AT5G03820

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006535
Physical Location & Seq
Reverse (-)
1015586 .. 1017514
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G03820.2

Sequence Viewer

Length: 1074 bp
ATGAATAAAATGAAGATGTTTATTATAATGTTGATGACCTTTTCGGTTATCGCCTGTTTCTACGCCGGCGTTGGGACCGGTGAGCCACTTGTACCGGCTCTAATCATAATGGGAGATTCAGTTGTCGATGCCGGCAACAACAACCGCCTCAATACCCTCATCAAAGCCAATTTTCCTCCCTATGGACGTGACTTCTTAGCCCACAATGCCACTGGCCGTTTCTCTAACGGAAAACTTGCCACAGACTTTACCGCTGAGAGTTTAGGGTTCACTTCTTACCCGGTGCCGTATCTTAGCCAAGAAGCTAATGGGACAAATCTGTTAACCGGAGCCAATTTCGCCTCTGGTGCTTCCGGTTATGACGACGGAACTGCCATATTTTACAATGCAATAACATTGAACCAACAGCTGAAAAACTACAAAGAGTACCAAAACAAGGTGACAAACATTGTTGGAAGCGAAAGAGCAAATAAAATATTCTCAGGCGCGATTCATCTTCTAAGCACGGGATCAAGCGATTTTCTTCAAAGCTATTACATCAACCCGATCCTTAACCGGATATTCACGCCTGATCAATACTCAGATCGTCTCATGAAACCCTATTCCACCTTTGTCCAAAATTTGTATGATTTAGGAGCTAGAAAGATTGGAGTGACGACGTTACCGCCATTGGGTTGTTTACCGGCGGCGATAACCTTGTTTGGTGAAACCGGGAACAATAATACCTGCGTGGAAAGACTAAACCAGGATGCGGTTTCCTTCAATACCAAACTCAACAACACGTCTATGAACTTAACCAACAATCTTCCCGGTCTGAAATTGGTCGTCTTTGACATCTATAATCCTCTCTTAAACATGGCCATGAACCCTGTCGAGAATGGGTTTTTTGAATCAAGAAGAGCATGTTGTGGAACCGGAACAGTGGAAACGTCGTTTCTATGTAATGCAAGATCGGTGGGTACATGTTCGAATGCTACGAATTACGTGTTTTGGGATGGTTTTCATCCATCCGAAGCAGCCAATCGTGTTATCGCAAATAATCTTCTTGTCCAAGGAATACCCCTCATCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0048046
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

38.92

Weight (kDa)

6.81

Isoelectric Point (pI)

