Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00321

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Forward (+)

250 .. 6242

Gene Structure 5993 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G008430.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2916 bp

GTCATCACTACGTTGCTAAACCATATTACAAGTGGTTCTCCACCACTTACTGTTGAAAAGGAAGCAAAACTCCCAGCTGGGTTTTTCAACTGTAAGAATCTTGAAGAAGAAGAGAAATCTGATAAAGGTCGACAAAAGAAAGAGAAGAAGCCAAGAAAAGAAGAGGCTGGTAAAGATGATTCAAAGGAAAAAGAGTATCCAGATGAGAGCAAGCTGTTAATCTATAGAAACAACTTGGTCCGTGGCGTGATTGACAAGAACCAGTTTGGTGATTATGGTTTGGTCCATAACGTGCAGGAACTCTATGGTTCGGAAGCTGCAGGAAAATTGCTTTCTGTTTTTAGTCGTTTATATACTGTTTATTTACAGATGCATGGGTTCACTTGTGGAGTTGACGATCTTTTGCTAATACAAAGCAAAGACAAAAAAATGAAGGAACAACTTGAAAGTTGTGAAGAGCTTGGTGAAAAAGTTTTCCGTGAGTTTATTGGGGTTGGACAAGATGAAAAAAAAGATCCGGTGATACTGCAGTCGGATATTGAAGAATTTTTACGCAGTAATGGAGAATCTGTGACGGAAATGTTGGACAGGAAAATGACAAGTGAACTTAATAAGAAAATCTCCAAATCTGATGTCTTCAAACAATTGTTTCAGAAAGGGTTATCAAAGCCATCTGTTAAGAATTATATGTATCTTATGACAAGCTCTGGGGCTAAAGGAGGTGTGGCAAATCTCCAACAAATTGCTTCATACTTGGGTCAGCAAGAGCTAGAAGGGAAACGAGTCCCTCAAATGGTTTCTGGAAAGACATTACCATGCTTCCCTCCTTGGGATTGGTCACCTCGAGCTGGAGGTTTTATTATTGATCGCTTTCTCACCGGTCTTCATCCTCAGGAGTATTATTTTCACTGCATGGCTGGCCGTGAAGGGTTGGTTGATACAGCTGTCAAAACATCTCGTAGTGGATACTTGCAAAGATGCCTAATAAAAAATCTCGAATGCCTCAAGGTTTCTTATGACCATACTGTCCGTGATGCTGATGGTTCAATTATTCAATTCCGATATGGAGAGGATGGTATTGATGTCCATTTGACAAGCTTTATTAAAAAATTTGAAAAACTGGCAAATAATAAAGAAATGTTTGACAAGAAATTTGGTCGTCAAATTGACAAATTCAATCCCTATATTGATGAGTTGCCTCCCGCTCTCAAAAGAAAAGCAGAAATGTCAGTTCCTAATTTGTCTTTGAAGGAGAAAAATGCCAACTTGAAGTTAATGGAGCACAAATATCTCAGCAGTCTTGCTCAACCAGGAGAACCAGTTGGTATTCTTGCTGCTCAGTCTGTAGGAGAACCATCAACACAGATGACTTTGAATACCTTCCATAATGCTGGGCGAAGTGAAATGAATGTGACTCTTGGAATTCCACGGCTCCAAGAGATCTTGATGACTGCTTCAAATGATATCAAGACTCCAGTTATGACATGTCCATTGATAAAGGAGAAAGCGGGGGATGCTAAAAGTCTAGCTGATAGGTTGAAGAGGATCACTGTGGCAGACATAATAGAAAGCATGAAAGTAACTATTACACCATTCACAGATCAGTATCATCAGACTTGCCGTACTTATGAACTGGAGATGACACTTTTTATACCAGAATATCTTTCGAAATTTTCAGAAGATTGGGAGGAGCTCCTAGAGGTTAAGTTTGTTAGGGCTTTGGAGGATGCCATACAAAATCATCTGGTTTTGCTTTCTAAAATTAGTGGTATAAAAGATGTCAAGGCTGATTCACTACCGAAGGCATCAAATGAGACAGATGAAGATGTTTCTTACAACATATCCCAGCATGGTGTAGAAGATGATGACGATGGCGGTGCAGATGACAGTGAACATGGTGAAGGAGCTGAGGATTTTGGGTTGGATGCACAAAAACGAAAGCGGCAGGGCACAGATGAGATGGAATATGAAGATGACTGTGAGGATGAAGTGAATGAAGGTCATCAGTCCGATGGATTTGAAAGCGAAATTGATAAGGTGGAAAGTGATATTGAAAGTGATAAGGATGGTGTGACGCGGATTTCAGATGCTAATGATGAAATGCCTGAAAGTCCTTCAGCTGGAGACACATCTAAGCCACGGTCGAAGGGAAAGAAGACCAAATCAAGGGACAAAATTAAAAAGAAGACCAGAGCAAAGCTAGTAAGAAAGGAGTATGACAGGGCAACTTTTGTGTCAGTCAAGGGGTTCCACTTTGAGATCCATTTTAAATTTACCAATGAACCTCATATTCTTTTGGATCAGATTGCTCGGAAGACAGCTCGAAATGTTTATGTCAAAAGCTCTGGCAATATTTTGGATTGTAAAGATATTGATATTTATGGTAATGAAGATCAAGTTTTCTGCTCCTGGAATGATCCCAGAAATAAATTACCGGGTGAAAAGAATCCCACGGAAGAAGAAAAGAAGATGCGAGCCTTGCAGACAGCAGGGTTGGATTTTGCTACACTATGGAAACTGCAAGATGATCTTGATGTCAACTATATATATTCCAACAACATTCATGCAATGCTGAACACTTATGGAGTAGAAGCCGCCAGAGAAACGATCATCAAAGAAATAAGTAACGTCTTCAAGGTTTATGGGATATCTGTCAATATTAGACACCTTTCACTAATAGCTGACTACATGACTCATAGTGGCGGTTATCGTCCAATGAACAGATTTGGGGGTATAGCAGAGTCTGTATCTCCGATCAATAAAATGTCATTTGAGACGGCTTCCAAGTTCATTCGTGAAGCAGCATATCATGGGGAGACGGATGACTTGGAAACCCCTTCTGCTAGGATCTGTCTTGGGCTGCCAGTGAAAGTGGGTACTGGTTGTTTTGATTTGATGCAAAAGATGGAGATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

971

Amino Acids

109.69

Weight (kDa)

5.55

Isoelectric Point (pI)

42.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_3	PF04983	60 - 134	2.3e-11	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	186 - 287	2.1e-20	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	294 - 910	1.2e-84	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1025
AccBSI	CCGCTC	1 cut(s)	1205
AccI	GTMKAC	1 cut(s)	130
AccII	CGCG	1 cut(s)	2077
AciI	CCGC	7 cut(s)	1203, 1508, 1875, 1942, 2077, 2595, 2703
AclWI	GGATC	6 cut(s)	509, 1553, 2254, 2307, 2411, 2855
AcoI	YGGCCR	1 cut(s)	919
AcsI	RAATTY	7 cut(s)	545, 1109, 1151, 1172, 1422, 1670, 2270
AcuI	CTGAAG	1 cut(s)	2100
AfaI	GTAC	2 cut(s)	1624, 2878
AfiI	CCNNNNNNNGG	5 cut(s)	793, 829, 848, 2120, 2166
AflIII	ACRYGT	1 cut(s)	1484
AgeI	ACCGGT	1 cut(s)	878
AjnI	CCWGG	2 cut(s)	1309, 2408
Alw21I	GWGCWC	2 cut(s)	1284, 1695
Alw26I	GTCTC	4 cut(s)	1808, 2118, 2768, 2810
AlwI	GGATC	6 cut(s)	509, 1553, 2254, 2307, 2411, 2855
AlwNI	CAGNNNCTG	1 cut(s)	2744
Ama87I	CYCGRG	1 cut(s)	843
AoxI	GGCC	1 cut(s)	919
ApeKI	GCWGC	4 cut(s)	317, 1334, 2801, 2860
ApoI	RAATTY	7 cut(s)	545, 1109, 1151, 1172, 1422, 1670, 2270
