Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Forward (+)

22871601 .. 22885804

Gene Structure 14204 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00400730.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4698 bp

ATGAAAGGTGATGTTGTTGCGGCAAAAAGATTAGACTCTGACTCACCTGATGAATACTCATATCTTGGGGAGGACGGGGAAGGAAATGATATTGAGAGAGACAGGCATTCGAAGCCGGAAAGATGGACATCTCTTCAGTTCACAGAGGCAATGTCTATTTTGGATCGTTCTCTGGAGAAACAACAAAAGTGCAAGAATTGTAAATTTAAGAATCCTAAAATCACTAAACCGACTTTTGGTTGGTTTCAGATGAGTGGTGCTGGCAATGCTAGAGAAAATGTAATCAGAGGATGCAAGCTGGTAGGACCTTTAACTGTTCAAGAAGAGGGTGAATCTTGTGGTGATGATGTTGATGCAGGCATATCTGATATATCTTGTGCATCCTCTCTTGTGACTCAAGATACTGCAACGAAGAAGAGTAAAGGACAAGAGACCGTAGTGCCCAGTGAGTTCTTTAAGCAGAAAAAAGCCTTCTCGGGAGATCTTTTACCAACCCAGGTTAAAGAAATAATGAGGCTTTTGTGGACAAATGAGGCTCAACTCTGCTCTTTTCTTAGTGATATCCAATGCCAAGGAGTTGAAAGAAAAGAAGGTCACTCTATGTTTTTCCTAGATACTGTTCTTGTACCACCAATCAAATTTCGTCCTCCAGCCCATAGTGGTGATTCTGTGATGGAGCATCCTCAGACTGTTTCATTAAGTAAGGTGGTTCTGACAAATACTTACTTGAAAGACGCTCTTGGTAAGAACAAACACTCACAGACAATTCTGCGTTGGAGGCAACTGCAAGATTGTATAAATCTAATGTTCGATAGCAAAACTGTTGCGGGTCAAGGTAAATCAGATGCTTCTGGAATTTGTCAGTTGCTGGAGAAAAAGGAAGGCATGTTCCGTCAGAAAATGATGGGAAAGAGGGTTAATAATGCGTGCCGGTCTGTTATATCCCCAGATCCCTATTTAGCTGTTAATGAGATTGGAATTCCTCCGATCTTTGCATTGACATTAACATATCCTGAGAGAGTTACTCCATGGAATGTTTTCAAGTTGAGGAATGCTATAGTCAATGGTTCTGAAATCCATCCTGGAGCTACGCACTATATTGATAAAACATCTATTAAGTTGCCTCAAAAGCGGAGGGAGCGCTTGTTATTGTCAAGAAAGTTACCTTCTTCAAAAGGGGCAGGGACACAACATGGGAAGGGATCTGACTATGAAAACAAAATTGTACTCCGTCACTTGCAAGATGGGGACATTGTTCTTGTTAATCGCCAGCCTACACTTCACAAACCCAGTATAATGGCTCATATTGTTCGTGTATTGAAGGGGGAGAAAACATTACGCATGCACTATGCAAATTGCAGTACATACAATGCTGATTTTGATGGTGATGAAATGAATGTTCATTTCCCACAAGATGAAATTTCTCGCGCTGAAGCTTACAATATGGTGAACGCAAATAATCAATACGTAAAACCCACTAGTGGTGATCCCATTCGAGCCTTGATTCAGGACCATGTTATAAGCGCTGTCCTCCTCACAAAAAAGGACACCTTTCTCCGCTCAGATGAATTTAATCAGCTTCTTTATAGTTCTTCTGTTTCAGCATGTGGTTCAGACTCTTTCTCTGGCAAGCCAGGTCAAAAGGTTCTAACGTCTAACTCTGAAGACACACTGCAGCCCCTTCTTCCTGCAATTTGGAAACCAGTGCGTCTGTGGACTGGAAAGCAGGTCATCACTACGTTGCTAAACCATATTACAAGTGGTTCTCCACCACTTACTGTTGAAAAGGAAGCAAAACTCCCATCTGGGTTTTTTAACTGTAAGAATCTTGAAGAAGAAGAGAAATCTGATGAAGGTCGACGAAAGAAAGAGAAGAAGCCAAGAAAAGAAGAGGCTGGTAAAGATGATTCAAAGGAAAAAGAGGATCCAGATGAGAGCAAGCTGTTAATCTATAGAAACAACTTGGTCCGTGGTGTGATTGACAAGAACCAGTTTGGTGATTATGGTTTGGTCCATAACGTGCAGGAACTCTATGGTTCGGAAGCTGCAGGAAAATTGCTTTCTGTTTTTAGTCGTTTATATACTGTTTATTTACAGATGCATGGGTTCACTTGTGGAGTTGACGATCTTTTGCTGATACAAAGCAAAGACAAAAAAATGAAGGAACAACTTGAAAGTTGTGAAGAGCTTGGTGAAAAAGTTTTCCGTGAGTTTATTGGGGTCGGACAAGATGAAAAAAAAGATCCGGTGATACTGCAGTCGGATATTGAAATTTTTTTACGCAGTAATGGAGAATCTGTGACAGAAATGTTGGACAGGAAAATGACAAGTGAACTTAATAAGAAAATCTCCAAATCTGATGTCTTCAAACAATTGTTTCGGAAAGGGTTATCAAAGCCATCTGTTAAGAATTATATGTATCTTATGACAAGCTCTGGGGCTAAAGGAGGGGTGGCAAATCTCCAACAAATTGCTTCATACTTGGGTCAGCAAGAGCTAGAAGGGAAACGAGTCCCTCAAATGGTTTCTGGAAAGACATTACCATGCTTCCCTCCTTGGGATTGGTCACCTCGAGCTGGAGGTTTTATTATTGATCGCTTTCTTACCGGTCTTCATCCTCAGGAGTATTATTTTCACTGCATGGCTGGCCGTGAAGGGTTGGTTGATACAGCTGTCAAAACATCTCGTAGTGGATACTTGCAAAGATGCCTAATAAAAAATCTTGAATGCCTCAAGGTTTCTTATGACCATACTGTCCGTGATGCTGATGGTTCAATTATTCAATTCCGATATGGAGAGGATGGTATTGATGTCCATTTGACAAGCTTTATTAAAAAATTTGAAAAACTGGCAAATAATAAAGAAATGTTTGACAAGAAATTTGGTCGTCAAATTGACAAATTCAATCCCTATATTGATGAGTTGCCTCCCGCTCTCAAAAGAAAAGCAGAAATGTCAGTTCCTAATTTGTCTTTGAAGGAGAAAAATGCCAACTTGAAGTTAATGGAGCACAAATATCTCAGCAGTCTTGCGCAACCAGGAGAACCAGTTGGTATTCTTGCTGCTCAGTCTGTAGGAGAACCATCAACACAGATGACTTTGAATACCTTCCATAATGCTGGGCGAAGTGAAATGAATGTGACTCTTGGAATTCCACGGCTCCAAGAGATCTTGATGACTGCTTCAAATGATATCAAGACTCCAGTTATGACATGTCCATTGATAAAGGAGAAAGCTACATTCTGTAATGTTCTCCATCTCAAATTGATATTTGCAATTTGCTATGTGCAGGGGGATGCTAAAAGTCTAGCTGATAGGTTGAAGAGGATCACTGTGGCAGATTTAATAGAGAGCATGAAAGTAACTATTACACCATTCACAGATCAGTATCATCAGACTTGCCGTACTTATGAACTGGAGATGACACTTTTTATACCCGAATATCTTTCGAAATTTTCAGAAGATTGGGAGGAACTCCTAGAGGTTAAGTTTGTTAGGGCTTTGGAGGATGCCATACAAAATCATCTGGTTTTGCTTTCTAAAATTAGTGGTATAAAAGATGTCAAGGATGATTCACTACCGAAGGCATCAAATGAGACAGATGAAGATGTTTCTTACAACATATCCCAGCAGGGTGTAGAAGATGATGACGATGGCGGTGCAGATGACAGTGAACATGGTGAAGGAGCTGAGGATTTTGGGTTGGATGCACAAAAACGAAAGCGGCAGGGCACAGATGAGATGGAGTATGAAGATGACTGTGAGGATGAAGTGAATGAAGGTCATCAGTCCGATGGATATGAAAGCGAAATTGATAAGGTGGAAAGTGATATTGAAAGTGATAAGGATGGTGTGACGCAGATGTCAGATGCTAATGATGAAATGCCTGAAAGTCCTTCGGCTGGAGACACATCTAAGCCACGGTCGAAGGGAAAGGGGACCAAATCAAGGGACAAAATTAAAAAGAAGACCAGAGCAAAGCTAGTAAGAAAGCAGTATGACAGGGCAACTTTTGTGTCAGTCAAGGGGTTCCACTTTGAGATCCATTTTAAATTTACCAATGAACCTCATATTCTTTTGGATCAGATTGCTCGTAAGACAGCTCGAAATGTTAATGTCAAAAGCTCTGGCAATATTTTGGATTGTAAAGATATTGATATTTATGGTAATGAAGATCAAGTTTTCTGCTCCTGGAATGATCCCAGAAATAAATTACCGGGTGAAAAGAACCCCACGGAAGAAGAAAAGAAGATGCGAGCCTTGCAGACAGCAGGGGTGGATTTTGCTACACTATGGAAACTGCAAGATGATCTTGATGTCAACTATATATATTCCAACAACATTCATGCAATGCTGAACACTTATGGAGTAGAAGCCGCCAGAGAAACTATCATCAAAGAAATAAGTAACGTCTTCAAGGTTTATGGGATATCTGTTAATATTAGACACCTTTCACTAATAGCTGACTACATGACTCATAGTGGCGGTTATCGTCCAATGAACAGATTTGGGGGTATAGCAGAGTCTGTATCTCCGATCAATAAAATGTCATTTGAGACGGCTTCCAAGTTCATTCGTGAAGCAGCATATCATGGGGAGACGGATGACTTGGAAACCCCTTCTGCTAGGATCTGTCTTGGGCTGCCAGTGAAAGTGGGTACTGGTTGTTTTGATTTGATGCAAAAGATGGAGATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1565

