Rh3AG168300

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0006535
Physical Location & Seq
Forward (+)
15580704 .. 15582485
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG168300.1

Sequence Viewer

Length: 960 bp
ATGATAGTTTCCGGTAAACTCTTGGTTTCCTTGAGCCTTCTAGTTCTTGTTTTCAAAGTGTCTCATGGGCAGCCTTTAGTTCCTGCACTCATCATCTTTGGGGACTCTGTTGCCGATGTGGGAAATAATAACAACCGCACTACACTAGTGAAGGCAAACTTCCCTCCTTATGGAAGAGACTTCGTTAATCATCAACCGACTGGAAGATTTTGTAATGGGAAGCTTGCCACAGACTTCACCGCTGAATATCTCGGATTCACCTCATACCCACCAGCTTTCCTTAGCCAAGAAGCCACAGGGATGAGCATTTTGACTGGAGTCAACTTTGCCTCAGCTGCTTCTGGCTATTATAACTGGACAGCCCAGTTATGGGGTGCAATTTCACTAGACCAACAATTAAATTACTACAATGAGTATCAAAGTGTAGTGGTGAATATGGTAGGGGAAGCCGAAGCCAATGCCATATTCTCTGGCGCCATCCACCTTCTAAGTGCAGGAACCAGCGATTTCATTCAGAACTACTACATCAATCCATTTCTTAGAGTCTACACACCTGACCAATTCTCAGAAATTCTAATGAGATCCTACTCCTCTTTCATTCAGAAGTTGTATGGACTCGGAGCACGAAGGATTGGAGTCACAAACTTACCACCTATAGGATGTTTGCCAGCAGCAATTACCCTATTTGGCTCAGGAAGCAACCAGTGGAACATAAGATTGAATCAAGATGCCATTTTATTCAATAACAAGCTTAACAACACATCTCAAACTTTGCAGAAGAGTCTTCCCGGACTCAAGCTCGTAGTTTTTGATATCTACCAGCCCCTCCTGGATATGATCACAAACCCCAGTGACAATGGTAAAACACCTCTGTGTGTAGAAGTTAATCAATGCAACAAAAAGAGAAAACCAATGGTTCTTCGAGTCAAGAAGAGCTTGTTGTGGAACAGGTACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

319

Amino Acids

35.33

Weight (kDa)

9.16

Isoelectric Point (pI)

