RLG00000024157

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006535
Physical Location & Seq
Reverse (-)
32573000 .. 32578968
5969 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024157

Sequence Viewer

Length: 957 bp
ATGATAGTTTCTGGTAAACTCTTGGTTTCCTTGAGCCTTCTAGTTCTTGTTTTCAAAGTGTCTCATGGGCAGCCTTTAGTTCCTGCTGATGTGGGAAATAATAACAACCGCACTAGAGACTTCGTTAATCATCAACCGACTGGAAGATTTTGTAATGGGAAGCTTGCCACAGACTTCACCGCTGAATATCTCGGATTCACCTCATACCCACCAGCTTTCCTTAGCCAAGAAGCCACAAGGATGAGCATTTTGACTGGAGTCAAATTTGCCTCAGCTGCTTCTGGCTATTATAACTGGACAGCCCAGTTATGGGGTGCAATTTCACTAGACCAACAATTAAATTACTACAATGAGTATCAAAGTATAGTGGTGAATATGGTAGGGGAAGCCGAAGCCAATGCCATATTCTCTGGCGCCATCCACCTTCTAAGTGCAGGAACCAGCGATTTCATTTATAACTACTACATCAATCCATTTCTTGGAGTCTACACACCTGACCAATTCTCAGAAATTCTAATGAGATCCTACTCCTCTTTCATTCAGCACGAAGGATTGGAGTCACAAACTTACCACATCCTGCCAGCAGCAATTACCCTATGTGGCTCAGGAAGCAACCAGTGGAACATAAGATTGAATCAAGATGCCATTTTATTCAATAACAAGCTTAACAACACATCTCAAACTACTATTCCCGGACTCAAGCTCGTAGTTTTCGATATCTACCAGCCCCTCCTGGATATGATCACAAATCCCAGTGACAATGGATTCTTCGAGTCAAGAAGAGCTTGTTGTGGAACAGGTACAGTTGAAACATCTGTGCTCTGCAACTCCAGGTCTGTAGGAACATGCAACAATGCAACTGGTTATGTGTTCTGGGATGGATTTCATCCCTCTGAAGCTGCCAATGAGGTCTTACCTGGTGACCTAATTGCTCAGGGCATCGATCTCATACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

319

Amino Acids

34.91

Weight (kDa)

4.93

Isoelectric Point (pI)

