Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Forward (+)

21353497 .. 21363201

Gene Structure 9705 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G57660.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5013 bp

ATGGCTCACGCTCAAACCACAGAGGTGTGTTTGAGTTTTCACCGCTCTTTATTGTTTCCCATGGGAGCTTCCCAGGTGGTCGAATCTGTTAGATTCAGTTTCATGACAGAGCAAGATGTTCGAAAGCATAGTTTCCTGAAAGTTACCAGTCCTATTTTACACGATAACGTGGGCAATCCTTTTCCTGGTGGTCTCTATGATCTTAAATTAGGTCCTAAAGATGATAAGCAAGCTTGCAATTCATGTGGTCAGCTCAAACTTGCTTGTCCAGGACATTGTGGTCATATCGAGCTTGTCTTTCCTATTTACCATCCTTTGCTCTTCAATCTTCTTTTCAATTTCTTGCAGAGGGCCTGTTTCTTCTGTCATCACTTTATGGCAAAACCGGAAGATGTTGAAAGAGCTGTTTCTCAGCTGAAACTCATCATTAAAGGTGATATAGTTTCCGCGAAGCAATTGGAGTCCAACACTCCCACTAAGTCTAAGTCTAGTGATGAAAGCTGTGAATCAGTTGTAACCACTGATTCATCCGAAGAATGTGAAGATTCTGACGTGGAAGATCAGAGATGGACATCTCTTCAGTTTGCTGAAGTCACTGCTGTCTTGAAGAATTTTATGAGGTTGTCATCAAAGTCGTGTAGTAGATGCAAAGGAATTAATCCTAAACTCGAGAAGCCTATGTTCGGATGGGTTCGCATGAGAGCAATGAAAGACTCCGATGTTGGAGCTAATGTCATTCGAGGGCTTAAGCTAAAGAAGTCCACTAGCAGTGTTGAAAATCCAGATGGCTTTGATGATAGTGGCATTGATGCATTATCAGAAGTTGAAGATGGTGATAAAGAGACAAGAGAAAAGTCCACGGAAGTAGCTGCAGAGTTTGAAGAGCATAATTCTAAAAGAGACCTTTTACCGAGTGAGGTCAGGAATATCTTAAAACATCTGTGGCAGAATGAGCACGAGTTTTGCTCCTTCATTGGTGATCTTTGGCAGAGTGGGTCTGAAAAAATTGACTACTCTATGTTTTTTTTGGAGAGTGTTCTTGTGCCCCCTACTAAATTTAGACCTCCAACAACAGGAGGTGATAGTGTTATGGAACATCCTCAAACCGTAGGACTAAATAAGGTCATAGAATCCAATAATATACTCGGGAATGCCTGTACTAACAAATTAGATCAGTCGAAGGTAATTTTCAGATGGAGGAATCTTCAAGAATCAGTCAATGTCTTGTTTGATAGCAAAACTGCCACAGTTCAAAGCCAGAGAGACAGCTCTGGAATATGTCAATTGCTGGAGAAGAAAGAGGGGCTATTTCGGCAAAAAATGATGGGAAAGAGAGTGAATCATGCGTGCAGATCTGTGATATCTCCAGATCCTTATATCGCTGTTAATGACATCGGAATTCCTCCTTGTTTTGCTTTGAAATTGACGTATCCCGAGAGGGTGACTCCTTGGAATGTGGAGAAGTTAAGGGAAGCTATCATTAATGGTCCTGACATTCATCCTGGAGCTACACATTACTCTGACAAATCATCTACAATGAAGTTGCCCTCTACCGAAAAAGCACGAAGGGCAATTGCAAGAAAATTACTGTCCTCACGAGGAGCCACTACAGAACTTGGGAAAACTTGTGATATCAACTTTGAAGGTAAAACTGTCCATCGACATATGCGAGATGGTGACATCGTCCTTGTGAATCGTCAGCCAACATTACATAAACCTAGCTTAATGGCGCACAAGGTCCGTGTTCTGAAGGGGGAGAAAACACTTCGTCTCCACTATGCAAATTGCAGTACATACAATGCAGATTTTGATGGTGATGAGATGAATGTGCATTTTCCACAAGATGAGATTTCACGTGCTGAGGCCTACAATATTGTCAATGCTAACAACCAATATGCTAGGCCTTCTAATGGCGAGCCACTTCGAGCTTTAATTCAGGATCATATTGTCAGTTCCGTTTTGCTTACTAAAAGGGACACATTTTTGGACAAAGATCATTTTAATCAGCTTCTCTTCAGTTCCGGTGTCACTGACATGGTACTCAGTACTTTTTCTGGAAGATCTGGCAAGAAAGTCATGGTATCAGCTTCTGATGCAGAACTGTTGACAGTCACGCCTGCTATTTTGAAGCCAGTGCCTCTTTGGACTGGGAAGCAGGTCATCACTGCAGTGTTGAATCAGATAACCAAAGGTCACCCGCCTTTCACCGTAGAGAAAGCTACCAAACTTCCAGTTGATTTCTTCAAATGTAGATCTAGAGAAGTAAAACCTAACAGTGGAGATTTGACCAAAAAGAAAGAGATTGACGAGTCATGGAAACAGAATCTTAATGAAGATAAATTGCACATCAGAAAAAATGAATTCGTCTGCGGTGTGATCGACAAGGCTCAGTTTGCTGACTATGGGCTGGTTCATACAGTGCATGAGCTTTATGGTTCAAATGCTGCAGGAAATCTGCTCTCTGTTTTTAGTCGTCTCTTTACAGTCTTTCTTCAGACCCACGGTTTCACCTGTGGCGTTGATGATCTTATTATTTTAAAAGATATGGATGAAGAGAGGACGAAGCAACTTCAAGAATGTGAAAATGTTGGTGAAAGAGTACTCCGTAAAACCTTTGGCATAGATGTTGATGTTCAGATAGATCCTCAGGACATGAGATCCAGAATAGAACGAATTCTTTATGAGGACGGGGAATCTGCTTTGGCATCTTTGGACAGGAGCATCGTAAATTATCTCAACCAGTGTTCAAGCAAGGGTGTGATGAACGATTTACTGTCTGATGGGTTACTAAAAACACCTGGAAGGAATTGTATATCGCTAATGACGATATCTGGGGCTAAGGGCAGTAAGGTTAACTTCCAGCAAATCTCTTCACACCTTGGCCAGCAAGATTTAGAAGGAAAAAGAGTTCCCCGAATGGTTTCTGGGAAGACATTACCTTGTTTCCATCCATGGGACTGGTCCCCTAGAGCTGGGGGATTTATTAGTGACAGATTCCTAAGTGGTCTTCGCCCCCAAGAATATTACTTCCATTGCATGGCTGGACGAGAAGGGTTAGTTGATACTGCAGTGAAGACATCGCGTAGTGGGTATCTGCAAAGATGCTTGATGAAAAATCTTGAGAGTCTTAAAGTCAACTATGATTGTACTGTCCGGGATGCTGATGGGTCCATCATTCAGTTCCAATATGGTGAAGATGGTGTGGATGTTCATCGGTCTAGCTTCATTGAAAAGTTCAAAGAACTGACAATAAATCAAGATATGGTCCTTCAAAAATGCAGTGAAGACATGCTATCTGGAGCTAGTAGTTACATCAGTGATCTGCCTATTAGTCTTAAGAAGGGAGCTGAGAAGTTTGTAGAGGCAATGCCTATGAATGAACGTATTGCTTCTAAATTTGTGAGACAAGAAGAACTACTGAAGCTGGTGAAGTCAAAATTCTTTGCAAGTCTTGCGCAGCCAGGGGAGCCTGTAGGTGTACTTGCTGCTCAATCTGTAGGAGAGCCATCAACACAGATGACGTTGAATACTTTCCATCTTGCTGGACGTGGAGAGATGAATGTGACACTTGGAATTCCACGTTTACAAGAGATTTTGATGACAGCTGCAGCAAACATTAAGACACCAATAATGACATGCCCGTTGCTGAAAGGCAAAACAAAGGAGGATGCAAATGATATTACTGATAGGTTGAGGAAGATAACGGTGGCAGATATAATAAAGAGCATGGAACTGTCAGTTGTACCATATACGGTTTATGAGAATGAAGTTTGTAGTATTCACAAGCTTAAGATAAATCTCTATAAGCCAGAGCATTATCCGAAGCACACCGATATCACTGAAGAAGACTGGGAAGAGACGATGAGGGCTGTATTTTTAAGGAAGTTGGAGGACGCTATAGAGACCCACATGAAAATGCTCCATAGAATAAGGGGCATACACAATGATGTCACAGGTCCAATAGCTGGAAATGAAACTGATAATGATGATTCTGTAAGTGGAAAGCAGAATGAAGATGATGGTGATGATGATGGCGAGGGTACAGAAGTTGATGATCTTGGTTCAGATGCACAGAAACAGAAGAAACAAGAAACAGATGAGATGGATTATGAGGAAAACTCTGAAGATGAGACGAACGAACCATCTTCAATCAGTGGAGTAGAGGACCCTGAGATGGATAGTGAGAATGAGGACACAGAAGTTAGCAAGGAAGATACTCCGGAACCGCAGGAAGAGTCTATGGAACCTCAAAAAGAGGTAAAAGGGGTAAAGAATGTCAAAGAACAGAGTAAAAAAAAGAGACGGAAATTTGTTCGAGCTAAAAGTGACAGACATATCTTTGTGAAAGGTGAAGGAGAGAAATTTGAGGTCCATTTCAAGTTTGCTACAGATGACCCTCATATTTTACTTGCCCAGATTGCTCAACAAACGGCGCAGAAGGTTTACATCCAGAATTCTGGGAAGATTGAACGATGCACAGTGGCTAATTGTGGTGATCCTCAGGTGATCTACCATGGAGACAATCCAAAGGAGAGAAGAGAAATATCCAACGATGAAAAGAAAGCCTCACCAGCTCTCCACGCATCAGGAGTTGATTTCCCTGCACTGTGGGAATTCCAAGATAAACTTGACGTCAGGTACTTGTATAGCAACAGCATTCACGATATGCTCAACATTTTTGGAGTTGAAGCAGCTAGAGAAACAATAATCAGGGAAATAAACCACGTCTTCAAATCATATGGCATTTCTGTAAGCATCAGACACTTGAACCTCATTGCAGATTATATGACCTTTTCCGGTGGATACCGACCAATGAGCCGTATGGGAGGAATTGCAGAATCAACTTCACCATTCTGCAGAATGACTTTTGAAACAGCCACTAAGTTCATTGTCCAAGCAGCTACTTATGGAGAAAAGGACACATTAGAAACCCCCTCTGCTAGAATCTGTCTTGGCTTGCCTGCGCTTTCAGGAACTGGATGCTTCGATTTGATGCAGAGAGTGGAGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1670

Amino Acids

187.57

Weight (kDa)

5.93

Isoelectric Point (pI)

43.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	13 - 427	1.1e-36	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	430 - 614	5.1e-60	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	619 - 830	1.1e-33	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	884 - 982	3.2e-21	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	989 - 1600	3.2e-83	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	279
AatII	GACGTC	1 cut(s)	4635
Acc16I	TGCGCA	1 cut(s)	3456
Acc36I	ACCTGC	1 cut(s)	2146
AccB7I	CCANNNNNTGG	2 cut(s)	3037, 3965
AccBSI	CCGCTC	1 cut(s)	45
AccII	CGCG	2 cut(s)	449, 3082
AccIII	TCCGGA	1 cut(s)	4219
AciI	CCGC	5 cut(s)	43, 447, 2198, 2370, 4226
AclWI	GGATC	5 cut(s)	1364, 1947, 2636, 2652, 4490
AcoI	YGGCCR	1 cut(s)	2881
AcuI	CTGAAG	8 cut(s)	563, 609, 1769, 1999, 2477, 3440, 3861, 4143
AcvI	CACGTG	1 cut(s)	1856
AcyI	GRCGYC	1 cut(s)	4632
AflII	CTTAAG	3 cut(s)	746, 3335, 3788
AjiI	CACGTC	3 cut(s)	553, 3548, 4726
AjnI	CCWGG	6 cut(s)	72, 184, 268, 1501, 2797, 3460
AleI	CACNNNNGTG	2 cut(s)	23, 2168
AloI	GAACNNNNNNTCC	2 cut(s)	2892, 2924
Alw21I	GWGCWC	2 cut(s)	957, 5010
AlwI	GGATC	5 cut(s)	1364, 1947, 2636, 2652, 4490
AlwNI	CAGNNNCTG	2 cut(s)	2090, 4976
Ama87I	CYCGRG	3 cut(s)	668, 1145, 1433
Aor13HI	TCCGGA	1 cut(s)	4219
AoxI	GGCC	4 cut(s)	351, 1863, 1901, 2881
ApeKI	GCWGC	8 cut(s)	869, 2444, 3457, 3485, 3605, 3608, 4691, 4898
AseI	ATTAAT	2 cut(s)	657, 1482
Asp700I	GAANNNNTTC	3 cut(s)	2673, 2920, 3530
AspLEI	GCGC	4 cut(s)	1732, 3457, 4435, 4966
AsuC2I	CCSGG	1 cut(s)	3155
AsuII	TTCGAA	1 cut(s)	121
AvaI	CYCGRG	3 cut(s)	668, 1145, 1433
AvaII	GGWCC	9 cut(s)	212, 1487, 1738, 2961, 3168, 3265, 3956, 4165, 4369
AxyI	CCTNAGG	2 cut(s)	2646, 4500
BaeGI	GKGCMC	1 cut(s)	1047
BaeI	ACNNNNGTAYC	4 cut(s)	3054, 3087, 4032, 4065
BalI	TGGCCA	1 cut(s)	2883
BanII	GRGCYC	1 cut(s)	5010
BauI	CACGAG	2 cut(s)	956, 1596
BbrPI	CACGTG	1 cut(s)	1856
