AT5G03810

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006535
Physical Location & Seq
Reverse (-)
1013939 .. 1015345
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03810.1

Sequence Viewer

Length: 1062 bp
ATGAAGATGTTTATTACAATGTCGATGTGCTTATCGGTTATCGCCTGTTTCTACGCCGGCGTTGGGACCGGTGAGACACTTGTACCGGCTCTAATCATAATGGGAGATTCAGTTGTTGATGCCGGTAACAACAACCACCGTATTACCCTCGTCAAAGCCAATTTTCCTCCCTACGGACGTGACTTCGTCGCCCACAGTGCAACTGGCCGTTTCTCTAACGGAAAACTTGCCACAGACTTTACCGCTGAGAATTTAGGGTTTACATCTTACCCGGTGGCTTACCTTAGCCAAGAAGCAAATGAGACAAATTTGTTAACCGGAGCCAATTTCGCATCTGGAGCTTCCGGTTTTGACGACGCAACTGCCATATTTTACAATGCAATAACATTGAGCCAGCAGCTGAAAAACTACAAAGAGTACCAAAACAAGGTGACGAACATTGTGGGAAAAGAAAGAGCAAATGAGATATTCTCAGGTGCAATTCATCTTCTAAGCACAGGATCAAGCGATTTTCTTCAAAGTTATTACATCAATCCGATCCTTAACCGGATTTTCACGCCTGATCAATACTCAGATCATCTCTTGAGATCCTATTCCACCTTTGTCCAAAATTTGTATGGTTTAGGGGCTAGGAGGATTGGAGTGACGACGTTACCGCCATTAGGTTGTTTACCAGCAGCGATAACCCTGTTTGGTGGGGTCGGGAACAATATGTGCGTGGAAAGACTAAACCAGGATGCAGTTTCCTTCAATACGAAACTCAACAACACGTCTATTAACTTAACCAACAATCTTCCCGGTCTGAAGTTGGTCGTCTTTGACATCTATAATCCTCTATTGAACATGGTCATAAACCCTGTCGAGTATGGGTTTTTCGAATCAAGAAGGGCTTGTTGTGGAACGGGTACAATGGAAACTTCGTTCCTATGTAATGCATTGTCGGTGGGTACATGCTCGAATGCTACGAATTACGTGTTTTGGGATGGTTTTCATCCATCCGAGGCAGCCAATCGTGTTATCGCAAATAATCTTCTTGTCCAAGGAATACCCCTCATCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

353

Amino Acids

38.35

Weight (kDa)

5.95

Isoelectric Point (pI)

