Rw2G023720

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011011
Physical Location & Seq
Reverse (-)
34759874 .. 34763839
3966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G023720.1

Sequence Viewer

Length: 1959 bp
ATGGAGGCCGAAGCTTCACCGTTTGAGAGCAGTGAGTTGGTGGCGAGTTTCCTGGCGTCGACGCCTTTGCTAAGCGAGTCATGGAGGCTGTGCAGCGTCGCCAACGCTTCCGCTCCATTGGGTTTCGTATCGGAGCGAATCGGAGATGTCGGGTACTTTGCTTTCTCGGCCTGCAGTACCCAAATGATCACGGAGGGGTCGGAATACTACTCCACTTGCAGAAACTTGGTGCCATTGGATATCGCAGGCGCCGGCGCCGGTGACGGGCTTTTTTCGGTATTGACCAAAAGTAGTAACAGTAGTATCAATGGAGAAGAAGATGAGGGTGCAGTGATGGTTCATGCAGGGCTGCTCAATCTCTTCTTGTTTCTTCGTAGCTCCCCAGCTTTCCAAGACCAGATGTTGGCGCTGATAAATGGAAGCAAGTCCATTGTGATCACAGGCCATTCTATAGGAGGAACAACTGCCTCACTCTGCGCTCTTTGGATCCTTTGCTACCTGCAATCCGTCTCTTCTACCCTTTCAGTGCTTTGCATTACCTTCGGTTCACCATTACTTGGGAACGAATCGCTTTCCCGAGCAATCCTTAGGGAAAGATGGGGTGGCAATTTTTGCCATGTGGTGTCCAAGTATGACATCATGCCAAGACTCCTATTCGCTCCTTTAGAGTCCTGCACTCGCCAACTTCATTTCTTGCTGCAATACTGGCAGATGTCTATGTCGGCTTCTTCTCCAGGCAACTTTGGACAGTCTCCAGTATTTCAACTCGGTGAGGAAGAGAAAGCTCAGCTCTTCAGCTTCATATCACATTATAATTTGCCAGTGTCTTCAGTAGCACAAGCAGAAGCAATAGAAGAGAAAGCAAATAGTTTGTATTGGCCATTTGGAAACTACGTGTTTTGCTCACAAGAGGGAGCTATTTGTGTGGAGAATGCAGCATCTGTGATCGAGATGATGCATTTGATGTTTATGACTGGTAATCCCAGTTGCTGTATCGATGATCATCTAAAATACGGACACTATGTTGGGAAGATTTCTTCACAGTTTCTGAATAAGAAAAGCTTCTTGCAAGGGGAAGTGATTCCTGAGTCAAGCTATGAAGCAGGGGTTGAACTGGCATTGCAGTCATCAGGAATAAGTAAACAGGAACCAGCTGCCAGACCGGCGGAAGATTGCCTAAAGAAGGCTAGGGGAATGAGACATATGCGTACACCAAGCCTCAACGCTGCAAACTTAGCCATCAGATTATCCAAGGTTGTACCTTACAGAGCTCAAATAGAGTGGTATATGGCATCCTGTGATAAGTCTGATGAGCAACTAGGTTACTACGACTCCTTCAAGCAACGGGGATCATCCAAAAGGGACCACAAAGTCAACATGAACCGGCTCTTGCTTGCTGGATTTTGGAATGATGTGCTCCGCATGCTGGAGAACAACGAGCTGCCTCATGATTTTCACAAGCGATCAAAATGGGTAAACACTTCCCACTTTTACAAGCTCCTGGTAGAGCCACTGGACATTGCAGAGTATTATAGATCTGGTATGCACCGGATCAAGGGTCATTACTTGAAGCATGGAAGAGAGAGGAGGTTTGAGATTTTCGACAGGTGGTGGGGACAGAGACAGGTTGGTCCAGAAGAAAACACTACTAGTACGAGAAGCAAATTTGCAGGTTTGACTCAGGACTCTTGCTTCTGGGCTAAGGTGGAGGAAGCTACAGAGTGGCTAGCTAATGTTAGAAGCGAGAGTGATGCAAGGAAGCTGGCTCTGCTGTGGGAAAATATCAATAACTTTGAAAGGTATGCAACCAGATTAGTTGATTGCAAGGAGGTCTCTAAAGACGTTGTAGCAAAGAATTCGAGCTATTCTAAGTGGGTAGAAGAGTTGAGAGAATTGAAATCTCAGATGCAGCAAATCCATCCTCAGTTCCCAAGGTTTAATGGTGAGTTATTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