BbsI	GAAGAC	6 cut(s)	2936, 2999, 3080, 3291, 3852, 4720
Bbv12I	GWGCWC	2 cut(s)	957, 5010
BbvCI	CCTCAGC	1 cut(s)	1860
BbvI	GCAGC	8 cut(s)	856, 2431, 3469, 3472, 3592, 3620, 4703, 4910
BceAI	ACGGC	2 cut(s)	4446, 4803
BcgI	CGANNNNNNTGC	2 cut(s)	101, 135
BciT130I	CCWGG	6 cut(s)	74, 186, 270, 1503, 2799, 3462
BciVI	GTATCC	2 cut(s)	1440, 4796
BcnI	CCSGG	1 cut(s)	3155
BfrI	CTTAAG	3 cut(s)	746, 3335, 3788
BfuAI	ACCTGC	1 cut(s)	2146
BfuI	GTATCC	2 cut(s)	1440, 4796
BglII	AGATCT	3 cut(s)	1352, 2060, 2252
BisI	GCNGC	8 cut(s)	870, 2445, 3458, 3486, 3606, 3609, 4692, 4899
BlsI	GCNGC	8 cut(s)	871, 2446, 3459, 3487, 3607, 3610, 4693, 4900
BmcAI	AGTACT	2 cut(s)	2047, 2601
Bme1390I	CCNGG	7 cut(s)	74, 186, 270, 1503, 2799, 3155, 3462
Bme18I	GGWCC	9 cut(s)	212, 1487, 1738, 2961, 3168, 3265, 3956, 4165, 4369
BmeT110I	CYCGRG	3 cut(s)	668, 1145, 1433
BmgBI	CACGTC	3 cut(s)	553, 3548, 4726
BmiI	GGNNCC	7 cut(s)	1603, 2963, 3169, 3468, 4167, 4224, 4245
BmrFI	CCNGG	7 cut(s)	74, 186, 270, 1503, 2799, 3155, 3462
BmrI	ACTGGG	2 cut(s)	2157, 3859
BmuI	ACTGGG	2 cut(s)	2157, 3859
BpiI	GAAGAC	6 cut(s)	2936, 2999, 3080, 3291, 3852, 4720
BplI	GAGNNNNNCTC	7 cut(s)	950, 982, 1429, 1461, 4103, 4135, 4992
BpmI	CTGGAG	4 cut(s)	1310, 1350, 1524, 3318
Bpu10I	CCTNAGC	2 cut(s)	1860, 2838
Bpu14I	TTCGAA	1 cut(s)	121
BpuEI	CTTGAG	1 cut(s)	3140
BpuMI	CCSGG	1 cut(s)	3155
BsaAI	YACGTR	1 cut(s)	1856
BsaBI	GATNNNNATC	2 cut(s)	571, 3210
BsaHI	GRCGYC	1 cut(s)	4632
BsaI	GGTCTC	3 cut(s)	197, 894, 3896
BsaJI	CCNNGG	9 cut(s)	60, 72, 858, 1448, 2500, 2878, 2951, 3461, 4513
BsaWI	WCCGGW	4 cut(s)	385, 2021, 4219, 4796
BsaXI	ACNNNNNCTCC	6 cut(s)	717, 747, 1755, 1785, 4560, 4590
Bse1I	ACTGG	8 cut(s)	147, 2132, 2152, 2231, 2740, 2963, 3854, 4981
Bse21I	CCTNAGG	2 cut(s)	2646, 4500
Bse3DI	GCAATG	4 cut(s)	711, 3031, 3372, 4773
Bse8I	GATNNNNATC	2 cut(s)	571, 3210
BseAI	TCCGGA	1 cut(s)	4219
BseBI	CCWGG	6 cut(s)	74, 186, 270, 1503, 2799, 3462
BseDI	CCNNGG	9 cut(s)	60, 72, 858, 1448, 2500, 2878, 2951, 3461, 4513
BseJI	GATNNNNATC	2 cut(s)	571, 3210
BseMI	GCAATG	4 cut(s)	711, 3031, 3372, 4773
BseMII	CTCAG	8 cut(s)	425, 1851, 2056, 2402, 2660, 3339, 4161, 4514
BseNI	ACTGG	8 cut(s)	147, 2132, 2152, 2231, 2740, 2963, 3854, 4981
BseRI	GAGGAG	1 cut(s)	1614
BseSI	GKGCMC	1 cut(s)	1047
BseXI	GCAGC	8 cut(s)	856, 2431, 3469, 3472, 3592, 3620, 4703, 4910
BseYI	CCCAGC	1 cut(s)	2972
BsgI	GTGCAG	2 cut(s)	1369, 4587
Bsh1236I	CGCG	2 cut(s)	449, 3082
BshFI	GGCC	4 cut(s)	353, 1865, 1903, 2883
