Rh7AG013500

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0006449
Physical Location & Seq
Reverse (-)
886519 .. 888588
2070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG013500.1

Sequence Viewer

Length: 1038 bp
ATGTCTAAATGCAATTCTTCAGTTGGCAAAACCCCTTCTCTGATCTCCATCGTGTTTCTACTTGGGCAGCTCATGATGATAACCCTACAAACAGCAGATGCCCAAATTGGTGTATGCTATGGAATGCTTGGCAACTTACCACCTCAAAGAGATGTTGTTGCTCTTTACAAACAATACAACATTCGAAGGATGCGACTCTATGATCCAAACCAAGCCGCTCTCGCTGCCCTGAGGGGCTCTAACATCGAGCTCATTCTCGGCGTCCCAAACGACAACCTTCAAACCATTGCCTCAAGCCAGGCCAATGCAAACTCATGGGTTCAAAACAATGTCCGAAACTACGGCGATGTAAGATTCAAGTACATTGCTGTCGGAAACGAAGTCCAGCCAGGAGAATCATCTGCACAGTTTCTAGTCCCGGCCATGCGAAACATTCAAACCGCAATTTCCAATGCTGGTCTTGGAAACCAAATAAAAGTTTCCACAGCCATCGACACCAGAGGACTCGGAGTATCGTACCCTCCCTCAAAAGGTTCTTTCAAGTCTGAAAATAGAGCGCTGCTCGATCCCATTATACGCTTCTTAGTGGAGAATAGATCTCCATTGCTGGTTAACCTGTACCCGTATTTTAGTTACAAGGACAACACCGGTGCCATTCGTCTCGATTATGCTCTCTTCACAGCTCCATCGGTTGTTGTACAAGATGGCCAACTTGGTTATCGTAATCTTTTCGATGCCATGTTGGACGCTGTGTATTCTGCTCTTGAGAAGGCCGGTGGAGGGTCTTTGGAAATTGTTGTATCGGAGAGCGGCTGGCCAACGGCCGGTGGAACAGCAACTACTGTTGACAATGCAAGGACTTACAACTCAAACTTGATTCAACATGTCAAGGGTGGGACTCCGAAAAAGCCCGGGAGGCCGATAGAAACTTACATATTTGCCATGTTCGATGAGGATAGGAAAAATCCAGAGCTTGAGAAACATTGGGGGCTGTTTTCCCCGAACAAACAACCAAAATACCCTATCAATTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

345

Amino Acids

37.84

Weight (kDa)

9.13

Isoelectric Point (pI)

