Rw3G015870

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0006535
Physical Location & Seq
Forward (+)
16430377 .. 16439012
8636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015870.1

Sequence Viewer

Length: 1053 bp
ATGATAGTTTCCGGTAAACTCTTGGTTTCCTTGAGCCTTCTAGTTCTTGTTTTCAAAGTGTCTCATGGGCAGCCTTTAGTTCCTGCACTCATCATCTTTGGGGACTCTGTTGCTGATGTGGGAAATAATAACAACCGCACTACACTAGTGAAGGCAAACTTCCCTCCTTATGGAAGAGACTTCGTTAATCATCAACCGACTGGAAGATTTTGTAATGGGAAGCTTGCCGCAGACTTCACCGCTGAATATCTCGGATTCACCTCATACCCACCAGCTTTCCTTAGCCAAGAAACCACAGGGATGAGCATTTTGACTGGAGTCAACTTTGCCTCAGCTGCTTCTGGCTATTATAACTGTACAGCCCAGTTATGGGGTGCAATTTCACTAGACCAACAATTAAATTACTACAATGAGTATCAAAGTATAGTGGTGAATATGGTAGGGGAAGCCGAAGCCAATGCCATATTCTCTGGCGCCATCTACCTTCTTAGTGCAGGAACCAGCGATTTCATTCAGAACTACTACATCAATCCATTTCTTAGAGGCTACACACCTGACCAATTCTCAGAAATTCTAATGAGATCCTACTCCTCTTTCATTCAGAAGTTATATGGACTCGGAGCACGAAGGATTGGAGTCACAAACTTACCACCTATAGGATGTTTGCCAGCAGCAATTACCCTATTTGGCTCAGGAAGCAACCAGTGGAACATAAGATTGAATCAAGATGCCATTTTATTCAATAACAAGCTTAACAACACATCTCAAACTTTGCAGAAGAGTCTTCCCGGACTCAAGCTCGTAGTTTTTGATATCTACCAGCCCCTCCTGGATATGATCACAAACCCCAGTGACAATGGGTTCTTCGAGTCAAGAAGAGCTTGTTGTGGAACAGGTACATTTGAAACATCTGTGCTCTGCAACTCTAGGTCTGTAGGAACATGCAACAATGCAACTGGTTATGTGTTCTGGGATGGATTTCATCCCTCTGAAGCTGCCAATGAGGTCTTAGCTGGTGATCTACTTGCACAGGGCATCGATCTCATATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

38.07

Weight (kDa)

