCCTCCAATCTGTCTCTTCCACCCTTTCAGTGCTTTGCATTACCTTTGGATCACCATTGCTCGGCAATGCCTGCCTTTCGCGAGCAATCCTCCGAGAAAGATGGGGTGGCAATTTTTTCCACGTAGTTTCCAAGTACGACATCATGCCAAGACTACTCCTTGCTCCAATTGAATCATGCAGCGCTCAAGTACTGCATTTCTTACTACAATGCTTTCAGCACTTTCGAAATCTTGGTGTTCATGTTCAACTGCGCGACGAAGAGAAAGCTGCACTATCCAGCTTCGTGTCAGCTTATTTGCGTGCGTCATCATTGCAAGAAGGAGAAGAGAGAAAAAGCAGCAGCAGCATGTCGTATTGTCCATTTGGGAGCTACTTGTTTTGCTCACAAGAGGGCGCTGTGTGTGTGGAGAATGCAGTGTCTGTGATGACAATGATGCATTTGATGTTTATGCCAGCTGAAGCTAACCCAATGTGCTGTATCCAGGATCATCTCAACTACGGAGATTATGTTGACAAGATTTCTTCACAGTTTCTGAAGACAAGCTTCATGCAGCAGGATGAGCTTCCAGAATCAAGCTACGAAGCAGGGGTTGCATTGGCATTACAGTCCTTGGGAATAAATAGCCACGAACCAGCTGCCGCACCTGCGAAAGAGTGCCTAAAGATAGCAAGGCGATTAGGACGTACCCCAAGTTTGAATTGTGCTAATTTAGCTGTTCGGCTCTCCAAGTACACGCCTCACAGAGCTGAAATCGAGTGGTACATGGCATCCTGTGACGAGTCTGGTGAGCAACTGGGGTACTATGACGCCTTCAAGCATAGGGGCTCCTCAAAAAAGGGTAACAGGGTCAACATGAACCGCCTCATGCTTGCTGCATTTTGGGATGGGGTGATAAAAATGATGGAGACAAATGAGCTGCCTCACGATTTTCACAGGCGAGCAAAATGGGTAAATGCATCCCAGTTTTACATGCTCCTCGTTGAGCCATTGGACATCGCGGAGTACTATAGAACTGGTATGCACCATGTCAAGGGTCATTACTTGGCGCATGGAAGAGAGAGGAGGTTTGAAATATTCGACAGATGGTGGAAGGAGAGAAGCATCAACGATCAGAAGCAAAGTAGAAGCAGAAGCAAGTCTGCAGGTTTGACTCAGGATTCGTGCTTCTGGGCAAAGGTGGAGGAAGCTAGGGAATGGCTTACTAATTTTAGAAGTGAGACTGATACAAGGAAGCTACATTCTCTGTGGGAAAGTATCAATAACTTCGACAGCTATGCAGCCGGTTTGGTCCAGAGAAAGGAGGTCTCTAAAGACGTTATGGCAAAGAACTCAAGCTACATGAAATGGCTGCAAGAGTTGGCAGATCTGAGATCGCAGGTACGTCACGTCCAGCTTCAAATTCCAAAGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

662

Amino Acids

74.68

Weight (kDa)

7.31

Isoelectric Point (pI)

59.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52430
fragaria_vesca FvH4_1g24940 FvH4_1g24940 FvH4_1g24940
malus_domestica MD15G1136300.v1.1
prunus_persica Prupe.4G276500_v2.0.a1 Prupe.4G276500_v2.0.a1
pyrus_communis pycom15g12290
rosa_chinensis RchiOBHm_Chr2g0122591
rosa_laevigata RLG00000018693
rosa_multiflora Rmu_sc0003133.1_g000001 Rmu_sc0010907.1_g000001
rosa_roxburghii Rroxscaffold_2G00121450
rosa_rugosa Rorug02G0239700 Rorug02G0239800
rosa_samantha Rh2AG298400 Rh2BG307100 Rh2CG285200 Rh2DG322000
rosa_wichuraiana Rw2G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1225
AatII GACGTC 1 cut(s) 255
Acc36I ACCTGC 3 cut(s) 1225, 1706, 1939
AccII CGCG 3 cut(s) 652, 825, 1571
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 3 cut(s) 1212, 1432, 1571
AclWI GGATC 2 cut(s) 628, 1065
AcoI YGGCCR 2 cut(s) 39, 328
AcsI RAATTY 3 cut(s) 22, 304, 1971
AcuI CTGAAG 2 cut(s) 1050, 1127