Amino Acids

176.07

Weight (kDa)

6.23

Isoelectric Point (pI)

43.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	52 - 300	1.3e-07	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	303 - 484	4e-58	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	488 - 710	1.7e-29	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	764 - 863	7.1e-20	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	870 - 1504	5.2e-83	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1520
AasI	GACNNNNNNGTC	2 cut(s)	2753, 3862
Acc16I	TGCGCA	1 cut(s)	3033
Acc36I	ACCTGC	1 cut(s)	1717
AccBSI	CCGCTC	2 cut(s)	1560, 2933
AccI	GTMKAC	1 cut(s)	1858
AccII	CGCG	1 cut(s)	1428
AciI	CCGC	9 cut(s)	20, 827, 1132, 1558, 2931, 3657, 3724, 4377, 4485
AcoI	YGGCCR	1 cut(s)	2647
AcuI	CTGAAG	3 cut(s)	119, 1452, 1683
AfaI	GTAC	5 cut(s)	627, 1227, 1363, 3406, 4660
AfeI	AGCGCT	2 cut(s)	1142, 1525
AfiI	CCNNNNNNNGG	7 cut(s)	236, 1481, 2521, 2557, 2576, 3902, 3948
AflIII	ACRYGT	1 cut(s)	3212
AgeI	ACCGGT	1 cut(s)	2606
AhlI	ACTAGT	1 cut(s)	1478
AjnI	CCWGG	5 cut(s)	495, 1081, 1633, 3037, 4190
AloI	GAACNNNNNNTCC	2 cut(s)	872, 904
Alw21I	GWGCWC	1 cut(s)	3012
Alw26I	GTCTC	6 cut(s)	93, 425, 3590, 3900, 4550, 4592
AlwNI	CAGNNNCTG	2 cut(s)	868, 4526
Ama87I	CYCGRG	2 cut(s)	475, 2571
Aor51HI	AGCGCT	2 cut(s)	1142, 1525
AoxI	GGCC	1 cut(s)	2647
ApeKI	GCWGC	5 cut(s)	1675, 2045, 3062, 4583, 4642
ArsI	GACNNNNNNTTYG	4 cut(s)	2346, 2378, 2864, 2896
AsiGI	ACCGGT	1 cut(s)	2606
Asp700I	GAANNNNTTC	2 cut(s)	1037, 3107
AspLEI	GCGC	4 cut(s)	1143, 1430, 1526, 3034
AspS9I	GGNCC	5 cut(s)	305, 1510, 1966, 2011, 3939
AsuC2I	CCSGG	1 cut(s)	4218
AsuII	TTCGAA	2 cut(s)	110, 3449
AvaI	CYCGRG	2 cut(s)	475, 2571
AvaII	GGWCC	5 cut(s)	305, 1510, 1966, 2011, 3939
AxyI	CCTNAGG	1 cut(s)	2619
BaeGI	GKGCMC	2 cut(s)	444, 3734
BaeI	ACNNNNGTAYC	4 cut(s)	2243, 2276, 2658, 2691
BamHI	GGATCC	1 cut(s)	1924
BbsI	GAAGAC	5 cut(s)	1671, 2356, 2603, 3974, 4405
Bbv12I	GWGCWC	1 cut(s)	3012
BbvCI	CCTCAGC	1 cut(s)	3690
BbvI	GCAGC	5 cut(s)	1687, 2032, 3049, 4595, 4629
BceAI	ACGGC	4 cut(s)	2634, 3173, 3387, 4575
BcgI	CGANNNNNNTGC	2 cut(s)	3641, 3675
BciT130I	CCWGG	5 cut(s)	497, 1083, 1635, 3039, 4192
BciVI	GTATCC	1 cut(s)	2687