28.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 343 1.1e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 243, 656
AclWI GGATC 3 cut(s) 508, 532, 582
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 3 cut(s) 250, 307, 610
AcuI CTGAAG 1 cut(s) 824
AfaI GTAC 4 cut(s) 84, 419, 907, 949
AfiI CCNNNNNNNGG 4 cut(s) 63, 173, 427, 662
AflIII ACRYGT 2 cut(s) 768, 972
AgeI ACCGGT 1 cut(s) 68
AgsI TTSAA 3 cut(s) 518, 751, 841
AjiI CACGTC 2 cut(s) 179, 771
AjnI CCWGG 1 cut(s) 732
AloI GAACNNNNNNTCC 2 cut(s) 905, 937
AluBI AGCT 2 cut(s) 341, 400
AluI AGCT 2 cut(s) 341, 400
Alw26I GTCTC 2 cut(s) 68, 296
AlwI GGATC 3 cut(s) 508, 532, 582
AlwNI CAGNNNCTG 1 cut(s) 400
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 397, 677, 1004
ApoI RAATTY 3 cut(s) 250, 307, 610
AsiGI ACCGGT 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 2 cut(s) 272, 798
AsuHPI GGTGA 2 cut(s) 83, 442
AsuII TTCGAA 1 cut(s) 876
AvaII GGWCC 1 cut(s) 66
BaeI ACNNNNGTAYC 2 cut(s) 66, 99
BbvI GCAGC 3 cut(s) 409, 689, 1016
BccI CCATC 2 cut(s) 977, 1003
BceAI ACGGC 1 cut(s) 192
BcgI CGANNNNNNTGC 2 cut(s) 344, 378
BciT130I CCWGG 1 cut(s) 734
BclI TGATCA 1 cut(s) 562
BcnI CCSGG 2 cut(s) 272, 798
BcoDI GTCTC 2 cut(s) 68, 296
BfaI CTAG 1 cut(s) 630
BisI GCNGC 3 cut(s) 398, 678, 1005
BlsI GCNGC 3 cut(s) 399, 679, 1006
Bme1390I CCNGG 3 cut(s) 272, 734, 798
Bme18I GGWCC 1 cut(s) 66
BmgBI CACGTC 2 cut(s) 179, 771
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 2 cut(s) 67, 322
BmrFI CCNGG 3 cut(s) 272, 734, 798
BmsI GCATC 3 cut(s) 109, 341, 727
BplI GAGNNNNNCTC 2 cut(s) 455, 487
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 284
Bpu14I TTCGAA 1 cut(s) 876
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 2 cut(s) 272, 798
BsaAI YACGTR 1 cut(s) 973
BsaJI CCNNGG 2 cut(s) 999, 1039
BsaWI WCCGGW 4 cut(s) 68, 317, 344, 546
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 173, 427, 662
Bse118I RCCGGY 4 cut(s) 56, 68, 85, 122
Bse1I ACTGG 1 cut(s) 208
BseBI CCWGG 1 cut(s) 734
BseDI CCNNGG 2 cut(s) 999, 1039
BseGI GGATG 4 cut(s) 742, 988, 991, 995
BseLI CCNNNNNNNGG 4 cut(s) 63, 173, 427, 662
BseMII CTCAG 3 cut(s) 237, 486, 585
BseNI ACTGG 1 cut(s) 208
BseXI GCAGC 3 cut(s) 409, 689, 1016
BshFI GGCC 1 cut(s) 207
BshTI ACCGGT 1 cut(s) 68
BsiSI CCGG 9 cut(s) 57, 69, 86, 123, 272, 318, 345, 547, 798
BslFI GGGAC 1 cut(s) 79
BslI CCNNNNNNNGG 4 cut(s) 63, 173, 427, 662
BsmAI GTCTC 2 cut(s) 68, 296
BsmFI GGGAC 1 cut(s) 79
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 876
Bsp143I GATC 5 cut(s) 500, 537, 562, 574, 587
BspACI CCGC 2 cut(s) 243, 656
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 3 cut(s) 238, 485, 584
BspLI GGNNCC 2 cut(s) 67, 322
BspPI GGATC 3 cut(s) 508, 532, 582
BspT104I TTCGAA 1 cut(s) 876
BsrFI RCCGGY 4 cut(s) 56, 68, 85, 122
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 4 cut(s) 56, 68, 85, 122