30.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 304 5.4e-18 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 291, 456
AccB1I GGYRCC 1 cut(s) 413
AccB7I CCANNNNNTGG 1 cut(s) 479
AccI GTMKAC 1 cut(s) 486
AciI CCGC 2 cut(s) 109, 180
AclWI GGATC 1 cut(s) 516
AcsI RAATTY 2 cut(s) 263, 510
AcuI CTGAAG 1 cut(s) 915
AcyI GRCGYC 1 cut(s) 414
AfaI GTAC 1 cut(s) 802
AfiI CCNNNNNNNGG 3 cut(s) 309, 310, 479
AgsI TTSAA 4 cut(s) 55, 634, 655, 809
AjnI CCWGG 3 cut(s) 732, 830, 916
AjuI GAANNNNNNNTTGG 2 cut(s) 389, 421
AluBI AGCT 7 cut(s) 163, 215, 275, 664, 703, 785, 899
AluI AGCT 7 cut(s) 163, 215, 275, 664, 703, 785, 899
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 2 cut(s) 66, 111
AlwI GGATC 1 cut(s) 516
ApeKI GCWGC 4 cut(s) 70, 275, 584, 899
ApoI RAATTY 2 cut(s) 263, 510
AspLEI GCGC 1 cut(s) 416
AsuC2I CCSGG 1 cut(s) 693
AsuHPI GGTGA 4 cut(s) 169, 190, 382, 932
BanI GGYRCC 1 cut(s) 413
Bbv12I GWGCWC 1 cut(s) 822
BbvCI CCTCAGC 1 cut(s) 271
BbvI GCAGC 4 cut(s) 82, 262, 596, 886
BccI CCATC 2 cut(s) 425, 872
BcgI CGANNNNNNTGC 2 cut(s) 380, 414
BciT130I CCWGG 3 cut(s) 734, 832, 918
BclI TGATCA 1 cut(s) 741
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 2 cut(s) 66, 111
BfaI CTAG 3 cut(s) 41, 114, 326
BfmI CTRYAG 1 cut(s) 837
BfoI RGCGCY 1 cut(s) 417
BisI GCNGC 4 cut(s) 71, 276, 585, 900
BlsI GCNGC 4 cut(s) 72, 277, 586, 901
Bme1390I CCNGG 4 cut(s) 693, 734, 832, 918
BmiI GGNNCC 2 cut(s) 415, 439
BmrFI CCNGG 4 cut(s) 693, 734, 832, 918
BmrI ACTGGG 2 cut(s) 298, 747
BmsI GCATC 2 cut(s) 631, 948
BmuI ACTGGG 2 cut(s) 298, 747
BoxI GACNNNNGTC 1 cut(s) 257
BpmI CTGGAG 2 cut(s) 276, 814
Bpu10I CCTNAGC 4 cut(s) 221, 271, 604, 933
BpuEI CTTGAG 2 cut(s) 52, 683
BpuMI CCSGG 1 cut(s) 693
Bsa29I ATCGAT 1 cut(s) 942
BsaHI GRCGYC 1 cut(s) 414
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 310, 479
Bse1I ACTGG 7 cut(s) 145, 259, 299, 304, 616, 753, 865
BseBI CCWGG 3 cut(s) 734, 832, 918
BseCI ATCGAT 1 cut(s) 942
BseGI GGATG 5 cut(s) 246, 417, 573, 883, 886
BseLI CCNNNNNNNGG 3 cut(s) 309, 310, 479
BseMII CTCAG 4 cut(s) 285, 519, 618, 947
BseNI ACTGG 7 cut(s) 145, 259, 299, 304, 616, 753, 865
BseRI GAGGAG 1 cut(s) 520
BseXI GCAGC 4 cut(s) 82, 262, 596, 886
BsgI GTGCAG 1 cut(s) 453
BshNI GGYRCC 1 cut(s) 413
BshVI ATCGAT 1 cut(s) 942
BsiHKAI GWGCWC 1 cut(s) 822
BsiSI CCGG 1 cut(s) 693
BslI CCNNNNNNNGG 3 cut(s) 309, 310, 479
BsmAI GTCTC 2 cut(s) 66, 111
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 3 cut(s) 521, 741, 943
BspACI CCGC 2 cut(s) 109, 180
BspCNI CTCAG 4 cut(s) 284, 518, 617, 946
BspDI ATCGAT 1 cut(s) 942
BspLI GGNNCC 2 cut(s) 415, 439
BspPI GGATC 1 cut(s) 516
BspQI GCTCTTC 1 cut(s) 775
BspT107I GGYRCC 1 cut(s) 413
BsrI ACTGG 7 cut(s) 145, 259, 299, 304, 616, 753, 865
BssMI GATC 3 cut(s) 521, 741, 943
BssNI GRCGYC 1 cut(s) 414
