Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Reverse (-)

7852110 .. 7871226

Gene Structure 19117 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1066600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5049 bp

ATGGCTTCCTCAGAGGTTCCGACGGCGTCCACTGAGGCGATTAAGTTTGCTTTCTTAACTGACGAGGAGGTGCGACAGCACAGCTTTCTGAGGGTTACCGACCCATCTCTCCTTACTCAATTAGGAAACCCCGTGGCCGGTGGGCTATATGATAAGGCACTCGGCCCAATTGATGACAAACCTGGCGAAAGTTGCGGAACATGTGGTGAGAAGTCGCGTAATTGCCCGGGTCATTGTGGTCATATTGACCTTGCGTTGCCAGTTTATAATCCCTTGCTGTTCAATACTCTTTTCAAACTTCTACAAAGAACGTGTTTCTTCTGCTACCATTTTCGGACAGACCAAACACATGTTGAGAAAGCTGTTTCCCAATTAAAACTCATAATGGAAGGTGACGTCGTTGGGGCTAAAATGTTGGATTCTGACTCACCTAGTGAATACTTAGACAACGAGGGAGATAGTCACAGTGACAAGCATGCAAAGCAGCAAGGATGGACTTCTCTTCAGTTTACGGAGCTAATGTCTGTTTTTGATAAGGCTTTGCCGAAACAAGGGAAGGACTGCAACAATTGCAAGGCGAAGAATCCCAAAATCATCAAACCTACTTTTGGATGGTTTCAAATGAGTGGCGCTGAAAATGCGAGAGAAAATGTTATCAGAGGGTGCAACCTGGTGGGACCCCTAACTGGTCAAGATGAAGGGGATGAGAATGAAAATGTTGACAATAATGATATGGAAAAGCACAATGGAGAAGGAAGCAAAGTGCCAAGTGAGTTCCTTAATCAGACAACTTCCTTGTCGGGAGATCTATTACCAAAACAGGTTCAAGACATCATAAAGCTTTTGTGGGAAAATGAAGCTGAGTTCTGCTCTTTTATTGGTGATATCCAATGCCATGGAATTGGAAGAAAAGCAGGTCACTCTATGTTTTTCTTAGAGACTGTACTTGTGCCACCAATCAAATTTCGTCCTCCTTCCAAGGGTGGTGATTCTGTGATGGAGCATCCTCAAACCGTTTTATTAAGTAAGGTGGTGCAGGCAAATAATTCTTTGCAAGAGTCTGTTGTTACCAAGTCCGACCCCTCAAAGACAATTCGGCAGTGGAGGGATCTTCAAGAGTACATAAATGTGATGTACAATAGCAAAACTGCTACTGGTAAAGGTCAAAAAGATGCAGATCCTGGAATATGTCAGTTGCTTGAGAAGAAGGAAGGCCTGTTCCGGCAGAAAATGATGGGCAAACGGGTTAATTATGCGTGTCGGTCTGTCATATCCCCAGATCCCTATTTAGCTGTTAATGAGATTGGAATTCCTCCGTATTTTGCAACGAGATTAACATATCCTGAGAGAGTTACTCCGTGGAATGTTGACAAGTTGAGGAATGCAATTGTCAACGGTCCTGAAATCCATCCTGGAGCTACACAGTATGTGGATAAGTCCTCTGTGAAGATGTTGAGACAAAACCGGAGAGAACGCATTTCAACAGCAAGAAAATTACCTTCTTCAAAAGGGCTTGCTACACAACACGGAAAAGGATCTGATAATAGCTATGAAAACAAAGTTGTTTTTCGTCACTTGCGAGATGGGGACATTGTGCTTGTCAATCGACAGCCTACACTTCACAAACCCAGTATAATGGCGCATGTAGTTCGTGTATTGAAGGGGGAGAAAACAATTCGCATGCACTATGCAAATTGCAGTACGTACAATGCTGATTTTGATGGTGATGAAATGAATGTTCATTTTCCACAAGATGAAATTTCTCGAGCAGAAGCTTACAATATCGTAAATGCAAATAACCAGTATGTAAAGCCTACCAGCGGTGATCCAATTAGAGCCTTGATTCAGGACCATGTCATAAGTGCTGTCCTCCTTACAAAGAAGGATACCTTTTTGCGCTGGGAGGAATTTAATCAGCTTCTTTATAGTTCATGTGTTTCAGCCCGTCGGTCAGATGCTTTGTCTGGAAAACCAGATCAAAAGGTTTTCATGTGTAACATTGAAGGCACAATGCAGCCCCTTCTTCCTGCTATTTGGAAACCAGAGCGTCTTTGGACTGGAAAACAGGTCATCACTGCCTTGCTAAATCATATTACTAGGGGTTCTCCGCCAGTTACTGTAGAAAAGGATGCAAAAATCCCACGTGGTTTTTTTAACTGTAATCTTGAAGAAGAGAAATTTGATGAAGAGGAACTACAGAAAAAGAAGAAACGAAGCAAAGGAAAACAAGCTGATGAATGTGGATCACAGGAGAAGAAACCAGACAAAAAAGTAGATAACGATAAATCAAAGGACACGGGGGTTGTAGATGAGAACAGTTTATTAATCTATAAAAACAATTTGGTGCGCGGTGTGATTGACAAGGCTCAGTTTGGTGATTATGGTTTGGTCCATACAGTGCAGGAGCTCTATGGCTCAGATGCTGCAGGAATATTGCTTTCTGTATTGAGTCGTTTATTTACTGTTTATTTGCAGATGCATGGGTTCACATGTGGTGTTGATGATCTTCTGGTGATGCCCAGCAAAGACACAAAAATGAAGGAACAACTTGAAAGTTGTGAAGAGATTGGTGAAAAAGTTTTCCGTGATTTTATTGAGGTCGAAGATGATAAAAGAAAAGATCTAGTGGCACTGCAATTGAACATTGAAAAATTTATACGGAGTAATGGAGAATCTGCCTTGGCAGCCTTGGATAGGAGAATGATTAGTCAGCTTAATAACAAGACCTCCAACTCTGACGTCTTCAAACAATTATTGTTGAAAGGGTTATCAAAACCGTCCGTTAAGAATTGTATCTATCTTATGACCACATCTGGGGCTAAAGGTAGTGTGGCCAACCTCCAACAAATAACTTCGTATTTGGGTCAGCAAGAGCTAGAAGGCAAACGGGTGCCTCAAATGGTTTCTGGAAAGACCTTACCGTGCTTTCACCCATGGGATTGGTCATCTCGATCTGGAGGCTTTATTATTGATCGTTTTCTTACCGGTCTTCGGCCTCAGGAGTATTATTTTCATTGTATGGCTGGTCGTGAAGGGTTGGTTGATACAGCTGTCAAAACATCTCGTAGTGGGTACCTGCAAAGGTGCCTAATAAAAAATCTTGAGTGCCTAAAAGTTTGTTATGACCATACTGTCCGTGATGCTGACGGTTCCATTGTTCAATTTTATTATGGAGAAGATGGTGTTGATGTCCATTTGACAAGCTTCCTACAAAAATTTGACTCCCTGGCAGCTAATAAAGAAATGTTCCAGAACAAATTCCATCGACAAATCGACAAATCTGATCCTTACATTTCTGAGCTGCCTTCAGCTCTCAAGTATAAAGCAGAGAAGTTCGTGCATGATTTGTCCTTGAAGGAGGAAAGTTATAACTTATTCAGCCAAGTGGATTTCTTGAAGTTAATGGAGCATAAATACCTCTCTAGTCTTGCTCAACCTGGAGAACCAGTTGGTGTTCTTGCTGCTCAGTCAGTAGGAGAACCTTCAACTCAGATGACACTGAATACTTTCCATCTTGCTGGAAGAGGTGAAATGAATGTGACACTTGGAATCCCTCGTCTTCAAGAGATCTTGATGACAGCTGCAAATGAGATTAAGACTCCGGTTATGACATGTCCATTGCGAAAAAGGAGATCAAAGGAGGAAGCTCAGCATCTAGCTAATAAGTTGAAGAAGATCACTGTGGCAGACATAATAGAGAGTATGGAAGTTACGTTGTTGCCATTTGTCCTTCAGGGTCTTGAAACTTGCCGTATTTATAAACTTCAAATGAAACTCCACATACCTGAACATCTTCAAAACATCATTCAAGATATATCAGAAGATTGGGAGGAAATCCTAGAGGTTATGTTTGTTAGGGCGTTGGAAGATGCCATACAAAGTCATGTGCTCTTGCTTTCCAAAATTAGTGGTATAAAAAATGTCACTGATGCACCACAAAAGGCATCAAATGAGATGGATGAAAATATTTCTAAAAGCGGACATCAACATCAGGAAGAAGACGATGACAATGATGGTGCAGACGATGGTGAAGGGGCCGAGGATTTTGGTATGGATGCACAAAAGCGAAAGCTGCAAGCTTTTGATGAGATGGATTATGAAGATGGATGTGAAGATGAAGTGAGAGATGGAGATCTGACAGATGGAGATGAAGTCGAAAATGATGCAGATAATGATGAGGAGAATGATGTTGAAACTGGTAAGGATGGTGCAATTGAGGTTGTAGATGCTACTGATGAAATGCCTGCAAATCCTTTAGAAGAGGCTGGAGACCTACTTAAGACCAAGTCAAAGGAGAAAAAGAACAAATCAGGAGCCACAATTAAAAAGAAAAAGATCAGAGCTCAGTTAGTAAGAAAAGATACCGACAGGTCAATATTTGTGTCAGCGAAGGGGTTCCACTTTGAGGTCCATTTTAAATTTACCGATGAACCTCATATTTTGCTGGCTCAGATTGCTCAGCAGACAGCCCAGAAGGTTTGTATGCAAAGATCTGGCAGTGTTGATGACTGTAAACAAATAACTTGTGACGAAAATCAAGTCCTCTTATACGGAAAGGATCCTGAGAATAAACAAAGTTATTCATCCAAGGAAAAAAAGGAGATGGCAGCTCTGCAGACCACTGGAGTGGATTTCCGTACATTTTGGAAATTGCAAGATGTTCTTGATGTCAGGTATTTATATTCCAACAACATACATGCCATGCTGAACACTTACGGAGTTGAAGCCGCCAGGGAAACTATCATCAGAGAAATTATGAACGTTTTCAAGTCTTACGGGATATCAGTCAACATTAGGCACCTTACGCTTATAGCTGACTACATGACTCACTCGGGTGGTTATCGACCGATGAATAGATTTGGAGGCATAGCAGAGTCGATATCTCCGTTTAATAAAATGTCTTTTGAGACAGCTTCTAAGTTCATTGTCGAAGCAGCATATCACGGGCAGACGGATGACTTGGAAACGCCATCCTCGAGGATCTGTCTTGGATTGCCAGTCAAGGTAGGCACTGGATGTTTTGATTTGATGCAGAAGATAGAGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1683