BsiHKAI	GWGCWC	2 cut(s)	957, 5010
BsiHKCI	CYCGRG	3 cut(s)	668, 1145, 1433
BsiSI	CCGG	5 cut(s)	386, 2022, 3154, 4220, 4797
BslFI	GGGAC	3 cut(s)	1988, 2947, 2969
BsmBI	CGTCTC	5 cut(s)	1775, 2480, 3851, 4124, 4294
BsmFI	GGGAC	3 cut(s)	1988, 2947, 2969
BsmI	GAATGC	2 cut(s)	1156, 4656
BsnI	GGCC	4 cut(s)	353, 1865, 1903, 2883
Bso31I	GGTCTC	3 cut(s)	197, 894, 3896
BsoBI	CYCGRG	3 cut(s)	668, 1145, 1433
Bsp119I	TTCGAA	1 cut(s)	121
Bsp1286I	GDGCHC	3 cut(s)	957, 1047, 5010
Bsp13I	TCCGGA	1 cut(s)	4219
Bsp19I	CCATGG	3 cut(s)	60, 2951, 4513
BspACI	CCGC	5 cut(s)	43, 447, 2198, 2370, 4226
BspANI	GGCC	4 cut(s)	353, 1865, 1903, 2883
BspCNI	CTCAG	8 cut(s)	424, 1852, 2055, 2401, 2659, 3340, 4162, 4513
BspEI	TCCGGA	1 cut(s)	4219
BspFNI	CGCG	2 cut(s)	449, 3082
BspHI	TCATGA	1 cut(s)	102
BspLI	GGNNCC	7 cut(s)	1603, 2963, 3169, 3468, 4167, 4224, 4245
BspMAI	CTGCAG	6 cut(s)	874, 2170, 2449, 3070, 3610, 4859
BspMI	ACCTGC	1 cut(s)	2146
BspPI	GGATC	5 cut(s)	1364, 1947, 2636, 2652, 4490
BspQI	GCTCTTC	2 cut(s)	326, 876
BspT104I	TTCGAA	1 cut(s)	121
BspTI	CTTAAG	3 cut(s)	746, 3335, 3788
BspTNI	GGTCTC	3 cut(s)	197, 894, 3896
BsrBI	CCGCTC	1 cut(s)	45
BsrDI	GCAATG	4 cut(s)	711, 3031, 3372, 4773
BsrI	ACTGG	8 cut(s)	147, 2132, 2152, 2231, 2740, 2963, 3854, 4981
BssECI	CCNNGG	9 cut(s)	60, 72, 858, 1448, 2500, 2878, 2951, 3461, 4513
BssNI	GRCGYC	1 cut(s)	4632
BssSI	CACGAG	2 cut(s)	956, 1596
BssT1I	CCWWGG	5 cut(s)	60, 1448, 2878, 2951, 4513
Bst2BI	CACGAG	2 cut(s)	956, 1596
Bst2UI	CCWGG	6 cut(s)	74, 186, 270, 1503, 2799, 3462
Bst6I	CTCTTC	9 cut(s)	326, 582, 876, 2018, 2547, 2875, 3849, 4227, 4531
BstACI	GRCGYC	1 cut(s)	4632
BstAFI	CTTAAG	3 cut(s)	746, 3335, 3788
BstAPI	GCANNNNNTGC	1 cut(s)	3452
BstBAI	YACGTR	1 cut(s)	1856
BstBI	TTCGAA	1 cut(s)	121
BstC8I	GCNNGC	8 cut(s)	231, 235, 1348, 1916, 2118, 2885, 4958, 4962
BstDSI	CCRYGG	5 cut(s)	60, 858, 2500, 2951, 4513
BstEII	GGTNACC	1 cut(s)	2192
BstFNI	CGCG	2 cut(s)	449, 3082
BstHHI	GCGC	4 cut(s)	1732, 3457, 4435, 4966
BstNI	CCWGG	6 cut(s)	74, 186, 270, 1503, 2799, 3462
BstNSI	RCATGY	2 cut(s)	3292, 3639
BstPI	GGTNACC	1 cut(s)	2192
BstSCI	CCNGG	7 cut(s)	72, 184, 268, 1501, 2797, 3153, 3460
BstSLI	GKGCMC	1 cut(s)	1047
BstUI	CGCG	2 cut(s)	449, 3082
BstV1I	GCAGC	8 cut(s)	856, 2431, 3469, 3472, 3592, 3620, 4703, 4910
BstV2I	GAAGAC	6 cut(s)	2936, 2999, 3080, 3291, 3852, 4720
BstX2I	RGATCY	6 cut(s)	1352, 1369, 2060, 2252, 2641, 2657
BstXI	CCANNNNNNTGG	3 cut(s)	2958, 3542, 4457