652

Amino Acids

73.33

Weight (kDa)

6.22

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 105 - 219 2.5e-16 Lipase (class 3)
EDS1_EP PF18117 424 - 635 1e-71 Enhanced disease susceptibility 1 protein EP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52430
fragaria_vesca FvH4_1g24940 FvH4_1g24940 FvH4_1g24940
malus_domestica MD15G1136300.v1.1
prunus_persica Prupe.4G276500_v2.0.a1 Prupe.4G276500_v2.0.a1
pyrus_communis pycom15g12290
rosa_chinensis RchiOBHm_Chr2g0122591
rosa_laevigata RLG00000018693
rosa_multiflora Rmu_sc0003133.1_g000001 Rmu_sc0010907.1_g000001
rosa_roxburghii Rroxscaffold_2G00121450
rosa_rugosa Rorug02G0239700 Rorug02G0239800
rosa_samantha Rh2AG298400 Rh2BG307100 Rh2CG285200 Rh2DG322000
rosa_wichuraiana Rw2G023720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 813
AasI GACNNNNNNGTC 2 cut(s) 1370, 1629
Acc36I ACCTGC 2 cut(s) 507, 1661
AccB1I GGYRCC 3 cut(s) 229, 248, 254
AccB7I CCANNNNNTGG 2 cut(s) 403, 557
AccBSI CCGCTC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 59
AciI CCGC 3 cut(s) 111, 1166, 1420
AclWI GGATC 4 cut(s) 481, 494, 1357, 1559
AcoI YGGCCR 1 cut(s) 878
AcsI RAATTY 2 cut(s) 1664, 1855
AcuI CTGAAG 2 cut(s) 778, 813
AcyI GRCGYC 4 cut(s) 56, 62, 249, 255
AfaI GTAC 5 cut(s) 155, 178, 1210, 1260, 1654
AfiI CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1183, 1426, 1555
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 6 cut(s) 764, 1112, 1339, 1570, 1796, 1897
AhlI ACTAGT 1 cut(s) 1649
AjnI CCWGG 3 cut(s) 51, 733, 1500
Alw21I GWGCWC 2 cut(s) 1273, 1419
Alw26I GTCTC 5 cut(s) 514, 756, 1192, 1615, 1837
AlwI GGATC 4 cut(s) 481, 494, 1357, 1559
AlwNI CAGNNNCTG 3 cut(s) 941, 990, 1048
Ama87I CYCGRG 1 cut(s) 576
AoxI GGCC 4 cut(s) 6, 168, 442, 878
ApeKI GCWGC 8 cut(s) 93, 349, 697, 935, 1154, 1226, 1441, 1909
ApoI RAATTY 2 cut(s) 1664, 1855
Asp700I GAANNNNTTC 2 cut(s) 1061, 1080
AspLEI GCGC 4 cut(s) 251, 257, 409, 479
AspS9I GGNCC 2 cut(s) 1363, 1631
AsuHPI GGTGA 5 cut(s) 9, 272, 540, 782, 1955
AsuNHI GCTAGC 1 cut(s) 1726
AvaI CYCGRG 1 cut(s) 576
AvaII GGWCC 2 cut(s) 1363, 1631
AxyI CCTNAGG 1 cut(s) 587
