Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb5

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Reverse (-)

25601764 .. 25616308

Gene Structure 14545 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_5g35030.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5001 bp

ATGGCCTCAGCTCAAATCTTAGAGGTTCTGACGGAGTCGATAAGGGCGGTTAGTTTCAGCTTCTTTACAGACGAAGAGGCGTCAAAACAGAGTGTCATCAAGATTACTAAACCGGACTTGGTTGACGAATTAGGAACACCGGTGCAAGGTGGCCTTTATGATCCTGCAATGGGTCCTCTCGACGACAGAAGCCACACATGTTGCAGAACATGTGGCCAGAACGCGTATAATTGCCAAGGTCATTTTGGTCATATTGACATTGTTTTGCCGGTCTATAATCCATTGTTGTTCAATCATCTTTACAAACTTCTACAAAGAACCTGCTTCAATTGTTGCTGTTTTCGAACAAGCCGAGAGCAGGTTCAAAAATGTGTTTCCCAATTGGAACTCGTGATGAGAGGTGATGTTGTTGCGGCAAAGAGATTAGACTCTGACTCGCCTAATCATCACTCATATCTCGAGGAGGGCGGGGAAGGAAATGATACTGAGAGAGACAGGCATTCGAAGCCGGAAAGATGGACGTCTCTTCAGTTCACAGAAGCCATGTCTATTTTGAATTGTTCTTTGGAGACACAACAAACGTGCAAGAATTGTGGACACAAGAATCCTAAAATCACTAAACCGACTTTTGGTTGGTTTGAGAGGAGTGGTGCTGAAAATGCTAGAAAAAATGTAATCAGAAGATGCAAACTGGTAGGACCTTTAACTGTTCAAGAAGAGGGTGAATCTTGTGGCGACGATGTTGATGCAGGCATATCTGATATATCTTGTGCATCCTCATTTGTGACTCAAGTCACTGCAACGAAGAAGAGTGAAGAACAAGAGAGCATAGTGCTGGATCAGTATTTTAAGCAGAAAGAAGCCTTATCAGGAGATCTTACACCAAAAAAGGTAAAAGAATTAATGAGCCTTTTGTGGACAAAAGAGGCTCAACTCTGCTCTTATCTTAGTGATATCCAACACAATGGAGTGGAAAGAAAAGAAGGTCACTCTATGTTTTTCTTAGAGAGTGTTCTTGTACCACCTATCAAATTTCGTCCTCCGGCTCGTAGTGGTGATTCTGTGATGGAGCATGCGCAAACTGTTTCATTAGGTGAGGTGGTTCGGGCAAATTCATACTTGAAAGACGCTCTTGATAAGAACAAAGTGTCGCAGATAATTCAGTGCTGGAAGCGACTTCAAGATTGTATTAATCTAATGTTTGATGGTCAAGGTGGCATTCGTCAGATGCTGGAAAAGAAGGCAGGCATGTTTCGCCAGAAACTGATGGGAAAGAGGGTTAATTTTGCTTGCCGGTCTGTTATATCCCCAGATCCCTATTTAGCTGTTAATGAGATTGGAATTCCACCAATCTTTGCATTGACATTAACATATCCTGAGAGAGTTACTCCGTGGAATGTTTTCAAGTTGAGAAATGCTATTGTCAATGGTTCTGAAATCCATCCTGGAGCTACACACTATATTGATAAAACATCTATCAAGTTGCCGCAAAAGTGGAGGGAGCGCGTTTTATTGTCAAGAAAGTTACCTTCTTCGAAAGGGGCAGGGACACAACATGGGAAGGGATCTGACTACGAAAACAAAATTGTACTTCGTCACTTGCAAGATGGGGACATTGTTCTTGTTAATCGCCAGCCTACACTTCACAAACCCAGTATAATGGCTCATATAGTTCGTGTGTTGAAGGGGGAGAAAACATTACGCATGCACTATGCAAATTGCAGTACATACAATGCAGATTTTGATGGTGATGAAATGAATGTTCATTTTCCGCAAGATGAAATTTCTCGCGCTGAAGCTTACAATTTGGTGAACGCAAATAATCAATACGTAAAACCCACTAGTGGTTTTCCCATTCGAGCCTTAATTCAGGACCATATCATAAGTTCTCTGTTCCTTTCAAAAACGGACACATTCTTCGACTTGGATGAAATTAATCAGCTTCTGTATAGTTCTTCTGTTTCAGCATGTGGTTCAGAAACTTTCTCTGGCAAGCCAGGTCAAAAGGTTCTAATGTCCAACTCTGAAGATGCAATGCAGCTCCCCCTTCCTGCAATTTGGAAACCAGTGCGTAAGTGGCCTGGCAAGCAGATCATCACTACCTTGCTAAATCGAATTACAAGTGGTTCTCCCCCACTTACTTTTGAAAAGGAAGCAAAACTTCCATCTGGGTTTTTCAACTGTAAGACTCCTGTAGAAGAAGAGAGATCTGCTAAAGGTCACCGTGCAAAAGAGAAGCAGTCAAGAATAAAAGAAGCCGGTAAAGATGATTCAAGGGAAATAGAGGAGCCAGATGAGAGCAAGTTGTTAATCTACAAAAACAATTTGGTGCGTGGAGTGATTGACAAAAACCAGTTTGGTGATTATGGTTTGGTCCATAATGTGCACGAACTCTATGGTTCGGAAGCTGCAGGAAAATTGCTTTCTGCTTTTAGTCGTTTATACACTGTTTATTTACAGATGCACGGGTTCACATGTGGAGTTGACGATCTTTTGCTATTACAAAGCAAAGACAAAAAAATGAAGGAACAACTTGAAAGTTGTGAAGAGCTTGGTGAAAAAGTTATCCGTGAGTTTATTGGGGTCAGACAGAATGAAAGAAAAGGTGCATTGATATTGCAATCGGATATTGAAGAATTTTTGCGCAACAATGGAGAATCTGTGTCGGAAATGTTGGACAGGAAAATGACAAGTGAACTTAATAAGAAAATCTCCAATTCTGATGTCTTCAAACAATTGTTTCAGAAAGGTTTATCAAAACCATCTTTTAAGAATTGTATGTATCTTATGACAAGCTCTGGGGCTAAAGGTGGTGTGGCAAATCTCCAACAAATTGCTGCATACTTGGGTCAGCAAGAGCTAGAAGGCAAGCGAGTCCCTCAAATGGTTTCTGGAAAAACTTTACCATGCTTCCCTCCATGGGATTGGTCACCTCGAGCTGGAGGTTTTATTATTGATCGCTTTCTTACCGGTCTTCATCCTCAGGAGTATTATTTTCACTGCATGGCTGGCCGTGAAGGGCTGGTTGATACAGCTGTTAAAACTTCTCGTAGTGGATACTTGCAAAGATGCCTAATCAAGAATCTCGAATGCCTTAAGGTTTCTTATGACTATACTGTTCGTGATGCTGATGGTTCGATTATTCAATTCCGATACGGGGAGGATGGTATTGATGTCCATTTGACAAGTTTTATTCAAAAATTTGAAAAACTGGCGAATAATAAAGAAATGTTTGACAAGAGATTTGGTCGTGAAATTGATAAGTTCAATCCCTATATTGATGAGTTGCCTCCCGCTCTCAAAAGAAAAGCAGAGATGTCAGGTCCTGATTTGTCTTTGAAGGAAAAGAATGCCAACTTGAAGTTAATGGAGCACAAATTTCTCAGCAGTCTTGCGCAACCAGGAGAACCAGTTGGTATTCTTGCTGCTCAGTCTGTTGGAGAACCATCAACACAAATGACTCTGAATACCTTCCATAACGCTGGGCGAAGTGAAATGAATGTGACTCTTGGCATTCCACGGCTCCAAGAGATCTTGATGACTGCTTCAAATGATATCAAGACTCCAGTTATGACATGTCCATTGAGAATGGGGAAAGCGGAAGATGCTAAAAGTCTAGCTGGTAGGTTGAAGAGGATCACCGTGGCCGACATAATAGAGAGCATGAAAGTAAATATTAAACCATTTACACATCAGGACCATCAGACCTGCCGTGTTTATGAGCTTGAGATGACTTTTTTTATACCGGAACATCTTTCGAAATTTTCAGAAGATTGGGAGGAACTCCTAAAGGTCAAATTTGTCAGGGCTTTGGAGGATGCCATACAAAATCATCTTGTTTTGCTTTCTAAAATTAGTGGTATAAAAGATATAAAGGCTGATTCACTGCCGAAGGCATCAAATGAGACAGACGAAGATATTTCTTTTAACATATCCCAGCATGAGGGAGAAGGTGGTGACGATGACAATGTAGATGACAGTGGTGAAGGAGTTGAGGATTTTGGGTTGGATGCACAAAAACAAAAGCGACAGGGTACAGATGAGATGGAGTATGATGATGACTGGGAGGATGAATTTAATGAAGGTCGTCAATCTGATGAATTTGAAAACGAAATTGATAATGGGGAGCTTGATGTTGAGATTGATAAGGATGGTGTGACAGGGATCTCAGATGCTAATGATAAAATGCAAGAAAGTTGTTCAACTGGAGGTATATCTAAGCCACACTCGAAGGGAAAAAAGACCAAATCAAGAGATAAAATAAAAAAGAAGACCAGTGCAAAGCTAGTAAGAAAGGAGTATGACAGGGCAACTTTTGTGTCAGCCAAGGGCTTCCACTTTGAGATCCATTTTAAATTTACCAATGAACCTCATATTCTTTTGGATCAGATTGCTCAGAAGACAGCCCGAAATGTTTATGTCAAAAGCTCTGGCAATATTTTGGATTGTCAAGATATTGATATTTATGGTAATGAAGATCAAGTTTTCTGCTGCTGGAATGATCCCAGAAATAAATTATCGGGTGAAAAGAATCCCTCAGAAGAAGAAAAGAAGATGCGAGCCTTGCAGACAGCAGGCGTGGATTTTGCTACTCTATGGAAACTGCAAGATGATCTTGATGTCAACTATATATATTCCAACAACATTCATGCCATGCTGAACACTTACGGAGTAGAAGCCGCCAGAGAAACTATCATCAAAGAAATAAGTAACGTTTTCAAGGTGTATGGGATATCTGTCAATATTAGACACCTGTTACTGATAGCTGACTACATGACTCGTAGTGGTAGTTATCGTCCTATGAACAGATTTGGGGGAATAGCGGAGTCTACATCTCCGATGAATAAAATGTCATTTGAGACGGCTTCCAAGTTCATTCAGGAAGCGGCATATCATGGGGAGAAGGATGACTTGGAAACCCCATCTGCTAGGATCTGTCTTGGGCTGCCGGTGAAAGTGGGTACTGGATGTTTTGATTTGATGCACAAGATGGAGATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1667

Amino Acids

187.74

Weight (kDa)

6.19

Isoelectric Point (pI)

44.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	12 - 425	3.8e-31	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	424 - 605	1.4e-58	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	609 - 831	2.8e-28	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	884 - 984	2.5e-21	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	991 - 1609	9.4e-84	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	524
Acc16I	TGCGCA	3 cut(s)	1077, 2645, 3396
Acc36I	ACCTGC	3 cut(s)	329, 349, 3716
AccBSI	CCGCTC	1 cut(s)	3296
AccI	GTMKAC	1 cut(s)	4829
AccII	CGCG	3 cut(s)	224, 1506, 1791
AclI	AACGTT	1 cut(s)	4713
AcoI	YGGCCR	3 cut(s)	214, 3010, 3645
AcuI	CTGAAG	3 cut(s)	512, 1815, 2046
AcyI	GRCGYC	2 cut(s)	80, 521
AfaI	GTAC	5 cut(s)	1020, 1590, 1726, 4036, 4963
AflII	CTTAAG	1 cut(s)	3095
AflIII	ACRYGT	5 cut(s)	197, 209, 222, 2474, 3575
AgeI	ACCGGT	2 cut(s)	139, 2969
AhlI	ACTAGT	1 cut(s)	1841
AjnI	CCWGG	4 cut(s)	1444, 1996, 2080, 3400
AjuI	GAANNNNNNNTTGG	4 cut(s)	548, 580, 4895, 4927
Alw21I	GWGCWC	2 cut(s)	2388, 3375
Alw26I	GTCTC	5 cut(s)	486, 528, 563, 3899, 4853
Alw44I	GTGCAC	1 cut(s)	2384
AlwNI	CAGNNNCTG	4 cut(s)	1231, 1264, 1945, 3326
Ama87I	CYCGRG	2 cut(s)	458, 2934
AoxI	GGCC	6 cut(s)	3, 151, 214, 2078, 3010, 3645
ApaLI	GTGCAC	1 cut(s)	2384
ApeKI	GCWGC	6 cut(s)	2038, 2408, 2837, 3425, 4491, 4945
ArsI	GACNNNNNNTTYG	4 cut(s)	1901, 1933, 3227, 3259
AseI	ATTAAT	3 cut(s)	902, 1191, 1935
AsiGI	ACCGGT	2 cut(s)	139, 2969
Asp700I	GAANNNNTTC	2 cut(s)	1400, 3470
AspLEI	GCGC	5 cut(s)	1078, 1506, 1793, 2646, 3397
AspS9I	GGNCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