37.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 36 - 344 8.9e-118 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 650
AccBSI CCGCTC 2 cut(s) 218, 810
AciI CCGC 3 cut(s) 216, 441, 810
AclWI GGATC 2 cut(s) 197, 560
AcoI YGGCCR 4 cut(s) 420, 706, 815, 822
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 4 cut(s) 362, 518, 620, 699
AfeI AGCGCT 1 cut(s) 558
AfiI CCNNNNNNNGG 3 cut(s) 530, 780, 824
AflIII ACRYGT 1 cut(s) 883
AgeI ACCGGT 1 cut(s) 647
AgsI TTSAA 7 cut(s) 281, 323, 358, 437, 541, 881, 1033
AjnI CCWGG 2 cut(s) 297, 388
AluBI AGCT 4 cut(s) 70, 250, 683, 973
AluI AGCT 4 cut(s) 70, 250, 683, 973
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 2 cut(s) 197, 560
Ama87I CYCGRG 1 cut(s) 911
Aor51HI AGCGCT 1 cut(s) 558
AoxI GGCC 7 cut(s) 300, 420, 706, 771, 815, 822, 917
ApeKI GCWGC 3 cut(s) 67, 224, 559
AsiGI ACCGGT 1 cut(s) 647
AspLEI GCGC 1 cut(s) 559
AsuC2I CCSGG 3 cut(s) 419, 912, 913
AsuII TTCGAA 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 911
AxyI CCTNAGG 1 cut(s) 230
BalI TGGCCA 2 cut(s) 708, 817
BanI GGYRCC 1 cut(s) 650
BanII GRGCYC 2 cut(s) 239, 252
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 3 cut(s) 79, 211, 546
BccI CCATC 4 cut(s) 56, 497, 694, 698
BceAI ACGGC 2 cut(s) 358, 837
BciT130I CCWGG 2 cut(s) 299, 390
BcnI CCSGG 3 cut(s) 419, 912, 913
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 1 cut(s) 413
BfoI RGCGCY 1 cut(s) 560
BglI GCCNNNNNGGC 1 cut(s) 916
BglII AGATCT 1 cut(s) 596
BisI GCNGC 5 cut(s) 68, 216, 225, 560, 811
BlsI GCNGC 5 cut(s) 69, 217, 226, 561, 812
Bme1390I CCNGG 5 cut(s) 299, 390, 419, 912, 913
BmeT110I CYCGRG 1 cut(s) 911
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 5 cut(s) 299, 390, 419, 912, 913
BmsI GCATC 3 cut(s) 88, 180, 724
BplI GAGNNNNNCTC 2 cut(s) 546, 578
Bpu14I TTCGAA 1 cut(s) 184
BpuEI CTTGAG 3 cut(s) 277, 785, 995
BpuMI CCSGG 3 cut(s) 419, 912, 913
BsaBI GATNNNNATC 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 911
BsaWI WCCGGW 1 cut(s) 647
Bsc4I CCNNNNNNNGG 3 cut(s) 530, 780, 824
Bse118I RCCGGY 3 cut(s) 647, 773, 824
Bse21I CCTNAGG 1 cut(s) 230
Bse3DI GCAATG 3 cut(s) 285, 363, 602
Bse8I GATNNNNATC 1 cut(s) 47
BseBI CCWGG 2 cut(s) 299, 390
BseDI CCNNGG 1 cut(s) 911
BseGI GGATG 1 cut(s) 195
BseJI GATNNNNATC 1 cut(s) 47
BseLI CCNNNNNNNGG 3 cut(s) 530, 780, 824
BseMI GCAATG 3 cut(s) 285, 363, 602
BseMII CTCAG 1 cut(s) 221
BseX3I CGGCCG 1 cut(s) 822
BseXI GCAGC 3 cut(s) 79, 211, 546
BsgI GTGCAG 1 cut(s) 387
Bsh1285I CGRYCG 1 cut(s) 825
BshFI GGCC 7 cut(s) 302, 422, 708, 773, 817, 824, 919
BshNI GGYRCC 1 cut(s) 650
BshTI ACCGGT 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 825
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 911
BsiSI CCGG 5 cut(s) 419, 648, 774, 825, 912
BslFI GGGAC 3 cut(s) 248, 401, 910
BslI CCNNNNNNNGG 3 cut(s) 530, 780, 824
BsmAI GTCTC 1 cut(s) 665
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 3 cut(s) 248, 401, 910
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 7 cut(s) 302, 422, 708, 773, 817, 824, 919
BsoBI CYCGRG 1 cut(s) 911
Bsp119I TTCGAA 1 cut(s) 184
Bsp1286I GDGCHC 2 cut(s) 239, 252
Bsp1407I TGTACA 1 cut(s) 697
Bsp143I GATC 4 cut(s) 42, 202, 565, 596
BspACI CCGC 3 cut(s) 216, 441, 810
