Rw7G001070

Glucan endo-1,3-beta-glucosidase, basic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006449
Physical Location & Seq
Reverse (-)
885457 .. 887253
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001070.1

Sequence Viewer

Length: 1056 bp
ATGTGCTATCTTTTGATCATGTCTAAATGCAATTCTTCAGTTGGCAAAACCCCTTCTCTGATCTCCATCGTGTTTCTACTTGGGCAGCTCATGATGATAACCCTACAAACAGCAGATGCCCAAATTGGTGTATGCTATGGAATGCTTGGCAACTTACCACCTCAAAGAGATGTTGTTGCTCTTTACAAACAATACAACATTCGAAGGATGCGACTCTATGATCCAAACCAAGCCGCTCTCGCTGCCCTGAGGGGCTCTAACATCGAGCTCATTCTCGGCGTCCCAAACGACAACCTTCAAACCATTGCCTCAAGCCAGGCCAATGCAAACTCATGGGTTCAAAACAATGTCCGAAACTACGGCGATGTAAGATTCAAGTACATTGCTGTCGGAAACGAAGTCCAGCCAGGAGAATCATCTGCACAGTTTCTAGTCCCGGCCATGCGAAACATTCAAACCGCAATTTCCAATGCTGGTCTTGGAAACCAAATCAAAGTTTCCACAGCCATCGACACCAGAGGACTCGGAGTATCGTACCCTCCCTCAAAAGGTTCTTTCAAGTCTGAAAATAGAGCGCTGCTCGATCCCATTATACGCTTCTTAGTGGAGAATAGATCTCCATTGCTGGTTAACCTGTACCCGTATTTTAGTTACAAGGACAACACCGGTGCCATTCGTCTCGATTATGCTCTCTTCACAGCTCCATCGGTTGTTGTACAAGATGGCCAACTTGGTTATCGTAATCTTTTCGATGCCATGTTGGACGCTGTGTATTCTGCTCTTGAGAAGGCCGGTGGAGGGTCTTTGGAAATTGTTGTATCGGAGAGCGGCTGGCCAACGGCCGGTGGAACAGCAACTAGTGTTGACAATGCAAGGACTTACAACTCAAACTTGATTCAACATGTCAAGGGTGGGACTCCGAAAAAGCCCGGGAGGCCGATAGAAACTTACATATTTGCCATGTTCGATGAGGATAGGAAAAATCCAGAGCTTGAGAAACATTGGGGGCTGTTTTCCCCGAACAAACAACCAAAATACCCTATCAATTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17

Protein Analysis

351

Amino Acids

38.56

Weight (kDa)