5.2

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 338 3.9e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 473
AciI CCGC 3 cut(s) 136, 228, 240
AclWI GGATC 1 cut(s) 576
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 1011
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 2 cut(s) 358, 898
AfiI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
AgsI TTSAA 4 cut(s) 55, 721, 742, 905
AhlI ACTAGT 1 cut(s) 145
AjnI CCWGG 1 cut(s) 828
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AluBI AGCT 8 cut(s) 223, 275, 335, 751, 799, 881, 995, 1013
AluI AGCT 8 cut(s) 223, 275, 335, 751, 799, 881, 995, 1013
Alw21I GWGCWC 2 cut(s) 625, 918
Alw26I GTCTC 2 cut(s) 66, 171
AlwI GGATC 1 cut(s) 576
ApeKI GCWGC 4 cut(s) 70, 335, 671, 995
ApoI RAATTY 1 cut(s) 570
AspLEI GCGC 1 cut(s) 476
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 4 cut(s) 229, 250, 442, 1028
BanI GGYRCC 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 2 cut(s) 625, 918
BbvCI CCTCAGC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 82, 322, 683, 982
BccI CCATC 2 cut(s) 485, 968
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 1 cut(s) 830
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 66, 171
BcuI ACTAGT 1 cut(s) 145
BfaI CTAG 4 cut(s) 41, 146, 386, 927
BfmI CTRYAG 2 cut(s) 654, 933
BfoI RGCGCY 1 cut(s) 477
BisI GCNGC 5 cut(s) 71, 228, 336, 672, 996
BlsI GCNGC 5 cut(s) 72, 229, 337, 673, 997
Bme1390I CCNGG 2 cut(s) 789, 830
BmiI GGNNCC 2 cut(s) 475, 499
BmrFI CCNGG 2 cut(s) 789, 830
BmrI ACTGGG 2 cut(s) 358, 843
BmsI GCATC 2 cut(s) 718, 1044
BmuI ACTGGG 2 cut(s) 358, 843
BoxI GACNNNNGTC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 776
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 3 cut(s) 281, 331, 691
BpuEI CTTGAG 2 cut(s) 52, 779
BpuMI CCSGG 1 cut(s) 789
Bsa29I ATCGAT 1 cut(s) 1038
BsaHI GRCGYC 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
Bse1I ACTGG 6 cut(s) 205, 319, 364, 703, 849, 961
BseBI CCWGG 1 cut(s) 830
BseCI ATCGAT 1 cut(s) 1038
BseGI GGATG 4 cut(s) 306, 665, 979, 982
BseLI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
BseMII CTCAG 3 cut(s) 345, 579, 705
BseNI ACTGG 6 cut(s) 205, 319, 364, 703, 849, 961
BseRI GAGGAG 1 cut(s) 580
BseXI GCAGC 4 cut(s) 82, 322, 683, 982
BsgI GTGCAG 2 cut(s) 69, 513
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 1038
BsiHKAI GWGCWC 2 cut(s) 625, 918
BsiSI CCGG 2 cut(s) 12, 789
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
BsmAI GTCTC 2 cut(s) 66, 171
BsmFI GGGAC 1 cut(s) 116
Bsp1286I GDGCHC 2 cut(s) 625, 918
Bsp1407I TGTACA 1 cut(s) 356
Bsp143I GATC 4 cut(s) 581, 837, 1018, 1039
BspACI CCGC 3 cut(s) 136, 228, 240
BspCNI CTCAG 3 cut(s) 344, 578, 704
BspDI ATCGAT 1 cut(s) 1038
BspLI GGNNCC 2 cut(s) 475, 499
BspPI GGATC 1 cut(s) 576
BspQI GCTCTTC 1 cut(s) 871
BspT107I GGYRCC 1 cut(s) 473
BsrGI TGTACA 1 cut(s) 356
BsrI ACTGG 6 cut(s) 205, 319, 364, 703, 849, 961
BssMI GATC 4 cut(s) 581, 837, 1018, 1039
BssNI GRCGYC 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 830
Bst4CI ACNGT 1 cut(s) 356
Bst6I CTCTTC 3 cut(s) 169, 773, 871
BstACI GRCGYC 1 cut(s) 474
BstAUI TGTACA 1 cut(s) 356
BstC8I GCNNGC 2 cut(s) 225, 669
BstDEI CTNAG 7 cut(s) 281, 331, 488, 539, 565, 691, 1009
BstF5I GGATG 4 cut(s) 306, 665, 979, 982
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 4 cut(s) 584, 840, 1021, 1042
BstMAI GTCTC 2 cut(s) 66, 171
BstMBI GATC 4 cut(s) 581, 837, 1018, 1039
BstMWI GCNNNNNNNGC 2 cut(s) 335, 696
BstNI CCWGG 1 cut(s) 830
BstNSI RCATGY 1 cut(s) 945
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 787, 828
BstSFI CTRYAG 2 cut(s) 654, 933
BstV1I GCAGC 4 cut(s) 82, 322, 683, 982
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
Bsu15I ATCGAT 1 cut(s) 1038
BsuTUI ATCGAT 1 cut(s) 1038
BtsCI GGATG 4 cut(s) 306, 665, 979, 982
BtsIMutI CAGTG 2 cut(s) 710, 856
Cac8I GCNNGC 2 cut(s) 225, 669
CfoI GCGC 1 cut(s) 476
ClaI ATCGAT 1 cut(s) 1038
Csp6I GTAC 2 cut(s) 357, 897
CviAII CATG 2 cut(s) 65, 942
CviQI GTAC 2 cut(s) 357, 897
DdeI CTNAG 7 cut(s) 281, 331, 488, 539, 565, 691, 1009
DinI GGCGCC 1 cut(s) 475
DpnI GATC 4 cut(s) 583, 839, 1020, 1041
DpnII GATC 4 cut(s) 581, 837, 1018, 1039
Eam1104I CTCTTC 3 cut(s) 169, 773, 871
EarI CTCTTC 3 cut(s) 169, 773, 871
Eco32I GATATC 1 cut(s) 814
Eco57I CTGAAG 1 cut(s) 1011
