RLG00000024172

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006535
Physical Location & Seq
Reverse (-)
32754609 .. 32756961
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024172

Sequence Viewer

Length: 1134 bp
ATGGAGTTTTCAAGTAGTAAACTCTTGGGTTCCTTGAGCTTTCTTGCTCTAGTTTTCACAGTTGTTCTTGGAGACCCTTTAGTTCCAGCTCTTATCATCTTTGGGGACTCTGTAGTCGATGTGGGAAACAATAACAATCTCAACACAGTCATCAAGGCCAACTTCCCTCCTTACGGAAGAGACTTTGTGAATCACCAACCAACTGGAAGATTCTGTAATGGAAAGCTTGCCACCGATTTCACTGCTGAATATCTGGGATTCACTTCATATCCACCAGCTTACCTAAGCCAAGAAGCCAGCGGGAAGAACCTCTTGACTGGAGTCAACTTTGCCTCAGCTGCTTCTGGATTTTATGACCCAACAGCCCAGTTATATGTATGTAAACACCGACATTTAAGTAATTTGTATCTCTTCTTCATCACTATATATCGATATATCACTCTTACAGTTTCAAATTCGATTTCACTGACCCAGCAACTGAATAACTACAAGGAGTATCAAGGTAAAGTGGTGAATCTGGTAGGACAAACTAAAGCCAATGCCATATTCTCTGGAGGCATCCACCTTCTAAGTGCAGGAAGCAGTGATTTCATTCAGAACTACTACATCACTCCCCTCATTACGGTCTATACGCCCGATCAATTCTCGGACATTCTCATTAGATCCTACTCTACATTCATTCAGAATTTGTATGGGTTGGGAGCAAGAAGGATTGGAGTCACAGGCTTACCGCCAACAGGGTGTTTGCCAGCAGCAATCACCCTATTTGGCAATGGAAACAACCAATGCGTCACAAGACTAAACGGAGACGCCATTTCATTCAACAAGAAGCTAAAGAGCACATCTCAAAGTTTGCAGACCAAACTTCCGGGCCTCAAGCTTGTGGTCTTCGATATCTTCCAGCCTCTGCTGGATATGGTCACAAAGCCTAGTGATAATGGGTTCATGGAGTCGAGACGAGCTTGCTGTGGAACAGGAACATTAGAAACCTCTGTGCTTTGCAACTCGAGGTCTATAGGAACATGTAGCAATGTGACTGGTTATGTGTTTTGGGATGGATTCCATCCCTCTCAAGCTGCCAACGAGGTCTTGGCCGGTGATCTACTTCAGCAGGGTTTCGCTCTTATATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

40.97

Weight (kDa)

8.33

Isoelectric Point (pI)

