Rroxscaffold_3G00275110

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006449
Physical Location & Seq
Forward (+)
66665589 .. 66667231
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00275110.1

Sequence Viewer

Length: 1044 bp
ATGATCATGTCTAAATGCAATTCTTCAGTTGGCAAAACCCCTTCTCTGATCTCCATCGTGTTTCTACTTGGGCAGCTCATGGTGATGACCCTACAAACAGCAGATGCCCAAATTGGTGTATGCTATGGAATGTTAGGCAACTTACCACCTCAAACAGATGTTGTTGCTCTTTACAAACAATACAACATTCGAAGGATGCGACTCTATGATCCAAACCAAGCCGCTCTCACTGCCCTGAGGGGCTCTAACATCGAGCTCATTCTCGGCGTCCCAAACGACAACCTTCGAACCATTGCCTCAAGCCAGGCCAATGCAAACTCATGGGTTCAAAACAATGTAAGAAACTACGGCGATGTAAGATTCAAGTACATTGCTGTCGGAAACGAAGTCCAGCCAGAAGAATCATCTGCACAGTTTCTAGTCCCGGCCATGCGAAACATTCAAACCGCAATTGCCAATGCTGGTCTTGGCAACCAAATCAAAGTTTCCACAGCCATCGACACCAGAGGACTCGGAGAATCGTACCCTCCCTCAAAAGGTTCCTTCAAGTCTGAAAATAGAGCGCTGCTCGATCCCATTATACGCTTCTTAGTGGAGCATAGATCTCCATTGCTGGTTAACCTGTACCCGTATTTTAGTTACAAGGACAACACCGGTGCCATTCGTCTCGATTATGCTCTCTTCACAGCTGCATCGGTTGTTGTACAAGATGGCCAACTTGGTTATCGTAATCTTTTCGATGCCATGTTGGACGCTGTGTATTCTGCTCTTGAGAAGGCCGGTGGAGGGTCTTTGGAAATTGTTGTATCGGAGAGCGGTTGGCCAACGGCCGGTGGAACAGCAACTACTGTTGACAATGCAAGGACTTACAACTCAAACTTGATTCAACATGTCAAGGGTGGGACTCCGAAAAAGCCCGGGAGGCCGATAGAAACTTACATATTTGCCATGTTTGATGAGGATAGGAAAAATCCAGAGCTTGAGAAACATTGGGGGCTGTTTTCCCCGAACAAACAACCAAAATACCCTATCAATTTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

347

Amino Acids

38.13

Weight (kDa)