BcnI	CCSGG	1 cut(s)	4218
BcoDI	GTCTC	6 cut(s)	93, 425, 3590, 3900, 4550, 4592
BcuI	ACTAGT	1 cut(s)	1478
BfaI	CTAG	8 cut(s)	270, 611, 1479, 2498, 3308, 3479, 3983, 4626
BfmI	CTRYAG	6 cut(s)	1056, 1673, 1951, 2046, 2255, 3072
BfoI	RGCGCY	2 cut(s)	1144, 1527
BfuAI	ACCTGC	1 cut(s)	1717
BfuI	GTATCC	1 cut(s)	2687
BglII	AGATCT	2 cut(s)	481, 3168
BisI	GCNGC	8 cut(s)	21, 1676, 2046, 3063, 3725, 4377, 4584, 4643
BlsI	GCNGC	8 cut(s)	22, 1677, 2047, 3064, 3726, 4378, 4585, 4644
Bme1390I	CCNGG	6 cut(s)	497, 1083, 1635, 3039, 4192, 4218
Bme18I	GGWCC	5 cut(s)	305, 1510, 1966, 2011, 3939
BmeT110I	CYCGRG	2 cut(s)	475, 2571
BmgT120I	GGNCC	5 cut(s)	305, 1510, 1966, 2011, 3939
BmiI	GGNNCC	4 cut(s)	1926, 3161, 3940, 4031
BmrFI	CCNGG	6 cut(s)	497, 1083, 1635, 3039, 4192, 4218
BmrI	ACTGGG	2 cut(s)	438, 1284
BmuI	ACTGGG	2 cut(s)	438, 1284
BpiI	GAAGAC	5 cut(s)	1671, 2356, 2603, 3974, 4405
BplI	GAGNNNNNCTC	2 cut(s)	1009, 1041
BpmI	CTGGAG	8 cut(s)	194, 633, 890, 1104, 2598, 3186, 3437, 3924
Bpu10I	CCTNAGC	1 cut(s)	3690
Bpu14I	TTCGAA	2 cut(s)	110, 3449
BpuEI	CTTGAG	2 cut(s)	381, 2717
BpuMI	CCSGG	1 cut(s)	4218
BsaAI	YACGTR	1 cut(s)	1468
BsaBI	GATNNNNATC	2 cut(s)	127, 3387
BsaI	GGTCTC	1 cut(s)	425
BsaJI	CCNNGG	8 cut(s)	495, 571, 1028, 1969, 2555, 3155, 3920, 4233
BsaWI	WCCGGW	2 cut(s)	2245, 2606
BsaXI	ACNNNNNCTCC	6 cut(s)	1130, 1160, 2283, 2313, 3033, 3063
Bsc4I	CCNNNNNNNGG	7 cut(s)	236, 1481, 2521, 2557, 2576, 3902, 3948
Bse118I	RCCGGY	2 cut(s)	930, 2606
Bse21I	CCTNAGG	1 cut(s)	2619
Bse3DI	GCAATG	3 cut(s)	156, 271, 4356
Bse8I	GATNNNNATC	2 cut(s)	127, 3387
BseBI	CCWGG	5 cut(s)	497, 1083, 1635, 3039, 4192
BseDI	CCNNGG	8 cut(s)	495, 571, 1028, 1969, 2555, 3155, 3920, 4233
BseJI	GATNNNNATC	2 cut(s)	127, 3387
BseLI	CCNNNNNNNGG	7 cut(s)	236, 1481, 2521, 2557, 2576, 3902, 3948
BseMI	GCAATG	3 cut(s)	156, 271, 4356
BseMII	CTCAG	7 cut(s)	698, 1005, 1575, 2633, 3034, 3080, 3681
BseRI	GAGGAG	1 cut(s)	1523
BseSI	GKGCMC	2 cut(s)	444, 3734
BseXI	GCAGC	5 cut(s)	1687, 2032, 3049, 4595, 4629
BseYI	CCCAGC	2 cut(s)	3119, 3627
BsgI	GTGCAG	3 cut(s)	2042, 3308, 3681
Bsh1236I	CGCG	1 cut(s)	1428
Bsh1285I	CGRYCG	1 cut(s)	3926
BshFI	GGCC	1 cut(s)	2649
BshTI	ACCGGT	1 cut(s)	2606
BsiEI	CGRYCG	1 cut(s)	3926
BsiHKAI	GWGCWC	1 cut(s)	3012
BsiHKCI	CYCGRG	2 cut(s)	475, 2571
BsiSI	CCGG	5 cut(s)	116, 931, 2246, 2607, 4217
BslFI	GGGAC	5 cut(s)	1198, 1262, 2498, 3952, 3965
BslI	CCNNNNNNNGG	7 cut(s)	236, 1481, 2521, 2557, 2576, 3902, 3948