BaeI ACNNNNGTAYC 2 cut(s) 286, 319
BalI TGGCCA 1 cut(s) 880
BamHI GGATCC 1 cut(s) 486
BanI GGYRCC 3 cut(s) 229, 248, 254
BanII GRGCYC 1 cut(s) 1273
BbsI GAAGAC 1 cut(s) 819
Bbv12I GWGCWC 2 cut(s) 1273, 1419
BbvI GCAGC 8 cut(s) 105, 336, 684, 947, 1141, 1213, 1428, 1921
BccI CCATC 4 cut(s) 328, 591, 1247, 1926
BcgI CGANNNNNNTGC 8 cut(s) 49, 83, 523, 557, 1733, 1767, 1839, 1873
BciT130I CCWGG 3 cut(s) 53, 735, 1502
BclI TGATCA 3 cut(s) 186, 435, 1000
BcoDI GTCTC 5 cut(s) 514, 756, 1192, 1615, 1837
BcuI ACTAGT 1 cut(s) 1649
BfaI CTAG 4 cut(s) 1188, 1319, 1650, 1727
BfmI CTRYAG 3 cut(s) 172, 450, 1716
BfoI RGCGCY 3 cut(s) 252, 258, 410
BfuAI ACCTGC 2 cut(s) 507, 1661
BglI GCCNNNNNGGC 1 cut(s) 1163
BglII AGATCT 1 cut(s) 1535
BisI GCNGC 8 cut(s) 94, 350, 698, 936, 1155, 1227, 1442, 1910
BlpI GCTNAGC 2 cut(s) 71, 786
BlsI GCNGC 8 cut(s) 95, 351, 699, 937, 1156, 1228, 1443, 1911
Bme1390I CCNGG 3 cut(s) 53, 735, 1502
Bme18I GGWCC 2 cut(s) 1363, 1631
BmeT110I CYCGRG 1 cut(s) 576
BmgT120I GGNCC 2 cut(s) 1363, 1631
BmiI GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
BmrFI CCNGG 3 cut(s) 53, 735, 1502
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 5 cut(s) 945, 947, 1301, 1741, 1896
BmtI GCTAGC 1 cut(s) 1730
BmuI ACTGGG 1 cut(s) 978
BpiI GAAGAC 1 cut(s) 819
BpmI CTGGAG 3 cut(s) 717, 738, 1448
Bpu10I CCTNAGC 1 cut(s) 1701
Bpu1102I GCTNAGC 2 cut(s) 71, 786
Bsa29I ATCGAT 1 cut(s) 996
BsaAI YACGTR 1 cut(s) 895
BsaBI GATNNNNATC 1 cut(s) 1002
BsaHI GRCGYC 4 cut(s) 56, 62, 249, 255
BsaI GGTCTC 1 cut(s) 1837
BsaJI CCNNGG 2 cut(s) 1251, 1931
BsaWI WCCGGW 1 cut(s) 1548
BsaXI ACNNNNNCTCC 2 cut(s) 1316, 1346
Bsc4I CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1183, 1426, 1555
Bse118I RCCGGY 4 cut(s) 251, 257, 1162, 1383
Bse1I ACTGG 7 cut(s) 710, 755, 821, 979, 984, 1119, 1518
Bse21I CCTNAGG 1 cut(s) 587
Bse3DI GCAATG 2 cut(s) 1118, 1518
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 3 cut(s) 53, 735, 1502
BseCI ATCGAT 1 cut(s) 996
BseDI CCNNGG 2 cut(s) 1251, 1931
BseGI GGATG 3 cut(s) 1292, 1352, 1918
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1183, 1426, 1555
BseMI GCAATG 2 cut(s) 1118, 1518
BseMII CTCAG 5 cut(s) 800, 1077, 1694, 1916, 1937
