AT3G53100

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006535
Physical Location & Seq
Reverse (-)
19684686 .. 19686984
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G53100.1

Sequence Viewer

Length: 1056 bp
ATGCAAAAGATGAGAGTTTCGGGGTTTAGAGTTCTTCTCTTGGTTTCTTGTTTCTTCTGCAAATCCAAAGGAGCTGTTGTTCCTGCACTCATCATGTTTGGCGACTCCATTGTTGACGTTGGCAACAACAATAACTTACTCAGCATTGTCAAATCCAACTTTCTTCCTTACGGTAGAGACTTCATCGACCAAAGACCCACCGGAAGATTCTGCAACGGAAAACTCGCTGTAGATTTCTCAGCCGAGTACCTTGGTTTCTCTTCATACCCACCTGCATTCCTAAGCCGGGAAGCAAGCAACGAGAATATCCTGATCGGTGCCAACTTTGCATCAGCTTCTTCAGGATACTATGATGCCACATCTGTTCCATTTGGCTCGATCTCCTTAACACGACAGCTGAGCTATTACAGGGCGTATCAAAATAGAGTAACGAGAATGATCGGGAGGGGGAACGCGCGCATACTGTTCTCTAGAGGTATCCACATTTTGAGTGCGGGATCTAGCGACTTTCTTCAGAATTATTACATCAATCCTCTACTCAACATCCTAAATACACCTGATCAGTTCGCTGACATTCTCCTGAGATCCTTCTCAGAATTCATCCAGAATCTGTATGAACTAGGAGCAAGGAGAATCGGAGTGATATCGCTACCGCCGATGGGTTGCCTGCCTGCAGCTATCACTCTTTTCGGTGCAGGAAACAAAAGTTGTGTTGAGAGACTGAACAATGATGCTATCATGTTCAATACTAAACTTGAGAACACAACCCGGTTACTGATGAATAGACACTCAGGCCTCAGACTCGTCGCCTTTAATGTCTACCAACCTTTCCTGGACATCATCACCAACCCAACTGACAATGGATTCTTTGAAACAAAAAGGGCATGCTGCGGAACAGGGACAATAGAAACATCGTTCCTTTGCAATTCTCTATCCTTCGGAACGTGTGTAAATGCCACAGGATATGTGTTTTGGGATGGATTTCATCCCACAGAGGCTGTAAACGAGCTGTTGGCTGGTCAACTACTGGGTCAAGGGATTTCTCTAATCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

38.69

Weight (kDa)

8.7

Isoelectric Point (pI)

