FvH4_6g17360

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006535
Physical Location & Seq
Forward (+)
11279228 .. 11282818
3591 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17360.t1

Sequence Viewer

Length: 1053 bp
ATGGAGTTTTCAAGCAGTAAACTCTTTGGTTCCCTGACCTTTCTAGCTTTGGTTTTCACAGTGGTTCTTGGAGACCCTTTAGTTCCAGCTCTTTTCATCTTTGGGGACTCTGTAGTCGATGTGGGAAACAACAACAATCTCAGCACAGTCATCAAGGCCAACTTCCCTCCTTATGGAAGAGACTTTGTGAATCACAAACCAACTGGAAGATTCTGTAATGGAAAGCTTGCCACCGATTTCACTGCTGAATACCTGGGATTCACTACATATCAACCAGCTTACCTAAGCCAAGAAGCCAGCGGGAAGAACCTCTTGACTGGAGCCAACTTTGCCTCTGCGGCTTCTGGATTTTATGACCCAACCGCCCTGTTATATAATTCGATTTCACTGACCCAGCAACTGAACAACTACAAGGAGTATCAAAGTAAAGTGGTGAATCTGGTAGGACAAACCAAAGCCAATGCCATATTCTCTGGAGGCATCCACCTTCTAAGTGCAGGGAGCAGTGATTTCATTCAGAACTATTACATCACTCCCCTAGCTACGGTCTACACGCCCGATCAATTCTCAGACATCCTCATTAGATCCTACTCTACATTCATTCAGAATTTGTATGGATTGGGAGCAAGAAGGATTGGAGTCACAAGCTTACCGCCAACCGGGTGTTTGCCAGCAGCAATCACCCTTTTTGGCTTAGGAAGCAACAATTGTGTCTCAAGATTAAACGGAGATGCCATTTCATTCAACAATAAGCTAAAGAGCACATCTCAAAGTCTGACGACCAAACTTCCAGGCCTCAAGCTTGTGGTCTTTGATATCTTTCAGCCTCTCCTGGATATGGTCACAAAGCCTAGTGATAATGGGTTCATGGAGTCGAGACGAGCTTGCTGTGGAACAGGAACACTAGAAACCTCTGTGCTTTGCAACTCGAGGTCTATAGGAACATGTAGCAATGTGACCGGGTATGTGTTTTGGGATGGATTCCATCCCTCTCAAGCTGCTAACGAGGTCTTGGCTGGTGATCTCCTTCAGCAAGGTTTGGCTCTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

37.64

Weight (kDa)

6.8

Isoelectric Point (pI)

