RLG00000024170

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0006535
Physical Location & Seq
Reverse (-)
32737577 .. 32739308
1732 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024170

Sequence Viewer

Length: 1053 bp
ATGATAGTTTCCGGTAAACTCTTGGTTTCCTTGAGCCTTCTAGTTCTTGTTTTCAAAGTGTCTCATGGGCAGCCTTTAGTTCCTGCACTTATCATCTTTGGGGACTCTGTTGCTGATGTGGGAAATAATAACAACCGCACTACACTAGTGAAGGCAAACTTCCCTCCTTATGGAAGAGACTTCGTTAATCATCAACCGACTGGAAGATTTTGTAATGGGAAGCTTGCCACAGACTTCACCGCTGAATATCTCGGATTCACCTCATACCCACCAGCTTTCCTTGGCCAAGAAGCCACAGGGATGAGCATTTTGACTGGAGTCAACTTTGCCTCAGCTGCTTCTGGCTATTATAACTGGACAGCCCAGTTATGGGGTGCAATTTCACTAGACCAACAATTAAATTATTACAATGAGTATCAAAGTATAGTGGTGAATATGGTAGGGGAAGCCGAAGCCAATGCCATATTCTCTGGCGCCATCCACCTTCTAAGTGCAGGAACCAGCGATTTCATTCAGAACTACTACATCAACCCATTTCTTAGAGTCTACACCCCTGACCAATTCTCAGAAATTCTAATGAGATCCTACTCCTCTTTCATTCAGAAGTTGTATGGACTTGGAGCACGAAGGATTGGAGTCACAAACTTACCACCTATAGGATGTTTGCCAGCAGCAATTACCCTATTTGGTTCAGGAAGCAACCAGTGGAACATAAGATTGAATCAAGATGCCATTTTATTCAATAACAAGCTTAACAACACATCTCAAACTTTGCAGAGGAGTCTTCCCGGACTCAAGCTCGTAGTTTTTGATATCTACCAGCCCCTCCTGGATATGATCACAAACCCCAGTGACAATGGGTTCTTCGAGTCAAGAAGAGCTTGTTGTGGAACAGGTACATTTGAAACATCTGTGCTCTGCAACTCTAGGTCTGTAGGAACATGCAAAAATGCAACTGGTTATGTGTTCTGGGATGGATTTCATCCCTCTGAAGCTGCCAATGAGGTCTTAGCTGGTGATCTACTTGCACAGGGCATCGATCTCATATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

38.18

Weight (kDa)