BseNI ACTGG 7 cut(s) 710, 755, 821, 979, 984, 1119, 1518
BseRI GAGGAG 1 cut(s) 1600
BseXI GCAGC 8 cut(s) 105, 336, 684, 947, 1141, 1213, 1428, 1921
BseYI CCCAGC 1 cut(s) 382
BsgI GTGCAG 3 cut(s) 112, 348, 658
BshFI GGCC 4 cut(s) 8, 170, 444, 880
BshNI GGYRCC 3 cut(s) 229, 248, 254
BshVI ATCGAT 1 cut(s) 996
BsiHKAI GWGCWC 2 cut(s) 1273, 1419
BsiHKCI CYCGRG 1 cut(s) 576
BsiSI CCGG 5 cut(s) 252, 258, 1163, 1384, 1549
BslFI GGGAC 2 cut(s) 1376, 1629
BslI CCNNNNNNNGG 6 cut(s) 264, 403, 557, 1183, 1426, 1555
BsmAI GTCTC 5 cut(s) 514, 756, 1192, 1615, 1837
BsmBI CGTCTC 1 cut(s) 514
BsmFI GGGAC 2 cut(s) 1376, 1629
BsmI GAATGC 1 cut(s) 937
BsnI GGCC 4 cut(s) 8, 170, 444, 880
Bso31I GGTCTC 1 cut(s) 1837
BsoBI CYCGRG 1 cut(s) 576
Bsp1286I GDGCHC 2 cut(s) 1273, 1419
Bsp143I GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
Bsp1720I GCTNAGC 2 cut(s) 71, 786
BspACI CCGC 3 cut(s) 111, 1166, 1420
BspANI GGCC 4 cut(s) 8, 170, 444, 880
BspCNI CTCAG 5 cut(s) 799, 1078, 1693, 1915, 1936
BspDI ATCGAT 1 cut(s) 996
BspHI TCATGA 1 cut(s) 1447
BspLI GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
BspMAI CTGCAG 1 cut(s) 176
BspMI ACCTGC 2 cut(s) 507, 1661
BspOI GCTAGC 1 cut(s) 1730
BspPI GGATC 4 cut(s) 481, 494, 1357, 1559
BspQI GCTCTTC 1 cut(s) 797
BspT107I GGYRCC 3 cut(s) 229, 248, 254
BspTNI GGTCTC 1 cut(s) 1837
BsrBI CCGCTC 1 cut(s) 113
BsrDI GCAATG 2 cut(s) 1118, 1518
BsrFI RCCGGY 4 cut(s) 251, 257, 1162, 1383
BsrI ACTGG 7 cut(s) 710, 755, 821, 979, 984, 1119, 1518
BssAI RCCGGY 4 cut(s) 251, 257, 1162, 1383
BssECI CCNNGG 2 cut(s) 1251, 1931
BssMI GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
BssNI GRCGYC 4 cut(s) 56, 62, 249, 255
BssT1I CCWWGG 2 cut(s) 1251, 1931
Bst2UI CCWGG 3 cut(s) 53, 735, 1502
Bst4CI ACNGT 4 cut(s) 21, 299, 750, 1044
Bst6I CTCTTC 7 cut(s) 365, 517, 771, 797, 849, 1573, 1875
BstACI GRCGYC 4 cut(s) 56, 62, 249, 255
BstAPI GCANNNNNTGC 1 cut(s) 612
BstBAI YACGTR 1 cut(s) 895
BstC8I GCNNGC 7 cut(s) 172, 247, 253, 1395, 1424, 1728, 1764
BstENI CCTNNNNNAGG 1 cut(s) 1181
BstF5I GGATG 3 cut(s) 1292, 1352, 1918
BstH2I RGCGCY 3 cut(s) 252, 258, 410
BstHHI GCGC 4 cut(s) 251, 257, 409, 479
BstKTI GATC 9 cut(s) 189, 438, 489, 948, 1003, 1352, 1466, 1538, 1554