9.06

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 42 - 350 1e-117 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 668
AccBSI CCGCTC 2 cut(s) 236, 828
AciI CCGC 3 cut(s) 234, 459, 828
AclWI GGATC 2 cut(s) 215, 578
AcoI YGGCCR 4 cut(s) 438, 724, 833, 840
AcuI CTGAAG 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 380, 536, 638, 717
AfeI AGCGCT 1 cut(s) 576
AfiI CCNNNNNNNGG 3 cut(s) 548, 798, 842
AflIII ACRYGT 1 cut(s) 901
AgeI ACCGGT 1 cut(s) 665
AgsI TTSAA 7 cut(s) 299, 341, 376, 455, 559, 899, 1051
AhlI ACTAGT 1 cut(s) 857
AjnI CCWGG 2 cut(s) 315, 406
AluBI AGCT 4 cut(s) 88, 268, 701, 991
AluI AGCT 4 cut(s) 88, 268, 701, 991
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 2 cut(s) 215, 578
Ama87I CYCGRG 1 cut(s) 929
Aor51HI AGCGCT 1 cut(s) 576
AoxI GGCC 7 cut(s) 318, 438, 724, 789, 833, 840, 935
ApeKI GCWGC 3 cut(s) 85, 242, 577
AsiGI ACCGGT 1 cut(s) 665
AspLEI GCGC 1 cut(s) 577
AsuC2I CCSGG 3 cut(s) 437, 930, 931
AsuII TTCGAA 1 cut(s) 202
AvaI CYCGRG 1 cut(s) 929
AxyI CCTNAGG 1 cut(s) 248
BalI TGGCCA 2 cut(s) 726, 835
BanI GGYRCC 1 cut(s) 668
BanII GRGCYC 2 cut(s) 257, 270
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 97, 229, 564
BccI CCATC 4 cut(s) 74, 515, 712, 716
BceAI ACGGC 2 cut(s) 376, 855
BciT130I CCWGG 2 cut(s) 317, 408
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 3 cut(s) 437, 930, 931
BcoDI GTCTC 1 cut(s) 683
BcuI ACTAGT 1 cut(s) 857
BfaI CTAG 2 cut(s) 431, 858
BfoI RGCGCY 1 cut(s) 578
BglI GCCNNNNNGGC 1 cut(s) 934
BglII AGATCT 1 cut(s) 614
BisI GCNGC 5 cut(s) 86, 234, 243, 578, 829
BlsI GCNGC 5 cut(s) 87, 235, 244, 579, 830
Bme1390I CCNGG 5 cut(s) 317, 408, 437, 930, 931
BmeT110I CYCGRG 1 cut(s) 929
BmiI GGNNCC 1 cut(s) 670
BmrFI CCNGG 5 cut(s) 317, 408, 437, 930, 931
BmsI GCATC 3 cut(s) 106, 198, 742
BplI GAGNNNNNCTC 2 cut(s) 564, 596
Bpu14I TTCGAA 1 cut(s) 202
BpuEI CTTGAG 3 cut(s) 295, 803, 1013
BpuMI CCSGG 3 cut(s) 437, 930, 931
BsaBI GATNNNNATC 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 1 cut(s) 929
BsaWI WCCGGW 1 cut(s) 665
Bsc4I CCNNNNNNNGG 3 cut(s) 548, 798, 842
Bse118I RCCGGY 3 cut(s) 665, 791, 842
Bse21I CCTNAGG 1 cut(s) 248
Bse3DI GCAATG 3 cut(s) 303, 381, 620
Bse8I GATNNNNATC 1 cut(s) 65
BseBI CCWGG 2 cut(s) 317, 408
BseDI CCNNGG 1 cut(s) 929
BseGI GGATG 1 cut(s) 213
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 3 cut(s) 548, 798, 842
BseMI GCAATG 3 cut(s) 303, 381, 620
BseMII CTCAG 1 cut(s) 239
BseX3I CGGCCG 1 cut(s) 840
BseXI GCAGC 3 cut(s) 97, 229, 564
BsgI GTGCAG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 843
BshFI GGCC 7 cut(s) 320, 440, 726, 791, 835, 842, 937
BshNI GGYRCC 1 cut(s) 668
BshTI ACCGGT 1 cut(s) 665
BsiEI CGRYCG 1 cut(s) 843
BsiHKAI GWGCWC 1 cut(s) 270
BsiHKCI CYCGRG 1 cut(s) 929
BsiSI CCGG 5 cut(s) 437, 666, 792, 843, 930
BslFI GGGAC 3 cut(s) 266, 419, 928
BslI CCNNNNNNNGG 3 cut(s) 548, 798, 842
BsmAI GTCTC 1 cut(s) 683
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 3 cut(s) 266, 419, 928
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 7 cut(s) 320, 440, 726, 791, 835, 842, 937
BsoBI CYCGRG 1 cut(s) 929
Bsp119I TTCGAA 1 cut(s) 202
Bsp1286I GDGCHC 2 cut(s) 257, 270
Bsp1407I TGTACA 1 cut(s) 715
Bsp143I GATC 5 cut(s) 15, 60, 220, 583, 614
BspACI CCGC 3 cut(s) 234, 459, 828
BspANI GGCC 7 cut(s) 320, 440, 726, 791, 835, 842, 937