ArsI	GACNNNNNNTTYG	4 cut(s)	618, 650, 1136, 1168
AsiGI	ACCGGT	1 cut(s)	878
Asp700I	GAANNNNTTC	1 cut(s)	1379
AspS9I	GGNCC	2 cut(s)	238, 283
AsuC2I	CCSGG	1 cut(s)	2436
AsuHPI	GGTGA	7 cut(s)	281, 476, 532, 831, 868, 1910, 2450
AsuII	TTCGAA	1 cut(s)	1667
AvaI	CYCGRG	1 cut(s)	843
AvaII	GGWCC	2 cut(s)	238, 283
AxyI	CCTNAGG	1 cut(s)	891
BaeGI	GKGCMC	1 cut(s)	1952
BaeI	ACNNNNGTAYC	4 cut(s)	515, 548, 930, 963
BanII	GRGCYC	1 cut(s)	1695
BarI	GAAGNNNNNNTAC	2 cut(s)	534, 566
BbsI	GAAGAC	6 cut(s)	628, 875, 2162, 2192, 2321, 2623
Bbv12I	GWGCWC	2 cut(s)	1284, 1695
BbvCI	CCTCAGC	1 cut(s)	1908
BbvI	GCAGC	4 cut(s)	304, 1321, 2813, 2847
BccI	CCATC	9 cut(s)	679, 1034, 1067, 1363, 1865, 1954, 2006, 2060, 2899
BceAI	ACGGC	4 cut(s)	906, 1445, 1605, 2793
BcgI	CGANNNNNNTGC	2 cut(s)	1859, 1893
BciT130I	CCWGG	2 cut(s)	1311, 2410
BciVI	GTATCC	2 cut(s)	207, 959
BcnI	CCSGG	1 cut(s)	2436
BcoDI	GTCTC	4 cut(s)	1808, 2118, 2768, 2810
BfaI	CTAG	5 cut(s)	770, 1526, 1697, 2201, 2844
BfmI	CTRYAG	4 cut(s)	223, 318, 527, 1344
BfuI	GTATCC	2 cut(s)	207, 959
BglII	AGATCT	1 cut(s)	1440
BisI	GCNGC	6 cut(s)	318, 1335, 1943, 2595, 2802, 2861
BlsI	GCNGC	6 cut(s)	319, 1336, 1944, 2596, 2803, 2862
Bme1390I	CCNGG	3 cut(s)	1311, 2410, 2436
Bme18I	GGWCC	2 cut(s)	238, 283
BmeT110I	CYCGRG	1 cut(s)	843
BmgT120I	GGNCC	2 cut(s)	238, 283
BmiI	GGNNCC	2 cut(s)	1433, 2249
BmrFI	CCNGG	3 cut(s)	1311, 2410, 2436
BpiI	GAAGAC	6 cut(s)	628, 875, 2162, 2192, 2321, 2623
BpmI	CTGGAG	4 cut(s)	870, 1458, 1655, 2142
Bpu10I	CCTNAGC	1 cut(s)	1908
Bpu14I	TTCGAA	1 cut(s)	1667
BpuEI	CTTGAG	1 cut(s)	989
BpuMI	CCSGG	1 cut(s)	2436
BsaBI	GATNNNNATC	1 cut(s)	1605
BsaJI	CCNNGG	5 cut(s)	241, 827, 1427, 2138, 2451
BsaWI	WCCGGW	2 cut(s)	517, 878
BsaXI	ACNNNNNCTCC	4 cut(s)	555, 585, 1305, 1335
Bsc4I	CCNNNNNNNGG	5 cut(s)	793, 829, 848, 2120, 2166
Bse118I	RCCGGY	1 cut(s)	878
Bse1I	ACTGG	7 cut(s)	262, 1125, 1319, 1475, 1638, 2864, 2884
Bse21I	CCTNAGG	1 cut(s)	891
Bse3DI	GCAATG	1 cut(s)	2574
Bse8I	GATNNNNATC	1 cut(s)	1605
BseBI	CCWGG	2 cut(s)	1311, 2410
BseDI	CCNNGG	5 cut(s)	241, 827, 1427, 2138, 2451
BseGI	GGATG	8 cut(s)	886, 1078, 1519, 1732, 1930, 1990, 2071, 2827
BseJI	GATNNNNATC	1 cut(s)	1605
BseLI	CCNNNNNNNGG	5 cut(s)	793, 829, 848, 2120, 2166