AcyI GRCGYC 2 cut(s) 252, 1380
AdeI CACNNNGTG 1 cut(s) 551
AfaI GTAC 8 cut(s) 707, 762, 1258, 1304, 1334, 1373, 1577, 1953
AfeI AGCGCT 1 cut(s) 754
AfiI CCNNNNNNNGG 4 cut(s) 478, 632, 1603, 1870
AgsI TTSAA 8 cut(s) 21, 32, 743, 818, 1270, 1387, 1643, 1970
AhdI GACNNNNNGTC 1 cut(s) 475
AjiI CACGTC 1 cut(s) 1960
AjnI CCWGG 1 cut(s) 1053
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 7 cut(s) 134, 243, 447, 589, 1472, 1784, 1882
AlwI GGATC 2 cut(s) 628, 1065
AlwNI CAGNNNCTG 2 cut(s) 992, 1105
Aor13HI TCCGGA 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 754
AoxI GGCC 6 cut(s) 39, 328, 334, 402, 480, 517
ApoI RAATTY 3 cut(s) 22, 304, 1971
ArsI GACNNNNNNTTYG 2 cut(s) 1714, 1746
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 87
AspLEI GCGC 6 cut(s) 224, 450, 755, 825, 968, 1621
AspS9I GGNCC 2 cut(s) 481, 1861
AsuC2I CCSGG 1 cut(s) 219
AsuHPI GGTGA 4 cut(s) 114, 615, 1370, 1474
AsuII TTCGAA 1 cut(s) 796
AvaII GGWCC 1 cut(s) 1861
BanII GRGCYC 1 cut(s) 1400
BbsI GAAGAC 1 cut(s) 1115
Bbv12I GWGCWC 1 cut(s) 556
BccI CCATC 5 cut(s) 246, 666, 1451, 1468, 1650
BceAI ACGGC 2 cut(s) 349, 389
BcgI CGANNNNNNTGC 6 cut(s) 154, 188, 1190, 1224, 1829, 1863
BciT130I CCWGG 1 cut(s) 1055
BciVI GTATCC 2 cut(s) 244, 1061
BclI TGATCA 1 cut(s) 510
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 7 cut(s) 134, 243, 447, 589, 1472, 1784, 1882
BfaI CTAG 2 cut(s) 96, 1761
BfmI CTRYAG 3 cut(s) 525, 1579, 1713
BfoI RGCGCY 3 cut(s) 225, 756, 969
BfuAI ACCTGC 3 cut(s) 1225, 1706, 1939
BfuI GTATCC 2 cut(s) 244, 1061
BglII AGATCT 1 cut(s) 1936
BmcAI AGTACT 2 cut(s) 762, 1577
Bme1390I CCNGG 2 cut(s) 219, 1055
Bme18I GGWCC 1 cut(s) 1861
BmeRI GACNNNNNGTC 1 cut(s) 475
BmgBI CACGTC 1 cut(s) 1960
BmgT120I GGNCC 2 cut(s) 481, 1861
BmiI GGNNCC 2 cut(s) 563, 1399
BmrFI CCNGG 2 cut(s) 219, 1055
BmrI ACTGGG 2 cut(s) 1376, 1528
BmsI GCATC 6 cut(s) 100, 170, 996, 1349, 1538, 1683
BmuI ACTGGG 2 cut(s) 1376, 1528
BpiI GAAGAC 1 cut(s) 1115
BplI GAGNNNNNCTC 2 cut(s) 645, 677
Bpu14I TTCGAA 1 cut(s) 796
BpuEI CTTGAG 3 cut(s) 413, 741, 1888
BpuMI CCSGG 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 694
BsaHI GRCGYC 2 cut(s) 252, 1380
BsaI GGTCTC 1 cut(s) 1882
BsaJI CCNNGG 3 cut(s) 105, 331, 1182
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 1065, 1095
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 632, 1603, 1870
Bse118I RCCGGY 3 cut(s) 41, 326, 1853
Bse1I ACTGG 3 cut(s) 1371, 1534, 1591