BspCNI CTCAG 1 cut(s) 240
BspHI TCATGA 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 670
BspPI GGATC 2 cut(s) 215, 578
BspT104I TTCGAA 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 668
BsrBI CCGCTC 2 cut(s) 236, 828
BsrDI GCAATG 3 cut(s) 303, 381, 620
BsrFI RCCGGY 3 cut(s) 665, 791, 842
BsrGI TGTACA 1 cut(s) 715
BssAI RCCGGY 3 cut(s) 665, 791, 842
BssECI CCNNGG 1 cut(s) 929
BssMI GATC 5 cut(s) 15, 60, 220, 583, 614
BssNI GRCGYC 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 317, 408
Bst4CI ACNGT 1 cut(s) 426
Bst6I CTCTTC 1 cut(s) 698
BstACI GRCGYC 1 cut(s) 279
BstAUI TGTACA 1 cut(s) 715
BstBI TTCGAA 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 833
BstDEI CTNAG 2 cut(s) 248, 601
BstF5I GGATG 1 cut(s) 213
BstH2I RGCGCY 1 cut(s) 578
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 5 cut(s) 18, 63, 223, 586, 617
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 5 cut(s) 15, 60, 220, 583, 614
BstMCI CGRYCG 1 cut(s) 843
BstMWI GCNNNNNNNGC 3 cut(s) 239, 242, 934
BstNI CCWGG 2 cut(s) 317, 408
BstNSI RCATGY 1 cut(s) 905
BstSCI CCNGG 5 cut(s) 315, 406, 435, 928, 929
BstV1I GCAGC 3 cut(s) 97, 229, 564
BstX2I RGATCY 1 cut(s) 614
BstYI RGATCY 1 cut(s) 614
BstZI CGGCCG 1 cut(s) 840
Bsu36I CCTNAGG 1 cut(s) 248
BsuRI GGCC 7 cut(s) 320, 440, 726, 791, 835, 842, 937
BtgZI GCGATG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 833
CciI TCATGA 1 cut(s) 90
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 3 cut(s) 665, 791, 842
Cfr9I CCCGGG 1 cut(s) 929
CseI GACGC 2 cut(s) 268, 773
Csp6I GTAC 4 cut(s) 379, 535, 637, 716
CspAI ACCGGT 1 cut(s) 665
CviAII CATG 7 cut(s) 19, 91, 333, 442, 757, 902, 961
CviQI GTAC 4 cut(s) 379, 535, 637, 716
DdeI CTNAG 2 cut(s) 248, 601
DpnI GATC 5 cut(s) 17, 62, 222, 585, 616
DpnII GATC 5 cut(s) 15, 60, 220, 583, 614
EaeI YGGCCR 4 cut(s) 438, 724, 833, 840
EagI CGGCCG 1 cut(s) 840
Eam1104I CTCTTC 1 cut(s) 698
EarI CTCTTC 1 cut(s) 698
Ecl136II GAGCTC 1 cut(s) 268
EclXI CGGCCG 1 cut(s) 840
Eco24I GRGCYC 2 cut(s) 257, 270
Eco47III AGCGCT 1 cut(s) 576
Eco52I CGGCCG 1 cut(s) 840
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 21
Eco81I CCTNAGG 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 929
EcoICRI GAGCTC 1 cut(s) 268
EcoRII CCWGG 2 cut(s) 315, 406
EcoT38I GRGCYC 2 cut(s) 257, 270
Esp3I CGTCTC 1 cut(s) 683
FaeI CATG 7 cut(s) 22, 94, 336, 445, 760, 905, 964
FaqI GGGAC 3 cut(s) 266, 419, 928
FatI CATG 7 cut(s) 18, 90, 332, 441, 756, 901, 960
FbaI TGATCA 1 cut(s) 15
Fnu4HI GCNGC 5 cut(s) 86, 234, 243, 578, 829
FokI GGATG 1 cut(s) 220
FriOI GRGCYC 2 cut(s) 257, 270
Fsp4HI GCNGC 5 cut(s) 86, 234, 243, 578, 829
FspBI CTAG 2 cut(s) 431, 858
GlaI GCGC 1 cut(s) 576
GluI GCNGC 5 cut(s) 86, 234, 243, 578, 829
HaeII RGCGCY 1 cut(s) 578
HaeIII GGCC 7 cut(s) 320, 440, 726, 791, 835, 842, 937
HapII CCGG 5 cut(s) 437, 666, 792, 843, 930
HgaI GACGC 2 cut(s) 268, 773
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 7 cut(s) 22, 94, 336, 445, 760, 905, 964
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HincII GTYRAC 2 cut(s) 631, 865
HindII GTYRAC 2 cut(s) 631, 865
HinfI GANTC 6 cut(s) 213, 372, 413, 522, 895, 916
HpaI GTTAAC 1 cut(s) 631
HpaII CCGG 5 cut(s) 437, 666, 792, 843, 930
Hpy166II GTNNAC 2 cut(s) 631, 865
Hpy188I TCNGA 7 cut(s) 60, 353, 392, 527, 565, 823, 921
Hpy188III TCNNGA 4 cut(s) 91, 680, 782, 986