26.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 63 - 375 3.4e-38 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 734
AccB1I GGYRCC 1 cut(s) 283
AccII CGCG 1 cut(s) 488
AciI CCGC 5 cut(s) 145, 252, 665, 686, 752
AclWI GGATC 2 cut(s) 517, 541
AcoI YGGCCR 2 cut(s) 214, 858
AcsI RAATTY 1 cut(s) 619
AfaI GTAC 3 cut(s) 93, 428, 961
AfiI CCNNNNNNNGG 5 cut(s) 72, 182, 436, 671, 751
AflIII ACRYGT 3 cut(s) 780, 962, 984
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 4 cut(s) 400, 527, 763, 890
AjiI CACGTC 2 cut(s) 188, 783
AjnI CCWGG 1 cut(s) 744
AluBI AGCT 4 cut(s) 305, 409, 531, 638
AluI AGCT 4 cut(s) 305, 409, 531, 638
Alw26I GTCTC 1 cut(s) 593
AlwI GGATC 2 cut(s) 517, 541
AoxI GGCC 2 cut(s) 214, 858
ApeKI GCWGC 1 cut(s) 1016
ApoI RAATTY 1 cut(s) 619
AsiGI ACCGGT 1 cut(s) 77
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 1 cut(s) 75
AsuC2I CCSGG 3 cut(s) 281, 712, 810
AsuHPI GGTGA 3 cut(s) 92, 451, 716
AsuII TTCGAA 1 cut(s) 968
AvaII GGWCC 1 cut(s) 75
BalI TGGCCA 1 cut(s) 860
BanI GGYRCC 1 cut(s) 283
BbvI GCAGC 1 cut(s) 1028
BccI CCATC 2 cut(s) 989, 1015
BceAI ACGGC 2 cut(s) 201, 271
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 1 cut(s) 746
BclI TGATCA 1 cut(s) 571
BcnI CCSGG 3 cut(s) 281, 712, 810
BcoDI GTCTC 1 cut(s) 593
BfaI CTAG 1 cut(s) 639
BfuAI ACCTGC 1 cut(s) 734
BisI GCNGC 2 cut(s) 687, 1017
BlsI GCNGC 2 cut(s) 688, 1018
Bme1390I CCNGG 4 cut(s) 281, 712, 746, 810
Bme18I GGWCC 1 cut(s) 75
BmgBI CACGTC 2 cut(s) 188, 783
BmgT120I GGNCC 1 cut(s) 75
BmiI GGNNCC 4 cut(s) 76, 285, 331, 913
BmrFI CCNGG 4 cut(s) 281, 712, 746, 810
BmsI GCATC 2 cut(s) 118, 739
Bpu14I TTCGAA 1 cut(s) 968
BpuMI CCSGG 3 cut(s) 281, 712, 810
BsaAI YACGTR 1 cut(s) 985
BsaJI CCNNGG 1 cut(s) 1051
BsaWI WCCGGW 5 cut(s) 77, 326, 353, 555, 914
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 5 cut(s) 72, 182, 436, 671, 751
Bse118I RCCGGY 5 cut(s) 65, 77, 94, 131, 682
Bse1I ACTGG 1 cut(s) 217
BseBI CCWGG 1 cut(s) 746
BseDI CCNNGG 1 cut(s) 1051
BseGI GGATG 4 cut(s) 754, 1000, 1003, 1007
BseLI CCNNNNNNNGG 5 cut(s) 72, 182, 436, 671, 751
BseMII CTCAG 3 cut(s) 246, 495, 594
BseNI ACTGG 1 cut(s) 217
BseXI GCAGC 1 cut(s) 1028
Bsh1236I CGCG 1 cut(s) 488
BshFI GGCC 2 cut(s) 216, 860
BshNI GGYRCC 1 cut(s) 283
BshTI ACCGGT 1 cut(s) 77
BslFI GGGAC 2 cut(s) 88, 325
BslI CCNNNNNNNGG 5 cut(s) 72, 182, 436, 671, 751
BsmAI GTCTC 1 cut(s) 593
BsmBI CGTCTC 1 cut(s) 593
BsmFI GGGAC 2 cut(s) 88, 325
BsmI GAATGC 1 cut(s) 976
BsnI GGCC 2 cut(s) 216, 860
Bsp119I TTCGAA 1 cut(s) 968
Bsp143I GATC 5 cut(s) 509, 546, 571, 583, 950
BspACI CCGC 5 cut(s) 145, 252, 665, 686, 752
BspANI GGCC 2 cut(s) 216, 860
BspCNI CTCAG 3 cut(s) 247, 494, 593
BspFNI CGCG 1 cut(s) 488
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 4 cut(s) 76, 285, 331, 913
BspMI ACCTGC 1 cut(s) 734
BspPI GGATC 2 cut(s) 517, 541
BspQI GCTCTTC 1 cut(s) 892
BspT104I TTCGAA 1 cut(s) 968
BspT107I GGYRCC 1 cut(s) 283
BsrFI RCCGGY 5 cut(s) 65, 77, 94, 131, 682
BsrI ACTGG 1 cut(s) 217
BssAI RCCGGY 5 cut(s) 65, 77, 94, 131, 682
BssECI CCNNGG 1 cut(s) 1051