27.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 275 5e-24 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 546
AciI CCGC 2 cut(s) 136, 240
AclWI GGATC 1 cut(s) 576
AcsI RAATTY 1 cut(s) 570
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 1 cut(s) 953
AfiI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
AgsI TTSAA 3 cut(s) 55, 721, 742
AhlI ACTAGT 1 cut(s) 145
AjnI CCWGG 1 cut(s) 828
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AleI CACNNNNGTG 1 cut(s) 871
AluBI AGCT 6 cut(s) 223, 275, 335, 751, 799, 936
AluI AGCT 6 cut(s) 223, 275, 335, 751, 799, 936
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 2 cut(s) 66, 171
AlwI GGATC 1 cut(s) 576
ApeKI GCWGC 3 cut(s) 70, 335, 671
ApoI RAATTY 1 cut(s) 570
AspLEI GCGC 1 cut(s) 476
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 3 cut(s) 229, 250, 442
BanI GGYRCC 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 1 cut(s) 625
BbvCI CCTCAGC 1 cut(s) 331
BbvI GCAGC 3 cut(s) 82, 322, 683
BccI CCATC 1 cut(s) 485
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 1 cut(s) 830
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 66, 171
BcuI ACTAGT 1 cut(s) 145
BfaI CTAG 3 cut(s) 41, 146, 386
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 477
BisI GCNGC 3 cut(s) 71, 336, 672
BlsI GCNGC 3 cut(s) 72, 337, 673
Bme1390I CCNGG 2 cut(s) 789, 830
BmiI GGNNCC 2 cut(s) 475, 499
BmrFI CCNGG 2 cut(s) 789, 830
BmrI ACTGGG 2 cut(s) 358, 843
BmsI GCATC 1 cut(s) 718
BmuI ACTGGG 2 cut(s) 358, 843
BoxI GACNNNNGTC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 776
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 3 cut(s) 281, 331, 691
BpuEI CTTGAG 2 cut(s) 52, 779
BpuMI CCSGG 1 cut(s) 789
BsaHI GRCGYC 1 cut(s) 474
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
Bse1I ACTGG 6 cut(s) 205, 319, 359, 364, 703, 849
BseBI CCWGG 1 cut(s) 830
BseGI GGATG 3 cut(s) 306, 477, 665
BseLI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
BseMII CTCAG 3 cut(s) 345, 579, 705
BseNI ACTGG 6 cut(s) 205, 319, 359, 364, 703, 849
BseRI GAGGAG 1 cut(s) 580
BseXI GCAGC 3 cut(s) 82, 322, 683
BsgI GTGCAG 2 cut(s) 69, 513
BshNI GGYRCC 1 cut(s) 473
BsiHKAI GWGCWC 1 cut(s) 625
BsiSI CCGG 2 cut(s) 12, 789
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
BsmAI GTCTC 2 cut(s) 66, 171
BsmFI GGGAC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 625
Bsp143I GATC 2 cut(s) 581, 837
BspACI CCGC 2 cut(s) 136, 240
BspCNI CTCAG 3 cut(s) 344, 578, 704
BspLI GGNNCC 2 cut(s) 475, 499
BspPI GGATC 1 cut(s) 576
BspQI GCTCTTC 1 cut(s) 926
BspT107I GGYRCC 1 cut(s) 473
BsrI ACTGG 6 cut(s) 205, 319, 359, 364, 703, 849
BssMI GATC 2 cut(s) 581, 837
BssNI GRCGYC 1 cut(s) 474
Bst2UI CCWGG 1 cut(s) 830
Bst6I CTCTTC 3 cut(s) 169, 773, 926
BstACI GRCGYC 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 225, 669
BstDEI CTNAG 6 cut(s) 281, 331, 488, 539, 565, 691
BstF5I GGATG 3 cut(s) 306, 477, 665
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 2 cut(s) 584, 840
BstMAI GTCTC 2 cut(s) 66, 171
BstMBI GATC 2 cut(s) 581, 837
BstMWI GCNNNNNNNGC 2 cut(s) 335, 696
BstNI CCWGG 1 cut(s) 830
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 787, 828
BstSFI CTRYAG 1 cut(s) 654
BstV1I GCAGC 3 cut(s) 82, 322, 683
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
BtsCI GGATG 3 cut(s) 306, 477, 665
BtsIMutI CAGTG 2 cut(s) 710, 856
Cac8I GCNNGC 2 cut(s) 225, 669
CfoI GCGC 1 cut(s) 476
Csp6I GTAC 1 cut(s) 952
CviAII CATG 1 cut(s) 65
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 6 cut(s) 281, 331, 488, 539, 565, 691
DinI GGCGCC 1 cut(s) 475
DpnI GATC 2 cut(s) 583, 839
DpnII GATC 2 cut(s) 581, 837
Eam1104I CTCTTC 3 cut(s) 169, 773, 926
EarI CTCTTC 3 cut(s) 169, 773, 926
Eco32I GATATC 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 828
EcoRV GATATC 1 cut(s) 814
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