BstYI	RGATCY	6 cut(s)	1352, 1369, 2060, 2252, 2641, 2657
Bsu36I	CCTNAGG	2 cut(s)	2646, 4500
BsuI	GTATCC	2 cut(s)	1440, 4796
BsuRI	GGCC	4 cut(s)	353, 1865, 1903, 2883
BtgI	CCRYGG	5 cut(s)	60, 858, 2500, 2951, 4513
BtgZI	GCGATG	1 cut(s)	3063
BtrI	CACGTC	3 cut(s)	553, 3548, 4726
BtsI	GCAGTG	6 cut(s)	594, 775, 2163, 2175, 3075, 3286
BveI	ACCTGC	1 cut(s)	2146
Cac8I	GCNNGC	8 cut(s)	231, 235, 1348, 1916, 2118, 2885, 4958, 4962
CaiI	CAGNNNCTG	2 cut(s)	2090, 4976
CciI	TCATGA	1 cut(s)	102
CfoI	GCGC	4 cut(s)	1732, 3457, 4435, 4966
CseI	GACGC	1 cut(s)	3902
CspCI	CAANNNNNGTGG	2 cut(s)	226, 261
DraI	TTTAAA	1 cut(s)	2538
DrdI	GACNNNNNNGTC	1 cut(s)	279
DseDI	GACNNNNNNGTC	1 cut(s)	279
EaeI	YGGCCR	1 cut(s)	2881
Eam1104I	CTCTTC	9 cut(s)	326, 582, 876, 2018, 2547, 2875, 3849, 4227, 4531
EarI	CTCTTC	9 cut(s)	326, 582, 876, 2018, 2547, 2875, 3849, 4227, 4531
Ecl136II	GAGCTC	1 cut(s)	5008
Eco130I	CCWWGG	5 cut(s)	60, 1448, 2878, 2951, 4513
Eco147I	AGGCCT	2 cut(s)	1865, 1903
Eco24I	GRGCYC	1 cut(s)	5010
Eco31I	GGTCTC	3 cut(s)	197, 894, 3896
Eco32I	GATATC	4 cut(s)	1362, 1633, 2829, 3835
Eco47I	GGWCC	9 cut(s)	212, 1487, 1738, 2961, 3168, 3265, 3956, 4165, 4369
Eco53kI	GAGCTC	1 cut(s)	5008
Eco57I	CTGAAG	8 cut(s)	563, 609, 1769, 1999, 2477, 3440, 3861, 4143
Eco72I	CACGTG	1 cut(s)	1856
Eco81I	CCTNAGG	2 cut(s)	2646, 4500
Eco88I	CYCGRG	3 cut(s)	668, 1145, 1433
Eco91I	GGTNACC	1 cut(s)	2192
EcoICRI	GAGCTC	1 cut(s)	5008
EcoO109I	RGGNCCY	3 cut(s)	212, 351, 4165
EcoO65I	GGTNACC	1 cut(s)	2192
EcoRI	GAATTC	6 cut(s)	1398, 2360, 2673, 3573, 4453, 4613
EcoRII	CCWGG	6 cut(s)	72, 184, 268, 1501, 2797, 3460
EcoRV	GATATC	4 cut(s)	1362, 1633, 2829, 3835
EcoT14I	CCWWGG	5 cut(s)	60, 1448, 2878, 2951, 4513
EcoT22I	ATGCAT	1 cut(s)	814
EcoT38I	GRGCYC	1 cut(s)	5010
ErhI	CCWWGG	5 cut(s)	60, 1448, 2878, 2951, 4513
Esp3I	CGTCTC	5 cut(s)	1775, 2480, 3851, 4124, 4294
FalI	AAGNNNNNCTT	4 cut(s)	2840, 2872, 4611, 4643
FaqI	GGGAC	3 cut(s)	1988, 2947, 2969
FauI	CCCGC	1 cut(s)	2205
FauNDI	CATATG	2 cut(s)	1665, 4738
Fnu4HI	GCNGC	8 cut(s)	870, 2445, 3458, 3486, 3606, 3609, 4692, 4899
FriOI	GRGCYC	1 cut(s)	5010
Fsp4HI	GCNGC	8 cut(s)	870, 2445, 3458, 3486, 3606, 3609, 4692, 4899
FspI	TGCGCA	1 cut(s)	3456
GlaI	GCGC	4 cut(s)	1731, 3456, 4434, 4965
GluI	GCNGC	8 cut(s)	870, 2445, 3458, 3486, 3606, 3609, 4692, 4899
GsaI	CCCAGC	1 cut(s)	2976
GsuI	CTGGAG	4 cut(s)	1310, 1350, 1524, 3318
HaeIII	GGCC	4 cut(s)	353, 1865, 1903, 2883
