MD14G1078700.v1.1

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0006449
Physical Location & Seq
Forward (+)
9003314 .. 9004075
762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1078700.v1.1.491

Sequence Viewer

Length: 762 bp
ATGATTGGCGATGACCTACCAACCCAAGCAGAAGTTATTGATCTCTACAAAACATATAAAATCCAGAGAATGAGACTCTATGATCCAAACCAAGCTGCTTTGGGAGCCCTTAGAAACTCTAATATTGAGCTCTTGCTAGGCGTGCCAAATGACAACCTTCAAAACCTTGCTTCAAGCCAAGCCAATGCAAACACATGGGTGCAAAACAATGTGAGAAACTATGCCAATGTAAGATTCAAATACATTGCGGTAGGAAATGAAGTCAAGCCCTCAAATTCGTTTGCCCGATTTCTGGTCCTAGCAATGCGGAATATAGAGAAGGCGATTTCTCTTGCTGACCTTGCCAAACAAATAAAAGTTTCCACTGCCATAGGCACTGGGGTACTTGGAGAAGCCTATCCTCCGTCAAATTGCTCATTCAAGTCTGAATATAGCTTACTTTTGCAACCCATTATCCGTTTCCTCGTGAATCACATTTCACCATTACTTGTTAACTTGTACCCGTATTTTAGTTACAGCAGCAACACTCGTGATATTCGTCTTGATTATGCTATTTTCACATCTCCATCAATTGTGGTACAAGATGGAAAATTTGGTTATGATAATCTTTTCGATGCCATTTTGGATGGTGTTTATGCTGCCCTTGAGAAGGTTGGAGGAGGGTCCTTGGAAATTGTTGTATCAGAGACGGGCTGGCCATCAGCTGGTGGAACGGAAACGATAATTGATAATGCAAAGACATATTACTCACACTTGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

254

Amino Acids

27.94

Weight (kDa)

5.73

Isoelectric Point (pI)