Bse3DI GCAATG 3 cut(s) 626, 643, 881
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 1 cut(s) 1055
BseDI CCNNGG 3 cut(s) 105, 331, 1182
BseGI GGATG 5 cut(s) 115, 1135, 1340, 1462, 1529
BseLI CCNNNNNNNGG 4 cut(s) 478, 632, 1603, 1870
BseMI GCAATG 3 cut(s) 626, 643, 881
BseMII CTCAG 2 cut(s) 1739, 1931
BseNI ACTGG 3 cut(s) 1371, 1534, 1591
BseRI GAGGAG 3 cut(s) 1390, 1538, 1648
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 3 cut(s) 652, 825, 1571
BshFI GGCC 6 cut(s) 41, 330, 336, 404, 482, 519
BsiHKAI GWGCWC 1 cut(s) 556
BsiSI CCGG 5 cut(s) 42, 219, 273, 327, 1854
BslI CCNNNNNNNGG 4 cut(s) 478, 632, 1603, 1870
BsmAI GTCTC 7 cut(s) 134, 243, 447, 589, 1472, 1784, 1882
BsmBI CGTCTC 1 cut(s) 243
BsmI GAATGC 1 cut(s) 988
BsnI GGCC 6 cut(s) 41, 330, 336, 404, 482, 519
Bso31I GGTCTC 1 cut(s) 1882
Bsp119I TTCGAA 1 cut(s) 796
Bsp1286I GDGCHC 2 cut(s) 556, 1400
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
Bsp19I CCATGG 1 cut(s) 105
Bsp68I TCGCGA 1 cut(s) 652
BspACI CCGC 3 cut(s) 1212, 1432, 1571
BspANI GGCC 6 cut(s) 41, 330, 336, 404, 482, 519
BspCNI CTCAG 2 cut(s) 1738, 1932
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 3 cut(s) 652, 825, 1571
BspLI GGNNCC 2 cut(s) 563, 1399
BspMAI CTGCAG 1 cut(s) 1717
BspMI ACCTGC 3 cut(s) 1225, 1706, 1939
BspPI GGATC 2 cut(s) 628, 1065
BspQI GCTCTTC 1 cut(s) 440
BspT104I TTCGAA 1 cut(s) 796
BspTNI GGTCTC 1 cut(s) 1882
BsrDI GCAATG 3 cut(s) 626, 643, 881
BsrFI RCCGGY 3 cut(s) 41, 326, 1853
BsrI ACTGG 3 cut(s) 1371, 1534, 1591
BssAI RCCGGY 3 cut(s) 41, 326, 1853
BssECI CCNNGG 3 cut(s) 105, 331, 1182
BssMI GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
BssNI GRCGYC 2 cut(s) 252, 1380
BssT1I CCWWGG 2 cut(s) 105, 1182
Bst2UI CCWGG 1 cut(s) 1055
Bst4CI ACNGT 4 cut(s) 126, 145, 1101, 1179
Bst6I CTCTTC 6 cut(s) 393, 440, 592, 825, 891, 1621
BstACI GRCGYC 2 cut(s) 252, 1380
BstAPI GCANNNNNTGC 2 cut(s) 642, 1163
BstBAI YACGTR 1 cut(s) 694
BstBI TTCGAA 1 cut(s) 796
BstC8I GCNNGC 8 cut(s) 328, 417, 643, 654, 873, 1026, 1443, 1512
BstDEI CTNAG 3 cut(s) 1725, 1940, 1983
BstDSI CCRYGG 2 cut(s) 105, 331
BstF5I GGATG 5 cut(s) 115, 1135, 1340, 1462, 1529
BstFNI CGCG 3 cut(s) 652, 825, 1571
BstH2I RGCGCY 3 cut(s) 225, 756, 969
BstHHI GCGC 6 cut(s) 224, 450, 755, 825, 968, 1621
BstKTI GATC 7 cut(s) 286, 513, 623, 1060, 1684, 1939, 1946
BstMAI GTCTC 7 cut(s) 134, 243, 447, 589, 1472, 1784, 1882
BstMBI GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
