Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3B

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Reverse (-)

26881449 .. 26895890

Gene Structure 14442 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3BG275100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5037 bp

ATGGCCTCAGCTCAAACCTTAGAGGTTGCTACGGAGTCGGTGACGGCAGTTACGTTCAGCTTCTTTACAGATGAAGAGGCGCGAAATCAGAGCGTCGTGAAGGTTACGAAGCCGGATTTGGTTGACGAATTAGGAACGCCGGTGCAAGGTGGGCTTTACGATCCCGCAATGGGCCCTCTCGACGACAGAGGCAACGCACCTTGCAAAACATGTGGTCAGAGTGCGTTTAATTGCCGGGGTCATTTTGGCCATATTGACATCGATTTGCCCGCTTATAATCCCTTGTTGTTCAATGTTCTTTACAAACTTCTACAAAGAACCTGCTTATCCTGTCACCATTTTCGAACAAGCCGAGAGCTGGTTGAGAAATGTGTTTCCCAATTGGAACTCGTGATGAAAGGTGATGTTGTTGCGGCAAAAAGATTAGACTCTGACTCACCTGATGAATACTCATATCTTGGGGAGGACGGGGAAGGAAATGATATTGAGAGAGACAGGCATTCGAAGCCGGAAAGATGGACATCTCTTCAGTTCACAGAGGCAATGTCTATTTTGGATCGTTCTCTGGAGAAACAACAAAAGTGCAAGAATTGTAAATTTAAGAATCCTAAAATCACTAAACCGACTTTTGGTTGGTTTCAGATGAGTGGTGCTGGCAATGCTAGAGAAAATGTGATCAGAGGATGCAAGCTGGTAGGACCTTTAACTGTTCAAGAAGAGGGTGAATCTTGTGGTGACGATGTTGATGCAGGCATATCTGATATATCTTGTGCATCCTCTCTTGTGACTCAAGATACTGCAACGAAGAAGAGTAAAGGACAAGAGACTGTAGTACCCAGTGAGTTCTTTAAGCAGAAAAAAGCCTTCTCGGGAGATCTTTTACCAACCCAGGTTAAAGAAATAATGAGGCTTTTGTGGACAAATGAGGCTCAACTCTGCTCTTTTCTTAGTGATATCCAATGCCAAGGAGTTGAAAGAAAAGAAGGTCACTCTATGTTTTTCTTAGAGACTGTTCTTGTACCACCAATCAAATTTCGTCCTCCAGCCCATAGTGGTGATTCTGTGATGGAGCATCCTCAGACTGTTTCATTAAGTAAGGTGGTTCTGACAAATACTTACTTGAAAGACGCTCTTGGTAAGAACAAACACTCACAGACAATTCTGCGTTGGAGGCAACTGCAAGATTGTATAAATCTAATGTTCGATAGCAAAACTGTTGCGGGTCAAGGTAAATCAGATGCTTCTGGAATATGTCAGTTGCTGGAGAAAAAGGAAGGCATGTTCCGTCAGAAAATGATGGGAAAGAGGGTTAATAATGCGTGCCGGTCTGTTATATCCCCAGATCCCTATTTAGCTGTTAATGAGATTGGAATTCCACCGATCTTTGCATTGACATTAACATATCCTGAGAGAGTTACTCCGTGGAATGTTTTCAAGTTGAGGAATGCTATTGTCAATGGTTCTGAAATCCATCCTGGAGCTACGCACTATATTGATAAAACATCTATTAAGTTGCCACAAAAGCGGAGGGAGCGCTTGTTATTGTCAAGAAAGTTACCTTCTTCAAAAGGGGCAGGGACACAACATGGGAAGGGATCTGACTATGAAAACAAAATTGTACTCCGTCACTTGCAAGATGGGGACATTGTTCTTGTTAATCGCCAGCCTACACTTCACAAACCCAGTATAATGGCTCATATAGTTCGTGTATTGAAGGGGGAGAAAACATTACGCATGCACTATGCAAATTGCAGTACATACAATGCTGATTTTGATGGTGATGAAATGAATGTTCATTTCCCACAAGATGAAATTTCTCGCGCTGAAGCTTACAATATGGTGAACGCAAATAATCAATACGTAAAGCCCACTAGTGGTGATCCCATTCGAGCCTTGATTCAGGACCATGTTATAAGTGCTGTCCTCCTCACAAAGAAGGACACCTTTCTCCGCTCAGATGAATTTAATCAGCTTCTTTATAGTTCTTCTGTTTCAGCATGTGGTTCAGACTCTTTCTCTGGCAAGCCAGGTCAAAAGGTTCTAACGTCTAACTCTGAAGACGCAATGCAGCCCCTTCTTCCTGCAATTTGGAAACCAGTGCGTCTGTGGACTGGAAAGCAGGTCATCACCACGTTGCTAAACCATATTACAAGTGGTTCTCCACCACTTACTGTTGAAAAGGAAGCAAAACTCCCATCTGGGTTTTTCAACTGTAAGAATCTTGAAGAAGAAGAGAAATATGATAAAGGTCGACGAAAGAAAGAGAAGAAGCCAAGAAAAGAAGAGGCTGGTAAAGATGATTCAAAGGAAAAAGAGGATCCAGATGAGAGCAAGTTGTTAATCTATAGAAACAACTTGGTGCGTGGTGTGATCGACAAGAACCAGTTTGGTGATTATGGTTTGGTCCATAACGTGCAGGAACTCTATGGTTCGGAAGCTGCAGGAAAATTGCTTTCTGTTTTTAGTCGTTTATATACTGTTTATTTACAGATGCATGGGTTCACTTGTGGAGTTGACGATCTTTTGCTAATACAAAGCAAAGACAAAAAAATGAAGGAACAACTTGAAAGTTGTGAAGAGCTTGGTGAAAAAGTTTTCCGTGAGTTTATTGGGGTCAGACAAGATGAAAAAAAAGATCCGGTGATACTGCAGTCGGATATTGAAGAATTTTTACGCAGTAATGGAGAATCTGTGACAGAAATGTTGGACAGGAAAATGACAAGTGAACTTAATAAGAAAATCTCCAAATCTGATGTCTTCAAACAATTGTTTCGGAAAGGGTTATCAAAACCATCTGTTAAGAATTATATGTATCTTATGACAAGCTCTGGGGCTAAAGGAGGTGTGGCAAATCTCCAACAAATTGCTTCATACTTGGGTCAGCAAGAGCTAGAAGGGAAACGAGTCCCTCAAATGGTTTCTGGAAAGACCTTACCATGCTTCCCTCCATGGGATTGGTCACCTCGAGCTGGAGGTTTTATTATTGATCGCTTTCTTACCGGTCTTCATCCTCAGGAGTATTATTTTCACTGCATGGCTGGCCGTGAAGGGTTGGTTGATACAGCTGTCAAAACATCTCGTAGTGGATACTTGCAAAGATGCCTAATAAAAAATCTTGAATGCCTCAAGGTTTCTTATGACTATACTGTCCGTGATGCTGATGGTTCAATTATTCAATTCCGATATGGAGAGGATGGTATTGATGTCCATTTGACAAGCTTTATTAAAAAATTTGAAAAACTGGCAAATAATAAAGAAATGTTTGACAAGAAATTTGGTCGTCAAATTGACAAATTCAATCCCTATATTGATGAGTTGCCTCCCGCTCTCAAAAGAAAAGCAGAAATGTCAGTTCCTAATTTGTCTTTGAAGGAGAAAAATGCCAACTTGAAGTTAATGGAGCACAAATATCTCAGCAGTCTTGCGCAACCAGGAGAACCAGTTGGTATTCTTGCTGCTCAGTCTGTAGGAGAACCATCAACACAGATGACTTTGAATACCTTCCATAATGCTGGGCGAAGTGAAATGAATGTGACTCTTGGAATTCCACGGCTCCAAGAGATCTTGATGACTGCTTCAAATGATATCAAGACTCCAGTTATGACATGTCCATTGATAAAGGAGAAAGCGGGAGATGCTAAAAGTCTAGCTGATAGGTTGAAGAGGATCACTGTGGCAGACATAATAGAGAGCATGAAAGTAACTATTACACCATTTACAGATCAGTATCATCAGACTTGCCGTACTTATGAACTGGAGATGACACTTTTTATACCAGAATATCTTTCGAAATTTTCAGAAGATTGGGAGGAACTCCTAGAGGTTAAGTTTGTTAGGGCTTTGGAGGATGCCATACAAAATCATCTGGTTTTGCTTTCTAAAATTAGTGGTATAAAAGATGTCAAGGCTGATTCACTACCTAAGGCATCAAATGAGACAGATGAAGATGTTTCTTACAACATATCCCAGCATGGTGTGGAAGATGATGACGATGGCGGTGCAGATGACAGTGAATATGGTGAAGGAGCTGAGGATTTTGGGTTGGATGCACAAAAACGAAAGCGGCAGGGCACAGATGAGATGGAGTATGAAGATGACTGTGAGGATGAAGTGAATGAAGGTCATCAGTCCGATGGATTTGAAAGCGAAATTGATAAGGTGGAAAGTGATATTGAAAGTGATAAGGATGGTGTGACGCGGATTTCAGATGCTAATGATGAAATGCCTGAAAGTCCTTCAGCTGGAGACACATCTAAGCCACGGTCGAAGGGAAAGAAGACCAAATCAAGGGACAAAATTAAAAAGAAGACCAGAGCAAAGCTAGTAAGAAAGGAGTATGACAGGGCAACTTTTGTGTCAGTCAAGGGGTTCCACTTTGAGATCCATTTTAAATTTACTAATGAACCTCATATTCTTTTGGATCAGATTGCTCGGAAGACAGCTCGAAATGTTTATGTCAAAAGCTCTGGCAATATTTTGGATTGTAAAGATATTGATATTTATGGTAATGAAGATCAAGTTTTCTGCTCCTGGAATGATCCCAGAAATAAATTACCGGGTGAAAAGAATCCCATGGAAGAAGAAAAGAAGATGCGAGCCTTGCAGACAGCAGGGGTGGATTTTGCTACACTATGGAAACTGCAAGATGATCTTGATGTCAACTATATATATTCCAACAACATTCATGCAATGCTGAACACTTATGGAGTAGAAGCCGCCAGAGAAACTATCATCAAAGAAATAAGTAATGTCTTCAAGGTTTATGGGATATCTGTCAATATTAGACACCTTTCACTAATAGCTGACTACATGACTCATAGTGGCGGTTATCGTCCAATGAACAGATTTGGGGGTATAGCAGAGTCTATATCTCCGATCAATAAAATGTCATTTGAGACGGCTTCCAAGTTCATTCGTGAAGCAGCATATCATGGGGAGACAGATGACTTGGAAACCCCTTCTGCTAGGATCTGTCTTGGGCTGCCGGTGAAAGTGGGTACTGGTTGTTTTGATTTGATGCAAAAGATGGAGATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1678

Amino Acids

188.75

Weight (kDa)

6.19

Isoelectric Point (pI)

42.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	12 - 431	7e-31	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	434 - 615	4.4e-58	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	619 - 841	2.4e-29	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	894 - 994	6.3e-20	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	1001 - 1617	7.2e-84	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	276, 1913
AasI	GACNNNNNNGTC	1 cut(s)	3146
Acc16I	TGCGCA	1 cut(s)	3426
Acc36I	ACCTGC	2 cut(s)	329, 2110
AccBSI	CCGCTC	2 cut(s)	1953, 3326
AccI	GTMKAC	1 cut(s)	2251
AccII	CGCG	3 cut(s)	82, 1821, 4198
AcoI	YGGCCR	2 cut(s)	247, 3040
AcuI	CTGAAG	4 cut(s)	512, 1845, 2076, 4221
AfaI	GTAC	6 cut(s)	834, 1020, 1620, 1756, 3745, 4999
AfeI	AGCGCT	1 cut(s)	1535
AflIII	ACRYGT	2 cut(s)	209, 3605
AgeI	ACCGGT	1 cut(s)	2999
AhlI	ACTAGT	1 cut(s)	1871
AjnI	CCWGG	5 cut(s)	888, 1474, 2026, 3430, 4529
AjuI	GAANNNNNNNTTGG	2 cut(s)	101, 133
AloI	GAACNNNNNNTCC	2 cut(s)	1265, 1297
Alw21I	GWGCWC	1 cut(s)	3405
Alw26I	GTCTC	7 cut(s)	486, 818, 1001, 3929, 4239, 4889, 4931
AlwNI	CAGNNNCTG	1 cut(s)	1261
Ama87I	CYCGRG	2 cut(s)	868, 2964
Aor51HI	AGCGCT	1 cut(s)	1535
AoxI	GGCC	4 cut(s)	3, 172, 247, 3040
ApaI	GGGCCC	1 cut(s)	176
ApeKI	GCWGC	5 cut(s)	2068, 2438, 3455, 4922, 4981
ArsI	GACNNNNNNTTYG	4 cut(s)	2739, 2771, 3257, 3289
AsiGI	ACCGGT	1 cut(s)	2999
Asp700I	GAANNNNTTC	2 cut(s)	1430, 3500
AspLEI	GCGC	4 cut(s)	82, 1536, 1823, 3427
AspS9I	GGNCC	5 cut(s)	172, 173, 698, 1903, 2404
AsuC2I	CCSGG	2 cut(s)	236, 4557
AsuII	TTCGAA	3 cut(s)	343, 503, 3788
AvaI	CYCGRG	2 cut(s)	868, 2964
AvaII	GGWCC	3 cut(s)	698, 1903, 2404
AxyI	CCTNAGG	2 cut(s)	3012, 3921
BaeGI	GKGCMC	2 cut(s)	176, 4073
BaeI	ACNNNNGTAYC	6 cut(s)	816, 849, 2636, 2669, 3051, 3084
BalI	TGGCCA	1 cut(s)	249
BamHI	GGATCC	1 cut(s)	2317
BanII	GRGCYC	1 cut(s)	176
BarI	GAAGNNNNNNTAC	2 cut(s)	2655, 2687
BauI	CACGAG	1 cut(s)	389