Hpy8I GTNNAC 2 cut(s) 631, 865
HpyAV CCTTC 4 cut(s) 63, 198, 305, 781
HpyCH4III ACNGT 1 cut(s) 426
HpyCH4V TGCA 4 cut(s) 30, 326, 422, 872
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 242, 934
HpyF3I CTNAG 2 cut(s) 248, 601
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 7 cut(s) 22, 94, 336, 445, 760, 905, 964
HspAI GCGC 1 cut(s) 575
Ksp22I TGATCA 1 cut(s) 15
KspAI GTTAAC 1 cut(s) 631
Kzo9I GATC 5 cut(s) 15, 60, 220, 583, 614
LmnI GCTCC 1 cut(s) 706
Lsp1109I GCAGC 3 cut(s) 97, 229, 564
LweI GCATC 3 cut(s) 106, 198, 742
MaeI CTAG 2 cut(s) 431, 858
MaeIII GTNAC 1 cut(s) 650
MalI GATC 5 cut(s) 17, 62, 222, 585, 616
MbiI CCGCTC 2 cut(s) 236, 828
MboI GATC 5 cut(s) 15, 60, 220, 583, 614
MboII GAAGA 2 cut(s) 27, 685
MfeI CAATTG 1 cut(s) 1051
MflI RGATCY 1 cut(s) 614
MhlI GDGCHC 2 cut(s) 257, 270
MlsI TGGCCA 2 cut(s) 726, 835
MluCI AATT 6 cut(s) 31, 123, 462, 810, 1045, 1051
MluNI TGGCCA 2 cut(s) 726, 835
MlyI GAGTC 3 cut(s) 207, 516, 910
MmeI TCCRAC 2 cut(s) 370, 741
MnlI CCTC 9 cut(s) 171, 243, 319, 512, 549, 553, 791, 927, 964
Mox20I TGGCCA 2 cut(s) 726, 835
MscI TGGCCA 2 cut(s) 726, 835
MseI TTAA 1 cut(s) 630
Msp20I TGGCCA 2 cut(s) 726, 835
MspI CCGG 5 cut(s) 437, 666, 792, 843, 930
MspR9I CCNGG 5 cut(s) 317, 408, 437, 930, 931
MunI CAATTG 1 cut(s) 1051
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 2 cut(s) 317, 408
MwoI GCNNNNNNNGC 3 cut(s) 239, 242, 934
NciI CCSGG 3 cut(s) 437, 930, 931
NdeII GATC 5 cut(s) 15, 60, 220, 583, 614
NlaIII CATG 7 cut(s) 22, 94, 336, 445, 760, 905, 964
NlaIV GGNNCC 1 cut(s) 670
NmeAIII GCCGAG 1 cut(s) 255
NspI RCATGY 1 cut(s) 905
NspV TTCGAA 1 cut(s) 202
PagI TCATGA 1 cut(s) 90
PciI ACATGT 1 cut(s) 901
PcsI WCGNNNNNNNCGW 1 cut(s) 208
PctI GAATGC 1 cut(s) 147
PfeI GAWTC 3 cut(s) 372, 413, 895
PinAI ACCGGT 1 cut(s) 665
PkrI GCNGC 5 cut(s) 87, 235, 244, 579, 830
PleI GAGTC 3 cut(s) 207, 516, 910
PpsI GAGTC 3 cut(s) 207, 516, 910
PscI ACATGT 1 cut(s) 901
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 315, 406
PspGI CCWGG 2 cut(s) 315, 406
PspN4I GGNNCC 1 cut(s) 670
PsuI RGATCY 1 cut(s) 614
RsaI GTAC 4 cut(s) 380, 536, 638, 717
RsaNI GTAC 4 cut(s) 379, 535, 637, 716
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 630
SatI GCNGC 5 cut(s) 86, 234, 243, 578, 829
Sau3AI GATC 5 cut(s) 15, 60, 220, 583, 614
SchI GAGTC 3 cut(s) 207, 516, 910
ScrFI CCNGG 5 cut(s) 317, 408, 437, 930, 931
SduI GDGCHC 2 cut(s) 257, 270
SetI ASST 8 cut(s) 90, 163, 270, 297, 553, 636, 703, 993
SfaNI GCATC 3 cut(s) 106, 198, 742
SfuI TTCGAA 1 cut(s) 202
SgrAI CRCCGGYG 1 cut(s) 665
SmaI CCCGGG 1 cut(s) 931
SmlI CTYRAG 3 cut(s) 310, 782, 992
SmoI CTYRAG 3 cut(s) 310, 782, 992
SpeI ACTAGT 1 cut(s) 857
Sse9I AATT 6 cut(s) 31, 123, 462, 810, 1045, 1051
SsiI CCGC 3 cut(s) 234, 459, 828
SspMI CTAG 2 cut(s) 431, 858
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 5 cut(s) 315, 406, 435, 928, 929
TaaI ACNGT 1 cut(s) 426
TaqI TCGA 7 cut(s) 202, 264, 510, 582, 681, 750, 966
TasI AATT 6 cut(s) 31, 123, 462, 810, 1045, 1051
TatI WGTACW 2 cut(s) 378, 715
TauI GCSGC 2 cut(s) 236, 831
TfiI GAWTC 3 cut(s) 372, 413, 895
Tru1I TTAA 1 cut(s) 630
Tru9I TTAA 1 cut(s) 630
TseI GCWGC 3 cut(s) 85, 242, 577
TspMI CCCGGG 1 cut(s) 929
XceI RCATGY 1 cut(s) 905
XmaI CCCGGG 1 cut(s) 929
XspI CTAG 2 cut(s) 431, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.