29.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 31 - 339 2e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 549
AciI CCGC 4 cut(s) 300, 338, 363, 653
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 1 cut(s) 607
AcuI CTGAAG 1 cut(s) 1013
AfiI CCNNNNNNNGG 4 cut(s) 173, 544, 659, 838
AflIII ACRYGT 1 cut(s) 944
AgsI TTSAA 2 cut(s) 12, 745
AjnI CCWGG 3 cut(s) 252, 790, 831
AjuI GAANNNNNNNTTGG 2 cut(s) 452, 484
Alw21I GWGCWC 1 cut(s) 764
Alw26I GTCTC 4 cut(s) 66, 174, 718, 871
AlwI GGATC 1 cut(s) 579
AlwNI CAGNNNCTG 1 cut(s) 400
Ama87I CYCGRG 1 cut(s) 928
AoxI GGCC 2 cut(s) 156, 793
ApeKI GCWGC 2 cut(s) 674, 998
ApoI RAATTY 1 cut(s) 607
AsuC2I CCSGG 2 cut(s) 661, 961
AsuHPI GGTGA 3 cut(s) 445, 673, 1031
AvaI CYCGRG 1 cut(s) 928
Bbv12I GWGCWC 1 cut(s) 764
BbvI GCAGC 2 cut(s) 686, 985
BccI CCATC 2 cut(s) 971, 993
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 3 cut(s) 254, 792, 833
BcnI CCSGG 2 cut(s) 661, 961
BcoDI GTCTC 4 cut(s) 66, 174, 718, 871
BfaI CTAG 4 cut(s) 44, 539, 852, 905
BfmI CTRYAG 2 cut(s) 111, 936
BglI GCCNNNNNGGC 1 cut(s) 338
BisI GCNGC 3 cut(s) 339, 675, 999
BlsI GCNGC 3 cut(s) 340, 676, 1000
Bme1390I CCNGG 5 cut(s) 254, 661, 792, 833, 961
BmeT110I CYCGRG 1 cut(s) 928
BmiI GGNNCC 2 cut(s) 31, 322
BmrFI CCNGG 5 cut(s) 254, 661, 792, 833, 961
BmsI GCATC 2 cut(s) 489, 721
BplI GAGNNNNNCTC 2 cut(s) 751, 783
BpmI CTGGAG 2 cut(s) 339, 495
Bpu10I CCTNAGC 2 cut(s) 284, 694
BpuEI CTTGAG 3 cut(s) 700, 782, 978
BpuMI CCSGG 2 cut(s) 661, 961
BsaI GGTCTC 1 cut(s) 66
BsaJI CCNNGG 1 cut(s) 253
Bsc4I CCNNNNNNNGG 4 cut(s) 173, 544, 659, 838
Bse1I ACTGG 2 cut(s) 208, 322
Bse3DI GCAATG 1 cut(s) 958
BseBI CCWGG 3 cut(s) 254, 792, 833
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 4 cut(s) 480, 573, 982, 985
BseLI CCNNNNNNNGG 4 cut(s) 173, 544, 659, 838
BseMI GCAATG 1 cut(s) 958
BseMII CTCAG 2 cut(s) 154, 582
BseNI ACTGG 2 cut(s) 208, 322
BseXI GCAGC 2 cut(s) 686, 985
BseYI CCCAGC 1 cut(s) 393
BsgI GTGCAG 1 cut(s) 516
BshFI GGCC 2 cut(s) 158, 795
BsiHKAI GWGCWC 1 cut(s) 764
BsiHKCI CYCGRG 1 cut(s) 928
BsiSI CCGG 2 cut(s) 660, 960
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 4 cut(s) 173, 544, 659, 838
BsmAI GTCTC 4 cut(s) 66, 174, 718, 871
BsmBI CGTCTC 1 cut(s) 871
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 2 cut(s) 158, 795
Bso31I GGTCTC 1 cut(s) 66
BsoBI CYCGRG 1 cut(s) 928
Bsp1286I GDGCHC 1 cut(s) 764
Bsp143I GATC 3 cut(s) 559, 584, 1021
BspACI CCGC 4 cut(s) 300, 338, 363, 653
BspANI GGCC 2 cut(s) 158, 795
BspCNI CTCAG 2 cut(s) 153, 581
BspLI GGNNCC 2 cut(s) 31, 322
BspPI GGATC 1 cut(s) 579