5.56

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 338 1.9e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 546
AciI CCGC 2 cut(s) 136, 240
AclWI GGATC 1 cut(s) 576
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 1011
AcyI GRCGYC 1 cut(s) 474
AfaI GTAC 1 cut(s) 898
AfiI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
AgsI TTSAA 4 cut(s) 55, 721, 742, 905
AhlI ACTAGT 1 cut(s) 145
AjnI CCWGG 1 cut(s) 828
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AluBI AGCT 8 cut(s) 223, 275, 335, 751, 799, 881, 995, 1013
AluI AGCT 8 cut(s) 223, 275, 335, 751, 799, 881, 995, 1013
Alw21I GWGCWC 2 cut(s) 625, 918
Alw26I GTCTC 2 cut(s) 66, 171
AlwI GGATC 1 cut(s) 576
AoxI GGCC 1 cut(s) 283
ApeKI GCWGC 4 cut(s) 70, 335, 671, 995
ApoI RAATTY 1 cut(s) 570
AspLEI GCGC 1 cut(s) 476
AsuC2I CCSGG 1 cut(s) 789
AsuHPI GGTGA 4 cut(s) 229, 250, 442, 1028
BalI TGGCCA 1 cut(s) 285
BanI GGYRCC 1 cut(s) 473
BbsI GAAGAC 1 cut(s) 776
Bbv12I GWGCWC 2 cut(s) 625, 918
BbvCI CCTCAGC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 82, 322, 683, 982
BccI CCATC 2 cut(s) 485, 968
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 1 cut(s) 830
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 1 cut(s) 789
BcoDI GTCTC 2 cut(s) 66, 171
BcuI ACTAGT 1 cut(s) 145
BfaI CTAG 4 cut(s) 41, 146, 386, 927
BfmI CTRYAG 2 cut(s) 654, 933
BfoI RGCGCY 1 cut(s) 477
BisI GCNGC 4 cut(s) 71, 336, 672, 996
BlsI GCNGC 4 cut(s) 72, 337, 673, 997
Bme1390I CCNGG 2 cut(s) 789, 830
BmiI GGNNCC 2 cut(s) 475, 499
BmrFI CCNGG 2 cut(s) 789, 830
BmrI ACTGGG 2 cut(s) 358, 843
BmsI GCATC 2 cut(s) 718, 1044
BmuI ACTGGG 2 cut(s) 358, 843
BoxI GACNNNNGTC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 776
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 331
BpuEI CTTGAG 2 cut(s) 52, 779
BpuMI CCSGG 1 cut(s) 789
Bsa29I ATCGAT 1 cut(s) 1038
BsaHI GRCGYC 1 cut(s) 474
BsaJI CCNNGG 1 cut(s) 280
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
Bse1I ACTGG 7 cut(s) 205, 319, 359, 364, 703, 849, 961
BseBI CCWGG 1 cut(s) 830
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 1 cut(s) 280
BseGI GGATG 5 cut(s) 306, 477, 665, 979, 982
BseLI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
BseMII CTCAG 2 cut(s) 345, 579
BseNI ACTGG 7 cut(s) 205, 319, 359, 364, 703, 849, 961
BseRI GAGGAG 2 cut(s) 580, 793
BseXI GCAGC 4 cut(s) 82, 322, 683, 982
BsgI GTGCAG 2 cut(s) 69, 513
BshFI GGCC 1 cut(s) 285
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 1038
BsiHKAI GWGCWC 2 cut(s) 625, 918
BsiSI CCGG 2 cut(s) 12, 789
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 4 cut(s) 170, 369, 370, 656
BsmAI GTCTC 2 cut(s) 66, 171
BsmFI GGGAC 1 cut(s) 116
BsnI GGCC 1 cut(s) 285
Bsp1286I GDGCHC 2 cut(s) 625, 918
Bsp143I GATC 4 cut(s) 581, 837, 1018, 1039
BspACI CCGC 2 cut(s) 136, 240
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 2 cut(s) 344, 578
BspDI ATCGAT 1 cut(s) 1038
BspLI GGNNCC 2 cut(s) 475, 499
BspPI GGATC 1 cut(s) 576
BspQI GCTCTTC 1 cut(s) 871