BstMWI GCNNNNNNNGC 8 cut(s) 421, 430, 642, 915, 1132, 1163, 1217, 1283
BstNI CCWGG 1 cut(s) 1055
BstNSI RCATGY 3 cut(s) 419, 922, 1546
BstSCI CCNGG 2 cut(s) 217, 1053
BstSFI CTRYAG 3 cut(s) 525, 1579, 1713
BstUI CGCG 3 cut(s) 652, 825, 1571
BstV2I GAAGAC 1 cut(s) 1115
BstX2I RGATCY 1 cut(s) 1936
BstYI RGATCY 1 cut(s) 1936
BsuI GTATCC 2 cut(s) 244, 1061
BsuRI GGCC 6 cut(s) 41, 330, 336, 404, 482, 519
BtgI CCRYGG 2 cut(s) 105, 331
BtgZI GCGATG 1 cut(s) 1552
BtrI CACGTC 1 cut(s) 1960
BtsCI GGATG 5 cut(s) 115, 1135, 1340, 1462, 1529
BtsI GCAGTG 2 cut(s) 142, 993
BtsIMutI CAGTG 3 cut(s) 142, 606, 993
BtuMI TCGCGA 1 cut(s) 652
BveI ACCTGC 3 cut(s) 1225, 1706, 1939
Cac8I GCNNGC 8 cut(s) 328, 417, 643, 654, 873, 1026, 1443, 1512
CaiI CAGNNNCTG 2 cut(s) 992, 1105
CfoI GCGC 6 cut(s) 224, 450, 755, 825, 968, 1621
Cfr10I RCCGGY 3 cut(s) 41, 326, 1853
Cfr13I GGNCC 2 cut(s) 481, 1861
CseI GACGC 2 cut(s) 864, 1388
Csp6I GTAC 8 cut(s) 706, 761, 1257, 1303, 1333, 1372, 1576, 1952
CviQI GTAC 8 cut(s) 706, 761, 1257, 1303, 1333, 1372, 1576, 1952
DdeI CTNAG 3 cut(s) 1725, 1940, 1983
DpnI GATC 7 cut(s) 285, 512, 622, 1059, 1683, 1938, 1945
DpnII GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
DraI TTTAAA 1 cut(s) 52
DraIII CACNNNGTG 1 cut(s) 551
DriI GACNNNNNGTC 1 cut(s) 475
EaeI YGGCCR 2 cut(s) 39, 328
Eam1104I CTCTTC 6 cut(s) 393, 440, 592, 825, 891, 1621
Eam1105I GACNNNNNGTC 1 cut(s) 475
EarI CTCTTC 6 cut(s) 393, 440, 592, 825, 891, 1621
Eco130I CCWWGG 2 cut(s) 105, 1182
Eco24I GRGCYC 1 cut(s) 1400
Eco31I GGTCTC 1 cut(s) 1882
Eco47I GGWCC 1 cut(s) 1861
Eco47III AGCGCT 1 cut(s) 754
Eco57I CTGAAG 2 cut(s) 1050, 1127
EcoRII CCWGG 1 cut(s) 1053
EcoT14I CCWWGG 2 cut(s) 105, 1182
EcoT22I ATGCAT 2 cut(s) 1011, 1531
EcoT38I GRGCYC 1 cut(s) 1400
ErhI CCWWGG 2 cut(s) 105, 1182
Esp3I CGTCTC 1 cut(s) 243
FalI AAGNNNNNCTT 6 cut(s) 72, 104, 75, 107, 1100, 1132
FbaI TGATCA 1 cut(s) 510
FokI GGATG 5 cut(s) 122, 1142, 1327, 1469, 1516
FriOI GRGCYC 1 cut(s) 1400
FspBI CTAG 2 cut(s) 96, 1761
GlaI GCGC 6 cut(s) 223, 449, 754, 824, 967, 1620
HaeII RGCGCY 3 cut(s) 225, 756, 969
HaeIII GGCC 6 cut(s) 41, 330, 336, 404, 482, 519
HapII CCGG 5 cut(s) 42, 219, 273, 327, 1854
HgaI GACGC 2 cut(s) 864, 1388
HhaI GCGC 6 cut(s) 224, 450, 755, 825, 968, 1621
Hin1I GRCGYC 2 cut(s) 252, 1380
Hin6I GCGC 6 cut(s) 222, 448, 753, 823, 966, 1619
HinP1I GCGC 6 cut(s) 222, 448, 753, 823, 966, 1619