Amino Acids

189.07

Weight (kDa)

5.83

Isoelectric Point (pI)

43.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	12 - 410	2.7e-29	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	413 - 597	4.6e-60	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	601 - 836	7.5e-29	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	897 - 989	1.9e-20	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	996 - 1621	4.6e-85	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	267, 3369, 3759
AasI	GACNNNNNNGTC	1 cut(s)	3131
AatII	GACGTC	2 cut(s)	399, 2742
AbsI	CCTCGAGG	1 cut(s)	4975
Acc36I	ACCTGC	2 cut(s)	905, 3084
Acc65I	GGTACC	1 cut(s)	3072
AccB1I	GGYRCC	4 cut(s)	2890, 3072, 3084, 4797
AccII	CGCG	2 cut(s)	217, 2349
AciI	CCGC	6 cut(s)	195, 1821, 2108, 2349, 3978, 4728
AclI	AACGTT	1 cut(s)	4761
AcoI	YGGCCR	2 cut(s)	135, 2832
AcsI	RAATTY	9 cut(s)	962, 1308, 1758, 1907, 2177, 2651, 3215, 3257, 4418
AcuI	CTGAAG	3 cut(s)	488, 3291, 3716
AcvI	CACGTG	1 cut(s)	2144
AcyI	GRCGYC	3 cut(s)	26, 396, 2739
AdeI	CACNNNGTG	1 cut(s)	434
AfaI	GTAC	7 cut(s)	945, 1121, 1136, 1702, 1706, 3074, 4639
AflII	CTTAAG	1 cut(s)	4277
AflIII	ACRYGT	5 cut(s)	200, 311, 349, 2489, 3611
AgeI	ACCGGT	1 cut(s)	2984
AjnI	CCWGG	7 cut(s)	181, 669, 1180, 1411, 3225, 3436, 4730
AleI	CACNNNNGTG	2 cut(s)	4625, 4833
AloI	GAACNNNNNNTCC	2 cut(s)	1202, 1234
Alw21I	GWGCWC	3 cut(s)	2409, 3891, 4345
Alw26I	GTCTC	4 cut(s)	932, 1450, 4263, 4901
AlwNI	CAGNNNCTG	3 cut(s)	1181, 2117, 2423
Ama87I	CYCGRG	4 cut(s)	226, 1764, 4831, 4975
AoxI	GGCC	6 cut(s)	135, 163, 1213, 2832, 2993, 4035
ApoI	RAATTY	9 cut(s)	962, 1308, 1758, 1907, 2177, 2651, 3215, 3257, 4418
ArsI	GACNNNNNNTTYG	2 cut(s)	4524, 4556
AseI	ATTAAT	1 cut(s)	2324
AsiGI	ACCGGT	1 cut(s)	2984
Asp700I	GAANNNNTTC	5 cut(s)	3257, 3506, 3792, 4394, 4763
Asp718I	GGTACC	1 cut(s)	3072
AspLEI	GCGC	4 cut(s)	632, 1642, 1899, 2349
AspS9I	GGNCC	7 cut(s)	164, 677, 1397, 1849, 2389, 4035, 4408
AsuC2I	CCSGG	2 cut(s)	227, 228
AvaI	CYCGRG	4 cut(s)	226, 1764, 4831, 4975
AvaII	GGWCC	5 cut(s)	677, 1397, 1849, 2389, 4408
AxyI	CCTNAGG	1 cut(s)	2997
BaeI	ACNNNNGTAYC	2 cut(s)	3036, 3069
BalI	TGGCCA	1 cut(s)	2834
BamHI	GGATCC	1 cut(s)	4558
BanI	GGYRCC	4 cut(s)	2890, 3072, 3084, 4797
BanII	GRGCYC	2 cut(s)	2409, 4345
BarI	GAAGNNNNNNTAC	4 cut(s)	2178, 2210, 3858, 3890
BbrPI	CACGTG	1 cut(s)	2144
BbsI	GAAGAC	4 cut(s)	2734, 2981, 3551, 4005
Bbv12I	GWGCWC	3 cut(s)	2409, 3891, 4345
BceAI	ACGGC	2 cut(s)	39, 3735
BcgI	CGANNNNNNTGC	4 cut(s)	1631, 1665, 4145, 4179
BciT130I	CCWGG	7 cut(s)	183, 671, 1182, 1413, 3227, 3438, 4732
BciVI	GTATCC	1 cut(s)	1879
BcnI	CCSGG	2 cut(s)	227, 228
BcoDI	GTCTC	4 cut(s)	932, 1450, 4263, 4901
BfaI	CTAG	7 cut(s)	432, 2097, 2624, 2876, 3423, 3656, 3839
BfmI	CTRYAG	4 cut(s)	2118, 2195, 2424, 4613
BfoI	RGCGCY	1 cut(s)	633
BfrI	CTTAAG	1 cut(s)	4277
BfuAI	ACCTGC	2 cut(s)	905, 3084
BfuI	GTATCC	1 cut(s)	1879
BglII	AGATCT	5 cut(s)	805, 2620, 3567, 4132, 4490
BlpI	GCTNAGC	2 cut(s)	3648, 4458
Bme1390I	CCNGG	9 cut(s)	183, 227, 228, 671, 1182, 1413, 3227, 3438, 4732
Bme18I	GGWCC	5 cut(s)	677, 1397, 1849, 2389, 4408
BmeT110I	CYCGRG	4 cut(s)	226, 1764, 4831, 4975
BmgT120I	GGNCC	7 cut(s)	164, 677, 1397, 1849, 2389, 4035, 4408
BmrFI	CCNGG	9 cut(s)	183, 227, 228, 671, 1182, 1413, 3227, 3438, 4732
BmrI	ACTGGG	1 cut(s)	1623
BmuI	ACTGGG	1 cut(s)	1623
BpiI	GAAGAC	4 cut(s)	2734, 2981, 3551, 4005
BplI	GAGNNNNNCTC	4 cut(s)	854, 886, 1339, 1371
BpmI	CTGGAG	5 cut(s)	1434, 2976, 3459, 4287, 4644
Bpu1102I	GCTNAGC	2 cut(s)	3648, 4458
BpuEI	CTTGAG	3 cut(s)	1220, 3122, 3299
BpuMI	CCSGG	2 cut(s)	227, 228
BsaAI	YACGTR	2 cut(s)	1704, 2144
BsaBI	GATNNNNATC	2 cut(s)	1176, 4131
BsaHI	GRCGYC	3 cut(s)	26, 396, 2739
BsaI	GGTCTC	1 cut(s)	4263
BsaWI	WCCGGW	3 cut(s)	1464, 2984, 3601
BsaXI	ACNNNNNCTCC	8 cut(s)	506, 536, 2711, 2741, 3432, 3462, 4137, 4167
Bse118I	RCCGGY	2 cut(s)	137, 2984
Bse21I	CCTNAGG	1 cut(s)	2997
Bse3DI	GCAATG	1 cut(s)	3617
Bse8I	GATNNNNATC	2 cut(s)	1176, 4131
BseBI	CCWGG	7 cut(s)	183, 671, 1182, 1413, 3227, 3438, 4732
BseJI	GATNNNNATC	2 cut(s)	1176, 4131
BseMI	GCAATG	1 cut(s)	3617
BseRI	GAGGAG	2 cut(s)	80, 4193
BseYI	CCCAGC	2 cut(s)	1899, 2519
BsgI	GTGCAG	3 cut(s)	1055, 2420, 4038
Bsh1236I	CGCG	2 cut(s)	217, 2349
Bsh1285I	CGRYCG	1 cut(s)	4847
BshFI	GGCC	6 cut(s)	137, 165, 1215, 2834, 2995, 4037
BshNI	GGYRCC	4 cut(s)	2890, 3072, 3084, 4797
BshTI	ACCGGT	1 cut(s)	2984
BsiEI	CGRYCG	1 cut(s)	4847
BsiHKAI	GWGCWC	3 cut(s)	2409, 3891, 4345
BsiHKCI	CYCGRG	4 cut(s)	226, 1764, 4831, 4975
BsiSI	CCGG	6 cut(s)	138, 227, 1222, 1465, 2985, 3602
BslFI	GGGAC	2 cut(s)	690, 1601
BsmAI	GTCTC	4 cut(s)	932, 1450, 4263, 4901
BsmFI	GGGAC	2 cut(s)	690, 1601
BsmI	GAATGC	1 cut(s)	1387
BsnI	GGCC	6 cut(s)	137, 165, 1215, 2834, 2995, 4037
Bso31I	GGTCTC	1 cut(s)	4263
