AT3G57270

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006449
Physical Location & Seq
Reverse (-)
21191237 .. 21193189
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G57270.1

Sequence Viewer

Length: 1023 bp
ATGGATCTCCGATTCTTGGCGTCCTTGACCCTTCTTCTCGGTCTATTCTTCGTCAATACAAATCCAACAGGTGGACAAGTCGGCGTTTGCTATGGAAGAAACGGCAACAACCTCCCTTCACCAGCAGAAACCATTGCGCTCTTCAAACAGAAAAACATCCAACGAGTTAGGCTATACAGTCCGGACCACGACGTTCTAGCCGCCCTACGTGGCTCCAACATCGAGGTAACGCTGGGTCTTCCCAACTCTTACCTACAGAGCGTGGCCTCGAGCCAATCACAAGCTAACGCGTGGGTACAAACCTACGTCATGAACTACGCGAATGGCGTCAGGTTCCGTTACATATCTGTTGGGAATGAAGTTAAAATCTCCGACTCTTACGCACAGTTCCTAGTCCCTGCCATGGAAAACATTGACCGGGCGGTTTTAGCAGCCGGACTCGGTGGTCGTATCAAGGTATCCACATCGGTAGACATGGGAGTGCTCCGAGAGTCTTATCCACCATCGAAAGGTTCGTTTAGGGGAGATGTTATGGTGGTAATGGAACCAATCATACGTTTCTTGGTAAGCAAGAACTCTCCTTTACTCTTAAACCTCTACACTTACTTCAGCTACGCGGGCAATGTAGGCCAAATCCGGCTCGATTACGCTCTCTTCACGGCTCCTTCAGGAATCGTTTCGGACCCTCCTAGATCTTACCAAAACCTATTTGATGCGATGCTCGATGCGATGTACTCGGCTCTAGAGAAATCGGGTGGCGCATCATTGGAAATAGTTGTGGCGGAGACTGGATGGCCAACGGGAGGAGGAACCGATACAAACATAGAGAATGCGAGAATTTATAACAATAATTTGATTAAGCATGTGAAGAATGGGACACCGAAGAGACCAGGGAAAGAAATCGAGACATATTTATTTGCCATTTATGACGAGAATCAAAAGCCGACTCCTCCTTATGTTGAGAAGTTTTGGGGATTGTTCTATCCCAACAAGCAGCCTAAGTATGATATTAACTTTTATTGA

Protein Analysis

340

Amino Acids

37.65

Weight (kDa)

8.69

Isoelectric Point (pI)