38.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 1 - 253 8.7e-81 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 704
AciI CCGC 2 cut(s) 248, 307
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 1 cut(s) 695
AcsI RAATTY 2 cut(s) 274, 590
AfaI GTAC 3 cut(s) 384, 500, 579
AfiI CCNNNNNNNGG 3 cut(s) 292, 649, 704
AgsI TTSAA 4 cut(s) 161, 174, 238, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AleI CACNNNNGTG 1 cut(s) 197
AluBI AGCT 4 cut(s) 95, 130, 435, 704
AluI AGCT 4 cut(s) 95, 130, 435, 704
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 67, 680
AlwI GGATC 1 cut(s) 77
AoxI GGCC 1 cut(s) 695
ApeKI GCWGC 3 cut(s) 95, 519, 638
ApoI RAATTY 2 cut(s) 274, 590
AspS9I GGNCC 2 cut(s) 295, 663
AsuHPI GGTGA 1 cut(s) 471
AvaII GGWCC 2 cut(s) 295, 663
BalI TGGCCA 1 cut(s) 697
BanII GRGCYC 2 cut(s) 109, 132
BauI CACGAG 2 cut(s) 464, 528
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 82, 531, 625
BccI CCATC 4 cut(s) 574, 578, 620, 706
BcoDI GTCTC 2 cut(s) 67, 680
BfaI CTAG 2 cut(s) 137, 299
BisI GCNGC 3 cut(s) 96, 520, 639
BlsI GCNGC 3 cut(s) 97, 521, 640
Bme18I GGWCC 2 cut(s) 295, 663
BmgT120I GGNCC 2 cut(s) 295, 663
BmiI GGNNCC 2 cut(s) 106, 664
BmrI ACTGGG 1 cut(s) 387
BmsI GCATC 1 cut(s) 604
BmuI ACTGGG 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 665
BsaJI CCNNGG 1 cut(s) 666
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 649, 704
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 2 cut(s) 243, 309
BseDI CCNNGG 1 cut(s) 666
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 3 cut(s) 292, 649, 704
BseMI GCAATG 2 cut(s) 243, 309
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 1 cut(s) 672
BseXI GCAGC 3 cut(s) 82, 531, 625
BshFI GGCC 1 cut(s) 697
BsiHKAI GWGCWC 1 cut(s) 132
BslI CCNNNNNNNGG 3 cut(s) 292, 649, 704
BsmAI GTCTC 2 cut(s) 67, 680
BsmBI CGTCTC 1 cut(s) 680
BsnI GGCC 1 cut(s) 697
Bsp1286I GDGCHC 2 cut(s) 109, 132
Bsp143I GATC 2 cut(s) 40, 82
BspACI CCGC 2 cut(s) 248, 307
BspANI GGCC 1 cut(s) 697
BspLI GGNNCC 2 cut(s) 106, 664
BspPI GGATC 1 cut(s) 77
BsrDI GCAATG 2 cut(s) 243, 309
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 666
BssMI GATC 2 cut(s) 40, 82
BssSI CACGAG 2 cut(s) 464, 528
BssT1I CCWWGG 1 cut(s) 666
Bst2BI CACGAG 2 cut(s) 464, 528
BstC8I GCNNGC 2 cut(s) 143, 695
BstDEI CTNAG 1 cut(s) 110
BstENI CCTNNNNNAGG 1 cut(s) 647
BstF5I GGATG 1 cut(s) 631
BstKTI GATC 2 cut(s) 43, 85
BstMAI GTCTC 2 cut(s) 67, 680
BstMBI GATC 2 cut(s) 40, 82
BstMWI GCNNNNNNNGC 3 cut(s) 104, 142, 341
BstV1I GCAGC 3 cut(s) 82, 531, 625
BsuRI GGCC 1 cut(s) 697
BtgZI GCGATG 1 cut(s) 24
BtsCI GGATG 1 cut(s) 631
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 2 cut(s) 363, 375
Cac8I GCNNGC 2 cut(s) 143, 695
Cfr13I GGNCC 2 cut(s) 295, 663
Csp6I GTAC 3 cut(s) 383, 499, 578
CviAII CATG 1 cut(s) 195
CviQI GTAC 3 cut(s) 383, 499, 578
DdeI CTNAG 1 cut(s) 110
DpnI GATC 2 cut(s) 42, 84
DpnII GATC 2 cut(s) 40, 82
EaeI YGGCCR 1 cut(s) 695
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 666
Eco24I GRGCYC 2 cut(s) 109, 132
Eco47I GGWCC 2 cut(s) 295, 663
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 663
EcoT14I CCWWGG 1 cut(s) 666
EcoT38I GRGCYC 2 cut(s) 109, 132
ErhI CCWWGG 1 cut(s) 666
Esp3I CGTCTC 1 cut(s) 680
FaeI CATG 1 cut(s) 198