BsoBI	CYCGRG	4 cut(s)	226, 1764, 4831, 4975
Bsp1286I	GDGCHC	3 cut(s)	2409, 3891, 4345
Bsp1407I	TGTACA	1 cut(s)	1134
Bsp1720I	GCTNAGC	2 cut(s)	3648, 4458
Bsp19I	CCATGG	2 cut(s)	895, 2933
BspACI	CCGC	6 cut(s)	195, 1821, 2108, 2349, 3978, 4728
BspANI	GGCC	6 cut(s)	137, 165, 1215, 2834, 2995, 4037
BspFNI	CGCG	2 cut(s)	217, 2349
BspMAI	CTGCAG	2 cut(s)	2428, 4617
BspMI	ACCTGC	2 cut(s)	905, 3084
BspT107I	GGYRCC	4 cut(s)	2890, 3072, 3084, 4797
BspTI	CTTAAG	1 cut(s)	4277
BspTNI	GGTCTC	1 cut(s)	4263
BsrDI	GCAATG	1 cut(s)	3617
BsrFI	RCCGGY	2 cut(s)	137, 2984
BsrGI	TGTACA	1 cut(s)	1134
BssAI	RCCGGY	2 cut(s)	137, 2984
BssNI	GRCGYC	3 cut(s)	26, 396, 2739
BssT1I	CCWWGG	6 cut(s)	895, 978, 2679, 2688, 2933, 4587
Bst2UI	CCWGG	7 cut(s)	183, 671, 1182, 1413, 3227, 3438, 4732
Bst6I	CTCTTC	6 cut(s)	507, 2166, 2181, 2556, 3517, 4254
BstACI	GRCGYC	3 cut(s)	26, 396, 2739
BstAFI	CTTAAG	1 cut(s)	4277
BstAPI	GCANNNNNTGC	1 cut(s)	570
BstAUI	TGTACA	1 cut(s)	1134
BstBAI	YACGTR	2 cut(s)	1704, 2144
BstC8I	GCNNGC	7 cut(s)	477, 1038, 1515, 1682, 4077, 4245, 4446
BstDSI	CCRYGG	4 cut(s)	132, 895, 1358, 2933
BstEII	GGTNACC	1 cut(s)	94
BstENI	CCTNNNNNAGG	1 cut(s)	2692
BstFNI	CGCG	2 cut(s)	217, 2349
BstH2I	RGCGCY	1 cut(s)	633
BstHHI	GCGC	4 cut(s)	632, 1642, 1899, 2349
BstMAI	GTCTC	4 cut(s)	932, 1450, 4263, 4901
BstMCI	CGRYCG	1 cut(s)	4847
BstMWI	GCNNNNNNNGC	8 cut(s)	192, 481, 570, 638, 2684, 4072, 4454, 4804
BstNI	CCWGG	7 cut(s)	183, 671, 1182, 1413, 3227, 3438, 4732
BstNSI	RCATGY	8 cut(s)	204, 353, 479, 1646, 1684, 2493, 3615, 4700
BstPI	GGTNACC	1 cut(s)	94
BstSCI	CCNGG	9 cut(s)	181, 225, 226, 669, 1180, 1411, 3225, 3436, 4730
BstSFI	CTRYAG	4 cut(s)	2118, 2195, 2424, 4613
BstSNI	TACGTA	1 cut(s)	1704
BstUI	CGCG	2 cut(s)	217, 2349
BstV2I	GAAGAC	4 cut(s)	2734, 2981, 3551, 4005
BstXI	CCANNNNNNTGG	6 cut(s)	896, 902, 1636, 2940, 3518, 4627
Bsu36I	CCTNAGG	1 cut(s)	2997
BsuI	GTATCC	1 cut(s)	1879
BsuRI	GGCC	6 cut(s)	137, 165, 1215, 2834, 2995, 4037
BtgI	CCRYGG	4 cut(s)	132, 895, 1358, 2933
BtsI	GCAGTG	4 cut(s)	1106, 2073, 2630, 4504
BveI	ACCTGC	2 cut(s)	905, 3084
Cac8I	GCNNGC	7 cut(s)	477, 1038, 1515, 1682, 4077, 4245, 4446
CaiI	CAGNNNCTG	3 cut(s)	1181, 2117, 2423
CfoI	GCGC	4 cut(s)	632, 1642, 1899, 2349
Cfr10I	RCCGGY	2 cut(s)	137, 2984
Cfr13I	GGNCC	7 cut(s)	164, 677, 1397, 1849, 2389, 4035, 4408
Cfr9I	CCCGGG	1 cut(s)	226
CseI	GACGC	2 cut(s)	15, 2036
CsiI	ACCWGGT	1 cut(s)	669
Csp6I	GTAC	7 cut(s)	944, 1120, 1135, 1701, 1705, 3073, 4638
CspAI	ACCGGT	1 cut(s)	2984
CspCI	CAANNNNNGTGG	2 cut(s)	3889, 3924