BseMI	GCAATG	1 cut(s)	2574
BseMII	CTCAG	4 cut(s)	905, 1306, 1352, 1899
BseNI	ACTGG	7 cut(s)	262, 1125, 1319, 1475, 1638, 2864, 2884
BseRI	GAGGAG	1 cut(s)	1703
BseSI	GKGCMC	1 cut(s)	1952
BseXI	GCAGC	4 cut(s)	304, 1321, 2813, 2847
BseYI	CCCAGC	4 cut(s)	73, 77, 1391, 1845
BsgI	GTGCAG	2 cut(s)	314, 1899
Bsh1236I	CGCG	1 cut(s)	2077
Bsh1285I	CGRYCG	1 cut(s)	2144
BshFI	GGCC	1 cut(s)	921
BshTI	ACCGGT	1 cut(s)	878
BsiEI	CGRYCG	1 cut(s)	2144
BsiHKAI	GWGCWC	2 cut(s)	1284, 1695
BsiHKCI	CYCGRG	1 cut(s)	843
BsiSI	CCGG	3 cut(s)	518, 879, 2435
BslFI	GGGAC	2 cut(s)	770, 2183
BslI	CCNNNNNNNGG	5 cut(s)	793, 829, 848, 2120, 2166
BsmAI	GTCTC	4 cut(s)	1808, 2118, 2768, 2810
BsmBI	CGTCTC	2 cut(s)	2768, 2810
BsmFI	GGGAC	2 cut(s)	770, 2183
BsmI	GAATGC	1 cut(s)	1004
BsnI	GGCC	1 cut(s)	921
BsoBI	CYCGRG	1 cut(s)	843
Bsp119I	TTCGAA	1 cut(s)	1667
Bsp1286I	GDGCHC	3 cut(s)	1284, 1695, 1952
BspACI	CCGC	7 cut(s)	1203, 1508, 1875, 1942, 2077, 2595, 2703
BspANI	GGCC	1 cut(s)	921
BspCNI	CTCAG	4 cut(s)	904, 1305, 1351, 1900
BspFNI	CGCG	1 cut(s)	2077
BspLI	GGNNCC	2 cut(s)	1433, 2249
BspMAI	CTGCAG	2 cut(s)	322, 531
BspPI	GGATC	6 cut(s)	509, 1553, 2254, 2307, 2411, 2855
BspQI	GCTCTTC	1 cut(s)	450
BspT104I	TTCGAA	1 cut(s)	1667
BsrBI	CCGCTC	1 cut(s)	1205
BsrDI	GCAATG	1 cut(s)	2574
BsrFI	RCCGGY	1 cut(s)	878
BsrI	ACTGG	7 cut(s)	262, 1125, 1319, 1475, 1638, 2864, 2884
BssAI	RCCGGY	1 cut(s)	878
BssECI	CCNNGG	5 cut(s)	241, 827, 1427, 2138, 2451
BssT1I	CCWWGG	1 cut(s)	827
Bst2UI	CCWGG	2 cut(s)	1311, 2410
Bst4CI	ACNGT	8 cut(s)	52, 92, 358, 1027, 1552, 1889, 1979, 2142
Bst6I	CTCTTC	4 cut(s)	105, 156, 450, 1535
BstBI	TTCGAA	1 cut(s)	1667
BstC8I	GCNNGC	3 cut(s)	212, 919, 2475
BstDEI	CTNAG	5 cut(s)	891, 1292, 1338, 1908, 2133
BstDSI	CCRYGG	4 cut(s)	241, 1427, 2138, 2451
BstEII	GGTNACC	1 cut(s)	837
BstF5I	GGATG	8 cut(s)	886, 1078, 1519, 1732, 1930, 1990, 2071, 2827
BstFNI	CGCG	1 cut(s)	2077
BstMAI	GTCTC	4 cut(s)	1808, 2118, 2768, 2810
BstMCI	CGRYCG	1 cut(s)	2144
BstMWI	GCNNNNNNNGC	3 cut(s)	918, 1514, 2479
BstNI	CCWGG	2 cut(s)	1311, 2410
BstNSI	RCATGY	1 cut(s)	1488
BstPI	GGTNACC	1 cut(s)	837
BstSCI	CCNGG	3 cut(s)	1309, 2408, 2434
BstSFI	CTRYAG	4 cut(s)	223, 318, 527, 1344
BstSLI	GKGCMC	1 cut(s)	1952
BstUI	CGCG	1 cut(s)	2077