HapII	CCGG	5 cut(s)	386, 2022, 3154, 4220, 4797
HgaI	GACGC	1 cut(s)	3902
HhaI	GCGC	4 cut(s)	1732, 3457, 4435, 4966
Hin1I	GRCGYC	1 cut(s)	4632
Hin6I	GCGC	4 cut(s)	1730, 3455, 4433, 4964
HinP1I	GCGC	4 cut(s)	1730, 3455, 4433, 4964
HincII	GTYRAC	3 cut(s)	2106, 2854, 3136
HindII	GTYRAC	3 cut(s)	2106, 2854, 3136
HindIII	AAGCTT	2 cut(s)	231, 3785
HpaI	GTTAAC	1 cut(s)	2854
HpaII	CCGG	5 cut(s)	386, 2022, 3154, 4220, 4797
Hpy166II	GTNNAC	8 cut(s)	762, 858, 2106, 2854, 3136, 3479, 3584, 4444
Hpy8I	GTNNAC	8 cut(s)	762, 858, 2106, 2854, 3136, 3479, 3584, 4444
Hsp92I	GRCGYC	1 cut(s)	4632
HspAI	GCGC	4 cut(s)	1730, 3455, 4433, 4964
Kpn2I	TCCGGA	1 cut(s)	4219
KspAI	GTTAAC	1 cut(s)	2854
LguI	GCTCTTC	2 cut(s)	326, 876
Lsp1109I	GCAGC	8 cut(s)	856, 2431, 3469, 3472, 3592, 3620, 4703, 4910
MbiI	CCGCTC	1 cut(s)	45
MfeI	CAATTG	3 cut(s)	455, 1283, 1572
MflI	RGATCY	6 cut(s)	1352, 1369, 2060, 2252, 2641, 2657
MhlI	GDGCHC	3 cut(s)	957, 1047, 5010
MlsI	TGGCCA	1 cut(s)	2883
MluNI	TGGCCA	1 cut(s)	2883
MlyI	GAGTC	6 cut(s)	470, 707, 1438, 2318, 3133, 4244
MmeI	TCCRAC	5 cut(s)	489, 703, 1091, 3867, 4572
Mox20I	TGGCCA	1 cut(s)	2883
Mph1103I	ATGCAT	1 cut(s)	814
MroI	TCCGGA	1 cut(s)	4219
MroXI	GAANNNNTTC	3 cut(s)	2673, 2920, 3530
MscI	TGGCCA	1 cut(s)	2883
MslI	CAYNNNNRTG	5 cut(s)	23, 2033, 2168, 3914, 3945
Msp20I	TGGCCA	1 cut(s)	2883
MspA1I	CMGCKG	2 cut(s)	415, 3605
MspCI	CTTAAG	3 cut(s)	746, 3335, 3788
MspI	CCGG	5 cut(s)	386, 2022, 3154, 4220, 4797
MspR9I	CCNGG	7 cut(s)	74, 186, 270, 1503, 2799, 3155, 3462
MunI	CAATTG	3 cut(s)	455, 1283, 1572
Mva1269I	GAATGC	2 cut(s)	1156, 4656
MvaI	CCWGG	6 cut(s)	74, 186, 270, 1503, 2799, 3462
MvnI	CGCG	2 cut(s)	449, 3082
NciI	CCSGG	1 cut(s)	3155
NcoI	CCATGG	3 cut(s)	60, 2951, 4513
NdeI	CATATG	2 cut(s)	1665, 4738
NlaIV	GGNNCC	7 cut(s)	1603, 2963, 3169, 3468, 4167, 4224, 4245
NsbI	TGCGCA	1 cut(s)	3456
NsiI	ATGCAT	1 cut(s)	814
NspI	RCATGY	2 cut(s)	3292, 3639
NspV	TTCGAA	1 cut(s)	121
OliI	CACNNNNGTG	2 cut(s)	23, 2168
PaeR7I	CTCGAG	1 cut(s)	668
PagI	TCATGA	1 cut(s)	102
PceI	AGGCCT	2 cut(s)	1865, 1903
PciSI	GCTCTTC	2 cut(s)	326, 876
PcsI	WCGNNNNNNNCGW	2 cut(s)	1666, 2822
PctI	GAATGC	2 cut(s)	1156, 4656
PdmI	GAANNNNTTC	3 cut(s)	2673, 2920, 3530
PflFI	GACNNNGTC	1 cut(s)	1682
PflMI	CCANNNNNTGG	2 cut(s)	3037, 3965
PfoI	TCCNGGA	3 cut(s)	268, 1501, 3153
PkrI	GCNGC	8 cut(s)	871, 2446, 3459, 3487, 3607, 3610, 4693, 4900
PleI	GAGTC	6 cut(s)	469, 707, 1438, 2317, 3132, 4243
PmaCI	CACGTG	1 cut(s)	1856