BssECI CCNNGG 2 cut(s) 999, 1039
BssMI GATC 5 cut(s) 500, 537, 562, 574, 587
BssT1I CCWWGG 1 cut(s) 1039
Bst2UI CCWGG 1 cut(s) 734
Bst4CI ACNGT 2 cut(s) 140, 197
BstBAI YACGTR 1 cut(s) 973
BstBI TTCGAA 1 cut(s) 876
BstC8I GCNNGC 2 cut(s) 58, 395
BstDEI CTNAG 5 cut(s) 246, 284, 472, 491, 571
BstF5I GGATG 4 cut(s) 742, 988, 991, 995
BstKTI GATC 5 cut(s) 503, 540, 565, 577, 590
BstMAI GTCTC 2 cut(s) 68, 296
BstMBI GATC 5 cut(s) 500, 537, 562, 574, 587
BstMWI GCNNNNNNNGC 3 cut(s) 197, 329, 338
BstNI CCWGG 1 cut(s) 734
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 3 cut(s) 270, 732, 796
BstV1I GCAGC 3 cut(s) 409, 689, 1016
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
BsuRI GGCC 1 cut(s) 207
BtrI CACGTC 2 cut(s) 179, 771
BtsCI GGATG 4 cut(s) 742, 988, 991, 995
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 2 cut(s) 58, 395
CaiI CAGNNNCTG 1 cut(s) 400
Cfr10I RCCGGY 4 cut(s) 56, 68, 85, 122
Cfr13I GGNCC 1 cut(s) 66
CseI GACGC 1 cut(s) 365
Csp6I GTAC 4 cut(s) 83, 418, 906, 948
CspAI ACCGGT 1 cut(s) 68
CviAII CATG 2 cut(s) 844, 951
CviQI GTAC 4 cut(s) 83, 418, 906, 948
DdeI CTNAG 5 cut(s) 246, 284, 472, 491, 571
DpnI GATC 5 cut(s) 502, 539, 564, 576, 589
DpnII GATC 5 cut(s) 500, 537, 562, 574, 587
EaeI YGGCCR 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 1039
Eco47I GGWCC 1 cut(s) 66
Eco57I CTGAAG 1 cut(s) 824
EcoRII CCWGG 1 cut(s) 732
EcoT14I CCWWGG 1 cut(s) 1039
EcoT22I ATGCAT 1 cut(s) 937
ErhI CCWWGG 1 cut(s) 1039
FaeI CATG 2 cut(s) 847, 954
FalI AAGNNNNNCTT 2 cut(s) 874, 906
FaqI GGGAC 1 cut(s) 79
FatI CATG 2 cut(s) 843, 950
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 3 cut(s) 398, 678, 1005
FokI GGATG 4 cut(s) 749, 978, 982, 995
Fsp4HI GCNGC 3 cut(s) 398, 678, 1005
FspBI CTAG 1 cut(s) 630
GluI GCNGC 3 cut(s) 398, 678, 1005
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 1 cut(s) 207
HapII CCGG 9 cut(s) 57, 69, 86, 123, 272, 318, 345, 547, 798
HgaI GACGC 1 cut(s) 365
Hin1II CATG 2 cut(s) 847, 954
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 2 cut(s) 107, 878
HpaI GTTAAC 1 cut(s) 315
HpaII CCGG 9 cut(s) 57, 69, 86, 123, 272, 318, 345, 547, 798
HphI GGTGA 2 cut(s) 83, 442
Hpy166II GTNNAC 3 cut(s) 261, 315, 671
Hpy188I TCNGA 4 cut(s) 537, 574, 804, 1000
Hpy188III TCNNGA 4 cut(s) 336, 583, 703, 882
Hpy8I GTNNAC 3 cut(s) 261, 315, 671
Hpy99I CGWCG 3 cut(s) 191, 359, 652
HpyAV CCTTC 2 cut(s) 757, 879
HpyCH4III ACNGT 2 cut(s) 140, 197
HpyCH4IV ACGT 4 cut(s) 178, 650, 770, 972
HpyCH4V TGCA 5 cut(s) 200, 380, 479, 740, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 329, 338
HpyF3I CTNAG 5 cut(s) 246, 284, 472, 491, 571
HpySE526I ACGT 4 cut(s) 178, 650, 770, 972
Hsp92II CATG 2 cut(s) 847, 954
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
Ksp22I TGATCA 1 cut(s) 562
KspAI GTTAAC 1 cut(s) 315
Kzo9I GATC 5 cut(s) 500, 537, 562, 574, 587
LmnI GCTCC 2 cut(s) 320, 338
Lsp1109I GCAGC 3 cut(s) 409, 689, 1016
