pycom14g06890

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006449
Physical Location & Seq
Forward (+)
6868752 .. 6869676
925 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06890.1

Sequence Viewer

Length: 879 bp
ATGAATGGAGACTTACCACCCCAAGCAGAAGTTATTGCTCTCTACAAGCAAAATAACATCCGAAGAATGCGACTATATGATCCGAACCCAGCAGCTCTAGCAGCCCTCAGAGGCTCTGACATTGAGCTCATGTTAGACATACCAAAGGACCAACTTCAAAACATTGCTTCAAGCCAAGCCAATGCAAATACATGGATCCAAAACAATGTCAGAAACTATGCCAATGTGAGATTCAAATACATTGCCGTAGGAAACGAAGTCACGCCCTCGGACTCATCTGCGCAATTTCTGGTCCCGGCCATGCGGAACATTCAAAATGCGATTTCCAGTGCCGGTCTTGGAAATCAAATCAAAGTTTCCACCGCCATAGACACCGGTGTGCTTGGGAATTCCTTTCCTCCATCAAACGGAGAATTTAGGGGTGACTATAGCCCAATTTTGAATCCTGTTGTCCAGTTCCTGGTGGACAACAAATCTCCGCTGCTCGTTAATTTATATCCTTATTTTAGTTATATTGGCAACACTCATGACATTCGTCTAGATTATGCTCTTTTCACAGCTCCGTCAGTTGTAGTACAAGATGGCCAACGTGGTTATCGTAATCTTTTCGATGCCATTTTGGATGCTGTTTACGCTGCGCTTGACAAGAGTGGTTGGCCGACAGCTGGCGGGACGGCAACAACAGTTGATAATGCGAGGACTTATAACTCCAATTTGATTCAACATGTGAAGGGAGGGACTCCAAGGAAGCCTGGAAAGCCCATTGAAACTTACATCTTTGCCATGTTTGATGAGAATAGAAAGACCCCAGAGCTTGAGAAACATTGGGGGCTATTCTCCCCTAACAAACAGCCTAAATACCCAATCAATTTCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

293

Amino Acids

32.34

Weight (kDa)