Bst2UI CCWGG 3 cut(s) 734, 832, 918
Bst4CI ACNGT 1 cut(s) 805
Bst6I CTCTTC 1 cut(s) 775
BstACI GRCGYC 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 165, 582
BstDEI CTNAG 6 cut(s) 221, 271, 428, 505, 604, 933
BstEII GGTNACC 1 cut(s) 920
BstF5I GGATG 5 cut(s) 246, 417, 573, 883, 886
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 3 cut(s) 524, 744, 946
BstMAI GTCTC 2 cut(s) 66, 111
BstMBI GATC 3 cut(s) 521, 741, 943
BstMWI GCNNNNNNNGC 2 cut(s) 275, 609
BstNI CCWGG 3 cut(s) 734, 832, 918
BstNSI RCATGY 1 cut(s) 849
BstPAI GACNNNNGTC 1 cut(s) 257
BstPI GGTNACC 1 cut(s) 920
BstSCI CCNGG 4 cut(s) 691, 732, 830, 916
BstSFI CTRYAG 1 cut(s) 837
BstV1I GCAGC 4 cut(s) 82, 262, 596, 886
BstX2I RGATCY 1 cut(s) 521
BstYI RGATCY 1 cut(s) 521
Bsu15I ATCGAT 1 cut(s) 942
BsuTUI ATCGAT 1 cut(s) 942
BtsCI GGATG 5 cut(s) 246, 417, 573, 883, 886
BtsIMutI CAGTG 2 cut(s) 623, 760
Cac8I GCNNGC 2 cut(s) 165, 582
CfoI GCGC 1 cut(s) 416
ClaI ATCGAT 1 cut(s) 942
CsiI ACCWGGT 1 cut(s) 916
Csp6I GTAC 1 cut(s) 801
CviAII CATG 2 cut(s) 65, 846
CviQI GTAC 1 cut(s) 801
DdeI CTNAG 6 cut(s) 221, 271, 428, 505, 604, 933
DinI GGCGCC 1 cut(s) 415
DpnI GATC 3 cut(s) 523, 743, 945
DpnII GATC 3 cut(s) 521, 741, 943
Eam1104I CTCTTC 1 cut(s) 775
EarI CTCTTC 1 cut(s) 775
Eco32I GATATC 1 cut(s) 718
Eco57I CTGAAG 1 cut(s) 915
Eco91I GGTNACC 1 cut(s) 920
EcoO65I GGTNACC 1 cut(s) 920
EcoRII CCWGG 3 cut(s) 732, 830, 916
EcoRV GATATC 1 cut(s) 718
EgeI GGCGCC 1 cut(s) 415
EheI GGCGCC 1 cut(s) 415
FaeI CATG 2 cut(s) 68, 849
FalI AAGNNNNNCTT 2 cut(s) 769, 801
FatI CATG 2 cut(s) 64, 845
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 1 cut(s) 486
Fnu4HI GCNGC 4 cut(s) 71, 276, 585, 900
FokI GGATG 5 cut(s) 253, 404, 560, 873, 890
Fsp4HI GCNGC 4 cut(s) 71, 276, 585, 900
FspBI CTAG 3 cut(s) 41, 114, 326
GlaI GCGC 1 cut(s) 415
GluI GCNGC 4 cut(s) 71, 276, 585, 900
GsuI CTGGAG 2 cut(s) 276, 814
HaeII RGCGCY 1 cut(s) 417
HapII CCGG 1 cut(s) 693
HhaI GCGC 1 cut(s) 416
Hin1I GRCGYC 1 cut(s) 414
Hin1II CATG 2 cut(s) 68, 849
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HindIII AAGCTT 2 cut(s) 161, 662
HinfI GANTC 8 cut(s) 195, 258, 483, 557, 634, 696, 765, 773
HpaII CCGG 1 cut(s) 693
HphI GGTGA 4 cut(s) 169, 190, 382, 932
Hpy166II GTNNAC 2 cut(s) 17, 487
Hpy188I TCNGA 3 cut(s) 194, 508, 895
Hpy188III TCNNGA 3 cut(s) 606, 638, 777
Hpy8I GTNNAC 2 cut(s) 17, 487
HpyAV CCTTC 3 cut(s) 47, 434, 542
HpyCH4III ACNGT 1 cut(s) 805
HpyCH4V TGCA 5 cut(s) 317, 434, 825, 849, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 609
HpyF3I CTNAG 6 cut(s) 221, 271, 428, 505, 604, 933
Hsp92I GRCGYC 1 cut(s) 414
Hsp92II CATG 2 cut(s) 68, 849
HspAI GCGC 1 cut(s) 414
KasI GGCGCC 1 cut(s) 413
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 3 cut(s) 521, 741, 943
LguI GCTCTTC 1 cut(s) 775
Lsp1109I GCAGC 4 cut(s) 82, 262, 596, 886