8.86

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 38 - 346 3.1e-118 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 656
AccBSI CCGCTC 2 cut(s) 224, 816
AciI CCGC 3 cut(s) 222, 447, 816
AclWI GGATC 2 cut(s) 203, 566
AcoI YGGCCR 4 cut(s) 426, 712, 821, 828
AcuI CTGAAG 1 cut(s) 9
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 4 cut(s) 368, 524, 626, 705
AfeI AGCGCT 1 cut(s) 564
AfiI CCNNNNNNNGG 3 cut(s) 536, 786, 830
AflIII ACRYGT 1 cut(s) 889
AgeI ACCGGT 1 cut(s) 653
AgsI TTSAA 5 cut(s) 329, 364, 443, 547, 887
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 4 cut(s) 76, 256, 689, 979
AluI AGCT 4 cut(s) 76, 256, 689, 979
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 671
AlwI GGATC 2 cut(s) 203, 566
Ama87I CYCGRG 1 cut(s) 917
Aor51HI AGCGCT 1 cut(s) 564
AoxI GGCC 7 cut(s) 306, 426, 712, 777, 821, 828, 923
ApeKI GCWGC 3 cut(s) 73, 565, 689
AsiGI ACCGGT 1 cut(s) 653
AspLEI GCGC 1 cut(s) 565
AsuC2I CCSGG 3 cut(s) 425, 918, 919
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 2 cut(s) 190, 286
AvaI CYCGRG 1 cut(s) 917
AxyI CCTNAGG 1 cut(s) 236
BalI TGGCCA 2 cut(s) 714, 823
BanI GGYRCC 1 cut(s) 656
BanII GRGCYC 2 cut(s) 245, 258
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 3 cut(s) 85, 552, 676
BccI CCATC 3 cut(s) 62, 503, 704
BceAI ACGGC 2 cut(s) 364, 843
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 3 cut(s) 425, 918, 919
BcoDI GTCTC 1 cut(s) 671
BfaI CTAG 1 cut(s) 419
BfoI RGCGCY 1 cut(s) 566
BglI GCCNNNNNGGC 1 cut(s) 922
BglII AGATCT 1 cut(s) 602
BisI GCNGC 4 cut(s) 74, 222, 566, 690
BlsI GCNGC 4 cut(s) 75, 223, 567, 691
Bme1390I CCNGG 4 cut(s) 305, 425, 918, 919
BmeT110I CYCGRG 1 cut(s) 917
BmiI GGNNCC 2 cut(s) 541, 658
BmrFI CCNGG 4 cut(s) 305, 425, 918, 919
BmsI GCATC 4 cut(s) 94, 186, 701, 730
BplI GAGNNNNNCTC 2 cut(s) 552, 584
Bpu14I TTCGAA 2 cut(s) 190, 286
BpuEI CTTGAG 3 cut(s) 283, 791, 1001
BpuMI CCSGG 3 cut(s) 425, 918, 919
BsaBI GATNNNNATC 1 cut(s) 53
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 1 cut(s) 917
BsaWI WCCGGW 1 cut(s) 653
Bsc4I CCNNNNNNNGG 3 cut(s) 536, 786, 830
Bse118I RCCGGY 3 cut(s) 653, 779, 830
Bse21I CCTNAGG 1 cut(s) 236
Bse3DI GCAATG 3 cut(s) 291, 369, 608
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 1 cut(s) 917
BseGI GGATG 1 cut(s) 201
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 3 cut(s) 536, 786, 830
BseMI GCAATG 3 cut(s) 291, 369, 608
BseMII CTCAG 1 cut(s) 227
BseX3I CGGCCG 1 cut(s) 828
BseXI GCAGC 3 cut(s) 85, 552, 676
BsgI GTGCAG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 831
BshFI GGCC 7 cut(s) 308, 428, 714, 779, 823, 830, 925
BshNI GGYRCC 1 cut(s) 656
BshTI ACCGGT 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 831
BsiHKAI GWGCWC 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 917
BsiSI CCGG 5 cut(s) 425, 654, 780, 831, 918
BslFI GGGAC 3 cut(s) 254, 407, 916
BslI CCNNNNNNNGG 3 cut(s) 536, 786, 830
BsmAI GTCTC 1 cut(s) 671
BsmBI CGTCTC 1 cut(s) 671
BsmFI GGGAC 3 cut(s) 254, 407, 916
BsnI GGCC 7 cut(s) 308, 428, 714, 779, 823, 830, 925
BsoBI CYCGRG 1 cut(s) 917
Bsp119I TTCGAA 2 cut(s) 190, 286
Bsp1286I GDGCHC 2 cut(s) 245, 258
Bsp1407I TGTACA 1 cut(s) 703
Bsp143I GATC 5 cut(s) 3, 48, 208, 571, 602
BspACI CCGC 3 cut(s) 222, 447, 816
BspANI GGCC 7 cut(s) 308, 428, 714, 779, 823, 830, 925
BspCNI CTCAG 1 cut(s) 228
BspLI GGNNCC 2 cut(s) 541, 658