BstV1I	GCAGC	4 cut(s)	304, 1321, 2813, 2847
BstV2I	GAAGAC	6 cut(s)	628, 875, 2162, 2192, 2321, 2623
BstX2I	RGATCY	4 cut(s)	514, 1440, 2259, 2847
BstXI	CCANNNNNNTGG	1 cut(s)	1391
BstYI	RGATCY	4 cut(s)	514, 1440, 2259, 2847
Bsu36I	CCTNAGG	1 cut(s)	891
BsuI	GTATCC	2 cut(s)	207, 959
BsuRI	GGCC	1 cut(s)	921
BtgI	CCRYGG	4 cut(s)	241, 1427, 2138, 2451
BtsCI	GGATG	8 cut(s)	886, 1078, 1519, 1732, 1930, 1990, 2071, 2827
BtsI	GCAGTG	1 cut(s)	907
BtsIMutI	CAGTG	4 cut(s)	907, 1548, 1894, 2871
Cac8I	GCNNGC	3 cut(s)	212, 919, 2475
CaiI	CAGNNNCTG	1 cut(s)	2744
Cfr10I	RCCGGY	1 cut(s)	878
Cfr13I	GGNCC	2 cut(s)	238, 283
CseI	GACGC	1 cut(s)	2083
Csp6I	GTAC	2 cut(s)	1623, 2877
CspAI	ACCGGT	1 cut(s)	878
CviQI	GTAC	2 cut(s)	1623, 2877
DdeI	CTNAG	5 cut(s)	891, 1292, 1338, 1908, 2133
DraI	TTTAAA	1 cut(s)	2269
DrdI	GACNNNNNNGTC	1 cut(s)	1025
DseDI	GACNNNNNNGTC	1 cut(s)	1025
EaeI	YGGCCR	1 cut(s)	919
Eam1104I	CTCTTC	4 cut(s)	105, 156, 450, 1535
EarI	CTCTTC	4 cut(s)	105, 156, 450, 1535
Ecl136II	GAGCTC	1 cut(s)	1693
Eco130I	CCWWGG	1 cut(s)	827
Eco24I	GRGCYC	1 cut(s)	1695
Eco32I	GATATC	2 cut(s)	1465, 2649
Eco47I	GGWCC	2 cut(s)	238, 283
Eco53kI	GAGCTC	1 cut(s)	1693
Eco57I	CTGAAG	1 cut(s)	2100
Eco81I	CCTNAGG	1 cut(s)	891
Eco88I	CYCGRG	1 cut(s)	843
Eco91I	GGTNACC	1 cut(s)	837
EcoICRI	GAGCTC	1 cut(s)	1693
EcoO65I	GGTNACC	1 cut(s)	837
EcoRI	GAATTC	1 cut(s)	1422
EcoRII	CCWGG	2 cut(s)	1309, 2408
EcoRV	GATATC	2 cut(s)	1465, 2649
EcoT14I	CCWWGG	1 cut(s)	827
EcoT22I	ATGCAT	1 cut(s)	375
EcoT38I	GRGCYC	1 cut(s)	1695
ErhI	CCWWGG	1 cut(s)	827
Esp3I	CGTCTC	2 cut(s)	2768, 2810
FalI	AAGNNNNNCTT	2 cut(s)	2514, 2546
FaqI	GGGAC	2 cut(s)	770, 2183
FauI	CCCGC	2 cut(s)	1210, 1501
FblI	GTMKAC	1 cut(s)	130
Fnu4HI	GCNGC	6 cut(s)	318, 1335, 1943, 2595, 2802, 2861
FokI	GGATG	8 cut(s)	873, 1085, 1526, 1739, 1937, 1997, 2078, 2834
FriOI	GRGCYC	1 cut(s)	1695
Fsp4HI	GCNGC	6 cut(s)	318, 1335, 1943, 2595, 2802, 2861
FspBI	CTAG	5 cut(s)	770, 1526, 1697, 2201, 2844
GluI	GCNGC	6 cut(s)	318, 1335, 1943, 2595, 2802, 2861
GsaI	CCCAGC	4 cut(s)	77, 81, 1395, 1849
GsuI	CTGGAG	4 cut(s)	870, 1458, 1655, 2142
HaeIII	GGCC	1 cut(s)	921
HapII	CCGG	3 cut(s)	518, 879, 2435
HgaI	GACGC	1 cut(s)	2083
HincII	GTYRAC	3 cut(s)	131, 394, 2539