LweI GCATC 3 cut(s) 109, 341, 727
MaeI CTAG 1 cut(s) 630
MaeII ACGT 4 cut(s) 178, 650, 770, 972
MaeIII GTNAC 5 cut(s) 125, 179, 430, 643, 651
MalI GATC 5 cut(s) 502, 539, 564, 576, 589
MboI GATC 5 cut(s) 500, 537, 562, 574, 587
MboII GAAGA 5 cut(s) 16, 479, 506, 785, 1022
MflI RGATCY 1 cut(s) 587
MluCI AATT 7 cut(s) 160, 250, 307, 325, 480, 610, 967
MnlI CCTC 6 cut(s) 158, 177, 627, 843, 994, 1061
Mph1103I ATGCAT 1 cut(s) 937
MreI CGCCGGCG 1 cut(s) 56
MroNI GCCGGC 1 cut(s) 56
MseI TTAA 5 cut(s) 314, 543, 777, 782, 1060
MspA1I CMGCKG 2 cut(s) 245, 400
MspI CCGG 9 cut(s) 57, 69, 86, 123, 272, 318, 345, 547, 798
MspR9I CCNGG 3 cut(s) 272, 734, 798
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 1 cut(s) 734
MwoI GCNNNNNNNGC 3 cut(s) 197, 329, 338
NaeI GCCGGC 1 cut(s) 58
NciI CCSGG 2 cut(s) 272, 798
NdeII GATC 5 cut(s) 500, 537, 562, 574, 587
NgoMIV GCCGGC 1 cut(s) 56
NlaIII CATG 2 cut(s) 847, 954
NlaIV GGNNCC 2 cut(s) 67, 322
NmuCI GTSAC 3 cut(s) 179, 430, 643
NsiI ATGCAT 1 cut(s) 937
NspI RCATGY 1 cut(s) 954
NspV TTCGAA 1 cut(s) 876
PcsI WCGNNNNNNNCGW 1 cut(s) 962
PctI GAATGC 1 cut(s) 964
PdiI GCCGGC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 107, 878
PflFI GACNNNGTC 1 cut(s) 185
PinAI ACCGGT 1 cut(s) 68
PkrI GCNGC 3 cut(s) 399, 679, 1006
Ppu21I YACGTR 1 cut(s) 973
Psp6I CCWGG 1 cut(s) 732
PspGI CCWGG 1 cut(s) 732
PspN4I GGNNCC 2 cut(s) 67, 322
PspPI GGNCC 1 cut(s) 66
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 1 cut(s) 587
PsyI GACNNNGTC 1 cut(s) 185
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 4 cut(s) 84, 419, 907, 949
RsaNI GTAC 4 cut(s) 83, 418, 906, 948
SaqAI TTAA 5 cut(s) 314, 543, 777, 782, 1060
SatI GCNGC 3 cut(s) 398, 678, 1005
Sau3AI GATC 5 cut(s) 500, 537, 562, 574, 587
Sau96I GGNCC 1 cut(s) 66
ScrFI CCNGG 3 cut(s) 272, 734, 798
SfaNI GCATC 3 cut(s) 109, 341, 727
SfuI TTCGAA 1 cut(s) 876
SgrAI CRCCGGYG 1 cut(s) 56
SinI GGWCC 1 cut(s) 66
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 7 cut(s) 160, 250, 307, 325, 480, 610, 967
SsiI CCGC 2 cut(s) 243, 656
SspMI CTAG 1 cut(s) 630
StyD4I CCNGG 3 cut(s) 270, 732, 796
StyI CCWWGG 1 cut(s) 1039
TaaI ACNGT 2 cut(s) 140, 197
TaiI ACGT 4 cut(s) 181, 653, 773, 975
TaqI TCGA 4 cut(s) 23, 861, 876, 956
TasI AATT 7 cut(s) 160, 250, 307, 325, 480, 610, 967
TfiI GAWTC 2 cut(s) 107, 878
Tru1I TTAA 5 cut(s) 314, 543, 777, 782, 1060
Tru9I TTAA 5 cut(s) 314, 543, 777, 782, 1060
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 3 cut(s) 179, 430, 643
TseI GCWGC 3 cut(s) 397, 677, 1004
Tsp45I GTSAC 3 cut(s) 179, 430, 643
TspDTI ATGAA 3 cut(s) 17, 473, 980
TspGWI ACGGA 2 cut(s) 189, 234
TspRI CASTG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 66
XapI RAATTY 3 cut(s) 250, 307, 610
XceI RCATGY 1 cut(s) 954
XcmI CCANNNNNNNNNTGG 2 cut(s) 200, 614
XspI CTAG 1 cut(s) 630
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.