PmlI	CACGTG	1 cut(s)	1856
PpsI	GAGTC	6 cut(s)	469, 707, 1438, 2317, 3132, 4243
Ppu21I	YACGTR	1 cut(s)	1856
PpuMI	RGGWCCY	2 cut(s)	212, 4165
PshBI	ATTAAT	2 cut(s)	657, 1482
Psp124BI	GAGCTC	1 cut(s)	5010
Psp5II	RGGWCCY	2 cut(s)	212, 4165
Psp6I	CCWGG	6 cut(s)	72, 184, 268, 1501, 2797, 3460
PspCI	CACGTG	1 cut(s)	1856
PspEI	GGTNACC	1 cut(s)	2192
PspFI	CCCAGC	1 cut(s)	2972
PspGI	CCWGG	6 cut(s)	72, 184, 268, 1501, 2797, 3460
PspN4I	GGNNCC	7 cut(s)	1603, 2963, 3169, 3468, 4167, 4224, 4245
PspPPI	RGGWCCY	2 cut(s)	212, 4165
PstI	CTGCAG	6 cut(s)	874, 2170, 2449, 3070, 3610, 4859
PstNI	CAGNNNCTG	2 cut(s)	2090, 4976
PsuI	RGATCY	6 cut(s)	1352, 1369, 2060, 2252, 2641, 2657
PsyI	GACNNNGTC	1 cut(s)	1682
PvuII	CAGCTG	2 cut(s)	415, 3605
RseI	CAYNNNNRTG	5 cut(s)	23, 2033, 2168, 3914, 3945
SacI	GAGCTC	1 cut(s)	5010
SapI	GCTCTTC	2 cut(s)	326, 876
SatI	GCNGC	8 cut(s)	870, 2445, 3458, 3486, 3606, 3609, 4692, 4899
ScaI	AGTACT	2 cut(s)	2047, 2601
SchI	GAGTC	6 cut(s)	470, 707, 1438, 2318, 3133, 4244
ScrFI	CCNGG	7 cut(s)	74, 186, 270, 1503, 2799, 3155, 3462
SduI	GDGCHC	3 cut(s)	957, 1047, 5010
Sfr274I	CTCGAG	1 cut(s)	668
SfuI	TTCGAA	1 cut(s)	121
SinI	GGWCC	9 cut(s)	212, 1487, 1738, 2961, 3168, 3265, 3956, 4165, 4369
SlaI	CTCGAG	1 cut(s)	668
SmiMI	CAYNNNNRTG	5 cut(s)	23, 2033, 2168, 3914, 3945
SmlI	CTYRAG	5 cut(s)	668, 746, 3119, 3335, 3788
SmoI	CTYRAG	5 cut(s)	668, 746, 3119, 3335, 3788
SseBI	AGGCCT	2 cut(s)	1865, 1903
SsiI	CCGC	5 cut(s)	43, 447, 2198, 2370, 4226
SspI	AATATT	2 cut(s)	1873, 3023
SstI	GAGCTC	1 cut(s)	5010
StuI	AGGCCT	2 cut(s)	1865, 1903
StyD4I	CCNGG	7 cut(s)	72, 184, 268, 1501, 2797, 3153, 3460
StyI	CCWWGG	5 cut(s)	60, 1448, 2878, 2951, 4513
TaqII	GACCGA	1 cut(s)	3204
TatI	WGTACW	6 cut(s)	1157, 1790, 2045, 2599, 3146, 3478
TseI	GCWGC	8 cut(s)	869, 2444, 3457, 3485, 3605, 3608, 4691, 4898
TspGWI	ACGGA	5 cut(s)	875, 1730, 1945, 2594, 4318
Tth111I	GACNNNGTC	1 cut(s)	1682
Van91I	CCANNNNNTGG	2 cut(s)	3037, 3965
Vha464I	CTTAAG	3 cut(s)	746, 3335, 3788
VpaK11BI	GGWCC	9 cut(s)	212, 1487, 1738, 2961, 3168, 3265, 3956, 4165, 4369
VspI	ATTAAT	2 cut(s)	657, 1482
XbaI	TCTAGA	1 cut(s)	2255
XceI	RCATGY	2 cut(s)	3292, 3639
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2139, 3038
XhoI	CTCGAG	1 cut(s)	668
XmnI	GAANNNNTTC	3 cut(s)	2673, 2920, 3530
ZraI	GACGTC	1 cut(s)	4633
ZrmI	AGTACT	2 cut(s)	2047, 2601
Zsp2I	ATGCAT	1 cut(s)	814

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.