HincII GTYRAC 2 cut(s) 1084, 1423
HindII GTYRAC 2 cut(s) 1084, 1423
HindIII AAGCTT 2 cut(s) 117, 1114
HinfI GANTC 7 cut(s) 166, 182, 743, 1142, 1352, 1723, 1730
HpaII CCGG 5 cut(s) 42, 219, 273, 327, 1854
HphI GGTGA 4 cut(s) 114, 615, 1370, 1474
Hpy166II GTNNAC 3 cut(s) 1084, 1305, 1423
Hpy188I TCNGA 7 cut(s) 248, 257, 302, 665, 1107, 1686, 1941
Hpy188III TCNNGA 6 cut(s) 273, 651, 1139, 1496, 1727, 1864
Hpy8I GTNNAC 3 cut(s) 1084, 1305, 1423
Hpy99I CGWCG 2 cut(s) 257, 830
HpyAV CCTTC 3 cut(s) 884, 1393, 1657
HpyCH4III ACNGT 4 cut(s) 126, 145, 1101, 1179
HpyCH4IV ACGT 6 cut(s) 252, 693, 1255, 1887, 1954, 1959
HpyF10VI GCNNNNNNNGC 8 cut(s) 421, 430, 642, 915, 1132, 1163, 1217, 1283
HpyF3I CTNAG 3 cut(s) 1725, 1940, 1983
HpySE526I ACGT 6 cut(s) 252, 693, 1255, 1887, 1954, 1959
Hsp92I GRCGYC 2 cut(s) 252, 1380
HspAI GCGC 6 cut(s) 222, 448, 753, 823, 966, 1619
Kpn2I TCCGGA 1 cut(s) 272
KroI GCCGGC 1 cut(s) 326
KroNI GCCGGC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
LguI GCTCTTC 1 cut(s) 440
LmnI GCTCC 5 cut(s) 567, 739, 939, 1403, 1551
LweI GCATC 6 cut(s) 100, 170, 996, 1349, 1538, 1683
MaeI CTAG 2 cut(s) 96, 1761
MaeII ACGT 6 cut(s) 252, 693, 1255, 1887, 1954, 1959
MaeIII GTNAC 4 cut(s) 44, 1346, 1412, 1955
MalI GATC 7 cut(s) 285, 512, 622, 1059, 1683, 1938, 1945
MboI GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 1 cut(s) 1936
MhlI GDGCHC 2 cut(s) 556, 1400
MluCI AATT 8 cut(s) 22, 304, 682, 738, 1270, 1279, 1777, 1971
MlyI GAGTC 4 cut(s) 160, 191, 1361, 1717
MmeI TCCRAC 3 cut(s) 235, 297, 378
Mph1103I ATGCAT 2 cut(s) 1011, 1531
MroI TCCGGA 1 cut(s) 272
MroNI GCCGGC 1 cut(s) 326
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 2 cut(s) 51, 387
MspA1I CMGCKG 2 cut(s) 1028, 1208
MspI CCGG 5 cut(s) 42, 219, 273, 327, 1854
MspR9I CCNGG 2 cut(s) 219, 1055
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 1 cut(s) 988
MvaI CCWGG 1 cut(s) 1055
MvnI CGCG 3 cut(s) 652, 825, 1571
MwoI GCNNNNNNNGC 8 cut(s) 421, 430, 642, 915, 1132, 1163, 1217, 1283
NaeI GCCGGC 1 cut(s) 328
NciI CCSGG 1 cut(s) 219
NcoI CCATGG 1 cut(s) 105
NdeII GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
NgoMIV GCCGGC 1 cut(s) 326
NlaIV GGNNCC 2 cut(s) 563, 1399
NmeAIII GCCGAG 1 cut(s) 612
NmuCI GTSAC 3 cut(s) 44, 1346, 1955
NruI TCGCGA 1 cut(s) 652
NsiI ATGCAT 2 cut(s) 1011, 1531
NspI RCATGY 3 cut(s) 419, 922, 1546
NspV TTCGAA 1 cut(s) 796
PaeI GCATGC 1 cut(s) 419
PaqCI CACCTGC 1 cut(s) 1225
PciSI GCTCTTC 1 cut(s) 440