FatI CATG 1 cut(s) 194
Fnu4HI GCNGC 3 cut(s) 96, 520, 639
FokI GGATG 1 cut(s) 638
FriOI GRGCYC 2 cut(s) 109, 132
Fsp4HI GCNGC 3 cut(s) 96, 520, 639
FspBI CTAG 2 cut(s) 137, 299
GluI GCNGC 3 cut(s) 96, 520, 639
HaeIII GGCC 1 cut(s) 697
Hin1II CATG 1 cut(s) 198
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 3 cut(s) 75, 234, 469
HpaI GTTAAC 1 cut(s) 493
HphI GGTGA 1 cut(s) 471
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 2 cut(s) 427, 685
Hpy188III TCNNGA 4 cut(s) 64, 466, 530, 542
Hpy8I GTNNAC 1 cut(s) 493
HpyAV CCTTC 3 cut(s) 167, 313, 643
HpyCH4V TGCA 4 cut(s) 188, 202, 445, 734
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 142, 341
HpyF3I CTNAG 1 cut(s) 110
Hsp92II CATG 1 cut(s) 198
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 2 cut(s) 40, 82
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 5 cut(s) 77, 278, 363, 679, 690
Lsp1109I GCAGC 3 cut(s) 82, 531, 625
LweI GCATC 1 cut(s) 604
MaeI CTAG 2 cut(s) 137, 299
MaeIII GTNAC 1 cut(s) 512
MalI GATC 2 cut(s) 42, 84
MboI GATC 2 cut(s) 40, 82
MfeI CAATTG 1 cut(s) 570
MhlI GDGCHC 2 cut(s) 109, 132
MlsI TGGCCA 1 cut(s) 697
MluCI AATT 6 cut(s) 274, 409, 570, 590, 672, 723
MluNI TGGCCA 1 cut(s) 697
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 1 cut(s) 634
MnlI CCTC 5 cut(s) 280, 411, 473, 650, 653
Mox20I TGGCCA 1 cut(s) 697
MscI TGGCCA 1 cut(s) 697
MseI TTAA 2 cut(s) 492, 760
MslI CAYNNNNRTG 1 cut(s) 197
Msp20I TGGCCA 1 cut(s) 697
MspA1I CMGCKG 1 cut(s) 704
MunI CAATTG 1 cut(s) 570
MwoI GCNNNNNNNGC 3 cut(s) 104, 142, 341
NdeII GATC 2 cut(s) 40, 82
NlaIII CATG 1 cut(s) 198
NlaIV GGNNCC 2 cut(s) 106, 664
OliI CACNNNNGTG 1 cut(s) 197
PcsI WCGNNNNNNNCGW 1 cut(s) 535
PfeI GAWTC 2 cut(s) 234, 469
PflMI CCANNNNNTGG 1 cut(s) 704
PkrI GCNGC 3 cut(s) 97, 521, 640
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
PpuMI RGGWCCY 1 cut(s) 663
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 663
PspN4I GGNNCC 2 cut(s) 106, 664
PspPI GGNCC 2 cut(s) 295, 663
PspPPI RGGWCCY 1 cut(s) 663
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 3 cut(s) 384, 500, 579
RsaNI GTAC 3 cut(s) 383, 499, 578
RseI CAYNNNNRTG 1 cut(s) 197
SacI GAGCTC 1 cut(s) 132
SaqAI TTAA 2 cut(s) 492, 760
SatI GCNGC 3 cut(s) 96, 520, 639
Sau3AI GATC 2 cut(s) 40, 82
Sau96I GGNCC 2 cut(s) 295, 663
SchI GAGTC 1 cut(s) 69
SduI GDGCHC 2 cut(s) 109, 132
SetI ASST 9 cut(s) 18, 97, 132, 159, 168, 342, 437, 654, 706
SfaNI GCATC 1 cut(s) 604
SinI GGWCC 2 cut(s) 295, 663
SmiMI CAYNNNNRTG 1 cut(s) 197
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 6 cut(s) 274, 409, 570, 590, 672, 723
SsiI CCGC 2 cut(s) 248, 307
SspI AATATT 1 cut(s) 124
SspMI CTAG 2 cut(s) 137, 299
SstI GAGCTC 1 cut(s) 132
StyI CCWWGG 1 cut(s) 666
TaqI TCGA 1 cut(s) 612
TasI AATT 6 cut(s) 274, 409, 570, 590, 672, 723
TfiI GAWTC 2 cut(s) 234, 469
Tru1I TTAA 2 cut(s) 492, 760
Tru9I TTAA 2 cut(s) 492, 760
TscAI CASTG 2 cut(s) 370, 382
TseI GCWGC 3 cut(s) 95, 519, 638
TspDTI ATGAA 1 cut(s) 273
TspGWI ACGGA 3 cut(s) 393, 446, 728
TspRI CASTG 2 cut(s) 370, 382
Van91I CCANNNNNTGG 1 cut(s) 704
VpaK11BI GGWCC 2 cut(s) 295, 663
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 2 cut(s) 274, 590
XspI CTAG 2 cut(s) 137, 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.