8.6

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 3 - 291 8.2e-102 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
Acc16I TGCGCA 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 460
AciI CCGC 4 cut(s) 304, 363, 479, 669
AclWI GGATC 3 cut(s) 74, 190, 203
AcoI YGGCCR 3 cut(s) 297, 583, 656
AcsI RAATTY 2 cut(s) 388, 413
AfaI GTAC 1 cut(s) 576
AfiI CCNNNNNNNGG 3 cut(s) 407, 460, 665
AflIII ACRYGT 1 cut(s) 724
AgeI ACCGGT 1 cut(s) 374
AgsI TTSAA 8 cut(s) 158, 171, 235, 314, 442, 722, 767, 874
AjnI CCWGG 2 cut(s) 459, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 5 cut(s) 95, 127, 560, 665, 814
AluI AGCT 5 cut(s) 95, 127, 560, 665, 814
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 1 cut(s) 3
AlwI GGATC 3 cut(s) 74, 190, 203
AlwNI CAGNNNCTG 1 cut(s) 460
AoxI GGCC 3 cut(s) 297, 583, 656
ApeKI GCWGC 4 cut(s) 92, 101, 481, 635
ApoI RAATTY 2 cut(s) 388, 413
AsiGI ACCGGT 1 cut(s) 374
AspLEI GCGC 2 cut(s) 283, 640
AspS9I GGNCC 2 cut(s) 148, 292
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 2 cut(s) 148, 292
BalI TGGCCA 1 cut(s) 585
BamHI GGATCC 1 cut(s) 195
BanII GRGCYC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 4 cut(s) 104, 113, 468, 622
BccI CCATC 2 cut(s) 409, 575
BceAI ACGGC 2 cut(s) 230, 690
BciT130I CCWGG 2 cut(s) 461, 753
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 3
BfaI CTAG 2 cut(s) 98, 539
BfmI CTRYAG 1 cut(s) 427
BisI GCNGC 4 cut(s) 93, 102, 482, 636
BlsI GCNGC 4 cut(s) 94, 103, 483, 637
Bme1390I CCNGG 3 cut(s) 296, 461, 753
Bme18I GGWCC 2 cut(s) 148, 292
BmgT120I GGNCC 2 cut(s) 148, 292
BmiI GGNNCC 2 cut(s) 197, 294
BmrFI CCNGG 3 cut(s) 296, 461, 753
BmsI GCATC 2 cut(s) 601, 613
BoxI GACNNNNGTC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 836
BpuMI CCSGG 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 267, 743
BsaWI WCCGGW 1 cut(s) 374
Bsc4I CCNNNNNNNGG 3 cut(s) 407, 460, 665
Bse118I RCCGGY 2 cut(s) 332, 374
Bse1I ACTGG 2 cut(s) 327, 454
Bse3DI GCAATG 2 cut(s) 162, 240
BseBI CCWGG 2 cut(s) 461, 753
BseDI CCNNGG 2 cut(s) 267, 743
BseGI GGATG 2 cut(s) 57, 628
BseLI CCNNNNNNNGG 3 cut(s) 407, 460, 665
BseMI GCAATG 2 cut(s) 162, 240
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 2 cut(s) 327, 454
BseXI GCAGC 4 cut(s) 104, 113, 468, 622
BseYI CCCAGC 1 cut(s) 88
BshFI GGCC 3 cut(s) 299, 585, 658
BshTI ACCGGT 1 cut(s) 374
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 3 cut(s) 296, 333, 375
BslFI GGGAC 3 cut(s) 278, 685, 751
BslI CCNNNNNNNGG 3 cut(s) 407, 460, 665
BsmAI GTCTC 1 cut(s) 3
BsmFI GGGAC 3 cut(s) 278, 685, 751
BsmI GAATGC 1 cut(s) 72
BsnI GGCC 3 cut(s) 299, 585, 658
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 2 cut(s) 79, 195
BspACI CCGC 4 cut(s) 304, 363, 479, 669
BspANI GGCC 3 cut(s) 299, 585, 658
BspCNI CTCAG 1 cut(s) 120
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 197, 294
BspPI GGATC 3 cut(s) 74, 190, 203
BsrDI GCAATG 2 cut(s) 162, 240