PctI GAATGC 1 cut(s) 988
PdiI GCCGGC 1 cut(s) 328
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 3 cut(s) 743, 1142, 1730
PfoI TCCNGGA 1 cut(s) 1053
PleI GAGTC 4 cut(s) 160, 190, 1360, 1717
PpsI GAGTC 4 cut(s) 160, 190, 1360, 1717
Ppu21I YACGTR 1 cut(s) 694
PshBI ATTAAT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 1053
PspGI CCWGG 1 cut(s) 1053
PspN4I GGNNCC 2 cut(s) 563, 1399
PspPI GGNCC 2 cut(s) 481, 1861
PsrI GAACNNNNNNTAC 2 cut(s) 1892, 1924
PstI CTGCAG 1 cut(s) 1717
PstNI CAGNNNCTG 2 cut(s) 992, 1105
PsuI RGATCY 1 cut(s) 1936
PvuII CAGCTG 2 cut(s) 1028, 1208
RruI TCGCGA 1 cut(s) 652
RsaI GTAC 8 cut(s) 707, 762, 1258, 1304, 1334, 1373, 1577, 1953
RsaNI GTAC 8 cut(s) 706, 761, 1257, 1303, 1333, 1372, 1576, 1952
SapI GCTCTTC 1 cut(s) 440
SaqAI TTAA 2 cut(s) 51, 387
Sau3AI GATC 7 cut(s) 283, 510, 620, 1057, 1681, 1936, 1943
Sau96I GGNCC 2 cut(s) 481, 1861
ScaI AGTACT 2 cut(s) 762, 1577
SchI GAGTC 4 cut(s) 160, 191, 1361, 1717
ScrFI CCNGG 2 cut(s) 219, 1055
SduI GDGCHC 2 cut(s) 556, 1400
SfaNI GCATC 6 cut(s) 100, 170, 996, 1349, 1538, 1683
SfcI CTRYAG 3 cut(s) 525, 1579, 1713
SfuI TTCGAA 1 cut(s) 796
SinI GGWCC 1 cut(s) 1861
SmlI CTYRAG 3 cut(s) 428, 756, 1903
SmoI CTYRAG 3 cut(s) 428, 756, 1903
SphI GCATGC 1 cut(s) 419
Sse9I AATT 8 cut(s) 22, 304, 682, 738, 1270, 1279, 1777, 1971
SsiI CCGC 3 cut(s) 1212, 1432, 1571
SspI AATATT 2 cut(s) 382, 1647
SspMI CTAG 2 cut(s) 96, 1761
StyD4I CCNGG 2 cut(s) 217, 1053
StyI CCWWGG 2 cut(s) 105, 1182
TaaI ACNGT 4 cut(s) 126, 145, 1101, 1179
TaiI ACGT 6 cut(s) 255, 696, 1258, 1890, 1957, 1962
TaqI TCGA 5 cut(s) 164, 796, 1326, 1650, 1839
TasI AATT 8 cut(s) 22, 304, 682, 738, 1270, 1279, 1777, 1971
TatI WGTACW 3 cut(s) 760, 1302, 1575
TauI GCSGC 1 cut(s) 1214
TfiI GAWTC 3 cut(s) 743, 1142, 1730
Tru1I TTAA 2 cut(s) 51, 387
Tru9I TTAA 2 cut(s) 51, 387
TscAI CASTG 3 cut(s) 142, 606, 993
TseFI GTSAC 3 cut(s) 44, 1346, 1955
Tsp45I GTSAC 3 cut(s) 44, 1346, 1955
TspDTI ATGAA 4 cut(s) 800, 1108, 1442, 1928
TspGWI ACGGA 1 cut(s) 1086
TspRI CASTG 3 cut(s) 142, 606, 993
VpaK11BI GGWCC 1 cut(s) 1861
VspI ATTAAT 1 cut(s) 387
XapI RAATTY 3 cut(s) 22, 304, 1971
XceI RCATGY 3 cut(s) 419, 922, 1546
XcmI CCANNNNNNNNNTGG 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 2 cut(s) 96, 1761
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 2 cut(s) 762, 1577
Zsp2I ATGCAT 2 cut(s) 1011, 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.