AsuII	TTCGAA	4 cut(s)	343, 503, 1535, 3758
AvaI	CYCGRG	2 cut(s)	458, 2934
AvaII	GGWCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
AxyI	CCTNAGG	1 cut(s)	2982
BaeGI	GKGCMC	1 cut(s)	2388
BaeI	ACNNNNGTAYC	2 cut(s)	3021, 3054
BalI	TGGCCA	1 cut(s)	216
BauI	CACGAG	1 cut(s)	389
BbsI	GAAGAC	4 cut(s)	2719, 2966, 4277, 4406
Bbv12I	GWGCWC	2 cut(s)	2388, 3375
BbvCI	CCTCAGC	1 cut(s)	7
BbvI	GCAGC	6 cut(s)	2050, 2395, 2824, 3412, 4478, 4932
BceAI	ACGGC	4 cut(s)	2997, 3536, 3696, 4878
BcgI	CGANNNNNNTGC	2 cut(s)	728, 762
BciT130I	CCWGG	4 cut(s)	1446, 1998, 2082, 3402
BciVI	GTATCC	1 cut(s)	3050
BcoDI	GTCTC	5 cut(s)	486, 528, 563, 3899, 4853
BcuI	ACTAGT	1 cut(s)	1841
BfaI	CTAG	6 cut(s)	663, 1842, 2861, 3617, 4286, 4929
BfmI	CTRYAG	2 cut(s)	2193, 2409
BfrI	CTTAAG	1 cut(s)	3095
BfuAI	ACCTGC	3 cut(s)	329, 349, 3716
BfuI	GTATCC	1 cut(s)	3050
BglII	AGATCT	3 cut(s)	874, 2207, 3531
Bme1390I	CCNGG	4 cut(s)	1446, 1998, 2082, 3402
Bme18I	GGWCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
BmeT110I	CYCGRG	2 cut(s)	458, 2934
BmgT120I	GGNCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
BmiI	GGNNCC	3 cut(s)	174, 2289, 3524
BmrFI	CCNGG	4 cut(s)	1446, 1998, 2082, 3402
BmrI	ACTGGG	2 cut(s)	1647, 4072
BmuI	ACTGGG	2 cut(s)	1647, 4072
BoxI	GACNNNNGTC	1 cut(s)	791
BpiI	GAAGAC	4 cut(s)	2719, 2966, 4277, 4406
BplI	GAGNNNNNCTC	2 cut(s)	1372, 1404
BpmI	CTGGAG	4 cut(s)	1467, 2961, 3549, 4227
Bpu10I	CCTNAGC	1 cut(s)	7
Bpu14I	TTCGAA	4 cut(s)	343, 503, 1535, 3758
BpuEI	CTTGAG	2 cut(s)	774, 3746
BsaAI	YACGTR	1 cut(s)	1831
BsaHI	GRCGYC	2 cut(s)	80, 521
BsaJI	CCNNGG	6 cut(s)	235, 1391, 2918, 3518, 3642, 4326
BsaWI	WCCGGW	4 cut(s)	112, 139, 2969, 3745
BsaXI	ACNNNNNCTCC	4 cut(s)	2646, 2676, 3396, 3426
Bse118I	RCCGGY	6 cut(s)	139, 268, 1293, 2258, 2969, 4948
Bse21I	CCTNAGG	1 cut(s)	2982
Bse3DI	GCAATG	2 cut(s)	174, 2040
BseBI	CCWGG	4 cut(s)	1446, 1998, 2082, 3402
BseDI	CCNNGG	6 cut(s)	235, 1391, 2918, 3518, 3642, 4326
BseMI	GCAATG	2 cut(s)	174, 2040
BseMII	CTCAG	9 cut(s)	21, 477, 1368, 2996, 3397, 3443, 4182, 4409, 4551
BseRI	GAGGAG	3 cut(s)	476, 658, 2300
BseSI	GKGCMC	1 cut(s)	2388
BseXI	GCAGC	6 cut(s)	2050, 2395, 2824, 3412, 4478, 4932
BseYI	CCCAGC	2 cut(s)	3482, 3936
Bsh1236I	CGCG	3 cut(s)	224, 1506, 1791
BshFI	GGCC	6 cut(s)	5, 153, 216, 2080, 3012, 3647
BshTI	ACCGGT	2 cut(s)	139, 2969
BsiHKAI	GWGCWC	2 cut(s)	2388, 3375