BsrFI RCCGGY 2 cut(s) 332, 374
BsrI ACTGG 2 cut(s) 327, 454
BssAI RCCGGY 2 cut(s) 332, 374
BssECI CCNNGG 2 cut(s) 267, 743
BssMI GATC 2 cut(s) 79, 195
BssT1I CCWWGG 1 cut(s) 743
Bst2UI CCWGG 2 cut(s) 461, 753
Bst4CI ACNGT 1 cut(s) 685
BstC8I GCNNGC 1 cut(s) 667
BstDEI CTNAG 1 cut(s) 107
BstF5I GGATG 2 cut(s) 57, 628
BstHHI GCGC 2 cut(s) 283, 640
BstKTI GATC 2 cut(s) 82, 198
BstMAI GTCTC 1 cut(s) 3
BstMBI GATC 2 cut(s) 79, 195
BstMWI GCNNNNNNNGC 4 cut(s) 98, 101, 632, 757
BstNI CCWGG 2 cut(s) 461, 753
BstNSI RCATGY 1 cut(s) 728
BstPAI GACNNNNGTC 1 cut(s) 534
BstSCI CCNGG 3 cut(s) 294, 459, 751
BstSFI CTRYAG 1 cut(s) 427
BstV1I GCAGC 4 cut(s) 104, 113, 468, 622
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
BsuRI GGCC 3 cut(s) 299, 585, 658
BtsCI GGATG 2 cut(s) 57, 628
BtsIMutI CAGTG 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 667
CaiI CAGNNNCTG 1 cut(s) 460
CciI TCATGA 1 cut(s) 526
CfoI GCGC 2 cut(s) 283, 640
Cfr10I RCCGGY 2 cut(s) 332, 374
Cfr13I GGNCC 2 cut(s) 148, 292
Csp6I GTAC 1 cut(s) 575
CspAI ACCGGT 1 cut(s) 374
CviAII CATG 6 cut(s) 130, 192, 301, 527, 725, 784
CviQI GTAC 1 cut(s) 575
DdeI CTNAG 1 cut(s) 107
DpnI GATC 2 cut(s) 81, 197
DpnII GATC 2 cut(s) 79, 195
EaeI YGGCCR 3 cut(s) 297, 583, 656
Ecl136II GAGCTC 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 2 cut(s) 148, 292
Eco53kI GAGCTC 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 459, 751
EcoT14I CCWWGG 1 cut(s) 743
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 743
FaeI CATG 6 cut(s) 133, 195, 304, 530, 728, 787
FaqI GGGAC 3 cut(s) 278, 685, 751
FatI CATG 6 cut(s) 129, 191, 300, 526, 724, 783
FauI CCCGC 1 cut(s) 662
Fnu4HI GCNGC 4 cut(s) 93, 102, 482, 636
FokI GGATG 2 cut(s) 44, 635
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 4 cut(s) 93, 102, 482, 636
FspBI CTAG 2 cut(s) 98, 539
FspI TGCGCA 1 cut(s) 282
GlaI GCGC 2 cut(s) 282, 639
GluI GCNGC 4 cut(s) 93, 102, 482, 636
GsaI CCCAGC 1 cut(s) 92
HaeIII GGCC 3 cut(s) 299, 585, 658
HapII CCGG 3 cut(s) 296, 333, 375
HhaI GCGC 2 cut(s) 283, 640
Hin1II CATG 6 cut(s) 133, 195, 304, 530, 728, 787
Hin6I GCGC 2 cut(s) 281, 638
HinP1I GCGC 2 cut(s) 281, 638
HinfI GANTC 5 cut(s) 231, 272, 442, 718, 739
HpaII CCGG 3 cut(s) 296, 333, 375
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 466, 631
Hpy188I TCNGA 6 cut(s) 62, 84, 110, 118, 212, 271
Hpy188III TCNNGA 2 cut(s) 527, 539
Hpy8I GTNNAC 2 cut(s) 466, 631
HpyAV CCTTC 1 cut(s) 724
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4IV ACGT 1 cut(s) 589
HpyCH4V TGCA 1 cut(s) 185
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 101, 632, 757
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 1 cut(s) 589
Hsp92II CATG 6 cut(s) 133, 195, 304, 530, 728, 787
HspAI GCGC 2 cut(s) 281, 638
Kzo9I GATC 2 cut(s) 79, 195
LmnI GCTCC 1 cut(s) 565
Lsp1109I GCAGC 4 cut(s) 104, 113, 468, 622
LweI GCATC 2 cut(s) 601, 613
MaeI CTAG 2 cut(s) 98, 539