30.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 339 1.1e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 280
Acc36I ACCTGC 1 cut(s) 280
AccB1I GGYRCC 1 cut(s) 317
AccI GTMKAC 1 cut(s) 819
AccII CGCG 2 cut(s) 455, 457
AciI CCGC 3 cut(s) 494, 653, 891
AclWI GGATC 2 cut(s) 505, 579
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 2 cut(s) 324, 497
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 2 cut(s) 286, 659
AflIII ACRYGT 1 cut(s) 944
AgsI TTSAA 2 cut(s) 745, 872
AjnI CCWGG 1 cut(s) 831
AloI GAACNNNNNNTCC 2 cut(s) 63, 95
AluBI AGCT 6 cut(s) 74, 335, 397, 402, 677, 1009
AluI AGCT 6 cut(s) 74, 335, 397, 402, 677, 1009
Alw26I GTCTC 2 cut(s) 171, 712
AlwI GGATC 2 cut(s) 505, 579
AlwNI CAGNNNCTG 2 cut(s) 610, 998
AoxI GGCC 1 cut(s) 793
ApeKI GCWGC 2 cut(s) 674, 888
ApoI RAATTY 1 cut(s) 596
AspLEI GCGC 2 cut(s) 457, 459
AsuC2I CCSGG 2 cut(s) 287, 769
AsuHPI GGTGA 1 cut(s) 835
BanI GGYRCC 1 cut(s) 317
BbvI GCAGC 2 cut(s) 686, 875
BccI CCATC 2 cut(s) 652, 971
BciT130I CCWGG 1 cut(s) 833
BciVI GTATCC 2 cut(s) 338, 488
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 2 cut(s) 287, 769
BcoDI GTCTC 2 cut(s) 171, 712
BfaI CTAG 3 cut(s) 471, 501, 620
BfmI CTRYAG 2 cut(s) 228, 672
BfuAI ACCTGC 1 cut(s) 280
BfuI GTATCC 2 cut(s) 338, 488
BisI GCNGC 2 cut(s) 675, 889
BlpI GCTNAGC 1 cut(s) 398
BlsI GCNGC 2 cut(s) 676, 890
Bme1390I CCNGG 3 cut(s) 287, 769, 833
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 3 cut(s) 287, 769, 833
BmrI ACTGGG 1 cut(s) 1037
BmsI GCATC 3 cut(s) 338, 343, 721
BmuI ACTGGG 1 cut(s) 1037
BplI GAGNNNNNCTC 2 cut(s) 21, 53
Bpu10I CCTNAGC 1 cut(s) 281
Bpu1102I GCTNAGC 1 cut(s) 398
BpuEI CTTGAG 1 cut(s) 776
BpuMI CCSGG 2 cut(s) 287, 769
BsaJI CCNNGG 1 cut(s) 250
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 659
Bse1I ACTGG 1 cut(s) 1032
BseBI CCWGG 1 cut(s) 833
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 4 cut(s) 543, 600, 982, 985
BseLI CCNNNNNNNGG 2 cut(s) 286, 659
BseMII CTCAG 7 cut(s) 154, 252, 389, 572, 606, 804, 811
BseNI ACTGG 1 cut(s) 1032
BsePI GCGCGC 1 cut(s) 455
BseXI GCAGC 2 cut(s) 686, 875
BsgI GTGCAG 2 cut(s) 69, 714
Bsh1236I CGCG 2 cut(s) 455, 457
BshFI GGCC 1 cut(s) 795
BshNI GGYRCC 1 cut(s) 317
BsiSI CCGG 3 cut(s) 201, 286, 769
BslFI GGGAC 1 cut(s) 913
BslI CCNNNNNNNGG 2 cut(s) 286, 659
BsmAI GTCTC 2 cut(s) 171, 712
BsmFI GGGAC 1 cut(s) 913
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 795
Bsp143I GATC 6 cut(s) 312, 378, 438, 497, 559, 584
Bsp1720I GCTNAGC 1 cut(s) 398
BspACI CCGC 3 cut(s) 494, 653, 891
BspANI GGCC 1 cut(s) 795
BspCNI CTCAG 7 cut(s) 153, 251, 390, 573, 605, 803, 810
BspFNI CGCG 2 cut(s) 455, 457
BspLI GGNNCC 1 cut(s) 319
BspMAI CTGCAG 1 cut(s) 676
BspMI ACCTGC 1 cut(s) 280
BspPI GGATC 2 cut(s) 505, 579
BspT107I GGYRCC 1 cut(s) 317
BsrI ACTGG 1 cut(s) 1032
BssECI CCNNGG 1 cut(s) 250