BsiHKCI	CYCGRG	2 cut(s)	458, 2934
BslFI	GGGAC	3 cut(s)	1561, 1625, 2861
BsmAI	GTCTC	5 cut(s)	486, 528, 563, 3899, 4853
BsmBI	CGTCTC	2 cut(s)	528, 4853
BsmFI	GGGAC	3 cut(s)	1561, 1625, 2861
BsmI	GAATGC	5 cut(s)	499, 1218, 3095, 3355, 3513
BsnI	GGCC	6 cut(s)	5, 153, 216, 2080, 3012, 3647
BsoBI	CYCGRG	2 cut(s)	458, 2934
Bsp119I	TTCGAA	4 cut(s)	343, 503, 1535, 3758
Bsp1286I	GDGCHC	2 cut(s)	2388, 3375
Bsp19I	CCATGG	1 cut(s)	2918
BspANI	GGCC	6 cut(s)	5, 153, 216, 2080, 3012, 3647
BspCNI	CTCAG	9 cut(s)	20, 478, 1369, 2995, 3396, 3442, 4181, 4408, 4550
BspFNI	CGCG	3 cut(s)	224, 1506, 1791
BspLI	GGNNCC	3 cut(s)	174, 2289, 3524
BspMAI	CTGCAG	1 cut(s)	2413
BspMI	ACCTGC	3 cut(s)	329, 349, 3716
BspQI	GCTCTTC	1 cut(s)	2541
BspT104I	TTCGAA	4 cut(s)	343, 503, 1535, 3758
BspTI	CTTAAG	1 cut(s)	3095
BsrBI	CCGCTC	1 cut(s)	3296
BsrDI	GCAATG	2 cut(s)	174, 2040
BsrFI	RCCGGY	6 cut(s)	139, 268, 1293, 2258, 2969, 4948
BssAI	RCCGGY	6 cut(s)	139, 268, 1293, 2258, 2969, 4948
BssECI	CCNNGG	6 cut(s)	235, 1391, 2918, 3518, 3642, 4326
BssNI	GRCGYC	2 cut(s)	80, 521
BssSI	CACGAG	1 cut(s)	389
BssT1I	CCWWGG	3 cut(s)	235, 2918, 4326
Bst2BI	CACGAG	1 cut(s)	389
Bst2UI	CCWGG	4 cut(s)	1446, 1998, 2082, 3402
Bst4CI	ACNGT	8 cut(s)	709, 1084, 2183, 2225, 2449, 3118, 3643, 3980
Bst6I	CTCTTC	7 cut(s)	69, 531, 711, 803, 2196, 2541, 3626
BstACI	GRCGYC	2 cut(s)	80, 521
BstAFI	CTTAAG	1 cut(s)	3095
BstBAI	YACGTR	1 cut(s)	1831
BstBI	TTCGAA	4 cut(s)	343, 503, 1535, 3758
BstDSI	CCRYGG	4 cut(s)	1391, 2918, 3518, 3642
BstEII	GGTNACC	2 cut(s)	2219, 2928
BstFNI	CGCG	3 cut(s)	224, 1506, 1791
BstHHI	GCGC	5 cut(s)	1078, 1506, 1793, 2646, 3397
BstMAI	GTCTC	5 cut(s)	486, 528, 563, 3899, 4853
BstMWI	GCNNNNNNNGC	8 cut(s)	505, 659, 1254, 2077, 2086, 3009, 3605, 4564
BstNI	CCWGG	4 cut(s)	1446, 1998, 2082, 3402
BstNSI	RCATGY	8 cut(s)	201, 213, 1076, 1252, 1708, 1971, 2478, 3579
BstPAI	GACNNNNGTC	1 cut(s)	791
BstPI	GGTNACC	2 cut(s)	2219, 2928
BstSCI	CCNGG	4 cut(s)	1444, 1996, 2080, 3400
BstSFI	CTRYAG	2 cut(s)	2193, 2409
BstSLI	GKGCMC	1 cut(s)	2388
BstSNI	TACGTA	1 cut(s)	1831
BstUI	CGCG	3 cut(s)	224, 1506, 1791
BstV1I	GCAGC	6 cut(s)	2050, 2395, 2824, 3412, 4478, 4932
BstV2I	GAAGAC	4 cut(s)	2719, 2966, 4277, 4406
BstX2I	RGATCY	8 cut(s)	874, 1312, 1565, 2207, 3531, 4164, 4344, 4932
BstXI	CCANNNNNNTGG	4 cut(s)	965, 1660, 2925, 3482
BstYI	RGATCY	8 cut(s)	874, 1312, 1565, 2207, 3531, 4164, 4344, 4932