BsmAI	GTCTC	6 cut(s)	93, 425, 3590, 3900, 4550, 4592
BsmBI	CGTCTC	2 cut(s)	4550, 4592
BsmFI	GGGAC	5 cut(s)	1198, 1262, 2498, 3952, 3965
BsmI	GAATGC	3 cut(s)	106, 1057, 2732
BsnI	GGCC	1 cut(s)	2649
Bso31I	GGTCTC	1 cut(s)	425
BsoBI	CYCGRG	2 cut(s)	475, 2571
Bsp119I	TTCGAA	2 cut(s)	110, 3449
Bsp1286I	GDGCHC	3 cut(s)	444, 3012, 3734
Bsp19I	CCATGG	1 cut(s)	1028
BspACI	CCGC	9 cut(s)	20, 827, 1132, 1558, 2931, 3657, 3724, 4377, 4485
BspANI	GGCC	1 cut(s)	2649
BspCNI	CTCAG	7 cut(s)	697, 1006, 1574, 2632, 3033, 3079, 3682
BspFNI	CGCG	1 cut(s)	1428
BspLI	GGNNCC	4 cut(s)	1926, 3161, 3940, 4031
BspMAI	CTGCAG	3 cut(s)	1677, 2050, 2259
BspMI	ACCTGC	1 cut(s)	1717
BspQI	GCTCTTC	1 cut(s)	2178
BspT104I	TTCGAA	2 cut(s)	110, 3449
BspTNI	GGTCTC	1 cut(s)	425
BsrBI	CCGCTC	2 cut(s)	1560, 2933
BsrDI	GCAATG	3 cut(s)	156, 271, 4356
BsrFI	RCCGGY	2 cut(s)	930, 2606
BssAI	RCCGGY	2 cut(s)	930, 2606
BssECI	CCNNGG	8 cut(s)	495, 571, 1028, 1969, 2555, 3155, 3920, 4233
BssT1I	CCWWGG	3 cut(s)	571, 1028, 2555
Bst2UI	CCWGG	5 cut(s)	497, 1083, 1635, 3039, 4192
Bst6I	CTCTTC	7 cut(s)	138, 318, 410, 1833, 1884, 2178, 3317
BstBAI	YACGTR	1 cut(s)	1468
BstBI	TTCGAA	2 cut(s)	110, 3449
BstDEI	CTNAG	9 cut(s)	554, 684, 1014, 1561, 2619, 3020, 3066, 3690, 3915
BstDSI	CCRYGG	5 cut(s)	1028, 1969, 3155, 3920, 4233
BstEII	GGTNACC	1 cut(s)	2565
BstFNI	CGCG	1 cut(s)	1428
BstH2I	RGCGCY	2 cut(s)	1144, 1527
BstHHI	GCGC	4 cut(s)	1143, 1430, 1526, 3034
BstMAI	GTCTC	6 cut(s)	93, 425, 3590, 3900, 4550, 4592
BstMCI	CGRYCG	1 cut(s)	3926
BstMWI	GCNNNNNNNGC	7 cut(s)	112, 266, 778, 1129, 1138, 2646, 4261
BstNI	CCWGG	5 cut(s)	497, 1083, 1635, 3039, 4192
BstNSI	RCATGY	4 cut(s)	889, 1345, 1608, 3216
BstPI	GGTNACC	1 cut(s)	2565
BstSCI	CCNGG	6 cut(s)	495, 1081, 1633, 3037, 4190, 4216
BstSFI	CTRYAG	6 cut(s)	1056, 1673, 1951, 2046, 2255, 3072
BstSLI	GKGCMC	2 cut(s)	444, 3734
BstSNI	TACGTA	1 cut(s)	1468
BstUI	CGCG	1 cut(s)	1428
BstV1I	GCAGC	5 cut(s)	1687, 2032, 3049, 4595, 4629
BstV2I	GAAGAC	5 cut(s)	1671, 2356, 2603, 3974, 4405
BstX2I	RGATCY	8 cut(s)	481, 949, 1202, 1924, 2242, 3168, 4041, 4629
BstXI	CCANNNNNNTGG	2 cut(s)	1297, 3119
BstYI	RGATCY	8 cut(s)	481, 949, 1202, 1924, 2242, 3168, 4041, 4629
Bsu36I	CCTNAGG	1 cut(s)	2619
BsuI	GTATCC	1 cut(s)	2687
BsuRI	GGCC	1 cut(s)	2649
BtgI	CCRYGG	5 cut(s)	1028, 1969, 3155, 3920, 4233
BtsI	GCAGTG	2 cut(s)	1670, 2635
BtsIMutI	CAGTG	7 cut(s)	451, 1670, 1710, 2635, 3330, 3676, 4653