BspANI GGCC 7 cut(s) 302, 422, 708, 773, 817, 824, 919
BspCNI CTCAG 1 cut(s) 222
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 652
BspPI GGATC 2 cut(s) 197, 560
BspT104I TTCGAA 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 650
BsrBI CCGCTC 2 cut(s) 218, 810
BsrDI GCAATG 3 cut(s) 285, 363, 602
BsrFI RCCGGY 3 cut(s) 647, 773, 824
BsrGI TGTACA 1 cut(s) 697
BssAI RCCGGY 3 cut(s) 647, 773, 824
BssECI CCNNGG 1 cut(s) 911
BssMI GATC 4 cut(s) 42, 202, 565, 596
BssNI GRCGYC 1 cut(s) 261
Bst2UI CCWGG 2 cut(s) 299, 390
Bst4CI ACNGT 2 cut(s) 408, 844
Bst6I CTCTTC 1 cut(s) 680
BstACI GRCGYC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 815
BstDEI CTNAG 2 cut(s) 230, 583
BstF5I GGATG 1 cut(s) 195
BstH2I RGCGCY 1 cut(s) 560
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 4 cut(s) 45, 205, 568, 599
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 4 cut(s) 42, 202, 565, 596
BstMCI CGRYCG 1 cut(s) 825
BstMWI GCNNNNNNNGC 3 cut(s) 221, 224, 916
BstNI CCWGG 2 cut(s) 299, 390
BstNSI RCATGY 1 cut(s) 887
BstSCI CCNGG 5 cut(s) 297, 388, 417, 910, 911
BstV1I GCAGC 3 cut(s) 79, 211, 546
BstX2I RGATCY 1 cut(s) 596
BstYI RGATCY 1 cut(s) 596
BstZI CGGCCG 1 cut(s) 822
Bsu36I CCTNAGG 1 cut(s) 230
BsuRI GGCC 7 cut(s) 302, 422, 708, 773, 817, 824, 919
BtgZI GCGATG 1 cut(s) 360
BtsCI GGATG 1 cut(s) 195
Cac8I GCNNGC 1 cut(s) 815
CciI TCATGA 1 cut(s) 72
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 3 cut(s) 647, 773, 824
Cfr9I CCCGGG 1 cut(s) 911
CseI GACGC 2 cut(s) 250, 755
Csp6I GTAC 4 cut(s) 361, 517, 619, 698
CspAI ACCGGT 1 cut(s) 647
CviAII CATG 6 cut(s) 73, 315, 424, 739, 884, 943
CviQI GTAC 4 cut(s) 361, 517, 619, 698
DdeI CTNAG 2 cut(s) 230, 583
DpnI GATC 4 cut(s) 44, 204, 567, 598
DpnII GATC 4 cut(s) 42, 202, 565, 596
EaeI YGGCCR 4 cut(s) 420, 706, 815, 822
EagI CGGCCG 1 cut(s) 822
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
Ecl136II GAGCTC 1 cut(s) 250
EclXI CGGCCG 1 cut(s) 822
Eco24I GRGCYC 2 cut(s) 239, 252
Eco47III AGCGCT 1 cut(s) 558
Eco52I CGGCCG 1 cut(s) 822
Eco53kI GAGCTC 1 cut(s) 250
Eco81I CCTNAGG 1 cut(s) 230
Eco88I CYCGRG 1 cut(s) 911
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 2 cut(s) 297, 388
EcoT38I GRGCYC 2 cut(s) 239, 252
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 6 cut(s) 76, 318, 427, 742, 887, 946
FaqI GGGAC 3 cut(s) 248, 401, 910
FatI CATG 6 cut(s) 72, 314, 423, 738, 883, 942
Fnu4HI GCNGC 5 cut(s) 68, 216, 225, 560, 811
FokI GGATG 1 cut(s) 202
FriOI GRGCYC 2 cut(s) 239, 252
Fsp4HI GCNGC 5 cut(s) 68, 216, 225, 560, 811
FspBI CTAG 1 cut(s) 413
GlaI GCGC 1 cut(s) 558
GluI GCNGC 5 cut(s) 68, 216, 225, 560, 811
HaeII RGCGCY 1 cut(s) 560
HaeIII GGCC 7 cut(s) 302, 422, 708, 773, 817, 824, 919
HapII CCGG 5 cut(s) 419, 648, 774, 825, 912
HgaI GACGC 2 cut(s) 250, 755
HhaI GCGC 1 cut(s) 559
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 6 cut(s) 76, 318, 427, 742, 887, 946
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 2 cut(s) 613, 847
HindII GTYRAC 2 cut(s) 613, 847
HinfI GANTC 6 cut(s) 195, 354, 395, 504, 877, 898
HpaI GTTAAC 1 cut(s) 613
HpaII CCGG 5 cut(s) 419, 648, 774, 825, 912
Hpy166II GTNNAC 2 cut(s) 613, 847
Hpy188I TCNGA 7 cut(s) 42, 335, 374, 509, 547, 805, 903
Hpy188III TCNNGA 4 cut(s) 73, 662, 764, 968