Bsu36I	CCTNAGG	1 cut(s)	2982
BsuI	GTATCC	1 cut(s)	3050
BsuRI	GGCC	6 cut(s)	5, 153, 216, 2080, 3012, 3647
BtgI	CCRYGG	4 cut(s)	1391, 2918, 3518, 3642
BtsI	GCAGTG	3 cut(s)	795, 2998, 3884
BtsIMutI	CAGTG	8 cut(s)	795, 1169, 2073, 2445, 2998, 3884, 3985, 4282
BveI	ACCTGC	3 cut(s)	329, 349, 3716
CaiI	CAGNNNCTG	4 cut(s)	1231, 1264, 1945, 3326
CfoI	GCGC	5 cut(s)	1078, 1506, 1793, 2646, 3397
Cfr10I	RCCGGY	6 cut(s)	139, 268, 1293, 2258, 2969, 4948
Cfr13I	GGNCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
CseI	GACGC	2 cut(s)	69, 1136
Csp6I	GTAC	5 cut(s)	1019, 1589, 1725, 4035, 4962
CspAI	ACCGGT	2 cut(s)	139, 2969
CspCI	CAANNNNNGTGG	4 cut(s)	182, 217, 574, 609
CviQI	GTAC	5 cut(s)	1019, 1589, 1725, 4035, 4962
DraI	TTTAAA	1 cut(s)	4354
EaeI	YGGCCR	3 cut(s)	214, 3010, 3645
Eam1104I	CTCTTC	7 cut(s)	69, 531, 711, 803, 2196, 2541, 3626
EarI	CTCTTC	7 cut(s)	69, 531, 711, 803, 2196, 2541, 3626
Eco105I	TACGTA	1 cut(s)	1831
Eco130I	CCWWGG	3 cut(s)	235, 2918, 4326
Eco32I	GATATC	3 cut(s)	955, 3556, 4734
Eco47I	GGWCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
Eco57I	CTGAAG	3 cut(s)	512, 1815, 2046
Eco81I	CCTNAGG	1 cut(s)	2982
Eco88I	CYCGRG	2 cut(s)	458, 2934
Eco91I	GGTNACC	2 cut(s)	2219, 2928
EcoO109I	RGGNCCY	3 cut(s)	173, 698, 3323
EcoO65I	GGTNACC	2 cut(s)	2219, 2928
EcoRI	GAATTC	1 cut(s)	1341
EcoRII	CCWGG	4 cut(s)	1444, 1996, 2080, 3400
EcoRV	GATATC	3 cut(s)	955, 3556, 4734
EcoT14I	CCWWGG	3 cut(s)	235, 2918, 4326
ErhI	CCWWGG	3 cut(s)	235, 2918, 4326
Esp3I	CGTCTC	2 cut(s)	528, 4853
FaqI	GGGAC	3 cut(s)	1561, 1625, 2861
FauI	CCCGC	2 cut(s)	461, 3301
FblI	GTMKAC	1 cut(s)	4829
FspBI	CTAG	6 cut(s)	663, 1842, 2861, 3617, 4286, 4929
FspI	TGCGCA	3 cut(s)	1077, 2645, 3396
GlaI	GCGC	5 cut(s)	1077, 1505, 1792, 2645, 3396
GsaI	CCCAGC	2 cut(s)	3486, 3940
GsuI	CTGGAG	4 cut(s)	1467, 2961, 3549, 4227
HaeIII	GGCC	6 cut(s)	5, 153, 216, 2080, 3012, 3647
HgaI	GACGC	2 cut(s)	69, 1136
HhaI	GCGC	5 cut(s)	1078, 1506, 1793, 2646, 3397
Hin1I	GRCGYC	2 cut(s)	80, 521
Hin6I	GCGC	5 cut(s)	1076, 1504, 1791, 2644, 3395
HinP1I	GCGC	5 cut(s)	1076, 1504, 1791, 2644, 3395
HincII	GTYRAC	3 cut(s)	124, 2485, 4624
HindII	GTYRAC	3 cut(s)	124, 2485, 4624
HindIII	AAGCTT	1 cut(s)	1797
Hpy99I	CGWCG	2 cut(s)	185, 740
HpyCH4III	ACNGT	8 cut(s)	709, 1084, 2183, 2225, 2449, 3118, 3643, 3980
HpyCH4IV	ACGT	4 cut(s)	521, 581, 1830, 4713
