Rw3G015850

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0006535
Physical Location & Seq
Forward (+)
16382999 .. 16385571
2573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015850.1

Sequence Viewer

Length: 1056 bp
ATGGAGTTTTCAAGTAGTAAACTCTTGGGTTCCTTGAGCTTTCTTGCTCTAGTTTTCACAGTCGTTCTTGGAGACCCTTTAGTTCCAGCTCTTATCATCTTTGGGGACTCTGTAGTCGATGTGGGAAACAATAACAATCTCAACACAGTCATCAAGGCCAACTTTCCTCCTTATGGAAGAGACTTTGTGAATCACAAACCAACTGGAAGATTCTGTAATGGAAAGCTTGCCACCGATTTCACTGCTGAATATCTGGGATTCACTTCATATCCACCAGCTTACCTAAGCCAAGAAGCCAGCGGGAAGAACCTCTTGACTGGAGTCAACTTTGCCTCAGCTGCTTCTGGATTTTATGACCCAACAGCCCAGTTATATAATTCGATTTCACTGACCCAGCAACTGAATAACTACAAGGAGTATCAAAGTAAAGTGGTGAATCTGGTAGGACAGACTAAAGCCAATGCCATATTCTCTGGAGGCATCCACCTTCTAAGTGCAGGGAGCAGTGATTTCATTCAGAACTACTACATCACTCCCCTTGTTACGGTCTATACGCCCGATCAATTCTCAGACATTCTCATTAGATCCTACTCTACATTCATTCAGAATTTGTATGGGTTGGGAGCAAGAAGGATTGGAGTCACAAGCTTACCGCCAACAGGGTGTTTGCCAGCAGCAATCACCCTATTTGGCAATGGAAATAACCAATGCGTCACAAGACTAAACGGAGATGCCATGTCATTCAACAAGAAGCTAAAGAGCACATCTCAAAGTTTGCAGACCAAACTTCCGGGCCTCAAGCTTGTGGTCTTCGATATCTTCCAGCCGCTGCTGGATATGGTCACAAAGCCTAGTGATAATGGTTTCATGGAGTCGAGACGAGCTTGCTGTGGAACAGGAACATTAGAAACCTCTGTGCTTTGCAACTCGGGGTCTATAGGAACATGTAGCAATGTGACTGGTTATGTGTTTTGGGATGGATTCCATCCCTCTCAAGCTGCCAACGAGGTCTTGGCCGGTGATCTACTTCAGCAGGGTTTCGATCTTATATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

37.8

Weight (kDa)

6.29

Isoelectric Point (pI)