FaeI CATG 1 cut(s) 68
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 920, 952
FaqI GGGAC 1 cut(s) 116
FatI CATG 1 cut(s) 64
FbaI TGATCA 1 cut(s) 837
FblI GTMKAC 1 cut(s) 546
Fnu4HI GCNGC 3 cut(s) 71, 336, 672
FokI GGATG 3 cut(s) 313, 464, 672
Fsp4HI GCNGC 3 cut(s) 71, 336, 672
FspBI CTAG 3 cut(s) 41, 146, 386
GlaI GCGC 1 cut(s) 475
GluI GCNGC 3 cut(s) 71, 336, 672
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 477
HapII CCGG 2 cut(s) 12, 789
HhaI GCGC 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 1 cut(s) 68
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 2 cut(s) 221, 749
HpaII CCGG 2 cut(s) 12, 789
HphI GGTGA 3 cut(s) 229, 250, 442
Hpy166II GTNNAC 3 cut(s) 17, 322, 547
Hpy188I TCNGA 5 cut(s) 254, 516, 568, 603, 620
Hpy188III TCNNGA 3 cut(s) 693, 725, 928
Hpy8I GTNNAC 3 cut(s) 17, 322, 547
HpyAV CCTTC 4 cut(s) 47, 145, 494, 621
HpyCH4V TGCA 5 cut(s) 86, 377, 494, 775, 894
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 696
HpyF3I CTNAG 6 cut(s) 281, 331, 488, 539, 565, 691
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 1 cut(s) 68
HspAI GCGC 1 cut(s) 474
KasI GGCGCC 1 cut(s) 473
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 2 cut(s) 581, 837
LguI GCTCTTC 1 cut(s) 926
LmnI GCTCC 1 cut(s) 620
Lsp1109I GCAGC 3 cut(s) 82, 322, 683
LweI GCATC 1 cut(s) 718
MaeI CTAG 3 cut(s) 41, 146, 386
MaeIII GTNAC 2 cut(s) 637, 851
MalI GATC 2 cut(s) 583, 839
MboI GATC 2 cut(s) 581, 837
MboII GAAGA 6 cut(s) 186, 216, 776, 790, 911, 943
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 1 cut(s) 625
MluCI AATT 6 cut(s) 378, 395, 400, 560, 570, 675
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 8 cut(s) 98, 327, 552, 609, 645, 786, 790, 933
MnlI CCTC 6 cut(s) 174, 271, 340, 601, 836, 879
MseI TTAA 4 cut(s) 186, 398, 753, 885
MslI CAYNNNNRTG 2 cut(s) 299, 871
MspA1I CMGCKG 2 cut(s) 242, 335
MspI CCGG 2 cut(s) 12, 789
MspR9I CCNGG 2 cut(s) 789, 830
MvaI CCWGG 1 cut(s) 830
MwoI GCNNNNNNNGC 2 cut(s) 335, 696
NarI GGCGCC 1 cut(s) 474
NciI CCSGG 1 cut(s) 789
NdeII GATC 2 cut(s) 581, 837
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 2 cut(s) 475, 499
NmuCI GTSAC 2 cut(s) 637, 851
OliI CACNNNNGTG 1 cut(s) 871
PciSI GCTCTTC 1 cut(s) 926
PfeI GAWTC 2 cut(s) 255, 721
PfoI TCCNGGA 2 cut(s) 787, 828
PkrI GCNGC 3 cut(s) 72, 337, 673
PleI GAGTC 8 cut(s) 98, 326, 551, 609, 644, 786, 789, 932
PluTI GGCGCC 1 cut(s) 477
PpsI GAGTC 8 cut(s) 98, 326, 551, 609, 644, 786, 789, 932
PshAI GACNNNNGTC 1 cut(s) 317
PsiI TTATAA 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 828
PspGI CCWGG 1 cut(s) 828
PspN4I GGNNCC 2 cut(s) 475, 499
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
RseI CAYNNNNRTG 2 cut(s) 299, 871
SapI GCTCTTC 1 cut(s) 926
SaqAI TTAA 4 cut(s) 186, 398, 753, 885
SatI GCNGC 3 cut(s) 71, 336, 672
Sau3AI GATC 2 cut(s) 581, 837
SchI GAGTC 8 cut(s) 98, 327, 552, 609, 645, 786, 790, 933
ScrFI CCNGG 2 cut(s) 789, 830
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 1 cut(s) 718
SfcI CTRYAG 1 cut(s) 654
SfoI GGCGCC 1 cut(s) 475
SmiMI CAYNNNNRTG 2 cut(s) 299, 871
SmlI CTYRAG 2 cut(s) 31, 794
SmoI CTYRAG 2 cut(s) 31, 794
SpeI ACTAGT 1 cut(s) 145
Sse9I AATT 6 cut(s) 378, 395, 400, 560, 570, 675
SsiI CCGC 2 cut(s) 136, 240
SspDI GGCGCC 1 cut(s) 473
SspMI CTAG 3 cut(s) 41, 146, 386
StyD4I CCNGG 2 cut(s) 787, 828
TaqI TCGA 1 cut(s) 922
TasI AATT 6 cut(s) 378, 395, 400, 560, 570, 675
TfiI GAWTC 2 cut(s) 255, 721
Tru1I TTAA 4 cut(s) 186, 398, 753, 885
Tru9I TTAA 4 cut(s) 186, 398, 753, 885
TscAI CASTG 2 cut(s) 710, 856
TseFI GTSAC 2 cut(s) 637, 851
TseI GCWGC 3 cut(s) 70, 335, 671
Tsp45I GTSAC 2 cut(s) 637, 851
TspDTI ATGAA 2 cut(s) 499, 586
TspRI CASTG 2 cut(s) 710, 856
XapI RAATTY 1 cut(s) 570
XmiI GTMKAC 1 cut(s) 546
XspI CTAG 3 cut(s) 41, 146, 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.