BspPI GGATC 2 cut(s) 203, 566
BspT104I TTCGAA 2 cut(s) 190, 286
BspT107I GGYRCC 1 cut(s) 656
BsrBI CCGCTC 2 cut(s) 224, 816
BsrDI GCAATG 3 cut(s) 291, 369, 608
BsrFI RCCGGY 3 cut(s) 653, 779, 830
BsrGI TGTACA 1 cut(s) 703
BssAI RCCGGY 3 cut(s) 653, 779, 830
BssECI CCNNGG 1 cut(s) 917
BssMI GATC 5 cut(s) 3, 48, 208, 571, 602
BssNI GRCGYC 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 414, 850
Bst6I CTCTTC 1 cut(s) 686
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 703
BstBI TTCGAA 2 cut(s) 190, 286
BstDEI CTNAG 2 cut(s) 236, 589
BstF5I GGATG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 566
BstHHI GCGC 1 cut(s) 565
BstKTI GATC 5 cut(s) 6, 51, 211, 574, 605
BstMAI GTCTC 1 cut(s) 671
BstMBI GATC 5 cut(s) 3, 48, 208, 571, 602
BstMCI CGRYCG 1 cut(s) 831
BstMWI GCNNNNNNNGC 2 cut(s) 230, 922
BstNI CCWGG 1 cut(s) 305
BstNSI RCATGY 1 cut(s) 893
BstSCI CCNGG 4 cut(s) 303, 423, 916, 917
BstV1I GCAGC 3 cut(s) 85, 552, 676
BstX2I RGATCY 1 cut(s) 602
BstYI RGATCY 1 cut(s) 602
BstZI CGGCCG 1 cut(s) 828
Bsu36I CCTNAGG 1 cut(s) 236
BsuRI GGCC 7 cut(s) 308, 428, 714, 779, 823, 830, 925
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 1 cut(s) 201
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 228
CfoI GCGC 1 cut(s) 565
Cfr10I RCCGGY 3 cut(s) 653, 779, 830
Cfr9I CCCGGG 1 cut(s) 917
CseI GACGC 2 cut(s) 256, 761
Csp6I GTAC 4 cut(s) 367, 523, 625, 704
CspAI ACCGGT 1 cut(s) 653
CviAII CATG 7 cut(s) 7, 79, 321, 430, 745, 890, 949
CviQI GTAC 4 cut(s) 367, 523, 625, 704
DdeI CTNAG 2 cut(s) 236, 589
DpnI GATC 5 cut(s) 5, 50, 210, 573, 604
DpnII GATC 5 cut(s) 3, 48, 208, 571, 602
EaeI YGGCCR 4 cut(s) 426, 712, 821, 828
EagI CGGCCG 1 cut(s) 828
Eam1104I CTCTTC 1 cut(s) 686
EarI CTCTTC 1 cut(s) 686
Ecl136II GAGCTC 1 cut(s) 256
EclXI CGGCCG 1 cut(s) 828
Eco24I GRGCYC 2 cut(s) 245, 258
Eco47III AGCGCT 1 cut(s) 564
Eco52I CGGCCG 1 cut(s) 828
Eco53kI GAGCTC 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 9
Eco81I CCTNAGG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 917
EcoICRI GAGCTC 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 303
EcoT38I GRGCYC 2 cut(s) 245, 258
Esp3I CGTCTC 1 cut(s) 671
FaeI CATG 7 cut(s) 10, 82, 324, 433, 748, 893, 952
FaqI GGGAC 3 cut(s) 254, 407, 916
FatI CATG 7 cut(s) 6, 78, 320, 429, 744, 889, 948
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 4 cut(s) 74, 222, 566, 690
FokI GGATG 1 cut(s) 208
FriOI GRGCYC 2 cut(s) 245, 258
Fsp4HI GCNGC 4 cut(s) 74, 222, 566, 690
FspBI CTAG 1 cut(s) 419
GlaI GCGC 1 cut(s) 564
GluI GCNGC 4 cut(s) 74, 222, 566, 690
HaeII RGCGCY 1 cut(s) 566
HaeIII GGCC 7 cut(s) 308, 428, 714, 779, 823, 830, 925
HapII CCGG 5 cut(s) 425, 654, 780, 831, 918
HgaI GACGC 2 cut(s) 256, 761
HhaI GCGC 1 cut(s) 565
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 7 cut(s) 10, 82, 324, 433, 748, 893, 952
Hin6I GCGC 1 cut(s) 563
HinP1I GCGC 1 cut(s) 563
HincII GTYRAC 2 cut(s) 619, 853
HindII GTYRAC 2 cut(s) 619, 853
HinfI GANTC 7 cut(s) 201, 360, 401, 510, 518, 883, 904
HpaI GTTAAC 1 cut(s) 619
HpaII CCGG 5 cut(s) 425, 654, 780, 831, 918
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 619, 853
Hpy188I TCNGA 6 cut(s) 48, 380, 515, 553, 811, 909
Hpy188III TCNNGA 3 cut(s) 668, 770, 974
Hpy8I GTNNAC 2 cut(s) 619, 853
HpyAV CCTTC 5 cut(s) 51, 186, 293, 553, 769
HpyCH4III ACNGT 2 cut(s) 414, 850
HpyCH4V TGCA 5 cut(s) 18, 314, 410, 692, 860