BveI	ACCTGC	1 cut(s)	1717
CaiI	CAGNNNCTG	2 cut(s)	868, 4526
CfoI	GCGC	4 cut(s)	1143, 1430, 1526, 3034
Cfr10I	RCCGGY	2 cut(s)	930, 2606
Cfr13I	GGNCC	5 cut(s)	305, 1510, 1966, 2011, 3939
CseI	GACGC	3 cut(s)	743, 1697, 3865
Csp6I	GTAC	5 cut(s)	626, 1226, 1362, 3405, 4659
CspAI	ACCGGT	1 cut(s)	2606
CviQI	GTAC	5 cut(s)	626, 1226, 1362, 3405, 4659
DdeI	CTNAG	9 cut(s)	554, 684, 1014, 1561, 2619, 3020, 3066, 3690, 3915
DraI	TTTAAA	1 cut(s)	4051
DrdI	GACNNNNNNGTC	2 cut(s)	2753, 3862
DseDI	GACNNNNNNGTC	2 cut(s)	2753, 3862
EaeI	YGGCCR	1 cut(s)	2647
Eam1104I	CTCTTC	7 cut(s)	138, 318, 410, 1833, 1884, 2178, 3317
EarI	CTCTTC	7 cut(s)	138, 318, 410, 1833, 1884, 2178, 3317
Eco105I	TACGTA	1 cut(s)	1468
Eco130I	CCWWGG	3 cut(s)	571, 1028, 2555
Eco31I	GGTCTC	1 cut(s)	425
Eco32I	GATATC	3 cut(s)	562, 3193, 4431
Eco47I	GGWCC	5 cut(s)	305, 1510, 1966, 2011, 3939
Eco47III	AGCGCT	2 cut(s)	1142, 1525
Eco57I	CTGAAG	3 cut(s)	119, 1452, 1683
Eco81I	CCTNAGG	1 cut(s)	2619
Eco88I	CYCGRG	2 cut(s)	475, 2571
Eco91I	GGTNACC	1 cut(s)	2565
EcoO109I	RGGNCCY	1 cut(s)	305
EcoO65I	GGTNACC	1 cut(s)	2565
EcoRI	GAATTC	2 cut(s)	978, 3150
EcoRII	CCWGG	5 cut(s)	495, 1081, 1633, 3037, 4190
EcoRV	GATATC	3 cut(s)	562, 3193, 4431
EcoT14I	CCWWGG	3 cut(s)	571, 1028, 2555
EcoT22I	ATGCAT	1 cut(s)	2103
ErhI	CCWWGG	3 cut(s)	571, 1028, 2555
Esp3I	CGTCTC	2 cut(s)	4550, 4592
FalI	AAGNNNNNCTT	6 cut(s)	723, 755, 1535, 1567, 4296, 4328
FaqI	GGGAC	5 cut(s)	1198, 1262, 2498, 3952, 3965
FauI	CCCGC	2 cut(s)	820, 2938
FblI	GTMKAC	1 cut(s)	1858
Fnu4HI	GCNGC	8 cut(s)	21, 1676, 2046, 3063, 3725, 4377, 4584, 4643
Fsp4HI	GCNGC	8 cut(s)	21, 1676, 2046, 3063, 3725, 4377, 4584, 4643
FspBI	CTAG	8 cut(s)	270, 611, 1479, 2498, 3308, 3479, 3983, 4626
FspI	TGCGCA	1 cut(s)	3033
GlaI	GCGC	4 cut(s)	1142, 1429, 1525, 3033
GluI	GCNGC	8 cut(s)	21, 1676, 2046, 3063, 3725, 4377, 4584, 4643
GsaI	CCCAGC	2 cut(s)	3123, 3631
GsuI	CTGGAG	8 cut(s)	194, 633, 890, 1104, 2598, 3186, 3437, 3924
HaeII	RGCGCY	2 cut(s)	1144, 1527
HaeIII	GGCC	1 cut(s)	2649
HapII	CCGG	5 cut(s)	116, 931, 2246, 2607, 4217
HgaI	GACGC	3 cut(s)	743, 1697, 3865
HhaI	GCGC	4 cut(s)	1143, 1430, 1526, 3034
Hin6I	GCGC	4 cut(s)	1141, 1428, 1524, 3032
HinP1I	GCGC	4 cut(s)	1141, 1428, 1524, 3032
HincII	GTYRAC	3 cut(s)	1859, 2122, 4321
HindII	GTYRAC	3 cut(s)	1859, 2122, 4321
HindIII	AAGCTT	2 cut(s)	1434, 2824
HpaII	CCGG	5 cut(s)	116, 931, 2246, 2607, 4217