25.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 365 3.3e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AciI CCGC 2 cut(s) 300, 731
AclWI GGATC 1 cut(s) 657
AcoI YGGCCR 1 cut(s) 1092
AcsI RAATTY 2 cut(s) 454, 685
AcuI CTGAAG 1 cut(s) 1091
AcyI GRCGYC 1 cut(s) 810
AfiI CCNNNNNNNGG 3 cut(s) 173, 622, 737
AflIII ACRYGT 1 cut(s) 1022
AgsI TTSAA 3 cut(s) 12, 453, 823
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 9 cut(s) 39, 89, 226, 278, 338, 832, 880, 962, 1076
AluI AGCT 9 cut(s) 39, 89, 226, 278, 338, 832, 880, 962, 1076
Alw21I GWGCWC 1 cut(s) 842
Alw26I GTCTC 4 cut(s) 66, 174, 801, 949
AlwI GGATC 1 cut(s) 657
AlwNI CAGNNNCTG 2 cut(s) 478, 907
Ama87I CYCGRG 1 cut(s) 1006
AoxI GGCC 3 cut(s) 156, 871, 1092
ApeKI GCWGC 3 cut(s) 338, 752, 1076
ApoI RAATTY 2 cut(s) 454, 685
AspS9I GGNCC 1 cut(s) 871
AsuC2I CCSGG 1 cut(s) 870
AsuHPI GGTGA 4 cut(s) 185, 523, 751, 1109
AvaI CYCGRG 1 cut(s) 1006
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbsI GAAGAC 1 cut(s) 880
Bbv12I GWGCWC 1 cut(s) 842
BbvCI CCTCAGC 1 cut(s) 334
BbvI GCAGC 3 cut(s) 325, 764, 1063
BccI CCATC 2 cut(s) 1049, 1071
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BcnI CCSGG 1 cut(s) 870
BcoDI GTCTC 4 cut(s) 66, 174, 801, 949
BfaI CTAG 2 cut(s) 50, 930
BfmI CTRYAG 2 cut(s) 111, 1014
BisI GCNGC 3 cut(s) 339, 753, 1077
BlsI GCNGC 3 cut(s) 340, 754, 1078
Bme1390I CCNGG 1 cut(s) 870
BmeT110I CYCGRG 1 cut(s) 1006
BmgT120I GGNCC 1 cut(s) 871
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 870
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 1 cut(s) 567
BmuI ACTGGG 1 cut(s) 361
BoxI GACNNNNGTC 1 cut(s) 320
BpiI GAAGAC 1 cut(s) 880
BplI GAGNNNNNCTC 2 cut(s) 829, 861
BpmI CTGGAG 2 cut(s) 339, 573
Bpu10I CCTNAGC 2 cut(s) 284, 334
BpuEI CTTGAG 3 cut(s) 55, 860, 1056
BpuMI CCSGG 1 cut(s) 870
Bsa29I ATCGAT 1 cut(s) 430
BsaHI GRCGYC 1 cut(s) 810
BsaI GGTCTC 1 cut(s) 66
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 622, 737
Bse118I RCCGGY 1 cut(s) 1094
Bse1I ACTGG 4 cut(s) 208, 322, 367, 1042
Bse3DI GCAATG 2 cut(s) 778, 1036
BseCI ATCGAT 1 cut(s) 430
BseGI GGATG 3 cut(s) 558, 1060, 1063
BseLI CCNNNNNNNGG 3 cut(s) 173, 622, 737
BseMI GCAATG 2 cut(s) 778, 1036
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 4 cut(s) 208, 322, 367, 1042
BseXI GCAGC 3 cut(s) 325, 764, 1063
BseYI CCCAGC 1 cut(s) 471
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 3 cut(s) 158, 873, 1094
BshVI ATCGAT 1 cut(s) 430
BsiHKAI GWGCWC 1 cut(s) 842
BsiHKCI CYCGRG 1 cut(s) 1006
BsiSI CCGG 2 cut(s) 869, 1095
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 3 cut(s) 173, 622, 737
BsmAI GTCTC 4 cut(s) 66, 174, 801, 949
BsmBI CGTCTC 2 cut(s) 801, 949
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 3 cut(s) 158, 873, 1094