BbsI	GAAGAC	7 cut(s)	2064, 2749, 2996, 4283, 4313, 4442, 4744
Bbv12I	GWGCWC	1 cut(s)	3405
BbvCI	CCTCAGC	2 cut(s)	7, 4029
BbvI	GCAGC	5 cut(s)	2080, 2425, 3442, 4934, 4968
BceAI	ACGGC	5 cut(s)	60, 3027, 3566, 3726, 4914
BcgI	CGANNNNNNTGC	4 cut(s)	728, 762, 3980, 4014
BciT130I	CCWGG	5 cut(s)	890, 1476, 2028, 3432, 4531
BciVI	GTATCC	1 cut(s)	3080
BclI	TGATCA	1 cut(s)	675
BcnI	CCSGG	2 cut(s)	236, 4557
BcoDI	GTCTC	7 cut(s)	486, 818, 1001, 3929, 4239, 4889, 4931
BcuI	ACTAGT	1 cut(s)	1871
BfaI	CTAG	7 cut(s)	663, 1872, 2891, 3647, 3818, 4322, 4965
BfmI	CTRYAG	5 cut(s)	828, 2344, 2439, 2648, 3465
BfoI	RGCGCY	1 cut(s)	1537
BfuAI	ACCTGC	2 cut(s)	329, 2110
BfuI	GTATCC	1 cut(s)	3080
BglII	AGATCT	2 cut(s)	874, 3561
BisI	GCNGC	8 cut(s)	414, 2069, 2439, 3456, 4064, 4716, 4923, 4982
BlsI	GCNGC	8 cut(s)	415, 2070, 2440, 3457, 4065, 4717, 4924, 4983
Bme1390I	CCNGG	7 cut(s)	236, 890, 1476, 2028, 3432, 4531, 4557
Bme18I	GGWCC	3 cut(s)	698, 1903, 2404
BmeT110I	CYCGRG	2 cut(s)	868, 2964
BmgT120I	GGNCC	5 cut(s)	172, 173, 698, 1903, 2404
BmiI	GGNNCC	4 cut(s)	174, 2319, 3554, 4370
BmrFI	CCNGG	7 cut(s)	236, 890, 1476, 2028, 3432, 4531, 4557
BmrI	ACTGGG	2 cut(s)	831, 1677
BmuI	ACTGGG	2 cut(s)	831, 1677
BpiI	GAAGAC	7 cut(s)	2064, 2749, 2996, 4283, 4313, 4442, 4744
BplI	GAGNNNNNCTC	2 cut(s)	1402, 1434
BpmI	CTGGAG	8 cut(s)	587, 1026, 1283, 1497, 2991, 3579, 3776, 4263
Bpu10I	CCTNAGC	2 cut(s)	7, 4029
Bpu14I	TTCGAA	3 cut(s)	343, 503, 3788
BpuEI	CTTGAG	2 cut(s)	774, 3110
BpuMI	CCSGG	2 cut(s)	236, 4557
Bsa29I	ATCGAT	1 cut(s)	261
BsaAI	YACGTR	1 cut(s)	1861
BsaBI	GATNNNNATC	2 cut(s)	520, 3726
BsaJI	CCNNGG	8 cut(s)	235, 888, 964, 1421, 2948, 3548, 4259, 4572
BsaWI	WCCGGW	2 cut(s)	2638, 2999
BsaXI	ACNNNNNCTCC	6 cut(s)	1523, 1553, 2676, 2706, 3426, 3456
Bse118I	RCCGGY	4 cut(s)	139, 1323, 2999, 4984
Bse21I	CCTNAGG	2 cut(s)	3012, 3921
Bse3DI	GCAATG	5 cut(s)	174, 549, 664, 2070, 4695
Bse8I	GATNNNNATC	2 cut(s)	520, 3726
BseBI	CCWGG	5 cut(s)	890, 1476, 2028, 3432, 4531
BseCI	ATCGAT	1 cut(s)	261
BseDI	CCNNGG	8 cut(s)	235, 888, 964, 1421, 2948, 3548, 4259, 4572
BseJI	GATNNNNATC	2 cut(s)	520, 3726
BseMI	GCAATG	5 cut(s)	174, 549, 664, 2070, 4695
BseMII	CTCAG	8 cut(s)	21, 1091, 1398, 1968, 3026, 3427, 3473, 4020
BseRI	GAGGAG	1 cut(s)	1916
BseSI	GKGCMC	2 cut(s)	176, 4073
BseXI	GCAGC	5 cut(s)	2080, 2425, 3442, 4934, 4968
BseYI	CCCAGC	2 cut(s)	3512, 3966
BsgI	GTGCAG	2 cut(s)	2435, 4020
Bsh1236I	CGCG	3 cut(s)	82, 1821, 4198
Bsh1285I	CGRYCG	1 cut(s)	4265