BssHII GCGCGC 1 cut(s) 455
BssMI GATC 6 cut(s) 312, 378, 438, 497, 559, 584
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 2 cut(s) 173, 465
Bst6I CTCTTC 1 cut(s) 265
BstC8I GCNNGC 5 cut(s) 295, 457, 668, 672, 886
BstDEI CTNAG 8 cut(s) 140, 238, 281, 398, 581, 592, 790, 797
BstF5I GGATG 4 cut(s) 543, 600, 982, 985
BstFNI CGCG 2 cut(s) 455, 457
BstHHI GCGC 2 cut(s) 457, 459
BstKTI GATC 6 cut(s) 315, 381, 441, 500, 562, 587
BstMAI GTCTC 2 cut(s) 171, 712
BstMBI GATC 6 cut(s) 312, 378, 438, 497, 559, 584
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNI CCWGG 1 cut(s) 833
BstNSI RCATGY 1 cut(s) 888
BstSCI CCNGG 3 cut(s) 285, 767, 831
BstSFI CTRYAG 2 cut(s) 228, 672
BstUI CGCG 2 cut(s) 455, 457
BstV1I GCAGC 2 cut(s) 686, 875
BstX2I RGATCY 2 cut(s) 497, 584
BstYI RGATCY 2 cut(s) 497, 584
BsuI GTATCC 2 cut(s) 338, 488
BsuRI GGCC 1 cut(s) 795
BtsCI GGATG 4 cut(s) 543, 600, 982, 985
BveI ACCTGC 1 cut(s) 280
Cac8I GCNNGC 5 cut(s) 295, 457, 668, 672, 886
CaiI CAGNNNCTG 2 cut(s) 610, 998
CfoI GCGC 2 cut(s) 457, 459
Csp6I GTAC 1 cut(s) 247
CviAII CATG 3 cut(s) 94, 739, 885
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 8 cut(s) 140, 238, 281, 398, 581, 592, 790, 797
DpnI GATC 6 cut(s) 314, 380, 440, 499, 561, 586
DpnII GATC 6 cut(s) 312, 378, 438, 497, 559, 584
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 250
Eco147I AGGCCT 1 cut(s) 795
Eco32I GATATC 1 cut(s) 645
Eco57I CTGAAG 2 cut(s) 324, 497
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 1 cut(s) 831
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 3 cut(s) 97, 742, 888
FaiI YATR 8 cut(s) 95, 265, 351, 461, 615, 740, 886, 966
FaqI GGGAC 1 cut(s) 913
FatI CATG 3 cut(s) 93, 738, 884
FauI CCCGC 1 cut(s) 487
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 1 cut(s) 819
Fnu4HI GCNGC 2 cut(s) 675, 889
FokI GGATG 4 cut(s) 530, 587, 972, 989
Fsp4HI GCNGC 2 cut(s) 675, 889
FspBI CTAG 3 cut(s) 471, 501, 620
GlaI GCGC 2 cut(s) 456, 458
GluI GCNGC 2 cut(s) 675, 889
HaeIII GGCC 1 cut(s) 795
HapII CCGG 3 cut(s) 201, 286, 769
HhaI GCGC 2 cut(s) 457, 459
Hin1II CATG 3 cut(s) 97, 742, 888
Hin6I GCGC 2 cut(s) 455, 457
HinP1I GCGC 2 cut(s) 455, 457
HincII GTYRAC 2 cut(s) 115, 1022
HindII GTYRAC 2 cut(s) 115, 1022
HinfI GANTC 6 cut(s) 104, 207, 607, 633, 801, 864
HpaII CCGG 3 cut(s) 201, 286, 769
HphI GGTGA 1 cut(s) 835
Hpy166II GTNNAC 4 cut(s) 115, 820, 1003, 1022
Hpy188I TCNGA 5 cut(s) 516, 595, 638, 800, 941
Hpy188III TCNNGA 6 cut(s) 310, 342, 442, 471, 580, 604
Hpy8I GTNNAC 4 cut(s) 115, 820, 1003, 1022
Hpy99I CGWCG 1 cut(s) 809
HpyAV CCTTC 2 cut(s) 598, 946
HpyCH4III ACNGT 2 cut(s) 173, 465
HpyCH4IV ACGT 2 cut(s) 117, 944
HpyCH4V TGCA 9 cut(s) 4, 60, 86, 213, 275, 329, 674, 695, 924
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 8 cut(s) 140, 238, 281, 398, 581, 592, 790, 797
HpySE526I ACGT 2 cut(s) 117, 944