BssMI GATC 5 cut(s) 509, 546, 571, 583, 950
BssT1I CCWWGG 1 cut(s) 1051
Bst2UI CCWGG 1 cut(s) 746
Bst4CI ACNGT 1 cut(s) 922
Bst6I CTCTTC 1 cut(s) 892
BstBAI YACGTR 1 cut(s) 985
BstBI TTCGAA 1 cut(s) 968
BstC8I GCNNGC 2 cut(s) 67, 133
BstDEI CTNAG 6 cut(s) 196, 255, 293, 481, 500, 580
BstF5I GGATG 4 cut(s) 754, 1000, 1003, 1007
BstFNI CGCG 1 cut(s) 488
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 5 cut(s) 512, 549, 574, 586, 953
BstMAI GTCTC 1 cut(s) 593
BstMBI GATC 5 cut(s) 509, 546, 571, 583, 950
BstMWI GCNNNNNNNGC 3 cut(s) 206, 338, 347
BstNI CCWGG 1 cut(s) 746
BstNSI RCATGY 2 cut(s) 906, 966
BstSCI CCNGG 4 cut(s) 279, 710, 744, 808
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 1 cut(s) 1028
BsuRI GGCC 2 cut(s) 216, 860
BtrI CACGTC 2 cut(s) 188, 783
BtsCI GGATG 4 cut(s) 754, 1000, 1003, 1007
BtsIMutI CAGTG 2 cut(s) 210, 927
BveI ACCTGC 1 cut(s) 734
Cac8I GCNNGC 2 cut(s) 67, 133
CciI TCATGA 1 cut(s) 591
CfoI GCGC 1 cut(s) 488
Cfr10I RCCGGY 5 cut(s) 65, 77, 94, 131, 682
Cfr13I GGNCC 1 cut(s) 75
Csp6I GTAC 3 cut(s) 92, 427, 960
CspAI ACCGGT 1 cut(s) 77
CspCI CAANNNNNGTGG 2 cut(s) 936, 971
CviAII CATG 5 cut(s) 592, 856, 862, 903, 963
CviQI GTAC 3 cut(s) 92, 427, 960
DdeI CTNAG 6 cut(s) 196, 255, 293, 481, 500, 580
DpnI GATC 5 cut(s) 511, 548, 573, 585, 952
DpnII GATC 5 cut(s) 509, 546, 571, 583, 950
EaeI YGGCCR 2 cut(s) 214, 858
Eam1104I CTCTTC 1 cut(s) 892
EarI CTCTTC 1 cut(s) 892
Eco130I CCWWGG 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 744
EcoT14I CCWWGG 1 cut(s) 1051
ErhI CCWWGG 1 cut(s) 1051
Esp3I CGTCTC 1 cut(s) 593
FaeI CATG 5 cut(s) 595, 859, 865, 906, 966
FaqI GGGAC 2 cut(s) 88, 325
FatI CATG 5 cut(s) 591, 855, 861, 902, 962
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 2 cut(s) 687, 1017
FokI GGATG 4 cut(s) 761, 990, 994, 1007
Fsp4HI GCNGC 2 cut(s) 687, 1017
FspBI CTAG 1 cut(s) 639
GlaI GCGC 1 cut(s) 487
GluI GCNGC 2 cut(s) 687, 1017
HaeIII GGCC 2 cut(s) 216, 860
HhaI GCGC 1 cut(s) 488
Hin1II CATG 5 cut(s) 595, 859, 865, 906, 966
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HincII GTYRAC 1 cut(s) 324
HindII GTYRAC 1 cut(s) 324
HinfI GANTC 3 cut(s) 116, 490, 890
HpaI GTTAAC 1 cut(s) 324
HphI GGTGA 3 cut(s) 92, 451, 716
Hpy166II GTNNAC 3 cut(s) 270, 324, 680
Hpy188I TCNGA 3 cut(s) 583, 816, 1012
Hpy188III TCNNGA 3 cut(s) 592, 874, 894
Hpy8I GTNNAC 3 cut(s) 270, 324, 680
Hpy99I CGWCG 3 cut(s) 368, 661, 934
HpyAV CCTTC 1 cut(s) 769
HpyCH4III ACNGT 1 cut(s) 922
HpyCH4IV ACGT 5 cut(s) 187, 659, 782, 929, 984
HpyCH4V TGCA 2 cut(s) 389, 947
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 338, 347
HpyF3I CTNAG 6 cut(s) 196, 255, 293, 481, 500, 580
HpySE526I ACGT 5 cut(s) 187, 659, 782, 929, 984
Hsp92II CATG 5 cut(s) 595, 859, 865, 906, 966
HspAI GCGC 1 cut(s) 486
KroI GCCGGC 2 cut(s) 65, 131
KroNI GCCGGC 2 cut(s) 67, 133
Ksp22I TGATCA 1 cut(s) 571
KspAI GTTAAC 1 cut(s) 324
Kzo9I GATC 5 cut(s) 509, 546, 571, 583, 950
LguI GCTCTTC 1 cut(s) 892
LmnI GCTCC 2 cut(s) 329, 635
Lsp1109I GCAGC 1 cut(s) 1028
LweI GCATC 2 cut(s) 118, 739
MaeI CTAG 1 cut(s) 639
MaeII ACGT 5 cut(s) 187, 659, 782, 929, 984