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 922
HpyF3I CTNAG 2 cut(s) 236, 589
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 7 cut(s) 10, 82, 324, 433, 748, 893, 952
HspAI GCGC 1 cut(s) 563
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 619
Kzo9I GATC 5 cut(s) 3, 48, 208, 571, 602
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 3 cut(s) 85, 552, 676
LweI GCATC 4 cut(s) 94, 186, 701, 730
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 638
MalI GATC 5 cut(s) 5, 50, 210, 573, 604
MbiI CCGCTC 2 cut(s) 224, 816
MboI GATC 5 cut(s) 3, 48, 208, 571, 602
MboII GAAGA 3 cut(s) 15, 410, 673
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 602
MhlI GDGCHC 2 cut(s) 245, 258
MlsI TGGCCA 2 cut(s) 714, 823
MluCI AATT 5 cut(s) 19, 111, 450, 798, 1033
MluNI TGGCCA 2 cut(s) 714, 823
MlyI GAGTC 3 cut(s) 195, 504, 898
MmeI TCCRAC 2 cut(s) 358, 729
MnlI CCTC 9 cut(s) 159, 231, 307, 500, 537, 541, 779, 915, 952
Mox20I TGGCCA 2 cut(s) 714, 823
MscI TGGCCA 2 cut(s) 714, 823
MseI TTAA 1 cut(s) 618
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 2 cut(s) 714, 823
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 5 cut(s) 425, 654, 780, 831, 918
MspR9I CCNGG 4 cut(s) 305, 425, 918, 919
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 305
MwoI GCNNNNNNNGC 2 cut(s) 230, 922
NciI CCSGG 3 cut(s) 425, 918, 919
NdeII GATC 5 cut(s) 3, 48, 208, 571, 602
NlaIII CATG 7 cut(s) 10, 82, 324, 433, 748, 893, 952
NlaIV GGNNCC 2 cut(s) 541, 658
NmeAIII GCCGAG 1 cut(s) 243
NspI RCATGY 1 cut(s) 893
NspV TTCGAA 2 cut(s) 190, 286
PciI ACATGT 1 cut(s) 889
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PfeI GAWTC 4 cut(s) 360, 401, 518, 883
PinAI ACCGGT 1 cut(s) 653
PkrI GCNGC 4 cut(s) 75, 223, 567, 691
PleI GAGTC 3 cut(s) 195, 504, 898
PpsI GAGTC 3 cut(s) 195, 504, 898
PscI ACATGT 1 cut(s) 889
Psp124BI GAGCTC 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 541, 658
PsrI GAACNNNNNNTAC 2 cut(s) 829, 861
PsuI RGATCY 1 cut(s) 602
PvuII CAGCTG 1 cut(s) 689
RsaI GTAC 4 cut(s) 368, 524, 626, 705
RsaNI GTAC 4 cut(s) 367, 523, 625, 704
RseI CAYNNNNRTG 1 cut(s) 83
SacI GAGCTC 1 cut(s) 258
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 4 cut(s) 74, 222, 566, 690
Sau3AI GATC 5 cut(s) 3, 48, 208, 571, 602
SchI GAGTC 3 cut(s) 195, 504, 898
ScrFI CCNGG 4 cut(s) 305, 425, 918, 919
SduI GDGCHC 2 cut(s) 245, 258
SetI ASST 8 cut(s) 78, 151, 258, 285, 541, 624, 691, 981
SfaNI GCATC 4 cut(s) 94, 186, 701, 730
SfuI TTCGAA 2 cut(s) 190, 286
SgrAI CRCCGGYG 1 cut(s) 653
SmaI CCCGGG 1 cut(s) 919
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 3 cut(s) 298, 770, 980
SmoI CTYRAG 3 cut(s) 298, 770, 980
Sse9I AATT 5 cut(s) 19, 111, 450, 798, 1033
SsiI CCGC 3 cut(s) 222, 447, 816
SspMI CTAG 1 cut(s) 419
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 4 cut(s) 303, 423, 916, 917
TaaI ACNGT 2 cut(s) 414, 850
TaqI TCGA 7 cut(s) 190, 252, 286, 498, 570, 669, 738
TasI AATT 5 cut(s) 19, 111, 450, 798, 1033
TatI WGTACW 2 cut(s) 366, 703
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 4 cut(s) 360, 401, 518, 883
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 1 cut(s) 235
TseI GCWGC 3 cut(s) 73, 565, 689
TspMI CCCGGG 1 cut(s) 917
TspRI CASTG 1 cut(s) 235
XceI RCATGY 1 cut(s) 893
XmaI CCCGGG 1 cut(s) 917
XspI CTAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.