BstMAI GTCTC 5 cut(s) 514, 756, 1192, 1615, 1837
BstMBI GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
BstMWI GCNNNNNNNGC 7 cut(s) 167, 612, 706, 1163, 1235, 1423, 1768
BstNI CCWGG 3 cut(s) 53, 735, 1502
BstNSI RCATGY 1 cut(s) 1426
BstSCI CCNGG 3 cut(s) 51, 733, 1500
BstSFI CTRYAG 3 cut(s) 172, 450, 1716
BstV1I GCAGC 8 cut(s) 105, 336, 684, 947, 1141, 1213, 1428, 1921
BstV2I GAAGAC 1 cut(s) 819
BstX2I RGATCY 2 cut(s) 486, 1535
BstYI RGATCY 2 cut(s) 486, 1535
Bsu15I ATCGAT 1 cut(s) 996
Bsu36I CCTNAGG 1 cut(s) 587
BsuRI GGCC 4 cut(s) 8, 170, 444, 880
BsuTUI ATCGAT 1 cut(s) 996
BtsCI GGATG 3 cut(s) 1292, 1352, 1918
BtsI GCAGTG 2 cut(s) 37, 336
BtsIMutI CAGTG 5 cut(s) 37, 336, 531, 828, 1511
BveI ACCTGC 2 cut(s) 507, 1661
Cac8I GCNNGC 7 cut(s) 172, 247, 253, 1395, 1424, 1728, 1764
CaiI CAGNNNCTG 3 cut(s) 941, 990, 1048
CciI TCATGA 1 cut(s) 1447
CfoI GCGC 4 cut(s) 251, 257, 409, 479
Cfr10I RCCGGY 4 cut(s) 251, 257, 1162, 1383
Cfr13I GGNCC 2 cut(s) 1363, 1631
ClaI ATCGAT 1 cut(s) 996
CseI GACGC 3 cut(s) 45, 70, 85
Csp6I GTAC 5 cut(s) 154, 177, 1209, 1259, 1653
CspCI CAANNNNNGTGG 2 cut(s) 1262, 1297
CviAII CATG 8 cut(s) 81, 341, 617, 640, 1378, 1423, 1448, 1574
CviQI GTAC 5 cut(s) 154, 177, 1209, 1259, 1653
DinI GGCGCC 2 cut(s) 250, 256
DpnI GATC 9 cut(s) 188, 437, 488, 947, 1002, 1351, 1465, 1537, 1553
DpnII GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
DrdI GACNNNNNNGTC 2 cut(s) 1370, 1629
DseDI GACNNNNNNGTC 2 cut(s) 1370, 1629
EaeI YGGCCR 1 cut(s) 878
Eam1104I CTCTTC 7 cut(s) 365, 517, 771, 797, 849, 1573, 1875
EarI CTCTTC 7 cut(s) 365, 517, 771, 797, 849, 1573, 1875
EciI GGCGGA 1 cut(s) 1181
Ecl136II GAGCTC 1 cut(s) 1271
Eco130I CCWWGG 2 cut(s) 1251, 1931
Eco24I GRGCYC 1 cut(s) 1273
Eco31I GGTCTC 1 cut(s) 1837
Eco32I GATATC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 1363, 1631
Eco53kI GAGCTC 1 cut(s) 1271
Eco57I CTGAAG 2 cut(s) 778, 813
Eco81I CCTNAGG 1 cut(s) 587
Eco88I CYCGRG 1 cut(s) 576
EcoICRI GAGCTC 1 cut(s) 1271
EcoNI CCTNNNNNAGG 1 cut(s) 1181
EcoRI GAATTC 1 cut(s) 1855
EcoRII CCWGG 3 cut(s) 51, 733, 1500
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 2 cut(s) 1251, 1931