HpyF10VI	GCNNNNNNNGC	8 cut(s)	505, 659, 1254, 2077, 2086, 3009, 3605, 4564
HpySE526I	ACGT	4 cut(s)	521, 581, 1830, 4713
Hsp92I	GRCGYC	2 cut(s)	80, 521
HspAI	GCGC	5 cut(s)	1076, 1504, 1791, 2644, 3395
LguI	GCTCTTC	1 cut(s)	2541
LmnI	GCTCC	8 cut(s)	1069, 1448, 1501, 2046, 2287, 3370, 3528, 4126
Lsp1109I	GCAGC	6 cut(s)	2050, 2395, 2824, 3412, 4478, 4932
MaeI	CTAG	6 cut(s)	663, 1842, 2861, 3617, 4286, 4929
MaeII	ACGT	4 cut(s)	521, 581, 1830, 4713
MbiI	CCGCTC	1 cut(s)	3296
MfeI	CAATTG	3 cut(s)	328, 380, 2735
MflI	RGATCY	8 cut(s)	874, 1312, 1565, 2207, 3531, 4164, 4344, 4932
MhlI	GDGCHC	2 cut(s)	2388, 3375
MlsI	TGGCCA	1 cut(s)	216
MluI	ACGCGT	1 cut(s)	222
MluNI	TGGCCA	1 cut(s)	216
MmeI	TCCRAC	8 cut(s)	982, 2043, 2646, 2655, 2851, 3418, 3987, 4662
Mox20I	TGGCCA	1 cut(s)	216
MroXI	GAANNNNTTC	2 cut(s)	1400, 3470
MscI	TGGCCA	1 cut(s)	216
Msp20I	TGGCCA	1 cut(s)	216
MspA1I	CMGCKG	1 cut(s)	3035
MspCI	CTTAAG	1 cut(s)	3095
MspR9I	CCNGG	4 cut(s)	1446, 1998, 2082, 3402
MunI	CAATTG	3 cut(s)	328, 380, 2735
Mva1269I	GAATGC	5 cut(s)	499, 1218, 3095, 3355, 3513
MvaI	CCWGG	4 cut(s)	1446, 1998, 2082, 3402
MvnI	CGCG	3 cut(s)	224, 1506, 1791
MwoI	GCNNNNNNNGC	8 cut(s)	505, 659, 1254, 2077, 2086, 3009, 3605, 4564
NcoI	CCATGG	1 cut(s)	2918
NlaIV	GGNNCC	3 cut(s)	174, 2289, 3524
NmeAIII	GCCGAG	1 cut(s)	377
NmuCI	GTSAC	9 cut(s)	784, 793, 986, 1595, 2219, 2928, 3502, 3956, 4156
NsbI	TGCGCA	3 cut(s)	1077, 2645, 3396
NspI	RCATGY	8 cut(s)	201, 213, 1076, 1252, 1708, 1971, 2478, 3579
NspV	TTCGAA	4 cut(s)	343, 503, 1535, 3758
PaeI	GCATGC	2 cut(s)	1076, 1708
PaeR7I	CTCGAG	2 cut(s)	458, 2934
PciI	ACATGT	4 cut(s)	197, 209, 2474, 3575
PciSI	GCTCTTC	1 cut(s)	2541
PcsI	WCGNNNNNNNCGW	1 cut(s)	349
PctI	GAATGC	5 cut(s)	499, 1218, 3095, 3355, 3513
PdmI	GAANNNNTTC	2 cut(s)	1400, 3470
PfeI	GAWTC	8 cut(s)	604, 725, 1058, 2270, 2657, 3082, 3881, 4531
PflFI	GACNNNGTC	1 cut(s)	34
PfoI	TCCNGGA	1 cut(s)	1444
PinAI	ACCGGT	2 cut(s)	139, 2969
Ppu21I	YACGTR	1 cut(s)	1831
PpuMI	RGGWCCY	3 cut(s)	173, 698, 3323
PscI	ACATGT	4 cut(s)	197, 209, 2474, 3575
PshAI	GACNNNNGTC	1 cut(s)	791
PshBI	ATTAAT	3 cut(s)	902, 1191, 1935
Psp1406I	AACGTT	1 cut(s)	4713
Psp5II	RGGWCCY	3 cut(s)	173, 698, 3323
Psp6I	CCWGG	4 cut(s)	1444, 1996, 2080, 3400
PspEI	GGTNACC	2 cut(s)	2219, 2928
PspFI	CCCAGC	2 cut(s)	3482, 3936
PspGI	CCWGG	4 cut(s)	1444, 1996, 2080, 3400
