Rroxscaffold_6G00409830

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0006535
Physical Location & Seq
Reverse (-)
32336686 .. 32338406
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409830.1

Sequence Viewer

Length: 921 bp
ATGATAGTTTCCGGTGAACTCTTGGTTTCCTTGAGCCTTCTGATTCTTGTTTTCAAAGTGTCTCATGGGCAGCCTTTAGTTCCTGCACTCATCATCTTTGGGGACTCTGTTGCTGATGTGGGAAATAATAACAACCGCACTACACTAGTGAAGGCAAACTTCCCTCCTTATGGAAGAGACTTCGTTAATCATCAACCGACTGGAAGATTTTGTAATGGGAAGCTTGCCACAGACTTCACCGGGTGCAATTCACTAGACCAACAATTAAATTACTACAATGAGTATCAAAGTATAGTGGTGAATATGGTAGGGGAAGCCGAAGCCAATGCCATATTCTCTGGCGCCATCCACCTTCTAAGTGCAGGAACCAGCGATTTCATTCAGAACTACTACATCAATCCCTTTCGTAGAGTCTACACACCTGACCAATTCTCAGAAATTCTAATGAGATCCTACTCCTCTTTCATTCAGAAGTTGTATGGACTCGGAGCACGAAGGATTGGAGTCACAAACTTACCACCTATAGGATGTTTGCCAGCAGCAATTACCCTATTTGGCTCAGGAAGCAACCAGTGGAACATAAGATTGAATCAAGATGCTATTTTATTCAATAACAAGCTTAACAACACATCTCAAACTTTGCAGAAGAGTCTTCCCGGACTCAAGCTCGTAGTTTTTTATATCTGCCAGCCCCTCCTGGATATGATCACAAATCCCAGTGACAATGGGTTCTTCGAGTCAAGAAGAGCTTGTTGTGGAACAGGTACATTTGAAACATCTGTGCTCTGCAACTCTAGGTCTGTAGGAACATGCAACAATGCAACTGGTTATGTGTTCTGGGATGGATTTCATCCCTCTGAAGCTGCCAATGAGGTCTTAGCTGGTGATCTACTTGCACAGGGCATCGATCTCATATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

306

Amino Acids

33.48

Weight (kDa)