CviQI	GTAC	7 cut(s)	944, 1120, 1135, 1701, 1705, 3073, 4638
DraI	TTTAAA	1 cut(s)	4417
DraIII	CACNNNGTG	1 cut(s)	434
DrdI	GACNNNNNNGTC	1 cut(s)	3131
DseDI	GACNNNNNNGTC	1 cut(s)	3131
EaeI	YGGCCR	2 cut(s)	135, 2832
Eam1104I	CTCTTC	6 cut(s)	507, 2166, 2181, 2556, 3517, 4254
EarI	CTCTTC	6 cut(s)	507, 2166, 2181, 2556, 3517, 4254
EciI	GGCGGA	1 cut(s)	2097
Ecl136II	GAGCTC	2 cut(s)	2407, 4343
Eco105I	TACGTA	1 cut(s)	1704
Eco130I	CCWWGG	6 cut(s)	895, 978, 2679, 2688, 2933, 4587
Eco147I	AGGCCT	1 cut(s)	1215
Eco24I	GRGCYC	2 cut(s)	2409, 4345
Eco31I	GGTCTC	1 cut(s)	4263
Eco32I	GATATC	3 cut(s)	886, 4782, 4881
Eco47I	GGWCC	5 cut(s)	677, 1397, 1849, 2389, 4408
Eco53kI	GAGCTC	2 cut(s)	2407, 4343
Eco57I	CTGAAG	3 cut(s)	488, 3291, 3716
Eco72I	CACGTG	1 cut(s)	2144
Eco81I	CCTNAGG	1 cut(s)	2997
Eco88I	CYCGRG	4 cut(s)	226, 1764, 4831, 4975
Eco91I	GGTNACC	1 cut(s)	94
EcoICRI	GAGCTC	2 cut(s)	2407, 4343
EcoNI	CCTNNNNNAGG	1 cut(s)	2692
EcoO109I	RGGNCCY	1 cut(s)	677
EcoO65I	GGTNACC	1 cut(s)	94
EcoRI	GAATTC	1 cut(s)	1308
EcoRII	CCWGG	7 cut(s)	181, 669, 1180, 1411, 3225, 3436, 4730
EcoRV	GATATC	3 cut(s)	886, 4782, 4881
EcoT14I	CCWWGG	6 cut(s)	895, 978, 2679, 2688, 2933, 4587
EcoT22I	ATGCAT	1 cut(s)	2481
EcoT38I	GRGCYC	2 cut(s)	2409, 4345
ErhI	CCWWGG	6 cut(s)	895, 978, 2679, 2688, 2933, 4587
FalI	AAGNNNNNCTT	4 cut(s)	1874, 1906, 4647, 4679
FaqI	GGGAC	2 cut(s)	690, 1601
FriOI	GRGCYC	2 cut(s)	2409, 4345
FspBI	CTAG	7 cut(s)	432, 2097, 2624, 2876, 3423, 3656, 3839
GlaI	GCGC	4 cut(s)	631, 1641, 1898, 2348
GsaI	CCCAGC	2 cut(s)	1903, 2523
GsuI	CTGGAG	5 cut(s)	1434, 2976, 3459, 4287, 4644
HaeII	RGCGCY	1 cut(s)	633
HaeIII	GGCC	6 cut(s)	137, 165, 1215, 2834, 2995, 4037
HapII	CCGG	6 cut(s)	138, 227, 1222, 1465, 2985, 3602
HgaI	GACGC	2 cut(s)	15, 2036
HhaI	GCGC	4 cut(s)	632, 1642, 1899, 2349
Hin1I	GRCGYC	3 cut(s)	26, 396, 2739
Hin6I	GCGC	4 cut(s)	630, 1640, 1897, 2347
HinP1I	GCGC	4 cut(s)	630, 1640, 1897, 2347
HincII	GTYRAC	4 cut(s)	721, 1369, 1393, 4789
HindII	GTYRAC	4 cut(s)	721, 1369, 1393, 4789
HindIII	AAGCTT	4 cut(s)	839, 1773, 3202, 4077
HpaII	CCGG	6 cut(s)	138, 227, 1222, 1465, 2985, 3602
Hpy166II	GTNNAC	8 cut(s)	30, 510, 721, 1369, 1393, 2487, 4514, 4789
Hpy8I	GTNNAC	8 cut(s)	30, 510, 721, 1369, 1393, 2487, 4514, 4789
Hpy99I	CGWCG	3 cut(s)	25, 401, 1950
HpyCH4IV	ACGT	7 cut(s)	311, 396, 1703, 2143, 2739, 3713, 4761
HpyF10VI	GCNNNNNNNGC	8 cut(s)	192, 481, 570, 638, 2684, 4072, 4454, 4804
HpySE526I	ACGT	7 cut(s)	311, 396, 1703, 2143, 2739, 3713, 4761
Hsp92I	GRCGYC	3 cut(s)	26, 396, 2739