BspTNI GGTCTC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 958
BsrI ACTGG 2 cut(s) 208, 322
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 3 cut(s) 559, 584, 1021
Bst2UI CCWGG 3 cut(s) 254, 792, 833
Bst4CI ACNGT 3 cut(s) 61, 148, 547
Bst6I CTCTTC 1 cut(s) 172
BstC8I GCNNGC 4 cut(s) 228, 298, 672, 886
BstDEI CTNAG 5 cut(s) 140, 284, 491, 568, 694
BstF5I GGATG 4 cut(s) 480, 573, 982, 985
BstKTI GATC 3 cut(s) 562, 587, 1024
BstMAI GTCTC 4 cut(s) 66, 174, 718, 871
BstMBI GATC 3 cut(s) 559, 584, 1021
BstMWI GCNNNNNNNGC 3 cut(s) 329, 338, 699
BstNI CCWGG 3 cut(s) 254, 792, 833
BstNSI RCATGY 1 cut(s) 948
BstSCI CCNGG 5 cut(s) 252, 659, 790, 831, 959
BstSFI CTRYAG 2 cut(s) 111, 936
BstV1I GCAGC 2 cut(s) 686, 985
BstX2I RGATCY 1 cut(s) 584
BstYI RGATCY 1 cut(s) 584
BsuRI GGCC 2 cut(s) 158, 795
BtsCI GGATG 4 cut(s) 480, 573, 982, 985
BtsI GCAGTG 2 cut(s) 240, 511
BtsIMutI CAGTG 4 cut(s) 66, 240, 386, 511
Cac8I GCNNGC 4 cut(s) 228, 298, 672, 886
CaiI CAGNNNCTG 1 cut(s) 400
CviAII CATG 2 cut(s) 868, 945
DdeI CTNAG 5 cut(s) 140, 284, 491, 568, 694
DpnI GATC 3 cut(s) 561, 586, 1023
DpnII GATC 3 cut(s) 559, 584, 1021
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
Eam1104I CTCTTC 1 cut(s) 172
EarI CTCTTC 1 cut(s) 172
Eco147I AGGCCT 1 cut(s) 795
Eco31I GGTCTC 1 cut(s) 66
Eco32I GATATC 1 cut(s) 817
Eco57I CTGAAG 1 cut(s) 1013
Eco88I CYCGRG 1 cut(s) 928
EcoRII CCWGG 3 cut(s) 252, 790, 831
EcoRV GATATC 1 cut(s) 817
Esp3I CGTCTC 1 cut(s) 871
FaeI CATG 2 cut(s) 871, 948
FalI AAGNNNNNCTT 4 cut(s) 146, 178, 296, 328
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 867, 944
FauI CCCGC 1 cut(s) 293
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 3 cut(s) 339, 675, 999
FokI GGATG 4 cut(s) 467, 560, 972, 989
Fsp4HI GCNGC 3 cut(s) 339, 675, 999
FspBI CTAG 4 cut(s) 44, 539, 852, 905
GluI GCNGC 3 cut(s) 339, 675, 999
GsaI CCCAGC 1 cut(s) 397
GsuI CTGGAG 2 cut(s) 339, 495
HaeIII GGCC 2 cut(s) 158, 795
HapII CCGG 2 cut(s) 660, 960
Hin1II CATG 2 cut(s) 871, 948
HindIII AAGCTT 3 cut(s) 224, 646, 800
HinfI GANTC 8 cut(s) 107, 190, 210, 258, 436, 639, 872, 981
HpaII CCGG 2 cut(s) 660, 960
HphI GGTGA 3 cut(s) 445, 673, 1031
Hpy166II GTNNAC 2 cut(s) 20, 550
Hpy188I TCNGA 4 cut(s) 519, 571, 606, 777
Hpy188III TCNNGA 5 cut(s) 313, 345, 474, 717, 876
Hpy8I GTNNAC 2 cut(s) 20, 550
HpyAV CCTTC 3 cut(s) 497, 624, 1037
HpyCH4III ACNGT 3 cut(s) 61, 148, 547
HpyCH4V TGCA 2 cut(s) 497, 924
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 338, 699
HpyF3I CTNAG 5 cut(s) 140, 284, 491, 568, 694
Hsp92II CATG 2 cut(s) 871, 948
Kzo9I GATC 3 cut(s) 559, 584, 1021