Bso31I GGTCTC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 1006
Bsp1286I GDGCHC 1 cut(s) 842
Bsp143I GATC 3 cut(s) 637, 662, 1099
BspACI CCGC 2 cut(s) 300, 731
BspANI GGCC 3 cut(s) 158, 873, 1094
BspCNI CTCAG 1 cut(s) 347
BspDI ATCGAT 1 cut(s) 430
BspLI GGNNCC 1 cut(s) 31
BspPI GGATC 1 cut(s) 657
BspTNI GGTCTC 1 cut(s) 66
BsrDI GCAATG 2 cut(s) 778, 1036
BsrFI RCCGGY 1 cut(s) 1094
BsrI ACTGG 4 cut(s) 208, 322, 367, 1042
BssAI RCCGGY 1 cut(s) 1094
BssMI GATC 3 cut(s) 637, 662, 1099
BssNI GRCGYC 1 cut(s) 810
Bst4CI ACNGT 4 cut(s) 61, 148, 448, 625
Bst6I CTCTTC 2 cut(s) 172, 416
BstACI GRCGYC 1 cut(s) 810
BstC8I GCNNGC 4 cut(s) 228, 298, 750, 964
BstDEI CTNAG 3 cut(s) 284, 334, 569
BstF5I GGATG 3 cut(s) 558, 1060, 1063
BstKTI GATC 3 cut(s) 640, 665, 1102
BstMAI GTCTC 4 cut(s) 66, 174, 801, 949
BstMBI GATC 3 cut(s) 637, 662, 1099
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNSI RCATGY 1 cut(s) 1026
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 868
BstSFI CTRYAG 2 cut(s) 111, 1014
BstV1I GCAGC 3 cut(s) 325, 764, 1063
BstV2I GAAGAC 1 cut(s) 880
BstX2I RGATCY 1 cut(s) 662
BstXI CCANNNNNNTGG 1 cut(s) 203
BstYI RGATCY 1 cut(s) 662
Bsu15I ATCGAT 1 cut(s) 430
BsuRI GGCC 3 cut(s) 158, 873, 1094
BsuTUI ATCGAT 1 cut(s) 430
BtsCI GGATG 3 cut(s) 558, 1060, 1063
BtsI GCAGTG 2 cut(s) 240, 589
BtsIMutI CAGTG 3 cut(s) 240, 464, 589
Cac8I GCNNGC 4 cut(s) 228, 298, 750, 964
CaiI CAGNNNCTG 2 cut(s) 478, 907
Cfr10I RCCGGY 1 cut(s) 1094
Cfr13I GGNCC 1 cut(s) 871
ClaI ATCGAT 1 cut(s) 430
CseI GACGC 2 cut(s) 778, 818
CviAII CATG 2 cut(s) 946, 1023
DdeI CTNAG 3 cut(s) 284, 334, 569
DpnI GATC 3 cut(s) 639, 664, 1101
DpnII GATC 3 cut(s) 637, 662, 1099
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 1092
Eam1104I CTCTTC 2 cut(s) 172, 416
EarI CTCTTC 2 cut(s) 172, 416
Eco31I GGTCTC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 895
Eco57I CTGAAG 1 cut(s) 1091
Eco88I CYCGRG 1 cut(s) 1006
EcoRV GATATC 1 cut(s) 895
Esp3I CGTCTC 2 cut(s) 801, 949
FaeI CATG 2 cut(s) 949, 1026
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 296, 328
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 945, 1022
FauI CCCGC 1 cut(s) 293
Fnu4HI GCNGC 3 cut(s) 339, 753, 1077
FokI GGATG 3 cut(s) 545, 1050, 1067
Fsp4HI GCNGC 3 cut(s) 339, 753, 1077
FspBI CTAG 2 cut(s) 50, 930
GluI GCNGC 3 cut(s) 339, 753, 1077
GsaI CCCAGC 1 cut(s) 475
GsuI CTGGAG 2 cut(s) 339, 573
HaeIII GGCC 3 cut(s) 158, 873, 1094
HapII CCGG 2 cut(s) 869, 1095
HgaI GACGC 2 cut(s) 778, 818
Hin1I GRCGYC 1 cut(s) 810
Hin1II CATG 2 cut(s) 949, 1026
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 2 cut(s) 224, 878
HinfI GANTC 9 cut(s) 107, 190, 210, 258, 321, 514, 717, 950, 1059
HpaII CCGG 2 cut(s) 869, 1095
HphI GGTGA 4 cut(s) 185, 523, 751, 1109
Hpy166II GTNNAC 3 cut(s) 20, 325, 383