Hpy99I	CGWCG	1 cut(s)	1863
HpyCH4IV	ACGT	5 cut(s)	1467, 1652, 1739, 2019, 4410
HpyF10VI	GCNNNNNNNGC	7 cut(s)	112, 266, 778, 1129, 1138, 2646, 4261
HpyF3I	CTNAG	9 cut(s)	554, 684, 1014, 1561, 2619, 3020, 3066, 3690, 3915
HpySE526I	ACGT	5 cut(s)	1467, 1652, 1739, 2019, 4410
HspAI	GCGC	4 cut(s)	1141, 1428, 1524, 3032
LguI	GCTCTTC	1 cut(s)	2178
LmnI	GCTCC	7 cut(s)	676, 1085, 1138, 3007, 3165, 3686, 4193
Lsp1109I	GCAGC	5 cut(s)	1687, 2032, 3049, 4595, 4629
MaeI	CTAG	8 cut(s)	270, 611, 1479, 2498, 3308, 3479, 3983, 4626
MaeII	ACGT	5 cut(s)	1467, 1652, 1739, 2019, 4410
MbiI	CCGCTC	2 cut(s)	1560, 2933
MfeI	CAATTG	1 cut(s)	2372
MflI	RGATCY	8 cut(s)	481, 949, 1202, 1924, 2242, 3168, 4041, 4629
MhlI	GDGCHC	3 cut(s)	444, 3012, 3734
MlyI	GAGTC	9 cut(s)	29, 35, 388, 1610, 2520, 3136, 3193, 4468, 4532
MmeI	TCCRAC	7 cut(s)	755, 2203, 2241, 2292, 2488, 3684, 4359
Mph1103I	ATGCAT	1 cut(s)	2103
MroXI	GAANNNNTTC	2 cut(s)	1037, 3107
MslI	CAYNNNNRTG	2 cut(s)	660, 2542
MspA1I	CMGCKG	1 cut(s)	2672
MspI	CCGG	5 cut(s)	116, 931, 2246, 2607, 4217
MspR9I	CCNGG	6 cut(s)	497, 1083, 1635, 3039, 4192, 4218
MunI	CAATTG	1 cut(s)	2372
Mva1269I	GAATGC	3 cut(s)	106, 1057, 2732
MvaI	CCWGG	5 cut(s)	497, 1083, 1635, 3039, 4192
MvnI	CGCG	1 cut(s)	1428
MwoI	GCNNNNNNNGC	7 cut(s)	112, 266, 778, 1129, 1138, 2646, 4261
NciI	CCSGG	1 cut(s)	4218
NcoI	CCATGG	1 cut(s)	1028
NlaIV	GGNNCC	4 cut(s)	1926, 3161, 3940, 4031
NmuCI	GTSAC	7 cut(s)	391, 593, 1232, 2299, 2565, 3139, 3853
NsbI	TGCGCA	1 cut(s)	3033
NsiI	ATGCAT	1 cut(s)	2103
NspI	RCATGY	4 cut(s)	889, 1345, 1608, 3216
NspV	TTCGAA	2 cut(s)	110, 3449
PaeI	GCATGC	1 cut(s)	1345
PaeR7I	CTCGAG	1 cut(s)	2571
PciI	ACATGT	1 cut(s)	3212
PciSI	GCTCTTC	1 cut(s)	2178
PctI	GAATGC	3 cut(s)	106, 1057, 2732
PdmI	GAANNNNTTC	2 cut(s)	1037, 3107
PfeI	GAWTC	8 cut(s)	211, 332, 665, 1504, 1825, 1907, 2294, 3572
PfoI	TCCNGGA	2 cut(s)	1081, 4190
PinAI	ACCGGT	1 cut(s)	2606
PkrI	GCNGC	8 cut(s)	22, 1677, 2047, 3064, 3726, 4378, 4585, 4644
PleI	GAGTC	9 cut(s)	29, 35, 388, 1610, 2519, 3136, 3193, 4468, 4531
PpsI	GAGTC	9 cut(s)	29, 35, 388, 1610, 2519, 3136, 3193, 4468, 4531
Ppu21I	YACGTR	1 cut(s)	1468
PpuMI	RGGWCCY	1 cut(s)	305
PscI	ACATGT	1 cut(s)	3212
PsiI	TTATAA	1 cut(s)	1520
Psp5II	RGGWCCY	1 cut(s)	305
Psp6I	CCWGG	5 cut(s)	495, 1081, 1633, 3037, 4190
PspEI	GGTNACC	1 cut(s)	2565
PspFI	CCCAGC	2 cut(s)	3119, 3627
PspGI	CCWGG	5 cut(s)	495, 1081, 1633, 3037, 4190