BspT107I GGYRCC 1 cut(s) 473
BsrI ACTGG 7 cut(s) 205, 319, 359, 364, 703, 849, 961
BssECI CCNNGG 1 cut(s) 280
BssMI GATC 4 cut(s) 581, 837, 1018, 1039
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 1 cut(s) 280
Bst2UI CCWGG 1 cut(s) 830
Bst6I CTCTTC 2 cut(s) 169, 871
BstACI GRCGYC 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 225, 669
BstDEI CTNAG 5 cut(s) 331, 488, 539, 565, 1009
BstF5I GGATG 5 cut(s) 306, 477, 665, 979, 982
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 4 cut(s) 584, 840, 1021, 1042
BstMAI GTCTC 2 cut(s) 66, 171
BstMBI GATC 4 cut(s) 581, 837, 1018, 1039
BstMWI GCNNNNNNNGC 1 cut(s) 335
BstNI CCWGG 1 cut(s) 830
BstNSI RCATGY 1 cut(s) 945
BstPAI GACNNNNGTC 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 787, 828
BstSFI CTRYAG 2 cut(s) 654, 933
BstV1I GCAGC 4 cut(s) 82, 322, 683, 982
BstV2I GAAGAC 1 cut(s) 776
BstX2I RGATCY 1 cut(s) 581
BstYI RGATCY 1 cut(s) 581
Bsu15I ATCGAT 1 cut(s) 1038
BsuRI GGCC 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 1038
BtsCI GGATG 5 cut(s) 306, 477, 665, 979, 982
BtsIMutI CAGTG 2 cut(s) 710, 856
Cac8I GCNNGC 2 cut(s) 225, 669
CfoI GCGC 1 cut(s) 476
ClaI ATCGAT 1 cut(s) 1038
Csp6I GTAC 1 cut(s) 897
CviAII CATG 2 cut(s) 65, 942
CviQI GTAC 1 cut(s) 897
DdeI CTNAG 5 cut(s) 331, 488, 539, 565, 1009
DinI GGCGCC 1 cut(s) 475
DpnI GATC 4 cut(s) 583, 839, 1020, 1041
DpnII GATC 4 cut(s) 581, 837, 1018, 1039
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 169, 871
EarI CTCTTC 2 cut(s) 169, 871
Eco130I CCWWGG 1 cut(s) 280
Eco32I GATATC 1 cut(s) 814
Eco57I CTGAAG 1 cut(s) 1011
EcoRII CCWGG 1 cut(s) 828
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 1 cut(s) 280
EgeI GGCGCC 1 cut(s) 475
EheI GGCGCC 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 280
FaeI CATG 2 cut(s) 68, 945
FalI AAGNNNNNCTT 4 cut(s) 143, 175, 865, 897
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 64, 941
FbaI TGATCA 1 cut(s) 837
FblI GTMKAC 1 cut(s) 546
Fnu4HI GCNGC 4 cut(s) 71, 336, 672, 996
FokI GGATG 5 cut(s) 313, 464, 672, 969, 986
Fsp4HI GCNGC 4 cut(s) 71, 336, 672, 996
FspBI CTAG 4 cut(s) 41, 146, 386, 927
GlaI GCGC 1 cut(s) 475
GluI GCNGC 4 cut(s) 71, 336, 672, 996
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 1 cut(s) 285
HapII CCGG 2 cut(s) 12, 789
HhaI GCGC 1 cut(s) 476
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 2 cut(s) 68, 945
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 2 cut(s) 221, 749
HinfI GANTC 9 cut(s) 104, 255, 318, 543, 636, 721, 781, 792, 869
HpaII CCGG 2 cut(s) 12, 789
HphI GGTGA 4 cut(s) 229, 250, 442, 1028
Hpy166II GTNNAC 3 cut(s) 17, 322, 547
Hpy188I TCNGA 5 cut(s) 254, 516, 568, 603, 991
Hpy188III TCNNGA 4 cut(s) 693, 725, 873, 1050
Hpy8I GTNNAC 3 cut(s) 17, 322, 547
HpyAV CCTTC 4 cut(s) 47, 145, 494, 621
HpyCH4V TGCA 8 cut(s) 86, 377, 494, 775, 921, 945, 953, 1028
HpyF10VI GCNNNNNNNGC 1 cut(s) 335
HpyF3I CTNAG 5 cut(s) 331, 488, 539, 565, 1009
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 2 cut(s) 68, 945