42.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 27 - 339 4.4e-115 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
AasI GACNNNNNNGTC 1 cut(s) 444
AccB7I CCANNNNNTGG 1 cut(s) 71
AccI GTMKAC 1 cut(s) 471
AccII CGCG 3 cut(s) 290, 320, 617
AccIII TCCGGA 1 cut(s) 181
AciI CCGC 4 cut(s) 201, 422, 617, 782
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 794
AcsI RAATTY 1 cut(s) 837
AcuI CTGAAG 2 cut(s) 592, 651
AcyI GRCGYC 2 cut(s) 20, 327
AfaI GTAC 2 cut(s) 297, 734
AfiI CCNNNNNNNGG 5 cut(s) 16, 71, 403, 509, 803
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 1 cut(s) 145
AjnI CCWGG 1 cut(s) 889
AluBI AGCT 2 cut(s) 284, 612
AluI AGCT 2 cut(s) 284, 612
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 3 cut(s) 779, 880, 899
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 268
Aor13HI TCCGGA 1 cut(s) 181
AoxI GGCC 3 cut(s) 264, 628, 794
ApeKI GCWGC 2 cut(s) 431, 994
ApoI RAATTY 1 cut(s) 837
Asp700I GAANNNNTTC 1 cut(s) 676
AspLEI GCGC 2 cut(s) 139, 761
AspS9I GGNCC 2 cut(s) 184, 682
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 1 cut(s) 111
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 2 cut(s) 184, 682
BalI TGGCCA 1 cut(s) 796
BbsI GAAGAC 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 2 cut(s) 443, 1006
BccI CCATC 2 cut(s) 511, 786
BceAI ACGGC 2 cut(s) 118, 675
BciT130I CCWGG 1 cut(s) 891
BciVI GTATCC 1 cut(s) 469
BcnI CCSGG 1 cut(s) 419
BcoDI GTCTC 3 cut(s) 779, 880, 899
BfaI CTAG 4 cut(s) 197, 392, 690, 743
BfmI CTRYAG 1 cut(s) 254
BfuI GTATCC 1 cut(s) 469
BglII AGATCT 1 cut(s) 692
BisI GCNGC 3 cut(s) 201, 432, 995
BlsI GCNGC 3 cut(s) 202, 433, 996
Bme1390I CCNGG 2 cut(s) 419, 891
Bme18I GGWCC 2 cut(s) 184, 682
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 184, 682
BmiI GGNNCC 6 cut(s) 214, 335, 546, 663, 684, 811
BmrFI CCNGG 2 cut(s) 419, 891
BmsI GCATC 4 cut(s) 703, 708, 715, 770
BpiI GAAGAC 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 419
BsaAI YACGTR 1 cut(s) 209
BsaHI GRCGYC 2 cut(s) 20, 327
BsaI GGTCTC 1 cut(s) 880
BsaJI CCNNGG 2 cut(s) 402, 890
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 5 cut(s) 16, 71, 403, 509, 803
Bse1I ACTGG 1 cut(s) 793
Bse3DI GCAATG 2 cut(s) 132, 628
BseAI TCCGGA 1 cut(s) 181
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 2 cut(s) 402, 890
BseGI GGATG 2 cut(s) 156, 797
BseLI CCNNNNNNNGG 5 cut(s) 16, 71, 403, 509, 803
BseMI GCAATG 2 cut(s) 132, 628
BseNI ACTGG 1 cut(s) 793
BseRI GAGGAG 2 cut(s) 819, 939
BseXI GCAGC 2 cut(s) 443, 1006
BseYI CCCAGC 1 cut(s) 232
Bsh1236I CGCG 3 cut(s) 290, 320, 617
BshFI GGCC 3 cut(s) 266, 630, 796
BsiHKAI GWGCWC 1 cut(s) 486
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 4 cut(s) 182, 418, 435, 637
BslFI GGGAC 2 cut(s) 380, 889
BslI CCNNNNNNNGG 5 cut(s) 16, 71, 403, 509, 803
BsmAI GTCTC 3 cut(s) 779, 880, 899
BsmFI GGGAC 2 cut(s) 380, 889
BsmI GAATGC 1 cut(s) 835
BsnI GGCC 3 cut(s) 266, 630, 796
Bso31I GGTCTC 1 cut(s) 880
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 486
Bsp13I TCCGGA 1 cut(s) 181
Bsp143I GATC 2 cut(s) 4, 692
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 4 cut(s) 201, 422, 617, 782
BspANI GGCC 3 cut(s) 266, 630, 796
BspEI TCCGGA 1 cut(s) 181
BspFNI CGCG 3 cut(s) 290, 320, 617
BspHI TCATGA 1 cut(s) 309
BspLI GGNNCC 6 cut(s) 214, 335, 546, 663, 684, 811