BshFI	GGCC	4 cut(s)	5, 174, 249, 3042
BshTI	ACCGGT	1 cut(s)	2999
BshVI	ATCGAT	1 cut(s)	261
BsiEI	CGRYCG	1 cut(s)	4265
BsiHKAI	GWGCWC	1 cut(s)	3405
BsiHKCI	CYCGRG	2 cut(s)	868, 2964
BsiSI	CCGG	9 cut(s)	113, 140, 235, 509, 1324, 2639, 3000, 4556, 4985
BslFI	GGGAC	4 cut(s)	1591, 1655, 2891, 4304
BsmAI	GTCTC	7 cut(s)	486, 818, 1001, 3929, 4239, 4889, 4931
BsmBI	CGTCTC	1 cut(s)	4889
BsmFI	GGGAC	4 cut(s)	1591, 1655, 2891, 4304
BsmI	GAATGC	3 cut(s)	499, 1450, 3125
BsnI	GGCC	4 cut(s)	5, 174, 249, 3042
BsoBI	CYCGRG	2 cut(s)	868, 2964
Bsp119I	TTCGAA	3 cut(s)	343, 503, 3788
Bsp120I	GGGCCC	1 cut(s)	172
Bsp1286I	GDGCHC	3 cut(s)	176, 3405, 4073
Bsp19I	CCATGG	2 cut(s)	2948, 4572
BspANI	GGCC	4 cut(s)	5, 174, 249, 3042
BspCNI	CTCAG	8 cut(s)	20, 1090, 1399, 1967, 3025, 3426, 3472, 4021
BspDI	ATCGAT	1 cut(s)	261
BspFNI	CGCG	3 cut(s)	82, 1821, 4198
BspLI	GGNNCC	4 cut(s)	174, 2319, 3554, 4370
BspMAI	CTGCAG	2 cut(s)	2443, 2652
BspMI	ACCTGC	2 cut(s)	329, 2110
BspQI	GCTCTTC	1 cut(s)	2571
BspT104I	TTCGAA	3 cut(s)	343, 503, 3788
BsrBI	CCGCTC	2 cut(s)	1953, 3326
BsrDI	GCAATG	5 cut(s)	174, 549, 664, 2070, 4695
BsrFI	RCCGGY	4 cut(s)	139, 1323, 2999, 4984
BssAI	RCCGGY	4 cut(s)	139, 1323, 2999, 4984
BssECI	CCNNGG	8 cut(s)	235, 888, 964, 1421, 2948, 3548, 4259, 4572
BssSI	CACGAG	1 cut(s)	389
BssT1I	CCWWGG	3 cut(s)	964, 2948, 4572
Bst2BI	CACGAG	1 cut(s)	389
Bst2UI	CCWGG	5 cut(s)	890, 1476, 2028, 3432, 4531
Bst6I	CTCTTC	8 cut(s)	69, 531, 711, 803, 2226, 2277, 2571, 3656
BstBAI	YACGTR	1 cut(s)	1861
BstBI	TTCGAA	3 cut(s)	343, 503, 3788
BstDSI	CCRYGG	5 cut(s)	1421, 2948, 3548, 4259, 4572
BstEII	GGTNACC	1 cut(s)	2958
BstFNI	CGCG	3 cut(s)	82, 1821, 4198
BstH2I	RGCGCY	1 cut(s)	1537
BstHHI	GCGC	4 cut(s)	82, 1536, 1823, 3427
BstMAI	GTCTC	7 cut(s)	486, 818, 1001, 3929, 4239, 4889, 4931
BstMCI	CGRYCG	1 cut(s)	4265
BstMWI	GCNNNNNNNGC	9 cut(s)	151, 505, 659, 1171, 1522, 1531, 3039, 3635, 4600
BstNI	CCWGG	5 cut(s)	890, 1476, 2028, 3432, 4531
BstNSI	RCATGY	5 cut(s)	213, 1282, 1738, 2001, 3609
BstPI	GGTNACC	1 cut(s)	2958
BstSCI	CCNGG	7 cut(s)	234, 888, 1474, 2026, 3430, 4529, 4555
BstSFI	CTRYAG	5 cut(s)	828, 2344, 2439, 2648, 3465
BstSLI	GKGCMC	2 cut(s)	176, 4073
BstSNI	TACGTA	1 cut(s)	1861
BstUI	CGCG	3 cut(s)	82, 1821, 4198
BstV1I	GCAGC	5 cut(s)	2080, 2425, 3442, 4934, 4968
BstV2I	GAAGAC	7 cut(s)	2064, 2749, 2996, 4283, 4313, 4442, 4744
BstX2I	RGATCY	8 cut(s)	874, 1342, 1595, 2317, 2635, 3561, 4380, 4968
BstXI	CCANNNNNNTGG	3 cut(s)	1690, 2955, 3512