EcoT22I ATGCAT 1 cut(s) 960
EcoT38I GRGCYC 1 cut(s) 1273
EgeI GGCGCC 2 cut(s) 250, 256
EheI GGCGCC 2 cut(s) 250, 256
ErhI CCWWGG 2 cut(s) 1251, 1931
Esp3I CGTCTC 1 cut(s) 514
FaeI CATG 8 cut(s) 84, 344, 620, 643, 1381, 1426, 1451, 1577
FalI AAGNNNNNCTT 2 cut(s) 1046, 1078
FaqI GGGAC 2 cut(s) 1376, 1629
FatI CATG 8 cut(s) 80, 340, 616, 639, 1377, 1422, 1447, 1573
FauNDI CATATG 1 cut(s) 1203
FbaI TGATCA 3 cut(s) 186, 435, 1000
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 8 cut(s) 94, 350, 698, 936, 1155, 1227, 1442, 1910
FokI GGATG 3 cut(s) 1279, 1339, 1905
FriOI GRGCYC 1 cut(s) 1273
Fsp4HI GCNGC 8 cut(s) 94, 350, 698, 936, 1155, 1227, 1442, 1910
FspBI CTAG 4 cut(s) 1188, 1319, 1650, 1727
GlaI GCGC 4 cut(s) 250, 256, 408, 478
GluI GCNGC 8 cut(s) 94, 350, 698, 936, 1155, 1227, 1442, 1910
GsaI CCCAGC 1 cut(s) 386
GsuI CTGGAG 3 cut(s) 717, 738, 1448
HaeII RGCGCY 3 cut(s) 252, 258, 410
HaeIII GGCC 4 cut(s) 8, 170, 444, 880
HapII CCGG 5 cut(s) 252, 258, 1163, 1384, 1549
HgaI GACGC 3 cut(s) 45, 70, 85
HhaI GCGC 4 cut(s) 251, 257, 409, 479
Hin1I GRCGYC 4 cut(s) 56, 62, 249, 255
Hin1II CATG 8 cut(s) 84, 344, 620, 643, 1381, 1426, 1451, 1577
Hin6I GCGC 4 cut(s) 249, 255, 407, 477
HinP1I GCGC 4 cut(s) 249, 255, 407, 477
HincII GTYRAC 2 cut(s) 60, 1375
HindII GTYRAC 2 cut(s) 60, 1375
HindIII AAGCTT 2 cut(s) 12, 1060
HpaII CCGG 5 cut(s) 252, 258, 1163, 1384, 1549
HphI GGTGA 5 cut(s) 9, 272, 540, 782, 1955
Hpy166II GTNNAC 6 cut(s) 60, 548, 1142, 1211, 1375, 1477
Hpy188I TCNGA 7 cut(s) 133, 143, 202, 1050, 1244, 1309, 1905
Hpy188III TCNNGA 7 cut(s) 576, 949, 1085, 1131, 1448, 1634, 1682
Hpy8I GTNNAC 6 cut(s) 60, 548, 1142, 1211, 1375, 1477
Hpy99I CGWCG 3 cut(s) 61, 64, 101
HpyAV CCTTC 3 cut(s) 550, 1177, 1345
HpyCH4III ACNGT 4 cut(s) 21, 299, 750, 1044
HpyCH4IV ACGT 2 cut(s) 894, 1842
HpyF10VI GCNNNNNNNGC 7 cut(s) 167, 612, 706, 1163, 1235, 1423, 1768
HpySE526I ACGT 2 cut(s) 894, 1842
Hsp92I GRCGYC 4 cut(s) 56, 62, 249, 255
Hsp92II CATG 8 cut(s) 84, 344, 620, 643, 1381, 1426, 1451, 1577
HspAI GCGC 4 cut(s) 249, 255, 407, 477
KasI GGCGCC 2 cut(s) 248, 254
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
Ksp22I TGATCA 3 cut(s) 186, 435, 1000