HspAI GCGC 1 cut(s) 474
KasI GGCGCC 1 cut(s) 473
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 4 cut(s) 581, 837, 1018, 1039
LguI GCTCTTC 1 cut(s) 871
LmnI GCTCC 1 cut(s) 620
Lsp1109I GCAGC 4 cut(s) 82, 322, 683, 982
LweI GCATC 2 cut(s) 718, 1044
MaeI CTAG 4 cut(s) 41, 146, 386, 927
MaeIII GTNAC 2 cut(s) 637, 851
MalI GATC 4 cut(s) 583, 839, 1020, 1041
MboI GATC 4 cut(s) 581, 837, 1018, 1039
MboII GAAGA 5 cut(s) 186, 216, 776, 856, 888
MflI RGATCY 1 cut(s) 581
MhlI GDGCHC 2 cut(s) 625, 918
MlsI TGGCCA 1 cut(s) 285
MluCI AATT 6 cut(s) 378, 395, 400, 560, 570, 675
MluNI TGGCCA 1 cut(s) 285
Mly113I GGCGCC 1 cut(s) 474
MlyI GAGTC 7 cut(s) 98, 327, 552, 645, 786, 790, 878
MnlI CCTC 8 cut(s) 174, 271, 340, 601, 771, 836, 997, 997
Mox20I TGGCCA 1 cut(s) 285
MscI TGGCCA 1 cut(s) 285
MseI TTAA 3 cut(s) 186, 398, 753
MslI CAYNNNNRTG 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 242, 335
MspI CCGG 2 cut(s) 12, 789
MspR9I CCNGG 2 cut(s) 789, 830
MvaI CCWGG 1 cut(s) 830
MwoI GCNNNNNNNGC 1 cut(s) 335
NarI GGCGCC 1 cut(s) 474
NciI CCSGG 1 cut(s) 789
NdeII GATC 4 cut(s) 581, 837, 1018, 1039
NlaIII CATG 2 cut(s) 68, 945
NlaIV GGNNCC 2 cut(s) 475, 499
NmuCI GTSAC 2 cut(s) 637, 851
NspI RCATGY 1 cut(s) 945
PciSI GCTCTTC 1 cut(s) 871
PfeI GAWTC 2 cut(s) 255, 721
PfoI TCCNGGA 2 cut(s) 787, 828
PkrI GCNGC 4 cut(s) 72, 337, 673, 997
PleI GAGTC 7 cut(s) 98, 326, 551, 644, 786, 789, 877
PluTI GGCGCC 1 cut(s) 477
PpsI GAGTC 7 cut(s) 98, 326, 551, 644, 786, 789, 877
PshAI GACNNNNGTC 1 cut(s) 317
PsiI TTATAA 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 828
PspGI CCWGG 1 cut(s) 828
PspN4I GGNNCC 2 cut(s) 475, 499
PsuI RGATCY 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 898
RsaNI GTAC 1 cut(s) 897
RseI CAYNNNNRTG 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 871
SaqAI TTAA 3 cut(s) 186, 398, 753
SatI GCNGC 4 cut(s) 71, 336, 672, 996
Sau3AI GATC 4 cut(s) 581, 837, 1018, 1039
SchI GAGTC 7 cut(s) 98, 327, 552, 645, 786, 790, 878
ScrFI CCNGG 2 cut(s) 789, 830
SduI GDGCHC 2 cut(s) 625, 918
SfaNI GCATC 2 cut(s) 718, 1044
SfcI CTRYAG 2 cut(s) 654, 933
SfoI GGCGCC 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 2 cut(s) 31, 794
SmoI CTYRAG 2 cut(s) 31, 794
SpeI ACTAGT 1 cut(s) 145
Sse9I AATT 6 cut(s) 378, 395, 400, 560, 570, 675
SsiI CCGC 2 cut(s) 136, 240
SspDI GGCGCC 1 cut(s) 473
SspMI CTAG 4 cut(s) 41, 146, 386, 927
StyD4I CCNGG 2 cut(s) 787, 828
StyI CCWWGG 1 cut(s) 280
TaqI TCGA 2 cut(s) 867, 1038
TasI AATT 6 cut(s) 378, 395, 400, 560, 570, 675
TfiI GAWTC 2 cut(s) 255, 721
Tru1I TTAA 3 cut(s) 186, 398, 753
Tru9I TTAA 3 cut(s) 186, 398, 753
TscAI CASTG 2 cut(s) 710, 856
TseFI GTSAC 2 cut(s) 637, 851
TseI GCWGC 4 cut(s) 70, 335, 671, 995
Tsp45I GTSAC 2 cut(s) 637, 851
TspDTI ATGAA 3 cut(s) 499, 586, 971
TspRI CASTG 2 cut(s) 710, 856
XapI RAATTY 1 cut(s) 570
XceI RCATGY 1 cut(s) 945
XmiI GTMKAC 1 cut(s) 546
XspI CTAG 4 cut(s) 41, 146, 386, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.