Hpy8I GTNNAC 2 cut(s) 613, 847
HpyAV CCTTC 4 cut(s) 45, 180, 287, 763
HpyCH4III ACNGT 2 cut(s) 408, 844
HpyCH4V TGCA 4 cut(s) 12, 308, 404, 854
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 224, 916
HpyF3I CTNAG 2 cut(s) 230, 583
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 6 cut(s) 76, 318, 427, 742, 887, 946
HspAI GCGC 1 cut(s) 557
KspAI GTTAAC 1 cut(s) 613
Kzo9I GATC 4 cut(s) 42, 202, 565, 596
LmnI GCTCC 1 cut(s) 688
Lsp1109I GCAGC 3 cut(s) 79, 211, 546
LweI GCATC 3 cut(s) 88, 180, 724
MaeI CTAG 1 cut(s) 413
MaeIII GTNAC 1 cut(s) 632
MalI GATC 4 cut(s) 44, 204, 567, 598
MbiI CCGCTC 2 cut(s) 218, 810
MboI GATC 4 cut(s) 42, 202, 565, 596
MboII GAAGA 2 cut(s) 9, 667
MfeI CAATTG 1 cut(s) 1033
MflI RGATCY 1 cut(s) 596
MhlI GDGCHC 2 cut(s) 239, 252
MlsI TGGCCA 2 cut(s) 708, 817
MluCI AATT 6 cut(s) 13, 105, 444, 792, 1027, 1033
MluNI TGGCCA 2 cut(s) 708, 817
MlyI GAGTC 3 cut(s) 189, 498, 892
MmeI TCCRAC 2 cut(s) 352, 723
MnlI CCTC 9 cut(s) 153, 225, 301, 494, 531, 535, 773, 909, 946
Mox20I TGGCCA 2 cut(s) 708, 817
MscI TGGCCA 2 cut(s) 708, 817
MseI TTAA 1 cut(s) 612
Msp20I TGGCCA 2 cut(s) 708, 817
MspI CCGG 5 cut(s) 419, 648, 774, 825, 912
MspR9I CCNGG 5 cut(s) 299, 390, 419, 912, 913
MunI CAATTG 1 cut(s) 1033
Mva1269I GAATGC 1 cut(s) 129
MvaI CCWGG 2 cut(s) 299, 390
MwoI GCNNNNNNNGC 3 cut(s) 221, 224, 916
NciI CCSGG 3 cut(s) 419, 912, 913
NdeII GATC 4 cut(s) 42, 202, 565, 596
NlaIII CATG 6 cut(s) 76, 318, 427, 742, 887, 946
NlaIV GGNNCC 1 cut(s) 652
NmeAIII GCCGAG 1 cut(s) 237
NspI RCATGY 1 cut(s) 887
NspV TTCGAA 1 cut(s) 184
PagI TCATGA 1 cut(s) 72
PciI ACATGT 1 cut(s) 883
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PctI GAATGC 1 cut(s) 129
PfeI GAWTC 3 cut(s) 354, 395, 877
PinAI ACCGGT 1 cut(s) 647
PkrI GCNGC 5 cut(s) 69, 217, 226, 561, 812
PleI GAGTC 3 cut(s) 189, 498, 892
PpsI GAGTC 3 cut(s) 189, 498, 892
PscI ACATGT 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 297, 388
PspGI CCWGG 2 cut(s) 297, 388
PspN4I GGNNCC 1 cut(s) 652
PsrI GAACNNNNNNTAC 2 cut(s) 823, 855
PsuI RGATCY 1 cut(s) 596
RsaI GTAC 4 cut(s) 362, 518, 620, 699
RsaNI GTAC 4 cut(s) 361, 517, 619, 698
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 5 cut(s) 68, 216, 225, 560, 811
Sau3AI GATC 4 cut(s) 42, 202, 565, 596
SchI GAGTC 3 cut(s) 189, 498, 892
ScrFI CCNGG 5 cut(s) 299, 390, 419, 912, 913
SduI GDGCHC 2 cut(s) 239, 252
SetI ASST 8 cut(s) 72, 145, 252, 279, 535, 618, 685, 975
SfaNI GCATC 3 cut(s) 88, 180, 724
SfuI TTCGAA 1 cut(s) 184
SgrAI CRCCGGYG 1 cut(s) 647
SmaI CCCGGG 1 cut(s) 913
SmlI CTYRAG 3 cut(s) 292, 764, 974
SmoI CTYRAG 3 cut(s) 292, 764, 974
Sse9I AATT 6 cut(s) 13, 105, 444, 792, 1027, 1033
SsiI CCGC 3 cut(s) 216, 441, 810
SspMI CTAG 1 cut(s) 413
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 5 cut(s) 297, 388, 417, 910, 911
TaaI ACNGT 2 cut(s) 408, 844
TaqI TCGA 7 cut(s) 184, 246, 492, 564, 663, 732, 948
TasI AATT 6 cut(s) 13, 105, 444, 792, 1027, 1033
TatI WGTACW 2 cut(s) 360, 697
TauI GCSGC 2 cut(s) 218, 813
TfiI GAWTC 3 cut(s) 354, 395, 877
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TseI GCWGC 3 cut(s) 67, 224, 559
TspMI CCCGGG 1 cut(s) 911
XceI RCATGY 1 cut(s) 887
XmaI CCCGGG 1 cut(s) 911
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.