MaeII ACGT 1 cut(s) 589
MaeIII GTNAC 2 cut(s) 259, 422
MalI GATC 2 cut(s) 81, 197
MboI GATC 2 cut(s) 79, 195
MboII GAAGA 1 cut(s) 75
MflI RGATCY 1 cut(s) 195
MhlI GDGCHC 1 cut(s) 129
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 7 cut(s) 284, 388, 413, 435, 490, 712, 868
MluNI TGGCCA 1 cut(s) 585
MlyI GAGTC 2 cut(s) 266, 733
MnlI CCTC 6 cut(s) 104, 116, 277, 408, 690, 728
Mox20I TGGCCA 1 cut(s) 585
MscI TGGCCA 1 cut(s) 585
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 585
MspA1I CMGCKG 2 cut(s) 481, 665
MspI CCGG 3 cut(s) 296, 333, 375
MspR9I CCNGG 3 cut(s) 296, 461, 753
Mva1269I GAATGC 1 cut(s) 72
MvaI CCWGG 2 cut(s) 461, 753
MwoI GCNNNNNNNGC 4 cut(s) 98, 101, 632, 757
NciI CCSGG 1 cut(s) 296
NdeII GATC 2 cut(s) 79, 195
NlaIII CATG 6 cut(s) 133, 195, 304, 530, 728, 787
NlaIV GGNNCC 2 cut(s) 197, 294
NmuCI GTSAC 2 cut(s) 259, 422
NsbI TGCGCA 1 cut(s) 282
NspI RCATGY 1 cut(s) 728
OliI CACNNNNGTG 1 cut(s) 377
PagI TCATGA 1 cut(s) 526
PciI ACATGT 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PctI GAATGC 1 cut(s) 72
PfeI GAWTC 3 cut(s) 231, 442, 718
PflMI CCANNNNNTGG 1 cut(s) 460
PinAI ACCGGT 1 cut(s) 374
PkrI GCNGC 4 cut(s) 94, 103, 483, 637
PleI GAGTC 2 cut(s) 266, 733
PpsI GAGTC 2 cut(s) 266, 733
PscI ACATGT 1 cut(s) 724
PshAI GACNNNNGTC 1 cut(s) 534
PsiI TTATAA 1 cut(s) 705
Psp124BI GAGCTC 1 cut(s) 129
Psp6I CCWGG 2 cut(s) 459, 751
PspFI CCCAGC 1 cut(s) 88
PspGI CCWGG 2 cut(s) 459, 751
PspN4I GGNNCC 2 cut(s) 197, 294
PspPI GGNCC 2 cut(s) 148, 292
PstNI CAGNNNCTG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 665
RsaI GTAC 1 cut(s) 576
RsaNI GTAC 1 cut(s) 575
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 129
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 4 cut(s) 93, 102, 482, 636
Sau3AI GATC 2 cut(s) 79, 195
Sau96I GGNCC 2 cut(s) 148, 292
SchI GAGTC 2 cut(s) 266, 733
ScrFI CCNGG 3 cut(s) 296, 461, 753
SduI GDGCHC 1 cut(s) 129
SetI ASST 6 cut(s) 97, 129, 562, 592, 667, 816
SfaNI GCATC 2 cut(s) 601, 613
SfcI CTRYAG 1 cut(s) 427
SgrAI CRCCGGYG 1 cut(s) 374
SinI GGWCC 2 cut(s) 148, 292
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
Sse9I AATT 7 cut(s) 284, 388, 413, 435, 490, 712, 868
SsiI CCGC 4 cut(s) 304, 363, 479, 669
SspMI CTAG 2 cut(s) 98, 539
SstI GAGCTC 1 cut(s) 129
StyD4I CCNGG 3 cut(s) 294, 459, 751
StyI CCWWGG 1 cut(s) 743
TaaI ACNGT 1 cut(s) 685
TaiI ACGT 1 cut(s) 592
TaqI TCGA 1 cut(s) 609
TasI AATT 7 cut(s) 284, 388, 413, 435, 490, 712, 868
TatI WGTACW 1 cut(s) 574
TfiI GAWTC 3 cut(s) 231, 442, 718
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 259, 422
TseI GCWGC 4 cut(s) 92, 101, 481, 635
Tsp45I GTSAC 2 cut(s) 259, 422
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 423, 552
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 148, 292
XapI RAATTY 2 cut(s) 388, 413
XbaI TCTAGA 1 cut(s) 538
XceI RCATGY 1 cut(s) 728
XspI CTAG 2 cut(s) 98, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.