HspAI	GCGC	4 cut(s)	630, 1640, 1897, 2347
KflI	GGGWCCC	1 cut(s)	677
KpnI	GGTACC	1 cut(s)	3076
LmnI	GCTCC	6 cut(s)	514, 1000, 1415, 2404, 3406, 4313
MabI	ACCWGGT	1 cut(s)	669
MaeI	CTAG	7 cut(s)	432, 2097, 2624, 2876, 3423, 3656, 3839
MaeII	ACGT	7 cut(s)	311, 396, 1703, 2143, 2739, 3713, 4761
MfeI	CAATTG	5 cut(s)	168, 568, 1386, 2636, 4212
MhlI	GDGCHC	3 cut(s)	2409, 3891, 4345
MlsI	TGGCCA	1 cut(s)	2834
MluNI	TGGCCA	1 cut(s)	2834
MlyI	GAGTC	7 cut(s)	419, 1067, 2458, 3215, 3592, 4819, 4883
MmeI	TCCRAC	7 cut(s)	44, 396, 1101, 2754, 2866, 3843, 4710
Mox20I	TGGCCA	1 cut(s)	2834
Mph1103I	ATGCAT	1 cut(s)	2481
MroXI	GAANNNNTTC	5 cut(s)	3257, 3506, 3792, 4394, 4763
MscI	TGGCCA	1 cut(s)	2834
MslI	CAYNNNNRTG	4 cut(s)	1127, 2534, 4625, 4833
Msp20I	TGGCCA	1 cut(s)	2834
MspA1I	CMGCKG	3 cut(s)	1821, 3050, 3581
MspCI	CTTAAG	1 cut(s)	4277
MspI	CCGG	6 cut(s)	138, 227, 1222, 1465, 2985, 3602
MspR9I	CCNGG	9 cut(s)	183, 227, 228, 671, 1182, 1413, 3227, 3438, 4732
MunI	CAATTG	5 cut(s)	168, 568, 1386, 2636, 4212
Mva1269I	GAATGC	1 cut(s)	1387
MvaI	CCWGG	7 cut(s)	183, 671, 1182, 1413, 3227, 3438, 4732
MvnI	CGCG	2 cut(s)	217, 2349
MwoI	GCNNNNNNNGC	8 cut(s)	192, 481, 570, 638, 2684, 4072, 4454, 4804
NciI	CCSGG	2 cut(s)	227, 228
NcoI	CCATGG	2 cut(s)	895, 2933
NmeAIII	GCCGAG	2 cut(s)	141, 4063
NmuCI	GTSAC	8 cut(s)	392, 461, 467, 917, 1571, 3538, 3922, 4526
NsiI	ATGCAT	1 cut(s)	2481
NspI	RCATGY	8 cut(s)	204, 353, 479, 1646, 1684, 2493, 3615, 4700
OliI	CACNNNNGTG	2 cut(s)	4625, 4833
PaeI	GCATGC	2 cut(s)	479, 1684
PaeR7I	CTCGAG	2 cut(s)	1764, 4975
PceI	AGGCCT	1 cut(s)	1215
PciI	ACATGT	4 cut(s)	200, 349, 2489, 3611
PcsI	WCGNNNNNNNCGW	3 cut(s)	1576, 4883, 4973
PctI	GAATGC	1 cut(s)	1387
PdmI	GAANNNNTTC	5 cut(s)	3257, 3506, 3792, 4394, 4763
PfeI	GAWTC	6 cut(s)	419, 583, 989, 1843, 2672, 3549
PflFI	GACNNNGTC	3 cut(s)	25, 1853, 4285
PfoI	TCCNGGA	2 cut(s)	1180, 1411
PinAI	ACCGGT	1 cut(s)	2984
PleI	GAGTC	7 cut(s)	419, 1066, 2457, 3215, 3592, 4819, 4882
PmaCI	CACGTG	1 cut(s)	2144
PmlI	CACGTG	1 cut(s)	2144
PpsI	GAGTC	7 cut(s)	419, 1066, 2457, 3215, 3592, 4819, 4882
Ppu21I	YACGTR	2 cut(s)	1704, 2144
PpuMI	RGGWCCY	1 cut(s)	677
PscI	ACATGT	4 cut(s)	200, 349, 2489, 3611
PshBI	ATTAAT	1 cut(s)	2324
PsiI	TTATAA	3 cut(s)	267, 3369, 3759
Psp124BI	GAGCTC	2 cut(s)	2409, 4345
Psp1406I	AACGTT	1 cut(s)	4761
Psp5II	RGGWCCY	1 cut(s)	677
Psp6I	CCWGG	7 cut(s)	181, 669, 1180, 1411, 3225, 3436, 4730
PspCI	CACGTG	1 cut(s)	2144
PspEI	GGTNACC	1 cut(s)	94
PspFI	CCCAGC	2 cut(s)	1899, 2519
PspGI	CCWGG	7 cut(s)	181, 669, 1180, 1411, 3225, 3436, 4730