PspN4I	GGNNCC	3 cut(s)	174, 2289, 3524
PspPI	GGNCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
PspPPI	RGGWCCY	3 cut(s)	173, 698, 3323
PspXI	VCTCGAGB	1 cut(s)	2934
PstI	CTGCAG	1 cut(s)	2413
PstNI	CAGNNNCTG	4 cut(s)	1231, 1264, 1945, 3326
PsuI	RGATCY	8 cut(s)	874, 1312, 1565, 2207, 3531, 4164, 4344, 4932
PsyI	GACNNNGTC	1 cut(s)	34
PvuII	CAGCTG	1 cut(s)	3035
RsaI	GTAC	5 cut(s)	1020, 1590, 1726, 4036, 4963
RsaNI	GTAC	5 cut(s)	1019, 1589, 1725, 4035, 4962
SapI	GCTCTTC	1 cut(s)	2541
Sau96I	GGNCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
ScrFI	CCNGG	4 cut(s)	1446, 1998, 2082, 3402
SduI	GDGCHC	2 cut(s)	2388, 3375
SfcI	CTRYAG	2 cut(s)	2193, 2409
Sfr274I	CTCGAG	2 cut(s)	458, 2934
SfuI	TTCGAA	4 cut(s)	343, 503, 1535, 3758
SgrAI	CRCCGGYG	1 cut(s)	139
SinI	GGWCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
SlaI	CTCGAG	2 cut(s)	458, 2934
SmlI	CTYRAG	5 cut(s)	458, 789, 2934, 3095, 3725
SmoI	CTYRAG	5 cut(s)	458, 789, 2934, 3095, 3725
SnaBI	TACGTA	1 cut(s)	1831
SpeI	ACTAGT	1 cut(s)	1841
SphI	GCATGC	2 cut(s)	1076, 1708
SspI	AATATT	3 cut(s)	3676, 4438, 4744
SspMI	CTAG	6 cut(s)	663, 1842, 2861, 3617, 4286, 4929
StyD4I	CCNGG	4 cut(s)	1444, 1996, 2080, 3400
StyI	CCWWGG	3 cut(s)	235, 2918, 4326
TaaI	ACNGT	8 cut(s)	709, 1084, 2183, 2225, 2449, 3118, 3643, 3980
TaiI	ACGT	4 cut(s)	524, 584, 1833, 4716
TatI	WGTACW	2 cut(s)	1588, 1724
TauI	GCSGC	4 cut(s)	416, 1489, 4682, 4889
TfiI	GAWTC	8 cut(s)	604, 725, 1058, 2270, 2657, 3082, 3881, 4531
TscAI	CASTG	8 cut(s)	802, 1169, 2073, 2452, 3005, 3891, 3985, 4282
TseFI	GTSAC	9 cut(s)	784, 793, 986, 1595, 2219, 2928, 3502, 3956, 4156
TseI	GCWGC	6 cut(s)	2038, 2408, 2837, 3425, 4491, 4945
Tsp45I	GTSAC	9 cut(s)	784, 793, 986, 1595, 2219, 2928, 3502, 3956, 4156
TspGWI	ACGGA	5 cut(s)	47, 1380, 1922, 2558, 4683
TspRI	CASTG	8 cut(s)	802, 1169, 2073, 2452, 3005, 3891, 3985, 4282
Tth111I	GACNNNGTC	1 cut(s)	34
Vha464I	CTTAAG	1 cut(s)	3095
VneI	GTGCAC	1 cut(s)	2384
VpaK11BI	GGWCC	6 cut(s)	173, 698, 1873, 2374, 3323, 3697
VspI	ATTAAT	3 cut(s)	902, 1191, 1935
XceI	RCATGY	8 cut(s)	201, 213, 1076, 1252, 1708, 1971, 2478, 3579
XcmI	CCANNNNNNNNNTGG	2 cut(s)	242, 2073
XhoI	CTCGAG	2 cut(s)	458, 2934
XmiI	GTMKAC	1 cut(s)	4829
XmnI	GAANNNNTTC	2 cut(s)	1400, 3470
XspI	CTAG	6 cut(s)	663, 1842, 2861, 3617, 4286, 4929
ZraI	GACGTC	1 cut(s)	522

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.