Hpy188I TCNGA 3 cut(s) 597, 649, 684
Hpy188III TCNNGA 5 cut(s) 313, 345, 552, 954, 1131
Hpy8I GTNNAC 3 cut(s) 20, 325, 383
HpyAV CCTTC 2 cut(s) 575, 702
HpyCH4III ACNGT 4 cut(s) 61, 148, 448, 625
HpyCH4V TGCA 3 cut(s) 575, 856, 1002
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 3 cut(s) 284, 334, 569
Hsp92I GRCGYC 1 cut(s) 810
Hsp92II CATG 2 cut(s) 949, 1026
Kzo9I GATC 3 cut(s) 637, 662, 1099
LmnI GCTCC 1 cut(s) 701
Lsp1109I GCAGC 3 cut(s) 325, 764, 1063
LweI GCATC 1 cut(s) 567
MaeI CTAG 2 cut(s) 50, 930
MaeIII GTNAC 4 cut(s) 718, 790, 919, 1033
MalI GATC 3 cut(s) 639, 664, 1101
MboI GATC 3 cut(s) 637, 662, 1099
MboII GAAGA 7 cut(s) 189, 219, 316, 403, 406, 880, 889
MflI RGATCY 1 cut(s) 662
MhlI GDGCHC 1 cut(s) 842
MluCI AATT 4 cut(s) 400, 454, 641, 685
MlyI GAGTC 4 cut(s) 101, 330, 726, 959
MseI TTAA 1 cut(s) 395
MspA1I CMGCKG 2 cut(s) 300, 338
MspI CCGG 2 cut(s) 869, 1095
MspR9I CCNGG 1 cut(s) 870
MwoI GCNNNNNNNGC 1 cut(s) 338
NciI CCSGG 1 cut(s) 870
NdeII GATC 3 cut(s) 637, 662, 1099
NlaIII CATG 2 cut(s) 949, 1026
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 4 cut(s) 718, 790, 919, 1033
NspI RCATGY 1 cut(s) 1026
PaeR7I CTCGAG 1 cut(s) 1006
PciI ACATGT 1 cut(s) 1022
PfeI GAWTC 5 cut(s) 190, 210, 258, 514, 1059
PkrI GCNGC 3 cut(s) 340, 754, 1078
PleI GAGTC 4 cut(s) 101, 329, 725, 958
PpsI GAGTC 4 cut(s) 101, 329, 725, 958
PscI ACATGT 1 cut(s) 1022
PshAI GACNNNNGTC 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 871
PspXI VCTCGAGB 1 cut(s) 1006
PstNI CAGNNNCTG 2 cut(s) 478, 907
PsuI RGATCY 1 cut(s) 662
PvuII CAGCTG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 3 cut(s) 339, 753, 1077
Sau3AI GATC 3 cut(s) 637, 662, 1099
Sau96I GGNCC 1 cut(s) 871
SchI GAGTC 4 cut(s) 101, 330, 726, 959
ScrFI CCNGG 1 cut(s) 870
SduI GDGCHC 1 cut(s) 842
SfaNI GCATC 1 cut(s) 567
SfcI CTRYAG 2 cut(s) 111, 1014
Sfr274I CTCGAG 1 cut(s) 1006
SlaI CTCGAG 1 cut(s) 1006
SmlI CTYRAG 4 cut(s) 34, 875, 1006, 1071
SmoI CTYRAG 4 cut(s) 34, 875, 1006, 1071
Sse9I AATT 4 cut(s) 400, 454, 641, 685
SsiI CCGC 2 cut(s) 300, 731
SspMI CTAG 2 cut(s) 50, 930
StyD4I CCNGG 1 cut(s) 868
TaaI ACNGT 4 cut(s) 61, 148, 448, 625
TaqI TCGA 6 cut(s) 117, 430, 458, 891, 953, 1007
TasI AATT 4 cut(s) 400, 454, 641, 685
TfiI GAWTC 5 cut(s) 190, 210, 258, 514, 1059
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 3 cut(s) 247, 471, 589
TseFI GTSAC 4 cut(s) 718, 790, 919, 1033
TseI GCWGC 3 cut(s) 338, 752, 1076
Tsp45I GTSAC 4 cut(s) 718, 790, 919, 1033
TspDTI ATGAA 6 cut(s) 255, 406, 580, 667, 807, 934
TspGWI ACGGA 2 cut(s) 189, 819
TspRI CASTG 3 cut(s) 247, 471, 589
XapI RAATTY 2 cut(s) 454, 685
XceI RCATGY 1 cut(s) 1026
XcmI CCANNNNNNNNNTGG 1 cut(s) 1087
XhoI CTCGAG 1 cut(s) 1006
XspI CTAG 2 cut(s) 50, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.