LweI GCATC 2 cut(s) 631, 948
MabI ACCWGGT 1 cut(s) 916
MaeI CTAG 3 cut(s) 41, 114, 326
MaeIII GTNAC 3 cut(s) 558, 755, 920
MalI GATC 3 cut(s) 523, 743, 945
MboI GATC 3 cut(s) 521, 741, 943
MboII GAAGA 3 cut(s) 156, 760, 792
MflI RGATCY 1 cut(s) 521
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 8 cut(s) 263, 318, 335, 340, 500, 510, 588, 927
Mly113I GGCGCC 1 cut(s) 414
MlyI GAGTC 5 cut(s) 267, 492, 566, 690, 782
MnlI CCTC 6 cut(s) 211, 280, 541, 740, 901, 901
MseI TTAA 3 cut(s) 126, 338, 666
MslI CAYNNNNRTG 1 cut(s) 239
MspA1I CMGCKG 2 cut(s) 182, 275
MspI CCGG 1 cut(s) 693
MspR9I CCNGG 4 cut(s) 693, 734, 832, 918
MvaI CCWGG 3 cut(s) 734, 832, 918
MwoI GCNNNNNNNGC 2 cut(s) 275, 609
NarI GGCGCC 1 cut(s) 414
NciI CCSGG 1 cut(s) 693
NdeII GATC 3 cut(s) 521, 741, 943
NlaIII CATG 2 cut(s) 68, 849
NlaIV GGNNCC 2 cut(s) 415, 439
NmuCI GTSAC 3 cut(s) 558, 755, 920
NspI RCATGY 1 cut(s) 849
PciSI GCTCTTC 1 cut(s) 775
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PfeI GAWTC 3 cut(s) 195, 634, 765
PflMI CCANNNNNTGG 1 cut(s) 479
PfoI TCCNGGA 2 cut(s) 691, 732
PkrI GCNGC 4 cut(s) 72, 277, 586, 901
PleI GAGTC 5 cut(s) 266, 491, 565, 690, 781
PluTI GGCGCC 1 cut(s) 417
PpsI GAGTC 5 cut(s) 266, 491, 565, 690, 781
PshAI GACNNNNGTC 1 cut(s) 257
PsiI TTATAA 2 cut(s) 291, 456
Psp6I CCWGG 3 cut(s) 732, 830, 916
PspEI GGTNACC 1 cut(s) 920
PspGI CCWGG 3 cut(s) 732, 830, 916
PspN4I GGNNCC 2 cut(s) 415, 439
PsuI RGATCY 1 cut(s) 521
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 802
RsaNI GTAC 1 cut(s) 801
RseI CAYNNNNRTG 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 775
SaqAI TTAA 3 cut(s) 126, 338, 666
SatI GCNGC 4 cut(s) 71, 276, 585, 900
Sau3AI GATC 3 cut(s) 521, 741, 943
SchI GAGTC 5 cut(s) 267, 492, 566, 690, 782
ScrFI CCNGG 4 cut(s) 693, 734, 832, 918
SduI GDGCHC 1 cut(s) 822
SexAI ACCWGGT 1 cut(s) 916
SfaNI GCATC 2 cut(s) 631, 948
SfcI CTRYAG 1 cut(s) 837
SfoI GGCGCC 1 cut(s) 415
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 31, 698
SmoI CTYRAG 2 cut(s) 31, 698
Sse9I AATT 8 cut(s) 263, 318, 335, 340, 500, 510, 588, 927
SsiI CCGC 2 cut(s) 109, 180
SspDI GGCGCC 1 cut(s) 413
SspMI CTAG 3 cut(s) 41, 114, 326
StyD4I CCNGG 4 cut(s) 691, 732, 830, 916
TaaI ACNGT 1 cut(s) 805
TaqI TCGA 3 cut(s) 714, 771, 942
TasI AATT 8 cut(s) 263, 318, 335, 340, 500, 510, 588, 927
TfiI GAWTC 3 cut(s) 195, 634, 765
Tru1I TTAA 3 cut(s) 126, 338, 666
Tru9I TTAA 3 cut(s) 126, 338, 666
TscAI CASTG 2 cut(s) 623, 760
TseFI GTSAC 3 cut(s) 558, 755, 920
TseI GCWGC 4 cut(s) 70, 275, 584, 899
Tsp45I GTSAC 3 cut(s) 558, 755, 920
TspDTI ATGAA 3 cut(s) 439, 526, 875
TspRI CASTG 2 cut(s) 623, 760
Van91I CCANNNNNTGG 1 cut(s) 479
XapI RAATTY 2 cut(s) 263, 510
XceI RCATGY 1 cut(s) 849
XmiI GTMKAC 1 cut(s) 486
XspI CTAG 3 cut(s) 41, 114, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.