Hsp92II CATG 3 cut(s) 97, 742, 888
HspAI GCGC 2 cut(s) 455, 457
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 6 cut(s) 312, 378, 438, 497, 559, 584
LmnI GCTCC 2 cut(s) 71, 623
Lsp1109I GCAGC 2 cut(s) 686, 875
LweI GCATC 3 cut(s) 338, 343, 721
MaeI CTAG 3 cut(s) 471, 501, 620
MaeII ACGT 2 cut(s) 117, 944
MaeIII GTNAC 2 cut(s) 427, 771
MalI GATC 6 cut(s) 314, 380, 440, 499, 561, 586
MboI GATC 6 cut(s) 312, 378, 438, 497, 559, 584
MboII GAAGA 7 cut(s) 26, 46, 155, 216, 252, 330, 503
MfeI CAATTG 1 cut(s) 1051
MflI RGATCY 2 cut(s) 497, 584
MluCI AATT 4 cut(s) 517, 596, 925, 1051
MlyI GAGTC 2 cut(s) 98, 795
MmeI TCCRAC 1 cut(s) 180
MnlI CCTC 5 cut(s) 438, 467, 543, 806, 988
MseI TTAA 2 cut(s) 386, 813
MspA1I CMGCKG 1 cut(s) 397
MspI CCGG 3 cut(s) 201, 286, 769
MspR9I CCNGG 3 cut(s) 287, 769, 833
MunI CAATTG 1 cut(s) 1051
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 1 cut(s) 833
MvnI CGCG 2 cut(s) 455, 457
MwoI GCNNNNNNNGC 1 cut(s) 326
NciI CCSGG 2 cut(s) 287, 769
NdeII GATC 6 cut(s) 312, 378, 438, 497, 559, 584
NlaIII CATG 3 cut(s) 97, 742, 888
NlaIV GGNNCC 1 cut(s) 319
NmeAIII GCCGAG 1 cut(s) 268
NspI RCATGY 1 cut(s) 888
PaeI GCATGC 1 cut(s) 888
PaqCI CACCTGC 1 cut(s) 280
PauI GCGCGC 1 cut(s) 455
PceI AGGCCT 1 cut(s) 795
PcsI WCGNNNNNNNCGW 1 cut(s) 653
PctI GAATGC 1 cut(s) 275
PfeI GAWTC 4 cut(s) 207, 607, 633, 864
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 2 cut(s) 676, 890
PleI GAGTC 2 cut(s) 98, 795
PpsI GAGTC 2 cut(s) 98, 795
Psp6I CCWGG 1 cut(s) 831
PspGI CCWGG 1 cut(s) 831
PspN4I GGNNCC 1 cut(s) 319
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 2 cut(s) 610, 998
PsuI RGATCY 2 cut(s) 497, 584
PteI GCGCGC 1 cut(s) 455
PvuII CAGCTG 1 cut(s) 397
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
SaqAI TTAA 2 cut(s) 386, 813
SatI GCNGC 2 cut(s) 675, 889
Sau3AI GATC 6 cut(s) 312, 378, 438, 497, 559, 584
SchI GAGTC 2 cut(s) 98, 795
ScrFI CCNGG 3 cut(s) 287, 769, 833
SfaNI GCATC 3 cut(s) 338, 343, 721
SfcI CTRYAG 2 cut(s) 228, 672
SmlI CTYRAG 1 cut(s) 755
SmoI CTYRAG 1 cut(s) 755
SphI GCATGC 1 cut(s) 888
Sse9I AATT 4 cut(s) 517, 596, 925, 1051
SseBI AGGCCT 1 cut(s) 795
SsiI CCGC 3 cut(s) 494, 653, 891
SspMI CTAG 3 cut(s) 471, 501, 620
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 3 cut(s) 285, 767, 831
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 2 cut(s) 173, 465
TaiI ACGT 2 cut(s) 120, 947
TaqI TCGA 2 cut(s) 186, 377
TasI AATT 4 cut(s) 517, 596, 925, 1051
TfiI GAWTC 4 cut(s) 207, 607, 633, 864
Tru1I TTAA 2 cut(s) 386, 813
Tru9I TTAA 2 cut(s) 386, 813
TseI GCWGC 2 cut(s) 674, 888
TspDTI ATGAA 6 cut(s) 172, 252, 589, 630, 794, 974
TspGWI ACGGA 1 cut(s) 231
XapI RAATTY 1 cut(s) 596
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 1 cut(s) 888
XmiI GTMKAC 1 cut(s) 819
XspI CTAG 3 cut(s) 471, 501, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.