BstYI	RGATCY	8 cut(s)	874, 1342, 1595, 2317, 2635, 3561, 4380, 4968
Bsu15I	ATCGAT	1 cut(s)	261
Bsu36I	CCTNAGG	2 cut(s)	3012, 3921
BsuI	GTATCC	1 cut(s)	3080
BsuRI	GGCC	4 cut(s)	5, 174, 249, 3042
BsuTUI	ATCGAT	1 cut(s)	261
BtgI	CCRYGG	5 cut(s)	1421, 2948, 3548, 4259, 4572
BtsI	GCAGTG	1 cut(s)	3028
BtsIMutI	CAGTG	5 cut(s)	844, 2103, 3028, 3669, 4015
BveI	ACCTGC	2 cut(s)	329, 2110
CaiI	CAGNNNCTG	1 cut(s)	1261
CfoI	GCGC	4 cut(s)	82, 1536, 1823, 3427
Cfr10I	RCCGGY	4 cut(s)	139, 1323, 2999, 4984
Cfr13I	GGNCC	5 cut(s)	172, 173, 698, 1903, 2404
ClaI	ATCGAT	1 cut(s)	261
CseI	GACGC	5 cut(s)	82, 1136, 2069, 2090, 4204
Csp6I	GTAC	6 cut(s)	833, 1019, 1619, 1755, 3744, 4998
CspAI	ACCGGT	1 cut(s)	2999
CspCI	CAANNNNNGTGG	2 cut(s)	193, 228
CviQI	GTAC	6 cut(s)	833, 1019, 1619, 1755, 3744, 4998
DraI	TTTAAA	1 cut(s)	4390
DrdI	GACNNNNNNGTC	1 cut(s)	3146
DseDI	GACNNNNNNGTC	1 cut(s)	3146
EaeI	YGGCCR	2 cut(s)	247, 3040
Eam1104I	CTCTTC	8 cut(s)	69, 531, 711, 803, 2226, 2277, 2571, 3656
EarI	CTCTTC	8 cut(s)	69, 531, 711, 803, 2226, 2277, 2571, 3656
Eco105I	TACGTA	1 cut(s)	1861
Eco130I	CCWWGG	3 cut(s)	964, 2948, 4572
Eco24I	GRGCYC	1 cut(s)	176
Eco32I	GATATC	3 cut(s)	955, 3586, 4770
Eco47I	GGWCC	3 cut(s)	698, 1903, 2404
Eco47III	AGCGCT	1 cut(s)	1535
Eco57I	CTGAAG	4 cut(s)	512, 1845, 2076, 4221
Eco81I	CCTNAGG	2 cut(s)	3012, 3921
Eco88I	CYCGRG	2 cut(s)	868, 2964
Eco91I	GGTNACC	1 cut(s)	2958
EcoO109I	RGGNCCY	2 cut(s)	173, 698
EcoO65I	GGTNACC	1 cut(s)	2958
EcoRI	GAATTC	2 cut(s)	1371, 3543
EcoRII	CCWGG	5 cut(s)	888, 1474, 2026, 3430, 4529
EcoRV	GATATC	3 cut(s)	955, 3586, 4770
EcoT14I	CCWWGG	3 cut(s)	964, 2948, 4572
EcoT22I	ATGCAT	1 cut(s)	2496
EcoT38I	GRGCYC	1 cut(s)	176
ErhI	CCWWGG	3 cut(s)	964, 2948, 4572
Esp3I	CGTCTC	1 cut(s)	4889
FalI	AAGNNNNNCTT	8 cut(s)	138, 170, 1116, 1148, 1928, 1960, 4635, 4667
FaqI	GGGAC	4 cut(s)	1591, 1655, 2891, 4304
FauI	CCCGC	5 cut(s)	172, 277, 1213, 3331, 3622
FbaI	TGATCA	1 cut(s)	675
FblI	GTMKAC	1 cut(s)	2251
Fnu4HI	GCNGC	8 cut(s)	414, 2069, 2439, 3456, 4064, 4716, 4923, 4982
FriOI	GRGCYC	1 cut(s)	176
Fsp4HI	GCNGC	8 cut(s)	414, 2069, 2439, 3456, 4064, 4716, 4923, 4982
FspBI	CTAG	7 cut(s)	663, 1872, 2891, 3647, 3818, 4322, 4965
FspI	TGCGCA	1 cut(s)	3426
GlaI	GCGC	4 cut(s)	81, 1535, 1822, 3426
GluI	GCNGC	8 cut(s)	414, 2069, 2439, 3456, 4064, 4716, 4923, 4982
GsaI	CCCAGC	2 cut(s)	3516, 3970
GsuI	CTGGAG	8 cut(s)	587, 1026, 1283, 1497, 2991, 3579, 3776, 4263
HaeII	RGCGCY	1 cut(s)	1537
HaeIII	GGCC	4 cut(s)	5, 174, 249, 3042