PspN4I	GGNNCC	4 cut(s)	1926, 3161, 3940, 4031
PspPI	GGNCC	5 cut(s)	305, 1510, 1966, 2011, 3939
PspPPI	RGGWCCY	1 cut(s)	305
PspXI	VCTCGAGB	1 cut(s)	2571
PstI	CTGCAG	3 cut(s)	1677, 2050, 2259
PstNI	CAGNNNCTG	2 cut(s)	868, 4526
PsuI	RGATCY	8 cut(s)	481, 949, 1202, 1924, 2242, 3168, 4041, 4629
PvuII	CAGCTG	1 cut(s)	2672
RsaI	GTAC	5 cut(s)	627, 1227, 1363, 3406, 4660
RsaNI	GTAC	5 cut(s)	626, 1226, 1362, 3405, 4659
RseI	CAYNNNNRTG	2 cut(s)	660, 2542
SalI	GTCGAC	1 cut(s)	1857
SapI	GCTCTTC	1 cut(s)	2178
SatI	GCNGC	8 cut(s)	21, 1676, 2046, 3063, 3725, 4377, 4584, 4643
Sau96I	GGNCC	5 cut(s)	305, 1510, 1966, 2011, 3939
SchI	GAGTC	9 cut(s)	29, 35, 388, 1610, 2520, 3136, 3193, 4468, 4532
ScrFI	CCNGG	6 cut(s)	497, 1083, 1635, 3039, 4192, 4218
SduI	GDGCHC	3 cut(s)	444, 3012, 3734
SfcI	CTRYAG	6 cut(s)	1056, 1673, 1951, 2046, 2255, 3072
Sfr274I	CTCGAG	1 cut(s)	2571
SfuI	TTCGAA	2 cut(s)	110, 3449
SinI	GGWCC	5 cut(s)	305, 1510, 1966, 2011, 3939
SlaI	CTCGAG	1 cut(s)	2571
SmiMI	CAYNNNNRTG	2 cut(s)	660, 2542
SmlI	CTYRAG	3 cut(s)	396, 2571, 2732
SmoI	CTYRAG	3 cut(s)	396, 2571, 2732
SnaBI	TACGTA	1 cut(s)	1468
SpeI	ACTAGT	1 cut(s)	1478
SphI	GCATGC	1 cut(s)	1345
SsiI	CCGC	9 cut(s)	20, 827, 1132, 1558, 2931, 3657, 3724, 4377, 4485
SspI	AATATT	2 cut(s)	4135, 4441
SspMI	CTAG	8 cut(s)	270, 611, 1479, 2498, 3308, 3479, 3983, 4626
StyD4I	CCNGG	6 cut(s)	495, 1081, 1633, 3037, 4190, 4216
StyI	CCWWGG	3 cut(s)	571, 1028, 2555
TaiI	ACGT	5 cut(s)	1470, 1655, 1742, 2022, 4413
TaqI	TCGA	8 cut(s)	110, 810, 1495, 1858, 2572, 3449, 3926, 4105
TatI	WGTACW	2 cut(s)	1225, 1361
TauI	GCSGC	3 cut(s)	23, 3727, 4379
TfiI	GAWTC	8 cut(s)	211, 332, 665, 1504, 1825, 1907, 2294, 3572
TscAI	CASTG	7 cut(s)	451, 1677, 1710, 2642, 3337, 3676, 4653
TseFI	GTSAC	7 cut(s)	391, 593, 1232, 2299, 2565, 3139, 3853
TseI	GCWGC	5 cut(s)	1675, 2045, 3062, 4583, 4642
Tsp45I	GTSAC	7 cut(s)	391, 593, 1232, 2299, 2565, 3139, 3853
TspGWI	ACGGA	7 cut(s)	881, 1220, 1958, 2195, 2747, 4250, 4616
TspRI	CASTG	7 cut(s)	451, 1677, 1710, 2642, 3337, 3676, 4653
VpaK11BI	GGWCC	5 cut(s)	305, 1510, 1966, 2011, 3939
XceI	RCATGY	4 cut(s)	889, 1345, 1608, 3216
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1710, 1757
XhoI	CTCGAG	1 cut(s)	2571
XmiI	GTMKAC	1 cut(s)	1858
XmnI	GAANNNNTTC	2 cut(s)	1037, 3107
XspI	CTAG	8 cut(s)	270, 611, 1479, 2498, 3308, 3479, 3983, 4626
Zsp2I	ATGCAT	1 cut(s)	2103

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.