PspPI	GGNCC	7 cut(s)	164, 677, 1397, 1849, 2389, 4035, 4408
PspPPI	RGGWCCY	1 cut(s)	677
PspXI	VCTCGAGB	1 cut(s)	4975
PstI	CTGCAG	2 cut(s)	2428, 4617
PstNI	CAGNNNCTG	3 cut(s)	1181, 2117, 2423
PsyI	GACNNNGTC	3 cut(s)	25, 1853, 4285
PvuII	CAGCTG	2 cut(s)	3050, 3581
RsaI	GTAC	7 cut(s)	945, 1121, 1136, 1702, 1706, 3074, 4639
RsaNI	GTAC	7 cut(s)	944, 1120, 1135, 1701, 1705, 3073, 4638
RseI	CAYNNNNRTG	4 cut(s)	1127, 2534, 4625, 4833
SacI	GAGCTC	2 cut(s)	2409, 4345
Sau96I	GGNCC	7 cut(s)	164, 677, 1397, 1849, 2389, 4035, 4408
SchI	GAGTC	7 cut(s)	419, 1067, 2458, 3215, 3592, 4819, 4883
ScrFI	CCNGG	9 cut(s)	183, 227, 228, 671, 1182, 1413, 3227, 3438, 4732
SduI	GDGCHC	3 cut(s)	2409, 3891, 4345
SexAI	ACCWGGT	1 cut(s)	669
SfcI	CTRYAG	4 cut(s)	2118, 2195, 2424, 4613
Sfr274I	CTCGAG	2 cut(s)	1764, 4975
SinI	GGWCC	5 cut(s)	677, 1397, 1849, 2389, 4408
SlaI	CTCGAG	2 cut(s)	1764, 4975
SmaI	CCCGGG	1 cut(s)	228
SmiMI	CAYNNNNRTG	4 cut(s)	1127, 2534, 4625, 4833
SmlI	CTYRAG	6 cut(s)	1199, 1764, 3101, 3314, 4277, 4975
SmoI	CTYRAG	6 cut(s)	1199, 1764, 3101, 3314, 4277, 4975
SnaBI	TACGTA	1 cut(s)	1704
SphI	GCATGC	2 cut(s)	479, 1684
SseBI	AGGCCT	1 cut(s)	1215
SsiI	CCGC	6 cut(s)	195, 1821, 2108, 2349, 3978, 4728
SspI	AATATT	3 cut(s)	2433, 3967, 4377
SspMI	CTAG	7 cut(s)	432, 2097, 2624, 2876, 3423, 3656, 3839
SstI	GAGCTC	2 cut(s)	2409, 4345
StuI	AGGCCT	1 cut(s)	1215
StyD4I	CCNGG	9 cut(s)	181, 225, 226, 669, 1180, 1411, 3225, 3436, 4730
StyI	CCWWGG	6 cut(s)	895, 978, 2679, 2688, 2933, 4587
TaiI	ACGT	7 cut(s)	314, 399, 1706, 2146, 2742, 3716, 4764
TaqII	GACCGA	3 cut(s)	1251, 1938, 4861
TatI	WGTACW	3 cut(s)	943, 1119, 1134
TauI	GCSGC	1 cut(s)	4730
TfiI	GAWTC	6 cut(s)	419, 583, 989, 1843, 2672, 3549
TseFI	GTSAC	8 cut(s)	392, 461, 467, 917, 1571, 3538, 3922, 4526
Tsp45I	GTSAC	8 cut(s)	392, 461, 467, 917, 1571, 3538, 3922, 4526
TspMI	CCCGGG	1 cut(s)	226
Tth111I	GACNNNGTC	3 cut(s)	25, 1853, 4285
Vha464I	CTTAAG	1 cut(s)	4277
VpaK11BI	GGWCC	5 cut(s)	677, 1397, 1849, 2389, 4408
VspI	ATTAAT	1 cut(s)	2324
XagI	CCTNNNNNAGG	1 cut(s)	2692
XapI	RAATTY	9 cut(s)	962, 1308, 1758, 1907, 2177, 2651, 3215, 3257, 4418
XceI	RCATGY	8 cut(s)	204, 353, 479, 1646, 1684, 2493, 3615, 4700
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2049
XhoI	CTCGAG	2 cut(s)	1764, 4975
XmaI	CCCGGG	1 cut(s)	226
XmnI	GAANNNNTTC	5 cut(s)	3257, 3506, 3792, 4394, 4763
XspI	CTAG	7 cut(s)	432, 2097, 2624, 2876, 3423, 3656, 3839
ZraI	GACGTC	2 cut(s)	397, 2740
Zsp2I	ATGCAT	1 cut(s)	2481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.