LmnI GCTCC 3 cut(s) 320, 501, 623
Lsp1109I GCAGC 2 cut(s) 686, 985
LweI GCATC 2 cut(s) 489, 721
MaeI CTAG 4 cut(s) 44, 539, 852, 905
MaeIII GTNAC 3 cut(s) 640, 841, 955
MalI GATC 3 cut(s) 561, 586, 1023
MboI GATC 3 cut(s) 559, 584, 1021
MboII GAAGA 3 cut(s) 189, 219, 316
MfeI CAATTG 1 cut(s) 706
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 764
MluCI AATT 4 cut(s) 376, 563, 607, 706
MlyI GAGTC 3 cut(s) 101, 648, 881
MseI TTAA 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 2 cut(s) 660, 960
MspR9I CCNGG 5 cut(s) 254, 661, 792, 833, 961
MunI CAATTG 1 cut(s) 706
MvaI CCWGG 3 cut(s) 254, 792, 833
MwoI GCNNNNNNNGC 3 cut(s) 329, 338, 699
NciI CCSGG 2 cut(s) 661, 961
NdeII GATC 3 cut(s) 559, 584, 1021
NlaIII CATG 2 cut(s) 871, 948
NlaIV GGNNCC 2 cut(s) 31, 322
NmuCI GTSAC 3 cut(s) 640, 841, 955
NspI RCATGY 1 cut(s) 948
PaeR7I CTCGAG 1 cut(s) 928
PceI AGGCCT 1 cut(s) 795
PciI ACATGT 1 cut(s) 944
PfeI GAWTC 5 cut(s) 190, 210, 258, 436, 981
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 3 cut(s) 340, 676, 1000
PleI GAGTC 3 cut(s) 101, 647, 880
PpsI GAGTC 3 cut(s) 101, 647, 880
PscI ACATGT 1 cut(s) 944
Psp6I CCWGG 3 cut(s) 252, 790, 831
PspFI CCCAGC 1 cut(s) 393
PspGI CCWGG 3 cut(s) 252, 790, 831
PspN4I GGNNCC 2 cut(s) 31, 322
PspXI VCTCGAGB 1 cut(s) 928
PstNI CAGNNNCTG 1 cut(s) 400
PsuI RGATCY 1 cut(s) 584
SaqAI TTAA 1 cut(s) 722
SatI GCNGC 3 cut(s) 339, 675, 999
Sau3AI GATC 3 cut(s) 559, 584, 1021
SchI GAGTC 3 cut(s) 101, 648, 881
ScrFI CCNGG 5 cut(s) 254, 661, 792, 833, 961
SduI GDGCHC 1 cut(s) 764
SfaNI GCATC 2 cut(s) 489, 721
SfcI CTRYAG 2 cut(s) 111, 936
Sfr274I CTCGAG 1 cut(s) 928
SlaI CTCGAG 1 cut(s) 928
SmlI CTYRAG 4 cut(s) 715, 797, 928, 993
SmoI CTYRAG 4 cut(s) 715, 797, 928, 993
Sse9I AATT 4 cut(s) 376, 563, 607, 706
SseBI AGGCCT 1 cut(s) 795
SsiI CCGC 4 cut(s) 300, 338, 363, 653
SspMI CTAG 4 cut(s) 44, 539, 852, 905
StuI AGGCCT 1 cut(s) 795
StyD4I CCNGG 5 cut(s) 252, 659, 790, 831, 959
TaaI ACNGT 3 cut(s) 61, 148, 547
TaqI TCGA 4 cut(s) 117, 380, 875, 929
TasI AATT 4 cut(s) 376, 563, 607, 706
TauI GCSGC 1 cut(s) 341
TfiI GAWTC 5 cut(s) 190, 210, 258, 436, 981
Tru1I TTAA 1 cut(s) 722
Tru9I TTAA 1 cut(s) 722
TscAI CASTG 4 cut(s) 66, 247, 393, 511
TseFI GTSAC 3 cut(s) 640, 841, 955
TseI GCWGC 2 cut(s) 674, 998
Tsp45I GTSAC 3 cut(s) 640, 841, 955
TspDTI ATGAA 5 cut(s) 85, 502, 589, 729, 856
TspGWI ACGGA 1 cut(s) 741
TspRI CASTG 4 cut(s) 66, 247, 393, 511
XapI RAATTY 1 cut(s) 607
XceI RCATGY 1 cut(s) 948
XhoI CTCGAG 1 cut(s) 928
XmiI GTMKAC 1 cut(s) 549
XspI CTAG 4 cut(s) 44, 539, 852, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.