5.79

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 294 1.9e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 341
AccI GTMKAC 1 cut(s) 414
AciI CCGC 1 cut(s) 136
AclWI GGATC 1 cut(s) 444
AcsI RAATTY 1 cut(s) 438
AcuI CTGAAG 1 cut(s) 879
AcyI GRCGYC 1 cut(s) 342
AdeI CACNNNGTG 1 cut(s) 243
AfaI GTAC 1 cut(s) 766
AfiI CCNNNNNNNGG 2 cut(s) 170, 524
AgsI TTSAA 4 cut(s) 55, 589, 610, 773
AhlI ACTAGT 1 cut(s) 145
AjnI CCWGG 1 cut(s) 696
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 6 cut(s) 223, 619, 667, 749, 863, 881
AluI AGCT 6 cut(s) 223, 619, 667, 749, 863, 881
Alw21I GWGCWC 2 cut(s) 493, 786
Alw26I GTCTC 2 cut(s) 66, 171
AlwI GGATC 1 cut(s) 444
ApeKI GCWGC 3 cut(s) 70, 539, 863
ApoI RAATTY 1 cut(s) 438
AspLEI GCGC 1 cut(s) 344
AsuC2I CCSGG 2 cut(s) 241, 657
AsuHPI GGTGA 4 cut(s) 26, 229, 310, 896
BanI GGYRCC 1 cut(s) 341
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 2 cut(s) 493, 786
BbvI GCAGC 3 cut(s) 82, 551, 850
BccI CCATC 2 cut(s) 353, 836
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 698
BclI TGATCA 1 cut(s) 705
BcnI CCSGG 2 cut(s) 241, 657
BcoDI GTCTC 2 cut(s) 66, 171
BcuI ACTAGT 1 cut(s) 145
BfaI CTAG 3 cut(s) 146, 254, 795
BfmI CTRYAG 2 cut(s) 522, 801
BfoI RGCGCY 1 cut(s) 345
BisI GCNGC 3 cut(s) 71, 540, 864
BlsI GCNGC 3 cut(s) 72, 541, 865
Bme1390I CCNGG 3 cut(s) 241, 657, 698
BmiI GGNNCC 2 cut(s) 343, 367
BmrFI CCNGG 3 cut(s) 241, 657, 698
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 2 cut(s) 586, 912
BmuI ACTGGG 1 cut(s) 711
BpiI GAAGAC 1 cut(s) 644
Bpu10I CCTNAGC 1 cut(s) 559
BpuEI CTTGAG 2 cut(s) 52, 647
BpuMI CCSGG 2 cut(s) 241, 657
Bsa29I ATCGAT 1 cut(s) 906
BsaHI GRCGYC 1 cut(s) 342
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 524
Bse1I ACTGG 4 cut(s) 205, 571, 717, 829
BseBI CCWGG 1 cut(s) 698
BseCI ATCGAT 1 cut(s) 906
BseGI GGATG 4 cut(s) 345, 533, 847, 850
BseLI CCNNNNNNNGG 2 cut(s) 170, 524
BseMII CTCAG 2 cut(s) 447, 573
BseNI ACTGG 4 cut(s) 205, 571, 717, 829
BseRI GAGGAG 1 cut(s) 448
BseXI GCAGC 3 cut(s) 82, 551, 850
BsgI GTGCAG 2 cut(s) 69, 381
BshNI GGYRCC 1 cut(s) 341
BshVI ATCGAT 1 cut(s) 906
BsiHKAI GWGCWC 2 cut(s) 493, 786
BsiSI CCGG 3 cut(s) 12, 240, 657
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 170, 524
BsmAI GTCTC 2 cut(s) 66, 171
BsmFI GGGAC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 493, 786
Bsp143I GATC 4 cut(s) 449, 705, 886, 907
BspACI CCGC 1 cut(s) 136
BspCNI CTCAG 2 cut(s) 446, 572
BspDI ATCGAT 1 cut(s) 906
BspLI GGNNCC 2 cut(s) 343, 367
BspPI GGATC 1 cut(s) 444
BspQI GCTCTTC 1 cut(s) 739
BspT107I GGYRCC 1 cut(s) 341
BsrI ACTGG 4 cut(s) 205, 571, 717, 829
BssMI GATC 4 cut(s) 449, 705, 886, 907
BssNI GRCGYC 1 cut(s) 342
Bst2UI CCWGG 1 cut(s) 698
Bst6I CTCTTC 3 cut(s) 169, 641, 739
BstACI GRCGYC 1 cut(s) 342
BstC8I GCNNGC 3 cut(s) 225, 537, 689
BstDEI CTNAG 4 cut(s) 356, 433, 559, 877
BstF5I GGATG 4 cut(s) 345, 533, 847, 850
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 4 cut(s) 452, 708, 889, 910
BstMAI GTCTC 2 cut(s) 66, 171
BstMBI GATC 4 cut(s) 449, 705, 886, 907
BstMWI GCNNNNNNNGC 1 cut(s) 564
BstNI CCWGG 1 cut(s) 698
BstNSI RCATGY 1 cut(s) 813
BstSCI CCNGG 3 cut(s) 239, 655, 696
BstSFI CTRYAG 2 cut(s) 522, 801
BstV1I GCAGC 3 cut(s) 82, 551, 850
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 1 cut(s) 449
BstYI RGATCY 1 cut(s) 449
Bsu15I ATCGAT 1 cut(s) 906
BsuTUI ATCGAT 1 cut(s) 906
BtsCI GGATG 4 cut(s) 345, 533, 847, 850
BtsIMutI CAGTG 2 cut(s) 578, 724
Cac8I GCNNGC 3 cut(s) 225, 537, 689
CfoI GCGC 1 cut(s) 344
ClaI ATCGAT 1 cut(s) 906
Csp6I GTAC 1 cut(s) 765
CviAII CATG 2 cut(s) 65, 810
CviQI GTAC 1 cut(s) 765
DdeI CTNAG 4 cut(s) 356, 433, 559, 877
DinI GGCGCC 1 cut(s) 343
DpnI GATC 4 cut(s) 451, 707, 888, 909
DpnII GATC 4 cut(s) 449, 705, 886, 907
DraIII CACNNNGTG 1 cut(s) 243
Eam1104I CTCTTC 3 cut(s) 169, 641, 739