HapII	CCGG	9 cut(s)	113, 140, 235, 509, 1324, 2639, 3000, 4556, 4985
HgaI	GACGC	5 cut(s)	82, 1136, 2069, 2090, 4204
HhaI	GCGC	4 cut(s)	82, 1536, 1823, 3427
Hin6I	GCGC	4 cut(s)	80, 1534, 1821, 3425
HinP1I	GCGC	4 cut(s)	80, 1534, 1821, 3425
HincII	GTYRAC	4 cut(s)	124, 2252, 2515, 4660
HindII	GTYRAC	4 cut(s)	124, 2252, 2515, 4660
HindIII	AAGCTT	2 cut(s)	1827, 3217
HpaII	CCGG	9 cut(s)	113, 140, 235, 509, 1324, 2639, 3000, 4556, 4985
Hpy99I	CGWCG	3 cut(s)	98, 185, 2256
HpyCH4IV	ACGT	5 cut(s)	53, 1860, 2045, 2132, 2412
HpyF10VI	GCNNNNNNNGC	9 cut(s)	151, 505, 659, 1171, 1522, 1531, 3039, 3635, 4600
HpySE526I	ACGT	5 cut(s)	53, 1860, 2045, 2132, 2412
HspAI	GCGC	4 cut(s)	80, 1534, 1821, 3425
Ksp22I	TGATCA	1 cut(s)	675
LguI	GCTCTTC	1 cut(s)	2571
LmnI	GCTCC	7 cut(s)	1069, 1478, 1531, 3400, 3558, 4025, 4532
Lsp1109I	GCAGC	5 cut(s)	2080, 2425, 3442, 4934, 4968
MaeI	CTAG	7 cut(s)	663, 1872, 2891, 3647, 3818, 4322, 4965
MaeII	ACGT	5 cut(s)	53, 1860, 2045, 2132, 2412
MbiI	CCGCTC	2 cut(s)	1953, 3326
MfeI	CAATTG	2 cut(s)	380, 2765
MflI	RGATCY	8 cut(s)	874, 1342, 1595, 2317, 2635, 3561, 4380, 4968
MhlI	GDGCHC	3 cut(s)	176, 3405, 4073
MlsI	TGGCCA	1 cut(s)	249
MluNI	TGGCCA	1 cut(s)	249
MmeI	TCCRAC	6 cut(s)	1148, 2634, 2685, 2881, 4023, 4698
Mox20I	TGGCCA	1 cut(s)	249
Mph1103I	ATGCAT	1 cut(s)	2496
MroXI	GAANNNNTTC	2 cut(s)	1430, 3500
MscI	TGGCCA	1 cut(s)	249
MslI	CAYNNNNRTG	1 cut(s)	1053
Msp20I	TGGCCA	1 cut(s)	249
MspA1I	CMGCKG	2 cut(s)	3065, 4241
MspI	CCGG	9 cut(s)	113, 140, 235, 509, 1324, 2639, 3000, 4556, 4985
MspR9I	CCNGG	7 cut(s)	236, 890, 1476, 2028, 3432, 4531, 4557
MunI	CAATTG	2 cut(s)	380, 2765
Mva1269I	GAATGC	3 cut(s)	499, 1450, 3125
MvaI	CCWGG	5 cut(s)	890, 1476, 2028, 3432, 4531
MvnI	CGCG	3 cut(s)	82, 1821, 4198
MwoI	GCNNNNNNNGC	9 cut(s)	151, 505, 659, 1171, 1522, 1531, 3039, 3635, 4600
NciI	CCSGG	2 cut(s)	236, 4557
NcoI	CCATGG	2 cut(s)	2948, 4572
NlaIV	GGNNCC	4 cut(s)	174, 2319, 3554, 4370
NmeAIII	GCCGAG	1 cut(s)	377
NsbI	TGCGCA	1 cut(s)	3426
NsiI	ATGCAT	1 cut(s)	2496
NspI	RCATGY	5 cut(s)	213, 1282, 1738, 2001, 3609
NspV	TTCGAA	3 cut(s)	343, 503, 3788
PaeI	GCATGC	1 cut(s)	1738
PaeR7I	CTCGAG	1 cut(s)	2964
PciI	ACATGT	2 cut(s)	209, 3605
PciSI	GCTCTTC	1 cut(s)	2571
PcsI	WCGNNNNNNNCGW	2 cut(s)	50, 349
PctI	GAATGC	3 cut(s)	499, 1450, 3125
PdmI	GAANNNNTTC	2 cut(s)	1430, 3500
PfeI	GAWTC	9 cut(s)	604, 725, 1058, 1897, 2218, 2300, 2687, 3911, 4567
PfoI	TCCNGGA	2 cut(s)	1474, 4529
PinAI	ACCGGT	1 cut(s)	2999