EcoRII CCWGG 1 cut(s) 828
EcoRV GATATC 1 cut(s) 814
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
FaeI CATG 2 cut(s) 68, 945
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 865, 897
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 64, 941
FbaI TGATCA 1 cut(s) 837
Fnu4HI GCNGC 5 cut(s) 71, 228, 336, 672, 996
FokI GGATG 4 cut(s) 313, 672, 969, 986
Fsp4HI GCNGC 5 cut(s) 71, 228, 336, 672, 996
FspBI CTAG 4 cut(s) 41, 146, 386, 927
GlaI GCGC 1 cut(s) 475
GluI GCNGC 5 cut(s) 71, 228, 336, 672, 996
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 477
HapII CCGG 2 cut(s) 12, 789
HhaI GCGC 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 2 cut(s) 68, 945
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 2 cut(s) 221, 749
HinfI GANTC 9 cut(s) 104, 255, 318, 615, 636, 721, 781, 792, 869
HpaII CCGG 2 cut(s) 12, 789
HphI GGTGA 4 cut(s) 229, 250, 442, 1028
Hpy166II GTNNAC 2 cut(s) 17, 322
Hpy188I TCNGA 6 cut(s) 254, 516, 568, 603, 620, 991
Hpy188III TCNNGA 4 cut(s) 693, 725, 873, 1050
Hpy8I GTNNAC 2 cut(s) 17, 322
HpyAV CCTTC 4 cut(s) 47, 145, 494, 621
HpyCH4III ACNGT 1 cut(s) 356
HpyCH4V TGCA 8 cut(s) 86, 377, 494, 775, 921, 945, 953, 1028
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 696
HpyF3I CTNAG 7 cut(s) 281, 331, 488, 539, 565, 691, 1009
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 2 cut(s) 68, 945
HspAI GCGC 1 cut(s) 474
KasI GGCGCC 1 cut(s) 473
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 4 cut(s) 581, 837, 1018, 1039
LguI GCTCTTC 1 cut(s) 871
LmnI GCTCC 1 cut(s) 620
Lsp1109I GCAGC 4 cut(s) 82, 322, 683, 982
LweI GCATC 2 cut(s) 718, 1044
MaeI CTAG 4 cut(s) 41, 146, 386, 927
MaeIII GTNAC 2 cut(s) 637, 851
MalI GATC 4 cut(s) 583, 839, 1020, 1041
MboI GATC 4 cut(s) 581, 837, 1018, 1039
MboII GAAGA 6 cut(s) 186, 216, 776, 790, 856, 888
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 2 cut(s) 625, 918
MluCI AATT 6 cut(s) 378, 395, 400, 560, 570, 675
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 7 cut(s) 98, 327, 609, 645, 786, 790, 878
MnlI CCTC 8 cut(s) 174, 271, 340, 536, 601, 836, 997, 997
MseI TTAA 3 cut(s) 186, 398, 753
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 242, 335
MspI CCGG 2 cut(s) 12, 789
MspR9I CCNGG 2 cut(s) 789, 830
MvaI CCWGG 1 cut(s) 830
MwoI GCNNNNNNNGC 2 cut(s) 335, 696
NarI GGCGCC 1 cut(s) 474
NciI CCSGG 1 cut(s) 789
NdeII GATC 4 cut(s) 581, 837, 1018, 1039
NlaIII CATG 2 cut(s) 68, 945
NlaIV GGNNCC 2 cut(s) 475, 499
NmuCI GTSAC 2 cut(s) 637, 851
NspI RCATGY 1 cut(s) 945
PciSI GCTCTTC 1 cut(s) 871
PfeI GAWTC 2 cut(s) 255, 721
PfoI TCCNGGA 2 cut(s) 787, 828
PkrI GCNGC 5 cut(s) 72, 229, 337, 673, 997
PleI GAGTC 7 cut(s) 98, 326, 609, 644, 786, 789, 877
PluTI GGCGCC 1 cut(s) 477
PpsI GAGTC 7 cut(s) 98, 326, 609, 644, 786, 789, 877
PshAI GACNNNNGTC 1 cut(s) 317
PsiI TTATAA 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 828
PspGI CCWGG 1 cut(s) 828
PspN4I GGNNCC 2 cut(s) 475, 499
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 2 cut(s) 358, 898
RsaNI GTAC 2 cut(s) 357, 897
RseI CAYNNNNRTG 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 871
SaqAI TTAA 3 cut(s) 186, 398, 753
SatI GCNGC 5 cut(s) 71, 228, 336, 672, 996
Sau3AI GATC 4 cut(s) 581, 837, 1018, 1039
SchI GAGTC 7 cut(s) 98, 327, 609, 645, 786, 790, 878
ScrFI CCNGG 2 cut(s) 789, 830
SduI GDGCHC 2 cut(s) 625, 918
SfaNI GCATC 2 cut(s) 718, 1044
SfcI CTRYAG 2 cut(s) 654, 933
SfoI GGCGCC 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 31, 794
SmoI CTYRAG 2 cut(s) 31, 794
SpeI ACTAGT 1 cut(s) 145
Sse9I AATT 6 cut(s) 378, 395, 400, 560, 570, 675
SsiI CCGC 3 cut(s) 136, 228, 240
SspDI GGCGCC 1 cut(s) 473
SspMI CTAG 4 cut(s) 41, 146, 386, 927
StyD4I CCNGG 2 cut(s) 787, 828
TaaI ACNGT 1 cut(s) 356
TaqI TCGA 2 cut(s) 867, 1038
TasI AATT 6 cut(s) 378, 395, 400, 560, 570, 675
TatI WGTACW 1 cut(s) 356
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 2 cut(s) 255, 721
Tru1I TTAA 3 cut(s) 186, 398, 753
Tru9I TTAA 3 cut(s) 186, 398, 753
TscAI CASTG 2 cut(s) 710, 856
TseFI GTSAC 2 cut(s) 637, 851
TseI GCWGC 4 cut(s) 70, 335, 671, 995
Tsp45I GTSAC 2 cut(s) 637, 851
TspDTI ATGAA 3 cut(s) 499, 586, 971
TspRI CASTG 2 cut(s) 710, 856
XapI RAATTY 1 cut(s) 570
XceI RCATGY 1 cut(s) 945
XspI CTAG 4 cut(s) 41, 146, 386, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.