EarI CTCTTC 3 cut(s) 169, 641, 739
Eco57I CTGAAG 1 cut(s) 879
EcoRII CCWGG 1 cut(s) 696
EgeI GGCGCC 1 cut(s) 343
EheI GGCGCC 1 cut(s) 343
FaeI CATG 2 cut(s) 68, 813
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 733, 765
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 64, 809
FbaI TGATCA 1 cut(s) 705
FblI GTMKAC 1 cut(s) 414
Fnu4HI GCNGC 3 cut(s) 71, 540, 864
FokI GGATG 4 cut(s) 332, 540, 837, 854
Fsp4HI GCNGC 3 cut(s) 71, 540, 864
FspBI CTAG 3 cut(s) 146, 254, 795
GlaI GCGC 1 cut(s) 343
GluI GCNGC 3 cut(s) 71, 540, 864
HaeII RGCGCY 1 cut(s) 345
HapII CCGG 3 cut(s) 12, 240, 657
HhaI GCGC 1 cut(s) 344
Hin1I GRCGYC 1 cut(s) 342
Hin1II CATG 2 cut(s) 68, 813
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HindIII AAGCTT 2 cut(s) 221, 617
HinfI GANTC 9 cut(s) 43, 104, 411, 483, 504, 589, 649, 660, 737
HpaII CCGG 3 cut(s) 12, 240, 657
HphI GGTGA 4 cut(s) 26, 229, 310, 896
Hpy166II GTNNAC 2 cut(s) 17, 415
Hpy188I TCNGA 6 cut(s) 42, 384, 436, 471, 488, 859
Hpy188III TCNNGA 4 cut(s) 561, 593, 741, 918
Hpy8I GTNNAC 2 cut(s) 17, 415
HpyAV CCTTC 4 cut(s) 47, 145, 362, 489
HpyCH4V TGCA 8 cut(s) 86, 246, 362, 643, 789, 813, 821, 896
HpyF10VI GCNNNNNNNGC 1 cut(s) 564
HpyF3I CTNAG 4 cut(s) 356, 433, 559, 877
Hsp92I GRCGYC 1 cut(s) 342
Hsp92II CATG 2 cut(s) 68, 813
HspAI GCGC 1 cut(s) 342
KasI GGCGCC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 705
Kzo9I GATC 4 cut(s) 449, 705, 886, 907
LguI GCTCTTC 1 cut(s) 739
LmnI GCTCC 1 cut(s) 488
Lsp1109I GCAGC 3 cut(s) 82, 551, 850
LweI GCATC 2 cut(s) 586, 912
MaeI CTAG 3 cut(s) 146, 254, 795
MaeIII GTNAC 2 cut(s) 505, 719
MalI GATC 4 cut(s) 451, 707, 888, 909
MboI GATC 4 cut(s) 449, 705, 886, 907
MboII GAAGA 6 cut(s) 186, 216, 644, 658, 724, 756
MflI RGATCY 1 cut(s) 449
MhlI GDGCHC 2 cut(s) 493, 786
MluCI AATT 6 cut(s) 247, 263, 268, 428, 438, 543
Mly113I GGCGCC 1 cut(s) 342
MlyI GAGTC 7 cut(s) 98, 420, 477, 513, 654, 658, 746
MnlI CCTC 5 cut(s) 174, 469, 704, 865, 865
MseI TTAA 3 cut(s) 186, 266, 621
MspI CCGG 3 cut(s) 12, 240, 657
MspR9I CCNGG 3 cut(s) 241, 657, 698
MvaI CCWGG 1 cut(s) 698
MwoI GCNNNNNNNGC 1 cut(s) 564
NarI GGCGCC 1 cut(s) 342
NciI CCSGG 2 cut(s) 241, 657
NdeII GATC 4 cut(s) 449, 705, 886, 907
NlaIII CATG 2 cut(s) 68, 813
NlaIV GGNNCC 2 cut(s) 343, 367
NmuCI GTSAC 2 cut(s) 505, 719
NspI RCATGY 1 cut(s) 813
PciSI GCTCTTC 1 cut(s) 739
PfeI GAWTC 2 cut(s) 43, 589
PfoI TCCNGGA 2 cut(s) 655, 696
PkrI GCNGC 3 cut(s) 72, 541, 865
PleI GAGTC 7 cut(s) 98, 419, 477, 512, 654, 657, 745
PluTI GGCGCC 1 cut(s) 345
PpsI GAGTC 7 cut(s) 98, 419, 477, 512, 654, 657, 745
Psp6I CCWGG 1 cut(s) 696
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 2 cut(s) 343, 367
PsuI RGATCY 1 cut(s) 449
RsaI GTAC 1 cut(s) 766
RsaNI GTAC 1 cut(s) 765
SapI GCTCTTC 1 cut(s) 739
SaqAI TTAA 3 cut(s) 186, 266, 621
SatI GCNGC 3 cut(s) 71, 540, 864
Sau3AI GATC 4 cut(s) 449, 705, 886, 907
SchI GAGTC 7 cut(s) 98, 420, 477, 513, 654, 658, 746
ScrFI CCNGG 3 cut(s) 241, 657, 698
SduI GDGCHC 2 cut(s) 493, 786
SfaNI GCATC 2 cut(s) 586, 912
SfcI CTRYAG 2 cut(s) 522, 801
SfoI GGCGCC 1 cut(s) 343
SmlI CTYRAG 2 cut(s) 31, 662
SmoI CTYRAG 2 cut(s) 31, 662
SpeI ACTAGT 1 cut(s) 145
Sse9I AATT 6 cut(s) 247, 263, 268, 428, 438, 543
SsiI CCGC 1 cut(s) 136
SspDI GGCGCC 1 cut(s) 341
SspMI CTAG 3 cut(s) 146, 254, 795
StyD4I CCNGG 3 cut(s) 239, 655, 696
TaqI TCGA 2 cut(s) 735, 906
TasI AATT 6 cut(s) 247, 263, 268, 428, 438, 543
TfiI GAWTC 2 cut(s) 43, 589
Tru1I TTAA 3 cut(s) 186, 266, 621
Tru9I TTAA 3 cut(s) 186, 266, 621
TscAI CASTG 2 cut(s) 578, 724
TseFI GTSAC 2 cut(s) 505, 719
TseI GCWGC 3 cut(s) 70, 539, 863
Tsp45I GTSAC 2 cut(s) 505, 719
TspDTI ATGAA 3 cut(s) 367, 454, 839
TspRI CASTG 2 cut(s) 578, 724
XapI RAATTY 1 cut(s) 438
XceI RCATGY 1 cut(s) 813
XmiI GTMKAC 1 cut(s) 414
XspI CTAG 3 cut(s) 146, 254, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.