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 880
BsrDI GCAATG 2 cut(s) 132, 628
BsrI ACTGG 1 cut(s) 793
BssECI CCNNGG 2 cut(s) 402, 890
BssMI GATC 2 cut(s) 4, 692
BssNI GRCGYC 2 cut(s) 20, 327
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 2 cut(s) 179, 387
Bst6I CTCTTC 3 cut(s) 146, 659, 878
BstACI GRCGYC 2 cut(s) 20, 327
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 619
BstDEI CTNAG 1 cut(s) 999
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 156, 797
BstFNI CGCG 3 cut(s) 290, 320, 617
BstHHI GCGC 2 cut(s) 139, 761
BstKTI GATC 2 cut(s) 7, 695
BstMAI GTCTC 3 cut(s) 779, 880, 899
BstMBI GATC 2 cut(s) 4, 692
BstMWI GCNNNNNNNGC 3 cut(s) 428, 618, 627
BstNI CCWGG 1 cut(s) 891
BstNSI RCATGY 1 cut(s) 866
BstSCI CCNGG 2 cut(s) 417, 889
BstSFI CTRYAG 1 cut(s) 254
BstUI CGCG 3 cut(s) 290, 320, 617
BstV1I GCAGC 2 cut(s) 443, 1006
BstV2I GAAGAC 1 cut(s) 230
BstX2I RGATCY 2 cut(s) 4, 692
BstYI RGATCY 2 cut(s) 4, 692
BsuI GTATCC 1 cut(s) 469
BsuRI GGCC 3 cut(s) 266, 630, 796
BtgI CCRYGG 1 cut(s) 402
BtgZI GCGATG 2 cut(s) 731, 743
BtsCI GGATG 2 cut(s) 156, 797
Cac8I GCNNGC 1 cut(s) 619
CciI TCATGA 1 cut(s) 309
CfoI GCGC 2 cut(s) 139, 761
Cfr13I GGNCC 2 cut(s) 184, 682
CseI GACGC 2 cut(s) 9, 316
Csp6I GTAC 2 cut(s) 296, 733
CviAII CATG 4 cut(s) 310, 403, 475, 863
CviQI GTAC 2 cut(s) 296, 733
DdeI CTNAG 1 cut(s) 999
DpnI GATC 2 cut(s) 6, 694
DpnII GATC 2 cut(s) 4, 692
DrdI GACNNNNNNGTC 1 cut(s) 444
DseDI GACNNNNNNGTC 1 cut(s) 444
EaeI YGGCCR 1 cut(s) 794
Eam1104I CTCTTC 3 cut(s) 146, 659, 878
EarI CTCTTC 3 cut(s) 146, 659, 878
EciI GGCGGA 1 cut(s) 797
Eco130I CCWWGG 1 cut(s) 402
Eco31I GGTCTC 1 cut(s) 880
Eco47I GGWCC 2 cut(s) 184, 682
Eco57I CTGAAG 2 cut(s) 592, 651
Eco88I CYCGRG 1 cut(s) 268
EcoRII CCWGG 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 4 cut(s) 313, 406, 478, 866
FaqI GGGAC 2 cut(s) 380, 889
FatI CATG 4 cut(s) 309, 402, 474, 862
FauI CCCGC 1 cut(s) 610
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 3 cut(s) 201, 432, 995
FokI GGATG 2 cut(s) 143, 804
Fsp4HI GCNGC 3 cut(s) 201, 432, 995
FspBI CTAG 4 cut(s) 197, 392, 690, 743
GlaI GCGC 2 cut(s) 138, 760
GluI GCNGC 3 cut(s) 201, 432, 995
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 3 cut(s) 266, 630, 796
HapII CCGG 4 cut(s) 182, 418, 435, 637
HgaI GACGC 2 cut(s) 9, 316
HhaI GCGC 2 cut(s) 139, 761
Hin1I GRCGYC 2 cut(s) 20, 327
Hin1II CATG 4 cut(s) 313, 406, 478, 866
Hin6I GCGC 2 cut(s) 137, 759
HinP1I GCGC 2 cut(s) 137, 759
HinfI GANTC 7 cut(s) 12, 374, 438, 491, 672, 934, 946
HpaII CCGG 4 cut(s) 182, 418, 435, 637
HphI GGTGA 1 cut(s) 111
Hpy166II GTNNAC 2 cut(s) 74, 472
Hpy188I TCNGA 4 cut(s) 11, 373, 488, 682
Hpy188III TCNNGA 5 cut(s) 182, 310, 669, 743, 904
Hpy8I GTNNAC 2 cut(s) 74, 472
Hpy99I CGWCG 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 41, 126, 675
HpyCH4III ACNGT 2 cut(s) 179, 387
HpyCH4IV ACGT 4 cut(s) 192, 208, 306, 556
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 618, 627
HpyF3I CTNAG 1 cut(s) 999
HpySE526I ACGT 4 cut(s) 192, 208, 306, 556
Hsp92I GRCGYC 2 cut(s) 20, 327
Hsp92II CATG 4 cut(s) 313, 406, 478, 866
HspAI GCGC 2 cut(s) 137, 759
Kpn2I TCCGGA 1 cut(s) 181
Kzo9I GATC 2 cut(s) 4, 692
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 3 cut(s) 218, 489, 667