Kzo9I GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
LguI GCTCTTC 1 cut(s) 797
LmnI GCTCC 7 cut(s) 118, 133, 383, 664, 914, 1422, 1503
Lsp1109I GCAGC 8 cut(s) 105, 336, 684, 947, 1141, 1213, 1428, 1921
LweI GCATC 5 cut(s) 945, 947, 1301, 1741, 1896
MaeI CTAG 4 cut(s) 1188, 1319, 1650, 1727
MaeII ACGT 2 cut(s) 894, 1842
MaeIII GTNAC 3 cut(s) 260, 293, 1322
MalI GATC 9 cut(s) 188, 437, 488, 947, 1002, 1351, 1465, 1537, 1553
MbiI CCGCTC 1 cut(s) 113
MboI GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
MflI RGATCY 2 cut(s) 486, 1535
MhlI GDGCHC 2 cut(s) 1273, 1419
MlsI TGGCCA 1 cut(s) 880
MluCI AATT 5 cut(s) 607, 814, 1664, 1855, 1892
MluNI TGGCCA 1 cut(s) 880
Mly113I GGCGCC 2 cut(s) 249, 255
MlyI GAGTC 7 cut(s) 86, 642, 677, 1097, 1325, 1672, 1679
MmeI TCCRAC 1 cut(s) 180
Mox20I TGGCCA 1 cut(s) 880
Mph1103I ATGCAT 1 cut(s) 960
MreI CGCCGGCG 1 cut(s) 251
MroNI GCCGGC 1 cut(s) 251
MroXI GAANNNNTTC 2 cut(s) 1061, 1080
MscI TGGCCA 1 cut(s) 880
MseI TTAA 1 cut(s) 1938
Msp20I TGGCCA 1 cut(s) 880
MspA1I CMGCKG 1 cut(s) 1154
MspI CCGG 5 cut(s) 252, 258, 1163, 1384, 1549
MspR9I CCNGG 3 cut(s) 53, 735, 1502
Mva1269I GAATGC 1 cut(s) 937
MvaI CCWGG 3 cut(s) 53, 735, 1502
MwoI GCNNNNNNNGC 7 cut(s) 167, 612, 706, 1163, 1235, 1423, 1768
NaeI GCCGGC 1 cut(s) 253
NarI GGCGCC 2 cut(s) 249, 255
NdeI CATATG 1 cut(s) 1203
NdeII GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
NgoMIV GCCGGC 1 cut(s) 251
NheI GCTAGC 1 cut(s) 1726
NlaIII CATG 8 cut(s) 84, 344, 620, 643, 1381, 1426, 1451, 1577
NlaIV GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 1 cut(s) 260
NsiI ATGCAT 1 cut(s) 960
NspI RCATGY 1 cut(s) 1426
PaeI GCATGC 1 cut(s) 1426
PagI TCATGA 1 cut(s) 1447
PciSI GCTCTTC 1 cut(s) 797
PctI GAATGC 1 cut(s) 937
PdiI GCCGGC 1 cut(s) 253
PdmI GAANNNNTTC 2 cut(s) 1061, 1080
PfeI GAWTC 3 cut(s) 138, 566, 1081
PflMI CCANNNNNTGG 2 cut(s) 403, 557
PkrI GCNGC 8 cut(s) 95, 351, 699, 937, 1156, 1228, 1443, 1911
PleI GAGTC 7 cut(s) 85, 642, 676, 1096, 1325, 1672, 1679
PluTI GGCGCC 2 cut(s) 252, 258
PpsI GAGTC 7 cut(s) 85, 642, 676, 1096, 1325, 1672, 1679
Ppu21I YACGTR 1 cut(s) 895
PsiI TTATAA 1 cut(s) 813
Psp124BI GAGCTC 1 cut(s) 1273