27.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 339 4e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AciI CCGC 3 cut(s) 300, 653, 827
AclWI GGATC 1 cut(s) 579
AcoI YGGCCR 1 cut(s) 1014
AcsI RAATTY 1 cut(s) 607
AcuI CTGAAG 1 cut(s) 1013
AfiI CCNNNNNNNGG 3 cut(s) 173, 544, 659
AflIII ACRYGT 1 cut(s) 944
AgsI TTSAA 2 cut(s) 12, 745
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
Alw21I GWGCWC 1 cut(s) 764
Alw26I GTCTC 3 cut(s) 66, 174, 871
AlwI GGATC 1 cut(s) 579
AlwNI CAGNNNCTG 2 cut(s) 400, 829
Ama87I CYCGRG 1 cut(s) 928
AoxI GGCC 3 cut(s) 156, 793, 1014
ApeKI GCWGC 4 cut(s) 338, 674, 829, 998
ApoI RAATTY 1 cut(s) 607
AspS9I GGNCC 1 cut(s) 793
AsuC2I CCSGG 1 cut(s) 792
AsuHPI GGTGA 3 cut(s) 445, 673, 1031
AvaI CYCGRG 1 cut(s) 928
BbsI GAAGAC 1 cut(s) 802
Bbv12I GWGCWC 1 cut(s) 764
BbvCI CCTCAGC 1 cut(s) 334
BbvI GCAGC 4 cut(s) 325, 686, 816, 985
BccI CCATC 2 cut(s) 971, 993
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BcnI CCSGG 1 cut(s) 792
BcoDI GTCTC 3 cut(s) 66, 174, 871
BfaI CTAG 2 cut(s) 50, 852
BfmI CTRYAG 2 cut(s) 111, 936
BisI GCNGC 5 cut(s) 339, 675, 827, 830, 999
BlsI GCNGC 5 cut(s) 340, 676, 828, 831, 1000
Bme1390I CCNGG 1 cut(s) 792
BmeT110I CYCGRG 1 cut(s) 928
BmgT120I GGNCC 1 cut(s) 793
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 792
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 2 cut(s) 489, 721
BmuI ACTGGG 1 cut(s) 361
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 802
BplI GAGNNNNNCTC 2 cut(s) 751, 783
BpmI CTGGAG 2 cut(s) 339, 495
Bpu10I CCTNAGC 2 cut(s) 284, 334
BpuEI CTTGAG 3 cut(s) 55, 782, 978
BpuMI CCSGG 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 66
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 544, 659
Bse118I RCCGGY 1 cut(s) 1016
Bse1I ACTGG 4 cut(s) 208, 322, 367, 964
Bse3DI GCAATG 2 cut(s) 700, 958
BseGI GGATG 3 cut(s) 480, 982, 985
BseLI CCNNNNNNNGG 3 cut(s) 173, 544, 659
BseMI GCAATG 2 cut(s) 700, 958
BseMII CTCAG 2 cut(s) 348, 582
BseNI ACTGG 4 cut(s) 208, 322, 367, 964
BseXI GCAGC 4 cut(s) 325, 686, 816, 985
BseYI CCCAGC 1 cut(s) 393
BsgI GTGCAG 1 cut(s) 516
BshFI GGCC 3 cut(s) 158, 795, 1016
BsiHKAI GWGCWC 1 cut(s) 764
BsiHKCI CYCGRG 1 cut(s) 928
BsiSI CCGG 2 cut(s) 791, 1017
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 3 cut(s) 173, 544, 659
BsmAI GTCTC 3 cut(s) 66, 174, 871
BsmBI CGTCTC 1 cut(s) 871
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 3 cut(s) 158, 795, 1016
Bso31I GGTCTC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 928
Bsp1286I GDGCHC 1 cut(s) 764
Bsp143I GATC 4 cut(s) 559, 584, 1021, 1042
BspACI CCGC 3 cut(s) 300, 653, 827
BspANI GGCC 3 cut(s) 158, 795, 1016
BspCNI CTCAG 2 cut(s) 347, 581
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 1 cut(s) 579
BspTNI GGTCTC 1 cut(s) 66
BsrDI GCAATG 2 cut(s) 700, 958
BsrFI RCCGGY 1 cut(s) 1016
BsrI ACTGG 4 cut(s) 208, 322, 367, 964
BssAI RCCGGY 1 cut(s) 1016
BssMI GATC 4 cut(s) 559, 584, 1021, 1042
Bst4CI ACNGT 3 cut(s) 61, 148, 547
Bst6I CTCTTC 1 cut(s) 172
BstC8I GCNNGC 4 cut(s) 228, 298, 672, 886
BstDEI CTNAG 4 cut(s) 284, 334, 491, 568
BstF5I GGATG 3 cut(s) 480, 982, 985
BstKTI GATC 4 cut(s) 562, 587, 1024, 1045
BstMAI GTCTC 3 cut(s) 66, 174, 871
BstMBI GATC 4 cut(s) 559, 584, 1021, 1042
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 948
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 790
BstSFI CTRYAG 2 cut(s) 111, 936
BstV1I GCAGC 4 cut(s) 325, 686, 816, 985
BstV2I GAAGAC 1 cut(s) 802
BstX2I RGATCY 1 cut(s) 584
BstYI RGATCY 1 cut(s) 584
BsuRI GGCC 3 cut(s) 158, 795, 1016
BtsCI GGATG 3 cut(s) 480, 982, 985
BtsI GCAGTG 2 cut(s) 240, 511
BtsIMutI CAGTG 3 cut(s) 240, 386, 511
Cac8I GCNNGC 4 cut(s) 228, 298, 672, 886
CaiI CAGNNNCTG 2 cut(s) 400, 829
Cfr10I RCCGGY 1 cut(s) 1016
Cfr13I GGNCC 1 cut(s) 793
CseI GACGC 1 cut(s) 700
CviAII CATG 3 cut(s) 736, 868, 945
DdeI CTNAG 4 cut(s) 284, 334, 491, 568