HindII	GTYRAC	3 cut(s)	131, 394, 2539
HindIII	AAGCTT	1 cut(s)	1096
HpaII	CCGG	3 cut(s)	518, 879, 2435
HphI	GGTGA	7 cut(s)	281, 476, 532, 831, 868, 1910, 2450
Hpy166II	GTNNAC	6 cut(s)	131, 381, 394, 605, 1892, 2539
Hpy188III	TCNNGA	9 cut(s)	101, 200, 801, 893, 995, 1444, 1468, 2531, 2795
Hpy8I	GTNNAC	6 cut(s)	131, 381, 394, 605, 1892, 2539
HpyCH4III	ACNGT	8 cut(s)	52, 92, 358, 1027, 1552, 1889, 1979, 2142
HpyCH4IV	ACGT	3 cut(s)	11, 291, 2628
HpyF10VI	GCNNNNNNNGC	3 cut(s)	918, 1514, 2479
HpyF3I	CTNAG	5 cut(s)	891, 1292, 1338, 1908, 2133
HpySE526I	ACGT	3 cut(s)	11, 291, 2628
LguI	GCTCTTC	1 cut(s)	450
LmnI	GCTCC	6 cut(s)	1279, 1437, 1690, 1698, 1904, 2411
Lsp1109I	GCAGC	4 cut(s)	304, 1321, 2813, 2847
MaeI	CTAG	5 cut(s)	770, 1526, 1697, 2201, 2844
MaeII	ACGT	3 cut(s)	11, 291, 2628
MaeIII	GTNAC	6 cut(s)	571, 837, 1411, 1579, 2071, 2624
MbiI	CCGCTC	1 cut(s)	1205
MfeI	CAATTG	1 cut(s)	644
MflI	RGATCY	4 cut(s)	514, 1440, 2259, 2847
MhlI	GDGCHC	3 cut(s)	1284, 1695, 1952
MlyI	GAGTC	5 cut(s)	792, 1408, 1465, 2686, 2750
MmeI	TCCRAC	7 cut(s)	475, 513, 564, 760, 1902, 2475, 2577
Mph1103I	ATGCAT	1 cut(s)	375
MroXI	GAANNNNTTC	1 cut(s)	1379
MseI	TTAA	9 cut(s)	218, 609, 678, 1104, 1274, 1704, 2178, 2268, 2914
MslI	CAYNNNNRTG	1 cut(s)	814
MspA1I	CMGCKG	3 cut(s)	77, 944, 2120
MspI	CCGG	3 cut(s)	518, 879, 2435
MspR9I	CCNGG	3 cut(s)	1311, 2410, 2436
MunI	CAATTG	1 cut(s)	644
Mva1269I	GAATGC	1 cut(s)	1004
MvaI	CCWGG	2 cut(s)	1311, 2410
MvnI	CGCG	1 cut(s)	2077
MwoI	GCNNNNNNNGC	3 cut(s)	918, 1514, 2479
NciI	CCSGG	1 cut(s)	2436
NlaIV	GGNNCC	2 cut(s)	1433, 2249
NmuCI	GTSAC	4 cut(s)	571, 837, 1411, 2071
NsiI	ATGCAT	1 cut(s)	375
NspI	RCATGY	1 cut(s)	1488
NspV	TTCGAA	1 cut(s)	1667
PaeR7I	CTCGAG	1 cut(s)	843
PciI	ACATGT	1 cut(s)	1484
PciSI	GCTCTTC	1 cut(s)	450
PctI	GAATGC	1 cut(s)	1004
PdmI	GAANNNNTTC	1 cut(s)	1379
PfeI	GAWTC	5 cut(s)	97, 179, 566, 1790, 2446
PfoI	TCCNGGA	1 cut(s)	2408
PinAI	ACCGGT	1 cut(s)	878
PkrI	GCNGC	6 cut(s)	319, 1336, 1944, 2596, 2803, 2862
PleI	GAGTC	5 cut(s)	791, 1408, 1465, 2686, 2749
PpsI	GAGTC	5 cut(s)	791, 1408, 1465, 2686, 2749
PscI	ACATGT	1 cut(s)	1484
Psp124BI	GAGCTC	1 cut(s)	1695
Psp6I	CCWGG	2 cut(s)	1309, 2408
PspEI	GGTNACC	1 cut(s)	837