Psp6I CCWGG 3 cut(s) 51, 733, 1500
PspFI CCCAGC 1 cut(s) 382
PspGI CCWGG 3 cut(s) 51, 733, 1500
PspN4I GGNNCC 6 cut(s) 231, 250, 256, 488, 1149, 1364
PspPI GGNCC 2 cut(s) 1363, 1631
PstI CTGCAG 1 cut(s) 176
PstNI CAGNNNCTG 3 cut(s) 941, 990, 1048
PsuI RGATCY 2 cut(s) 486, 1535
PvuII CAGCTG 1 cut(s) 1154
RsaI GTAC 5 cut(s) 155, 178, 1210, 1260, 1654
RsaNI GTAC 5 cut(s) 154, 177, 1209, 1259, 1653
SacI GAGCTC 1 cut(s) 1273
SalI GTCGAC 1 cut(s) 58
SapI GCTCTTC 1 cut(s) 797
SaqAI TTAA 1 cut(s) 1938
SatI GCNGC 8 cut(s) 94, 350, 698, 936, 1155, 1227, 1442, 1910
Sau3AI GATC 9 cut(s) 186, 435, 486, 945, 1000, 1349, 1463, 1535, 1551
Sau96I GGNCC 2 cut(s) 1363, 1631
SchI GAGTC 7 cut(s) 86, 642, 677, 1097, 1325, 1672, 1679
ScrFI CCNGG 3 cut(s) 53, 735, 1502
SduI GDGCHC 2 cut(s) 1273, 1419
SfaNI GCATC 5 cut(s) 945, 947, 1301, 1741, 1896
SfcI CTRYAG 3 cut(s) 172, 450, 1716
SfoI GGCGCC 2 cut(s) 250, 256
SgrAI CRCCGGYG 2 cut(s) 251, 257
SgrDI CGTCGACG 1 cut(s) 58
SinI GGWCC 2 cut(s) 1363, 1631
SpeI ACTAGT 1 cut(s) 1649
SphI GCATGC 1 cut(s) 1426
Sse9I AATT 5 cut(s) 607, 814, 1664, 1855, 1892
SsiI CCGC 3 cut(s) 111, 1166, 1420
SspDI GGCGCC 2 cut(s) 248, 254
SspMI CTAG 4 cut(s) 1188, 1319, 1650, 1727
SstI GAGCTC 1 cut(s) 1273
StyD4I CCNGG 3 cut(s) 51, 733, 1500
StyI CCWWGG 2 cut(s) 1251, 1931
TaaI ACNGT 4 cut(s) 21, 299, 750, 1044
TaiI ACGT 2 cut(s) 897, 1845
TaqI TCGA 5 cut(s) 59, 948, 996, 1602, 1859
TasI AATT 5 cut(s) 607, 814, 1664, 1855, 1892
TfiI GAWTC 3 cut(s) 138, 566, 1081
Tru1I TTAA 1 cut(s) 1938
Tru9I TTAA 1 cut(s) 1938
TscAI CASTG 5 cut(s) 37, 336, 531, 828, 1518
TseFI GTSAC 1 cut(s) 260
TseI GCWGC 8 cut(s) 93, 349, 697, 935, 1154, 1226, 1441, 1909
Tsp45I GTSAC 1 cut(s) 260
TspDTI ATGAA 5 cut(s) 329, 677, 790, 1113, 1394
TspGWI ACGGA 3 cut(s) 206, 496, 1029
TspRI CASTG 5 cut(s) 37, 336, 531, 828, 1518
Van91I CCANNNNNTGG 2 cut(s) 403, 557
VpaK11BI GGWCC 2 cut(s) 1363, 1631
XagI CCTNNNNNAGG 1 cut(s) 1181
XapI RAATTY 2 cut(s) 1664, 1855
XceI RCATGY 1 cut(s) 1426
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 2 cut(s) 1061, 1080
XspI CTAG 4 cut(s) 1188, 1319, 1650, 1727
Zsp2I ATGCAT 1 cut(s) 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.