DpnI GATC 4 cut(s) 561, 586, 1023, 1044
DpnII GATC 4 cut(s) 559, 584, 1021, 1042
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 1014
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
Eco31I GGTCTC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 817
Eco57I CTGAAG 1 cut(s) 1013
Eco88I CYCGRG 1 cut(s) 928
EcoRV GATATC 1 cut(s) 817
Esp3I CGTCTC 1 cut(s) 871
FaeI CATG 3 cut(s) 739, 871, 948
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 296, 328
FaqI GGGAC 1 cut(s) 119
FatI CATG 3 cut(s) 735, 867, 944
FauI CCCGC 1 cut(s) 293
Fnu4HI GCNGC 5 cut(s) 339, 675, 827, 830, 999
FokI GGATG 3 cut(s) 467, 972, 989
Fsp4HI GCNGC 5 cut(s) 339, 675, 827, 830, 999
FspBI CTAG 2 cut(s) 50, 852
GluI GCNGC 5 cut(s) 339, 675, 827, 830, 999
GsaI CCCAGC 1 cut(s) 397
GsuI CTGGAG 2 cut(s) 339, 495
HaeIII GGCC 3 cut(s) 158, 795, 1016
HapII CCGG 2 cut(s) 791, 1017
HgaI GACGC 1 cut(s) 700
Hin1II CATG 3 cut(s) 739, 871, 948
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 3 cut(s) 224, 646, 800
HinfI GANTC 9 cut(s) 107, 190, 210, 258, 321, 436, 639, 872, 981
HpaII CCGG 2 cut(s) 791, 1017
HphI GGTGA 3 cut(s) 445, 673, 1031
Hpy166II GTNNAC 2 cut(s) 20, 325
Hpy188I TCNGA 3 cut(s) 519, 571, 606
Hpy188III TCNNGA 5 cut(s) 313, 345, 474, 876, 1053
Hpy8I GTNNAC 2 cut(s) 20, 325
HpyAV CCTTC 2 cut(s) 497, 624
HpyCH4III ACNGT 3 cut(s) 61, 148, 547
HpyCH4V TGCA 3 cut(s) 497, 778, 924
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 4 cut(s) 284, 334, 491, 568
Hsp92II CATG 3 cut(s) 739, 871, 948
Kzo9I GATC 4 cut(s) 559, 584, 1021, 1042
LmnI GCTCC 2 cut(s) 501, 623
Lsp1109I GCAGC 4 cut(s) 325, 686, 816, 985
LweI GCATC 2 cut(s) 489, 721
MaeI CTAG 2 cut(s) 50, 852
MaeIII GTNAC 5 cut(s) 541, 640, 712, 841, 955
MalI GATC 4 cut(s) 561, 586, 1023, 1044
MboI GATC 4 cut(s) 559, 584, 1021, 1042
MboII GAAGA 5 cut(s) 189, 219, 316, 802, 811
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 764
MluCI AATT 3 cut(s) 376, 563, 607
MlyI GAGTC 4 cut(s) 101, 330, 648, 881
MnlI CCTC 8 cut(s) 177, 320, 343, 470, 806, 922, 1000, 1000
MspA1I CMGCKG 3 cut(s) 300, 338, 829
MspI CCGG 2 cut(s) 791, 1017
MspR9I CCNGG 1 cut(s) 792
MwoI GCNNNNNNNGC 1 cut(s) 338
NciI CCSGG 1 cut(s) 792
NdeII GATC 4 cut(s) 559, 584, 1021, 1042
NlaIII CATG 3 cut(s) 739, 871, 948
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 4 cut(s) 640, 712, 841, 955
NspI RCATGY 1 cut(s) 948
PciI ACATGT 1 cut(s) 944
PfeI GAWTC 5 cut(s) 190, 210, 258, 436, 981
PkrI GCNGC 5 cut(s) 340, 676, 828, 831, 1000
PleI GAGTC 4 cut(s) 101, 329, 647, 880
PpsI GAGTC 4 cut(s) 101, 329, 647, 880
PscI ACATGT 1 cut(s) 944
PshAI GACNNNNGTC 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 393
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 793
PstNI CAGNNNCTG 2 cut(s) 400, 829
PsuI RGATCY 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 338
SatI GCNGC 5 cut(s) 339, 675, 827, 830, 999
Sau3AI GATC 4 cut(s) 559, 584, 1021, 1042
Sau96I GGNCC 1 cut(s) 793
SchI GAGTC 4 cut(s) 101, 330, 648, 881
ScrFI CCNGG 1 cut(s) 792
SduI GDGCHC 1 cut(s) 764
SfaNI GCATC 2 cut(s) 489, 721
SfcI CTRYAG 2 cut(s) 111, 936
SmlI CTYRAG 3 cut(s) 34, 797, 993
SmoI CTYRAG 3 cut(s) 34, 797, 993
Sse9I AATT 3 cut(s) 376, 563, 607
SsiI CCGC 3 cut(s) 300, 653, 827
SspMI CTAG 2 cut(s) 50, 852
StyD4I CCNGG 1 cut(s) 790
TaaI ACNGT 3 cut(s) 61, 148, 547
TaqI TCGA 5 cut(s) 117, 380, 813, 875, 1041
TasI AATT 3 cut(s) 376, 563, 607
TauI GCSGC 1 cut(s) 829
TfiI GAWTC 5 cut(s) 190, 210, 258, 436, 981
TscAI CASTG 3 cut(s) 247, 393, 511
TseFI GTSAC 4 cut(s) 640, 712, 841, 955
TseI GCWGC 4 cut(s) 338, 674, 829, 998
Tsp45I GTSAC 4 cut(s) 640, 712, 841, 955
TspDTI ATGAA 4 cut(s) 255, 502, 589, 856
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 3 cut(s) 247, 393, 511
XapI RAATTY 1 cut(s) 607
XceI RCATGY 1 cut(s) 948
XcmI CCANNNNNNNNNTGG 1 cut(s) 1009
XspI CTAG 2 cut(s) 50, 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.