PspFI	CCCAGC	4 cut(s)	73, 77, 1391, 1845
PspGI	CCWGG	2 cut(s)	1309, 2408
PspN4I	GGNNCC	2 cut(s)	1433, 2249
PspPI	GGNCC	2 cut(s)	238, 283
PspXI	VCTCGAGB	1 cut(s)	843
PstI	CTGCAG	2 cut(s)	322, 531
PstNI	CAGNNNCTG	1 cut(s)	2744
PsuI	RGATCY	4 cut(s)	514, 1440, 2259, 2847
PvuII	CAGCTG	3 cut(s)	77, 944, 2120
RsaI	GTAC	2 cut(s)	1624, 2878
RsaNI	GTAC	2 cut(s)	1623, 2877
RseI	CAYNNNNRTG	1 cut(s)	814
SacI	GAGCTC	1 cut(s)	1695
SalI	GTCGAC	1 cut(s)	129
SapI	GCTCTTC	1 cut(s)	450
SaqAI	TTAA	9 cut(s)	218, 609, 678, 1104, 1274, 1704, 2178, 2268, 2914
SatI	GCNGC	6 cut(s)	318, 1335, 1943, 2595, 2802, 2861
Sau96I	GGNCC	2 cut(s)	238, 283
SchI	GAGTC	5 cut(s)	792, 1408, 1465, 2686, 2750
ScrFI	CCNGG	3 cut(s)	1311, 2410, 2436
SduI	GDGCHC	3 cut(s)	1284, 1695, 1952
SfcI	CTRYAG	4 cut(s)	223, 318, 527, 1344
Sfr274I	CTCGAG	1 cut(s)	843
SfuI	TTCGAA	1 cut(s)	1667
SinI	GGWCC	2 cut(s)	238, 283
SlaI	CTCGAG	1 cut(s)	843
SmiMI	CAYNNNNRTG	1 cut(s)	814
SmlI	CTYRAG	2 cut(s)	843, 1004
SmoI	CTYRAG	2 cut(s)	843, 1004
SsiI	CCGC	7 cut(s)	1203, 1508, 1875, 1942, 2077, 2595, 2703
SspI	AATATT	2 cut(s)	2353, 2659
SspMI	CTAG	5 cut(s)	770, 1526, 1697, 2201, 2844
SstI	GAGCTC	1 cut(s)	1695
StyD4I	CCNGG	3 cut(s)	1309, 2408, 2434
StyI	CCWWGG	1 cut(s)	827
TaaI	ACNGT	8 cut(s)	52, 92, 358, 1027, 1552, 1889, 1979, 2142
TaiI	ACGT	3 cut(s)	14, 294, 2631
TaqI	TCGA	6 cut(s)	130, 844, 996, 1667, 2144, 2323
TauI	GCSGC	2 cut(s)	1945, 2597
TfiI	GAWTC	5 cut(s)	97, 179, 566, 1790, 2446
Tru1I	TTAA	9 cut(s)	218, 609, 678, 1104, 1274, 1704, 2178, 2268, 2914
Tru9I	TTAA	9 cut(s)	218, 609, 678, 1104, 1274, 1704, 2178, 2268, 2914
TscAI	CASTG	4 cut(s)	914, 1555, 1894, 2871
TseFI	GTSAC	4 cut(s)	571, 837, 1411, 2071
TseI	GCWGC	4 cut(s)	317, 1334, 2801, 2860
Tsp45I	GTSAC	4 cut(s)	571, 837, 1411, 2071
TspGWI	ACGGA	6 cut(s)	230, 467, 590, 1019, 2468, 2834
TspRI	CASTG	4 cut(s)	914, 1555, 1894, 2871
VpaK11BI	GGWCC	2 cut(s)	238, 283
XapI	RAATTY	7 cut(s)	545, 1109, 1151, 1172, 1422, 1670, 2270
XceI	RCATGY	1 cut(s)	1488
XcmI	CCANNNNNNNNNTGG	1 cut(s)	29
XhoI	CTCGAG	1 cut(s)	843
XmiI	GTMKAC	1 cut(s)	130
XmnI	GAANNNNTTC	1 cut(s)	1379
XspI	CTAG	5 cut(s)	770, 1526, 1697, 2201, 2844
Zsp2I	ATGCAT	1 cut(s)	375

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.