MaeIII GTNAC 4 cut(s) 188, 439, 652, 660
MalI GATC 5 cut(s) 511, 548, 573, 585, 952
MboI GATC 5 cut(s) 509, 546, 571, 583, 950
MboII GAAGA 6 cut(s) 25, 488, 515, 797, 909, 1034
MlsI TGGCCA 1 cut(s) 860
MluCI AATT 5 cut(s) 169, 334, 619, 818, 979
MluNI TGGCCA 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 433
MnlI CCTC 6 cut(s) 158, 167, 186, 352, 855, 1073
Mox20I TGGCCA 1 cut(s) 860
MreI CGCCGGCG 1 cut(s) 65
MroNI GCCGGC 2 cut(s) 65, 131
MscI TGGCCA 1 cut(s) 860
MseI TTAA 5 cut(s) 323, 552, 794, 851, 1072
MslI CAYNNNNRTG 2 cut(s) 785, 860
Msp20I TGGCCA 1 cut(s) 860
MspA1I CMGCKG 2 cut(s) 254, 409
MspR9I CCNGG 4 cut(s) 281, 712, 746, 810
Mva1269I GAATGC 1 cut(s) 976
MvaI CCWGG 1 cut(s) 746
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 3 cut(s) 206, 338, 347
NaeI GCCGGC 2 cut(s) 67, 133
NciI CCSGG 3 cut(s) 281, 712, 810
NdeII GATC 5 cut(s) 509, 546, 571, 583, 950
NgoMIV GCCGGC 2 cut(s) 65, 131
NlaIII CATG 5 cut(s) 595, 859, 865, 906, 966
NlaIV GGNNCC 4 cut(s) 76, 285, 331, 913
NmuCI GTSAC 3 cut(s) 188, 439, 652
NspI RCATGY 2 cut(s) 906, 966
NspV TTCGAA 1 cut(s) 968
PagI TCATGA 1 cut(s) 591
PciI ACATGT 1 cut(s) 962
PciSI GCTCTTC 1 cut(s) 892
PcsI WCGNNNNNNNCGW 1 cut(s) 974
PctI GAATGC 1 cut(s) 976
PdiI GCCGGC 2 cut(s) 67, 133
PfeI GAWTC 3 cut(s) 116, 490, 890
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 2 cut(s) 688, 1018
Ppu21I YACGTR 1 cut(s) 985
PscI ACATGT 1 cut(s) 962
PsiI TTATAA 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 744
PspGI CCWGG 1 cut(s) 744
PspN4I GGNNCC 4 cut(s) 76, 285, 331, 913
PspPI GGNCC 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 409
RsaI GTAC 3 cut(s) 93, 428, 961
RsaNI GTAC 3 cut(s) 92, 427, 960
RseI CAYNNNNRTG 2 cut(s) 785, 860
SapI GCTCTTC 1 cut(s) 892
SaqAI TTAA 5 cut(s) 323, 552, 794, 851, 1072
SatI GCNGC 2 cut(s) 687, 1017
Sau3AI GATC 5 cut(s) 509, 546, 571, 583, 950
Sau96I GGNCC 1 cut(s) 75
ScrFI CCNGG 4 cut(s) 281, 712, 746, 810
SfaNI GCATC 2 cut(s) 118, 739
SfuI TTCGAA 1 cut(s) 968
SgrAI CRCCGGYG 1 cut(s) 65
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 2 cut(s) 785, 860
Sse9I AATT 5 cut(s) 169, 334, 619, 818, 979
SsiI CCGC 5 cut(s) 145, 252, 665, 686, 752
SspI AATATT 1 cut(s) 477
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 4 cut(s) 279, 710, 744, 808
StyI CCWWGG 1 cut(s) 1051
TaaI ACNGT 1 cut(s) 922
TaiI ACGT 5 cut(s) 190, 662, 785, 932, 987
TaqI TCGA 3 cut(s) 126, 873, 968
TasI AATT 5 cut(s) 169, 334, 619, 818, 979
TauI GCSGC 1 cut(s) 689
TfiI GAWTC 3 cut(s) 116, 490, 890
Tru1I TTAA 5 cut(s) 323, 552, 794, 851, 1072
Tru9I TTAA 5 cut(s) 323, 552, 794, 851, 1072
TscAI CASTG 2 cut(s) 217, 927
TseFI GTSAC 3 cut(s) 188, 439, 652
TseI GCWGC 1 cut(s) 1016
Tsp45I GTSAC 3 cut(s) 188, 439, 652
TspDTI ATGAA 7 cut(s) 17, 26, 482, 608, 803, 878, 992
TspGWI ACGGA 2 cut(s) 243, 381
TspRI CASTG 2 cut(s) 217, 927
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 1 cut(s) 619
XceI RCATGY 2 cut(s) 906, 966
XcmI CCANNNNNNNNNTGG 2 cut(s) 209, 305
XspI CTAG 1 cut(s) 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.