PkrI	GCNGC	8 cut(s)	415, 2070, 2440, 3457, 4065, 4717, 4924, 4983
Ppu21I	YACGTR	1 cut(s)	1861
PpuMI	RGGWCCY	1 cut(s)	698
PscI	ACATGT	2 cut(s)	209, 3605
PsiI	TTATAA	2 cut(s)	276, 1913
Psp5II	RGGWCCY	1 cut(s)	698
Psp6I	CCWGG	5 cut(s)	888, 1474, 2026, 3430, 4529
PspEI	GGTNACC	1 cut(s)	2958
PspFI	CCCAGC	2 cut(s)	3512, 3966
PspGI	CCWGG	5 cut(s)	888, 1474, 2026, 3430, 4529
PspN4I	GGNNCC	4 cut(s)	174, 2319, 3554, 4370
PspOMI	GGGCCC	1 cut(s)	172
PspPI	GGNCC	5 cut(s)	172, 173, 698, 1903, 2404
PspPPI	RGGWCCY	1 cut(s)	698
PspXI	VCTCGAGB	1 cut(s)	2964
PstI	CTGCAG	2 cut(s)	2443, 2652
PstNI	CAGNNNCTG	1 cut(s)	1261
PsuI	RGATCY	8 cut(s)	874, 1342, 1595, 2317, 2635, 3561, 4380, 4968
PvuII	CAGCTG	2 cut(s)	3065, 4241
RsaI	GTAC	6 cut(s)	834, 1020, 1620, 1756, 3745, 4999
RsaNI	GTAC	6 cut(s)	833, 1019, 1619, 1755, 3744, 4998
RseI	CAYNNNNRTG	1 cut(s)	1053
SalI	GTCGAC	1 cut(s)	2250
SapI	GCTCTTC	1 cut(s)	2571
SatI	GCNGC	8 cut(s)	414, 2069, 2439, 3456, 4064, 4716, 4923, 4982
Sau96I	GGNCC	5 cut(s)	172, 173, 698, 1903, 2404
ScrFI	CCNGG	7 cut(s)	236, 890, 1476, 2028, 3432, 4531, 4557
SduI	GDGCHC	3 cut(s)	176, 3405, 4073
SfcI	CTRYAG	5 cut(s)	828, 2344, 2439, 2648, 3465
Sfr274I	CTCGAG	1 cut(s)	2964
SfuI	TTCGAA	3 cut(s)	343, 503, 3788
SgrAI	CRCCGGYG	1 cut(s)	139
SinI	GGWCC	3 cut(s)	698, 1903, 2404
SlaI	CTCGAG	1 cut(s)	2964
SmiMI	CAYNNNNRTG	1 cut(s)	1053
SmlI	CTYRAG	3 cut(s)	789, 2964, 3125
SmoI	CTYRAG	3 cut(s)	789, 2964, 3125
SnaBI	TACGTA	1 cut(s)	1861
SpeI	ACTAGT	1 cut(s)	1871
SphI	GCATGC	1 cut(s)	1738
SspI	AATATT	2 cut(s)	4474, 4780
SspMI	CTAG	7 cut(s)	663, 1872, 2891, 3647, 3818, 4322, 4965
StyD4I	CCNGG	7 cut(s)	234, 888, 1474, 2026, 3430, 4529, 4555
StyI	CCWWGG	3 cut(s)	964, 2948, 4572
TaiI	ACGT	5 cut(s)	56, 1863, 2048, 2135, 2415
TatI	WGTACW	2 cut(s)	1618, 1754
TauI	GCSGC	3 cut(s)	416, 4066, 4718
TfiI	GAWTC	9 cut(s)	604, 725, 1058, 1897, 2218, 2300, 2687, 3911, 4567
TscAI	CASTG	5 cut(s)	844, 2103, 3035, 3676, 4015
TseI	GCWGC	5 cut(s)	2068, 2438, 3455, 4922, 4981
TspGWI	ACGGA	6 cut(s)	47, 1274, 1410, 1613, 2588, 3140
TspRI	CASTG	5 cut(s)	844, 2103, 3035, 3676, 4015
VpaK11BI	GGWCC	3 cut(s)	698, 1903, 2404
XceI	RCATGY	5 cut(s)	213, 1282, 1738, 2001, 3609
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2103, 2150
XhoI	CTCGAG	1 cut(s)	2964
XmiI	GTMKAC	1 cut(s)	2251
XmnI	GAANNNNTTC	2 cut(s)	1430, 3500
XspI	CTAG	7 cut(s)	663, 1872, 2891, 3647, 3818, 4322, 4965
Zsp2I	ATGCAT	1 cut(s)	2496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.