Lsp1109I GCAGC 2 cut(s) 443, 1006
LweI GCATC 4 cut(s) 703, 708, 715, 770
MaeI CTAG 4 cut(s) 197, 392, 690, 743
MaeII ACGT 4 cut(s) 192, 208, 306, 556
MaeIII GTNAC 2 cut(s) 226, 338
MalI GATC 2 cut(s) 6, 694
MboI GATC 2 cut(s) 4, 692
MboII GAAGA 8 cut(s) 26, 40, 108, 133, 230, 646, 880, 895
MflI RGATCY 2 cut(s) 4, 692
MhlI GDGCHC 1 cut(s) 486
MlsI TGGCCA 1 cut(s) 796
MluCI AATT 2 cut(s) 837, 850
MluI ACGCGT 1 cut(s) 288
MluNI TGGCCA 1 cut(s) 796
MlyI GAGTC 4 cut(s) 368, 432, 500, 940
MmeI TCCRAC 4 cut(s) 89, 184, 240, 396
MnlI CCTC 8 cut(s) 122, 217, 277, 605, 696, 797, 800, 960
Mox20I TGGCCA 1 cut(s) 796
MroI TCCGGA 1 cut(s) 181
MroXI GAANNNNTTC 1 cut(s) 676
MscI TGGCCA 1 cut(s) 796
MseI TTAA 4 cut(s) 363, 590, 858, 1011
MslI CAYNNNNRTG 1 cut(s) 479
Msp20I TGGCCA 1 cut(s) 796
MspI CCGG 4 cut(s) 182, 418, 435, 637
MspR9I CCNGG 2 cut(s) 419, 891
Mva1269I GAATGC 1 cut(s) 835
MvaI CCWGG 1 cut(s) 891
MvnI CGCG 3 cut(s) 290, 320, 617
MwoI GCNNNNNNNGC 3 cut(s) 428, 618, 627
NciI CCSGG 1 cut(s) 419
NcoI CCATGG 1 cut(s) 402
NdeII GATC 2 cut(s) 4, 692
NlaIII CATG 4 cut(s) 313, 406, 478, 866
NlaIV GGNNCC 6 cut(s) 214, 335, 546, 663, 684, 811
NmeAIII GCCGAG 1 cut(s) 716
NspI RCATGY 1 cut(s) 866
PaeR7I CTCGAG 1 cut(s) 268
PagI TCATGA 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 2 cut(s) 324, 512
PctI GAATGC 1 cut(s) 835
PdmI GAANNNNTTC 1 cut(s) 676
PfeI GAWTC 3 cut(s) 12, 672, 934
PflMI CCANNNNNTGG 1 cut(s) 71
PkrI GCNGC 3 cut(s) 202, 433, 996
PleI GAGTC 4 cut(s) 368, 432, 499, 940
PpsI GAGTC 4 cut(s) 368, 432, 499, 940
Ppu21I YACGTR 1 cut(s) 209
PsiI TTATAA 1 cut(s) 843
Psp6I CCWGG 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 6 cut(s) 214, 335, 546, 663, 684, 811
PspPI GGNCC 2 cut(s) 184, 682
PspXI VCTCGAGB 1 cut(s) 268
PsrI GAACNNNNNNTAC 2 cut(s) 537, 569
PsuI RGATCY 2 cut(s) 4, 692
RsaI GTAC 2 cut(s) 297, 734
RsaNI GTAC 2 cut(s) 296, 733
RseI CAYNNNNRTG 1 cut(s) 479
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 4 cut(s) 363, 590, 858, 1011
SatI GCNGC 3 cut(s) 201, 432, 995
Sau3AI GATC 2 cut(s) 4, 692
Sau96I GGNCC 2 cut(s) 184, 682
SchI GAGTC 4 cut(s) 368, 432, 500, 940
ScrFI CCNGG 2 cut(s) 419, 891
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 4 cut(s) 703, 708, 715, 770
SfcI CTRYAG 1 cut(s) 254
Sfr274I CTCGAG 1 cut(s) 268
SinI GGWCC 2 cut(s) 184, 682
SlaI CTCGAG 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 2 cut(s) 837, 850
SsiI CCGC 4 cut(s) 201, 422, 617, 782
SspMI CTAG 4 cut(s) 197, 392, 690, 743
StyD4I CCNGG 2 cut(s) 417, 889
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 179, 387
TaiI ACGT 4 cut(s) 195, 211, 309, 559
TaqI TCGA 6 cut(s) 222, 269, 506, 642, 723, 903
TaqII GACCGA 1 cut(s) 29
TasI AATT 2 cut(s) 837, 850
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 3 cut(s) 12, 672, 934
Tru1I TTAA 4 cut(s) 363, 590, 858, 1011
Tru9I TTAA 4 cut(s) 363, 590, 858, 1011
TseI GCWGC 2 cut(s) 431, 994
TspDTI ATGAA 2 cut(s) 326, 372
TspGWI ACGGA 1 cut(s) 326
Van91I CCANNNNNTGG 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 184, 682
XapI RAATTY 1 cut(s) 837
XbaI TCTAGA 1 cut(s) 742
XceI RCATGY 1 cut(s) 866
XhoI CTCGAG 1 cut(s) 268
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 676
XspI CTAG 4 cut(s) 197, 392, 690, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.